MD15G1380300.v1.1

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004099
Physical Location & Seq
Forward (+)
46774687 .. 46776475
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1380300.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGGAAGTGGAAAGCAAGTCCATGCTGTTCACAAAAAAGAAGAGGTTTTCTCACTCAACCATTCTCAGCACCATTTCCCTCTTCTCACTATTTCTGATTCTTATCCTATTTCACGCACAACCCTCTCCTTCCTTCTCTCCTGCCTCAAAACCCGGAGACTCGAAACTCCGAAACTCGGTTACATTTCTTCCTCTCAAAGACACAAGGTTTGCCAACACTGCAATGGAGGGAAACACTTGGTTCATGAGCTCCTTGAGTGACACATTTGAAGAAAATGAATCCGAATACCTTCATTTCCCTTCGGATGCATCCAACGGAAGGCTACTGTGCATCAAAGGGAACGACATAAAAGATGGCACCAAAAACTCATATGCCTTGGCATATCCTGAAGGTTTACCAAACTCCACCACGTTCTTGAAAGGCCTTGCGTTTGTGTCCGACACATTCTATGACTACGGAAACTTGTGGCACGGGTTAACCGCCATGATGCCTTTCGTCGGTTGGTCTATGAAAAATGGATGCGCAAAGCCGGCAAGATTGGTACTTTTCCACTCGGGAGAGCTGAGAGTTACAGAGAAAATGGGGTTGTGGATTCAGAATTTGATGCAAGCGAATTTCGGGCAAGTGCAGGTCGAAGAGTTCGAGAAGGGAGAAGGGCCTTATTGTTTCGAGAAGGCGGTTGTGATGAGGCACAATGTTGGGAAAATGGGGAAGAAGAAGAAGCTTCAAGTGTCCGACTTGTTGAGATGTAAAGCAAGAGAGTTTTGTGGCATAAACCCTAATGGCAGACGCAGAGAGGTCAATGTGAGAGGGGAGCCAAGCATTAGGTTGACGTTGCTAATGAGGAGAGGATCAAGATCGTTCAAGAATCCGACGGCTGTGACTGCTGTGTTCTCAAGGGAGTGCGTGGCAGTGGATGGGTGCACGTTAAAGGTGGTTCAGTCTGAAGATCTGGATTTCTGTGATCAGGTTAGACTAATGACAAACACAGACGTCCTTGCATCCCCGCATGGAGCCCAGCTAACAAACATGCTCTTCATGGATAGAAACAGTAGCATAATGGAGTTCTTTCCCAAAGGATGGCTGGAGCTTGCAGGGATAGGTCAGTATGCTCATCACTGGATGGCCGATCAATCCGGTATGACGCACCGGGGTGCTTGGTGGGATCCCTATGCCGATAAAGAATGCCCGGAACCTAACAAACAACTCGAATGCTTTCTGTTTTACAAAGACGGCCGAGTTGGTCACAATGAAACCTACTTTGCAGAGTGGACACGAACTGTCCTCAAACAAGTAAGAACAAGCAAGTTAGAACAAGACATCGAGAGTTCGCAGAGAGATTCAAACGTTTGCCTATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF563

Protein Analysis

454

Amino Acids

51.4

Weight (kDa)

8.49

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 192 - 353 8.9e-15 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 996
Acc16I TGCGCA 1 cut(s) 523
Acc36I ACCTGC 1 cut(s) 619
AccB1I GGYRCC 1 cut(s) 356
AccB7I CCANNNNNTGG 1 cut(s) 1080
AciI CCGC 3 cut(s) 480, 677, 1007
AclI AACGTT 1 cut(s) 1347
AclWI GGATC 3 cut(s) 859, 1160, 1173
AcoI YGGCCR 2 cut(s) 1125, 1234
AcsI RAATTY 2 cut(s) 598, 613
AcuI CTGAAG 2 cut(s) 408, 966
AcyI GRCGYC 1 cut(s) 993
AfaI GTAC 1 cut(s) 543
AfiI CCNNNNNNNGG 4 cut(s) 175, 318, 497, 1080
AgsI TTSAA 5 cut(s) 269, 418, 728, 865, 1344
AleI CACNNNNGTG 1 cut(s) 1152
AluBI AGCT 5 cut(s) 249, 562, 724, 1021, 1090
AluI AGCT 5 cut(s) 249, 562, 724, 1021, 1090
Alw21I GWGCWC 2 cut(s) 251, 926
Alw26I GTCTC 1 cut(s) 150
