Rh5DG520100

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005677
Physical Location & Seq
Forward (+)
80743636 .. 80817319
73684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG520100.1

Sequence Viewer

Length: 1200 bp
ATGGGTTTGGGTTTGTCTCGAATGAAACAAAGGCGTGAAATTGTTGACGAGGGGAACAGCAAGACCTCTTGTGCTGCGGCTGAGCATCATCTTCACATTCAACAATCGCTTCAAGTTTCTGCTCTCAGCTTTGACTTTGATTGGATTTCCGATATTATCGTCACCTATGACCTACCAATTAATTTTCGCTACGAACTCTCGATATTTCCGTTACTTTTCGCTATACTTCAGGCTTTTCGATTTCGTGCCATGTCACCGACTTCAAATTGGTGGCTCTGGAGTTGTGATTGTCTGGTGTTCTTCTTCGAAGTTCGAACTACAGTGACTGCAAATATGCCTAGAATCCAATCGAAATCGGACAGAATCCCAGTTGTTAAAACCCAACAGTCGAGCTTTCCAACTACTCAGGAATCGAGAAGAGAGTCTGAGTGTGAGTGCTTCAATCTGTACAAGGAGCTAGTTCCTTACCGGCAAGCTTGGTCTTGGCAGAAGAGCATTGTCAAGGAGAAAAAGGCTCTGATCGAGAGGAATCAGGACTGCCCGGACTCGCTGTTTGTTCTTCAGCATCCTCCTGTTTATACAATGGGCACCGGTAGCTCAGAAAAGTTCCTCAATTTTGACCTCAAGGATGCTCCATTTGATGTTCACCGGACCGAACGCGGCGGCGAGGTTACATATCACGGTCCTGGTCAGATAGTAATGTACCCCATTATGAATCTCCGAAATCACAAAATGGATCTTCATTGGTACCTCAGGTCACTTGAGGAGGTGATCATTCGCGCTCTTTCAAAGACATTTTCGATCAAGGCATCTCGACTTGAGGGCTTAACTGGTGTTTGGCATGGAAACCAAAAACTGGCAGCCATTGGTATACGAGTATCTCAGTGGATAGCTTATCACGGTTTGGCACTGAACGTAACCACATATTTAACCCCTTTTCGTTGGATAGTACCGTGTGGTTTACAAAACTATCAGGTTGGAAGCATTAGAGGGTTGTTGGGGGAGTTTGAGTCATTTGACAATAGTAGTAGAAAACAACTACCAGATCCTGATGATGGCCAGCTACTTGATATTGCTTGTAAATCTTTGATCAATGAGTTTTCAGAAGTTTTTCAGGTTGGAATCAATTATAAAGCCATCTCGACGTTAGAGTTTTTAGAAAGTGAACCGGTAAAGCCTCTAAAAGAGGAATTAATTTAG

Protein Analysis

399

Amino Acids

45.96

Weight (kDa)

6.57

Isoelectric Point (pI)

