Rw0G021130

DDB1- and CUL4-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00971
OG0006022
Physical Location & Seq
Reverse (-)
6638 .. 10234
3597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G021130.1

Sequence Viewer

Length: 1404 bp
ATGATGAATAAGCGATTGAAAGCGAGCAGCGACAAGGCTGTCGTCAATGTTTGGCAGCGACAGCTGGGCGAGCTCTCCGCCAGAAACTTTGCTCACCGTCTCGGTGGCTCTGAGGATCTCGTGCTTCGACTTGATGTCCACAGGAAGCTGGACAAGCATAGAGGATGTGTGAACACTGTAAGCTTCAATGCAGATGGCGATATTTTGCTTTCGGGTTCTGATGACAGGCGGCTCATACTATGGAACTGGGAAACGGGGAAAGTTAAGTTGTCATTCCATTCTGGTCATAATAGCAATGTTTTCCAAGCAAAGTTCATGCCTTATAGTGATGATCGAAGCATTGTCACTTGTGCTGCTGATGGGCAGGTGAGACATACTCAGATTTTAGAACGAGGACAAGTGGAATGTTCATTGTTGGCCAGACACCGTGGACGCGCTCATAAGTTAGCTATTGAGCTGGGAGCCCTCATATTTTCTATACCTGTGGTGAAGATGGACTGCAATGTGACTGGGCTTTTTATTTTTGATCTCAGAACTGGGGCTCCCACAAAACTTTTGACATGCCAAACACTTGATGGTAGAAGGAGTTACTTGCCAGTTATCAGTCTAAATGCAATTGCAATTGACCCAAGAAATCCAAATCTCTTTGCATATAACTGGGATGGATCATCTGATTTTGGTCAACCCACGAACTACTTCTGCCCACCACATTTGAATGGTAATGACCAAGTTGGAATTACAGGCTTGGCCTTTTCAGAACAAAGTGAGCTTCTTGTATCATACAATGACGAGTTCATCTATCTTTTTACACGAGAAATGGGATTAGGACCTAATCCTCCCATTTCCATGAGTAGTGATACAAGTGAAATGAGTGATGATCACCAGTCTGAAGAATCTCCATCATCCATGGATACTGATGAAAAAACTGTTCCTCAAGTTTACAAGGGTCACTTGAACTCTGAGACAGTGAAAGGTGTGAACTTCTTTGGGCCGAAATGCGAGTATGTTGAGTGGAAAAAGGGAGGTGAGTTGATTCGTGTCATGGAAGGAGATAAGGATGTAGTGAACTGTATCAAGTCTCATCCTCACACCATGGTACTTGCAAGCAGTGGAATTGAAAATGATATTAAGATGTGGACTCCAAAGGCTCTAGAGAGAGCTACTCTGCCTCAGAACATTGAACAGCAGAAACCCAAGGCTAAGGGCTGGATGTACCGCTTCTCGCCTCAGGAATTGATGCTGCAACTATTTTCACTGCAACGGCGGAGGACAAGCCCAGAGCACAGTGGGGAAAACTCATCGGCAGATCTAGAGCTAATACTGACATTAGATGCCAACAATGATGATTCTTCAGGAGGAGAAGAAGACGATGACAACTCCAGCCAGGGAGATTTTGTTTTTTGA

Protein Analysis

467

Amino Acids

52.29

Weight (kDa)