Alw44I GTGCAC 1 cut(s) 922
AlwI GGATC 3 cut(s) 859, 1160, 1173
Ama87I CYCGRG 1 cut(s) 553
AoxI GGCC 4 cut(s) 421, 656, 1125, 1234
ApaLI GTGCAC 1 cut(s) 922
ApoI RAATTY 2 cut(s) 598, 613
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 2 cut(s) 288, 1215
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 1 cut(s) 656
AsuC2I CCSGG 3 cut(s) 153, 1151, 1190
AvaI CYCGRG 1 cut(s) 553
BaeGI GKGCMC 1 cut(s) 926
BamHI GGATCC 1 cut(s) 1165
BanI GGYRCC 1 cut(s) 356
BanII GRGCYC 2 cut(s) 251, 1018
Bbv12I GWGCWC 2 cut(s) 251, 926
BccI CCATC 4 cut(s) 347, 911, 1074, 1117
BceAI ACGGC 2 cut(s) 891, 1249
BclI TGATCA 1 cut(s) 964
BcnI CCSGG 3 cut(s) 153, 1151, 1190
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 1 cut(s) 1360
BfuAI ACCTGC 1 cut(s) 619
BglII AGATCT 1 cut(s) 949
Bme1390I CCNGG 3 cut(s) 153, 1151, 1190
BmeT110I CYCGRG 1 cut(s) 553
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 5 cut(s) 358, 816, 1015, 1167, 1194
BmrFI CCNGG 3 cut(s) 153, 1151, 1190
BmsI GCATC 7 cut(s) 295, 317, 339, 477, 509, 594, 1010
BplI GAGNNNNNCTC 2 cut(s) 34, 66
BpmI CTGGAG 1 cut(s) 1106
BpuEI CTTGAG 2 cut(s) 274, 880
BpuMI CCSGG 3 cut(s) 153, 1151, 1190
BsaBI GATNNNNATC 2 cut(s) 101, 856
BsaHI GRCGYC 1 cut(s) 993
BsaJI CCNNGG 2 cut(s) 375, 1150
BsaWI WCCGGW 1 cut(s) 1136
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 318, 497, 1080
Bse118I RCCGGY 1 cut(s) 529
Bse1I ACTGG 1 cut(s) 1124
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 2 cut(s) 101, 856
BseDI CCNNGG 2 cut(s) 375, 1150
BseGI GGATG 7 cut(s) 308, 310, 524, 922, 1001, 1085, 1128
BseJI GATNNNNATC 2 cut(s) 101, 856
BseLI CCNNNNNNNGG 4 cut(s) 175, 318, 497, 1080
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 79, 554
BseNI ACTGG 1 cut(s) 1124
BseRI GAGGAG 1 cut(s) 859
BseSI GKGCMC 1 cut(s) 926
BseX3I CGGCCG 1 cut(s) 1234
BseYI CCCAGC 1 cut(s) 1017
BsgI GTGCAG 1 cut(s) 647
Bsh1285I CGRYCG 1 cut(s) 1237
BshFI GGCC 4 cut(s) 423, 658, 1127, 1236
BshNI GGYRCC 1 cut(s) 356
BsiEI CGRYCG 1 cut(s) 1237
BsiHKAI GWGCWC 2 cut(s) 251, 926
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 5 cut(s) 153, 530, 1137, 1150, 1190
BslI CCNNNNNNNGG 4 cut(s) 175, 318, 497, 1080
BsmAI GTCTC 1 cut(s) 150
BsmI GAATGC 2 cut(s) 1190, 1217
BsnI GGCC 4 cut(s) 423, 658, 1127, 1236
BsoBI CYCGRG 1 cut(s) 553
Bsp1286I GDGCHC 3 cut(s) 251, 926, 1018
Bsp143I GATC 6 cut(s) 851, 857, 949, 964, 1129, 1165
BspACI CCGC 3 cut(s) 480, 677, 1007
BspANI GGCC 4 cut(s) 423, 658, 1127, 1236