51.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 156 - 335 1.6e-38 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1131
Acc65I GGTACC 1 cut(s) 747
AccB1I GGYRCC 2 cut(s) 587, 747
AccB7I CCANNNNNTGG 1 cut(s) 856
AccI GTMKAC 1 cut(s) 871
AccII CGCG 2 cut(s) 660, 780
AciI CCGC 3 cut(s) 77, 660, 663
AclWI GGATC 2 cut(s) 744, 1040
AcoI YGGCCR 1 cut(s) 1057
AcuI CTGAAG 2 cut(s) 212, 545
AfaI GTAC 4 cut(s) 449, 704, 749, 951
AfiI CCNNNNNNNGG 2 cut(s) 856, 1055
AgeI ACCGGT 2 cut(s) 590, 1168
AgsI TTSAA 5 cut(s) 101, 113, 264, 442, 789
AjnI CCWGG 1 cut(s) 685
AluBI AGCT 7 cut(s) 129, 393, 457, 476, 597, 893, 1063
AluI AGCT 7 cut(s) 129, 393, 457, 476, 597, 893, 1063
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 2 cut(s) 744, 1040
AlwNI CAGNNNCTG 2 cut(s) 326, 1049
AoxI GGCC 1 cut(s) 1057
ApeKI GCWGC 2 cut(s) 74, 860
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AseI ATTAAT 2 cut(s) 180, 1193
AsiGI ACCGGT 2 cut(s) 590, 1168
Asp700I GAANNNNTTC 1 cut(s) 1110
Asp718I GGTACC 1 cut(s) 747
AspLEI GCGC 1 cut(s) 782
AspS9I GGNCC 2 cut(s) 651, 683
AsuC2I CCSGG 1 cut(s) 542
AsuHPI GGTGA 4 cut(s) 154, 246, 638, 781
AsuII TTCGAA 2 cut(s) 306, 313
AvaII GGWCC 2 cut(s) 651, 683
AxyI CCTNAGG 1 cut(s) 752
BaeGI GKGCMC 1 cut(s) 590
BalI TGGCCA 1 cut(s) 1059
BanI GGYRCC 2 cut(s) 587, 747
BbvI GCAGC 2 cut(s) 61, 872
BccI CCATC 2 cut(s) 1049, 1145
BciT130I CCWGG 1 cut(s) 687
BclI TGATCA 2 cut(s) 771, 1089
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 2 cut(s) 339, 458
BfmI CTRYAG 1 cut(s) 318
BisI GCNGC 5 cut(s) 75, 78, 661, 664, 861
BlpI GCTNAGC 1 cut(s) 81
BlsI GCNGC 5 cut(s) 76, 79, 662, 665, 862
Bme1390I CCNGG 2 cut(s) 542, 687
Bme18I GGWCC 2 cut(s) 651, 683
BmgT120I GGNCC 2 cut(s) 651, 683
BmiI GGNNCC 2 cut(s) 589, 749
BmrFI CCNGG 2 cut(s) 542, 687
BmrI ACTGGG 1 cut(s) 362
BmsI GCATC 4 cut(s) 94, 574, 619, 818
BmuI ACTGGG 1 cut(s) 362
BpmI CTGGAG 1 cut(s) 298
Bpu1102I GCTNAGC 1 cut(s) 81
Bpu14I TTCGAA 2 cut(s) 306, 313
BpuEI CTTGAG 3 cut(s) 608, 782, 839
BpuMI CCSGG 1 cut(s) 542
BsaWI WCCGGW 3 cut(s) 590, 648, 1168
BsaXI ACNNNNNCTCC 2 cut(s) 995, 1025
Bsc4I CCNNNNNNNGG 2 cut(s) 856, 1055
Bse118I RCCGGY 3 cut(s) 468, 590, 1168
Bse1I ACTGG 3 cut(s) 368, 835, 861
Bse21I CCTNAGG 1 cut(s) 752
BseBI CCWGG 1 cut(s) 687
BseGI GGATG 2 cut(s) 565, 634
BseLI CCNNNNNNNGG 2 cut(s) 856, 1055
BseMII CTCAG 7 cut(s) 72, 139, 417, 419, 612, 766, 896
BseNI ACTGG 3 cut(s) 368, 835, 861
