FvH4_2g15750

DDB1- and CUL4-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006022
Physical Location & Seq
Reverse (-)
13805658 .. 13808978
3321 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15750.t3

Sequence Viewer

Length: 1542 bp
ATGATGAATAAGAGATTGAGAACGAGCACCGATAAACCCGTCGTCAATGTTTGGCAGCGACAGCTCGGCGAGCTCTCTGCCAGAAACTTCGCCCACCGCCTCGGTGGCTCTGAGGATCTGGTGCTTCGGCTTGATATCCACGGGAAGCTGAACAAGCATCAAGGGTGTGTGAACACCGTAAGCTTCAATGCAGATGGTGATACTTTGGTTTCAGGTTCTGATGACACGCGGCTTATACTATGGAACTGGGAAACCGGGAAAGTTAACTTGTCATTCGATTCCGGTCATAATAACAATGTTTTCCAAGCAAAGTTCATGCCTTATAGTAACGATCGAAGCATTGTCACTTGTGCTGCTGATGGACAGGTGAGACATATTCAGATTTCTGAACATGGGCAAGTGGAGAGTTCATTGTTGGCCTCACATACTAGACGCGCTCATAAGTTAGCTATTGAGCCTGGAAGCCCTCATATTTTCTATACCTGTGGTGAAGATGGACTGGTGCTACATTTTGATCTCAGAACTGGGGCTCCCACAAAACTTTTGACATGCCGAGGGCATGATGTTAGAGTGAGGTACAAGCCAGTTGTCAAGCTAAATGCAATTGCAATTGACCCAAGAAATCCAAATCTCTTTGCAGTCGCAGGTTCTGATGAGTATACCCGACTTTTTGATATCCGCAAATATAACTGGGATGGATCATCCGATTTTGGTCAGCCTACAAACTACTTCTGCCCACCACATTTGATTGGTAATGACCAAGTTGGAATTACAGGCTTGGCCTTTTCAGAACAAAGTGAGCTTCTTGTATCATATAATGACGAGTTCATCTATCTTTTTACACGAGAAATGGGGTTAGGACCTAATCCTCCCATTTCTATGAGTAGTGATGGAAGTGAAATGAGTGATGATCCCAAGTCTAAAGCATCTCCATCATCCATGGATACTGATGAAAAAACCGTTCCTCAAGTTTACAAGGGTCACATGAACTCTGAGACTGTGAAAGGTGTGAACTTCTTTGGGCCGAATTGCGAGTATGTTGTGAGTGGGTCTGACTGTGGGAGGATATTCATATGGAAAAAAAAGGGCGGGGAATTGATTCGTGTCATGGAAGGAGATAAGCATGTGGTAAACTGTATCGAGTCTCATCCTCACACCATGGTACTTGCAAGCAGTGGAATTGAGCATGACATTAAGATGTGGACTCCCAAGGCTCTAGAGAGAGCTCCCCTGCCTCAGAACATTGAACAGGGCGCGATACCTGGCCGATTAATATGGTACACTATTGGTGATGAGGATGAGGATGATGAATATTCTTATGACGACTATATGTTTGATGACGAGAACGATGAAGATGACGAAGACGAGGATGATGATGATGATAGTGATGATCAGGACGAGGATGACGATGATAGTGATGATCAGGACGAGGATGACGATGATAGTGATGGTGGAAATGTTTTGAATGAAAGTGGCAGAAACTGTGAAGATGATGATGCTAACAATCGTGATAGTTTCCTTGATGGTTATGATCTACACTAA

Protein Analysis

514

Amino Acids

57.5

Weight (kDa)

