Rorug03G0330300

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004099
Physical Location & Seq
Forward (+)
38340102 .. 38354866
14765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0330300.1

Sequence Viewer

Length: 4923 bp
ATGGGCAAAGCTGTCATCTCCGACGACGAGGATGAGGTCGAGCTCGACGAGGAGGATAGGGAACCCCTCGACGGGGACACAGTCGACCAGGACGGCGGCGGCGGCGGCGACGAAGATGAGGACGAGGAAGACGAAGGGCCAGATGAATATGAGAAGGATGATTTTATCGTAGATGATGTTGATGAAGAAGATGATCAGGAGGAAGAAGAGAGGGCCGACAGTGATGAGGAGAGGCAGAAGAAGAAGAAAAGAAAGAAAAAAGAGGAGTATGTTCTTGATGAAGATGACTACGAGCTCCTGGAGGACAATAATGTGATTGCCCCGCGCAGAAAGGGGAAATTTAAGCGGCTGAAAAAAGCTCAGAGGCATGCTCAGGGGGACCTTGGTGGATTATCTGATGAGGAGGAGTTTCCTGGTACTGGTAAAGGGGGAAGAACTGCAGAGGAGAAGCTTAAGTTCTCATTATTTGGTGGTGATGATGAAGGCCCTCCACTTGAGGACATTGCTGAAGAGGAGGAACCAGCAGAAGCGGAAGATGACGGAGAAGATGAAATGGCAGACTTTATAGTCGACGAAGAATTTGATGAGGCTGGTGTTCCTGTCAGACAGAAGAAGCTTAAGAAAAAGAAATCCAGGCAGGCACCAGGAGTATCTTCATCTGCTCTGCAGGAAGCCCATGACATATTCGGTGATGTTGATGAATTACTGCAGCTGCGTAAGCAAGGTTTGGATTCTAGTGAATGGAAGGAAAGGAGGCTTGAAGATGAATTTGAACCAATTGTTCTTTCGGAAAAGTATATGACAGAGAAGGATGACCAGATTCGGGAGATTGATGTCCCAGAAAGAATTCAGGTATATGAGGAAAGCACTGGTTTTCCTCCACTTGATGAAAAGAGCATAGATGATGAGAGTACCTGGATATTCAATCAACTTGCAAGTGGTACAGTTCCTTTTTTTGGCAAGACAGGCCTGGGGAATTCCATAAGCAGGGACGACATCATTGGATTCTTGAACTTGCACCATGTGCAGAAATTGGATGTTCCTTTTATTGCAATGTATCGGAAAGAGGAGTGCCTTAGTATATTGAAAGATCCTGAACACATGGACATGGATGATCAAAATGAGAAAGCATCCACACTAAAGTGGCACAAGGTACTTTGGTCCATTCAGGACTTGCATAGAAAATGGTTGCTGCTTCAGAAGAGGAAAAGTGCTCTGCAATCTTATTACGAAAAGCGGTTTGATGAAGAATCTCGACGCATATATGATGAGACCAGACTAACTTTAAATCAGCAACTTTTTGAATCAATTATGAAGTCACTAAAGGCTGCAGAATCAGAGAGAGAAGTTGATGATGTGGACACCAAGTTTAACTTGCATTTCCCTGCTGGTGAGATAGGTGTGGATGAGGGCCAATATAAGAGGCCTAAGAGGAAGTCCCTTTATAGTATCTGCAGTAAGGCTGGCTTGTGGGAGGTTGCCAGCAAGTTTGGTTACACCTCTGAACAGTTTGGTTTGCAATTGTCACTAGAGAAAATGAGAATGGATGAGCTGGAGGATGCGAAAGAGACGCCAGAGGAATTGGCTTCAAACTTCACATGTGCAATGTTTGAGACACCTCAGGAAGTGCTCAAAGGAGCCAGGCATATGGCTGCTGTTGAGATAAGCTGTGAGCCATGTGTCAGGAAGTATGTTAGGAGCAACTACTTGGATATGGTTGAGTTGTCCACCAGCCCAACACCAGACGGAAATACTGCAATTGAAGCTTTTCATCAGTTTGCAGGGGTGAAGTGGTTGCAAAGAAAGCCATTAAATAGATTTGGGGATGCACAATGGCTGCTTATCCAGAAGGCTGAAGAGGAGAAGCTTCTTCAAGTTACTATTAAGCTACCGGAAGACAGACTAAACAAGTTGATGAGCGACTTTAATGAGTACTATCTTAGTGATGGTGTTAGTAAATCTGCACAACTGTGGAATGAGCAGCGGAAGTTGATATTGCAGGATGCTCTTTTCAATTTTCTTCTACCTTCAATGGAGAAAGAAGCAAGATCCTTGTTGACTAGTAGGGCAAAAAACTGGTTATTGATGGAATACGGAAAGGTTTTGTGGAATAAAGTCTCTGTGGGACCATATCAACGGAAAGAGAATGACATTAGTTCAGACGATGAAGCTGCACCCAGGGTCATGGCTTGCTGTTGGGGCCCTGGGAAGCCAGCAACCACTTTTGTTATGTTAGATTCATCGGGGGAGGTGCTAGATGTGCTTTACACGGGATCTCTCACCTTACGCTCCCAGAACGTTAACGATCAGCAACGTAAGAAAAATGACCAAGAACGTGTTTTGAAGTTCATGACTGATCACCAACCACATGTGGCTGTTCTAGGAGCTGTTAATTTGTCTTGTGTTCGCCTGAAAGAAGATATTTACGAGATAATCTTTAAGATGGTGGAGGAGAATCCTAGAGATGTTGGACAGGATATGGATGGGCTAAGCATTGTCTATGGAGATGAATCTTTGGCTCGCCTGTATGAAAACTCTCGCATTTCATCTGATCAGCTACCTGCACAATCAGGCATTGTGAAGCGTGCTGTTGCTCTGGGGCGTTACCTTCAAAATCCATTGGCAATGGTCGCCACTCTTTGTGGGCCAGGAAGAGAAATCTTATCTTGGAAACTCAATCCTTTGGAGAACTTTCTCACCCCAGATGAGAAGTATGCAATGATTGAACAAGTCATGGTAGATGTTACAAACCAAGTAGGCCTCGACATAAATTTGGCTATAAGCCACGAATGGTTATTTGCACCTTTGCAATTCATTTCTGGGCTCGGACCTAGAAAAGCAGCATTTTTGCAGCGATCGTTGGTAAGATCTGGTGCAATATTTACTCGGAAGGACTTTTTGACAGCACATGGGTTAAGTAAAAAGGTGTTTGTTAATGCAGTTGGTTTCTTGCGTGTTCGGCGCAGTGGGCTGGCTGCTAGCAGCAGCCAATTTATTGATCTGTTGGATGATACAAGAATTCATCCAGAATCCTATATTCTTGCCCAAGAATTGGCCAAAGATGTTTTTGAAGTTGATGGTGGCAATGATGATGAGGATGCTATGGAGATGGCTATAGAACATGTTAGGGATCGGCCTGCTTATCTAAAAAATCTTGATGTTGAAGCGTATGCTAAAAGCAAAGAGCGTGAGAATAAGATACAAACATTTTGTGACATCAGGAGAGAATTAATACAGGGTTTTCAGGATTGGCGTAAGAAATATGAAGAACTAAGTCAAGATGAAGAATTCTATATGATTTCAGGTGAAACTGAAGATACCCTTGCTGAAGGAAGAATTGTGCTGGCCACAGTTCGCAGGGTACAAGCTCAAAGGGCAATATGTGCACTTGAATCTGGACTGACTGGTATGCTTATGAAAGAAGACTATTCAGATGATTCAAGGGATATTTCTGACTTATCTGAGAGACTGAATGAGGGTGACATTCTCTCGTGCAAGATCAAATCTATTCAAAAGAATAGGTATCAGGTGTTCCTAGTTTGTAGAGAGAGTGAAATGAGAAATAATCGAAATCAAAATATTCAGCATCTCGATAACTATTACCATGAAGACAGGAGAAGTCTACAGACAGAGCAAGAAAAAGCTCGTAAGGAGAAAGAGCTTGCGAAAAAACATTTCAAGCCAAGGATGATTGTTCATCCTCGCTTCCAGAATATTACGGCTGATGAAGCAATGAAGTTCTTATCTGATAAAGATCCTGGGGAAAGTATTATTCGTCCTAGTTCCCGTGGGCCTTCGTATTTGACCTTAACTCTGAAAGTTTACGATGGAGTTTATGCTCACAAAGACGTAGTTGAAGGTGGAAAGGAACACAAGGATATCACTAGCTTACTCCGTATCGGAAAGACTCTGAAAATTGGAGAGGACACTTTTGAAGATTTAGATGAGGTAATGGATCGTTATGTTGATCCCCTGGTGTCTCACTTGAAGGCAATGCTAAATTATCGCAAATTCCGACGGGGCACAAAAGCAGAGGTTGATGAACTGTTGAAGATTGAAAAATTGGAGTTTCCTATGAGGATTGTTTATTGTTTCGGGATATCACATGAACATCCAGGGACTTTCATACTGACCTATATACGGAGTACAAATCCACACCATGAGTATGTTGGGCTGTATCCTAAGGGGTTCAAGTTCAGAAAAAGGATGTTTGAGAGCATTGATCGGCTTGTAGCACATTTTCAGAAGTATATTGATGAGCCTCAACATGAGTCCGGACCATCAATTAGATCAGTTGCTGCTATGGTGCCCATGCGAAGCCCTGCAACTGGGGGTTCTTCGGGTGCATCCGCAGGATGGGGTGGTTCAAACAATGAGGGTGGTTGGAGAGGCCAGTCGTTTGACAGGGATAGGTCTTCTACACCGAGTTCGCGAACAGGCAGAAATGATTACAGAAATGGTGGTGGTCGAGATGGACATCCAAGTGGCCTACCTAGACCATATGGAGGGCGCGGTCGTGGTCGAGGAACATATAACGATAGCCGAGGCAATAATGAGAGACATGATTCAGGTTTTGATGGTCCTACATGGGGTTCAGATACCAAGGATGGCAATGATGGCTTGGGCAACTTTCCAGGTGCAAAGGTTCAAAACTCACCTGGCAGGGAAGCTTTCCCAGGTGGCTGGGGTACAGGTGGAAGTGGTAGTGGTAGTGGAGGAGGCAGCAGCTGGGCTGGTGGTGGTGGAAGTGGCTGGGGTCAAGAAAGTGGTGGTGGTTCAGGAGGATGGGGTGGGGCTGCTGCAAATGATGCTGCTGCTGATAATGAGCCTTCAGGATGGGGTTCTGACTCGAAAAAGAAGGGTTCGGGTGATGGTACTTCAGGATGGGGTTCTCAGAGTGGAGGTGGCGGTGGTGGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF563

Protein Analysis

1640

Amino Acids

184.93

Weight (kDa)

4.99

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT6_acidic PF14632 37 - 122 5e-16 Acidic N-terminal SPT6
HTH_44 PF14641 295 - 412 2.6e-06 Helix-turn-helix DNA-binding domain of SPT6
Tex_central_region PF22706 521 - 690 1.1e-06 Tex central region-like
YqgF PF14639 725 - 881 2e-17 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 885 - 986 1.1e-20 Helix-hairpin-helix motif
Spt6_S1 PF21710 1097 - 1185 1.2e-08 Spt6, S1/OB domain
SH2_2 PF14633 1201 - 1416 6.8e-69 SH2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 566
Acc36I ACCTGC 1 cut(s) 2569
AccB1I GGYRCC 2 cut(s) 640, 4306
AccB7I CCANNNNNTGG 1 cut(s) 3052
AccI GTMKAC 3 cut(s) 84, 570, 3622
AccII CGCG 3 cut(s) 325, 4432, 4512
AccIII TCCGGA 1 cut(s) 4274
AclI AACGTT 1 cut(s) 2298
AclWI GGATC 7 cut(s) 1085, 2043, 2281, 3138, 3749, 3962, 3963
AcoI YGGCCR 2 cut(s) 3054, 3345
AcsI RAATTY 9 cut(s) 338, 578, 767, 846, 976, 2770, 3018, 3287, 4010
AcuI CTGAAG 7 cut(s) 528, 1181, 1875, 3333, 3348, 4815, 4863
AcyI GRCGYC 1 cut(s) 1571
AdeI CACNNNGTG 1 cut(s) 1024
AfaI GTAC 9 cut(s) 418, 913, 943, 1155, 1934, 3363, 4147, 4690, 4876
AflII CTTAAG 2 cut(s) 452, 617
AflIII ACRYGT 4 cut(s) 1598, 2335, 2368, 3121
AhlI ACTAGT 1 cut(s) 2060
AjuI GAANNNNNNNTTGG 2 cut(s) 769, 801
AleI CACNNNNGTG 2 cut(s) 1141, 1969
Alw21I GWGCWC 5 cut(s) 45, 297, 1216, 1632, 3388
Alw26I GTCTC 7 cut(s) 1265, 1562, 1607, 2122, 3460, 3984, 4552
Alw44I GTGCAC 1 cut(s) 3384
AlwI GGATC 7 cut(s) 1085, 2043, 2281, 3138, 3749, 3962, 3963
AlwNI CAGNNNCTG 2 cut(s) 4298, 4728
Aor13HI TCCGGA 1 cut(s) 4274
