RLG00000011867

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005677
Physical Location & Seq
Forward (+)
13428566 .. 13432830
4265 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011867

Sequence Viewer

Length: 1608 bp
ATGTCTCATCACCAAAAGGAGGAATTAAGGGCAAACGTGGCTCTCACAGTTTTTGAACCAGACAAGCAGAAAGCCGTTCCCATTAAAGAATTGAAAGAGACACTTCTGAGGCTTGAGGAACAGAAGAAAGAGCTGGAGAAAAAGGTTCGTGCACAAAACCAGAAGGCTCAAAAATGTTCTACAGCAGCAGAGACTGAGAAGGCTCTACAAATTATTACTAGAGCATTAAATCTATATGTCTTAAAGAAATGCATGAAGAAGAAGGGGCGCTTACAACAGAAAATCGATAAGCTTGAAGCTTCCAACTTCGGCATTGAGATTCAGATTACTTTACTCGAAGGACATGCGAATATTGGGAAAACACCTGTTTATGATGACTCACCAACAAAAGGAGAATCTGCAATGAAATCTAAGGACCTGGACATGATGAACAATGATGAATTGAGGGAAATGATAGATGAGATCATTAAGGACGTTGAATACATGCAATTGAAATCCTCAGGAAAATTATGGAGGCGTTGTAGGCACACACTGAACAAACCATTTGTTTCGATGAGGAATAATCTCTGCAAGTTGGTTCAAGAACGCCTGAAAGCTTTGAACGCACCGCCTAGCACCCCCATTCCTATTCCTCCATCTTTGAAAGATGATCTTTCGATACAAACCAAGAGCTACTCCTCCATCTCCACTAATCGAAAACCAGATTCCCTAGCTACATATTGCTTCTTTTCACAAATCTCTGTTGTTCCCAAACCTCCTCGGTGCCTCTCCCAGAGAATCCAATCGAAATCAGACAGAATCCCAGTTGTTAAAACCCAACAGTCGAGCTTTCCAACTACTCAGGAATCGAGAAGAGAGTCTGACTGTGAGTGTTTCAATCTATACAAGGAGCTAGTTCCTTACAGGCAAGCTTGGTCTTGGCAGAAGAGCATTGTCAAGGAGAAAAAGGCTCTGATTGACAGGAACGAGGACTGCCCGGACTCGCTCTTTGTTCTTCAGCATCCTCCTGTTTATACAATGGGCACCGGTAGCTCAGAAAAGTTCCTCAATTTTGACCTCAAGGATGCTCCATTTGATGTTCACCGGACCGAACGCGGCGGCGAGGTTACATATCATGGTCCTGGTCAGATAGTAATGTACCCCATTATGAATCTCCGAAATCACAAAATGGATCTTCATTGGTACCTCAGGTCACTTGAGGAGGTGATCATTCGCGCTCTTTCAAAAACATTTTCGATCAAGGCATCTCGGCTTGAGGGCTTAACTGGTGTTTGGCATGGAAACCAGAAACTGGCAGCCATTGGTATACGAGTGTCTCAGTGGATAGCTTATCACGGTTTGGCACTGAACGTAACCACAGATTTAACTCCTTTTCGTTGGATAGTACCGTGTGGTTTACAAAACTATCAGGTCGGAAGCATTAGAGGGTTGTTAGGGGAGTTTGAGTCATTTGACGATAGTAGTAGAAAACAGCTACCAGATCCTGATGATGGCCAGCTACTTAATATTGCTTGTAAATCTTTGATCAATGAGTTTTCAGAAGTTTTTCAGGTTGGAATCAATTATGAAACCATCTCGAGGTTAGAGTTTTTAGAAGTGAAACTGTAA

Protein Analysis

536

Amino Acids

61.29

Weight (kDa)

9.04

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 301 - 484 1.1e-39 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1182
AccB1I GGYRCC 3 cut(s) 762, 1022, 1182
AccB7I CCANNNNNTGG 1 cut(s) 1291
AccI GTMKAC 1 cut(s) 1306
AccII CGCG 2 cut(s) 1095, 1215
AciI CCGC 3 cut(s) 608, 1095, 1098
