MD05G1126500.v1.1

DDB1- and CUL4-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006022
Physical Location & Seq
Reverse (-)
24965043 .. 24969032
3990 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1126500.v1.1.491

Sequence Viewer

Length: 1662 bp
ATGAACACGAGGCATCGAACAAGCATCGACAAGGCTGTCGTCAATGTTTGGCAGCGGGAGGTGGGCCAGCTCTCCGCCAGAAATTTCAAGCACCGCCTCGCCGGCTCTGAGGATCTCGTGCTCCGACTTGAAATCTTTCGGAAGCTAGAGAAGCATAAAGGATGTGTGAACACTGTAAGCTTCAATGCCGATGGTGATATTCTGGTTTCGGGCTCTGATGACAGGCGGGTTATCCTATGGAATTGGGAAACTGGGAAAGCTCAGCTTTCTTTCCATTCCGGTCATAATAATAATATATTCCAAGCAAAGATCATGCCTTATACCGATGATCGCACAATTGTTACTTGTGCTGCTGATGGGCAGGTGAGACGTTCTCAAATTCTAGAGCGGGGACAAGTGAATACTTCATTGTTGGGCAGACATATGGGCCGTGCTCATAAGTTGGCCGTTGAGCCTGGGAGTCCTCATATTTTTTACACCTGTGGTGAAGATGGATTGGTGCAACATTTTGATCTGAGGACTGATGCTGCCACAGAACTTTTCACGTGCAAACCTCTTGATAATAGGAGTTACATGCCAGCCATCCATCTAAATGCAATTGCAATTGACCCAAGAAACCCAAATTTCTTTGCAGTGGCAGGTGCTGATGAATATGCACGACTTTTTGATATCCGTAAGTATAACTGGGATGGGTCATCTGATGTTGGCCAACCTGCAGACTTCTATTGCCCACCACACTTGATTGGTGATGTACAAGTGGGAATTACAGGATTGGCCTTCTCAGAACAGAGCGAACTTCTTGTCTCATATAATGATGAGTTCATATATCTTTTTACTCGAGATATGGGATTGGGACCTAATCCAATTCCATCCTCTCCAGTTTCTATGGGAAGTGATGCAAGTGAAACGAGTGGTGATCATCAGTGTGAAAAATCTCCATCAACTATGGATGTTGATAAAAGATTTGGTCCTCAGGATTACAAGGGTCATAGGAACTGTGAGACAGTAAAAGGTGTGAACTTCTTTGGGCCTAATTGTGAGTATGTTGTGAGCGGGTCTGATTGTGGGCGGATATTCATATGGAAGAAAAAGGGTGGGGAGCTGATTCGTGTTATGGAAGCGGATAAGCATGTGGTAAATTGCATCGAGTCTCATCCTCACACCACAGTTCTTGCAAGCAGTGGAATTGAAAATGACATAAAGATGTGGACTCCCAAGGCCAATGAGAGAGCTATTCTGCCTCAGAACATCGAGCAGGTCGTGATACCTGGCCGAATAATGTGGTATGCTAGTGAGGAGGAGGAGGATGAGGATGATGATGAATATTTTTCAGAAGACGATATTTTTGATGATGATGATTATGATGACAATGACGATGACGATGACTGCGACGAAGATGACGATGATGATTTAGATGACTATGCAGTTTTCTTGATGAAGAAACCGAAGGCTAGGGGCTGGATGTACCGCTTATCGTCCCCTCAGAATTTGATGATGCAACTATTTTCACTGCAAAGGCAGAGTGAAAGCCCGGATCGCACGGGGGAGAACTCATCTTCCGGTCGGGACCTTCTAGAGCTAATACTGACATTTGAGGCAAACAATGATGATTCTTCAGGCGATGAAGGAGACGCCGCTAGCCTGGATGATTTGTTTAGTTGA

Protein Analysis

554

Amino Acids

62.25

Weight (kDa)

4.6

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 38 - 94 1.2e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 43 - 171 3.9e-07 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 43 - 173 7.4e-09 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 44 - 182 4.1e-14 WDR5 beta-propeller domain
WD40_Gbeta PF25391 44 - 177 5.2e-07 G protein beta WD-40 repeat protein