BseRI GAGGAG 1 cut(s) 779
BseSI GKGCMC 1 cut(s) 590
BseXI GCAGC 2 cut(s) 61, 872
Bsh1236I CGCG 2 cut(s) 660, 780
BshFI GGCC 1 cut(s) 1059
BshNI GGYRCC 2 cut(s) 587, 747
BshTI ACCGGT 2 cut(s) 590, 1168
BsiSI CCGG 5 cut(s) 469, 542, 591, 649, 1169
BslI CCNNNNNNNGG 2 cut(s) 856, 1055
BsmAI GTCTC 1 cut(s) 21
BsnI GGCC 1 cut(s) 1059
Bsp119I TTCGAA 2 cut(s) 306, 313
Bsp1286I GDGCHC 1 cut(s) 590
Bsp1407I TGTACA 1 cut(s) 447
Bsp143I GATC 6 cut(s) 519, 736, 771, 801, 1045, 1089
Bsp1720I GCTNAGC 1 cut(s) 81
BspACI CCGC 3 cut(s) 77, 660, 663
BspANI GGCC 1 cut(s) 1059
BspCNI CTCAG 7 cut(s) 73, 138, 418, 418, 611, 765, 895
BspFNI CGCG 2 cut(s) 660, 780
BspLI GGNNCC 2 cut(s) 589, 749
BspPI GGATC 2 cut(s) 744, 1040
BspQI GCTCTTC 1 cut(s) 485
BspT104I TTCGAA 2 cut(s) 306, 313
BspT107I GGYRCC 2 cut(s) 587, 747
BsrFI RCCGGY 3 cut(s) 468, 590, 1168
BsrGI TGTACA 1 cut(s) 447
BsrI ACTGG 3 cut(s) 368, 835, 861
BssAI RCCGGY 3 cut(s) 468, 590, 1168
BssMI GATC 6 cut(s) 519, 736, 771, 801, 1045, 1089
BssNAI GTATAC 1 cut(s) 872
Bst1107I GTATAC 1 cut(s) 872
Bst2UI CCWGG 1 cut(s) 687
Bst4CI ACNGT 5 cut(s) 322, 387, 683, 902, 954
Bst6I CTCTTC 2 cut(s) 412, 485
BstAUI TGTACA 1 cut(s) 447
BstBI TTCGAA 2 cut(s) 306, 313
BstC8I GCNNGC 2 cut(s) 474, 1061
BstDEI CTNAG 7 cut(s) 81, 125, 405, 426, 598, 752, 882
BstF5I GGATG 2 cut(s) 565, 634
BstFNI CGCG 2 cut(s) 660, 780
BstHHI GCGC 1 cut(s) 782
BstKTI GATC 6 cut(s) 522, 739, 774, 804, 1048, 1092
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 6 cut(s) 519, 736, 771, 801, 1045, 1089
BstMWI GCNNNNNNNGC 1 cut(s) 594
BstNI CCWGG 1 cut(s) 687
BstSCI CCNGG 2 cut(s) 540, 685
BstSFI CTRYAG 1 cut(s) 318
BstSLI GKGCMC 1 cut(s) 590
BstUI CGCG 2 cut(s) 660, 780
BstV1I GCAGC 2 cut(s) 61, 872
BstX2I RGATCY 2 cut(s) 736, 1045
BstYI RGATCY 2 cut(s) 736, 1045
BstZ17I GTATAC 1 cut(s) 872
Bsu36I CCTNAGG 1 cut(s) 752
BsuRI GGCC 1 cut(s) 1059
BtsCI GGATG 2 cut(s) 565, 634
BtsIMutI CAGTG 3 cut(s) 327, 890, 908
Cac8I GCNNGC 2 cut(s) 474, 1061
CaiI CAGNNNCTG 2 cut(s) 326, 1049
CfoI GCGC 1 cut(s) 782
Cfr10I RCCGGY 3 cut(s) 468, 590, 1168
Cfr13I GGNCC 2 cut(s) 651, 683
CpoI CGGWCCG 1 cut(s) 651
Csp6I GTAC 4 cut(s) 448, 703, 748, 950
CspAI ACCGGT 2 cut(s) 590, 1168
CspI CGGWCCG 1 cut(s) 651
CviAII CATG 2 cut(s) 250, 842
CviQI GTAC 4 cut(s) 448, 703, 748, 950
DdeI CTNAG 7 cut(s) 81, 125, 405, 426, 598, 752, 882
DpnI GATC 6 cut(s) 521, 738, 773, 803, 1047, 1091
DpnII GATC 6 cut(s) 519, 736, 771, 801, 1045, 1089
