Rw5G045220

Plastidial lipoyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005677
Physical Location & Seq
Forward (+)
79093723 .. 79094950
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045220.1

Sequence Viewer

Length: 942 bp
ATGACTTTCTTGGCGACCCAATGTGTTGTTGCCTCAATCCCAACATACCCAGTTTCTAATCATCAGCCCAAACGGTGCCTCTCCCAGAGAATCCAATCGGACAGAATCCCAGTTGTTAAAACCCAACAGTTGAGCTTTCCAACTACTTGGGAATCGAGAAGAGAGTCTGAGTGTGAGTGCTTCAATCTGTACAAGGAGCTAGTTCCTTACCGGCAAGCTTGGTCTTGGCAGAAGAGCATTGTCAAGGAGAAAAAGGCTCTGATTGAGAGGAATCAGGACTGCCCGGACTCGCTGTTTGTTCTTCAGCATCCTCCTGTTTATACAATGGGCACCGGTAGCTCAGAAAAGTTCCTCAATTTTGACCTCAAGGATGCTCCATTTGATGTTCATCGGACCGAACGCGGCGGCGAGGTTACATATCACGGTCCTGGTCAGATAGTAATGTACCCCATTATGAATCTCCGAAATCACAAAATGGATCTTCATTGGTACCTCAGGTCACTTGAGGAGGTGATCATTCGCGCTCTTTCAAAGACATTTTCAATCAAGGCATCTCGACTTGAGGGCTTAACTGGTGTTTGGCATGGAAACCAGAAACTGGCAGCCATTGGTATACGAGTGTCTCACTGGATAGCTTATCACGGTTTGGCACTGAACGTAACCACAGATTTAACTCCTTTTGGTTGGATAGTACCGTGTGGTTTACAAAACTATCAGGTTGGAAGCATTAGAGGGTTGTTGGGGGAGTTTGATTCATTTGACAATAGTAGTAGAAAACAGCTACCAGATCCTGATGATGGCCAGCTTCTTGATATTGCTTGTAAATCTTTGATCAATGAGTTTTCAGAAGTTTTTCAGGTTGGAATCAATTATGAAACCATCTCGAGGTTAGAGTTTTTAGAAAGTGAACTGGTAAAGCCTCTAAAAGAGGAAGTAATTTAG

Protein Analysis

313

Amino Acids

35.85

Weight (kDa)

