RLG00000036458

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005677
Physical Location & Seq
Reverse (-)
80880188 .. 80884315
4128 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036458

Sequence Viewer

Length: 1332 bp
ATGGAGAGCTACTTGCACCTCTCGCTGGAGGCCTCAAGCAATTGGAAGAAATTCGTCGACCTCGTCGTCAAGAACCCCACCAGAAAGTTCCGACATGTCGTCGATCTTGCTAAGCAGGCTGAGGCTGAGAAGAAGAAGACAACGACGACATTGATTTTGCACCTTGCCGGAATTCCGATCAACGGCTTGGCGGCACAGCTTTTGCTTCTCAGCCATTGCGGAACTCTTCGATCCCGTCTGTTGGAAGCACTTTGCTCACCGCCGAGGTCTTTGACGGACGGACAACGCCGGCTTCAGAATCAGAAGCATAAGCTTGAGAAGATTACTTTACTGGAAGGACATGCTAACATTGGGAAAACACCTGTTTATGATGACTCATCAACAAACGAAGAATCTGCAATGAAATCTAAGGACATGGACATGACAATGAACAATGATGAATTGAGGGAAATGATCGATGAGATCAATAAGAGAATCCAATCGAAATCGGACAGAATCCCAGTTGTTAAAACCCAACAGTCGAGCTTTCCAACTACTCGGGAATCGAGAAGAGAGTCTGAGTGTGAGTGCTTCAATCTGTACAAGGAGCTAGTTCCTTACCGGCAAGCTTGGTCTTGGCAGAAGAGCATTGTCAAGGAGAAAAAGGCTCTGATTGAGAGGAATCAGGACTGCCCCGACTCGCTGTTTGTTCTTCAGCATCCTCCTGTTTATACAATGGGCACCGGTAGCTCAGAAAAGTTCCTCAATTTTGACCTCAAGGATGCTCCATTTGATGTTCATCGGACCGAACGCGGCGGCGAGGTTACATATCATGGTCCTGGTCAGATAGTAATGTACCCCATTATGAATCTTCGAAATCACAAAATGGATCTTCATTGGTACCTCAGGTCACTCGAGGAGGTGATCATTCGCGCTCTTTCAAAGACATTTTCGATCAAGGCATCTCGACTTGAGGGCTTAACTGGTGTTTGGCATGGAAACCAGAAACTGGCAGCCATTGGTATACGAGTGTCTCAGTGGATAGCTTATCACGGCTTGGCACTGAACGTAACCACAGATTTAACTCCCTTTCGTTGGATAGTACCGTGTGGTTTACAAAACTATCAGGTTGGAAGCATTAGAGGGTTGTTGGGGGAGTTTGAGTCATTTGACAATAGTAGTAGAAAACAGCTACCAGATCCTGATGATGGCCAGCTACTTGATATTGCTTGTAAATCTTTGATCAATGAGTTTTCAGAAGTTTTTCAGGTTGGAATCAATTATGAAACCATCTCGAGGTTAGAGTTTTTAGAAAGTGAACCGGTAAAGCCTCTAAAAGAGGAAGTAATTTAG

Protein Analysis

444

Amino Acids

50.63

Weight (kDa)

