Rh6BG235600

DDB1- and CUL4-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0006022
Physical Location & Seq
Reverse (-)
44206333 .. 44210697
4365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG235600.1

Sequence Viewer

Length: 1392 bp
ATGATGAATAAGCGATTGAAAACGAGCAGCGACAAGGCTGTCGTCAATGTTTGGCAGCGACAGCTGGGCGAGCTCTCGGCCAGAAACTTTGCTCACCGTCTCGGTGGCTCTGAGGATCTCGTGCTTCGACTTGATGTCCACAGGAAGCTGGACAAGCATAGAGGATGTGTGAACACCGTAAGCTTCAATGCAGATGGCGATATTTTGCTTTCGGGTTCTGATGACAGGCGGCTCATACTATGGAACTGGGAAACGGGGAAAGTTAAGTTGTCATTCCATTCCGGTCATAATAGCAATGTTTTCCAAGCAAAGTTCATGCCTTATAGTGATGATCGAAGCATTGTCACTTGTGCTGCTGATGGGCAGGTGAGACATACTCAGATTTTAGAACGAGGACAAGTGGAATGTTCATTGTTGGCCAGACACCGTGGACGCGCTCATAAGTTAGCTATTGAGCCTGGGAGCCCTCATATTTTCTATACCTGTGGTGAAGATGGACTGGTGCAACATTTTGATCTCAGAACTGGGGCTCCCACAAAACTTTTGACATGCCAAACACTTGATGGTAGAAGGAGTTACTTGCCAGTTATCAGTCTAAATGCAATTGCAATTGACCCAAGAAATCCAAATCTCTTTGCAGTCGCAGGTTCTGATGAGTATACCCGGCTTTTTGATATCCGCAAGTATAACTGGGATGGATCATCTGATTTTGGTCAACCCACGAACTACTTCTGCCCACCACATTTGAATGGTAATGACCAAGTTGGAATTACAGGCTTGGCCTTTTCAGAACAAAGTGAGCTTCTTGTATCATACAATGACGAGTTCATCTATCTTTTTACACGAGAAATGGGATTAGGACCTAATCCTCCCATTTCCATGAGTAGTGATACAAGTGAAATGAGTGATGATCACCAGTCTGAAGAATCTCCATCATCCATGGATACTGATGAAAAAACTATTCCTCAAGTTTACAAGGGTCACTTGAACTCTGAGACAGTGAAAGGTGTGAACTTCTTTGGGCCGAAATGCGAGTATGTTGTGAGTGGGTCTGACTGTGGGCGGATATTCATATGGAAGAAAAAGGGAGGTGAGTTGATTCGTGTCATGGAAGGAGATAAGGATGTAGTAAACTGTATCAAGTCTCATCCTCACACCATGGTACTTGCAAGCAGTGGAATTGAAAATGATATTAAAATGTGGACTCCAAAGGCTCTAGAGAGAGCTACTCTGCCTCAGAACATTGAACAGGTATGCTTTGTTACCCTGAGCTGTTTATTTATGTGTATAATCTCAGTAAGGTTTATAAATTTGCTCATCCAACTTTCCCATGGGGCTTATCTTGCACATCCTAGTTGTGAGCTTAGATGGTGTTGTGGCCCTGAAGATTAG

Protein Analysis

463

Amino Acids

51.89

Weight (kDa)

