Rroxscaffold_1G00007250

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005677
Physical Location & Seq
Reverse (-)
9277407 .. 9278634
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00007250.1

Sequence Viewer

Length: 948 bp
ATGACTTTCTTGGCGACCCAATGTGTCGTCGCCTCAATCCCAACATACCCAGTTTCTAATCATCAGCCCAAACGGTGCCTCTCCCAGAGAATCCAATCGAAATCGGACAGAATCCCAGTTGTTAAAACCCAACAGTCGAGCTTTCCAACTACTCAGGAATCGAGAAGAGAGTCTGAGTGTGAGTGCTTCAATCTGTACAAGGAGCTAGTTCCTTACCGGCAAGCTTGGTCTTGGCAGAAGAGCATTGTCAAGGAGAAAAAGGCTCTGATTGAGAGGAATCAGGACTGCCCGGACTCGCTGTTTGTTCTTCAGCATCCTCCTGTTTATACAATGGGCACCGGTAGCTCAGAAAAGTTCCTCAATTTTGACCTCAAGGATGCTCCATTTGATGTTCATCGGACCGAACGCGGCGGCGAGGTTACATATCACGGTCCTGGTCAGATAGTAATGTACCCCATTATGAATCTCCGAAATCACAAAATGGATCTTCATTGGTACCTCAGGTCACTTGAGGAGGTGATCATTCGCGCTCTTTCAAAGACATTTTCGATCAAGGCATCTCGACTTGAGGGCTTAACTGGTGTTTGGCATGGAAACCAGAAACTGGCGGCCATTGGTATACGAGTGTCTCAGTGGATAGCTTATCACGGTTTGGCACTGAACGTAACCACAGATTTAACTCCTTTTCGTTGGATAGTACCGTGTGGTTTACAAAACTATCAGGTTGGAAGCATTAGAGGGTTGTTGGGGGAGTTTGAGTCATTTGACAATAGTAGTAGAAAACAGCTACCAGATCCTGATGATGGCCAGCTACTTGATATTGCTTGTAAATCTTTGATCAATGAGTTTTCAGAAGTTTTTCAGGTTGGAATCAATTATGAAACCATCTCGAGGTCAGAGTTTTTAGAAAGTGAACCGGTAAAGCCTCTAAAAGAGGAAGTAATTTAG

Protein Analysis

315

Amino Acids

36.04

Weight (kDa)