ApaI GGGCCC 1 cut(s) 2204
ApaLI GTGCAC 1 cut(s) 3384
ApoI RAATTY 9 cut(s) 338, 578, 767, 846, 976, 2770, 3018, 3287, 4010
ArsI GACNNNNNNTTYG 2 cut(s) 563, 595
AseI ATTAAT 1 cut(s) 3230
Asp700I GAANNNNTTC 2 cut(s) 846, 1767
AspLEI GCGC 3 cut(s) 327, 2964, 4512
AsuNHI GCTAGC 1 cut(s) 2978
AvaII GGWCC 6 cut(s) 379, 1161, 2126, 2828, 4277, 4580
AxyI CCTNAGG 2 cut(s) 1620, 4182
BaeGI GKGCMC 4 cut(s) 2204, 3388, 4025, 4311
BaeI ACNNNNGTAYC 2 cut(s) 1145, 1178
BalI TGGCCA 2 cut(s) 3056, 3347
BanI GGYRCC 2 cut(s) 640, 4306
BanII GRGCYC 4 cut(s) 45, 297, 2204, 2826
BauI CACGAG 1 cut(s) 3490
BbsI GAAGAC 5 cut(s) 135, 1902, 3429, 3615, 4407
Bbv12I GWGCWC 5 cut(s) 45, 297, 1216, 1632, 3388
BceAI ACGGC 2 cut(s) 109, 3735
BcgI CGANNNNNNTGC 2 cut(s) 2153, 2187
BciVI GTATCC 1 cut(s) 4188
BclI TGATCA 4 cut(s) 193, 1114, 2356, 2551
BcoDI GTCTC 7 cut(s) 1265, 1562, 1607, 2122, 3460, 3984, 4552
BcuI ACTAGT 1 cut(s) 2060
BfmI CTRYAG 7 cut(s) 438, 665, 707, 1329, 1453, 3114, 3623
BfrI CTTAAG 2 cut(s) 452, 617
BfuAI ACCTGC 1 cut(s) 2569
BfuI GTATCC 1 cut(s) 4188
BglII AGATCT 1 cut(s) 2867
BlpI GCTNAGC 1 cut(s) 2489
BmcAI AGTACT 1 cut(s) 1934
Bme18I GGWCC 6 cut(s) 379, 1161, 2126, 2828, 4277, 4580
BmiI GGNNCC 9 cut(s) 63, 380, 519, 642, 1639, 2127, 2201, 2202, 4308
BmrI ACTGGG 1 cut(s) 4338
BmsI GCATC 8 cut(s) 1139, 1549, 1816, 1993, 3088, 3595, 4355, 4798
BmtI GCTAGC 1 cut(s) 2982
BmuI ACTGGG 1 cut(s) 4338
BpiI GAAGAC 5 cut(s) 135, 1902, 3429, 3615, 4407
BplI GAGNNNNNCTC 2 cut(s) 355, 387
BpmI CTGGAG 2 cut(s) 320, 1574
Bpu10I CCTNAGC 1 cut(s) 372
Bpu1102I GCTNAGC 1 cut(s) 2489
BpuEI CTTGAG 1 cut(s) 515
BsaBI GATNNNNATC 2 cut(s) 3753, 4476
BsaHI GRCGYC 1 cut(s) 1571
BsaI GGTCTC 1 cut(s) 1265
BsaWI WCCGGW 2 cut(s) 1891, 4274
Bse1I ACTGG 6 cut(s) 424, 874, 2081, 3409, 4333, 4393
Bse21I CCTNAGG 2 cut(s) 1620, 4182
Bse3DI GCAATG 9 cut(s) 501, 1059, 1611, 2631, 2724, 3091, 3738, 3999, 4618
Bse8I GATNNNNATC 2 cut(s) 3753, 4476
BseAI TCCGGA 1 cut(s) 4274
BseJI GATNNNNATC 2 cut(s) 3753, 4476
BseMI GCAATG 9 cut(s) 501, 1059, 1611, 2631, 2724, 3091, 3738, 3999, 4618
BseMII CTCAG 5 cut(s) 374, 386, 1634, 3453, 4907
BseNI ACTGG 6 cut(s) 424, 874, 2081, 3409, 4333, 4393
BseSI GKGCMC 4 cut(s) 2204, 3388, 4025, 4311
BseYI CCCAGC 3 cut(s) 4683, 4728, 4752