6.19

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 5 - 130 2.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 43 - 135 1.5e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 45 - 183 1.3e-15 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 46 - 171 9.8e-13 WDR3 first beta-propeller domain
WD40 PF00400 48 - 82 8.8e-07 WD domain, G-beta repeat
WDR55 PF24796 49 - 174 1.7e-07 WDR55
WD40_CDC20-Fz PF24807 51 - 226 6.2e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 53 - 124 3.8e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 57 - 265 6.6e-12 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 315 - 401 1.1e-13 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1307
AarI CACCTGC 1 cut(s) 355
AasI GACNNNNNNGTC 1 cut(s) 38
Acc36I ACCTGC 2 cut(s) 355, 635
AccI GTMKAC 1 cut(s) 659
AccII CGCG 1 cut(s) 435
AciI CCGC 3 cut(s) 229, 679, 1063
AclWI GGATC 2 cut(s) 123, 706
AcoI YGGCCR 2 cut(s) 78, 417
AcsI RAATTY 1 cut(s) 1309
AcuI CTGAAG 1 cut(s) 942
AfaI GTAC 1 cut(s) 1164
AgsI TTSAA 6 cut(s) 19, 187, 748, 988, 1184, 1247
AhdI GACNNNNNGTC 1 cut(s) 134
AjnI CCWGG 1 cut(s) 457
AloI GAACNNNNNNTCC 2 cut(s) 514, 546
AluBI AGCT 9 cut(s) 64, 73, 148, 183, 449, 802, 1226, 1272, 1363
AluI AGCT 9 cut(s) 64, 73, 148, 183, 449, 802, 1226, 1272, 1363
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 4 cut(s) 104, 364, 989, 1149
AlwI GGATC 2 cut(s) 123, 706
AlwNI CAGNNNCTG 1 cut(s) 650
AoxI GGCC 5 cut(s) 78, 417, 780, 1022, 1378
ApeKI GCWGC 3 cut(s) 27, 55, 353
ApoI RAATTY 1 cut(s) 1309
Asp700I GAANNNNTTC 1 cut(s) 728
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 3 cut(s) 860, 1022, 1379
AsuC2I CCSGG 1 cut(s) 664
AsuHPI GGTGA 5 cut(s) 86, 379, 500, 905, 1103
AvaII GGWCC 1 cut(s) 860
BalI TGGCCA 1 cut(s) 419
BanII GRGCYC 3 cut(s) 75, 467, 532
BauI CACGAG 2 cut(s) 119, 843
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 3 cut(s) 39, 67, 340
BccI CCATC 7 cut(s) 188, 353, 488, 557, 689, 940, 1362
BciT130I CCWGG 1 cut(s) 459
BciVI GTATCC 1 cut(s) 937
BclI TGATCA 1 cut(s) 910
BcnI CCSGG 1 cut(s) 664
BcoDI GTCTC 4 cut(s) 104, 364, 989, 1149
BfaI CTAG 2 cut(s) 1217, 1353
BfuAI ACCTGC 2 cut(s) 355, 635
BfuI GTATCC 1 cut(s) 937
BisI GCNGC 4 cut(s) 28, 56, 230, 354
BlsI GCNGC 4 cut(s) 29, 57, 231, 355
Bme1390I CCNGG 2 cut(s) 459, 664
Bme18I GGWCC 1 cut(s) 860
BmeRI GACNNNNNGTC 1 cut(s) 134
BmgT120I GGNCC 3 cut(s) 860, 1022, 1379
BmiI GGNNCC 2 cut(s) 464, 531
BmrFI CCNGG 2 cut(s) 459, 664
BmrI ACTGGG 3 cut(s) 256, 534, 700
BmuI ACTGGG 3 cut(s) 256, 534, 700
BplI GAGNNNNNCTC 4 cut(s) 361, 393, 1213, 1245
Bpu10I CCTNAGC 1 cut(s) 1268
BpuEI CTTGAG 1 cut(s) 951
BpuMI CCSGG 1 cut(s) 664
BsaJI CCNNGG 5 cut(s) 427, 458, 939, 1158, 1330
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bse1I ACTGG 6 cut(s) 251, 504, 529, 584, 695, 916