7.08

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 72 - 251 4.2e-40 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 495
AccB1I GGYRCC 3 cut(s) 75, 335, 495
AccB7I CCANNNNNTGG 1 cut(s) 604
AccI GTMKAC 1 cut(s) 619
AccII CGCG 2 cut(s) 408, 528
AciI CCGC 3 cut(s) 408, 411, 608
AclWI GGATC 2 cut(s) 492, 788
AcoI YGGCCR 2 cut(s) 609, 805
AcuI CTGAAG 1 cut(s) 293
AfaI GTAC 4 cut(s) 197, 452, 497, 699
AfiI CCNNNNNNNGG 3 cut(s) 604, 803, 891
AgeI ACCGGT 2 cut(s) 338, 916
AgsI TTSAA 2 cut(s) 190, 537
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 7 cut(s) 141, 205, 224, 345, 641, 787, 811
AluI AGCT 7 cut(s) 141, 205, 224, 345, 641, 787, 811
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 2 cut(s) 492, 788
AlwNI CAGNNNCTG 2 cut(s) 604, 797
Ama87I CYCGRG 1 cut(s) 889
AoxI GGCC 2 cut(s) 609, 805
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AsiGI ACCGGT 2 cut(s) 338, 916
Asp700I GAANNNNTTC 1 cut(s) 858
Asp718I GGTACC 1 cut(s) 495
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 2 cut(s) 399, 431
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 529
AvaI CYCGRG 1 cut(s) 889
AvaII GGWCC 2 cut(s) 399, 431
AxyI CCTNAGG 1 cut(s) 500
BaeGI GKGCMC 1 cut(s) 338
BalI TGGCCA 1 cut(s) 807
BanI GGYRCC 3 cut(s) 75, 335, 495
BccI CCATC 2 cut(s) 797, 893
BciT130I CCWGG 1 cut(s) 435
BclI TGATCA 2 cut(s) 519, 837
BcnI CCSGG 1 cut(s) 290
BcoDI GTCTC 1 cut(s) 633
BfaI CTAG 1 cut(s) 206
BisI GCNGC 3 cut(s) 409, 412, 609
BlsI GCNGC 3 cut(s) 410, 413, 610
Bme1390I CCNGG 2 cut(s) 290, 435
Bme18I GGWCC 2 cut(s) 399, 431
BmeT110I CYCGRG 1 cut(s) 889
BmgT120I GGNCC 2 cut(s) 399, 431
BmiI GGNNCC 3 cut(s) 77, 337, 497
BmrFI CCNGG 2 cut(s) 290, 435
BmrI ACTGGG 2 cut(s) 44, 110
BmsI GCATC 3 cut(s) 322, 367, 566
BmuI ACTGGG 2 cut(s) 44, 110
BpuEI CTTGAG 3 cut(s) 356, 530, 587
BpuMI CCSGG 1 cut(s) 290
BsaBI GATNNNNATC 1 cut(s) 393
BsaWI WCCGGW 2 cut(s) 338, 916
BsaXI ACNNNNNCTCC 2 cut(s) 743, 773
Bsc4I CCNNNNNNNGG 3 cut(s) 604, 803, 891
Bse118I RCCGGY 3 cut(s) 216, 338, 916
Bse1I ACTGG 4 cut(s) 50, 116, 583, 609
Bse21I CCTNAGG 1 cut(s) 500
Bse8I GATNNNNATC 1 cut(s) 393
BseBI CCWGG 1 cut(s) 435
BseGI GGATG 2 cut(s) 313, 382
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 3 cut(s) 604, 803, 891
BseMII CTCAG 5 cut(s) 165, 167, 360, 514, 644
BseNI ACTGG 4 cut(s) 50, 116, 583, 609
BseRI GAGGAG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 338
Bsh1236I CGCG 2 cut(s) 408, 528
BshFI GGCC 2 cut(s) 611, 807
BshNI GGYRCC 3 cut(s) 75, 335, 495
BshTI ACCGGT 2 cut(s) 338, 916
BsiHKCI CYCGRG 1 cut(s) 889
BsiSI CCGG 4 cut(s) 217, 290, 339, 917
BslI CCNNNNNNNGG 3 cut(s) 604, 803, 891
BsmAI GTCTC 1 cut(s) 633
BsnI GGCC 2 cut(s) 611, 807
BsoBI CYCGRG 1 cut(s) 889
Bsp1286I GDGCHC 1 cut(s) 338
Bsp1407I TGTACA 1 cut(s) 195
Bsp143I GATC 5 cut(s) 484, 519, 549, 793, 837
BspACI CCGC 3 cut(s) 408, 411, 608
BspANI GGCC 2 cut(s) 611, 807
BspCNI CTCAG 5 cut(s) 166, 166, 359, 513, 643
BspFNI CGCG 2 cut(s) 408, 528
BspLI GGNNCC 3 cut(s) 77, 337, 497
