FvH4_5g35970

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004099
Physical Location & Seq
Reverse (-)
26365844 .. 26367852
2009 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35970.t1

Sequence Viewer

Length: 1419 bp
ATGCTTGACCTTGAAGAAACCATGGAAGTGAAAAGCAGTGCCAACAGACCTAAGAGCTTGAGCCCCTCAATAACTATACTCCTTATCGGTCTCTTTTCACTTTCTCTCATTCTCATCCTCTTTCATGGCTTTTCTTCACTCTCCACCTCTTCTGCTTCATCGGATCTTCATTTCTGGAAAAGTGAACTTGGTGCCAGCAACAACGATGCTCGTGATGATCTTAACTCGAGGCTCCGAGACTCGGTTACTTTCCTTCCTCTCAAAGACTTGAGGTTCACCAAAACTGCAATGGAGGGCAACACTTGGTTTATGAGCTCTTTCAGCGACACATCTGAAGAAAATGAATCCCAATACCTCTATTTCCCTTCACAAGCGTCAAAGGGAAGACTACTGTGCCTCAAGGGTCATGACCACAAAGATGGCACCGAAAACTCGTATGCCTTGGCATGGCCTCAAAGTCTACCAAACTCCACTACATTCTTGAAGGGCCTCACATTTGTGTCGGACACATACTATGATTATGTAAACTTGTGGCATGGACTCACTGCCATGGTCCCTTTTGTGGGATGGTCAATCAAGAACGGGTGTGTGAAGCCGACGCGATGGGTGCTGTTCCATTGGGGAGAGCTGAGGGAAAATATGGGATTGTGGCTTCAGAACCTAGTGGAAGCAAACTTTGGGAAAGTGATGATTGAACAAATGGGAAAAGGAGAAGGGCCATATTGTTTTGAGAAGGCTGTTGTGACGAGACATAATGTAGGGAAAATGGGGAAGGAGAAGAAGCTTCAAGTGTCTGACTTGTTGAGATGTAAAGCAAGAGTATTTTGTGGCATAAATCCGGCAGGTAGAGGGAAAGAGATTGGTGTCAGAGGGGAGCCAATCATAAGGCTAACTTTGTTAATGAGGAAAGCTTCAAGATCATTCAAGGATCCAACTGCAGTGATTAGTATATTTGCAAGGGAGTGTGCAATGGTGGATGGGTGCTTGTTGGAGGTTGTTCAGTCTGAAGACCTAAGTTTCTGCGATCAGGTTAAGGTCATGACCAACACAGATATTCTTGCATCCCCACATGGAGCTCAGCTAACAAACATGCTCTTCATGGATAGAAACAGTAGCATTATGGAATTCTTTCCTAAAGGGTGGTTAGAGCTTGCCGGGGCCGGCCAGTATGCCCATCACTGGATGGCAGATCAGTCCGGTATGAACCACCGAGGTGCATGGTGGGATCTTAATGCTGAAGAGGAATGCCCTGACCCTAAACAACAGAAAGAATGCTTTAACTTCTACAAAGATGGCCAGGTTGGTCACAACGAAACTTACTTTGCAGGGTGGGCTAGAACTGTTCTCAACCACGTTAGGATAAGCAAGCAGGAAGCCATGCAGAGTTCGCAGCAATTTTCAAATGCTTGCCAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF563

Protein Analysis

473

Amino Acids

53.26

Weight (kDa)