AclWI GGATC 2 cut(s) 1179, 1475
AcoI YGGCCR 1 cut(s) 1492
AcuI CTGAAG 1 cut(s) 980
AfaI GTAC 3 cut(s) 1139, 1184, 1386
AfiI CCNNNNNNNGG 5 cut(s) 19, 389, 1291, 1490, 1578
AgeI ACCGGT 1 cut(s) 1025
AjnI CCWGG 2 cut(s) 417, 1120
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 4 cut(s) 9, 92, 185, 1320
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 2 cut(s) 1179, 1475
AlwNI CAGNNNCTG 3 cut(s) 194, 1291, 1484
Ama87I CYCGRG 1 cut(s) 1576
AoxI GGCC 1 cut(s) 1492
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 185, 1295
ArsI GACNNNNNNTTYG 2 cut(s) 971, 1003
AsiGI ACCGGT 1 cut(s) 1025
Asp700I GAANNNNTTC 1 cut(s) 1545
Asp718I GGTACC 1 cut(s) 1182
AspLEI GCGC 2 cut(s) 270, 1217
AspS9I GGNCC 3 cut(s) 415, 1086, 1118
AsuC2I CCSGG 1 cut(s) 977
AsuHPI GGTGA 3 cut(s) 372, 1073, 1216
AvaI CYCGRG 1 cut(s) 1576
AvaII GGWCC 3 cut(s) 415, 1086, 1118
AxyI CCTNAGG 2 cut(s) 499, 1187
BaeGI GKGCMC 2 cut(s) 154, 1025
BalI TGGCCA 1 cut(s) 1494
BanI GGYRCC 3 cut(s) 762, 1022, 1182
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 2 cut(s) 197, 1307
BccI CCATC 4 cut(s) 643, 689, 1484, 1580
BceAI ACGGC 1 cut(s) 59
BcgI CGANNNNNNTGC 2 cut(s) 326, 360
BciT130I CCWGG 2 cut(s) 419, 1122
BclI TGATCA 2 cut(s) 1206, 1524
BcnI CCSGG 1 cut(s) 977
BcoDI GTCTC 4 cut(s) 9, 92, 185, 1320
BfaI CTAG 4 cut(s) 219, 612, 710, 893
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 1 cut(s) 271
BisI GCNGC 4 cut(s) 186, 1096, 1099, 1296
BlsI GCNGC 4 cut(s) 187, 1097, 1100, 1297
Bme1390I CCNGG 3 cut(s) 419, 977, 1122
Bme18I GGWCC 3 cut(s) 415, 1086, 1118
BmeT110I CYCGRG 1 cut(s) 1576
BmgT120I GGNCC 3 cut(s) 415, 1086, 1118
BmiI GGNNCC 3 cut(s) 764, 1024, 1184
BmrFI CCNGG 3 cut(s) 419, 977, 1122
BmrI ACTGGG 1 cut(s) 797
BmsI GCATC 3 cut(s) 1009, 1054, 1253
BmuI ACTGGG 1 cut(s) 797
BpmI CTGGAG 1 cut(s) 155
BpuEI CTTGAG 4 cut(s) 134, 1043, 1217, 1274
BpuMI CCSGG 1 cut(s) 977
Bsa29I ATCGAT 1 cut(s) 285
BsaJI CCNNGG 1 cut(s) 758
BsaWI WCCGGW 2 cut(s) 1025, 1083
BsaXI ACNNNNNCTCC 4 cut(s) 505, 535, 1430, 1460
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 389, 1291, 1490, 1578
Bse118I RCCGGY 1 cut(s) 1025
Bse1I ACTGG 3 cut(s) 803, 1270, 1296
Bse21I CCTNAGG 2 cut(s) 499, 1187