BspPI GGATC 2 cut(s) 492, 788
BspQI GCTCTTC 1 cut(s) 233
BspT107I GGYRCC 3 cut(s) 75, 335, 495
BsrFI RCCGGY 3 cut(s) 216, 338, 916
BsrGI TGTACA 1 cut(s) 195
BsrI ACTGG 4 cut(s) 50, 116, 583, 609
BssAI RCCGGY 3 cut(s) 216, 338, 916
BssMI GATC 5 cut(s) 484, 519, 549, 793, 837
BssNAI GTATAC 1 cut(s) 620
Bst1107I GTATAC 1 cut(s) 620
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 5 cut(s) 75, 135, 431, 650, 702
Bst6I CTCTTC 2 cut(s) 160, 233
BstAUI TGTACA 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 222, 809
BstDEI CTNAG 5 cut(s) 153, 174, 346, 500, 630
BstF5I GGATG 2 cut(s) 313, 382
BstFNI CGCG 2 cut(s) 408, 528
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 5 cut(s) 487, 522, 552, 796, 840
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 5 cut(s) 484, 519, 549, 793, 837
BstMWI GCNNNNNNNGC 1 cut(s) 342
BstNI CCWGG 1 cut(s) 435
BstSCI CCNGG 2 cut(s) 288, 433
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 2 cut(s) 408, 528
BstX2I RGATCY 2 cut(s) 484, 793
BstYI RGATCY 2 cut(s) 484, 793
BstZ17I GTATAC 1 cut(s) 620
Bsu36I CCTNAGG 1 cut(s) 500
BsuRI GGCC 2 cut(s) 611, 807
BtsCI GGATG 2 cut(s) 313, 382
BtsIMutI CAGTG 2 cut(s) 638, 656
Cac8I GCNNGC 2 cut(s) 222, 809
CaiI CAGNNNCTG 2 cut(s) 604, 797
CfoI GCGC 1 cut(s) 530
Cfr10I RCCGGY 3 cut(s) 216, 338, 916
Cfr13I GGNCC 2 cut(s) 399, 431
CpoI CGGWCCG 1 cut(s) 399
Csp6I GTAC 4 cut(s) 196, 451, 496, 698
CspAI ACCGGT 2 cut(s) 338, 916
CspI CGGWCCG 1 cut(s) 399
CviAII CATG 1 cut(s) 590
CviQI GTAC 4 cut(s) 196, 451, 496, 698
DdeI CTNAG 5 cut(s) 153, 174, 346, 500, 630
DpnI GATC 5 cut(s) 486, 521, 551, 795, 839
DpnII GATC 5 cut(s) 484, 519, 549, 793, 837
EaeI YGGCCR 2 cut(s) 609, 805
Eam1104I CTCTTC 2 cut(s) 160, 233
EarI CTCTTC 2 cut(s) 160, 233
Eco47I GGWCC 2 cut(s) 399, 431
Eco57I CTGAAG 1 cut(s) 293
Eco81I CCTNAGG 1 cut(s) 500
Eco88I CYCGRG 1 cut(s) 889
EcoRII CCWGG 1 cut(s) 433
FaeI CATG 1 cut(s) 593
FaiI YATR 7 cut(s) 46, 327, 424, 461, 591, 620, 879
FatI CATG 1 cut(s) 589
FbaI TGATCA 2 cut(s) 519, 837
FblI GTMKAC 1 cut(s) 619
Fnu4HI GCNGC 3 cut(s) 409, 412, 609
FokI GGATG 2 cut(s) 300, 389
Fsp4HI GCNGC 3 cut(s) 409, 412, 609
FspBI CTAG 1 cut(s) 206
GlaI GCGC 1 cut(s) 529
GluI GCNGC 3 cut(s) 409, 412, 609
HaeIII GGCC 2 cut(s) 611, 807
HapII CCGG 4 cut(s) 217, 290, 339, 917
HhaI GCGC 1 cut(s) 530
Hin1II CATG 1 cut(s) 593
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HindIII AAGCTT 1 cut(s) 222
HinfI GANTC 9 cut(s) 90, 111, 158, 170, 277, 293, 463, 758, 870
HpaII CCGG 4 cut(s) 217, 290, 339, 917
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 3 cut(s) 620, 710, 914
Hpy188I TCNGA 9 cut(s) 106, 175, 267, 349, 399, 441, 470, 853, 898
Hpy188III TCNNGA 6 cut(s) 155, 162, 281, 561, 797, 889
Hpy8I GTNNAC 3 cut(s) 620, 710, 914
Hpy99I CGWCG 1 cut(s) 32
HpyCH4III ACNGT 5 cut(s) 75, 135, 431, 650, 702
HpyCH4IV ACGT 1 cut(s) 663
HpyF10VI GCNNNNNNNGC 1 cut(s) 342
HpyF3I CTNAG 5 cut(s) 153, 174, 346, 500, 630
HpySE526I ACGT 1 cut(s) 663
Hsp92II CATG 1 cut(s) 593