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 5 cut(s) 427, 458, 939, 1158, 1330
BseGI GGATG 7 cut(s) 170, 700, 935, 1129, 1147, 1317, 1348
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 7 cut(s) 102, 392, 532, 984, 1250, 1259, 1308
BseNI ACTGG 6 cut(s) 251, 504, 529, 584, 695, 916
BseXI GCAGC 3 cut(s) 39, 67, 340
BseYI CCCAGC 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 5 cut(s) 80, 419, 782, 1024, 1380
BsiHKAI GWGCWC 1 cut(s) 75
BsiSI CCGG 2 cut(s) 282, 664
BsmAI GTCTC 4 cut(s) 104, 364, 989, 1149
BsmBI CGTCTC 1 cut(s) 104
BsnI GGCC 5 cut(s) 80, 419, 782, 1024, 1380
Bsp1286I GDGCHC 3 cut(s) 75, 467, 532
Bsp143I GATC 5 cut(s) 115, 331, 514, 698, 910
Bsp19I CCATGG 3 cut(s) 939, 1158, 1330
BspACI CCGC 3 cut(s) 229, 679, 1063
BspANI GGCC 5 cut(s) 80, 419, 782, 1024, 1380
BspCNI CTCAG 7 cut(s) 103, 391, 531, 985, 1249, 1260, 1307
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 2 cut(s) 464, 531
BspMI ACCTGC 2 cut(s) 355, 635
BspPI GGATC 2 cut(s) 123, 706
BsrDI GCAATG 1 cut(s) 301
BsrI ACTGG 6 cut(s) 251, 504, 529, 584, 695, 916
BssECI CCNNGG 5 cut(s) 427, 458, 939, 1158, 1330
BssMI GATC 5 cut(s) 115, 331, 514, 698, 910
BssNAI GTATAC 1 cut(s) 660
BssSI CACGAG 2 cut(s) 119, 843
BssT1I CCWWGG 3 cut(s) 939, 1158, 1330
Bst1107I GTATAC 1 cut(s) 660
Bst2BI CACGAG 2 cut(s) 119, 843
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 6 cut(s) 98, 178, 428, 1000, 1058, 1136
BstC8I GCNNGC 2 cut(s) 71, 1171
BstDEI CTNAG 8 cut(s) 111, 378, 518, 993, 1236, 1268, 1294, 1364
BstDSI CCRYGG 4 cut(s) 427, 939, 1158, 1330
BstF5I GGATG 7 cut(s) 170, 700, 935, 1129, 1147, 1317, 1348
BstFNI CGCG 1 cut(s) 435
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 5 cut(s) 118, 334, 517, 701, 913
BstMAI GTCTC 4 cut(s) 104, 364, 989, 1149
BstMBI GATC 5 cut(s) 115, 331, 514, 698, 910
BstMWI GCNNNNNNNGC 4 cut(s) 61, 70, 154, 1343
BstNI CCWGG 1 cut(s) 459
BstNSI RCATGY 1 cut(s) 552
BstSCI CCNGG 2 cut(s) 457, 662
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 3 cut(s) 39, 67, 340
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BstZ17I GTATAC 1 cut(s) 660
BsuI GTATCC 1 cut(s) 937
BsuRI GGCC 5 cut(s) 80, 419, 782, 1024, 1380
BtgI CCRYGG 4 cut(s) 427, 939, 1158, 1330
BtsCI GGATG 7 cut(s) 170, 700, 935, 1129, 1147, 1317, 1348
BtsI GCAGTG 1 cut(s) 1180
BtsIMutI CAGTG 2 cut(s) 1005, 1180
BveI ACCTGC 2 cut(s) 355, 635
Cac8I GCNNGC 2 cut(s) 71, 1171
CaiI CAGNNNCTG 1 cut(s) 650
CfoI GCGC 1 cut(s) 437
Cfr13I GGNCC 3 cut(s) 860, 1022, 1379
CseI GACGC 1 cut(s) 441
Csp6I GTAC 1 cut(s) 1163
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 7 cut(s) 316, 549, 880, 940, 1108, 1159, 1331
CviQI GTAC 1 cut(s) 1163
DdeI CTNAG 8 cut(s) 111, 378, 518, 993, 1236, 1268, 1294, 1364
DpnI GATC 5 cut(s) 117, 333, 516, 700, 912
