FvH4_2g26090

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005677
Physical Location & Seq
Reverse (-)
20991732 .. 20993544
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26090.t1

Sequence Viewer

Length: 681 bp
ATGACTTTCATGGCTACCCAATCTTTCGCCGCCTCAATCCCACCGTACCCAGTTTCCAATCACCACCCTAAACCTCCTCGGCTCCTCTCCCCCAGAATCCAACCAATCCCAGCCGTTAAAACCCAGCACTGTGAGTGCTTCAATCTGTACAAGGAGCTAGTTCCTTACAGGCAAGCCTGGTCTTGGCAGAAGAGCTTAGTCAAAGAGAAGAAGGCTCTGATTGAGAGGAATAAGCACTGCCCAGACTCGCTCATTGTTCTTCAGCATCCGCCTGTTTATACAATGGGCACCGGCAGCTCCGAGAAGTTCCTCAATTTCGACATCACGGATGCGCCGTTTGATGTTCACCGCACCGAGCGCGGCGGCGAGGTTACATATCATGGTCCTGGTCAGATAGTAATGTACCCCATTATGAATCTCCGGAATCACAAAATGGATCTTCATTGGTACCTCAGGTCACTTGAGGAGGTGATCATTCGCGCTCTTTCAAACACATTTTCTATCAAGGCCTCTCGGCTTGAGGGCTTAACTGGTGTTTGGCATGAAACTAGCAGCCATTGGTATACGAGTGTCTCAGTGGATAGCTTATCACGGTCTAGCAGTGAACGTGACCACAGATTTAACTCCTTTTGGTTGGATAGTACCGTGTGGTTTGCAAAACTATCAGGTCGGAAGCATTAG

Protein Analysis

227

Amino Acids

26.16

Weight (kDa)