HspAI GCGC 1 cut(s) 528
KpnI GGTACC 1 cut(s) 499
Ksp22I TGATCA 2 cut(s) 519, 837
Kzo9I GATC 5 cut(s) 484, 519, 549, 793, 837
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 2 cut(s) 202, 385
LweI GCATC 3 cut(s) 322, 367, 566
MaeI CTAG 1 cut(s) 206
MaeII ACGT 1 cut(s) 663
MaeIII GTNAC 3 cut(s) 418, 504, 664
MalI GATC 5 cut(s) 486, 521, 551, 795, 839
MboI GATC 5 cut(s) 484, 519, 549, 793, 837
MboII GAAGA 4 cut(s) 177, 250, 299, 479
MflI RGATCY 2 cut(s) 484, 793
MhlI GDGCHC 1 cut(s) 338
MlsI TGGCCA 1 cut(s) 807
MluCI AATT 3 cut(s) 361, 874, 942
MluNI TGGCCA 1 cut(s) 807
MlyI GAGTC 3 cut(s) 179, 287, 767
MmeI TCCRAC 4 cut(s) 170, 671, 706, 847
Mox20I TGGCCA 1 cut(s) 807
MroXI GAANNNNTTC 1 cut(s) 858
MscI TGGCCA 1 cut(s) 807
MseI TTAA 3 cut(s) 123, 575, 677
Msp20I TGGCCA 1 cut(s) 807
MspI CCGG 4 cut(s) 217, 290, 339, 917
MspR9I CCNGG 2 cut(s) 290, 435
MvaI CCWGG 1 cut(s) 435
MvnI CGCG 2 cut(s) 408, 528
MwoI GCNNNNNNNGC 1 cut(s) 342
NciI CCSGG 1 cut(s) 290
NdeII GATC 5 cut(s) 484, 519, 549, 793, 837
NlaIII CATG 1 cut(s) 593
NlaIV GGNNCC 3 cut(s) 77, 337, 497
NmuCI GTSAC 1 cut(s) 504
PaeR7I CTCGAG 1 cut(s) 889
PciSI GCTCTTC 1 cut(s) 233
PdmI GAANNNNTTC 1 cut(s) 858
PfeI GAWTC 6 cut(s) 90, 111, 158, 277, 463, 870
PflMI CCANNNNNTGG 1 cut(s) 604
PinAI ACCGGT 2 cut(s) 338, 916
PkrI GCNGC 3 cut(s) 410, 413, 610
PleI GAGTC 3 cut(s) 178, 287, 766
PpsI GAGTC 3 cut(s) 178, 287, 766
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 3 cut(s) 77, 337, 497
PspPI GGNCC 2 cut(s) 399, 431
PstNI CAGNNNCTG 2 cut(s) 604, 797
PsuI RGATCY 2 cut(s) 484, 793
RsaI GTAC 4 cut(s) 197, 452, 497, 699
RsaNI GTAC 4 cut(s) 196, 451, 496, 698
Rsr2I CGGWCCG 1 cut(s) 399
RsrII CGGWCCG 1 cut(s) 399
SapI GCTCTTC 1 cut(s) 233
SaqAI TTAA 3 cut(s) 123, 575, 677
SatI GCNGC 3 cut(s) 409, 412, 609
Sau3AI GATC 5 cut(s) 484, 519, 549, 793, 837
Sau96I GGNCC 2 cut(s) 399, 431
SchI GAGTC 3 cut(s) 179, 287, 767
ScrFI CCNGG 2 cut(s) 290, 435
SduI GDGCHC 1 cut(s) 338
SfaNI GCATC 3 cut(s) 322, 367, 566
Sfr274I CTCGAG 1 cut(s) 889
SinI GGWCC 2 cut(s) 399, 431
SlaI CTCGAG 1 cut(s) 889
SmlI CTYRAG 4 cut(s) 371, 509, 566, 889
SmoI CTYRAG 4 cut(s) 371, 509, 566, 889
Sse9I AATT 3 cut(s) 361, 874, 942
SsiI CCGC 3 cut(s) 408, 411, 608
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 2 cut(s) 288, 433
TaaI ACNGT 5 cut(s) 75, 135, 431, 650, 702
TaiI ACGT 1 cut(s) 666
TaqI TCGA 6 cut(s) 98, 137, 161, 548, 562, 890
TaqII GACCGA 1 cut(s) 416
TasI AATT 3 cut(s) 361, 874, 942
TatI WGTACW 1 cut(s) 195
TauI GCSGC 3 cut(s) 411, 414, 611
TfiI GAWTC 6 cut(s) 90, 111, 158, 277, 463, 870
Tru1I TTAA 3 cut(s) 123, 575, 677
Tru9I TTAA 3 cut(s) 123, 575, 677
TscAI CASTG 2 cut(s) 638, 663
TseFI GTSAC 1 cut(s) 504
Tsp45I GTSAC 1 cut(s) 504
TspDTI ATGAA 4 cut(s) 383, 476, 479, 894
TspRI CASTG 2 cut(s) 638, 663
Van91I CCANNNNNTGG 1 cut(s) 604
VpaK11BI GGWCC 2 cut(s) 399, 431
XhoI CTCGAG 1 cut(s) 889
XmiI GTMKAC 1 cut(s) 619
XmnI GAANNNNTTC 1 cut(s) 858
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.