Rorug06G0269500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005989
Physical Location & Seq
Forward (+)
41659721 .. 41661902
2182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0269500.1

Sequence Viewer

Length: 999 bp
ATGGCAGCCATGGCGGCCAAACTTATGCATGCGGTTCAGTACGACGCTTACGGTGGAGGACCTTCTGGTTTAAAGCATGTTGAGGTTCCAATCCCCACTCCCAAAAAAGATGAGGTTTTGCTGAAATTGGAAGCGGCTAGCATAAACCCAGTTGATTGGAAAATTCAGAAAGGCGTAGCGCGCCCTATTTTGCCACGCAAATTTCCTTATACACCTGTTACTGATGTGGCCGGAGAGGTTTTGGAGGTTGGACAAGGAGTTCGGAAGTTTAAAGCAGGCGACAAAGTTGTGGCATATCTCCACATCGTTAATGGAGGTGGATTTGCTGAGTTTGCAACTGCTAGTGAGAGTTTGACGGTTACTAGGCCGCCTGAAGTTTCAGCAGCCGAAGCTGCAGGATTACCTATTGCTGGTCTCACAGCTCACCAGTGTCTCACCCAAGCTGCAGGAGTCAAGCTTGACGGCACTGGCCCACAGAAGAACATATTGGTTACTGCTGCCTCCGGTGGTGTGGGTCAGTATGCAGTCCAACTTGCAAAGCTGGGAAACTCCCACGTCACAGCTACATGTGGAGCTCGTAACATTGATTTCGTCAAGAGCTTAGGGGCCGATGAGGTTCTTGACTACAAGACCCCAGAAGGGGCAGCTCTGAAGAGCCCATCTGGTCGCAAATATGATGCTGTAATCCACTGTGCGACGGGCATTCCTTGGTGCACTTTTGAGCCGAATTTGAGTCCAAATGGGAAGGTTATAGACATTACTCCTGGTCCAAGTGCTTTTTTTACTTTCGCTCTAAAGAATCTCACCTTCTCCAAGAAGAAGCTAGTGCCGCTGCTCATGAATGCCAAGGCGGAGAATCTGGATTATCTTGTGAAGTTGGTGAAGGAAGGAAAGCTGAAGACAGTGATCGACTCAAAGTATCCTCTGAGCAAGGCTGAAGATGCTTGGGCTAAGAGTATTGATGGCCATGCTACTGGGAAGATCATTGTGGAGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNRC

Protein Analysis

332

Amino Acids

34.99

Weight (kDa)

9.03

Isoelectric Point (pI)

