AT4G32020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005989
Physical Location & Seq
Forward (+)
15487914 .. 15490048
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32020.1

Sequence Viewer

Length: 546 bp
ATGGGCGTCGCTGTTCTAAATCCCCAGGATTGCTTGAAAGATCCATTCTCTCACATGAAACAACATCGTAATCCTAGCGCATGTCCCAACAGGCAGAAGAAGACGGTCTCCAACAACCGTACACGCCGGAGTCCTCCTCGTAGTCAATCCTCCAGATCTTCTTCTCCTCCTGTAGCGCCGCCGCTTCCTCCTCCTCGTGCGGCTGTCTCTGCTTTCGTTCCAAAGGGAACCCTTAAGAAGAGTCTTAGCAATACCGTTGCCGTTAGCCAGGTGAGAATCCTTAAGCGCGGCGAAGAAATCCCCAAGGAGACTTCAGATCTGGTTGTGGAAATGTCAGATCTGGGATCTACTCGTCGGATTGGACCAGATCCGGGTTTGATTCCGGGTCAGATCCGTTTATCTGGCCGCAAATCGAAATCAGCACCGTTTTACGCCGGTCCTGTGACCATGACCTCGCCGCCTCCTAGCGATGTCCCGCTTCCAGCTTTCTTCACGAAGAAGGCAACAAATGATCTCATCAGGATCCTTCGCCTAGACATCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNRC

Protein Analysis

181

Amino Acids

19.59

Weight (kDa)

10.97

Isoelectric Point (pI)