BspCNI CTCAG 2 cut(s) 78, 555
BspHI TCATGA 1 cut(s) 243
BspLI GGNNCC 5 cut(s) 358, 816, 1015, 1167, 1194
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 3 cut(s) 859, 1160, 1173
BspQI GCTCTTC 1 cut(s) 1040
BspT107I GGYRCC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 529
BsrI ACTGG 1 cut(s) 1124
BssAI RCCGGY 1 cut(s) 529
BssECI CCNNGG 2 cut(s) 375, 1150
BssMI GATC 6 cut(s) 851, 857, 949, 964, 1129, 1165
BssNI GRCGYC 1 cut(s) 993
BssT1I CCWWGG 1 cut(s) 375
Bst4CI ACNGT 3 cut(s) 327, 1052, 1282
Bst6I CTCTTC 4 cut(s) 35, 86, 630, 1040
BstACI GRCGYC 1 cut(s) 993
BstC8I GCNNGC 3 cut(s) 531, 609, 1092
BstDEI CTNAG 2 cut(s) 65, 563
BstF5I GGATG 7 cut(s) 308, 310, 524, 922, 1001, 1085, 1128
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 6 cut(s) 854, 860, 952, 967, 1132, 1168
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 6 cut(s) 851, 857, 949, 964, 1129, 1165
BstMCI CGRYCG 1 cut(s) 1237
BstMWI GCNNNNNNNGC 3 cut(s) 218, 530, 884
BstNSI RCATGY 1 cut(s) 1033
BstSCI CCNGG 3 cut(s) 151, 1149, 1188
BstSLI GKGCMC 1 cut(s) 926
BstX2I RGATCY 2 cut(s) 949, 1165
BstYI RGATCY 2 cut(s) 949, 1165
BstZI CGGCCG 1 cut(s) 1234
BsuRI GGCC 4 cut(s) 423, 658, 1127, 1236
BtsCI GGATG 7 cut(s) 308, 310, 524, 922, 1001, 1085, 1128
BtsI GCAGTG 2 cut(s) 216, 918
BtsIMutI CAGTG 3 cut(s) 216, 918, 1117
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 3 cut(s) 531, 609, 1092
CciI TCATGA 1 cut(s) 243
CfoI GCGC 1 cut(s) 524
Cfr10I RCCGGY 1 cut(s) 529
Cfr13I GGNCC 1 cut(s) 656
CseI GACGC 2 cut(s) 798, 1153
Csp6I GTAC 1 cut(s) 542
CviAII CATG 6 cut(s) 22, 244, 484, 1010, 1030, 1039
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 2 cut(s) 65, 563
DpnI GATC 6 cut(s) 853, 859, 951, 966, 1131, 1167
DpnII GATC 6 cut(s) 851, 857, 949, 964, 1129, 1165
EaeI YGGCCR 2 cut(s) 1125, 1234
EagI CGGCCG 1 cut(s) 1234
Eam1104I CTCTTC 4 cut(s) 35, 86, 630, 1040
EarI CTCTTC 4 cut(s) 35, 86, 630, 1040
Ecl136II GAGCTC 1 cut(s) 249
EclXI CGGCCG 1 cut(s) 1234
Eco130I CCWWGG 1 cut(s) 375
Eco147I AGGCCT 1 cut(s) 423
Eco24I GRGCYC 2 cut(s) 251, 1018
Eco52I CGGCCG 1 cut(s) 1234
Eco53kI GAGCTC 1 cut(s) 249
Eco57I CTGAAG 2 cut(s) 408, 966
Eco88I CYCGRG 1 cut(s) 553
EcoICRI GAGCTC 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 656
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 2 cut(s) 251, 1018
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 6 cut(s) 25, 247, 487, 1013, 1033, 1042
FatI CATG 6 cut(s) 21, 243, 483, 1009, 1029, 1038
FauI CCCGC 1 cut(s) 1014
