MD06G1010800.v1.1

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005677
Physical Location & Seq
Forward (+)
1258804 .. 1261663
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1010800.v1.1.491

Sequence Viewer

Length: 909 bp
ATGACTCTTGTGGCGACTCACTGTGTTGCTGCAGTTCCAACATACCCAATCTATAAGCATCAAAATAAACCTCCTCGGTCTTTCTCTCTGCGCATCTTATCAAAATTGAATCAAGTTTCAGATGTTAAAACTCCGCAGTCCACCTCTTATACTGTCGAGCCAATGAGAGAGTGTGAGTGCTTCAATATGTATAAGCAACAGGTTCCTTATAGTCAGGCATGGGCTTGGCAGAAGAGCATAGTTGAGGAGAAAAAAGCTATGATAGAAAAGAATGATCATTGCCCAGACTCATTGTTCGTTCTTCAGCATTGTCCTGTGTATACCTTGGGAACTGCTAGTTCAGAACAGTTCCTTAATTTTGATATCAAGGACCCACCTTTCGACGTTTATCGAACTGAACGTGGCGGCGAGGTTACATATCATGGTCCGGGTCAGATAGTTATGTACCCTATTATGAATCTACGAAATCACAAGATGGATCTTCATTGGTACCTCAGGTCACTTGAGGAGGTGATCATCCGTGCTCTATCAAAGACATTCTGTATCAAGGCATCTCGGCTTGAGGGCCTCACTGGTGTTTGGCATGGAAACCAGAAACTGGCAGCCATTGGAATACGAGTCAACCAGTGGATAACATATCATGGCTTAGCAGTGAATGTGAACCCAGACTTGACCCCCTTTCGTTGGATCGTACCTTGTGGGTTACAAGGCTATCAGGTTGGAAGCGTTAAAAGCTTGCTTGAGGAATTTCATACATCTGCAGACTGTGGAAGAGCAGATCTACCTGATCCCAATGATGGTCAGCTACTTGATATTACTTGTAGATCTTTGATCAATGAGTTTTCTGAAGTTTTTGAGGTTAGAATCAATTATGAAACCATGTCAAGGTTGGATTTACAAGCCGTCTGA

Protein Analysis

303

Amino Acids

34.56

Weight (kDa)