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 2 cut(s) 419, 1122
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 758
BseGI GGATG 2 cut(s) 1000, 1069
BseLI CCNNNNNNNGG 5 cut(s) 19, 389, 1291, 1490, 1578
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 7 cut(s) 98, 186, 513, 854, 1047, 1201, 1331
BseNI ACTGG 3 cut(s) 803, 1270, 1296
BseRI GAGGAG 3 cut(s) 667, 747, 1214
BseSI GKGCMC 2 cut(s) 154, 1025
BseXI GCAGC 2 cut(s) 197, 1307
Bsh1236I CGCG 2 cut(s) 1095, 1215
BshFI GGCC 1 cut(s) 1494
BshNI GGYRCC 3 cut(s) 762, 1022, 1182
BshTI ACCGGT 1 cut(s) 1025
BshVI ATCGAT 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 154
BsiHKCI CYCGRG 1 cut(s) 1576
BsiSI CCGG 3 cut(s) 977, 1026, 1084
BslI CCNNNNNNNGG 5 cut(s) 19, 389, 1291, 1490, 1578
BsmAI GTCTC 4 cut(s) 9, 92, 185, 1320
BsnI GGCC 1 cut(s) 1494
BsoBI CYCGRG 1 cut(s) 1576
Bsp1286I GDGCHC 2 cut(s) 154, 1025
Bsp143I GATC 7 cut(s) 462, 649, 1171, 1206, 1236, 1480, 1524
BspACI CCGC 3 cut(s) 608, 1095, 1098
BspANI GGCC 1 cut(s) 1494
BspCNI CTCAG 7 cut(s) 99, 187, 512, 853, 1046, 1200, 1330
BspDI ATCGAT 1 cut(s) 285
BspFNI CGCG 2 cut(s) 1095, 1215
BspLI GGNNCC 3 cut(s) 764, 1024, 1184
BspPI GGATC 2 cut(s) 1179, 1475
BspQI GCTCTTC 1 cut(s) 920
BspT107I GGYRCC 3 cut(s) 762, 1022, 1182
BsrDI GCAATG 1 cut(s) 408
BsrFI RCCGGY 1 cut(s) 1025
BsrI ACTGG 3 cut(s) 803, 1270, 1296
BssAI RCCGGY 1 cut(s) 1025
BssECI CCNNGG 1 cut(s) 758
BssMI GATC 7 cut(s) 462, 649, 1171, 1206, 1236, 1480, 1524
BssNAI GTATAC 1 cut(s) 1307
Bst1107I GTATAC 1 cut(s) 1307
Bst2UI CCWGG 2 cut(s) 419, 1122
Bst4CI ACNGT 6 cut(s) 49, 822, 866, 1337, 1389, 1605
Bst6I CTCTTC 2 cut(s) 847, 920
BstC8I GCNNGC 2 cut(s) 909, 1496
BstDEI CTNAG 8 cut(s) 107, 195, 411, 499, 840, 1033, 1187, 1317
BstF5I GGATG 2 cut(s) 1000, 1069
BstFNI CGCG 2 cut(s) 1095, 1215
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 2 cut(s) 270, 1217
BstKTI GATC 7 cut(s) 465, 652, 1174, 1209, 1239, 1483, 1527
BstMAI GTCTC 4 cut(s) 9, 92, 185, 1320
BstMBI GATC 7 cut(s) 462, 649, 1171, 1206, 1236, 1480, 1524
BstMWI GCNNNNNNNGC 4 cut(s) 38, 523, 602, 1029
BstNI CCWGG 2 cut(s) 419, 1122
BstNSI RCATGY 2 cut(s) 347, 487
BstSCI CCNGG 3 cut(s) 417, 975, 1120
BstSFI CTRYAG 1 cut(s) 180
BstSLI GKGCMC 2 cut(s) 154, 1025