WD40 PF00400 47 - 81 7.1e-08 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 47 - 165 2.8e-12 WDR3 first beta-propeller domain
WDR55 PF24796 47 - 123 1.6e-06 WDR55
WD40_CDC20-Fz PF24807 49 - 225 3.4e-07 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 52 - 123 3e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 54 - 228 2.7e-11 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 324 - 403 6.2e-12 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 352, 629
AasI GACNNNNNNGTC 1 cut(s) 35
Acc36I ACCTGC 4 cut(s) 352, 629, 721, 1246
AccBSI CCGCTC 2 cut(s) 388, 1053
AclWI GGATC 2 cut(s) 120, 1542
AcoI YGGCCR 3 cut(s) 444, 706, 1270
AcsI RAATTY 4 cut(s) 82, 378, 622, 1486
AcuI CTGAAG 1 cut(s) 1599
AcvI CACGTG 1 cut(s) 546
AcyI GRCGYC 1 cut(s) 1632
AfaI GTAC 2 cut(s) 753, 1466
AgsI TTSAA 4 cut(s) 88, 131, 184, 1190
AjnI CCWGG 3 cut(s) 454, 1267, 1641
AloI GAACNNNNNNTCC 2 cut(s) 1541, 1573
AluBI AGCT 8 cut(s) 70, 145, 180, 260, 265, 1102, 1232, 1579
AluI AGCT 8 cut(s) 70, 145, 180, 260, 265, 1102, 1232, 1579
Alw21I GWGCWC 2 cut(s) 123, 436
Alw26I GTCTC 5 cut(s) 361, 808, 995, 1155, 1623
AlwI GGATC 2 cut(s) 120, 1542
AlwNI CAGNNNCTG 1 cut(s) 644
Ama87I CYCGRG 1 cut(s) 837
AoxI GGCC 8 cut(s) 64, 427, 444, 706, 774, 1028, 1218, 1270
ApeKI GCWGC 3 cut(s) 52, 350, 527
ApoI RAATTY 4 cut(s) 82, 378, 622, 1486
ArsI GACNNNNNNTTYG 4 cut(s) 786, 818, 1328, 1360
Asp700I GAANNNNTTC 1 cut(s) 135
AspS9I GGNCC 6 cut(s) 64, 427, 854, 968, 1028, 1567
AsuC2I CCSGG 1 cut(s) 1532
AsuHPI GGTGA 5 cut(s) 206, 376, 497, 758, 926
AsuNHI GCTAGC 1 cut(s) 1637
AvaI CYCGRG 1 cut(s) 837
AvaII GGWCC 3 cut(s) 854, 968, 1567
AxyI CCTNAGG 1 cut(s) 972
BalI TGGCCA 1 cut(s) 708
BanII GRGCYC 1 cut(s) 215
BauI CACGAG 2 cut(s) 7, 116
BbrPI CACGTG 1 cut(s) 546
BbsI GAAGAC 1 cut(s) 1341
Bbv12I GWGCWC 2 cut(s) 123, 436
BbvI GCAGC 3 cut(s) 64, 337, 514
BccI CCATC 8 cut(s) 185, 350, 485, 590, 594, 683, 877, 946
BceAI ACGGC 2 cut(s) 414, 431
BciT130I CCWGG 3 cut(s) 456, 1269, 1643
BclI TGATCA 1 cut(s) 916
BcnI CCSGG 1 cut(s) 1532
BcoDI GTCTC 5 cut(s) 361, 808, 995, 1155, 1623
BfaI CTAG 6 cut(s) 146, 383, 1290, 1452, 1574, 1638
BfmI CTRYAG 1 cut(s) 714
BfuAI ACCTGC 4 cut(s) 352, 629, 721, 1246
BglI GCCNNNNNGGC 1 cut(s) 102
BisI GCNGC 4 cut(s) 53, 351, 528, 1635
BlpI GCTNAGC 1 cut(s) 261
BlsI GCNGC 4 cut(s) 54, 352, 529, 1636
