AT4G35140

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006022
Physical Location & Seq
Reverse (-)
16723683 .. 16726636
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35140.1

Sequence Viewer

Length: 1491 bp
ATGAGTGAGAAAGCGAGGAGAGGTCGATCAAACTTTAATGGACTACATCACCCTGTTGTTGATTTTTGGCGACGAGAGGTCGGTGGAATCTCCTCTCGTAATTTCTCCAATCGCTTCTCCGCCTCCGAGAATTTGGTTCTGCGTTTAGAGATATACAAGAAGCTAGAGAAGCACAAAGGGTGTGTAAACACCGTGAGTTTCAATGCCGAAGGAGATGTTCTGATATCTGGTTCCGATGATCGGCGTGTCGTTCTTTGGGATTGGCAACTTGGGAATGTGAAGCTTTCCTTCCATTCAGGACACGCTAATAACGTTTTTCAAGCAAAGTTTATGCCTTTCTCTGATGATCGAACGATAGTAACCTGTGCTGCTGATGGAATGGTTCGGCGTGCAAGTATTCTTGAGGGTGATAAAGTGGAGACGTCTTTCTTGGGATTGCATCAAGGACGAGCTCATAAACTATGTATAGAGCCAGGAAACCCACATATATTTTACACATGTGGTGAAGATGGATTAGTACAACGTTTTGATCTGAGGACTGAAGCCCCTACAGAACTTTTCACTTGCCGATCAGTTGATCCTAGGAGGAGGAACATGGACGCCATCCAGCTAAATGCGATTGCGATCGACCCGAGAAATTCTAACCTCTTTGCTGTTGGGGGAATGGAAGAGTACGCTCGTCTTTATGATATCCGTAGATTTCAGGGTGAGGGTTTAAATGGTTTTACCCGAGCTGCAGATCACTTTTGTCCTCCACATCTTATTGGCAATGAAGATGTTGGAATAACGGGGTTGGCTTTCTCAGAGCAAAGCGAGCTTCTTGTTTCATACAATGATGAGTTCATCTATCTCTTCACACCCGGTATGGGATTAGGGTCTAACCCTATCCCATCCTCCCCGATATCCAAGAGTCCTGTATCAAAATCAGAATCATCTTCTTCCCCAAAAGATGAGAACGAACACTCAGTTTCTTTGGTTTATAAGGGGCACAAGAACTGTGAGACAGTTAAGGGTGTGAACTTTTTTGGACCTCGGTCTGAATATGTGGTTAGTGGGTCAGACTGTGGGAGGATATTTATTTGGAGGAAAAAGGGTGGGGAGCTTATCCGTGTTATGGAAGCAGATAGGCATGTAGTTAACTGTATTGAGCCTCATCCACATATTCCTGTGCTTGCTAGCAGTGGAATTGAAAGTGACATTAAGGTGTGGACGTCAAAGGCAGCTGAGAGAGCTACGTTACCTGAAAACATCGAACTGCGCAAGCGAACGCCTAGGGGATGGATGTATCGTGTCTCTTCACCGCATGAACTATTGGCGCAACTCTTCTCGCTGCAAAACCGAAGCAGTAGTAGTCCAGAGAGAGAAGGAGAATCTTCTTCAGCCACTGGAAGAGAACTTCTTGATCTTATCCTCACCTTCAATGATCAGAGCGACGATGAAAATGCAACTGATGATGATGATGGTAATAGCCATGAAGACTTCTTCTCTTAA

Protein Analysis

496

Amino Acids

55.49

Weight (kDa)