25.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 36 - 121 5.9e-15 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 170 - 282 1.4e-09 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 201 - 330 2.5e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 181
AciI CCGC 6 cut(s) 14, 32, 134, 368, 830, 851
AcoI YGGCCR 3 cut(s) 15, 228, 964
AcsI RAATTY 3 cut(s) 162, 200, 727
AcuI CTGAAG 4 cut(s) 393, 671, 917, 957
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 3 cut(s) 410, 639, 640
AflIII ACRYGT 1 cut(s) 566
AjiI CACGTC 1 cut(s) 556
AjnI CCWGG 1 cut(s) 763
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
Alw21I GWGCWC 2 cut(s) 577, 716
Alw26I GTCTC 2 cut(s) 419, 437
Alw44I GTGCAC 1 cut(s) 712
AoxI GGCC 6 cut(s) 15, 228, 365, 469, 606, 964
ApaLI GTGCAC 1 cut(s) 712
ApeKI GCWGC 7 cut(s) 5, 383, 392, 443, 497, 644, 832
ApoI RAATTY 3 cut(s) 162, 200, 727
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AspLEI GCGC 2 cut(s) 181, 183
AspS9I GGNCC 4 cut(s) 59, 470, 606, 767
AsuHPI GGTGA 4 cut(s) 416, 427, 796, 892
AsuNHI GCTAGC 1 cut(s) 137
AvaII GGWCC 2 cut(s) 59, 767
BaeGI GKGCMC 1 cut(s) 716
BalI TGGCCA 1 cut(s) 966
BanII GRGCYC 2 cut(s) 577, 659
BbsI GAAGAC 1 cut(s) 905
Bbv12I GWGCWC 2 cut(s) 577, 716
BbvI GCAGC 7 cut(s) 17, 379, 395, 430, 484, 656, 819
BccI CCATC 2 cut(s) 667, 956
BceAI ACGGC 1 cut(s) 478
BciT130I CCWGG 1 cut(s) 765
BciVI GTATCC 1 cut(s) 930
BcoDI GTCTC 2 cut(s) 419, 437
BfaI CTAG 4 cut(s) 138, 342, 363, 824
BfmI CTRYAG 2 cut(s) 393, 444
BfuI GTATCC 1 cut(s) 930
BglI GCCNNNNNGGC 1 cut(s) 14
Bme1390I CCNGG 1 cut(s) 765
Bme18I GGWCC 2 cut(s) 59, 767
BmgBI CACGTC 1 cut(s) 556
BmgT120I GGNCC 4 cut(s) 59, 470, 606, 767
BmiI GGNNCC 3 cut(s) 87, 607, 993
BmrFI CCNGG 1 cut(s) 765
BmrI ACTGGG 2 cut(s) 143, 984
BmsI GCATC 2 cut(s) 667, 931
BmtI GCTAGC 1 cut(s) 141
BmuI ACTGGG 2 cut(s) 143, 984
BpiI GAAGAC 1 cut(s) 905
Bpu10I CCTNAGC 1 cut(s) 601
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 3 cut(s) 9, 707, 846
BsaWI WCCGGW 1 cut(s) 503
Bsc4I CCNNNNNNNGG 3 cut(s) 410, 639, 640
Bse1I ACTGG 4 cut(s) 149, 427, 472, 979
BseBI CCWGG 1 cut(s) 765
BseDI CCNNGG 3 cut(s) 9, 707, 846
BseLI CCNNNNNNNGG 3 cut(s) 410, 639, 640
BseMII CTCAG 2 cut(s) 318, 917
BseNI ACTGG 4 cut(s) 149, 427, 472, 979
BsePI GCGCGC 1 cut(s) 179
BseSI GKGCMC 1 cut(s) 716
BseXI GCAGC 7 cut(s) 17, 379, 395, 430, 484, 656, 819
BseYI CCCAGC 1 cut(s) 541
Bsh1236I CGCG 1 cut(s) 181
BshFI GGCC 6 cut(s) 17, 230, 367, 471, 608, 966
BsiHKAI GWGCWC 2 cut(s) 577, 716
BsiSI CCGG 2 cut(s) 231, 504
BslI CCNNNNNNNGG 3 cut(s) 410, 639, 640
BsmAI GTCTC 2 cut(s) 419, 437
BsmI GAATGC 2 cut(s) 702, 847
BsnI GGCC 6 cut(s) 17, 230, 367, 471, 608, 966
Bso31I GGTCTC 1 cut(s) 419
Bsp1286I GDGCHC 3 cut(s) 577, 659, 716
Bsp143I GATC 2 cut(s) 906, 981
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 6 cut(s) 14, 32, 134, 368, 830, 851
BspANI GGCC 6 cut(s) 17, 230, 367, 471, 608, 966
BspCNI CTCAG 2 cut(s) 319, 918
BspFNI CGCG 1 cut(s) 181
BspHI TCATGA 1 cut(s) 837
BspLI GGNNCC 3 cut(s) 87, 607, 993
BspMAI CTGCAG 2 cut(s) 397, 448
BspOI GCTAGC 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 647
BspTNI GGTCTC 1 cut(s) 419
BsrI ACTGG 4 cut(s) 149, 427, 472, 979
BssECI CCNNGG 3 cut(s) 9, 707, 846