Bme1390I CCNGG 4 cut(s) 456, 1269, 1532, 1643
Bme18I GGWCC 3 cut(s) 854, 968, 1567
BmeT110I CYCGRG 1 cut(s) 837
BmgT120I GGNCC 6 cut(s) 64, 427, 854, 968, 1028, 1567
BmiI GGNNCC 2 cut(s) 855, 1568
BmrFI CCNGG 4 cut(s) 456, 1269, 1532, 1643
BmrI ACTGGG 2 cut(s) 261, 694
BmsI GCATC 6 cut(s) 22, 33, 514, 886, 1152, 1485
BmtI GCTAGC 1 cut(s) 1641
BmuI ACTGGG 2 cut(s) 261, 694
BpiI GAAGAC 1 cut(s) 1341
BplI GAGNNNNNCTC 2 cut(s) 358, 390
BpmI CTGGAG 1 cut(s) 861
Bpu1102I GCTNAGC 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 1532
BsaAI YACGTR 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 1632
BsaJI CCNNGG 2 cut(s) 455, 1215
BsaWI WCCGGW 2 cut(s) 278, 1559
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 3 cut(s) 256, 689, 878
Bse21I CCTNAGG 1 cut(s) 972
BseBI CCWGG 3 cut(s) 456, 1269, 1643
BseDI CCNNGG 2 cut(s) 455, 1215
BseMII CTCAG 7 cut(s) 99, 275, 506, 795, 986, 1256, 1496
BseNI ACTGG 3 cut(s) 256, 689, 878
BseRI GAGGAG 3 cut(s) 1310, 1313, 1316
BseXI GCAGC 3 cut(s) 64, 337, 514
Bsh1285I CGRYCG 1 cut(s) 1564
BshFI GGCC 8 cut(s) 66, 429, 446, 708, 776, 1030, 1220, 1272
BsiEI CGRYCG 1 cut(s) 1564
BsiHKAI GWGCWC 2 cut(s) 123, 436
BsiHKCI CYCGRG 1 cut(s) 837
BsiSI CCGG 4 cut(s) 102, 279, 1532, 1560
BslFI GGGAC 4 cut(s) 405, 867, 1462, 1580
BsmAI GTCTC 5 cut(s) 361, 808, 995, 1155, 1623
BsmBI CGTCTC 2 cut(s) 361, 1623
BsmFI GGGAC 4 cut(s) 405, 867, 1462, 1580
BsnI GGCC 8 cut(s) 66, 429, 446, 708, 776, 1030, 1220, 1272
BsoBI CYCGRG 1 cut(s) 837
Bsp1286I GDGCHC 3 cut(s) 123, 215, 436
Bsp1407I TGTACA 1 cut(s) 751
Bsp143I GATC 6 cut(s) 112, 309, 328, 511, 916, 1534
Bsp1720I GCTNAGC 1 cut(s) 261
BspANI GGCC 8 cut(s) 66, 429, 446, 708, 776, 1030, 1220, 1272
BspCNI CTCAG 7 cut(s) 100, 274, 507, 794, 985, 1255, 1495
BspLI GGNNCC 2 cut(s) 855, 1568
BspMAI CTGCAG 1 cut(s) 718
BspMI ACCTGC 4 cut(s) 352, 629, 721, 1246
BspOI GCTAGC 1 cut(s) 1641
BspPI GGATC 2 cut(s) 120, 1542
BsrBI CCGCTC 2 cut(s) 388, 1053
BsrFI RCCGGY 1 cut(s) 101
BsrGI TGTACA 1 cut(s) 751
BsrI ACTGG 3 cut(s) 256, 689, 878
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 455, 1215
BssMI GATC 6 cut(s) 112, 309, 328, 511, 916, 1534
BssNI GRCGYC 1 cut(s) 1632
BssSI CACGAG 2 cut(s) 7, 116
BssT1I CCWWGG 1 cut(s) 1215
Bst2BI CACGAG 2 cut(s) 7, 116
Bst2UI CCWGG 3 cut(s) 456, 1269, 1643
Bst4CI ACNGT 4 cut(s) 175, 998, 1006, 1168