6.51

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_61 PF04577 269 - 373 1.3e-12 Glycosyltransferase 61
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 833
AccB1I GGYRCC 2 cut(s) 191, 422
AccI GTMKAC 1 cut(s) 460
AccII CGCG 1 cut(s) 601
AclWI GGATC 4 cut(s) 171, 923, 936, 1233
AcoI YGGCCR 2 cut(s) 1162, 1294
AcsI RAATTY 1 cut(s) 1124
AcuI CTGAAG 4 cut(s) 354, 638, 1026, 1257
AfiI CCNNNNNNNGG 5 cut(s) 241, 447, 562, 563, 1179
AgsI TTSAA 7 cut(s) 14, 484, 695, 788, 915, 925, 1401
AjnI CCWGG 1 cut(s) 1296
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AleI CACNNNNGTG 2 cut(s) 497, 1212
AluBI AGCT 8 cut(s) 57, 315, 628, 784, 911, 1076, 1081, 1150
AluI AGCT 8 cut(s) 57, 315, 628, 784, 911, 1076, 1081, 1150
Alw21I GWGCWC 2 cut(s) 317, 1078
Alw26I GTCTC 3 cut(s) 95, 231, 742
AlwI GGATC 4 cut(s) 171, 923, 936, 1233
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 6 cut(s) 449, 487, 716, 1158, 1162, 1294
ApeKI GCWGC 1 cut(s) 1390
ApoI RAATTY 1 cut(s) 1124
Asp700I GAANNNNTTC 1 cut(s) 1128
AspS9I GGNCC 4 cut(s) 487, 553, 716, 1158
AsuC2I CCSGG 1 cut(s) 1156
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 1 cut(s) 553
BalI TGGCCA 1 cut(s) 1296
BamHI GGATCC 1 cut(s) 928
BanI GGYRCC 2 cut(s) 191, 422
BanII GRGCYC 3 cut(s) 65, 317, 1078
BauI CACGAG 1 cut(s) 210
BbsI GAAGAC 2 cut(s) 391, 1014
Bbv12I GWGCWC 2 cut(s) 317, 1078
BbvCI CCTCAGC 1 cut(s) 629
BbvI GCAGC 1 cut(s) 1402
BccI CCATC 7 cut(s) 413, 561, 597, 971, 1177, 1182, 1286
BciT130I CCWGG 1 cut(s) 1298
BcnI CCSGG 1 cut(s) 1156
BcoDI GTCTC 3 cut(s) 95, 231, 742
BfaI CTAG 3 cut(s) 662, 1335, 1417
BfmI CTRYAG 1 cut(s) 936
BfuAI ACCTGC 1 cut(s) 833
BisI GCNGC 1 cut(s) 1391
BlpI GCTNAGC 1 cut(s) 1077
BlsI GCNGC 1 cut(s) 1392
Bme1390I CCNGG 2 cut(s) 1156, 1298
Bme18I GGWCC 1 cut(s) 553
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 4 cut(s) 487, 553, 716, 1158
BmiI GGNNCC 7 cut(s) 193, 233, 424, 555, 876, 930, 1159
BmrFI CCNGG 2 cut(s) 1156, 1298
BmsI GCATC 2 cut(s) 196, 1070
BpiI GAAGAC 2 cut(s) 391, 1014
Bpu10I CCTNAGC 1 cut(s) 629
Bpu1102I GCTNAGC 1 cut(s) 1077
BpuEI CTTGAG 3 cut(s) 79, 289, 383
BpuMI CCSGG 1 cut(s) 1156
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 5 cut(s) 21, 441, 549, 1155, 1210
BsaWI WCCGGW 1 cut(s) 1196
Bsc4I CCNNNNNNNGG 5 cut(s) 241, 447, 562, 563, 1179
Bse118I RCCGGY 1 cut(s) 1160
Bse1I ACTGG 2 cut(s) 1165, 1184
Bse3DI GCAATG 2 cut(s) 294, 975
BseBI CCWGG 1 cut(s) 1298
BseDI CCNNGG 5 cut(s) 21, 441, 549, 1155, 1210
BseGI GGATG 5 cut(s) 114, 572, 982, 1061, 1188
BseLI CCNNNNNNNGG 5 cut(s) 241, 447, 562, 563, 1179
BseMI GCAATG 2 cut(s) 294, 975
BseMII CTCAG 2 cut(s) 620, 1091
BseNI ACTGG 2 cut(s) 1165, 1184
BseXI GCAGC 1 cut(s) 1402
