AT4G33590

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004099
Physical Location & Seq
Reverse (-)
16142497 .. 16144024
1528 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33590.1

Sequence Viewer

Length: 1401 bp
ATGACAACTAAAGCTAGTGTTTGGCGAAGGCTAGTTACTTGCTTATCCTCTCTTAAGTTCTACTTGAACGTCTTGTGCCTCGTTGTCACTGTTTTTGTTCTTCTCCAAATCTGTTCATTTCAAATTACTCAACGTTCTCTTTCACTCCCTCCTGCACTCTTGACGTATCTGAAACACAATCATGAAGAAGTATCAGAGAACAAGACGGCTTCCTTGGTGGAGAAGCTACGTGAATCCGTGACATTCCTTCCACTCAAAGACTATCGTTTTTCGAACAAACCACTTGAAGGTCATACTTGGTTCATGAGCTCTCTCTATGATAATCAAACCAAAGGTGAGGCTCAGTATCAAGAATTTCCTTCAGATTCTTCGAAAGGAAGGCTTTTGTGTTTGAAAGGGGTTGATGAACATGATGGGTCATGGAACTCCTACGCGCTGGCTTGGCCTGAAGCTCTTCCTACCAATGCCATTCTTCAGGATGGCTTGACGTTTGTCTCTTACAATCAATATGATTATGGTAACTTGTGGCATGGACTAACGGCAGTAGTTCCATTTATTGCTTGGAGCTTAAGAAATCAATGTGAAAAGCCTCAAAAATGGGTTTTATACCACTGGGGAGAACTAAGGTTCGGAATGGGGCATTGGCTGAGCGAGATAGTCACAGCCACTTACGGTCAAGAACCGGACTTTTTACGGTTTGTTGATGATGATAAGCCTGTTTGTTTCGAGAAGGCGGTTGTTATGAGACATAATGAAGGTGGAATGTCAAGGGAGAGAAGAATGGAAGCTTTTGATCTCATTAGATGCAAAGCGAGGAATTACTGTAATATCAGCTCATCTGTGGCATCAAAACCGCGAATCGGTATGACATTACTATTGAGAACCGGAGCCAGATCTTTCAGAAATGAATCAATGGTGATTGATGTCTTTAAGAAAGAGTGTAAGAGAGTAGATGGGTGTGAAATTAGTGTTTCATATTCGAATAATCTATCATTTTGTGAGCAAGTGGAGCTAATGAAGAAGACCGATGTGTTAGTGTCACCGCACGGTGCACAACTCACTAACTTGTTTCTAATGGATAAAAATAGTAGTGTAATGGAGTTTTTCCCCAAAGGATGGCTTAAGCTTGCAGGAGTTGGCCAGCTTGTGTTCCAATGGGGAGCTAATTGGTCAGGGATGAGACACGAAGGCTCTTGGCACGACCCGGTTGGCGAAATATGTCAATTCCCCGATACAGACAGGCGGTGTATGTCCATCTACAAGAACGCTATGATTGGATACAATGAGACTTATTTTGGCGAGTGGGCAAGAAGAGTTTTGGGTAAGTTTAGCATAAGAGAGATGAAAGAGCTTGCAGAGTGCAACCACAGTAATAGTTCAATATTAGATGTATGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF563

Protein Analysis

466

Amino Acids

53.54

Weight (kDa)