7.65

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 200 - 379 9.8e-40 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
Acc65I GGTACC 1 cut(s) 879
AccB1I GGYRCC 2 cut(s) 719, 879
AccB7I CCANNNNNTGG 1 cut(s) 988
AccI GTMKAC 2 cut(s) 57, 1003
AccII CGCG 2 cut(s) 792, 912
AciI CCGC 5 cut(s) 191, 219, 260, 792, 795
AclWI GGATC 3 cut(s) 225, 876, 1172
AcoI YGGCCR 1 cut(s) 1189
AcsI RAATTY 2 cut(s) 50, 171
AcuI CTGAAG 2 cut(s) 278, 677
AfaI GTAC 4 cut(s) 581, 836, 881, 1083
AfiI CCNNNNNNNGG 7 cut(s) 25, 182, 241, 988, 1074, 1187, 1275
AflIII ACRYGT 1 cut(s) 94
AgeI ACCGGT 2 cut(s) 722, 1300
AgsI TTSAA 2 cut(s) 574, 921
AhdI GACNNNNNGTC 1 cut(s) 98
AjnI CCWGG 1 cut(s) 817
Alw26I GTCTC 1 cut(s) 1017
AlwI GGATC 3 cut(s) 225, 876, 1172
AlwNI CAGNNNCTG 2 cut(s) 988, 1181
Ama87I CYCGRG 3 cut(s) 537, 893, 1273
AoxI GGCC 2 cut(s) 30, 1189
ApeKI GCWGC 1 cut(s) 992
ApoI RAATTY 2 cut(s) 50, 171
ArsI GACNNNNNNTTYG 4 cut(s) 139, 171, 668, 700
AsiGI ACCGGT 2 cut(s) 722, 1300
Asp700I GAANNNNTTC 2 cut(s) 50, 1242
Asp718I GGTACC 1 cut(s) 879
AspLEI GCGC 1 cut(s) 914
AspS9I GGNCC 2 cut(s) 783, 815
AsuHPI GGTGA 2 cut(s) 249, 913
AsuII TTCGAA 1 cut(s) 853
AvaI CYCGRG 3 cut(s) 537, 893, 1273
AvaII GGWCC 2 cut(s) 783, 815
AxyI CCTNAGG 1 cut(s) 884
BaeGI GKGCMC 1 cut(s) 722
BalI TGGCCA 1 cut(s) 1191
BanI GGYRCC 2 cut(s) 719, 879
BbsI GAAGAC 1 cut(s) 143
BbvCI CCTCAGC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 1004
BccI CCATC 2 cut(s) 1181, 1277
BceAI ACGGC 2 cut(s) 199, 1048
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BciT130I CCWGG 1 cut(s) 819
BclI TGATCA 2 cut(s) 903, 1221
BcoDI GTCTC 1 cut(s) 1017
BfaI CTAG 1 cut(s) 590
BisI GCNGC 4 cut(s) 192, 793, 796, 993
BlpI GCTNAGC 1 cut(s) 111
BlsI GCNGC 4 cut(s) 193, 794, 797, 994
Bme1390I CCNGG 1 cut(s) 819
Bme18I GGWCC 2 cut(s) 783, 815
BmeRI GACNNNNNGTC 1 cut(s) 98
BmeT110I CYCGRG 3 cut(s) 537, 893, 1273
BmgT120I GGNCC 2 cut(s) 783, 815
BmiI GGNNCC 2 cut(s) 721, 881
BmrFI CCNGG 1 cut(s) 819
BmrI ACTGGG 1 cut(s) 494
BmsI GCATC 3 cut(s) 706, 751, 950
BmuI ACTGGG 1 cut(s) 494
BpiI GAAGAC 1 cut(s) 143
BpmI CTGGAG 1 cut(s) 47
Bpu10I CCTNAGC 1 cut(s) 120
Bpu1102I GCTNAGC 1 cut(s) 111
Bpu14I TTCGAA 1 cut(s) 853
BpuEI CTTGAG 4 cut(s) 19, 335, 740, 971
Bsa29I ATCGAT 1 cut(s) 456
BsaBI GATNNNNATC 1 cut(s) 777
BsaJI CCNNGG 1 cut(s) 263
BsaWI WCCGGW 2 cut(s) 722, 1300
BsaXI ACNNNNNCTCC 2 cut(s) 1127, 1157
Bsc4I CCNNNNNNNGG 7 cut(s) 25, 182, 241, 988, 1074, 1187, 1275
Bse118I RCCGGY 4 cut(s) 288, 600, 722, 1300
Bse1I ACTGG 4 cut(s) 336, 500, 967, 993
Bse21I CCTNAGG 1 cut(s) 884
Bse3DI GCAATG 2 cut(s) 214, 405
Bse8I GATNNNNATC 1 cut(s) 777
BseBI CCWGG 1 cut(s) 819
BseCI ATCGAT 1 cut(s) 456
BseDI CCNNGG 1 cut(s) 263
BseGI GGATG 2 cut(s) 697, 766