BstACI GRCGYC 1 cut(s) 1632
BstAUI TGTACA 1 cut(s) 751
BstBAI YACGTR 1 cut(s) 546
BstC8I GCNNGC 5 cut(s) 68, 103, 579, 1177, 1639
BstDEI CTNAG 7 cut(s) 108, 261, 515, 781, 972, 1242, 1482
BstKTI GATC 6 cut(s) 115, 312, 331, 514, 919, 1537
BstMAI GTCTC 5 cut(s) 361, 808, 995, 1155, 1623
BstMBI GATC 6 cut(s) 112, 309, 328, 511, 916, 1534
BstMCI CGRYCG 1 cut(s) 1564
BstMWI GCNNNNNNNGC 3 cut(s) 102, 151, 1536
BstNI CCWGG 3 cut(s) 456, 1269, 1643
BstNSI RCATGY 2 cut(s) 577, 1133
BstSCI CCNGG 4 cut(s) 454, 1267, 1530, 1641
BstSFI CTRYAG 1 cut(s) 714
BstV1I GCAGC 3 cut(s) 64, 337, 514
BstV2I GAAGAC 1 cut(s) 1341
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
Bsu36I CCTNAGG 1 cut(s) 972
BsuRI GGCC 8 cut(s) 66, 429, 446, 708, 776, 1030, 1220, 1272
BtgZI GCGATG 1 cut(s) 1635
BtsI GCAGTG 3 cut(s) 639, 1186, 1508
BtsIMutI CAGTG 5 cut(s) 171, 639, 929, 1186, 1508
BveI ACCTGC 4 cut(s) 352, 629, 721, 1246
Cac8I GCNNGC 5 cut(s) 68, 103, 579, 1177, 1639
CaiI CAGNNNCTG 1 cut(s) 644
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 6 cut(s) 64, 427, 854, 968, 1028, 1567
CseI GACGC 1 cut(s) 1640
Csp6I GTAC 2 cut(s) 752, 1465
CspCI CAANNNNNGTGG 4 cut(s) 720, 755, 1153, 1188
CviAII CATG 3 cut(s) 313, 574, 1130
CviQI GTAC 2 cut(s) 752, 1465
DdeI CTNAG 7 cut(s) 108, 261, 515, 781, 972, 1242, 1482
DpnI GATC 6 cut(s) 114, 311, 330, 513, 918, 1536
DpnII GATC 6 cut(s) 112, 309, 328, 511, 916, 1534
DrdI GACNNNNNNGTC 1 cut(s) 35
DseDI GACNNNNNNGTC 1 cut(s) 35
EaeI YGGCCR 3 cut(s) 444, 706, 1270
EciI GGCGGA 2 cut(s) 64, 1084
Eco130I CCWWGG 1 cut(s) 1215
Eco24I GRGCYC 1 cut(s) 215
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 3 cut(s) 854, 968, 1567
Eco57I CTGAAG 1 cut(s) 1599
Eco72I CACGTG 1 cut(s) 546
Eco81I CCTNAGG 1 cut(s) 972
Eco88I CYCGRG 1 cut(s) 837
EcoO109I RGGNCCY 2 cut(s) 854, 1567
EcoRII CCWGG 3 cut(s) 454, 1267, 1641
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 1215
EcoT38I GRGCYC 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 1215
Esp3I CGTCTC 2 cut(s) 361, 1623
FaeI CATG 3 cut(s) 316, 577, 1133
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FaqI GGGAC 4 cut(s) 405, 867, 1462, 1580
FatI CATG 3 cut(s) 312, 573, 1129
FauI CCCGC 4 cut(s) 48, 219, 381, 1046
FauNDI CATATG 2 cut(s) 423, 1079
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 4 cut(s) 53, 351, 528, 1635