5.57

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 6 - 129 2.9e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5 PF25175 10 - 124 1.1e-11 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 43 - 133 2.4e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 46 - 128 2e-11 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 46 - 144 1.4e-11 WDR3 first beta-propeller domain
WD40 PF00400 48 - 82 8.9e-07 WD domain, G-beta repeat
WDR55 PF24796 49 - 124 3.5e-06 WDR55
Beta-prop_THOC3 PF25174 53 - 124 3.8e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 57 - 145 7.6e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 355
AasI GACNNNNNNGTC 1 cut(s) 38
Acc36I ACCTGC 1 cut(s) 355
AccII CGCG 1 cut(s) 435
AciI CCGC 4 cut(s) 78, 229, 1216, 1264
AclWI GGATC 2 cut(s) 123, 673
AcoI YGGCCR 1 cut(s) 417
AcuI CTGAAG 2 cut(s) 909, 1335
AfaI GTAC 2 cut(s) 1098, 1214
AgsI TTSAA 6 cut(s) 19, 187, 715, 955, 1118, 1181
AhdI GACNNNNNGTC 1 cut(s) 134
AjnI CCWGG 1 cut(s) 1383
AloI GAACNNNNNNTCC 2 cut(s) 526, 558
AluBI AGCT 9 cut(s) 64, 73, 148, 183, 449, 457, 769, 1160, 1315
AluI AGCT 9 cut(s) 64, 73, 148, 183, 449, 457, 769, 1160, 1315
Alw21I GWGCWC 2 cut(s) 75, 1284
Alw26I GTCTC 4 cut(s) 104, 364, 956, 1083
AlwI GGATC 2 cut(s) 123, 673
AoxI GGCC 3 cut(s) 417, 747, 989
ApeKI GCWGC 4 cut(s) 27, 55, 353, 1240
Asp700I GAANNNNTTC 1 cut(s) 695
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 2 cut(s) 827, 989
AsuHPI GGTGA 5 cut(s) 86, 379, 499, 872, 1037
AvaII GGWCC 1 cut(s) 827
AxyI CCTNAGG 1 cut(s) 1227
BalI TGGCCA 1 cut(s) 419
BanII GRGCYC 3 cut(s) 75, 466, 544
BauI CACGAG 2 cut(s) 119, 810
BbsI GAAGAC 1 cut(s) 1371
Bbv12I GWGCWC 2 cut(s) 75, 1284
BbvI GCAGC 4 cut(s) 39, 67, 340, 1227
BccI CCATC 6 cut(s) 188, 353, 487, 569, 656, 907
BceAI ACGGC 1 cut(s) 1277
BciT130I CCWGG 1 cut(s) 1385
BciVI GTATCC 1 cut(s) 904
BclI TGATCA 1 cut(s) 877
BcoDI GTCTC 4 cut(s) 104, 364, 956, 1083
BfaI CTAG 2 cut(s) 1151, 1310
BfuAI ACCTGC 1 cut(s) 355
BfuI GTATCC 1 cut(s) 904
BglII AGATCT 1 cut(s) 1306
BisI GCNGC 5 cut(s) 28, 56, 230, 354, 1241
BlsI GCNGC 5 cut(s) 29, 57, 231, 355, 1242
Bme1390I CCNGG 1 cut(s) 1385
Bme18I GGWCC 1 cut(s) 827
BmeRI GACNNNNNGTC 1 cut(s) 134
BmgT120I GGNCC 2 cut(s) 827, 989
BmiI GGNNCC 2 cut(s) 463, 543
BmrFI CCNGG 1 cut(s) 1385
BmrI ACTGGG 4 cut(s) 256, 519, 546, 667
BmsI GCATC 2 cut(s) 1227, 1321
BmuI ACTGGG 4 cut(s) 256, 519, 546, 667
BpiI GAAGAC 1 cut(s) 1371
BplI GAGNNNNNCTC 4 cut(s) 361, 393, 1147, 1179
BpmI CTGGAG 1 cut(s) 1363
Bpu10I CCTNAGC 1 cut(s) 1200
BpuEI CTTGAG 1 cut(s) 918
BsaJI CCNNGG 5 cut(s) 427, 906, 1092, 1194, 1384
BsaXI ACNNNNNCTCC 3 cut(s) 526, 556, 1380
Bse1I ACTGG 6 cut(s) 251, 514, 541, 596, 662, 883
Bse21I CCTNAGG 1 cut(s) 1227