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 7 cut(s) 25, 182, 241, 988, 1074, 1187, 1275
BseMI GCAATG 2 cut(s) 214, 405
BseMII CTCAG 7 cut(s) 111, 117, 223, 549, 744, 898, 1028
BseNI ACTGG 4 cut(s) 336, 500, 967, 993
BseRI GAGGAG 1 cut(s) 911
BseSI GKGCMC 1 cut(s) 722
BseXI GCAGC 1 cut(s) 1004
Bsh1236I CGCG 2 cut(s) 792, 912
BshFI GGCC 2 cut(s) 32, 1191
BshNI GGYRCC 2 cut(s) 719, 879
BshTI ACCGGT 2 cut(s) 722, 1300
BshVI ATCGAT 1 cut(s) 456
BsiHKCI CYCGRG 3 cut(s) 537, 893, 1273
BsiSI CCGG 5 cut(s) 168, 289, 601, 723, 1301
BslI CCNNNNNNNGG 7 cut(s) 25, 182, 241, 988, 1074, 1187, 1275
BsmAI GTCTC 1 cut(s) 1017
BsnI GGCC 2 cut(s) 32, 1191
BsoBI CYCGRG 3 cut(s) 537, 893, 1273
Bsp119I TTCGAA 1 cut(s) 853
Bsp1286I GDGCHC 1 cut(s) 722
Bsp1407I TGTACA 1 cut(s) 579
Bsp1720I GCTNAGC 1 cut(s) 111
BspACI CCGC 5 cut(s) 191, 219, 260, 792, 795
BspANI GGCC 2 cut(s) 32, 1191
BspCNI CTCAG 7 cut(s) 112, 118, 222, 550, 743, 897, 1027
BspDI ATCGAT 1 cut(s) 456
BspFNI CGCG 2 cut(s) 792, 912
BspLI GGNNCC 2 cut(s) 721, 881
BspPI GGATC 3 cut(s) 225, 876, 1172
BspQI GCTCTTC 1 cut(s) 617
BspT104I TTCGAA 1 cut(s) 853
BspT107I GGYRCC 2 cut(s) 719, 879
BsrDI GCAATG 2 cut(s) 214, 405
BsrFI RCCGGY 4 cut(s) 288, 600, 722, 1300
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 4 cut(s) 336, 500, 967, 993
BssAI RCCGGY 4 cut(s) 288, 600, 722, 1300
BssECI CCNNGG 1 cut(s) 263
BssNAI GTATAC 1 cut(s) 1004
Bst1107I GTATAC 1 cut(s) 1004
Bst2UI CCWGG 1 cut(s) 819
Bst4CI ACNGT 2 cut(s) 519, 1086
Bst6I CTCTTC 3 cut(s) 231, 544, 617
BstAUI TGTACA 1 cut(s) 579
BstBI TTCGAA 1 cut(s) 853
BstC8I GCNNGC 4 cut(s) 117, 290, 606, 1193
BstDEI CTNAG 9 cut(s) 111, 120, 126, 209, 408, 558, 730, 884, 1014
BstF5I GGATG 2 cut(s) 697, 766
BstFNI CGCG 2 cut(s) 792, 912
BstHHI GCGC 1 cut(s) 914
BstMAI GTCTC 1 cut(s) 1017
BstMWI GCNNNNNNNGC 3 cut(s) 22, 116, 726
BstNI CCWGG 1 cut(s) 819
BstNSI RCATGY 2 cut(s) 98, 344
BstSCI CCNGG 1 cut(s) 817
BstSLI GKGCMC 1 cut(s) 722
BstUI CGCG 2 cut(s) 792, 912
BstV1I GCAGC 1 cut(s) 1004
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 2 cut(s) 868, 1177
BstYI RGATCY 2 cut(s) 868, 1177
BstZ17I GTATAC 1 cut(s) 1004
Bsu15I ATCGAT 1 cut(s) 456
Bsu36I CCTNAGG 1 cut(s) 884
BsuRI GGCC 2 cut(s) 32, 1191
BsuTUI ATCGAT 1 cut(s) 456
BtsCI GGATG 2 cut(s) 697, 766
BtsIMutI CAGTG 2 cut(s) 1022, 1040
Cac8I GCNNGC 4 cut(s) 117, 290, 606, 1193
CaiI CAGNNNCTG 2 cut(s) 988, 1181
CfoI GCGC 1 cut(s) 914
Cfr10I RCCGGY 4 cut(s) 288, 600, 722, 1300
Cfr13I GGNCC 2 cut(s) 783, 815
ClaI ATCGAT 1 cut(s) 456
CpoI CGGWCCG 1 cut(s) 783
Csp6I GTAC 4 cut(s) 580, 835, 880, 1082
CspAI ACCGGT 2 cut(s) 722, 1300
CspI CGGWCCG 1 cut(s) 783
CviAII CATG 6 cut(s) 95, 341, 415, 421, 812, 974
CviQI GTAC 4 cut(s) 580, 835, 880, 1082
DdeI CTNAG 9 cut(s) 111, 120, 126, 209, 408, 558, 730, 884, 1014