Bsh1236I CGCG 1 cut(s) 601
BshFI GGCC 6 cut(s) 451, 489, 718, 1160, 1164, 1296
BshNI GGYRCC 2 cut(s) 191, 422
BsiHKAI GWGCWC 2 cut(s) 317, 1078
BsiHKCI CYCGRG 1 cut(s) 226
BsiSI CCGG 4 cut(s) 839, 1155, 1161, 1197
BslFI GGGAC 1 cut(s) 539
BslI CCNNNNNNNGG 5 cut(s) 241, 447, 562, 563, 1179
BsmAI GTCTC 3 cut(s) 95, 231, 742
BsmFI GGGAC 1 cut(s) 539
BsmI GAATGC 2 cut(s) 1250, 1277
BsnI GGCC 6 cut(s) 451, 489, 718, 1160, 1164, 1296
Bso31I GGTCTC 1 cut(s) 95
BsoBI CYCGRG 1 cut(s) 226
Bsp1286I GDGCHC 3 cut(s) 65, 317, 1078
Bsp143I GATC 7 cut(s) 163, 217, 917, 928, 1024, 1189, 1225
Bsp1720I GCTNAGC 1 cut(s) 1077
Bsp19I CCATGG 2 cut(s) 21, 549
BspANI GGCC 6 cut(s) 451, 489, 718, 1160, 1164, 1296
BspCNI CTCAG 2 cut(s) 621, 1090
BspFNI CGCG 1 cut(s) 601
BspHI TCATGA 2 cut(s) 406, 1038
BspLI GGNNCC 7 cut(s) 193, 233, 424, 555, 876, 930, 1159
BspMAI CTGCAG 1 cut(s) 940
BspMI ACCTGC 1 cut(s) 833
BspPI GGATC 4 cut(s) 171, 923, 936, 1233
BspQI GCTCTTC 1 cut(s) 1100
BspT107I GGYRCC 2 cut(s) 191, 422
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 2 cut(s) 294, 975
BsrFI RCCGGY 1 cut(s) 1160
BsrI ACTGG 2 cut(s) 1165, 1184
BssAI RCCGGY 1 cut(s) 1160
BssECI CCNNGG 5 cut(s) 21, 441, 549, 1155, 1210
BssMI GATC 7 cut(s) 163, 217, 917, 928, 1024, 1189, 1225
BssSI CACGAG 1 cut(s) 210
BssT1I CCWWGG 3 cut(s) 21, 441, 549
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 1298
Bst4CI ACNGT 3 cut(s) 393, 1112, 1342
Bst6I CTCTTC 3 cut(s) 154, 1100, 1233
BstC8I GCNNGC 5 cut(s) 196, 1152, 1162, 1367, 1408
BstDEI CTNAG 4 cut(s) 51, 629, 1013, 1077
BstDSI CCRYGG 2 cut(s) 21, 549
BstF5I GGATG 5 cut(s) 114, 572, 982, 1061, 1188
BstFNI CGCG 1 cut(s) 601
BstKTI GATC 7 cut(s) 166, 220, 920, 931, 1027, 1192, 1228
BstMAI GTCTC 3 cut(s) 95, 231, 742
BstMBI GATC 7 cut(s) 163, 217, 917, 928, 1024, 1189, 1225
BstMWI GCNNNNNNNGC 4 cut(s) 321, 607, 1331, 1387
BstNI CCWGG 1 cut(s) 1298
BstNSI RCATGY 1 cut(s) 1093
BstSCI CCNGG 2 cut(s) 1154, 1296
BstSFI CTRYAG 1 cut(s) 936
BstUI CGCG 1 cut(s) 601
BstV1I GCAGC 1 cut(s) 1402
BstV2I GAAGAC 2 cut(s) 391, 1014
BstX2I RGATCY 3 cut(s) 163, 928, 1225
BstXI CCANNNNNNTGG 1 cut(s) 419
BstYI RGATCY 3 cut(s) 163, 928, 1225
BsuRI GGCC 6 cut(s) 451, 489, 718, 1160, 1164, 1296
BtgI CCRYGG 2 cut(s) 21, 549
BtgZI GCGATG 1 cut(s) 616
BtsCI GGATG 5 cut(s) 114, 572, 982, 1061, 1188
BtsI GCAGTG 3 cut(s) 43, 543, 945
BtsIMutI CAGTG 4 cut(s) 43, 543, 945, 1177
BveI ACCTGC 1 cut(s) 833
Cac8I GCNNGC 5 cut(s) 196, 1152, 1162, 1367, 1408
CciI TCATGA 2 cut(s) 406, 1038
Cfr10I RCCGGY 1 cut(s) 1160
Cfr13I GGNCC 4 cut(s) 487, 553, 716, 1158
CseI GACGC 2 cut(s) 363, 607
DdeI CTNAG 4 cut(s) 51, 629, 1013, 1077
DpnI GATC 7 cut(s) 165, 219, 919, 930, 1026, 1191, 1227