FauNDI CATATG 1 cut(s) 370
FbaI TGATCA 1 cut(s) 964
FokI GGATG 7 cut(s) 295, 317, 531, 929, 988, 1092, 1135
FriOI GRGCYC 2 cut(s) 251, 1018
FspBI CTAG 1 cut(s) 1360
FspI TGCGCA 1 cut(s) 523
GlaI GCGC 1 cut(s) 523
GsaI CCCAGC 1 cut(s) 1021
GsuI CTGGAG 1 cut(s) 1106
HaeIII GGCC 4 cut(s) 423, 658, 1127, 1236
HapII CCGG 5 cut(s) 153, 530, 1137, 1150, 1190
HgaI GACGC 2 cut(s) 798, 1153
HhaI GCGC 1 cut(s) 524
Hin1I GRCGYC 1 cut(s) 993
Hin1II CATG 6 cut(s) 25, 247, 487, 1013, 1033, 1042
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HincII GTYRAC 2 cut(s) 477, 831
HindII GTYRAC 2 cut(s) 477, 831
HindIII AAGCTT 1 cut(s) 722
HinfI GANTC 6 cut(s) 97, 158, 278, 592, 868, 1340
HpaI GTTAAC 1 cut(s) 477
HpaII CCGG 5 cut(s) 153, 530, 1137, 1150, 1190
Hpy166II GTNNAC 6 cut(s) 30, 395, 477, 831, 924, 1272
Hpy188I TCNGA 9 cut(s) 96, 170, 283, 304, 439, 597, 736, 873, 946
Hpy8I GTNNAC 6 cut(s) 30, 395, 477, 831, 924, 1272
Hpy99I CGWCG 2 cut(s) 500, 877
HpyAV CCTTC 9 cut(s) 138, 142, 299, 309, 312, 383, 640, 647, 667
HpyCH4III ACNGT 3 cut(s) 327, 1052, 1282
HpyCH4IV ACGT 5 cut(s) 410, 833, 926, 993, 1347
HpyCH4V TGCA 9 cut(s) 221, 308, 330, 607, 628, 924, 1001, 1094, 1265
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 530, 884
HpyF3I CTNAG 2 cut(s) 65, 563
HpySE526I ACGT 5 cut(s) 410, 833, 926, 993, 1347
Hsp92I GRCGYC 1 cut(s) 993
Hsp92II CATG 6 cut(s) 25, 247, 487, 1013, 1033, 1042
HspAI GCGC 1 cut(s) 522
KroI GCCGGC 1 cut(s) 529
KroNI GCCGGC 1 cut(s) 531
Ksp22I TGATCA 1 cut(s) 964
KspAI GTTAAC 1 cut(s) 477
Kzo9I GATC 6 cut(s) 851, 857, 949, 964, 1129, 1165
LguI GCTCTTC 1 cut(s) 1040
LmnI GCTCC 4 cut(s) 254, 814, 1013, 1087
LweI GCATC 7 cut(s) 295, 317, 339, 477, 509, 594, 1010
MaeI CTAG 1 cut(s) 1360
MaeII ACGT 5 cut(s) 410, 833, 926, 993, 1347
MaeIII GTNAC 5 cut(s) 178, 257, 568, 880, 1244
MalI GATC 6 cut(s) 853, 859, 951, 966, 1131, 1167
MboI GATC 6 cut(s) 851, 857, 949, 964, 1129, 1165
MflI RGATCY 2 cut(s) 949, 1165
MhlI GDGCHC 3 cut(s) 251, 926, 1018
MluCI AATT 2 cut(s) 598, 613
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 4 cut(s) 336, 462, 759, 896
Mph1103I ATGCAT 1 cut(s) 310
MroNI GCCGGC 1 cut(s) 529
MroXI GAANNNNTTC 2 cut(s) 288, 1215
MseI TTAA 2 cut(s) 476, 929
MslI CAYNNNNRTG 2 cut(s) 221, 1152
MspI CCGG 5 cut(s) 153, 530, 1137, 1150, 1190
MspR9I CCNGG 3 cut(s) 153, 1151, 1190
Mva1269I GAATGC 2 cut(s) 1190, 1217
MwoI GCNNNNNNNGC 3 cut(s) 218, 530, 884
NaeI GCCGGC 1 cut(s) 531
NciI CCSGG 3 cut(s) 153, 1151, 1190