DrdI GACNNNNNNGTC 1 cut(s) 65
DriI GACNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 1 cut(s) 1189
Eam1104I CTCTTC 3 cut(s) 231, 544, 617
Eam1105I GACNNNNNGTC 1 cut(s) 98
EarI CTCTTC 3 cut(s) 231, 544, 617
Eco147I AGGCCT 1 cut(s) 32
Eco47I GGWCC 2 cut(s) 783, 815
Eco57I CTGAAG 2 cut(s) 278, 677
Eco81I CCTNAGG 1 cut(s) 884
Eco88I CYCGRG 3 cut(s) 537, 893, 1273
EcoRI GAATTC 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 817
FaeI CATG 6 cut(s) 98, 344, 418, 424, 815, 977
FatI CATG 6 cut(s) 94, 340, 414, 420, 811, 973
FbaI TGATCA 2 cut(s) 903, 1221
FblI GTMKAC 2 cut(s) 57, 1003
Fnu4HI GCNGC 4 cut(s) 192, 793, 796, 993
FokI GGATG 2 cut(s) 684, 773
Fsp4HI GCNGC 4 cut(s) 192, 793, 796, 993
FspBI CTAG 1 cut(s) 590
GlaI GCGC 1 cut(s) 913
GluI GCNGC 4 cut(s) 192, 793, 796, 993
GsuI CTGGAG 1 cut(s) 47
HaeIII GGCC 2 cut(s) 32, 1191
HapII CCGG 5 cut(s) 168, 289, 601, 723, 1301
HhaI GCGC 1 cut(s) 914
Hin1II CATG 6 cut(s) 98, 344, 418, 424, 815, 977
Hin6I GCGC 1 cut(s) 912
HinP1I GCGC 1 cut(s) 912
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 2 cut(s) 311, 606
HpaII CCGG 5 cut(s) 168, 289, 601, 723, 1301
HphI GGTGA 2 cut(s) 249, 913
Hpy166II GTNNAC 4 cut(s) 58, 1004, 1094, 1298
Hpy188III TCNNGA 7 cut(s) 70, 539, 546, 665, 945, 1181, 1273
Hpy8I GTNNAC 4 cut(s) 58, 1004, 1094, 1298
Hpy99I CGWCG 4 cut(s) 59, 68, 104, 148
HpyAV CCTTC 1 cut(s) 329
HpyCH4III ACNGT 2 cut(s) 519, 1086
HpyCH4IV ACGT 1 cut(s) 1047
HpyCH4V TGCA 3 cut(s) 16, 160, 398
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 116, 726
HpyF3I CTNAG 9 cut(s) 111, 120, 126, 209, 408, 558, 730, 884, 1014
HpySE526I ACGT 1 cut(s) 1047
Hsp92II CATG 6 cut(s) 98, 344, 418, 424, 815, 977
HspAI GCGC 1 cut(s) 912
KpnI GGTACC 1 cut(s) 883
KroI GCCGGC 1 cut(s) 288
KroNI GCCGGC 1 cut(s) 290
Ksp22I TGATCA 2 cut(s) 903, 1221
LguI GCTCTTC 1 cut(s) 617
LmnI GCTCC 2 cut(s) 586, 769
Lsp1109I GCAGC 1 cut(s) 1004
LweI GCATC 3 cut(s) 706, 751, 950
MaeI CTAG 1 cut(s) 590
MaeII ACGT 1 cut(s) 1047
MaeIII GTNAC 3 cut(s) 802, 888, 1048
MfeI CAATTG 1 cut(s) 40
MflI RGATCY 2 cut(s) 868, 1177
MhlI GDGCHC 1 cut(s) 722
MlsI TGGCCA 1 cut(s) 1191
MluCI AATT 7 cut(s) 40, 50, 171, 440, 745, 1258, 1326
MluNI TGGCCA 1 cut(s) 1191
MlyI GAGTC 4 cut(s) 368, 563, 671, 1151
MmeI TCCRAC 6 cut(s) 115, 222, 554, 1055, 1090, 1231
Mox20I TGGCCA 1 cut(s) 1191
MroNI GCCGGC 1 cut(s) 288
MroXI GAANNNNTTC 2 cut(s) 50, 1242
MscI TGGCCA 1 cut(s) 1191
MseI TTAA 3 cut(s) 507, 959, 1061
MslI CAYNNNNRTG 2 cut(s) 419, 425
Msp20I TGGCCA 1 cut(s) 1191
MspI CCGG 5 cut(s) 168, 289, 601, 723, 1301
MspR9I CCNGG 1 cut(s) 819
MunI CAATTG 1 cut(s) 40
MvaI CCWGG 1 cut(s) 819
MvnI CGCG 2 cut(s) 792, 912
MwoI GCNNNNNNNGC 3 cut(s) 22, 116, 726
NaeI GCCGGC 1 cut(s) 290
NgoMIV GCCGGC 1 cut(s) 288