7.85

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 212 - 366 3.5e-18 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1116
AccII CGCG 2 cut(s) 434, 856
AciI CCGC 4 cut(s) 734, 854, 1043, 1243
AclI AACGTT 1 cut(s) 133
AcoI YGGCCR 1 cut(s) 1138
AcsI RAATTY 1 cut(s) 353
AcuI CTGAAG 3 cut(s) 345, 458, 468
AfiI CCNNNNNNNGG 3 cut(s) 287, 860, 1116
AflII CTTAAG 3 cut(s) 53, 568, 1121
AgsI TTSAA 5 cut(s) 67, 122, 287, 394, 1380
AjuI GAANNNNNNNTTGG 2 cut(s) 625, 657
Alw21I GWGCWC 2 cut(s) 311, 1054
Alw26I GTCTC 4 cut(s) 499, 739, 1174, 1280
Alw44I GTGCAC 1 cut(s) 1050
AoxI GGCC 2 cut(s) 443, 1138
ApaLI GTGCAC 1 cut(s) 1050
ApoI RAATTY 1 cut(s) 353
Asp700I GAANNNNTTC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 436
AsuC2I CCSGG 1 cut(s) 1205
AsuHPI GGTGA 3 cut(s) 347, 928, 1032
AsuII TTCGAA 3 cut(s) 272, 371, 980
BaeGI GKGCMC 1 cut(s) 1054
BalI TGGCCA 1 cut(s) 1140
BanII GRGCYC 1 cut(s) 311
BarI GAAGNNNNNNTAC 2 cut(s) 19, 51
BbsI GAAGAC 1 cut(s) 1028
Bbv12I GWGCWC 2 cut(s) 311, 1054
BccI CCATC 5 cut(s) 407, 473, 947, 1110, 1262
BceAI ACGGC 2 cut(s) 222, 555
BciVI GTATCC 1 cut(s) 1271
BcnI CCSGG 1 cut(s) 1205
BcoDI GTCTC 4 cut(s) 499, 739, 1174, 1280
BfaI CTAG 2 cut(s) 15, 32
BfrI CTTAAG 3 cut(s) 53, 568, 1121
BfuI GTATCC 1 cut(s) 1271
BglII AGATCT 1 cut(s) 893
BlpI GCTNAGC 1 cut(s) 647
Bme1390I CCNGG 1 cut(s) 1205
BmiI GGNNCC 1 cut(s) 889
BmrFI CCNGG 1 cut(s) 1205
BmrI ACTGGG 1 cut(s) 622
BmsI GCATC 2 cut(s) 794, 854
BmuI ACTGGG 1 cut(s) 622
BoxI GACNNNNGTC 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 1028
Bpu1102I GCTNAGC 1 cut(s) 647
Bpu14I TTCGAA 3 cut(s) 272, 371, 980
BpuMI CCSGG 1 cut(s) 1205
BsaAI YACGTR 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 2 cut(s) 682, 884
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 860, 1116
Bse1I ACTGG 1 cut(s) 617
BseDI CCNNGG 1 cut(s) 213
BseGI GGATG 3 cut(s) 484, 1121, 1182
BseLI CCNNNNNNNGG 3 cut(s) 287, 860, 1116
BseMII CTCAG 2 cut(s) 356, 638
BseNI ACTGG 1 cut(s) 617
BseSI GKGCMC 1 cut(s) 1054
BsgI GTGCAG 1 cut(s) 138
Bsh1236I CGCG 2 cut(s) 434, 856
BshFI GGCC 2 cut(s) 445, 1140
BsiHKAI GWGCWC 2 cut(s) 311, 1054
BsiSI CCGG 3 cut(s) 683, 885, 1205
BslI CCNNNNNNNGG 3 cut(s) 287, 860, 1116
BsmAI GTCTC 4 cut(s) 499, 739, 1174, 1280
BsnI GGCC 2 cut(s) 445, 1140
Bsp119I TTCGAA 3 cut(s) 272, 371, 980
Bsp1286I GDGCHC 2 cut(s) 311, 1054
Bsp143I GATC 2 cut(s) 793, 893
Bsp1720I GCTNAGC 1 cut(s) 647
BspACI CCGC 4 cut(s) 734, 854, 1043, 1243
BspANI GGCC 2 cut(s) 445, 1140
BspCNI CTCAG 2 cut(s) 355, 639
BspFNI CGCG 2 cut(s) 434, 856
BspHI TCATGA 2 cut(s) 181, 303
BspLI GGNNCC 1 cut(s) 889
BspQI GCTCTTC 1 cut(s) 459
BspT104I TTCGAA 3 cut(s) 272, 371, 980
BspTI CTTAAG 3 cut(s) 53, 568, 1121
BsrI ACTGG 1 cut(s) 617
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 2 cut(s) 793, 893