BstUI CGCG 2 cut(s) 1095, 1215
BstV1I GCAGC 2 cut(s) 197, 1307
BstX2I RGATCY 2 cut(s) 1171, 1480
BstYI RGATCY 2 cut(s) 1171, 1480
BstZ17I GTATAC 1 cut(s) 1307
Bsu15I ATCGAT 1 cut(s) 285
Bsu36I CCTNAGG 2 cut(s) 499, 1187
BsuRI GGCC 1 cut(s) 1494
BsuTUI ATCGAT 1 cut(s) 285
BtsCI GGATG 2 cut(s) 1000, 1069
BtsIMutI CAGTG 3 cut(s) 530, 1325, 1343
Cac8I GCNNGC 2 cut(s) 909, 1496
CaiI CAGNNNCTG 3 cut(s) 194, 1291, 1484
CfoI GCGC 2 cut(s) 270, 1217
Cfr10I RCCGGY 1 cut(s) 1025
Cfr13I GGNCC 3 cut(s) 415, 1086, 1118
ClaI ATCGAT 1 cut(s) 285
CpoI CGGWCCG 1 cut(s) 1086
Csp6I GTAC 3 cut(s) 1138, 1183, 1385
CspAI ACCGGT 1 cut(s) 1025
CspI CGGWCCG 1 cut(s) 1086
CviAII CATG 6 cut(s) 253, 344, 424, 484, 1115, 1277
CviQI GTAC 3 cut(s) 1138, 1183, 1385
DdeI CTNAG 8 cut(s) 107, 195, 411, 499, 840, 1033, 1187, 1317
DpnI GATC 7 cut(s) 464, 651, 1173, 1208, 1238, 1482, 1526
DpnII GATC 7 cut(s) 462, 649, 1171, 1206, 1236, 1480, 1524
EaeI YGGCCR 1 cut(s) 1492
Eam1104I CTCTTC 2 cut(s) 847, 920
EarI CTCTTC 2 cut(s) 847, 920
Eco47I GGWCC 3 cut(s) 415, 1086, 1118
Eco57I CTGAAG 1 cut(s) 980
Eco81I CCTNAGG 2 cut(s) 499, 1187
Eco88I CYCGRG 1 cut(s) 1576
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 417, 1120
EcoT22I ATGCAT 1 cut(s) 254
FaeI CATG 6 cut(s) 256, 347, 427, 487, 1118, 1280
FalI AAGNNNNNCTT 6 cut(s) 87, 119, 254, 286, 636, 668
FatI CATG 6 cut(s) 252, 343, 423, 483, 1114, 1276
FbaI TGATCA 2 cut(s) 1206, 1524
FblI GTMKAC 1 cut(s) 1306
Fnu4HI GCNGC 4 cut(s) 186, 1096, 1099, 1296
FokI GGATG 2 cut(s) 987, 1076
Fsp4HI GCNGC 4 cut(s) 186, 1096, 1099, 1296
FspBI CTAG 4 cut(s) 219, 612, 710, 893
GlaI GCGC 2 cut(s) 269, 1216
GluI GCNGC 4 cut(s) 186, 1096, 1099, 1296
GsuI CTGGAG 1 cut(s) 155
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 1 cut(s) 1494
HapII CCGG 3 cut(s) 977, 1026, 1084
HhaI GCGC 2 cut(s) 270, 1217
Hin1II CATG 6 cut(s) 256, 347, 427, 487, 1118, 1280
Hin6I GCGC 2 cut(s) 268, 1215
HinP1I GCGC 2 cut(s) 268, 1215
HindIII AAGCTT 4 cut(s) 290, 297, 594, 909
HpaII CCGG 3 cut(s) 977, 1026, 1084
HphI GGTGA 3 cut(s) 372, 1073, 1216
Hpy166II GTNNAC 4 cut(s) 152, 1081, 1307, 1397
Hpy188III TCNNGA 6 cut(s) 501, 581, 842, 849, 1484, 1576
Hpy8I GTNNAC 4 cut(s) 152, 1081, 1307, 1397