DpnII GATC 5 cut(s) 115, 331, 514, 698, 910
DrdI GACNNNNNNGTC 1 cut(s) 38
DriI GACNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 2 cut(s) 78, 417
Eam1105I GACNNNNNGTC 1 cut(s) 134
EciI GGCGGA 1 cut(s) 1078
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 3 cut(s) 939, 1158, 1330
Eco24I GRGCYC 3 cut(s) 75, 467, 532
Eco32I GATATC 1 cut(s) 676
Eco47I GGWCC 1 cut(s) 860
Eco53kI GAGCTC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 942
EcoICRI GAGCTC 1 cut(s) 73
EcoO109I RGGNCCY 1 cut(s) 860
EcoRII CCWGG 1 cut(s) 457
EcoRV GATATC 1 cut(s) 676
EcoT14I CCWWGG 3 cut(s) 939, 1158, 1330
EcoT38I GRGCYC 3 cut(s) 75, 467, 532
ErhI CCWWGG 3 cut(s) 939, 1158, 1330
Esp3I CGTCTC 1 cut(s) 104
FaeI CATG 7 cut(s) 319, 552, 883, 943, 1111, 1162, 1334
FalI AAGNNNNNCTT 2 cut(s) 968, 1000
FatI CATG 7 cut(s) 315, 548, 879, 939, 1107, 1158, 1330
FauNDI CATATG 1 cut(s) 1073
FbaI TGATCA 1 cut(s) 910
FblI GTMKAC 1 cut(s) 659
Fnu4HI GCNGC 4 cut(s) 28, 56, 230, 354
FokI GGATG 7 cut(s) 177, 707, 922, 1134, 1136, 1304, 1335
FriOI GRGCYC 3 cut(s) 75, 467, 532
Fsp4HI GCNGC 4 cut(s) 28, 56, 230, 354
FspBI CTAG 2 cut(s) 1217, 1353
GlaI GCGC 1 cut(s) 436
GluI GCNGC 4 cut(s) 28, 56, 230, 354
GsaI CCCAGC 1 cut(s) 68
HaeIII GGCC 5 cut(s) 80, 419, 782, 1024, 1380
HapII CCGG 2 cut(s) 282, 664
HgaI GACGC 1 cut(s) 441
HhaI GCGC 1 cut(s) 437
Hin1II CATG 7 cut(s) 319, 552, 883, 943, 1111, 1162, 1334
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HincII GTYRAC 1 cut(s) 716
HindII GTYRAC 1 cut(s) 716
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 3 cut(s) 926, 1099, 1204
HpaII CCGG 2 cut(s) 282, 664
HphI GGTGA 5 cut(s) 86, 379, 500, 905, 1103
Hpy166II GTNNAC 9 cut(s) 139, 172, 431, 660, 716, 973, 1012, 1132, 1203
Hpy188III TCNNGA 1 cut(s) 1217
Hpy8I GTNNAC 9 cut(s) 139, 172, 431, 660, 716, 973, 1012, 1132, 1203
HpyAV CCTTC 2 cut(s) 564, 1106
HpyCH4III ACNGT 6 cut(s) 98, 178, 428, 1000, 1058, 1136
HpyCH4V TGCA 7 cut(s) 191, 505, 602, 608, 638, 1169, 1346
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 70, 154, 1343
HpyF3I CTNAG 8 cut(s) 111, 378, 518, 993, 1236, 1268, 1294, 1364
Hsp92II CATG 7 cut(s) 319, 552, 883, 943, 1111, 1162, 1334
HspAI GCGC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 910
Kzo9I GATC 5 cut(s) 115, 331, 514, 698, 910
LmnI GCTCC 2 cut(s) 462, 535
Lsp1109I GCAGC 3 cut(s) 39, 67, 340
MaeI CTAG 2 cut(s) 1217, 1353
MaeIII GTNAC 4 cut(s) 343, 575, 980, 1261
MalI GATC 5 cut(s) 117, 333, 516, 700, 912
MboI GATC 5 cut(s) 115, 331, 514, 698, 910
MboII GAAGA 3 cut(s) 503, 935, 1090
MfeI CAATTG 2 cut(s) 603, 609
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 3 cut(s) 75, 467, 532
MlsI TGGCCA 1 cut(s) 419
MluCI AATT 5 cut(s) 603, 609, 768, 1179, 1309
MluNI TGGCCA 1 cut(s) 419
MlyI GAGTC 1 cut(s) 1198
MmeI TCCRAC 2 cut(s) 745, 1345