4.47

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 15 - 189 2.8e-14 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 65 - 160 4.1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 72 - 125 3.5e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 74 - 166 2.6e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 75 - 214 3.1e-16 WDR5 beta-propeller domain
EIF3I PF24805 78 - 218 1.4e-06 EIF3I
WD40 PF00400 79 - 112 2.3e-06 WD domain, G-beta repeat
WDR55 PF24796 79 - 154 1e-05 WDR55
WD40_CDC20-Fz PF24807 80 - 256 6.2e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 83 - 155 2.3e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 85 - 260 3e-12 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 141 - 225 1.9e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 345 - 431 2.4e-13 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 635
AccI GTMKAC 1 cut(s) 659
AccII CGCG 3 cut(s) 229, 435, 1256
AciI CCGC 4 cut(s) 97, 229, 679, 1089
AclWI GGATC 3 cut(s) 123, 706, 905
AcoI YGGCCR 1 cut(s) 1264
AfaI GTAC 3 cut(s) 578, 1164, 1280
AgsI TTSAA 3 cut(s) 187, 1247, 1465
AjnI CCWGG 2 cut(s) 457, 1261
AloI GAACNNNNNNTCC 2 cut(s) 514, 546
AluBI AGCT 8 cut(s) 64, 73, 148, 183, 449, 595, 802, 1226
AluI AGCT 8 cut(s) 64, 73, 148, 183, 449, 595, 802, 1226
Alw21I GWGCWC 3 cut(s) 29, 75, 1228
Alw26I GTCTC 3 cut(s) 364, 989, 1149
AlwI GGATC 3 cut(s) 123, 706, 905
AlwNI CAGNNNCTG 3 cut(s) 218, 650, 1482
AoxI GGCC 4 cut(s) 417, 780, 1022, 1264
ApeKI GCWGC 2 cut(s) 55, 353
ArsI GACNNNNNNTTYG 2 cut(s) 1316, 1348
AseI ATTAAT 1 cut(s) 1271
Asp700I GAANNNNTTC 1 cut(s) 1098
AspLEI GCGC 2 cut(s) 437, 1256
AspS9I GGNCC 2 cut(s) 860, 1022
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 4 cut(s) 209, 379, 500, 1301
AvaII GGWCC 1 cut(s) 860
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BanII GRGCYC 3 cut(s) 75, 532, 1228
BauI CACGAG 1 cut(s) 843
BbsI GAAGAC 1 cut(s) 1368
Bbv12I GWGCWC 3 cut(s) 29, 75, 1228
BbvI GCAGC 2 cut(s) 67, 340
BccI CCATC 8 cut(s) 188, 353, 488, 689, 884, 940, 1442, 1517
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BciT130I CCWGG 2 cut(s) 459, 1263
BciVI GTATCC 1 cut(s) 937
BclI TGATCA 2 cut(s) 1390, 1420
BcnI CCSGG 1 cut(s) 256
BcoDI GTCTC 3 cut(s) 364, 989, 1149
BfaI CTAG 2 cut(s) 429, 1217
BfuAI ACCTGC 1 cut(s) 635
BfuI GTATCC 1 cut(s) 937
BglI GCCNNNNNGGC 1 cut(s) 105
BisI GCNGC 3 cut(s) 56, 230, 354
BlsI GCNGC 3 cut(s) 57, 231, 355
Bme1390I CCNGG 3 cut(s) 256, 459, 1263
Bme18I GGWCC 1 cut(s) 860
BmgT120I GGNCC 2 cut(s) 860, 1022
BmiI GGNNCC 1 cut(s) 531
BmrFI CCNGG 3 cut(s) 256, 459, 1263
BmrI ACTGGG 3 cut(s) 256, 534, 700
BmsI GCATC 3 cut(s) 166, 935, 1486
BmuI ACTGGG 3 cut(s) 256, 534, 700
BpiI GAAGAC 1 cut(s) 1368