76.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 288
AciI CCGC 7 cut(s) 179, 182, 200, 288, 406, 458, 476
AclWI GGATC 6 cut(s) 35, 352, 362, 385, 517, 530
AcoI YGGCCR 1 cut(s) 403
AcuI CTGAAG 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 1 cut(s) 121
AfiI CCNNNNNNNGG 1 cut(s) 371
AflII CTTAAG 2 cut(s) 233, 281
AgsI TTSAA 1 cut(s) 37
AjnI CCWGG 2 cut(s) 24, 267
AluBI AGCT 1 cut(s) 485
AluI AGCT 1 cut(s) 485
Alw26I GTCTC 3 cut(s) 112, 211, 302
AlwI GGATC 6 cut(s) 35, 352, 362, 385, 517, 530
AoxI GGCC 1 cut(s) 403
AspLEI GCGC 3 cut(s) 80, 178, 288
AspS9I GGNCC 2 cut(s) 362, 437
AsuC2I CCSGG 2 cut(s) 372, 384
AsuHPI GGTGA 1 cut(s) 283
AvaII GGWCC 2 cut(s) 362, 437
BamHI GGATCC 1 cut(s) 522
BauI CACGAG 1 cut(s) 195
BbsI GAAGAC 1 cut(s) 107
BceAI ACGGC 1 cut(s) 245
BciT130I CCWGG 2 cut(s) 26, 269
BcnI CCSGG 2 cut(s) 372, 384
BcoDI GTCTC 3 cut(s) 112, 211, 302
BfaI CTAG 3 cut(s) 75, 465, 533
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 179
BfrI CTTAAG 2 cut(s) 233, 281
BglII AGATCT 3 cut(s) 155, 316, 337
BisI GCNGC 6 cut(s) 179, 182, 201, 289, 406, 458
BlsI GCNGC 6 cut(s) 180, 183, 202, 290, 407, 459
Bme1390I CCNGG 4 cut(s) 26, 269, 372, 384
Bme18I GGWCC 2 cut(s) 362, 437
BmgT120I GGNCC 2 cut(s) 362, 437
BmiI GGNNCC 2 cut(s) 229, 524
BmrFI CCNGG 4 cut(s) 26, 269, 372, 384
BpiI GAAGAC 1 cut(s) 107
BplI GAGNNNNNCTC 2 cut(s) 121, 153
BpmI CTGGAG 1 cut(s) 136
BpuMI CCSGG 2 cut(s) 372, 384
BsaHI GRCGYC 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 24, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse118I RCCGGY 1 cut(s) 434
BseBI CCWGG 2 cut(s) 26, 269
BseDI CCNNGG 2 cut(s) 24, 303
BseLI CCNNNNNNNGG 1 cut(s) 371
BseRI GAGGAG 4 cut(s) 126, 156, 180, 183
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 1 cut(s) 405
BsiSI CCGG 4 cut(s) 127, 371, 383, 435
BslFI GGGAC 2 cut(s) 69, 458
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 3 cut(s) 112, 211, 302
BsmFI GGGAC 2 cut(s) 69, 458
BsnI GGCC 1 cut(s) 405
Bso31I GGTCTC 1 cut(s) 112
Bsp143I GATC 9 cut(s) 40, 155, 316, 337, 344, 367, 390, 511, 522
BspACI CCGC 7 cut(s) 179, 182, 200, 288, 406, 458, 476
BspANI GGCC 1 cut(s) 405
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 229, 524
BspPI GGATC 6 cut(s) 35, 352, 362, 385, 517, 530
BspTI CTTAAG 2 cut(s) 233, 281
BspTNI GGTCTC 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 434
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 2 cut(s) 24, 303
BssMI GATC 9 cut(s) 40, 155, 316, 337, 344, 367, 390, 511, 522
BssNI GRCGYC 1 cut(s) 6
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 195
Bst2UI CCWGG 2 cut(s) 26, 269
Bst4CI ACNGT 4 cut(s) 106, 119, 256, 426
Bst6I CTCTTC 1 cut(s) 233
BstACI GRCGYC 1 cut(s) 6
BstAFI CTTAAG 2 cut(s) 233, 281
BstDEI CTNAG 1 cut(s) 245
BstFNI CGCG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 3 cut(s) 80, 178, 288
BstKTI GATC 9 cut(s) 43, 158, 319, 340, 347, 370, 393, 514, 525
BstMAI GTCTC 3 cut(s) 112, 211, 302
BstMBI GATC 9 cut(s) 40, 155, 316, 337, 344, 367, 390, 511, 522
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 2 cut(s) 26, 269
BstNSI RCATGY 1 cut(s) 84
BstSCI CCNGG 4 cut(s) 24, 267, 370, 382
BstSFI CTRYAG 1 cut(s) 171
BstUI CGCG 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 107
BstX2I RGATCY 8 cut(s) 40, 155, 316, 337, 344, 367, 390, 522
BstYI RGATCY 8 cut(s) 40, 155, 316, 337, 344, 367, 390, 522
BsuRI GGCC 1 cut(s) 405
BtgZI GCGATG 2 cut(s) 483, 523
CfoI GCGC 3 cut(s) 80, 178, 288
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 362, 437
Csp6I GTAC 1 cut(s) 120
CviAII CATG 3 cut(s) 55, 81, 448
CviJI RGCY 4 cut(s) 203, 267, 405, 485
CviKI_1 RGCY 4 cut(s) 203, 267, 405, 485
CviQI GTAC 1 cut(s) 120
DdeI CTNAG 1 cut(s) 245
DpnI GATC 9 cut(s) 42, 157, 318, 339, 346, 369, 392, 513, 524