NlaIII CATG 6 cut(s) 98, 344, 418, 424, 815, 977
NlaIV GGNNCC 2 cut(s) 721, 881
NmeAIII GCCGAG 1 cut(s) 288
NmuCI GTSAC 1 cut(s) 888
NspI RCATGY 2 cut(s) 98, 344
NspV TTCGAA 1 cut(s) 853
PaeR7I CTCGAG 2 cut(s) 893, 1273
PceI AGGCCT 1 cut(s) 32
PciI ACATGT 1 cut(s) 94
PciSI GCTCTTC 1 cut(s) 617
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 63
PdiI GCCGGC 1 cut(s) 290
PdmI GAANNNNTTC 2 cut(s) 50, 1242
PfeI GAWTC 8 cut(s) 298, 392, 474, 495, 542, 661, 847, 1254
PflFI GACNNNGTC 1 cut(s) 62
PflMI CCANNNNNTGG 1 cut(s) 988
PinAI ACCGGT 2 cut(s) 722, 1300
PkrI GCNGC 4 cut(s) 193, 794, 797, 994
PleI GAGTC 4 cut(s) 368, 562, 671, 1150
PpsI GAGTC 4 cut(s) 368, 562, 671, 1150
PscI ACATGT 1 cut(s) 94
Psp6I CCWGG 1 cut(s) 817
PspGI CCWGG 1 cut(s) 817
PspN4I GGNNCC 2 cut(s) 721, 881
PspPI GGNCC 2 cut(s) 783, 815
PspXI VCTCGAGB 1 cut(s) 893
PstNI CAGNNNCTG 2 cut(s) 988, 1181
PsuI RGATCY 2 cut(s) 868, 1177
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 4 cut(s) 581, 836, 881, 1083
RsaNI GTAC 4 cut(s) 580, 835, 880, 1082
RseI CAYNNNNRTG 2 cut(s) 419, 425
Rsr2I CGGWCCG 1 cut(s) 783
RsrII CGGWCCG 1 cut(s) 783
SalI GTCGAC 1 cut(s) 56
SapI GCTCTTC 1 cut(s) 617
SaqAI TTAA 3 cut(s) 507, 959, 1061
SatI GCNGC 4 cut(s) 192, 793, 796, 993
Sau96I GGNCC 2 cut(s) 783, 815
SchI GAGTC 4 cut(s) 368, 563, 671, 1151
ScrFI CCNGG 1 cut(s) 819
SduI GDGCHC 1 cut(s) 722
SfaNI GCATC 3 cut(s) 706, 751, 950
Sfr274I CTCGAG 2 cut(s) 893, 1273
SfuI TTCGAA 1 cut(s) 853
SinI GGWCC 2 cut(s) 783, 815
SlaI CTCGAG 2 cut(s) 893, 1273
SmiMI CAYNNNNRTG 2 cut(s) 419, 425
SmlI CTYRAG 6 cut(s) 34, 314, 755, 893, 950, 1273
SmoI CTYRAG 6 cut(s) 34, 314, 755, 893, 950, 1273
Sse9I AATT 7 cut(s) 40, 50, 171, 440, 745, 1258, 1326
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 5 cut(s) 191, 219, 260, 792, 795
SspMI CTAG 1 cut(s) 590
StuI AGGCCT 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 817
TaaI ACNGT 2 cut(s) 519, 1086
TaiI ACGT 1 cut(s) 1050
TaqII GACCGA 1 cut(s) 800
TasI AATT 7 cut(s) 40, 50, 171, 440, 745, 1258, 1326
TatI WGTACW 1 cut(s) 579
TauI GCSGC 3 cut(s) 194, 795, 798
TfiI GAWTC 8 cut(s) 298, 392, 474, 495, 542, 661, 847, 1254
Tru1I TTAA 3 cut(s) 507, 959, 1061
Tru9I TTAA 3 cut(s) 507, 959, 1061
TscAI CASTG 2 cut(s) 1022, 1047
TseFI GTSAC 1 cut(s) 888
TseI GCWGC 1 cut(s) 992
Tsp45I GTSAC 1 cut(s) 888
TspDTI ATGAA 7 cut(s) 416, 443, 453, 767, 860, 863, 1278
TspGWI ACGGA 2 cut(s) 290, 294
TspRI CASTG 2 cut(s) 1022, 1047
Tth111I GACNNNGTC 1 cut(s) 62
Van91I CCANNNNNTGG 1 cut(s) 988
VpaK11BI GGWCC 2 cut(s) 783, 815
XapI RAATTY 2 cut(s) 50, 171
XceI RCATGY 2 cut(s) 98, 344
XhoI CTCGAG 2 cut(s) 893, 1273
XmiI GTMKAC 2 cut(s) 57, 1003
XmnI GAANNNNTTC 2 cut(s) 50, 1242
XspI CTAG 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.