BpuEI CTTGAG 1 cut(s) 951
BpuMI CCSGG 1 cut(s) 256
BsaJI CCNNGG 6 cut(s) 100, 139, 553, 939, 1158, 1209
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bse1I ACTGG 5 cut(s) 251, 504, 529, 584, 695
BseBI CCWGG 2 cut(s) 459, 1263
BseDI CCNNGG 6 cut(s) 100, 139, 553, 939, 1158, 1209
BseGI GGATG 9 cut(s) 700, 701, 935, 1147, 1303, 1309, 1375, 1408, 1438
BseMII CTCAG 4 cut(s) 102, 532, 984, 1250
BseNI ACTGG 5 cut(s) 251, 504, 529, 584, 695
BseXI GCAGC 2 cut(s) 67, 340
Bsh1236I CGCG 3 cut(s) 229, 435, 1256
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 4 cut(s) 419, 782, 1024, 1266
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 3 cut(s) 29, 75, 1228
BsiSI CCGG 2 cut(s) 255, 282
BsmAI GTCTC 3 cut(s) 364, 989, 1149
BsnI GGCC 4 cut(s) 419, 782, 1024, 1266
Bsp1286I GDGCHC 4 cut(s) 29, 75, 532, 1228
Bsp143I GATC 8 cut(s) 115, 331, 514, 698, 910, 1390, 1420, 1531
Bsp19I CCATGG 2 cut(s) 939, 1158
BspACI CCGC 4 cut(s) 97, 229, 679, 1089
BspANI GGCC 4 cut(s) 419, 782, 1024, 1266
BspCNI CTCAG 4 cut(s) 103, 531, 985, 1249
BspFNI CGCG 3 cut(s) 229, 435, 1256
BspLI GGNNCC 1 cut(s) 531
BspMI ACCTGC 1 cut(s) 635
BspPI GGATC 3 cut(s) 123, 706, 905
BsrI ACTGG 5 cut(s) 251, 504, 529, 584, 695
BssECI CCNNGG 6 cut(s) 100, 139, 553, 939, 1158, 1209
BssMI GATC 8 cut(s) 115, 331, 514, 698, 910, 1390, 1420, 1531
BssNAI GTATAC 1 cut(s) 660
BssSI CACGAG 1 cut(s) 843
BssT1I CCWWGG 3 cut(s) 939, 1158, 1209
Bst1107I GTATAC 1 cut(s) 660
Bst2BI CACGAG 1 cut(s) 843
Bst2UI CCWGG 2 cut(s) 459, 1263
Bst4CI ACNGT 6 cut(s) 178, 961, 1000, 1058, 1136, 1484
BstC8I GCNNGC 2 cut(s) 71, 1171
BstDEI CTNAG 4 cut(s) 111, 518, 993, 1236
BstDSI CCRYGG 3 cut(s) 139, 939, 1158
BstF5I GGATG 9 cut(s) 700, 701, 935, 1147, 1303, 1309, 1375, 1408, 1438
BstFNI CGCG 3 cut(s) 229, 435, 1256
BstHHI GCGC 2 cut(s) 437, 1256
BstKTI GATC 8 cut(s) 118, 334, 517, 701, 913, 1393, 1423, 1534
BstMAI GTCTC 3 cut(s) 364, 989, 1149
BstMBI GATC 8 cut(s) 115, 331, 514, 698, 910, 1390, 1420, 1531
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 4 cut(s) 61, 70, 105, 154
BstNI CCWGG 2 cut(s) 459, 1263
BstNSI RCATGY 2 cut(s) 552, 1127
BstSCI CCNGG 3 cut(s) 254, 457, 1261
BstUI CGCG 3 cut(s) 229, 435, 1256
BstV1I GCAGC 2 cut(s) 67, 340
BstV2I GAAGAC 1 cut(s) 1368
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BstZ17I GTATAC 1 cut(s) 660
BsuI GTATCC 1 cut(s) 937
BsuRI GGCC 4 cut(s) 419, 782, 1024, 1266
BtgI CCRYGG 3 cut(s) 139, 939, 1158
BtsCI GGATG 9 cut(s) 700, 701, 935, 1147, 1303, 1309, 1375, 1408, 1438
BtsI GCAGTG 1 cut(s) 1180
BtsIMutI CAGTG 1 cut(s) 1180
BveI ACCTGC 1 cut(s) 635
Cac8I GCNNGC 2 cut(s) 71, 1171
CaiI CAGNNNCTG 3 cut(s) 218, 650, 1482
CfoI GCGC 2 cut(s) 437, 1256