DpnII GATC 9 cut(s) 40, 155, 316, 337, 344, 367, 390, 511, 522
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 112
Eco47I GGWCC 2 cut(s) 362, 437
Eco57I CTGAAG 1 cut(s) 297
EcoRII CCWGG 2 cut(s) 24, 267
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 3 cut(s) 58, 84, 451
FaiI YATR 3 cut(s) 56, 82, 449
FaqI GGGAC 2 cut(s) 69, 458
FatI CATG 3 cut(s) 54, 80, 447
FauI CCCGC 1 cut(s) 483
Fnu4HI GCNGC 6 cut(s) 179, 182, 201, 289, 406, 458
Fsp4HI GCNGC 6 cut(s) 179, 182, 201, 289, 406, 458
FspBI CTAG 3 cut(s) 75, 465, 533
GlaI GCGC 3 cut(s) 79, 177, 287
GluI GCNGC 6 cut(s) 179, 182, 201, 289, 406, 458
GsuI CTGGAG 1 cut(s) 136
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 1 cut(s) 405
HapII CCGG 4 cut(s) 127, 371, 383, 435
HhaI GCGC 3 cut(s) 80, 178, 288
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 3 cut(s) 58, 84, 451
Hin6I GCGC 3 cut(s) 78, 176, 286
HinP1I GCGC 3 cut(s) 78, 176, 286
HinfI GANTC 4 cut(s) 130, 241, 276, 379
HpaII CCGG 4 cut(s) 127, 371, 383, 435
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 122
Hpy188I TCNGA 4 cut(s) 316, 337, 357, 390
Hpy188III TCNNGA 3 cut(s) 153, 493, 520
Hpy8I GTNNAC 1 cut(s) 122
Hpy99I CGWCG 2 cut(s) 11, 357
HpyAV CCTTC 2 cut(s) 493, 536
HpyCH4III ACNGT 4 cut(s) 106, 119, 256, 426
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 1 cut(s) 245
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 3 cut(s) 58, 84, 451
HspAI GCGC 3 cut(s) 78, 176, 286
Kzo9I GATC 9 cut(s) 40, 155, 316, 337, 344, 367, 390, 511, 522
MaeI CTAG 3 cut(s) 75, 465, 533
MaeIII GTNAC 1 cut(s) 442
MalI GATC 9 cut(s) 42, 157, 318, 339, 346, 369, 392, 513, 524
MboI GATC 9 cut(s) 40, 155, 316, 337, 344, 367, 390, 511, 522
MboII GAAGA 8 cut(s) 109, 112, 150, 153, 250, 305, 481, 508
MflI RGATCY 8 cut(s) 40, 155, 316, 337, 344, 367, 390, 522
MlyI GAGTC 2 cut(s) 139, 250
MmeI TCCRAC 2 cut(s) 135, 335
MnlI CCTC 9 cut(s) 144, 147, 160, 177, 198, 201, 204, 463, 471
MseI TTAA 2 cut(s) 234, 282
MspCI CTTAAG 2 cut(s) 233, 281
MspI CCGG 4 cut(s) 127, 371, 383, 435
MspR9I CCNGG 4 cut(s) 26, 269, 372, 384
MvaI CCWGG 2 cut(s) 26, 269
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 209
NciI CCSGG 2 cut(s) 372, 384
NdeII GATC 9 cut(s) 40, 155, 316, 337, 344, 367, 390, 511, 522
NlaIII CATG 3 cut(s) 58, 84, 451
NlaIV GGNNCC 2 cut(s) 229, 524
NmuCI GTSAC 1 cut(s) 442
NspI RCATGY 1 cut(s) 84
PfeI GAWTC 2 cut(s) 276, 379
PkrI GCNGC 6 cut(s) 180, 183, 202, 290, 407, 459
PleI GAGTC 2 cut(s) 138, 249
PpsI GAGTC 2 cut(s) 138, 249
Psp6I CCWGG 2 cut(s) 24, 267
PspGI CCWGG 2 cut(s) 24, 267
PspN4I GGNNCC 2 cut(s) 229, 524
PspPI GGNCC 2 cut(s) 362, 437
PsuI RGATCY 8 cut(s) 40, 155, 316, 337, 344, 367, 390, 522
RsaI GTAC 1 cut(s) 121
RsaNI GTAC 1 cut(s) 120
SaqAI TTAA 2 cut(s) 234, 282
SatI GCNGC 6 cut(s) 179, 182, 201, 289, 406, 458
Sau3AI GATC 9 cut(s) 40, 155, 316, 337, 344, 367, 390, 511, 522
Sau96I GGNCC 2 cut(s) 362, 437
SchI GAGTC 2 cut(s) 139, 250
ScrFI CCNGG 4 cut(s) 26, 269, 372, 384
SetI ASST 3 cut(s) 273, 455, 487
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 2 cut(s) 362, 437
SmlI CTYRAG 2 cut(s) 233, 281
SmoI CTYRAG 2 cut(s) 233, 281
SsiI CCGC 7 cut(s) 179, 182, 200, 288, 406, 458, 476
SspMI CTAG 3 cut(s) 75, 465, 533
StyD4I CCNGG 4 cut(s) 24, 267, 370, 382
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 4 cut(s) 106, 119, 256, 426
TaqI TCGA 1 cut(s) 413
TauI GCSGC 6 cut(s) 181, 184, 203, 291, 408, 460
TfiI GAWTC 2 cut(s) 276, 379
Tru1I TTAA 2 cut(s) 234, 282
Tru9I TTAA 2 cut(s) 234, 282
TseFI GTSAC 1 cut(s) 442
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 1 cut(s) 71
TspGWI ACGGA 1 cut(s) 383
Vha464I CTTAAG 2 cut(s) 233, 281
VpaK11BI GGWCC 2 cut(s) 362, 437
XceI RCATGY 1 cut(s) 84
XspI CTAG 3 cut(s) 75, 465, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.