BsgI GTGCAG 4 cut(s) 1046, 1947, 2157, 2547
Bsh1236I CGCG 3 cut(s) 325, 4432, 4512
Bsh1285I CGRYCG 2 cut(s) 2858, 4516
BshNI GGYRCC 2 cut(s) 640, 4306
BsiEI CGRYCG 2 cut(s) 2858, 4516
BsiHKAI GWGCWC 5 cut(s) 45, 297, 1216, 1632, 3388
BsiSI CCGG 2 cut(s) 1892, 4275
BslFI GGGAC 7 cut(s) 89, 392, 821, 1004, 1423, 2139, 4132
BsmAI GTCTC 7 cut(s) 1265, 1562, 1607, 2122, 3460, 3984, 4552
BsmBI CGTCTC 1 cut(s) 1562
BsmFI GGGAC 7 cut(s) 89, 392, 821, 1004, 1423, 2139, 4132
Bso31I GGTCTC 1 cut(s) 1265
Bsp120I GGGCCC 1 cut(s) 2200
Bsp1286I GDGCHC 9 cut(s) 45, 297, 1216, 1632, 2204, 2826, 3388, 4025, 4311
Bsp13I TCCGGA 1 cut(s) 4274
Bsp1720I GCTNAGC 1 cut(s) 2489
Bsp68I TCGCGA 1 cut(s) 4432
BspCNI CTCAG 5 cut(s) 373, 385, 1633, 3454, 4906
BspEI TCCGGA 1 cut(s) 4274
BspFNI CGCG 3 cut(s) 325, 4432, 4512
BspHI TCATGA 1 cut(s) 2349
BspLI GGNNCC 9 cut(s) 63, 380, 519, 642, 1639, 2127, 2201, 2202, 4308
BspMAI CTGCAG 5 cut(s) 442, 669, 711, 1333, 1457
BspMI ACCTGC 1 cut(s) 2569
BspOI GCTAGC 1 cut(s) 2982
BspPI GGATC 7 cut(s) 1085, 2043, 2281, 3138, 3749, 3962, 3963
BspT107I GGYRCC 2 cut(s) 640, 4306
BspTI CTTAAG 2 cut(s) 452, 617
BspTNI GGTCTC 1 cut(s) 1265
BsrDI GCAATG 9 cut(s) 501, 1059, 1611, 2631, 2724, 3091, 3738, 3999, 4618
BsrI ACTGG 6 cut(s) 424, 874, 2081, 3409, 4333, 4393
BssNI GRCGYC 1 cut(s) 1571
BssSI CACGAG 1 cut(s) 3490
BssT1I CCWWGG 3 cut(s) 382, 3683, 4602
Bst2BI CACGAG 1 cut(s) 3490
Bst4CI ACNGT 7 cut(s) 82, 221, 946, 1509, 1971, 3352, 4047
Bst6I CTCTTC 5 cut(s) 201, 504, 1196, 1851, 2647
BstACI GRCGYC 1 cut(s) 1571
BstAFI CTTAAG 2 cut(s) 452, 617
BstAPI GCANNNNNTGC 3 cut(s) 1024, 3383, 4808
BstDSI CCRYGG 1 cut(s) 3787
BstFNI CGCG 3 cut(s) 325, 4432, 4512
BstHHI GCGC 3 cut(s) 327, 2964, 4512
BstMAI GTCTC 7 cut(s) 1265, 1562, 1607, 2122, 3460, 3984, 4552
BstMCI CGRYCG 2 cut(s) 2858, 4516
BstNSI RCATGY 4 cut(s) 371, 1602, 2372, 3125
BstSFI CTRYAG 7 cut(s) 438, 665, 707, 1329, 1453, 3114, 3623
BstSLI GKGCMC 4 cut(s) 2204, 3388, 4025, 4311
BstUI CGCG 3 cut(s) 325, 4432, 4512
BstV2I GAAGAC 5 cut(s) 135, 1902, 3429, 3615, 4407
BstX2I RGATCY 5 cut(s) 1090, 2048, 2273, 2867, 3754
BstXI CCANNNNNNTGG 3 cut(s) 1648, 2185, 4683
BstYI RGATCY 5 cut(s) 1090, 2048, 2273, 2867, 3754
Bsu36I CCTNAGG 2 cut(s) 1620, 4182
BsuI GTATCC 1 cut(s) 4188