9.36

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 56 - 182 1.4e-23 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 447
AccB1I GGYRCC 2 cut(s) 287, 447
AccI GTMKAC 1 cut(s) 563
AccII CGCG 2 cut(s) 360, 480
AccIII TCCGGA 1 cut(s) 420
AciI CCGC 5 cut(s) 30, 269, 349, 360, 363
AclWI GGATC 1 cut(s) 444
AcuI CTGAAG 1 cut(s) 245
AfaI GTAC 5 cut(s) 47, 149, 404, 449, 643
AfiI CCNNNNNNNGG 1 cut(s) 183
AgsI TTSAA 2 cut(s) 142, 489
AjnI CCWGG 2 cut(s) 176, 385
AluBI AGCT 4 cut(s) 157, 195, 297, 585
AluI AGCT 4 cut(s) 157, 195, 297, 585
Alw26I GTCTC 1 cut(s) 577
AlwI GGATC 1 cut(s) 444
Aor13HI TCCGGA 1 cut(s) 420
AoxI GGCC 1 cut(s) 507
ApeKI GCWGC 2 cut(s) 294, 552
Asp718I GGTACC 1 cut(s) 447
AspLEI GCGC 3 cut(s) 334, 360, 482
AspS9I GGNCC 1 cut(s) 383
AsuHPI GGTGA 3 cut(s) 53, 338, 481
AvaII GGWCC 1 cut(s) 383
AxyI CCTNAGG 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 290
BanI GGYRCC 2 cut(s) 287, 447
BbvI GCAGC 2 cut(s) 306, 564
BceAI ACGGC 2 cut(s) 98, 319
BciT130I CCWGG 2 cut(s) 178, 387
BclI TGATCA 1 cut(s) 471
BcoDI GTCTC 1 cut(s) 577
BfaI CTAG 3 cut(s) 158, 549, 597
BisI GCNGC 5 cut(s) 30, 295, 361, 364, 553
BlsI GCNGC 5 cut(s) 31, 296, 362, 365, 554
Bme1390I CCNGG 2 cut(s) 178, 387
Bme18I GGWCC 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 3 cut(s) 83, 289, 449
BmrFI CCNGG 2 cut(s) 178, 387
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 2 cut(s) 274, 319
BmuI ACTGGG 1 cut(s) 44
BpuEI CTTGAG 2 cut(s) 482, 539
BsaJI CCNNGG 1 cut(s) 77
BsaWI WCCGGW 1 cut(s) 420
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 2 cut(s) 50, 535
Bse21I CCTNAGG 1 cut(s) 452
BseAI TCCGGA 1 cut(s) 420
BseBI CCWGG 2 cut(s) 178, 387
BseDI CCNNGG 1 cut(s) 77
BseGI GGATG 2 cut(s) 265, 334
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMII CTCAG 2 cut(s) 466, 588
BseNI ACTGG 2 cut(s) 50, 535
BseRI GAGGAG 3 cut(s) 66, 74, 479
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 2 cut(s) 306, 564
BseYI CCCAGC 2 cut(s) 109, 123
Bsh1236I CGCG 2 cut(s) 360, 480
BshFI GGCC 1 cut(s) 509
BshNI GGYRCC 2 cut(s) 287, 447
BsiSI CCGG 2 cut(s) 291, 421
BslI CCNNNNNNNGG 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 577
BsnI GGCC 1 cut(s) 509
Bsp1286I GDGCHC 1 cut(s) 290
Bsp13I TCCGGA 1 cut(s) 420
Bsp1407I TGTACA 1 cut(s) 147
Bsp143I GATC 2 cut(s) 436, 471
BspACI CCGC 5 cut(s) 30, 269, 349, 360, 363
BspANI GGCC 1 cut(s) 509
BspCNI CTCAG 2 cut(s) 465, 587
BspEI TCCGGA 1 cut(s) 420
BspFNI CGCG 2 cut(s) 360, 480
BspLI GGNNCC 3 cut(s) 83, 289, 449
BspPI GGATC 1 cut(s) 444
BspQI GCTCTTC 1 cut(s) 185
BspT107I GGYRCC 2 cut(s) 287, 447
BsrFI RCCGGY 1 cut(s) 290
BsrGI TGTACA 1 cut(s) 147
BsrI ACTGG 2 cut(s) 50, 535
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 77
BssMI GATC 2 cut(s) 436, 471
BssNAI GTATAC 1 cut(s) 564
Bst1107I GTATAC 1 cut(s) 564
Bst2UI CCWGG 2 cut(s) 178, 387
Bst4CI ACNGT 4 cut(s) 45, 131, 594, 646
Bst6I CTCTTC 1 cut(s) 185
BstAUI TGTACA 1 cut(s) 147
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 3 cut(s) 196, 452, 574
BstF5I GGATG 2 cut(s) 265, 334
BstFNI CGCG 2 cut(s) 360, 480
BstHHI GCGC 3 cut(s) 334, 360, 482
BstKTI GATC 2 cut(s) 439, 474
BstMAI GTCTC 1 cut(s) 577
BstMBI GATC 2 cut(s) 436, 471
BstMWI GCNNNNNNNGC 2 cut(s) 294, 357
BstNI CCWGG 2 cut(s) 178, 387
BstSCI CCNGG 2 cut(s) 176, 385
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 2 cut(s) 360, 480
BstV1I GCAGC 2 cut(s) 306, 564
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BstZ17I GTATAC 1 cut(s) 564
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 1 cut(s) 509
BtsCI GGATG 2 cut(s) 265, 334
BtsI GCAGTG 2 cut(s) 235, 607
BtsIMutI CAGTG 4 cut(s) 127, 235, 582, 607
Cac8I GCNNGC 1 cut(s) 174
CfoI GCGC 3 cut(s) 334, 360, 482
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 383
Csp6I GTAC 5 cut(s) 46, 148, 403, 448, 642
CviAII CATG 3 cut(s) 10, 380, 542
CviQI GTAC 5 cut(s) 46, 148, 403, 448, 642
DdeI CTNAG 3 cut(s) 196, 452, 574
DpnI GATC 2 cut(s) 438, 473
DpnII GATC 2 cut(s) 436, 471
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
EciI GGCGGA 1 cut(s) 258