DpnII GATC 7 cut(s) 163, 217, 917, 928, 1024, 1189, 1225
EaeI YGGCCR 2 cut(s) 1162, 1294
Eam1104I CTCTTC 3 cut(s) 154, 1100, 1233
EarI CTCTTC 3 cut(s) 154, 1100, 1233
Ecl136II GAGCTC 2 cut(s) 315, 1076
Eco130I CCWWGG 3 cut(s) 21, 441, 549
Eco24I GRGCYC 3 cut(s) 65, 317, 1078
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 1 cut(s) 553
Eco53kI GAGCTC 2 cut(s) 315, 1076
Eco57I CTGAAG 4 cut(s) 354, 638, 1026, 1257
Eco88I CYCGRG 1 cut(s) 226
EcoICRI GAGCTC 2 cut(s) 315, 1076
EcoO109I RGGNCCY 1 cut(s) 487
EcoRI GAATTC 1 cut(s) 1124
EcoRII CCWGG 1 cut(s) 1296
EcoT14I CCWWGG 3 cut(s) 21, 441, 549
EcoT38I GRGCYC 3 cut(s) 65, 317, 1078
ErhI CCWWGG 3 cut(s) 21, 441, 549
FalI AAGNNNNNCTT 2 cut(s) 877, 909
FaqI GGGAC 1 cut(s) 539
FblI GTMKAC 1 cut(s) 460
Fnu4HI GCNGC 1 cut(s) 1391
FokI GGATG 5 cut(s) 101, 579, 989, 1048, 1195
FriOI GRGCYC 3 cut(s) 65, 317, 1078
FseI GGCCGGCC 1 cut(s) 1164
Fsp4HI GCNGC 1 cut(s) 1391
FspBI CTAG 3 cut(s) 662, 1335, 1417
GluI GCNGC 1 cut(s) 1391
HaeIII GGCC 6 cut(s) 451, 489, 718, 1160, 1164, 1296
HapII CCGG 4 cut(s) 839, 1155, 1161, 1197
HgaI GACGC 2 cut(s) 363, 607
HindIII AAGCTT 2 cut(s) 782, 909
HinfI GANTC 3 cut(s) 239, 344, 540
HpaII CCGG 4 cut(s) 839, 1155, 1161, 1197
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 4 cut(s) 185, 276, 461, 526
Hpy188I TCNGA 8 cut(s) 163, 236, 334, 505, 657, 796, 869, 1006
Hpy188III TCNNGA 7 cut(s) 175, 212, 407, 481, 577, 915, 1039
Hpy8I GTNNAC 4 cut(s) 185, 276, 461, 526
Hpy99I CGWCG 1 cut(s) 601
HpyAV CCTTC 6 cut(s) 263, 375, 478, 707, 727, 766
HpyCH4III ACNGT 3 cut(s) 393, 1112, 1342
HpyCH4IV ACGT 1 cut(s) 1353
HpyCH4V TGCA 8 cut(s) 287, 938, 956, 968, 1061, 1217, 1325, 1381
HpyF10VI GCNNNNNNNGC 4 cut(s) 321, 607, 1331, 1387
HpyF3I CTNAG 4 cut(s) 51, 629, 1013, 1077
HpySE526I ACGT 1 cut(s) 1353
KroI GCCGGC 1 cut(s) 1160
KroNI GCCGGC 1 cut(s) 1162
Kzo9I GATC 7 cut(s) 163, 217, 917, 928, 1024, 1189, 1225
LguI GCTCTTC 1 cut(s) 1100
LmnI GCTCC 3 cut(s) 237, 874, 1073
Lsp1109I GCAGC 1 cut(s) 1402
LweI GCATC 2 cut(s) 196, 1070
MaeI CTAG 3 cut(s) 662, 1335, 1417
MaeII ACGT 1 cut(s) 1353
MaeIII GTNAC 3 cut(s) 244, 742, 1304
MalI GATC 7 cut(s) 165, 219, 919, 930, 1026, 1191, 1227
MboI GATC 7 cut(s) 163, 217, 917, 928, 1024, 1189, 1225
MflI RGATCY 3 cut(s) 163, 928, 1225
MhlI GDGCHC 3 cut(s) 65, 317, 1078
MlsI TGGCCA 1 cut(s) 1296
MluCI AATT 2 cut(s) 1124, 1394
MluNI TGGCCA 1 cut(s) 1296
MlyI GAGTC 2 cut(s) 233, 534
MmeI TCCRAC 3 cut(s) 483, 956, 969
Mox20I TGGCCA 1 cut(s) 1296
MroNI GCCGGC 1 cut(s) 1160
MroXI GAANNNNTTC 1 cut(s) 1128
MscI TGGCCA 1 cut(s) 1296
MseI TTAA 5 cut(s) 222, 899, 1032, 1230, 1278
MslI CAYNNNNRTG 5 cut(s) 26, 417, 497, 548, 1212
Msp20I TGGCCA 1 cut(s) 1296