Cfr13I GGNCC 2 cut(s) 860, 1022
CseI GACGC 1 cut(s) 441
Csp6I GTAC 3 cut(s) 577, 1163, 1279
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviQI GTAC 3 cut(s) 577, 1163, 1279
DdeI CTNAG 4 cut(s) 111, 518, 993, 1236
DpnI GATC 8 cut(s) 117, 333, 516, 700, 912, 1392, 1422, 1533
DpnII GATC 8 cut(s) 115, 331, 514, 698, 910, 1390, 1420, 1531
EaeI YGGCCR 1 cut(s) 1264
Ecl136II GAGCTC 2 cut(s) 73, 1226
Eco130I CCWWGG 3 cut(s) 939, 1158, 1209
Eco24I GRGCYC 3 cut(s) 75, 532, 1228
Eco32I GATATC 2 cut(s) 136, 676
Eco47I GGWCC 1 cut(s) 860
Eco53kI GAGCTC 2 cut(s) 73, 1226
EcoICRI GAGCTC 2 cut(s) 73, 1226
EcoO109I RGGNCCY 1 cut(s) 860
EcoRII CCWGG 2 cut(s) 457, 1261
EcoRV GATATC 2 cut(s) 136, 676
EcoT14I CCWWGG 3 cut(s) 939, 1158, 1209
EcoT38I GRGCYC 3 cut(s) 75, 532, 1228
ErhI CCWWGG 3 cut(s) 939, 1158, 1209
FauI CCCGC 1 cut(s) 1082
FauNDI CATATG 1 cut(s) 1073
FbaI TGATCA 2 cut(s) 1390, 1420
FblI GTMKAC 1 cut(s) 659
Fnu4HI GCNGC 3 cut(s) 56, 230, 354
FokI GGATG 9 cut(s) 688, 707, 922, 1134, 1310, 1316, 1382, 1415, 1445
FriOI GRGCYC 3 cut(s) 75, 532, 1228
Fsp4HI GCNGC 3 cut(s) 56, 230, 354
FspBI CTAG 2 cut(s) 429, 1217
GlaI GCGC 2 cut(s) 436, 1255
GluI GCNGC 3 cut(s) 56, 230, 354
HaeIII GGCC 4 cut(s) 419, 782, 1024, 1266
HapII CCGG 2 cut(s) 255, 282
HgaI GACGC 1 cut(s) 441
HhaI GCGC 2 cut(s) 437, 1256
Hin6I GCGC 2 cut(s) 435, 1254
HinP1I GCGC 2 cut(s) 435, 1254
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 4 cut(s) 278, 1099, 1142, 1204
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 2 cut(s) 255, 282
HphI GGTGA 4 cut(s) 209, 379, 500, 1301
Hpy166II GTNNAC 8 cut(s) 172, 265, 660, 973, 1012, 1132, 1203, 1281
Hpy188III TCNNGA 4 cut(s) 1217, 1394, 1424, 1508
Hpy8I GTNNAC 8 cut(s) 172, 265, 660, 973, 1012, 1132, 1203, 1281
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 1 cut(s) 1106
HpyCH4III ACNGT 6 cut(s) 178, 961, 1000, 1058, 1136, 1484
HpyCH4V TGCA 5 cut(s) 191, 602, 608, 638, 1169
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 70, 105, 154
HpyF3I CTNAG 4 cut(s) 111, 518, 993, 1236
HspAI GCGC 2 cut(s) 435, 1254
Ksp22I TGATCA 2 cut(s) 1390, 1420
KspAI GTTAAC 1 cut(s) 265
Kzo9I GATC 8 cut(s) 115, 331, 514, 698, 910, 1390, 1420, 1531
LmnI GCTCC 2 cut(s) 535, 1231
Lsp1109I GCAGC 2 cut(s) 67, 340
LweI GCATC 3 cut(s) 166, 935, 1486
MaeI CTAG 2 cut(s) 429, 1217
MaeIII GTNAC 3 cut(s) 326, 343, 980
MalI GATC 8 cut(s) 117, 333, 516, 700, 912, 1392, 1422, 1533
MboI GATC 8 cut(s) 115, 331, 514, 698, 910, 1390, 1420, 1531
MboII GAAGA 4 cut(s) 503, 1364, 1373, 1499
MfeI CAATTG 2 cut(s) 603, 609
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 4 cut(s) 29, 75, 532, 1228
MluCI AATT 6 cut(s) 603, 609, 768, 1027, 1094, 1179
MlyI GAGTC 2 cut(s) 1151, 1198