6.55

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 70 - 250 1.7e-38 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 92
Acc65I GGTACC 1 cut(s) 489
AccB1I GGYRCC 1 cut(s) 489
AccB7I CCANNNNNTGG 1 cut(s) 598
AccI GTMKAC 1 cut(s) 320
AciI CCGC 2 cut(s) 134, 405
AclWI GGATC 3 cut(s) 486, 695, 782
AcsI RAATTY 1 cut(s) 746
AcuI CTGAAG 2 cut(s) 287, 867
AfaI GTAC 3 cut(s) 446, 491, 693
AfiI CCNNNNNNNGG 4 cut(s) 598, 684, 797, 885
AgsI TTSAA 2 cut(s) 109, 184
AluBI AGCT 3 cut(s) 257, 735, 805
AluI AGCT 3 cut(s) 257, 735, 805
Alw21I GWGCWC 1 cut(s) 526
AlwI GGATC 3 cut(s) 486, 695, 782
AlwNI CAGNNNCTG 1 cut(s) 598
AoxI GGCC 1 cut(s) 565
ApeKI GCWGC 2 cut(s) 29, 602
ApoI RAATTY 1 cut(s) 746
ArsI GACNNNNNNTTYG 4 cut(s) 278, 310, 362, 394
Asp718I GGTACC 1 cut(s) 489
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 3 cut(s) 370, 425, 565
AsuC2I CCSGG 1 cut(s) 429
AsuHPI GGTGA 1 cut(s) 523
AvaII GGWCC 2 cut(s) 370, 425
AxyI CCTNAGG 1 cut(s) 494
BanI GGYRCC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 526
BbvI GCAGC 2 cut(s) 16, 614
BccI CCATC 2 cut(s) 469, 791
BceAI ACGGC 1 cut(s) 887
BclI TGATCA 3 cut(s) 274, 513, 831
BcnI CCSGG 1 cut(s) 429
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 2 cut(s) 30, 759
BglII AGATCT 2 cut(s) 778, 824
BisI GCNGC 3 cut(s) 30, 406, 603
BlpI GCTNAGC 1 cut(s) 646
BlsI GCNGC 3 cut(s) 31, 407, 604
Bme1390I CCNGG 1 cut(s) 429
Bme18I GGWCC 2 cut(s) 370, 425
BmgT120I GGNCC 3 cut(s) 370, 425, 565
BmiI GGNNCC 3 cut(s) 204, 372, 491
BmrFI CCNGG 1 cut(s) 429
BmsI GCATC 3 cut(s) 67, 102, 560
Bpu1102I GCTNAGC 1 cut(s) 646
BpuEI CTTGAG 3 cut(s) 524, 581, 761
BpuMI CCSGG 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 74, 324
Bsc4I CCNNNNNNNGG 4 cut(s) 598, 684, 797, 885
Bse1I ACTGG 3 cut(s) 577, 603, 625
Bse21I CCTNAGG 1 cut(s) 494
Bse3DI GCAATG 1 cut(s) 277
BseDI CCNNGG 2 cut(s) 74, 324
BseGI GGATG 1 cut(s) 516
BseLI CCNNNNNNNGG 4 cut(s) 598, 684, 797, 885
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 508
BseNI ACTGG 3 cut(s) 577, 603, 625
BseRI GAGGAG 3 cut(s) 63, 260, 521
BseXI GCAGC 2 cut(s) 16, 614
BshFI GGCC 1 cut(s) 567
BshNI GGYRCC 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 526
BsiSI CCGG 1 cut(s) 428
BslI CCNNNNNNNGG 4 cut(s) 598, 684, 797, 885
BsnI GGCC 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 526
Bsp143I GATC 8 cut(s) 274, 478, 513, 687, 778, 787, 824, 831
Bsp1720I GCTNAGC 1 cut(s) 646
BspACI CCGC 2 cut(s) 134, 405
BspANI GGCC 1 cut(s) 567
BspCNI CTCAG 1 cut(s) 507
BspLI GGNNCC 3 cut(s) 204, 372, 491
BspMAI CTGCAG 2 cut(s) 34, 763
BspPI GGATC 3 cut(s) 486, 695, 782
BspQI GCTCTTC 2 cut(s) 227, 766
BspT107I GGYRCC 1 cut(s) 489
BsrDI GCAATG 1 cut(s) 277
BsrI ACTGG 3 cut(s) 577, 603, 625
BssECI CCNNGG 2 cut(s) 74, 324