BssT1I CCWWGG 1 cut(s) 213
Bst4CI ACNGT 6 cut(s) 91, 674, 696, 824, 1049, 1370
Bst6I CTCTTC 2 cut(s) 459, 1306
BstAFI CTTAAG 3 cut(s) 53, 568, 1121
BstBAI YACGTR 1 cut(s) 230
BstBI TTCGAA 3 cut(s) 272, 371, 980
BstC8I GCNNGC 4 cut(s) 438, 1128, 1142, 1353
BstDEI CTNAG 3 cut(s) 342, 623, 647
BstF5I GGATG 3 cut(s) 484, 1121, 1182
BstFNI CGCG 2 cut(s) 434, 856
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 2 cut(s) 796, 896
BstMAI GTCTC 4 cut(s) 499, 739, 1174, 1280
BstMBI GATC 2 cut(s) 793, 893
BstMWI GCNNNNNNNGC 2 cut(s) 442, 1009
BstPAI GACNNNNGTC 1 cut(s) 491
BstSCI CCNGG 1 cut(s) 1203
BstSLI GKGCMC 1 cut(s) 1054
BstUI CGCG 2 cut(s) 434, 856
BstV2I GAAGAC 1 cut(s) 1028
BstX2I RGATCY 1 cut(s) 893
BstYI RGATCY 1 cut(s) 893
BsuI GTATCC 1 cut(s) 1271
BsuRI GGCC 2 cut(s) 445, 1140
BtsCI GGATG 3 cut(s) 484, 1121, 1182
BtsIMutI CAGTG 2 cut(s) 87, 610
Cac8I GCNNGC 4 cut(s) 438, 1128, 1142, 1353
CciI TCATGA 2 cut(s) 181, 303
CfoI GCGC 1 cut(s) 436
CviAII CATG 5 cut(s) 182, 304, 410, 420, 530
DdeI CTNAG 3 cut(s) 342, 623, 647
DpnI GATC 2 cut(s) 795, 895
DpnII GATC 2 cut(s) 793, 893
EaeI YGGCCR 1 cut(s) 1138
Eam1104I CTCTTC 2 cut(s) 459, 1306
EarI CTCTTC 2 cut(s) 459, 1306
Ecl136II GAGCTC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 311
Eco53kI GAGCTC 1 cut(s) 309
Eco57I CTGAAG 3 cut(s) 345, 458, 468
EcoICRI GAGCTC 1 cut(s) 309
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 5 cut(s) 185, 307, 413, 423, 533
FalI AAGNNNNNCTT 6 cut(s) 47, 79, 366, 398, 1104, 1136
FatI CATG 5 cut(s) 181, 303, 409, 419, 529
FokI GGATG 3 cut(s) 491, 1128, 1189
FriOI GRGCYC 1 cut(s) 311
FspBI CTAG 2 cut(s) 15, 32
GlaI GCGC 1 cut(s) 435
HaeIII GGCC 2 cut(s) 445, 1140
HapII CCGG 3 cut(s) 683, 885, 1205
HhaI GCGC 1 cut(s) 436
Hin1II CATG 5 cut(s) 185, 307, 413, 423, 533
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HindIII AAGCTT 2 cut(s) 786, 1124
HinfI GANTC 4 cut(s) 233, 365, 858, 908
HpaII CCGG 3 cut(s) 683, 885, 1205
HphI GGTGA 3 cut(s) 347, 928, 1032
Hpy166II GTNNAC 1 cut(s) 1052
Hpy188I TCNGA 5 cut(s) 171, 196, 364, 632, 902
Hpy188III TCNNGA 7 cut(s) 160, 182, 304, 350, 476, 677, 727
Hpy8I GTNNAC 1 cut(s) 1052
HpyAV CCTTC 8 cut(s) 21, 257, 281, 369, 372, 724, 749, 1181
HpyCH4III ACNGT 6 cut(s) 91, 674, 696, 824, 1049, 1370
HpyCH4IV ACGT 5 cut(s) 69, 133, 164, 229, 488
HpyCH4V TGCA 6 cut(s) 155, 807, 1052, 1130, 1355, 1362
HpyF10VI GCNNNNNNNGC 2 cut(s) 442, 1009
HpyF3I CTNAG 3 cut(s) 342, 623, 647
HpySE526I ACGT 5 cut(s) 69, 133, 164, 229, 488
Hsp92II CATG 5 cut(s) 185, 307, 413, 423, 533
HspAI GCGC 1 cut(s) 434
Kzo9I GATC 2 cut(s) 793, 893
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 4 cut(s) 564, 887, 1009, 1160
LweI GCATC 2 cut(s) 794, 854
MaeI CTAG 2 cut(s) 15, 32
MaeII ACGT 5 cut(s) 69, 133, 164, 229, 488