BtgI CCRYGG 1 cut(s) 3787
BtsI GCAGTG 1 cut(s) 2971
BtsIMutI CAGTG 3 cut(s) 226, 867, 2971
BtuMI TCGCGA 1 cut(s) 4432
BveI ACCTGC 1 cut(s) 2569
CaiI CAGNNNCTG 2 cut(s) 4298, 4728
CciI TCATGA 1 cut(s) 2349
CfoI GCGC 3 cut(s) 327, 2964, 4512
CseI GACGC 2 cut(s) 1266, 1579
Csp6I GTAC 9 cut(s) 417, 912, 942, 1154, 1933, 3362, 4146, 4689, 4875
CspCI CAANNNNNGTGG 2 cut(s) 4360, 4395
CviQI GTAC 9 cut(s) 417, 912, 942, 1154, 1933, 3362, 4146, 4689, 4875
DraI TTTAAA 1 cut(s) 1287
DraIII CACNNNGTG 1 cut(s) 1024
DrdI GACNNNNNNGTC 1 cut(s) 566
DseDI GACNNNNNNGTC 1 cut(s) 566
EaeI YGGCCR 2 cut(s) 3054, 3345
Eam1104I CTCTTC 5 cut(s) 201, 504, 1196, 1851, 2647
EarI CTCTTC 5 cut(s) 201, 504, 1196, 1851, 2647
Ecl136II GAGCTC 2 cut(s) 43, 295
Eco130I CCWWGG 3 cut(s) 382, 3683, 4602
Eco147I AGGCCT 3 cut(s) 969, 1426, 2760
Eco24I GRGCYC 4 cut(s) 45, 297, 2204, 2826
Eco31I GGTCTC 1 cut(s) 1265
Eco32I GATATC 2 cut(s) 3880, 4101
Eco47I GGWCC 6 cut(s) 379, 1161, 2126, 2828, 4277, 4580
Eco53kI GAGCTC 2 cut(s) 43, 295
Eco57I CTGAAG 7 cut(s) 528, 1181, 1875, 3333, 3348, 4815, 4863
Eco81I CCTNAGG 2 cut(s) 1620, 4182
EcoICRI GAGCTC 2 cut(s) 43, 295
EcoO109I RGGNCCY 4 cut(s) 379, 485, 2200, 2201
EcoRI GAATTC 4 cut(s) 846, 976, 3018, 3287
EcoRV GATATC 2 cut(s) 3880, 4101
EcoT14I CCWWGG 3 cut(s) 382, 3683, 4602
EcoT38I GRGCYC 4 cut(s) 45, 297, 2204, 2826
ErhI CCWWGG 3 cut(s) 382, 3683, 4602
Esp3I CGTCTC 1 cut(s) 1562
FalI AAGNNNNNCTT 6 cut(s) 1358, 1390, 1451, 1483, 3306, 3338
FaqI GGGAC 7 cut(s) 89, 392, 821, 1004, 1423, 2139, 4132
FauI CCCGC 1 cut(s) 330
FauNDI CATATG 2 cut(s) 1647, 4501
FbaI TGATCA 4 cut(s) 193, 1114, 2356, 2551
FblI GTMKAC 3 cut(s) 84, 570, 3622
FriOI GRGCYC 4 cut(s) 45, 297, 2204, 2826
GlaI GCGC 3 cut(s) 326, 2963, 4511
GsaI CCCAGC 3 cut(s) 4687, 4732, 4756
GsuI CTGGAG 2 cut(s) 320, 1574
HapII CCGG 2 cut(s) 1892, 4275
HgaI GACGC 2 cut(s) 1266, 1579
HhaI GCGC 3 cut(s) 327, 2964, 4512
Hin1I GRCGYC 1 cut(s) 1571
Hin6I GCGC 3 cut(s) 325, 2962, 4510
HinP1I GCGC 3 cut(s) 325, 2962, 4510
HincII GTYRAC 4 cut(s) 85, 571, 2058, 2302
HindII GTYRAC 4 cut(s) 85, 571, 2058, 2302
HindIII AAGCTT 5 cut(s) 449, 614, 1764, 1865, 4668
HpaI GTTAAC 1 cut(s) 2302
HpaII CCGG 2 cut(s) 1892, 4275
Hpy166II GTNNAC 9 cut(s) 85, 571, 1360, 1728, 2058, 2302, 3386, 3623, 3823