Bse3DI GCAATG 2 cut(s) 301, 508
BseBI CCWGG 1 cut(s) 1385
BseDI CCNNGG 5 cut(s) 427, 906, 1092, 1194, 1384
BseGI GGATG 6 cut(s) 170, 667, 902, 1063, 1081, 1215
BseMI GCAATG 2 cut(s) 301, 508
BseMII CTCAG 6 cut(s) 102, 392, 544, 951, 1184, 1241
BseNI ACTGG 6 cut(s) 251, 514, 541, 596, 662, 883
BseRI GAGGAG 1 cut(s) 1371
BseXI GCAGC 4 cut(s) 39, 67, 340, 1227
BseYI CCCAGC 2 cut(s) 64, 457
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 3 cut(s) 419, 749, 991
BsiHKAI GWGCWC 2 cut(s) 75, 1284
BsmAI GTCTC 4 cut(s) 104, 364, 956, 1083
BsmBI CGTCTC 1 cut(s) 104
BsnI GGCC 3 cut(s) 419, 749, 991
Bsp1286I GDGCHC 4 cut(s) 75, 466, 544, 1284
Bsp143I GATC 6 cut(s) 115, 331, 526, 665, 877, 1306
Bsp19I CCATGG 2 cut(s) 906, 1092
BspACI CCGC 4 cut(s) 78, 229, 1216, 1264
BspANI GGCC 3 cut(s) 419, 749, 991
BspCNI CTCAG 6 cut(s) 103, 391, 543, 952, 1183, 1240
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 2 cut(s) 463, 543
BspMI ACCTGC 1 cut(s) 355
BspPI GGATC 2 cut(s) 123, 673
BsrDI GCAATG 2 cut(s) 301, 508
BsrI ACTGG 6 cut(s) 251, 514, 541, 596, 662, 883
BssECI CCNNGG 5 cut(s) 427, 906, 1092, 1194, 1384
BssMI GATC 6 cut(s) 115, 331, 526, 665, 877, 1306
BssSI CACGAG 2 cut(s) 119, 810
BssT1I CCWWGG 3 cut(s) 906, 1092, 1194
Bst2BI CACGAG 2 cut(s) 119, 810
Bst2UI CCWGG 1 cut(s) 1385
Bst4CI ACNGT 7 cut(s) 98, 178, 428, 928, 967, 1070, 1286
BstC8I GCNNGC 3 cut(s) 25, 71, 1105
BstDEI CTNAG 7 cut(s) 111, 378, 530, 960, 1170, 1200, 1227
BstDSI CCRYGG 3 cut(s) 427, 906, 1092
BstF5I GGATG 6 cut(s) 170, 667, 902, 1063, 1081, 1215
BstFNI CGCG 1 cut(s) 435
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 6 cut(s) 118, 334, 529, 668, 880, 1309
BstMAI GTCTC 4 cut(s) 104, 364, 956, 1083
BstMBI GATC 6 cut(s) 115, 331, 526, 665, 877, 1306
BstMWI GCNNNNNNNGC 3 cut(s) 61, 70, 154
BstNI CCWGG 1 cut(s) 1385
BstNSI RCATGY 1 cut(s) 564
BstSCI CCNGG 1 cut(s) 1383
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 4 cut(s) 39, 67, 340, 1227
BstV2I GAAGAC 1 cut(s) 1371
BstX2I RGATCY 2 cut(s) 115, 1306
BstYI RGATCY 2 cut(s) 115, 1306
Bsu36I CCTNAGG 1 cut(s) 1227
BsuI GTATCC 1 cut(s) 904
BsuRI GGCC 3 cut(s) 419, 749, 991
BtgI CCRYGG 3 cut(s) 427, 906, 1092
BtsCI GGATG 6 cut(s) 170, 667, 902, 1063, 1081, 1215
BtsI GCAGTG 2 cut(s) 1114, 1253
BtsIMutI CAGTG 5 cut(s) 174, 972, 1114, 1253, 1291
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 3 cut(s) 25, 71, 1105
CfoI GCGC 1 cut(s) 437
Cfr13I GGNCC 2 cut(s) 827, 989
CseI GACGC 1 cut(s) 441
Csp6I GTAC 2 cut(s) 1097, 1213
CspCI CAANNNNNGTGG 2 cut(s) 693, 728
CviAII CATG 6 cut(s) 316, 561, 847, 907, 1042, 1093
CviQI GTAC 2 cut(s) 1097, 1213
DdeI CTNAG 7 cut(s) 111, 378, 530, 960, 1170, 1200, 1227
DpnI GATC 6 cut(s) 117, 333, 528, 667, 879, 1308