MaeIII GTNAC 6 cut(s) 34, 85, 238, 518, 658, 1038
MalI GATC 2 cut(s) 795, 895
MboI GATC 2 cut(s) 793, 893
MboII GAAGA 9 cut(s) 92, 197, 360, 446, 464, 789, 1030, 1033, 1323
MflI RGATCY 1 cut(s) 893
MhlI GDGCHC 2 cut(s) 311, 1054
MlsI TGGCCA 1 cut(s) 1140
MluCI AATT 6 cut(s) 123, 353, 817, 963, 1165, 1223
MluNI TGGCCA 1 cut(s) 1140
MnlI CCTC 6 cut(s) 58, 89, 159, 331, 600, 807
Mox20I TGGCCA 1 cut(s) 1140
MroXI GAANNNNTTC 1 cut(s) 453
MscI TGGCCA 1 cut(s) 1140
MseI TTAA 4 cut(s) 54, 569, 930, 1122
MslI CAYNNNNRTG 1 cut(s) 180
Msp20I TGGCCA 1 cut(s) 1140
MspCI CTTAAG 3 cut(s) 53, 568, 1121
MspI CCGG 3 cut(s) 683, 885, 1205
MspR9I CCNGG 1 cut(s) 1205
MvnI CGCG 2 cut(s) 434, 856
MwoI GCNNNNNNNGC 2 cut(s) 442, 1009
NciI CCSGG 1 cut(s) 1205
NdeII GATC 2 cut(s) 793, 893
NlaIII CATG 5 cut(s) 185, 307, 413, 423, 533
NlaIV GGNNCC 1 cut(s) 889
NmuCI GTSAC 4 cut(s) 85, 238, 658, 1038
NspV TTCGAA 3 cut(s) 272, 371, 980
PagI TCATGA 2 cut(s) 181, 303
PciSI GCTCTTC 1 cut(s) 459
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 4 cut(s) 233, 365, 858, 908
PflMI CCANNNNNTGG 1 cut(s) 1116
Ppu21I YACGTR 1 cut(s) 230
PshAI GACNNNNGTC 1 cut(s) 491
Psp124BI GAGCTC 1 cut(s) 311
Psp1406I AACGTT 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 889
PsuI RGATCY 1 cut(s) 893
RseI CAYNNNNRTG 1 cut(s) 180
SacI GAGCTC 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 4 cut(s) 54, 569, 930, 1122
Sau3AI GATC 2 cut(s) 793, 893
ScrFI CCNGG 1 cut(s) 1205
SduI GDGCHC 2 cut(s) 311, 1054
SfaNI GCATC 2 cut(s) 794, 854
SfuI TTCGAA 3 cut(s) 272, 371, 980
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 3 cut(s) 53, 568, 1121
SmoI CTYRAG 3 cut(s) 53, 568, 1121
Sse9I AATT 6 cut(s) 123, 353, 817, 963, 1165, 1223
SsiI CCGC 4 cut(s) 734, 854, 1043, 1243
SspI AATATT 1 cut(s) 1383
SspMI CTAG 2 cut(s) 15, 32
SstI GAGCTC 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 1203
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 6 cut(s) 91, 674, 696, 824, 1049, 1370
TaiI ACGT 5 cut(s) 72, 136, 167, 232, 491
TaqI TCGA 4 cut(s) 272, 371, 726, 980
TaqII GACCGA 1 cut(s) 1040
TasI AATT 6 cut(s) 123, 353, 817, 963, 1165, 1223
TfiI GAWTC 4 cut(s) 233, 365, 858, 908
Tru1I TTAA 4 cut(s) 54, 569, 930, 1122
Tru9I TTAA 4 cut(s) 54, 569, 930, 1122
TscAI CASTG 2 cut(s) 94, 617
TseFI GTSAC 4 cut(s) 85, 238, 658, 1038
Tsp45I GTSAC 4 cut(s) 85, 238, 658, 1038
TspDTI ATGAA 9 cut(s) 105, 198, 292, 420, 768, 921, 963, 1031, 1358
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 2 cut(s) 94, 617
Van91I CCANNNNNTGG 1 cut(s) 1116
Vha464I CTTAAG 3 cut(s) 53, 568, 1121
VneI GTGCAC 1 cut(s) 1050
XapI RAATTY 1 cut(s) 353
XcmI CCANNNNNNNNNTGG 1 cut(s) 558
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 2 cut(s) 15, 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.