Hpy8I GTNNAC 9 cut(s) 85, 571, 1360, 1728, 2058, 2302, 3386, 3623, 3823
Hpy99I CGWCG 8 cut(s) 26, 29, 50, 74, 113, 575, 1260, 4020
HpyCH4III ACNGT 7 cut(s) 82, 221, 946, 1509, 1971, 3352, 4047
HpyCH4IV ACGT 4 cut(s) 2298, 2314, 2335, 3849
HpySE526I ACGT 4 cut(s) 2298, 2314, 2335, 3849
Hsp92I GRCGYC 1 cut(s) 1571
HspAI GCGC 3 cut(s) 325, 2962, 4510
Kpn2I TCCGGA 1 cut(s) 4274
Ksp22I TGATCA 4 cut(s) 193, 1114, 2356, 2551
KspAI GTTAAC 1 cut(s) 2302
LmnI GCTCC 5 cut(s) 300, 1637, 1698, 2294, 2384
LweI GCATC 8 cut(s) 1139, 1549, 1816, 1993, 3088, 3595, 4355, 4798
MaeII ACGT 4 cut(s) 2298, 2314, 2335, 3849
MaeIII GTNAC 8 cut(s) 1317, 1493, 1524, 1876, 2603, 2743, 3212, 3479
MfeI CAATTG 3 cut(s) 777, 1520, 1758
MflI RGATCY 5 cut(s) 1090, 2048, 2273, 2867, 3754
MhlI GDGCHC 9 cut(s) 45, 297, 1216, 1632, 2204, 2826, 3388, 4025, 4311
MlsI TGGCCA 2 cut(s) 3056, 3347
MluNI TGGCCA 2 cut(s) 3056, 3347
MlyI GAGTC 3 cut(s) 3901, 4280, 4841
MmeI TCCRAC 5 cut(s) 45, 2449, 2985, 4039, 4364
Mox20I TGGCCA 2 cut(s) 3056, 3347
MroI TCCGGA 1 cut(s) 4274
MroXI GAANNNNTTC 2 cut(s) 846, 1767
MscI TGGCCA 2 cut(s) 3056, 3347
MslI CAYNNNNRTG 5 cut(s) 1106, 1141, 1969, 4164, 4482
Msp20I TGGCCA 2 cut(s) 3056, 3347
MspA1I CMGCKG 3 cut(s) 712, 1984, 4728
MspCI CTTAAG 2 cut(s) 452, 617
MspI CCGG 2 cut(s) 1892, 4275
MunI CAATTG 3 cut(s) 777, 1520, 1758
MvnI CGCG 3 cut(s) 325, 4432, 4512
NdeI CATATG 2 cut(s) 1647, 4501
NheI GCTAGC 1 cut(s) 2978
NlaIV GGNNCC 9 cut(s) 63, 380, 519, 642, 1639, 2127, 2201, 2202, 4308
NmeAIII GCCGAG 1 cut(s) 4568
NmuCI GTSAC 4 cut(s) 1317, 1524, 3212, 3479
NruI TCGCGA 1 cut(s) 4432
NspI RCATGY 4 cut(s) 371, 1602, 2372, 3125
OliI CACNNNNGTG 2 cut(s) 1141, 1969
PaeI GCATGC 1 cut(s) 371
PagI TCATGA 1 cut(s) 2349
PasI CCCWGGG 2 cut(s) 2178, 2204
PceI AGGCCT 3 cut(s) 969, 1426, 2760
PciI ACATGT 3 cut(s) 1598, 2368, 3121
PcsI WCGNNNNNNNCGW 3 cut(s) 45, 129, 4012
PdmI GAANNNNTTC 2 cut(s) 846, 1767
PflFI GACNNNGTC 1 cut(s) 80
PflMI CCANNNNNTGG 1 cut(s) 3052
PfoI TCCNGGA 1 cut(s) 297
Ple19I CGATCG 1 cut(s) 2858
PleI GAGTC 3 cut(s) 3901, 4279, 4841
PpsI GAGTC 3 cut(s) 3901, 4279, 4841
PpuMI RGGWCCY 1 cut(s) 379
PscI ACATGT 3 cut(s) 1598, 2368, 3121
PshBI ATTAAT 1 cut(s) 3230
Psp124BI GAGCTC 2 cut(s) 45, 297
Psp1406I AACGTT 1 cut(s) 2298
Psp5II RGGWCCY 1 cut(s) 379