EaeI YGGCCR 1 cut(s) 1057
Eam1104I CTCTTC 2 cut(s) 412, 485
EarI CTCTTC 2 cut(s) 412, 485
Eco47I GGWCC 2 cut(s) 651, 683
Eco57I CTGAAG 2 cut(s) 212, 545
Eco81I CCTNAGG 1 cut(s) 752
EcoRII CCWGG 1 cut(s) 685
FaeI CATG 2 cut(s) 253, 845
FatI CATG 2 cut(s) 249, 841
FbaI TGATCA 2 cut(s) 771, 1089
FblI GTMKAC 1 cut(s) 871
Fnu4HI GCNGC 5 cut(s) 75, 78, 661, 664, 861
FokI GGATG 2 cut(s) 552, 641
Fsp4HI GCNGC 5 cut(s) 75, 78, 661, 664, 861
FspBI CTAG 2 cut(s) 339, 458
GlaI GCGC 1 cut(s) 781
GluI GCNGC 5 cut(s) 75, 78, 661, 664, 861
GsuI CTGGAG 1 cut(s) 298
HaeIII GGCC 1 cut(s) 1059
HapII CCGG 5 cut(s) 469, 542, 591, 649, 1169
HhaI GCGC 1 cut(s) 782
Hin1II CATG 2 cut(s) 253, 845
Hin6I GCGC 1 cut(s) 780
HinP1I GCGC 1 cut(s) 780
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 474
HinfI GANTC 9 cut(s) 342, 363, 410, 422, 529, 545, 715, 1010, 1122
HpaII CCGG 5 cut(s) 469, 542, 591, 649, 1169
HphI GGTGA 4 cut(s) 154, 246, 638, 781
Hpy166II GTNNAC 5 cut(s) 46, 646, 872, 962, 1166
Hpy188I TCNGA 8 cut(s) 151, 358, 427, 519, 601, 693, 722, 1105
Hpy8I GTNNAC 5 cut(s) 46, 646, 872, 962, 1166
Hpy99I CGWCG 1 cut(s) 1147
HpyCH4III ACNGT 5 cut(s) 322, 387, 683, 902, 954
HpyCH4IV ACGT 2 cut(s) 915, 1145
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 594
HpyF3I CTNAG 7 cut(s) 81, 125, 405, 426, 598, 752, 882
HpySE526I ACGT 2 cut(s) 915, 1145
Hsp92II CATG 2 cut(s) 253, 845
HspAI GCGC 1 cut(s) 780
KpnI GGTACC 1 cut(s) 751
Ksp22I TGATCA 2 cut(s) 771, 1089
Kzo9I GATC 6 cut(s) 519, 736, 771, 801, 1045, 1089
LguI GCTCTTC 1 cut(s) 485
LmnI GCTCC 2 cut(s) 454, 637
Lsp1109I GCAGC 2 cut(s) 61, 872
LweI GCATC 4 cut(s) 94, 574, 619, 818
MaeI CTAG 2 cut(s) 339, 458
MaeII ACGT 2 cut(s) 915, 1145
MaeIII GTNAC 7 cut(s) 160, 210, 252, 322, 670, 756, 916
MalI GATC 6 cut(s) 521, 738, 773, 803, 1047, 1091
MboI GATC 6 cut(s) 519, 736, 771, 801, 1045, 1089
MboII GAAGA 7 cut(s) 83, 292, 295, 429, 502, 551, 731
MflI RGATCY 2 cut(s) 736, 1045
MhlI GDGCHC 1 cut(s) 590
MlsI TGGCCA 1 cut(s) 1059
MluCI AATT 8 cut(s) 39, 177, 181, 265, 613, 1126, 1190, 1194
MluNI TGGCCA 1 cut(s) 1059
MlyI GAGTC 3 cut(s) 431, 539, 1019
MmeI TCCRAC 4 cut(s) 422, 923, 958, 1099
Mox20I TGGCCA 1 cut(s) 1059
MroXI GAANNNNTTC 1 cut(s) 1110
MscI TGGCCA 1 cut(s) 1059
MseI TTAA 5 cut(s) 180, 375, 827, 929, 1193
Msp20I TGGCCA 1 cut(s) 1059
MspI CCGG 5 cut(s) 469, 542, 591, 649, 1169
MspR9I CCNGG 2 cut(s) 542, 687
MvaI CCWGG 1 cut(s) 687