BssMI GATC 8 cut(s) 274, 478, 513, 687, 778, 787, 824, 831
BssNAI GTATAC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 324
Bst1107I GTATAC 1 cut(s) 321
Bst4CI ACNGT 4 cut(s) 23, 154, 348, 767
Bst6I CTCTTC 2 cut(s) 227, 766
BstC8I GCNNGC 1 cut(s) 737
BstDEI CTNAG 2 cut(s) 494, 646
BstF5I GGATG 1 cut(s) 516
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 8 cut(s) 277, 481, 516, 690, 781, 790, 827, 834
BstMBI GATC 8 cut(s) 274, 478, 513, 687, 778, 787, 824, 831
BstMWI GCNNNNNNNGC 1 cut(s) 732
BstSCI CCNGG 1 cut(s) 427
BstSFI CTRYAG 2 cut(s) 30, 759
BstV1I GCAGC 2 cut(s) 16, 614
BstX2I RGATCY 3 cut(s) 478, 778, 824
BstYI RGATCY 3 cut(s) 478, 778, 824
BstZ17I GTATAC 1 cut(s) 321
Bsu36I CCTNAGG 1 cut(s) 494
BsuRI GGCC 1 cut(s) 567
BtsCI GGATG 1 cut(s) 516
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 4 cut(s) 19, 570, 632, 657
Cac8I GCNNGC 1 cut(s) 737
CaiI CAGNNNCTG 1 cut(s) 598
CfoI GCGC 1 cut(s) 93
Cfr13I GGNCC 3 cut(s) 370, 425, 565
Csp6I GTAC 3 cut(s) 445, 490, 692
CviAII CATG 5 cut(s) 219, 422, 584, 641, 880
CviQI GTAC 3 cut(s) 445, 490, 692
DdeI CTNAG 2 cut(s) 494, 646
DpnI GATC 8 cut(s) 276, 480, 515, 689, 780, 789, 826, 833
DpnII GATC 8 cut(s) 274, 478, 513, 687, 778, 787, 824, 831
Eam1104I CTCTTC 2 cut(s) 227, 766
EarI CTCTTC 2 cut(s) 227, 766
Eco130I CCWWGG 1 cut(s) 324
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 370, 425
Eco57I CTGAAG 2 cut(s) 287, 867
Eco81I CCTNAGG 1 cut(s) 494
EcoO109I RGGNCCY 2 cut(s) 370, 565
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 5 cut(s) 222, 425, 587, 644, 883
FatI CATG 5 cut(s) 218, 421, 583, 640, 879
FbaI TGATCA 3 cut(s) 274, 513, 831
FblI GTMKAC 1 cut(s) 320
Fnu4HI GCNGC 3 cut(s) 30, 406, 603
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 3 cut(s) 30, 406, 603
FspBI CTAG 1 cut(s) 336
FspI TGCGCA 1 cut(s) 92
GlaI GCGC 1 cut(s) 92
GluI GCNGC 3 cut(s) 30, 406, 603
HaeIII GGCC 1 cut(s) 567
HapII CCGG 1 cut(s) 428
HhaI GCGC 1 cut(s) 93
Hin1II CATG 5 cut(s) 222, 425, 587, 644, 883
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HindIII AAGCTT 1 cut(s) 733
HinfI GANTC 7 cut(s) 4, 16, 109, 287, 457, 618, 864
HpaII CCGG 1 cut(s) 428
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 4 cut(s) 141, 321, 622, 661
Hpy188I TCNGA 5 cut(s) 121, 343, 435, 847, 908
Hpy8I GTNNAC 4 cut(s) 141, 321, 622, 661
Hpy99I CGWCG 1 cut(s) 386
HpyCH4III ACNGT 4 cut(s) 23, 154, 348, 767
HpyCH4IV ACGT 2 cut(s) 384, 400
HpyCH4V TGCA 2 cut(s) 32, 761
HpyF10VI GCNNNNNNNGC 1 cut(s) 732
HpyF3I CTNAG 2 cut(s) 494, 646
HpySE526I ACGT 2 cut(s) 384, 400
Hsp92II CATG 5 cut(s) 222, 425, 587, 644, 883
HspAI GCGC 1 cut(s) 91
KpnI GGTACC 1 cut(s) 493
Ksp22I TGATCA 3 cut(s) 274, 513, 831
Kzo9I GATC 8 cut(s) 274, 478, 513, 687, 778, 787, 824, 831