Eco147I AGGCCT 1 cut(s) 509
Eco47I GGWCC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 245
Eco81I CCTNAGG 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 176, 385
FaeI CATG 3 cut(s) 13, 383, 545
FaiI YATR 7 cut(s) 11, 279, 376, 381, 413, 543, 564
FatI CATG 3 cut(s) 9, 379, 541
FbaI TGATCA 1 cut(s) 471
FblI GTMKAC 1 cut(s) 563
Fnu4HI GCNGC 5 cut(s) 30, 295, 361, 364, 553
FokI GGATG 2 cut(s) 252, 341
Fsp4HI GCNGC 5 cut(s) 30, 295, 361, 364, 553
FspBI CTAG 3 cut(s) 158, 549, 597
GlaI GCGC 3 cut(s) 333, 359, 481
GluI GCNGC 5 cut(s) 30, 295, 361, 364, 553
GsaI CCCAGC 2 cut(s) 113, 127
HaeIII GGCC 1 cut(s) 509
HapII CCGG 2 cut(s) 291, 421
HhaI GCGC 3 cut(s) 334, 360, 482
Hin1II CATG 3 cut(s) 13, 383, 545
Hin6I GCGC 3 cut(s) 332, 358, 480
HinP1I GCGC 3 cut(s) 332, 358, 480
HinfI GANTC 4 cut(s) 96, 245, 415, 424
HpaII CCGG 2 cut(s) 291, 421
HphI GGTGA 3 cut(s) 53, 338, 481
Hpy166II GTNNAC 3 cut(s) 346, 564, 605
Hpy188I TCNGA 4 cut(s) 219, 301, 393, 672
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 3 cut(s) 346, 564, 605
HpyAV CCTTC 1 cut(s) 205
HpyCH4III ACNGT 4 cut(s) 45, 131, 594, 646
HpyCH4IV ACGT 1 cut(s) 607
HpyCH4V TGCA 1 cut(s) 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 294, 357
HpyF3I CTNAG 3 cut(s) 196, 452, 574
HpySE526I ACGT 1 cut(s) 607
Hsp92II CATG 3 cut(s) 13, 383, 545
HspAI GCGC 3 cut(s) 332, 358, 480
Kpn2I TCCGGA 1 cut(s) 420
KpnI GGTACC 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 471
Kzo9I GATC 2 cut(s) 436, 471
LguI GCTCTTC 1 cut(s) 185
LmnI GCTCC 3 cut(s) 87, 154, 302
Lsp1109I GCAGC 2 cut(s) 306, 564
LweI GCATC 2 cut(s) 274, 319
MaeI CTAG 3 cut(s) 158, 549, 597
MaeII ACGT 1 cut(s) 607
MaeIII GTNAC 3 cut(s) 370, 456, 608
MalI GATC 2 cut(s) 438, 473
MboI GATC 2 cut(s) 436, 471
MboII GAAGA 4 cut(s) 202, 220, 251, 431
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 1 cut(s) 290
MluCI AATT 1 cut(s) 313
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 3 cut(s) 124, 615, 650
MroI TCCGGA 1 cut(s) 420
MseI TTAA 3 cut(s) 117, 527, 621
MspI CCGG 2 cut(s) 291, 421
MspR9I CCNGG 2 cut(s) 178, 387
MvaI CCWGG 2 cut(s) 178, 387
MvnI CGCG 2 cut(s) 360, 480
MwoI GCNNNNNNNGC 2 cut(s) 294, 357
NdeII GATC 2 cut(s) 436, 471
NlaIII CATG 3 cut(s) 13, 383, 545
NlaIV GGNNCC 3 cut(s) 83, 289, 449
NmeAIII GCCGAG 2 cut(s) 58, 493
NmuCI GTSAC 2 cut(s) 456, 608
PceI AGGCCT 1 cut(s) 509
PciSI GCTCTTC 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 332
PfeI GAWTC 3 cut(s) 96, 415, 424
PkrI GCNGC 5 cut(s) 31, 296, 362, 365, 554
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 176, 385
PspFI CCCAGC 2 cut(s) 109, 123
PspGI CCWGG 2 cut(s) 176, 385
PspN4I GGNNCC 3 cut(s) 83, 289, 449
PspPI GGNCC 1 cut(s) 383
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 5 cut(s) 47, 149, 404, 449, 643
RsaNI GTAC 5 cut(s) 46, 148, 403, 448, 642
SapI GCTCTTC 1 cut(s) 185
SaqAI TTAA 3 cut(s) 117, 527, 621
SatI GCNGC 5 cut(s) 30, 295, 361, 364, 553
Sau3AI GATC 2 cut(s) 436, 471
Sau96I GGNCC 1 cut(s) 383
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 2 cut(s) 178, 387
SduI GDGCHC 1 cut(s) 290
SfaNI GCATC 2 cut(s) 274, 319
SinI GGWCC 1 cut(s) 383
SmlI CTYRAG 2 cut(s) 461, 518
SmoI CTYRAG 2 cut(s) 461, 518
Sse9I AATT 1 cut(s) 313
SseBI AGGCCT 1 cut(s) 509
SsiI CCGC 5 cut(s) 30, 269, 349, 360, 363
SspMI CTAG 3 cut(s) 158, 549, 597
StuI AGGCCT 1 cut(s) 509
StyD4I CCNGG 2 cut(s) 176, 385
TaaI ACNGT 4 cut(s) 45, 131, 594, 646
TaiI ACGT 1 cut(s) 610
TaqI TCGA 1 cut(s) 318
TasI AATT 1 cut(s) 313
TatI WGTACW 1 cut(s) 147
TauI GCSGC 3 cut(s) 32, 363, 366
TfiI GAWTC 3 cut(s) 96, 415, 424
Tru1I TTAA 3 cut(s) 117, 527, 621
Tru9I TTAA 3 cut(s) 117, 527, 621
TscAI CASTG 4 cut(s) 134, 242, 582, 607
TseFI GTSAC 2 cut(s) 456, 608
TseI GCWGC 2 cut(s) 294, 552
Tsp45I GTSAC 2 cut(s) 456, 608
TspDTI ATGAA 3 cut(s) 428, 431, 558
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 4 cut(s) 134, 242, 582, 607
VpaK11BI GGWCC 1 cut(s) 383
XmiI GTMKAC 1 cut(s) 563
XspI CTAG 3 cut(s) 158, 549, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.