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 4 cut(s) 53, 351, 528, 1635
FspBI CTAG 6 cut(s) 146, 383, 1290, 1452, 1574, 1638
GluI GCNGC 4 cut(s) 53, 351, 528, 1635
GsuI CTGGAG 1 cut(s) 861
HaeIII GGCC 8 cut(s) 66, 429, 446, 708, 776, 1030, 1220, 1272
HapII CCGG 4 cut(s) 102, 279, 1532, 1560
HgaI GACGC 1 cut(s) 1640
Hin1I GRCGYC 1 cut(s) 1632
Hin1II CATG 3 cut(s) 316, 577, 1133
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 5 cut(s) 460, 1105, 1148, 1210, 1610
HpaII CCGG 4 cut(s) 102, 279, 1532, 1560
HphI GGTGA 5 cut(s) 206, 376, 497, 758, 926
Hpy166II GTNNAC 3 cut(s) 169, 1018, 1209
Hpy188III TCNNGA 8 cut(s) 383, 557, 839, 974, 1261, 1432, 1565, 1574
Hpy8I GTNNAC 3 cut(s) 169, 1018, 1209
Hpy99I CGWCG 1 cut(s) 1394
HpyAV CCTTC 4 cut(s) 787, 1441, 1580, 1619
HpyCH4III ACNGT 4 cut(s) 175, 998, 1006, 1168
HpyCH4IV ACGT 2 cut(s) 370, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 151, 1536
HpyF3I CTNAG 7 cut(s) 108, 261, 515, 781, 972, 1242, 1482
HpySE526I ACGT 2 cut(s) 370, 545
Hsp92I GRCGYC 1 cut(s) 1632
Hsp92II CATG 3 cut(s) 316, 577, 1133
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 916
Kzo9I GATC 6 cut(s) 112, 309, 328, 511, 916, 1534
LmnI GCTCC 2 cut(s) 126, 1099
Lsp1109I GCAGC 3 cut(s) 64, 337, 514
LweI GCATC 6 cut(s) 22, 33, 514, 886, 1152, 1485
MaeI CTAG 6 cut(s) 146, 383, 1290, 1452, 1574, 1638
MaeII ACGT 2 cut(s) 370, 545
MaeIII GTNAC 2 cut(s) 340, 569
MalI GATC 6 cut(s) 114, 311, 330, 513, 918, 1536
MbiI CCGCTC 2 cut(s) 388, 1053
MboI GATC 6 cut(s) 112, 309, 328, 511, 916, 1534
MboII GAAGA 7 cut(s) 500, 1096, 1346, 1406, 1450, 1548, 1605
MfeI CAATTG 3 cut(s) 336, 597, 603
MflI RGATCY 1 cut(s) 112
MhlI GDGCHC 3 cut(s) 123, 215, 436
MlsI TGGCCA 1 cut(s) 708
MluNI TGGCCA 1 cut(s) 708
MlyI GAGTC 3 cut(s) 469, 1157, 1204
MmeI TCCRAC 1 cut(s) 148
Mox20I TGGCCA 1 cut(s) 708
MroNI GCCGGC 1 cut(s) 101
MroXI GAANNNNTTC 1 cut(s) 135
MscI TGGCCA 1 cut(s) 708
MslI CAYNNNNRTG 3 cut(s) 591, 924, 1202
Msp20I TGGCCA 1 cut(s) 708
MspA1I CMGCKG 1 cut(s) 55
MspI CCGG 4 cut(s) 102, 279, 1532, 1560
MspR9I CCNGG 4 cut(s) 456, 1269, 1532, 1643
MunI CAATTG 3 cut(s) 336, 597, 603
MvaI CCWGG 3 cut(s) 456, 1269, 1643
MwoI GCNNNNNNNGC 3 cut(s) 102, 151, 1536
NaeI GCCGGC 1 cut(s) 103
NciI CCSGG 1 cut(s) 1532
NdeI CATATG 2 cut(s) 423, 1079
NdeII GATC 6 cut(s) 112, 309, 328, 511, 916, 1534
NgoMIV GCCGGC 1 cut(s) 101