5.9

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 48 - 179 6.4e-09 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 49 - 182 7e-14 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 49 - 177 5.1e-07 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 51 - 182 5.5e-06 G protein beta WD-40 repeat protein
WDR55 PF24796 52 - 129 7.1e-06 WDR55
WD40 PF00400 53 - 87 3.9e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 53 - 171 1.2e-12 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 54 - 222 1.1e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 58 - 129 8.2e-09 THOC3 beta-propeller domain
WD40_Prp19 PF24814 62 - 235 8.4e-11 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 320 - 403 1.6e-11 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AatII GACGTC 2 cut(s) 425, 1214
Acc16I TGCGCA 1 cut(s) 1259
AciI CCGC 2 cut(s) 120, 1301
AclI AACGTT 2 cut(s) 312, 523
AclWI GGATC 1 cut(s) 572
AcsI RAATTY 2 cut(s) 130, 637
AcuI CTGAAG 2 cut(s) 561, 1362
AcyI GRCGYC 3 cut(s) 422, 600, 1211
AfaI GTAC 2 cut(s) 519, 674
AfiI CCNNNNNNNGG 3 cut(s) 240, 866, 1114
AflIII ACRYGT 1 cut(s) 497
AgsI TTSAA 4 cut(s) 202, 320, 1190, 1420
AhdI GACNNNNNGTC 1 cut(s) 77
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 9 cut(s) 163, 283, 452, 610, 734, 817, 1102, 1223, 1232
AluI AGCT 9 cut(s) 163, 283, 452, 610, 734, 817, 1102, 1223, 1232
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 3 cut(s) 413, 995, 1297
AlwI GGATC 1 cut(s) 572
AlwNI CAGNNNCTG 1 cut(s) 1385
Ama87I CYCGRG 2 cut(s) 631, 729
ApeKI GCWGC 4 cut(s) 368, 734, 1220, 1330
ApoI RAATTY 2 cut(s) 130, 637
AspA2I CCTAGG 2 cut(s) 581, 1271
AspLEI GCGC 2 cut(s) 1260, 1318
AspS9I GGNCC 1 cut(s) 1028
AsuC2I CCSGG 1 cut(s) 861
AsuHPI GGTGA 6 cut(s) 41, 419, 515, 719, 1290, 1405
AsuNHI GCTAGC 1 cut(s) 1175
AvaI CYCGRG 2 cut(s) 631, 729
AvaII GGWCC 1 cut(s) 1028
AvrII CCTAGG 2 cut(s) 581, 1271
BaeGI GKGCMC 1 cut(s) 990
BanII GRGCYC 1 cut(s) 454
BbsI GAAGAC 1 cut(s) 1482
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 4 cut(s) 355, 721, 1232, 1317
BccI CCATC 6 cut(s) 368, 503, 611, 898, 1272, 1454
BcgI CGANNNNNNTGC 2 cut(s) 1424, 1458
BciT130I CCWGG 1 cut(s) 474
BclI TGATCA 1 cut(s) 1423
BcnI CCSGG 1 cut(s) 861
BcoDI GTCTC 3 cut(s) 413, 995, 1297
BfaI CTAG 4 cut(s) 164, 582, 1176, 1272
BfmI CTRYAG 2 cut(s) 549, 735
BisI GCNGC 4 cut(s) 369, 735, 1221, 1331
BlnI CCTAGG 2 cut(s) 581, 1271
BlsI GCNGC 4 cut(s) 370, 736, 1222, 1332
Bme1390I CCNGG 2 cut(s) 474, 861
Bme18I GGWCC 1 cut(s) 1028
BmeRI GACNNNNNGTC 1 cut(s) 77
BmeT110I CYCGRG 2 cut(s) 631, 729
BmgT120I GGNCC 1 cut(s) 1028
BmiI GGNNCC 1 cut(s) 232