MvnI CGCG 2 cut(s) 660, 780
MwoI GCNNNNNNNGC 1 cut(s) 594
NciI CCSGG 1 cut(s) 542
NdeII GATC 6 cut(s) 519, 736, 771, 801, 1045, 1089
NlaIII CATG 2 cut(s) 253, 845
NlaIV GGNNCC 2 cut(s) 589, 749
NmuCI GTSAC 4 cut(s) 160, 252, 322, 756
NspV TTCGAA 2 cut(s) 306, 313
PciSI GCTCTTC 1 cut(s) 485
PcsI WCGNNNNNNNCGW 1 cut(s) 206
PdmI GAANNNNTTC 1 cut(s) 1110
PfeI GAWTC 6 cut(s) 342, 363, 410, 529, 715, 1122
PflMI CCANNNNNTGG 1 cut(s) 856
PinAI ACCGGT 2 cut(s) 590, 1168
PkrI GCNGC 5 cut(s) 76, 79, 662, 665, 862
PleI GAGTC 3 cut(s) 430, 539, 1018
PpsI GAGTC 3 cut(s) 430, 539, 1018
PshBI ATTAAT 2 cut(s) 180, 1193
PsiI TTATAA 1 cut(s) 1131
Psp6I CCWGG 1 cut(s) 685
PspGI CCWGG 1 cut(s) 685
PspN4I GGNNCC 2 cut(s) 589, 749
PspPI GGNCC 2 cut(s) 651, 683
PstNI CAGNNNCTG 2 cut(s) 326, 1049
PsuI RGATCY 2 cut(s) 736, 1045
RsaI GTAC 4 cut(s) 449, 704, 749, 951
RsaNI GTAC 4 cut(s) 448, 703, 748, 950
Rsr2I CGGWCCG 1 cut(s) 651
RsrII CGGWCCG 1 cut(s) 651
SapI GCTCTTC 1 cut(s) 485
SaqAI TTAA 5 cut(s) 180, 375, 827, 929, 1193
SatI GCNGC 5 cut(s) 75, 78, 661, 664, 861
Sau3AI GATC 6 cut(s) 519, 736, 771, 801, 1045, 1089
Sau96I GGNCC 2 cut(s) 651, 683
SchI GAGTC 3 cut(s) 431, 539, 1019
ScrFI CCNGG 2 cut(s) 542, 687
SduI GDGCHC 1 cut(s) 590
SfaNI GCATC 4 cut(s) 94, 574, 619, 818
SfcI CTRYAG 1 cut(s) 318
SfuI TTCGAA 2 cut(s) 306, 313
SinI GGWCC 2 cut(s) 651, 683
SmlI CTYRAG 3 cut(s) 623, 761, 818
SmoI CTYRAG 3 cut(s) 623, 761, 818
Sse9I AATT 8 cut(s) 39, 177, 181, 265, 613, 1126, 1190, 1194
SsiI CCGC 3 cut(s) 77, 660, 663
SspMI CTAG 2 cut(s) 339, 458
StyD4I CCNGG 2 cut(s) 540, 685
TaaI ACNGT 5 cut(s) 322, 387, 683, 902, 954
TaiI ACGT 2 cut(s) 918, 1148
TaqII GACCGA 1 cut(s) 668
TasI AATT 8 cut(s) 39, 177, 181, 265, 613, 1126, 1190, 1194
TatI WGTACW 1 cut(s) 447
TauI GCSGC 3 cut(s) 80, 663, 666
TfiI GAWTC 6 cut(s) 342, 363, 410, 529, 715, 1122
Tru1I TTAA 5 cut(s) 180, 375, 827, 929, 1193
Tru9I TTAA 5 cut(s) 180, 375, 827, 929, 1193
TscAI CASTG 3 cut(s) 327, 890, 915
TseFI GTSAC 4 cut(s) 160, 252, 322, 756
TseI GCWGC 2 cut(s) 74, 860
Tsp45I GTSAC 4 cut(s) 160, 252, 322, 756
TspDTI ATGAA 3 cut(s) 38, 728, 731
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 3 cut(s) 327, 890, 915
Van91I CCANNNNNTGG 1 cut(s) 856
VpaK11BI GGWCC 2 cut(s) 651, 683
VspI ATTAAT 2 cut(s) 180, 1193
XmiI GTMKAC 1 cut(s) 871
XmnI GAANNNNTTC 1 cut(s) 1110
XspI CTAG 2 cut(s) 339, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.