MspI CCGG 4 cut(s) 839, 1155, 1161, 1197
MspR9I CCNGG 2 cut(s) 1156, 1298
Mva1269I GAATGC 2 cut(s) 1250, 1277
MvaI CCWGG 1 cut(s) 1298
MvnI CGCG 1 cut(s) 601
MwoI GCNNNNNNNGC 4 cut(s) 321, 607, 1331, 1387
NaeI GCCGGC 1 cut(s) 1162
NciI CCSGG 1 cut(s) 1156
NcoI CCATGG 2 cut(s) 21, 549
NdeII GATC 7 cut(s) 163, 217, 917, 928, 1024, 1189, 1225
NgoMIV GCCGGC 1 cut(s) 1160
NlaIV GGNNCC 7 cut(s) 193, 233, 424, 555, 876, 930, 1159
NmuCI GTSAC 2 cut(s) 742, 1304
NspI RCATGY 1 cut(s) 1093
OliI CACNNNNGTG 2 cut(s) 497, 1212
PaeR7I CTCGAG 1 cut(s) 226
PagI TCATGA 2 cut(s) 406, 1038
PciSI GCTCTTC 1 cut(s) 1100
PctI GAATGC 2 cut(s) 1250, 1277
PdiI GCCGGC 1 cut(s) 1162
PdmI GAANNNNTTC 1 cut(s) 1128
PfeI GAWTC 1 cut(s) 344
PkrI GCNGC 1 cut(s) 1392
PleI GAGTC 2 cut(s) 233, 534
PpsI GAGTC 2 cut(s) 233, 534
Psp124BI GAGCTC 2 cut(s) 317, 1078
Psp6I CCWGG 1 cut(s) 1296
PspGI CCWGG 1 cut(s) 1296
PspN4I GGNNCC 7 cut(s) 193, 233, 424, 555, 876, 930, 1159
PspPI GGNCC 4 cut(s) 487, 553, 716, 1158
PspXI VCTCGAGB 1 cut(s) 226
PstI CTGCAG 1 cut(s) 940
PsuI RGATCY 3 cut(s) 163, 928, 1225
RigI GGCCGGCC 1 cut(s) 1164
RseI CAYNNNNRTG 5 cut(s) 26, 417, 497, 548, 1212
SacI GAGCTC 2 cut(s) 317, 1078
SapI GCTCTTC 1 cut(s) 1100
SaqAI TTAA 5 cut(s) 222, 899, 1032, 1230, 1278
SatI GCNGC 1 cut(s) 1391
Sau3AI GATC 7 cut(s) 163, 217, 917, 928, 1024, 1189, 1225
Sau96I GGNCC 4 cut(s) 487, 553, 716, 1158
SchI GAGTC 2 cut(s) 233, 534
ScrFI CCNGG 2 cut(s) 1156, 1298
SduI GDGCHC 3 cut(s) 65, 317, 1078
SfaNI GCATC 2 cut(s) 196, 1070
SfcI CTRYAG 1 cut(s) 936
Sfr274I CTCGAG 1 cut(s) 226
SinI GGWCC 1 cut(s) 553
SlaI CTCGAG 1 cut(s) 226
SmiMI CAYNNNNRTG 5 cut(s) 26, 417, 497, 548, 1212
SmlI CTYRAG 4 cut(s) 58, 226, 268, 398
SmoI CTYRAG 4 cut(s) 58, 226, 268, 398
Sse9I AATT 2 cut(s) 1124, 1394
SspMI CTAG 3 cut(s) 662, 1335, 1417
SstI GAGCTC 2 cut(s) 317, 1078
StyD4I CCNGG 2 cut(s) 1154, 1296
StyI CCWWGG 3 cut(s) 21, 441, 549
TaaI ACNGT 3 cut(s) 393, 1112, 1342
TaiI ACGT 1 cut(s) 1356
TaqI TCGA 1 cut(s) 227
TaqII GACCGA 1 cut(s) 77
TasI AATT 2 cut(s) 1124, 1394
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 5 cut(s) 222, 899, 1032, 1230, 1278
Tru9I TTAA 5 cut(s) 222, 899, 1032, 1230, 1278
TscAI CASTG 4 cut(s) 43, 550, 945, 1184
TseFI GTSAC 2 cut(s) 742, 1304
TseI GCWGC 1 cut(s) 1390
Tsp45I GTSAC 2 cut(s) 742, 1304
TspDTI ATGAA 6 cut(s) 113, 147, 158, 357, 1087, 1217
TspRI CASTG 4 cut(s) 43, 550, 945, 1184
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 1 cut(s) 1124
XceI RCATGY 1 cut(s) 1093
XcmI CCANNNNNNNNNTGG 1 cut(s) 286
XhoI CTCGAG 1 cut(s) 226
XmiI GTMKAC 1 cut(s) 460
XmnI GAANNNNTTC 1 cut(s) 1128
XspI CTAG 3 cut(s) 662, 1335, 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.