PspFI CCCAGC 3 cut(s) 4683, 4728, 4752
PspN4I GGNNCC 9 cut(s) 63, 380, 519, 642, 1639, 2127, 2201, 2202, 4308
PspOMI GGGCCC 1 cut(s) 2200
PspPPI RGGWCCY 1 cut(s) 379
PsrI GAACNNNNNNTAC 2 cut(s) 3515, 3547
PstI CTGCAG 5 cut(s) 442, 669, 711, 1333, 1457
PstNI CAGNNNCTG 2 cut(s) 4298, 4728
PsuI RGATCY 5 cut(s) 1090, 2048, 2273, 2867, 3754
PsyI GACNNNGTC 1 cut(s) 80
PvuI CGATCG 1 cut(s) 2858
PvuII CAGCTG 2 cut(s) 712, 4728
RruI TCGCGA 1 cut(s) 4432
RsaI GTAC 9 cut(s) 418, 913, 943, 1155, 1934, 3363, 4147, 4690, 4876
RsaNI GTAC 9 cut(s) 417, 912, 942, 1154, 1933, 3362, 4146, 4689, 4875
RseI CAYNNNNRTG 5 cut(s) 1106, 1141, 1969, 4164, 4482
SacI GAGCTC 2 cut(s) 45, 297
SalI GTCGAC 2 cut(s) 83, 569
ScaI AGTACT 1 cut(s) 1934
SchI GAGTC 3 cut(s) 3901, 4280, 4841
SduI GDGCHC 9 cut(s) 45, 297, 1216, 1632, 2204, 2826, 3388, 4025, 4311
SfaNI GCATC 8 cut(s) 1139, 1549, 1816, 1993, 3088, 3595, 4355, 4798
SfcI CTRYAG 7 cut(s) 438, 665, 707, 1329, 1453, 3114, 3623
SinI GGWCC 6 cut(s) 379, 1161, 2126, 2828, 4277, 4580
SmiMI CAYNNNNRTG 5 cut(s) 1106, 1141, 1969, 4164, 4482
SmlI CTYRAG 3 cut(s) 452, 494, 617
SmoI CTYRAG 3 cut(s) 452, 494, 617
SpeI ACTAGT 1 cut(s) 2060
SphI GCATGC 1 cut(s) 371
SseBI AGGCCT 3 cut(s) 969, 1426, 2760
SspI AATATT 3 cut(s) 2880, 3580, 3715
SstI GAGCTC 2 cut(s) 45, 297
StuI AGGCCT 3 cut(s) 969, 1426, 2760
StyI CCWWGG 3 cut(s) 382, 3683, 4602
TaaI ACNGT 7 cut(s) 82, 221, 946, 1509, 1971, 3352, 4047
TaiI ACGT 4 cut(s) 2301, 2317, 2338, 3852
TatI WGTACW 2 cut(s) 1932, 4145
TauI GCSGC 5 cut(s) 99, 102, 105, 108, 349
TscAI CASTG 3 cut(s) 226, 874, 2971
TseFI GTSAC 4 cut(s) 1317, 1524, 3212, 3479
Tsp45I GTSAC 4 cut(s) 1317, 1524, 3212, 3479
TspGWI ACGGA 6 cut(s) 555, 1761, 2109, 2152, 3884, 4156
TspRI CASTG 3 cut(s) 226, 874, 2971
Tth111I GACNNNGTC 1 cut(s) 80
Van91I CCANNNNNTGG 1 cut(s) 3052
Vha464I CTTAAG 2 cut(s) 452, 617
VneI GTGCAC 1 cut(s) 3384
VpaK11BI GGWCC 6 cut(s) 379, 1161, 2126, 2828, 4277, 4580
VspI ATTAAT 1 cut(s) 3230
XapI RAATTY 9 cut(s) 338, 578, 767, 846, 976, 2770, 3018, 3287, 4010
XceI RCATGY 4 cut(s) 371, 1602, 2372, 3125
XcmI CCANNNNNNNNNTGG 1 cut(s) 4166
XmiI GTMKAC 3 cut(s) 84, 570, 3622
XmnI GAANNNNTTC 2 cut(s) 846, 1767
ZrmI AGTACT 1 cut(s) 1934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.