BmrFI CCNGG 2 cut(s) 474, 861
BmsI GCATC 1 cut(s) 448
BmtI GCTAGC 1 cut(s) 1179
BoxI GACNNNNGTC 1 cut(s) 1033
BpiI GAAGAC 1 cut(s) 1482
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 861
BsaBI GATNNNNATC 1 cut(s) 623
BsaHI GRCGYC 3 cut(s) 422, 600, 1211
BsaJI CCNNGG 3 cut(s) 581, 1031, 1271
Bsc4I CCNNNNNNNGG 3 cut(s) 240, 866, 1114
Bse1I ACTGG 1 cut(s) 1390
Bse3DI GCAATG 1 cut(s) 775
Bse8I GATNNNNATC 1 cut(s) 623
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 3 cut(s) 581, 1031, 1271
BseGI GGATG 5 cut(s) 603, 890, 1153, 1283, 1287
BseJI GATNNNNATC 1 cut(s) 623
BseLI CCNNNNNNNGG 3 cut(s) 240, 866, 1114
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 4 cut(s) 524, 816, 978, 1215
BseNI ACTGG 1 cut(s) 1390
BseRI GAGGAG 3 cut(s) 31, 82, 601
BseSI GKGCMC 1 cut(s) 990
BseXI GCAGC 4 cut(s) 355, 721, 1232, 1317
Bsh1285I CGRYCG 1 cut(s) 627
BsiEI CGRYCG 1 cut(s) 627
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 2 cut(s) 631, 729
BsiSI CCGG 1 cut(s) 861
BslI CCNNNNNNNGG 3 cut(s) 240, 866, 1114
BsmAI GTCTC 3 cut(s) 413, 995, 1297
BsmBI CGTCTC 1 cut(s) 413
BsoBI CYCGRG 2 cut(s) 631, 729
Bsp1286I GDGCHC 2 cut(s) 454, 990
BspACI CCGC 2 cut(s) 120, 1301
BspCNI CTCAG 4 cut(s) 525, 815, 977, 1216
BspLI GGNNCC 1 cut(s) 232
BspMAI CTGCAG 1 cut(s) 739
BspOI GCTAGC 1 cut(s) 1179
BspPI GGATC 1 cut(s) 572
BsrDI GCAATG 1 cut(s) 775
BsrI ACTGG 1 cut(s) 1390
BssECI CCNNGG 3 cut(s) 581, 1031, 1271
BssNI GRCGYC 3 cut(s) 422, 600, 1211
BssT1I CCWWGG 2 cut(s) 581, 1271
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 5 cut(s) 193, 998, 1006, 1064, 1142
Bst6I CTCTTC 5 cut(s) 663, 857, 1300, 1328, 1384
BstACI GRCGYC 3 cut(s) 422, 600, 1211
BstC8I GCNNGC 5 cut(s) 390, 815, 1173, 1177, 1262
BstDEI CTNAG 4 cut(s) 533, 802, 964, 1224
BstF5I GGATG 5 cut(s) 603, 890, 1153, 1283, 1287
BstHHI GCGC 2 cut(s) 1260, 1318
BstMAI GTCTC 3 cut(s) 413, 995, 1297
BstMCI CGRYCG 1 cut(s) 627
BstMWI GCNNNNNNNGC 3 cut(s) 169, 814, 1229
BstNI CCWGG 1 cut(s) 474
BstNSI RCATGY 2 cut(s) 501, 1133
BstPAI GACNNNNGTC 1 cut(s) 1033
BstSCI CCNGG 2 cut(s) 472, 859
BstSFI CTRYAG 2 cut(s) 549, 735
BstSLI GKGCMC 1 cut(s) 990
BstV1I GCAGC 4 cut(s) 355, 721, 1232, 1317
BstV2I GAAGAC 1 cut(s) 1482
BtsCI GGATG 5 cut(s) 603, 890, 1153, 1283, 1287
BtsI GCAGTG 1 cut(s) 1186
BtsIMutI CAGTG 2 cut(s) 1186, 1383
Cac8I GCNNGC 5 cut(s) 390, 815, 1173, 1177, 1262
CaiI CAGNNNCTG 1 cut(s) 1385
CfoI GCGC 2 cut(s) 1260, 1318
Cfr13I GGNCC 1 cut(s) 1028
CseI GACGC 1 cut(s) 608
Csp6I GTAC 2 cut(s) 518, 673
CviAII CATG 5 cut(s) 498, 595, 1130, 1304, 1472
CviQI GTAC 2 cut(s) 518, 673
DdeI CTNAG 4 cut(s) 533, 802, 964, 1224