LguI GCTCTTC 2 cut(s) 227, 766
Lsp1109I GCAGC 2 cut(s) 16, 614
LweI GCATC 3 cut(s) 67, 102, 560
MaeI CTAG 1 cut(s) 336
MaeII ACGT 2 cut(s) 384, 400
MaeIII GTNAC 3 cut(s) 412, 498, 702
MalI GATC 8 cut(s) 276, 480, 515, 689, 780, 789, 826, 833
MboI GATC 8 cut(s) 274, 478, 513, 687, 778, 787, 824, 831
MboII GAAGA 4 cut(s) 244, 293, 473, 783
MflI RGATCY 3 cut(s) 478, 778, 824
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 4 cut(s) 104, 355, 746, 868
MlyI GAGTC 3 cut(s) 10, 281, 627
MmeI TCCRAC 4 cut(s) 62, 665, 700, 870
MseI TTAA 3 cut(s) 126, 354, 729
MspI CCGG 1 cut(s) 428
MspR9I CCNGG 1 cut(s) 429
MwoI GCNNNNNNNGC 1 cut(s) 732
NciI CCSGG 1 cut(s) 429
NdeII GATC 8 cut(s) 274, 478, 513, 687, 778, 787, 824, 831
NlaIII CATG 5 cut(s) 222, 425, 587, 644, 883
NlaIV GGNNCC 3 cut(s) 204, 372, 491
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 498
NsbI TGCGCA 1 cut(s) 92
PciSI GCTCTTC 2 cut(s) 227, 766
PcsI WCGNNNNNNNCGW 1 cut(s) 397
PfeI GAWTC 3 cut(s) 109, 457, 864
PflMI CCANNNNNTGG 1 cut(s) 598
PkrI GCNGC 3 cut(s) 31, 407, 604
PleI GAGTC 3 cut(s) 10, 281, 626
PpsI GAGTC 3 cut(s) 10, 281, 626
PpuMI RGGWCCY 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 370
PspN4I GGNNCC 3 cut(s) 204, 372, 491
PspPI GGNCC 3 cut(s) 370, 425, 565
PspPPI RGGWCCY 1 cut(s) 370
PstI CTGCAG 2 cut(s) 34, 763
PstNI CAGNNNCTG 1 cut(s) 598
PsuI RGATCY 3 cut(s) 478, 778, 824
RsaI GTAC 3 cut(s) 446, 491, 693
RsaNI GTAC 3 cut(s) 445, 490, 692
SapI GCTCTTC 2 cut(s) 227, 766
SaqAI TTAA 3 cut(s) 126, 354, 729
SatI GCNGC 3 cut(s) 30, 406, 603
Sau3AI GATC 8 cut(s) 274, 478, 513, 687, 778, 787, 824, 831
Sau96I GGNCC 3 cut(s) 370, 425, 565
SchI GAGTC 3 cut(s) 10, 281, 627
ScrFI CCNGG 1 cut(s) 429
SduI GDGCHC 1 cut(s) 526
SfaNI GCATC 3 cut(s) 67, 102, 560
SfcI CTRYAG 2 cut(s) 30, 759
SinI GGWCC 2 cut(s) 370, 425
SmlI CTYRAG 3 cut(s) 503, 560, 740
SmoI CTYRAG 3 cut(s) 503, 560, 740
Sse9I AATT 4 cut(s) 104, 355, 746, 868
SsiI CCGC 2 cut(s) 134, 405
SspMI CTAG 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 427
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 4 cut(s) 23, 154, 348, 767
TaiI ACGT 2 cut(s) 387, 403
TaqI TCGA 3 cut(s) 156, 381, 391
TaqII GACCGA 1 cut(s) 66
TasI AATT 4 cut(s) 104, 355, 746, 868
TauI GCSGC 1 cut(s) 408
TfiI GAWTC 3 cut(s) 109, 457, 864
Tru1I TTAA 3 cut(s) 126, 354, 729
Tru9I TTAA 3 cut(s) 126, 354, 729
TscAI CASTG 4 cut(s) 26, 577, 632, 657
TseFI GTSAC 1 cut(s) 498
TseI GCWGC 2 cut(s) 29, 602
Tsp45I GTSAC 1 cut(s) 498
TspDTI ATGAA 4 cut(s) 470, 473, 740, 888
TspGWI ACGGA 1 cut(s) 509
TspRI CASTG 4 cut(s) 26, 577, 632, 657
Van91I CCANNNNNTGG 1 cut(s) 598
VpaK11BI GGWCC 2 cut(s) 370, 425
XapI RAATTY 1 cut(s) 746
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.