6.51

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 70 - 249 5.9e-40 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 489
AccB1I GGYRCC 3 cut(s) 75, 329, 489
AccB7I CCANNNNNTGG 1 cut(s) 598
AccI GTMKAC 1 cut(s) 613
AccII CGCG 2 cut(s) 402, 522
AciI CCGC 2 cut(s) 402, 405
AclWI GGATC 2 cut(s) 486, 782
AcoI YGGCCR 1 cut(s) 799
AcuI CTGAAG 1 cut(s) 287
AfaI GTAC 4 cut(s) 191, 446, 491, 693
AfiI CCNNNNNNNGG 3 cut(s) 598, 797, 885
AgeI ACCGGT 1 cut(s) 332
AgsI TTSAA 3 cut(s) 184, 531, 543
AjnI CCWGG 1 cut(s) 427
AluBI AGCT 7 cut(s) 135, 199, 218, 339, 635, 781, 805
AluI AGCT 7 cut(s) 135, 199, 218, 339, 635, 781, 805
Alw26I GTCTC 1 cut(s) 627
AlwI GGATC 2 cut(s) 486, 782
AlwNI CAGNNNCTG 2 cut(s) 598, 791
Ama87I CYCGRG 1 cut(s) 883
AoxI GGCC 1 cut(s) 799
ApeKI GCWGC 1 cut(s) 602
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AsiGI ACCGGT 1 cut(s) 332
Asp700I GAANNNNTTC 1 cut(s) 852
Asp718I GGTACC 1 cut(s) 489
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 2 cut(s) 393, 425
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 883
AvaII GGWCC 2 cut(s) 393, 425
AxyI CCTNAGG 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 332
BalI TGGCCA 1 cut(s) 801
BanI GGYRCC 3 cut(s) 75, 329, 489
BbvI GCAGC 1 cut(s) 614
BccI CCATC 2 cut(s) 791, 887
BciT130I CCWGG 1 cut(s) 429
BclI TGATCA 2 cut(s) 513, 831
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 627
BfaI CTAG 1 cut(s) 200
BisI GCNGC 3 cut(s) 403, 406, 603
BlsI GCNGC 3 cut(s) 404, 407, 604
Bme1390I CCNGG 2 cut(s) 284, 429
Bme18I GGWCC 2 cut(s) 393, 425
BmeT110I CYCGRG 1 cut(s) 883
BmgT120I GGNCC 2 cut(s) 393, 425
BmiI GGNNCC 3 cut(s) 77, 331, 491
BmrFI CCNGG 2 cut(s) 284, 429
BmrI ACTGGG 2 cut(s) 44, 104
BmsI GCATC 3 cut(s) 316, 361, 560
BmuI ACTGGG 2 cut(s) 44, 104
BpuEI CTTGAG 3 cut(s) 350, 524, 581
BpuMI CCSGG 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 387
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 3 cut(s) 598, 797, 885
Bse118I RCCGGY 2 cut(s) 210, 332
Bse1I ACTGG 6 cut(s) 50, 110, 577, 603, 632, 915
Bse21I CCTNAGG 1 cut(s) 494
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 1 cut(s) 429
BseGI GGATG 2 cut(s) 307, 376
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 3 cut(s) 598, 797, 885
BseMII CTCAG 3 cut(s) 159, 354, 508
BseNI ACTGG 6 cut(s) 50, 110, 577, 603, 632, 915
BseRI GAGGAG 1 cut(s) 521
BseSI GKGCMC 1 cut(s) 332
BseXI GCAGC 1 cut(s) 614
Bsh1236I CGCG 2 cut(s) 402, 522
BshFI GGCC 1 cut(s) 801
BshNI GGYRCC 3 cut(s) 75, 329, 489
BshTI ACCGGT 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 883
BsiSI CCGG 3 cut(s) 211, 284, 333
BslI CCNNNNNNNGG 3 cut(s) 598, 797, 885
BsmAI GTCTC 1 cut(s) 627
BsnI GGCC 1 cut(s) 801
BsoBI CYCGRG 1 cut(s) 883
Bsp1286I GDGCHC 1 cut(s) 332
Bsp1407I TGTACA 1 cut(s) 189
Bsp143I GATC 4 cut(s) 478, 513, 787, 831
BspACI CCGC 2 cut(s) 402, 405
BspANI GGCC 1 cut(s) 801
BspCNI CTCAG 3 cut(s) 160, 353, 507
BspFNI CGCG 2 cut(s) 402, 522
BspLI GGNNCC 3 cut(s) 77, 331, 491