HpyAV CCTTC 4 cut(s) 157, 193, 256, 332
HpyCH4III ACNGT 6 cut(s) 49, 822, 866, 1337, 1389, 1605
HpyCH4IV ACGT 3 cut(s) 36, 474, 1350
HpyCH4V TGCA 5 cut(s) 152, 252, 401, 487, 570
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 523, 602, 1029
HpyF3I CTNAG 8 cut(s) 107, 195, 411, 499, 840, 1033, 1187, 1317
HpySE526I ACGT 3 cut(s) 36, 474, 1350
Hsp92II CATG 6 cut(s) 256, 347, 427, 487, 1118, 1280
HspAI GCGC 2 cut(s) 268, 1215
KpnI GGTACC 1 cut(s) 1186
Ksp22I TGATCA 2 cut(s) 1206, 1524
Kzo9I GATC 7 cut(s) 462, 649, 1171, 1206, 1236, 1480, 1524
LguI GCTCTTC 1 cut(s) 920
LmnI GCTCC 2 cut(s) 889, 1072
Lsp1109I GCAGC 2 cut(s) 197, 1307
LweI GCATC 3 cut(s) 1009, 1054, 1253
MaeI CTAG 4 cut(s) 219, 612, 710, 893
MaeII ACGT 3 cut(s) 36, 474, 1350
MaeIII GTNAC 3 cut(s) 1105, 1191, 1351
MalI GATC 7 cut(s) 464, 651, 1173, 1208, 1238, 1482, 1526
MboI GATC 7 cut(s) 462, 649, 1171, 1206, 1236, 1480, 1524
MboII GAAGA 7 cut(s) 136, 268, 271, 864, 937, 986, 1166
MfeI CAATTG 1 cut(s) 488
MflI RGATCY 2 cut(s) 1171, 1480
MhlI GDGCHC 2 cut(s) 154, 1025
MlsI TGGCCA 1 cut(s) 1494
MluCI AATT 8 cut(s) 23, 89, 210, 440, 488, 506, 1048, 1561
MluNI TGGCCA 1 cut(s) 1494
MlyI GAGTC 4 cut(s) 371, 866, 974, 1454
MmeI TCCRAC 5 cut(s) 327, 857, 1358, 1393, 1534
Mox20I TGGCCA 1 cut(s) 1494
Mph1103I ATGCAT 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 1545
MscI TGGCCA 1 cut(s) 1494
MseI TTAA 9 cut(s) 26, 84, 227, 242, 468, 810, 1262, 1364, 1503
Msp20I TGGCCA 1 cut(s) 1494
MspI CCGG 3 cut(s) 977, 1026, 1084
MspR9I CCNGG 3 cut(s) 419, 977, 1122
MunI CAATTG 1 cut(s) 488
MvaI CCWGG 2 cut(s) 419, 1122
MvnI CGCG 2 cut(s) 1095, 1215
MwoI GCNNNNNNNGC 4 cut(s) 38, 523, 602, 1029
NciI CCSGG 1 cut(s) 977
NdeII GATC 7 cut(s) 462, 649, 1171, 1206, 1236, 1480, 1524
NlaIII CATG 6 cut(s) 256, 347, 427, 487, 1118, 1280
NlaIV GGNNCC 3 cut(s) 764, 1024, 1184
NmeAIII GCCGAG 1 cut(s) 1228
NmuCI GTSAC 1 cut(s) 1191
NsiI ATGCAT 1 cut(s) 254
NspI RCATGY 2 cut(s) 347, 487
PaeR7I CTCGAG 1 cut(s) 1576
PciSI GCTCTTC 1 cut(s) 920
PdmI GAANNNNTTC 1 cut(s) 1545
PfeI GAWTC 8 cut(s) 319, 395, 704, 777, 798, 845, 1150, 1557
PflMI CCANNNNNTGG 1 cut(s) 1291
PinAI ACCGGT 1 cut(s) 1025
PkrI GCNGC 4 cut(s) 187, 1097, 1100, 1297