BssHII GCGCGC 1 cut(s) 179
BssMI GATC 2 cut(s) 906, 981
BssT1I CCWWGG 3 cut(s) 9, 707, 846
Bst2UI CCWGG 1 cut(s) 765
Bst4CI ACNGT 4 cut(s) 53, 358, 692, 904
Bst6I CTCTTC 1 cut(s) 647
BstC8I GCNNGC 4 cut(s) 30, 139, 181, 277
BstDEI CTNAG 4 cut(s) 327, 601, 926, 951
BstDSI CCRYGG 1 cut(s) 9
BstFNI CGCG 1 cut(s) 181
BstHHI GCGC 2 cut(s) 181, 183
BstKTI GATC 2 cut(s) 909, 984
BstMAI GTCTC 2 cut(s) 419, 437
BstMBI GATC 2 cut(s) 906, 981
BstMWI GCNNNNNNNGC 8 cut(s) 11, 14, 180, 332, 389, 392, 829, 941
BstNI CCWGG 1 cut(s) 765
BstNSI RCATGY 3 cut(s) 32, 80, 570
BstSCI CCNGG 1 cut(s) 763
BstSFI CTRYAG 2 cut(s) 393, 444
BstSLI GKGCMC 1 cut(s) 716
BstUI CGCG 1 cut(s) 181
BstV1I GCAGC 7 cut(s) 17, 379, 395, 430, 484, 656, 819
BstV2I GAAGAC 1 cut(s) 905
BstXI CCANNNNNNTGG 2 cut(s) 156, 974
BsuI GTATCC 1 cut(s) 930
BsuRI GGCC 6 cut(s) 17, 230, 367, 471, 608, 966
BtgI CCRYGG 1 cut(s) 9
BtrI CACGTC 1 cut(s) 556
BtsIMutI CAGTG 4 cut(s) 434, 465, 688, 909
Cac8I GCNNGC 4 cut(s) 30, 139, 181, 277
CciI TCATGA 1 cut(s) 837
CfoI GCGC 2 cut(s) 181, 183
Cfr13I GGNCC 4 cut(s) 59, 470, 606, 767
CseI GACGC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 40
CviAII CATG 6 cut(s) 10, 29, 77, 567, 838, 968
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 4 cut(s) 327, 601, 926, 951
DpnI GATC 2 cut(s) 908, 983
DpnII GATC 2 cut(s) 906, 981
DraI TTTAAA 2 cut(s) 72, 271
EaeI YGGCCR 3 cut(s) 15, 228, 964
Eam1104I CTCTTC 1 cut(s) 647
EarI CTCTTC 1 cut(s) 647
EciI GGCGGA 1 cut(s) 866
Ecl136II GAGCTC 1 cut(s) 575
Eco130I CCWWGG 3 cut(s) 9, 707, 846
Eco24I GRGCYC 2 cut(s) 577, 659
Eco31I GGTCTC 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 59, 767
Eco53kI GAGCTC 1 cut(s) 575
Eco57I CTGAAG 4 cut(s) 393, 671, 917, 957
EcoICRI GAGCTC 1 cut(s) 575
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 763
EcoT14I CCWWGG 3 cut(s) 9, 707, 846
EcoT22I ATGCAT 1 cut(s) 30
EcoT38I GRGCYC 2 cut(s) 577, 659
ErhI CCWWGG 3 cut(s) 9, 707, 846
FaeI CATG 6 cut(s) 13, 32, 80, 570, 841, 971
FatI CATG 6 cut(s) 9, 28, 76, 566, 837, 967
FriOI GRGCYC 2 cut(s) 577, 659
FspBI CTAG 4 cut(s) 138, 342, 363, 824
GlaI GCGC 2 cut(s) 180, 182
GsaI CCCAGC 1 cut(s) 545
HaeIII GGCC 6 cut(s) 17, 230, 367, 471, 608, 966
HapII CCGG 2 cut(s) 231, 504
HgaI GACGC 1 cut(s) 53
HhaI GCGC 2 cut(s) 181, 183
Hin1II CATG 6 cut(s) 13, 32, 80, 570, 841, 971
Hin6I GCGC 2 cut(s) 179, 181
HinP1I GCGC 2 cut(s) 179, 181
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 5 cut(s) 450, 733, 799, 856, 911
HpaII CCGG 2 cut(s) 231, 504
HphI GGTGA 4 cut(s) 416, 427, 796, 892
Hpy166II GTNNAC 1 cut(s) 714
Hpy188I TCNGA 4 cut(s) 168, 264, 651, 927
Hpy188III TCNNGA 4 cut(s) 595, 620, 838, 860
Hpy8I GTNNAC 1 cut(s) 714
Hpy99I CGWCG 2 cut(s) 47, 700
HpyAV CCTTC 6 cut(s) 72, 632, 739, 817, 877, 881
HpyCH4III ACNGT 4 cut(s) 53, 358, 692, 904
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 7 cut(s) 28, 335, 395, 446, 524, 536, 714
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 14, 180, 332, 389, 392, 829, 941
HpyF3I CTNAG 4 cut(s) 327, 601, 926, 951
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 6 cut(s) 13, 32, 80, 570, 841, 971
HspAI GCGC 2 cut(s) 179, 181
Kzo9I GATC 2 cut(s) 906, 981
LguI GCTCTTC 1 cut(s) 647