MnlI CCTC 9 cut(s) 106, 155, 386, 477, 879, 975, 1082, 1161, 1245
Mox20I TGGCCA 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 728
MscI TGGCCA 1 cut(s) 419
MseI TTAA 2 cut(s) 264, 1194
MslI CAYNNNNRTG 2 cut(s) 747, 878
Msp20I TGGCCA 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 64
MspI CCGG 2 cut(s) 282, 664
MspR9I CCNGG 2 cut(s) 459, 664
MunI CAATTG 2 cut(s) 603, 609
MvaI CCWGG 1 cut(s) 459
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 4 cut(s) 61, 70, 154, 1343
NciI CCSGG 1 cut(s) 664
NcoI CCATGG 3 cut(s) 939, 1158, 1330
NdeI CATATG 1 cut(s) 1073
NdeII GATC 5 cut(s) 115, 331, 514, 698, 910
NlaIII CATG 7 cut(s) 319, 552, 883, 943, 1111, 1162, 1334
NlaIV GGNNCC 2 cut(s) 464, 531
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 2 cut(s) 343, 980
NspI RCATGY 1 cut(s) 552
PaqCI CACCTGC 1 cut(s) 355
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 2 cut(s) 926, 1099
PkrI GCNGC 4 cut(s) 29, 57, 231, 355
PleI GAGTC 1 cut(s) 1198
PpsI GAGTC 1 cut(s) 1198
PpuMI RGGWCCY 1 cut(s) 860
PsiI TTATAA 1 cut(s) 1307
Psp124BI GAGCTC 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 860
Psp6I CCWGG 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 2 cut(s) 464, 531
PspPI GGNCC 3 cut(s) 860, 1022, 1379
PspPPI RGGWCCY 1 cut(s) 860
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 64
RsaI GTAC 1 cut(s) 1164
RsaNI GTAC 1 cut(s) 1163
RseI CAYNNNNRTG 2 cut(s) 747, 878
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 2 cut(s) 264, 1194
SatI GCNGC 4 cut(s) 28, 56, 230, 354
Sau3AI GATC 5 cut(s) 115, 331, 514, 698, 910
Sau96I GGNCC 3 cut(s) 860, 1022, 1379
SchI GAGTC 1 cut(s) 1198
ScrFI CCNGG 2 cut(s) 459, 664
SduI GDGCHC 3 cut(s) 75, 467, 532
SinI GGWCC 1 cut(s) 860
SmiMI CAYNNNNRTG 2 cut(s) 747, 878
SmlI CTYRAG 1 cut(s) 966
SmoI CTYRAG 1 cut(s) 966
Sse9I AATT 5 cut(s) 603, 609, 768, 1179, 1309
SsiI CCGC 3 cut(s) 229, 679, 1063
SspMI CTAG 2 cut(s) 1217, 1353
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 2 cut(s) 457, 662
StyI CCWWGG 3 cut(s) 939, 1158, 1330
TaaI ACNGT 6 cut(s) 98, 178, 428, 1000, 1058, 1136
TaqI TCGA 2 cut(s) 127, 334
TasI AATT 5 cut(s) 603, 609, 768, 1179, 1309
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 2 cut(s) 926, 1099
Tru1I TTAA 2 cut(s) 264, 1194
Tru9I TTAA 2 cut(s) 264, 1194
TscAI CASTG 2 cut(s) 1005, 1180
TseFI GTSAC 2 cut(s) 343, 980
TseI GCWGC 3 cut(s) 27, 55, 353
Tsp45I GTSAC 2 cut(s) 343, 980
TspDTI ATGAA 6 cut(s) 20, 304, 399, 817, 966, 1060
TspRI CASTG 2 cut(s) 1005, 1180
VpaK11BI GGWCC 1 cut(s) 860
XapI RAATTY 1 cut(s) 1309
XbaI TCTAGA 1 cut(s) 1216
XceI RCATGY 1 cut(s) 552
XcmI CCANNNNNNNNNTGG 2 cut(s) 560, 1328
XmiI GTMKAC 1 cut(s) 659
XmnI GAANNNNTTC 1 cut(s) 728
XspI CTAG 2 cut(s) 1217, 1353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.