BspPI GGATC 2 cut(s) 486, 782
BspQI GCTCTTC 1 cut(s) 227
BspT107I GGYRCC 3 cut(s) 75, 329, 489
BsrFI RCCGGY 2 cut(s) 210, 332
BsrGI TGTACA 1 cut(s) 189
BsrI ACTGG 6 cut(s) 50, 110, 577, 603, 632, 915
BssAI RCCGGY 2 cut(s) 210, 332
BssMI GATC 4 cut(s) 478, 513, 787, 831
BssNAI GTATAC 1 cut(s) 614
Bst1107I GTATAC 1 cut(s) 614
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 5 cut(s) 75, 129, 425, 644, 696
Bst6I CTCTTC 2 cut(s) 154, 227
BstAUI TGTACA 1 cut(s) 189
BstC8I GCNNGC 2 cut(s) 216, 803
BstDEI CTNAG 3 cut(s) 168, 340, 494
BstF5I GGATG 2 cut(s) 307, 376
BstFNI CGCG 2 cut(s) 402, 522
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 4 cut(s) 481, 516, 790, 834
BstMAI GTCTC 1 cut(s) 627
BstMBI GATC 4 cut(s) 478, 513, 787, 831
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstNI CCWGG 1 cut(s) 429
BstSCI CCNGG 2 cut(s) 282, 427
BstSLI GKGCMC 1 cut(s) 332
BstUI CGCG 2 cut(s) 402, 522
BstV1I GCAGC 1 cut(s) 614
BstX2I RGATCY 2 cut(s) 478, 787
BstXI CCANNNNNNTGG 1 cut(s) 147
BstYI RGATCY 2 cut(s) 478, 787
BstZ17I GTATAC 1 cut(s) 614
Bsu36I CCTNAGG 1 cut(s) 494
BsuRI GGCC 1 cut(s) 801
BtsCI GGATG 2 cut(s) 307, 376
BtsIMutI CAGTG 2 cut(s) 625, 650
Cac8I GCNNGC 2 cut(s) 216, 803
CaiI CAGNNNCTG 2 cut(s) 598, 791
CfoI GCGC 1 cut(s) 524
Cfr10I RCCGGY 2 cut(s) 210, 332
Cfr13I GGNCC 2 cut(s) 393, 425
CpoI CGGWCCG 1 cut(s) 393
Csp6I GTAC 4 cut(s) 190, 445, 490, 692
CspAI ACCGGT 1 cut(s) 332
CspI CGGWCCG 1 cut(s) 393
CviAII CATG 1 cut(s) 584
CviQI GTAC 4 cut(s) 190, 445, 490, 692
DdeI CTNAG 3 cut(s) 168, 340, 494
DpnI GATC 4 cut(s) 480, 515, 789, 833
DpnII GATC 4 cut(s) 478, 513, 787, 831
EaeI YGGCCR 1 cut(s) 799
Eam1104I CTCTTC 2 cut(s) 154, 227
EarI CTCTTC 2 cut(s) 154, 227
Eco47I GGWCC 2 cut(s) 393, 425
Eco57I CTGAAG 1 cut(s) 287
Eco81I CCTNAGG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 883
EcoRII CCWGG 1 cut(s) 427
FaeI CATG 1 cut(s) 587
FaiI YATR 7 cut(s) 46, 321, 418, 455, 585, 614, 873
FatI CATG 1 cut(s) 583
FbaI TGATCA 2 cut(s) 513, 831
FblI GTMKAC 1 cut(s) 613
Fnu4HI GCNGC 3 cut(s) 403, 406, 603
FokI GGATG 2 cut(s) 294, 383
Fsp4HI GCNGC 3 cut(s) 403, 406, 603
FspBI CTAG 1 cut(s) 200
GlaI GCGC 1 cut(s) 523
GluI GCNGC 3 cut(s) 403, 406, 603
HaeIII GGCC 1 cut(s) 801
HapII CCGG 3 cut(s) 211, 284, 333
HhaI GCGC 1 cut(s) 524
Hin1II CATG 1 cut(s) 587
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 9 cut(s) 90, 105, 152, 164, 271, 287, 457, 752, 864
HpaII CCGG 3 cut(s) 211, 284, 333
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 3 cut(s) 614, 704, 908
Hpy188I TCNGA 8 cut(s) 100, 169, 261, 343, 393, 435, 464, 847
Hpy188III TCNNGA 6 cut(s) 156, 275, 555, 791, 809, 883
Hpy8I GTNNAC 3 cut(s) 614, 704, 908
HpyCH4III ACNGT 5 cut(s) 75, 129, 425, 644, 696
HpyCH4IV ACGT 1 cut(s) 657
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 3 cut(s) 168, 340, 494
HpySE526I ACGT 1 cut(s) 657
Hsp92II CATG 1 cut(s) 587
HspAI GCGC 1 cut(s) 522
KpnI GGTACC 1 cut(s) 493
Ksp22I TGATCA 2 cut(s) 513, 831