MmeI TCCRAC 1 cut(s) 745
MroXI GAANNNNTTC 1 cut(s) 1098
MseI TTAA 3 cut(s) 264, 1194, 1271
MslI CAYNNNNRTG 2 cut(s) 878, 1196
MspI CCGG 2 cut(s) 255, 282
MspR9I CCNGG 3 cut(s) 256, 459, 1263
MunI CAATTG 2 cut(s) 603, 609
MvaI CCWGG 2 cut(s) 459, 1263
MvnI CGCG 3 cut(s) 229, 435, 1256
MwoI GCNNNNNNNGC 4 cut(s) 61, 70, 105, 154
NciI CCSGG 1 cut(s) 256
NcoI CCATGG 2 cut(s) 939, 1158
NdeI CATATG 1 cut(s) 1073
NdeII GATC 8 cut(s) 115, 331, 514, 698, 910, 1390, 1420, 1531
NlaIV GGNNCC 1 cut(s) 531
NmeAIII GCCGAG 2 cut(s) 45, 578
NmuCI GTSAC 2 cut(s) 343, 980
NspI RCATGY 2 cut(s) 552, 1127
PcsI WCGNNNNNNNCGW 2 cut(s) 1404, 1434
PdmI GAANNNNTTC 1 cut(s) 1098
PfeI GAWTC 2 cut(s) 278, 1099
PkrI GCNGC 3 cut(s) 57, 231, 355
Ple19I CGATCG 1 cut(s) 334
PleI GAGTC 2 cut(s) 1150, 1198
PpsI GAGTC 2 cut(s) 1150, 1198
PpuMI RGGWCCY 1 cut(s) 860
PshBI ATTAAT 1 cut(s) 1271
Psp124BI GAGCTC 2 cut(s) 75, 1228
Psp5II RGGWCCY 1 cut(s) 860
Psp6I CCWGG 2 cut(s) 457, 1261
PspGI CCWGG 2 cut(s) 457, 1261
PspN4I GGNNCC 1 cut(s) 531
PspPI GGNCC 2 cut(s) 860, 1022
PspPPI RGGWCCY 1 cut(s) 860
PstNI CAGNNNCTG 3 cut(s) 218, 650, 1482
PsuI RGATCY 1 cut(s) 115
PvuI CGATCG 1 cut(s) 334
RsaI GTAC 3 cut(s) 578, 1164, 1280
RsaNI GTAC 3 cut(s) 577, 1163, 1279
RseI CAYNNNNRTG 2 cut(s) 878, 1196
SacI GAGCTC 2 cut(s) 75, 1228
SaqAI TTAA 3 cut(s) 264, 1194, 1271
SatI GCNGC 3 cut(s) 56, 230, 354
Sau3AI GATC 8 cut(s) 115, 331, 514, 698, 910, 1390, 1420, 1531
Sau96I GGNCC 2 cut(s) 860, 1022
SchI GAGTC 2 cut(s) 1151, 1198
ScrFI CCNGG 3 cut(s) 256, 459, 1263
SduI GDGCHC 4 cut(s) 29, 75, 532, 1228
SfaNI GCATC 3 cut(s) 166, 935, 1486
SinI GGWCC 1 cut(s) 860
SmiMI CAYNNNNRTG 2 cut(s) 878, 1196
SmlI CTYRAG 1 cut(s) 966
SmoI CTYRAG 1 cut(s) 966
Sse9I AATT 6 cut(s) 603, 609, 768, 1027, 1094, 1179
SsiI CCGC 4 cut(s) 97, 229, 679, 1089
SspI AATATT 1 cut(s) 1313
SspMI CTAG 2 cut(s) 429, 1217
SstI GAGCTC 2 cut(s) 75, 1228
StyD4I CCNGG 3 cut(s) 254, 457, 1261
StyI CCWWGG 3 cut(s) 939, 1158, 1209
TaaI ACNGT 6 cut(s) 178, 961, 1000, 1058, 1136, 1484
TaqI TCGA 3 cut(s) 276, 334, 1140
TasI AATT 6 cut(s) 603, 609, 768, 1027, 1094, 1179
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 2 cut(s) 278, 1099
Tru1I TTAA 3 cut(s) 264, 1194, 1271
Tru9I TTAA 3 cut(s) 264, 1194, 1271
TscAI CASTG 1 cut(s) 1180
TseFI GTSAC 2 cut(s) 343, 980
TseI GCWGC 2 cut(s) 55, 353
Tsp45I GTSAC 2 cut(s) 343, 980
TspRI CASTG 1 cut(s) 1180
VpaK11BI GGWCC 1 cut(s) 860
VspI ATTAAT 1 cut(s) 1271
XbaI TCTAGA 1 cut(s) 1216
XceI RCATGY 2 cut(s) 552, 1127
XcmI CCANNNNNNNNNTGG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 659
XmnI GAANNNNTTC 1 cut(s) 1098
XspI CTAG 2 cut(s) 429, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.