DpnII GATC 6 cut(s) 115, 331, 526, 665, 877, 1306
DrdI GACNNNNNNGTC 1 cut(s) 38
DriI GACNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 1 cut(s) 417
Eam1105I GACNNNNNGTC 1 cut(s) 134
EciI GGCGGA 2 cut(s) 67, 1279
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 3 cut(s) 906, 1092, 1194
Eco24I GRGCYC 3 cut(s) 75, 466, 544
Eco47I GGWCC 1 cut(s) 827
Eco53kI GAGCTC 1 cut(s) 73
Eco57I CTGAAG 2 cut(s) 909, 1335
Eco81I CCTNAGG 1 cut(s) 1227
EcoICRI GAGCTC 1 cut(s) 73
EcoO109I RGGNCCY 1 cut(s) 827
EcoRII CCWGG 1 cut(s) 1383
EcoT14I CCWWGG 3 cut(s) 906, 1092, 1194
EcoT38I GRGCYC 3 cut(s) 75, 466, 544
ErhI CCWWGG 3 cut(s) 906, 1092, 1194
Esp3I CGTCTC 1 cut(s) 104
FaeI CATG 6 cut(s) 319, 564, 850, 910, 1045, 1096
FalI AAGNNNNNCTT 2 cut(s) 935, 967
FatI CATG 6 cut(s) 315, 560, 846, 906, 1041, 1092
FbaI TGATCA 1 cut(s) 877
Fnu4HI GCNGC 5 cut(s) 28, 56, 230, 354, 1241
FokI GGATG 6 cut(s) 177, 674, 889, 1068, 1070, 1222
FriOI GRGCYC 3 cut(s) 75, 466, 544
Fsp4HI GCNGC 5 cut(s) 28, 56, 230, 354, 1241
FspBI CTAG 2 cut(s) 1151, 1310
GlaI GCGC 1 cut(s) 436
GluI GCNGC 5 cut(s) 28, 56, 230, 354, 1241
GsaI CCCAGC 2 cut(s) 68, 461
GsuI CTGGAG 1 cut(s) 1363
HaeIII GGCC 3 cut(s) 419, 749, 991
HgaI GACGC 1 cut(s) 441
HhaI GCGC 1 cut(s) 437
Hin1II CATG 6 cut(s) 319, 564, 850, 910, 1045, 1096
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HincII GTYRAC 1 cut(s) 683
HindII GTYRAC 1 cut(s) 683
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 4 cut(s) 893, 1033, 1138, 1346
HphI GGTGA 5 cut(s) 86, 379, 499, 872, 1037
Hpy166II GTNNAC 8 cut(s) 139, 172, 431, 683, 940, 979, 1066, 1137
Hpy188I TCNGA 9 cut(s) 112, 220, 381, 533, 673, 757, 889, 961, 1173
Hpy188III TCNNGA 4 cut(s) 1151, 1229, 1310, 1353
Hpy8I GTNNAC 8 cut(s) 139, 172, 431, 683, 940, 979, 1066, 1137
HpyAV CCTTC 2 cut(s) 576, 1040
HpyCH4III ACNGT 7 cut(s) 98, 178, 428, 928, 967, 1070, 1286
HpyCH4V TGCA 8 cut(s) 191, 501, 614, 620, 650, 1103, 1243, 1258
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 70, 154
HpyF3I CTNAG 7 cut(s) 111, 378, 530, 960, 1170, 1200, 1227
Hsp92II CATG 6 cut(s) 319, 564, 850, 910, 1045, 1096
HspAI GCGC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 6 cut(s) 115, 331, 526, 665, 877, 1306
LmnI GCTCC 2 cut(s) 461, 547
Lsp1109I GCAGC 4 cut(s) 39, 67, 340, 1227
LweI GCATC 2 cut(s) 1227, 1321
MaeI CTAG 2 cut(s) 1151, 1310
MaeIII GTNAC 4 cut(s) 343, 505, 587, 947
MalI GATC 6 cut(s) 117, 333, 528, 667, 879, 1308
MboI GATC 6 cut(s) 115, 331, 526, 665, 877, 1306
MboII GAAGA 5 cut(s) 502, 902, 1341, 1373, 1376
MfeI CAATTG 2 cut(s) 615, 621
MflI RGATCY 2 cut(s) 115, 1306
MhlI GDGCHC 4 cut(s) 75, 466, 544, 1284
MlsI TGGCCA 1 cut(s) 419
MluCI AATT 5 cut(s) 615, 621, 735, 1113, 1232