DraI TTTAAA 1 cut(s) 717
DriI GACNNNNNGTC 1 cut(s) 77
Eam1104I CTCTTC 5 cut(s) 663, 857, 1300, 1328, 1384
Eam1105I GACNNNNNGTC 1 cut(s) 77
EarI CTCTTC 5 cut(s) 663, 857, 1300, 1328, 1384
EciI GGCGGA 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 452
Eco130I CCWWGG 2 cut(s) 581, 1271
Eco24I GRGCYC 1 cut(s) 454
Eco32I GATATC 3 cut(s) 225, 691, 903
Eco47I GGWCC 1 cut(s) 1028
Eco53kI GAGCTC 1 cut(s) 452
Eco57I CTGAAG 2 cut(s) 561, 1362
Eco88I CYCGRG 2 cut(s) 631, 729
EcoICRI GAGCTC 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 472
EcoRV GATATC 3 cut(s) 225, 691, 903
EcoT14I CCWWGG 2 cut(s) 581, 1271
EcoT38I GRGCYC 1 cut(s) 454
ErhI CCWWGG 2 cut(s) 581, 1271
Esp3I CGTCTC 1 cut(s) 413
FaeI CATG 5 cut(s) 501, 598, 1133, 1307, 1475
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FatI CATG 5 cut(s) 497, 594, 1129, 1303, 1471
FbaI TGATCA 1 cut(s) 1423
Fnu4HI GCNGC 4 cut(s) 369, 735, 1221, 1331
FokI GGATG 5 cut(s) 590, 877, 1140, 1290, 1294
FriOI GRGCYC 1 cut(s) 454
Fsp4HI GCNGC 4 cut(s) 369, 735, 1221, 1331
FspBI CTAG 4 cut(s) 164, 582, 1176, 1272
FspI TGCGCA 1 cut(s) 1259
GlaI GCGC 2 cut(s) 1259, 1317
GluI GCNGC 4 cut(s) 369, 735, 1221, 1331
HapII CCGG 1 cut(s) 861
HgaI GACGC 1 cut(s) 608
HhaI GCGC 2 cut(s) 1260, 1318
Hin1I GRCGYC 3 cut(s) 422, 600, 1211
Hin1II CATG 5 cut(s) 501, 598, 1133, 1307, 1475
Hin6I GCGC 2 cut(s) 1258, 1316
HinP1I GCGC 2 cut(s) 1258, 1316
HincII GTYRAC 1 cut(s) 1138
HindII GTYRAC 1 cut(s) 1138
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 4 cut(s) 87, 910, 929, 1370
HpaI GTTAAC 1 cut(s) 1138
HpaII CCGG 1 cut(s) 861
HphI GGTGA 6 cut(s) 41, 419, 515, 719, 1290, 1405
Hpy166II GTNNAC 4 cut(s) 187, 1018, 1138, 1209
Hpy188III TCNNGA 4 cut(s) 297, 401, 1355, 1400
Hpy8I GTNNAC 4 cut(s) 187, 1018, 1138, 1209
Hpy99I CGWCG 2 cut(s) 75, 1436
HpyAV CCTTC 4 cut(s) 203, 298, 1358, 1426
HpyCH4III ACNGT 5 cut(s) 193, 998, 1006, 1064, 1142
HpyCH4IV ACGT 5 cut(s) 312, 422, 523, 1211, 1235
HpyCH4V TGCA 5 cut(s) 392, 439, 737, 1333, 1445
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 814, 1229
HpyF3I CTNAG 4 cut(s) 533, 802, 964, 1224
HpySE526I ACGT 5 cut(s) 312, 422, 523, 1211, 1235
Hsp92I GRCGYC 3 cut(s) 422, 600, 1211
Hsp92II CATG 5 cut(s) 501, 598, 1133, 1307, 1475
HspAI GCGC 2 cut(s) 1258, 1316
Ksp22I TGATCA 1 cut(s) 1423
KspAI GTTAAC 1 cut(s) 1138
LmnI GCTCC 1 cut(s) 1099
Lsp1109I GCAGC 4 cut(s) 355, 721, 1232, 1317
LweI GCATC 1 cut(s) 448
MaeI CTAG 4 cut(s) 164, 582, 1176, 1272
MaeII ACGT 5 cut(s) 312, 422, 523, 1211, 1235
MaeIII GTNAC 3 cut(s) 358, 1193, 1236
MhlI GDGCHC 2 cut(s) 454, 990
MluCI AATT 4 cut(s) 100, 130, 637, 1185