NdeI CATATG 1 cut(s) 370
NdeII GATC 6 cut(s) 851, 857, 949, 964, 1129, 1165
NgoMIV GCCGGC 1 cut(s) 529
NlaIII CATG 6 cut(s) 25, 247, 487, 1013, 1033, 1042
NlaIV GGNNCC 5 cut(s) 358, 816, 1015, 1167, 1194
NmeAIII GCCGAG 1 cut(s) 1262
NmuCI GTSAC 3 cut(s) 257, 880, 1244
NsbI TGCGCA 1 cut(s) 523
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 1 cut(s) 1033
OliI CACNNNNGTG 1 cut(s) 1152
PagI TCATGA 1 cut(s) 243
PceI AGGCCT 1 cut(s) 423
PciSI GCTCTTC 1 cut(s) 1040
PcsI WCGNNNNNNNCGW 1 cut(s) 639
PctI GAATGC 2 cut(s) 1190, 1217
PdiI GCCGGC 1 cut(s) 531
PdmI GAANNNNTTC 2 cut(s) 288, 1215
PfeI GAWTC 5 cut(s) 97, 278, 592, 868, 1340
PflMI CCANNNNNTGG 1 cut(s) 1080
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Psp124BI GAGCTC 1 cut(s) 251
Psp1406I AACGTT 1 cut(s) 1347
PspFI CCCAGC 1 cut(s) 1017
PspN4I GGNNCC 5 cut(s) 358, 816, 1015, 1167, 1194
PspPI GGNCC 1 cut(s) 656
PsuI RGATCY 2 cut(s) 949, 1165
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
RseI CAYNNNNRTG 2 cut(s) 221, 1152
SacI GAGCTC 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 1040
SaqAI TTAA 2 cut(s) 476, 929
Sau3AI GATC 6 cut(s) 851, 857, 949, 964, 1129, 1165
Sau96I GGNCC 1 cut(s) 656
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 3 cut(s) 153, 1151, 1190
SduI GDGCHC 3 cut(s) 251, 926, 1018
SfaNI GCATC 7 cut(s) 295, 317, 339, 477, 509, 594, 1010
SmiMI CAYNNNNRTG 2 cut(s) 221, 1152
SmlI CTYRAG 2 cut(s) 253, 895
SmoI CTYRAG 2 cut(s) 253, 895
Sse9I AATT 2 cut(s) 598, 613
SseBI AGGCCT 1 cut(s) 423
SsiI CCGC 3 cut(s) 480, 677, 1007
SspMI CTAG 1 cut(s) 1360
SstI GAGCTC 1 cut(s) 251
StuI AGGCCT 1 cut(s) 423
StyD4I CCNGG 3 cut(s) 151, 1149, 1188
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 3 cut(s) 327, 1052, 1282
TaiI ACGT 5 cut(s) 413, 836, 929, 996, 1350
TaqI TCGA 6 cut(s) 161, 633, 642, 669, 1209, 1323
TasI AATT 2 cut(s) 598, 613
TfiI GAWTC 5 cut(s) 97, 278, 592, 868, 1340
Tru1I TTAA 2 cut(s) 476, 929
Tru9I TTAA 2 cut(s) 476, 929
TscAI CASTG 3 cut(s) 223, 918, 1124
TseFI GTSAC 3 cut(s) 257, 880, 1244
Tsp45I GTSAC 3 cut(s) 257, 880, 1244
TspDTI ATGAA 6 cut(s) 232, 281, 291, 524, 1027, 1266
TspGWI ACGGA 2 cut(s) 330, 471
TspRI CASTG 3 cut(s) 223, 918, 1124
Van91I CCANNNNNTGG 1 cut(s) 1080
VneI GTGCAC 1 cut(s) 922
XapI RAATTY 2 cut(s) 598, 613
XceI RCATGY 1 cut(s) 1033
XcmI CCANNNNNNNNNTGG 2 cut(s) 220, 1081
XmnI GAANNNNTTC 2 cut(s) 288, 1215
XspI CTAG 1 cut(s) 1360
ZraI GACGTC 1 cut(s) 994
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.