MluNI TGGCCA 1 cut(s) 419
MlyI GAGTC 1 cut(s) 1132
MmeI TCCRAC 1 cut(s) 712
Mox20I TGGCCA 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 695
MscI TGGCCA 1 cut(s) 419
MseI TTAA 2 cut(s) 264, 1128
MslI CAYNNNNRTG 2 cut(s) 714, 845
Msp20I TGGCCA 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 64
MspR9I CCNGG 1 cut(s) 1385
MunI CAATTG 2 cut(s) 615, 621
MvaI CCWGG 1 cut(s) 1385
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 3 cut(s) 61, 70, 154
NcoI CCATGG 2 cut(s) 906, 1092
NdeII GATC 6 cut(s) 115, 331, 526, 665, 877, 1306
NlaIII CATG 6 cut(s) 319, 564, 850, 910, 1045, 1096
NlaIV GGNNCC 2 cut(s) 463, 543
NmuCI GTSAC 3 cut(s) 343, 505, 947
NspI RCATGY 1 cut(s) 564
PaqCI CACCTGC 1 cut(s) 355
PdmI GAANNNNTTC 1 cut(s) 695
PfeI GAWTC 3 cut(s) 893, 1033, 1346
PkrI GCNGC 5 cut(s) 29, 57, 231, 355, 1242
PleI GAGTC 1 cut(s) 1132
PpsI GAGTC 1 cut(s) 1132
PpuMI RGGWCCY 1 cut(s) 827
Psp124BI GAGCTC 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 827
Psp6I CCWGG 1 cut(s) 1383
PspFI CCCAGC 2 cut(s) 64, 457
PspGI CCWGG 1 cut(s) 1383
PspN4I GGNNCC 2 cut(s) 463, 543
PspPI GGNCC 2 cut(s) 827, 989
PspPPI RGGWCCY 1 cut(s) 827
PsuI RGATCY 2 cut(s) 115, 1306
PvuII CAGCTG 1 cut(s) 64
RsaI GTAC 2 cut(s) 1098, 1214
RsaNI GTAC 2 cut(s) 1097, 1213
RseI CAYNNNNRTG 2 cut(s) 714, 845
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 2 cut(s) 264, 1128
SatI GCNGC 5 cut(s) 28, 56, 230, 354, 1241
Sau3AI GATC 6 cut(s) 115, 331, 526, 665, 877, 1306
Sau96I GGNCC 2 cut(s) 827, 989
SchI GAGTC 1 cut(s) 1132
ScrFI CCNGG 1 cut(s) 1385
SduI GDGCHC 4 cut(s) 75, 466, 544, 1284
SfaNI GCATC 2 cut(s) 1227, 1321
SinI GGWCC 1 cut(s) 827
SmiMI CAYNNNNRTG 2 cut(s) 714, 845
SmlI CTYRAG 1 cut(s) 933
SmoI CTYRAG 1 cut(s) 933
Sse9I AATT 5 cut(s) 615, 621, 735, 1113, 1232
SsiI CCGC 4 cut(s) 78, 229, 1216, 1264
SspMI CTAG 2 cut(s) 1151, 1310
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 1383
StyI CCWWGG 3 cut(s) 906, 1092, 1194
TaaI ACNGT 7 cut(s) 98, 178, 428, 928, 967, 1070, 1286
TaqI TCGA 2 cut(s) 127, 334
TasI AATT 5 cut(s) 615, 621, 735, 1113, 1232
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 3 cut(s) 893, 1033, 1346
Tru1I TTAA 2 cut(s) 264, 1128
Tru9I TTAA 2 cut(s) 264, 1128
TscAI CASTG 5 cut(s) 181, 972, 1114, 1260, 1291
TseFI GTSAC 3 cut(s) 343, 505, 947
TseI GCWGC 4 cut(s) 27, 55, 353, 1240
Tsp45I GTSAC 3 cut(s) 343, 505, 947
TspDTI ATGAA 5 cut(s) 20, 304, 399, 784, 933
TspRI CASTG 5 cut(s) 181, 972, 1114, 1260, 1291
VpaK11BI GGWCC 1 cut(s) 827
XbaI TCTAGA 2 cut(s) 1150, 1309
XceI RCATGY 1 cut(s) 564
XcmI CCANNNNNNNNNTGG 1 cut(s) 572
XmnI GAANNNNTTC 1 cut(s) 695
XspI CTAG 2 cut(s) 1151, 1310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.