NheI GCTAGC 1 cut(s) 1637
NlaIII CATG 3 cut(s) 316, 577, 1133
NlaIV GGNNCC 2 cut(s) 855, 1568
NspI RCATGY 2 cut(s) 577, 1133
PaeR7I CTCGAG 1 cut(s) 837
PaqCI CACCTGC 2 cut(s) 352, 629
PcsI WCGNNNNNNNCGW 3 cut(s) 1257, 1386, 1398
PdiI GCCGGC 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 135
PfeI GAWTC 2 cut(s) 1105, 1610
PkrI GCNGC 4 cut(s) 54, 352, 529, 1636
PleI GAGTC 3 cut(s) 468, 1156, 1204
PmaCI CACGTG 1 cut(s) 546
PmlI CACGTG 1 cut(s) 546
PpsI GAGTC 3 cut(s) 468, 1156, 1204
Ppu21I YACGTR 1 cut(s) 546
PpuMI RGGWCCY 2 cut(s) 854, 1567
Psp5II RGGWCCY 2 cut(s) 854, 1567
Psp6I CCWGG 3 cut(s) 454, 1267, 1641
PspCI CACGTG 1 cut(s) 546
PspGI CCWGG 3 cut(s) 454, 1267, 1641
PspN4I GGNNCC 2 cut(s) 855, 1568
PspPI GGNCC 6 cut(s) 64, 427, 854, 968, 1028, 1567
PspPPI RGGWCCY 2 cut(s) 854, 1567
PstI CTGCAG 1 cut(s) 718
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 1 cut(s) 112
RsaI GTAC 2 cut(s) 753, 1466
RsaNI GTAC 2 cut(s) 752, 1465
RseI CAYNNNNRTG 3 cut(s) 591, 924, 1202
SatI GCNGC 4 cut(s) 53, 351, 528, 1635
Sau3AI GATC 6 cut(s) 112, 309, 328, 511, 916, 1534
Sau96I GGNCC 6 cut(s) 64, 427, 854, 968, 1028, 1567
SchI GAGTC 3 cut(s) 469, 1157, 1204
ScrFI CCNGG 4 cut(s) 456, 1269, 1532, 1643
SduI GDGCHC 3 cut(s) 123, 215, 436
SfaNI GCATC 6 cut(s) 22, 33, 514, 886, 1152, 1485
SfcI CTRYAG 1 cut(s) 714
Sfr274I CTCGAG 1 cut(s) 837
SinI GGWCC 3 cut(s) 854, 968, 1567
SlaI CTCGAG 1 cut(s) 837
SmiMI CAYNNNNRTG 3 cut(s) 591, 924, 1202
SmlI CTYRAG 1 cut(s) 837
SmoI CTYRAG 1 cut(s) 837
SspI AATATT 1 cut(s) 1325
SspMI CTAG 6 cut(s) 146, 383, 1290, 1452, 1574, 1638
StyD4I CCNGG 4 cut(s) 454, 1267, 1530, 1641
StyI CCWWGG 1 cut(s) 1215
TaaI ACNGT 4 cut(s) 175, 998, 1006, 1168
TaiI ACGT 2 cut(s) 373, 548
TaqI TCGA 5 cut(s) 16, 27, 838, 1146, 1251
TatI WGTACW 1 cut(s) 751
TauI GCSGC 1 cut(s) 1637
TfiI GAWTC 2 cut(s) 1105, 1610
TscAI CASTG 5 cut(s) 178, 639, 929, 1186, 1515
TseI GCWGC 3 cut(s) 52, 350, 527
TspDTI ATGAA 8 cut(s) 17, 396, 663, 811, 1066, 1335, 1451, 1638
TspGWI ACGGA 1 cut(s) 662
TspRI CASTG 5 cut(s) 178, 639, 929, 1186, 1515
VpaK11BI GGWCC 3 cut(s) 854, 968, 1567
XapI RAATTY 4 cut(s) 82, 378, 622, 1486
XbaI TCTAGA 2 cut(s) 382, 1573
XceI RCATGY 2 cut(s) 577, 1133
XhoI CTCGAG 1 cut(s) 837
XmnI GAANNNNTTC 1 cut(s) 135
XspI CTAG 6 cut(s) 146, 383, 1290, 1452, 1574, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.