PleI GAGTC 4 cut(s) 371, 865, 974, 1453
PpsI GAGTC 4 cut(s) 371, 865, 974, 1453
PpuMI RGGWCCY 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 2 cut(s) 417, 1120
PspGI CCWGG 2 cut(s) 417, 1120
PspN4I GGNNCC 3 cut(s) 764, 1024, 1184
PspPI GGNCC 3 cut(s) 415, 1086, 1118
PspPPI RGGWCCY 1 cut(s) 415
PstNI CAGNNNCTG 3 cut(s) 194, 1291, 1484
PsuI RGATCY 2 cut(s) 1171, 1480
RsaI GTAC 3 cut(s) 1139, 1184, 1386
RsaNI GTAC 3 cut(s) 1138, 1183, 1385
Rsr2I CGGWCCG 1 cut(s) 1086
RsrII CGGWCCG 1 cut(s) 1086
SapI GCTCTTC 1 cut(s) 920
SaqAI TTAA 9 cut(s) 26, 84, 227, 242, 468, 810, 1262, 1364, 1503
SatI GCNGC 4 cut(s) 186, 1096, 1099, 1296
Sau3AI GATC 7 cut(s) 462, 649, 1171, 1206, 1236, 1480, 1524
Sau96I GGNCC 3 cut(s) 415, 1086, 1118
SchI GAGTC 4 cut(s) 371, 866, 974, 1454
ScrFI CCNGG 3 cut(s) 419, 977, 1122
SduI GDGCHC 2 cut(s) 154, 1025
SfaNI GCATC 3 cut(s) 1009, 1054, 1253
SfcI CTRYAG 1 cut(s) 180
Sfr274I CTCGAG 1 cut(s) 1576
SinI GGWCC 3 cut(s) 415, 1086, 1118
SlaI CTCGAG 1 cut(s) 1576
SmlI CTYRAG 5 cut(s) 113, 1058, 1196, 1253, 1576
SmoI CTYRAG 5 cut(s) 113, 1058, 1196, 1253, 1576
Sse9I AATT 8 cut(s) 23, 89, 210, 440, 488, 506, 1048, 1561
SsiI CCGC 3 cut(s) 608, 1095, 1098
SspI AATATT 2 cut(s) 352, 1507
SspMI CTAG 4 cut(s) 219, 612, 710, 893
StyD4I CCNGG 3 cut(s) 417, 975, 1120
TaaI ACNGT 6 cut(s) 49, 822, 866, 1337, 1389, 1605
TaiI ACGT 3 cut(s) 39, 477, 1353
TaqII GACCGA 1 cut(s) 1103
TasI AATT 8 cut(s) 23, 89, 210, 440, 488, 506, 1048, 1561
TauI GCSGC 2 cut(s) 1098, 1101
TfiI GAWTC 8 cut(s) 319, 395, 704, 777, 798, 845, 1150, 1557
Tru1I TTAA 9 cut(s) 26, 84, 227, 242, 468, 810, 1262, 1364, 1503
Tru9I TTAA 9 cut(s) 26, 84, 227, 242, 468, 810, 1262, 1364, 1503
TscAI CASTG 3 cut(s) 537, 1325, 1350
TseFI GTSAC 1 cut(s) 1191
TseI GCWGC 2 cut(s) 185, 1295
Tsp45I GTSAC 1 cut(s) 1191
TspDTI ATGAA 7 cut(s) 269, 419, 443, 453, 1163, 1166, 1581
TspRI CASTG 3 cut(s) 537, 1325, 1350
Van91I CCANNNNNTGG 1 cut(s) 1291
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 3 cut(s) 415, 1086, 1118
XceI RCATGY 2 cut(s) 347, 487
XhoI CTCGAG 1 cut(s) 1576
XmiI GTMKAC 1 cut(s) 1306
XmnI GAANNNNTTC 1 cut(s) 1545
XspI CTAG 4 cut(s) 219, 612, 710, 893
Zsp2I ATGCAT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.