MlyI GAGTC 1 cut(s) 919
MmeI TCCRAC 1 cut(s) 760
MseI TTAA 6 cut(s) 36, 716, 1008, 1137, 1200, 1489
MslI CAYNNNNRTG 1 cut(s) 1202
MspA1I CMGCKG 1 cut(s) 1223
MspI CCGG 1 cut(s) 861
MspR9I CCNGG 2 cut(s) 474, 861
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 3 cut(s) 169, 814, 1229
NciI CCSGG 1 cut(s) 861
NheI GCTAGC 1 cut(s) 1175
NlaIII CATG 5 cut(s) 501, 598, 1133, 1307, 1475
NlaIV GGNNCC 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 1193
NsbI TGCGCA 1 cut(s) 1259
NspI RCATGY 2 cut(s) 501, 1133
PciI ACATGT 1 cut(s) 497
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PfeI GAWTC 3 cut(s) 87, 929, 1370
PkrI GCNGC 4 cut(s) 370, 736, 1222, 1332
Ple19I CGATCG 1 cut(s) 627
PleI GAGTC 1 cut(s) 918
PpsI GAGTC 1 cut(s) 918
PscI ACATGT 1 cut(s) 497
PshAI GACNNNNGTC 1 cut(s) 1033
PsiI TTATAA 1 cut(s) 981
Psp124BI GAGCTC 1 cut(s) 454
Psp1406I AACGTT 2 cut(s) 312, 523
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 1 cut(s) 1028
PstI CTGCAG 1 cut(s) 739
PstNI CAGNNNCTG 1 cut(s) 1385
PvuI CGATCG 1 cut(s) 627
PvuII CAGCTG 1 cut(s) 1223
RsaI GTAC 2 cut(s) 519, 674
RsaNI GTAC 2 cut(s) 518, 673
RseI CAYNNNNRTG 1 cut(s) 1202
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 6 cut(s) 36, 716, 1008, 1137, 1200, 1489
SatI GCNGC 4 cut(s) 369, 735, 1221, 1331
Sau96I GGNCC 1 cut(s) 1028
SchI GAGTC 1 cut(s) 919
ScrFI CCNGG 2 cut(s) 474, 861
SduI GDGCHC 2 cut(s) 454, 990
SfaNI GCATC 1 cut(s) 448
SfcI CTRYAG 2 cut(s) 549, 735
SinI GGWCC 1 cut(s) 1028
SmiMI CAYNNNNRTG 1 cut(s) 1202
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 4 cut(s) 100, 130, 637, 1185
SsiI CCGC 2 cut(s) 120, 1301
SspMI CTAG 4 cut(s) 164, 582, 1176, 1272
SstI GAGCTC 1 cut(s) 454
StyD4I CCNGG 2 cut(s) 472, 859
StyI CCWWGG 2 cut(s) 581, 1271
TaaI ACNGT 5 cut(s) 193, 998, 1006, 1064, 1142
TaiI ACGT 5 cut(s) 315, 425, 526, 1214, 1238
TaqI TCGA 4 cut(s) 25, 349, 627, 1251
TaqII GACCGA 1 cut(s) 1023
TasI AATT 4 cut(s) 100, 130, 637, 1185
TatI WGTACW 1 cut(s) 517
TfiI GAWTC 3 cut(s) 87, 929, 1370
Tru1I TTAA 6 cut(s) 36, 716, 1008, 1137, 1200, 1489
Tru9I TTAA 6 cut(s) 36, 716, 1008, 1137, 1200, 1489
TscAI CASTG 2 cut(s) 1186, 1390
TseFI GTSAC 1 cut(s) 1193
TseI GCWGC 4 cut(s) 368, 734, 1220, 1330
Tsp45I GTSAC 1 cut(s) 1193
TspDTI ATGAA 6 cut(s) 786, 816, 832, 1320, 1452, 1488
TspGWI ACGGA 2 cut(s) 683, 1097
TspRI CASTG 2 cut(s) 1186, 1390
VpaK11BI GGWCC 1 cut(s) 1028
XapI RAATTY 2 cut(s) 130, 637
XceI RCATGY 2 cut(s) 501, 1133
XmaJI CCTAGG 2 cut(s) 581, 1271
XspI CTAG 4 cut(s) 164, 582, 1176, 1272
ZraI GACGTC 2 cut(s) 423, 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.