LmnI GCTCC 2 cut(s) 572, 991
Lsp1109I GCAGC 7 cut(s) 17, 379, 395, 430, 484, 656, 819
LweI GCATC 2 cut(s) 667, 931
MaeI CTAG 4 cut(s) 138, 342, 363, 824
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 5 cut(s) 217, 358, 490, 556, 578
MalI GATC 2 cut(s) 908, 983
MboI GATC 2 cut(s) 906, 981
MboII GAAGA 6 cut(s) 490, 664, 829, 910, 950, 991
MhlI GDGCHC 3 cut(s) 577, 659, 716
MlsI TGGCCA 1 cut(s) 966
MluCI AATT 4 cut(s) 125, 162, 200, 727
MluNI TGGCCA 1 cut(s) 966
MlyI GAGTC 3 cut(s) 459, 742, 905
MmeI TCCRAC 2 cut(s) 229, 553
MnlI CCTC 9 cut(s) 50, 76, 106, 229, 238, 308, 511, 607, 933
Mox20I TGGCCA 1 cut(s) 966
Mph1103I ATGCAT 1 cut(s) 30
MscI TGGCCA 1 cut(s) 966
MseI TTAA 4 cut(s) 71, 270, 309, 997
Msp20I TGGCCA 1 cut(s) 966
MspA1I CMGCKG 1 cut(s) 832
MspI CCGG 2 cut(s) 231, 504
MspR9I CCNGG 1 cut(s) 765
Mva1269I GAATGC 2 cut(s) 702, 847
MvaI CCWGG 1 cut(s) 765
MvnI CGCG 1 cut(s) 181
MwoI GCNNNNNNNGC 8 cut(s) 11, 14, 180, 332, 389, 392, 829, 941
NcoI CCATGG 1 cut(s) 9
NdeII GATC 2 cut(s) 906, 981
NheI GCTAGC 1 cut(s) 137
NlaIII CATG 6 cut(s) 13, 32, 80, 570, 841, 971
NlaIV GGNNCC 3 cut(s) 87, 607, 993
NmuCI GTSAC 1 cut(s) 556
NsiI ATGCAT 1 cut(s) 30
NspI RCATGY 3 cut(s) 32, 80, 570
PaeI GCATGC 1 cut(s) 32
PagI TCATGA 1 cut(s) 837
PauI GCGCGC 1 cut(s) 179
PciI ACATGT 1 cut(s) 566
PciSI GCTCTTC 1 cut(s) 647
PctI GAATGC 2 cut(s) 702, 847
PfeI GAWTC 2 cut(s) 799, 856
PleI GAGTC 3 cut(s) 458, 741, 905
PpsI GAGTC 3 cut(s) 458, 741, 905
PpuMI RGGWCCY 1 cut(s) 59
PscI ACATGT 1 cut(s) 566
Psp124BI GAGCTC 1 cut(s) 577
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 763
PspFI CCCAGC 1 cut(s) 541
PspGI CCWGG 1 cut(s) 763
PspN4I GGNNCC 3 cut(s) 87, 607, 993
PspPI GGNCC 4 cut(s) 59, 470, 606, 767
PspPPI RGGWCCY 1 cut(s) 59
PstI CTGCAG 2 cut(s) 397, 448
PteI GCGCGC 1 cut(s) 179
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SacI GAGCTC 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 647
SaqAI TTAA 4 cut(s) 71, 270, 309, 997
Sau3AI GATC 2 cut(s) 906, 981
Sau96I GGNCC 4 cut(s) 59, 470, 606, 767
SchI GAGTC 3 cut(s) 459, 742, 905
ScrFI CCNGG 1 cut(s) 765
SduI GDGCHC 3 cut(s) 577, 659, 716
SfaNI GCATC 2 cut(s) 667, 931
SfcI CTRYAG 2 cut(s) 393, 444
SinI GGWCC 2 cut(s) 59, 767
SphI GCATGC 1 cut(s) 32
Sse9I AATT 4 cut(s) 125, 162, 200, 727
SsiI CCGC 6 cut(s) 14, 32, 134, 368, 830, 851
SspMI CTAG 4 cut(s) 138, 342, 363, 824
SstI GAGCTC 1 cut(s) 577
StyD4I CCNGG 1 cut(s) 763
StyI CCWWGG 3 cut(s) 9, 707, 846
TaaI ACNGT 4 cut(s) 53, 358, 692, 904
TaiI ACGT 1 cut(s) 558
TaqI TCGA 1 cut(s) 909
TasI AATT 4 cut(s) 125, 162, 200, 727
TauI GCSGC 4 cut(s) 17, 137, 370, 832
TfiI GAWTC 2 cut(s) 799, 856
Tru1I TTAA 4 cut(s) 71, 270, 309, 997
Tru9I TTAA 4 cut(s) 71, 270, 309, 997
TscAI CASTG 4 cut(s) 434, 472, 695, 909
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 7 cut(s) 5, 383, 392, 443, 497, 644, 832
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 1 cut(s) 854
TspRI CASTG 4 cut(s) 434, 472, 695, 909
VneI GTGCAC 1 cut(s) 712
VpaK11BI GGWCC 2 cut(s) 59, 767
XapI RAATTY 3 cut(s) 162, 200, 727
XceI RCATGY 3 cut(s) 32, 80, 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XspI CTAG 4 cut(s) 138, 342, 363, 824
Zsp2I ATGCAT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.