Kzo9I GATC 4 cut(s) 478, 513, 787, 831
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 2 cut(s) 196, 379
Lsp1109I GCAGC 1 cut(s) 614
LweI GCATC 3 cut(s) 316, 361, 560
MaeI CTAG 1 cut(s) 200
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 3 cut(s) 412, 498, 658
MalI GATC 4 cut(s) 480, 515, 789, 833
MboI GATC 4 cut(s) 478, 513, 787, 831
MboII GAAGA 4 cut(s) 171, 244, 293, 473
MflI RGATCY 2 cut(s) 478, 787
MhlI GDGCHC 1 cut(s) 332
MlsI TGGCCA 1 cut(s) 801
MluCI AATT 3 cut(s) 355, 868, 936
MluNI TGGCCA 1 cut(s) 801
MlyI GAGTC 2 cut(s) 173, 281
MmeI TCCRAC 4 cut(s) 164, 665, 700, 841
Mox20I TGGCCA 1 cut(s) 801
MroXI GAANNNNTTC 1 cut(s) 852
MscI TGGCCA 1 cut(s) 801
MseI TTAA 3 cut(s) 117, 569, 671
Msp20I TGGCCA 1 cut(s) 801
MspI CCGG 3 cut(s) 211, 284, 333
MspR9I CCNGG 2 cut(s) 284, 429
MvaI CCWGG 1 cut(s) 429
MvnI CGCG 2 cut(s) 402, 522
MwoI GCNNNNNNNGC 1 cut(s) 336
NciI CCSGG 1 cut(s) 284
NdeII GATC 4 cut(s) 478, 513, 787, 831
NlaIII CATG 1 cut(s) 587
NlaIV GGNNCC 3 cut(s) 77, 331, 491
NmuCI GTSAC 1 cut(s) 498
PaeR7I CTCGAG 1 cut(s) 883
PciSI GCTCTTC 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 852
PfeI GAWTC 7 cut(s) 90, 105, 152, 271, 457, 752, 864
PflMI CCANNNNNTGG 1 cut(s) 598
PinAI ACCGGT 1 cut(s) 332
PkrI GCNGC 3 cut(s) 404, 407, 604
PleI GAGTC 2 cut(s) 172, 281
PpsI GAGTC 2 cut(s) 172, 281
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 3 cut(s) 77, 331, 491
PspPI GGNCC 2 cut(s) 393, 425
PstNI CAGNNNCTG 2 cut(s) 598, 791
PsuI RGATCY 2 cut(s) 478, 787
RsaI GTAC 4 cut(s) 191, 446, 491, 693
RsaNI GTAC 4 cut(s) 190, 445, 490, 692
Rsr2I CGGWCCG 1 cut(s) 393
RsrII CGGWCCG 1 cut(s) 393
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 3 cut(s) 117, 569, 671
SatI GCNGC 3 cut(s) 403, 406, 603
Sau3AI GATC 4 cut(s) 478, 513, 787, 831
Sau96I GGNCC 2 cut(s) 393, 425
SchI GAGTC 2 cut(s) 173, 281
ScrFI CCNGG 2 cut(s) 284, 429
SduI GDGCHC 1 cut(s) 332
SfaNI GCATC 3 cut(s) 316, 361, 560
Sfr274I CTCGAG 1 cut(s) 883
SinI GGWCC 2 cut(s) 393, 425
SlaI CTCGAG 1 cut(s) 883
SmlI CTYRAG 4 cut(s) 365, 503, 560, 883
SmoI CTYRAG 4 cut(s) 365, 503, 560, 883
Sse9I AATT 3 cut(s) 355, 868, 936
SsiI CCGC 2 cut(s) 402, 405
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 2 cut(s) 282, 427
TaaI ACNGT 5 cut(s) 75, 129, 425, 644, 696
TaiI ACGT 1 cut(s) 660
TaqI TCGA 3 cut(s) 155, 556, 884
TaqII GACCGA 1 cut(s) 410
TasI AATT 3 cut(s) 355, 868, 936
TatI WGTACW 1 cut(s) 189
TauI GCSGC 2 cut(s) 405, 408
TfiI GAWTC 7 cut(s) 90, 105, 152, 271, 457, 752, 864
Tru1I TTAA 3 cut(s) 117, 569, 671
Tru9I TTAA 3 cut(s) 117, 569, 671
TscAI CASTG 2 cut(s) 632, 657
TseFI GTSAC 1 cut(s) 498
TseI GCWGC 1 cut(s) 602
Tsp45I GTSAC 1 cut(s) 498
TspDTI ATGAA 5 cut(s) 377, 470, 473, 744, 888
TspRI CASTG 2 cut(s) 632, 657
Van91I CCANNNNNTGG 1 cut(s) 598
VpaK11BI GGWCC 2 cut(s) 393, 425
XhoI CTCGAG 1 cut(s) 883
XmiI GTMKAC 1 cut(s) 613
XmnI GAANNNNTTC 1 cut(s) 852
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.