RchiOBHm_Chr6g0279071

DDB1- and CUL4-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006022
Physical Location & Seq
Reverse (-)
42257181 .. 42260750
3570 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25032

Sequence Viewer

Length: 1566 bp
ATGATGAATAAGCGATTGAAAACGAGCAGCGACAAGGCTGTCGTCAATGTTTGGCAGCGACAGCTGGGCGAGCTCTCCGCCAGAAACTTTGCTCACCGTCTCGGTGGCTCTGAGGATCTCGTGCTTCGACTTGATGTCCACAGGAAGCTGGACAAGCATAGAGGATGTGTGAACACTGTAAGCTTCAATGCTGATGGCGATATTTTGCTTTCGGGTTCTGATGACAGGCGGCTCATACTATGGAACTGGGAAACGGGGAAAGTTAAGTTGTCATTCCATTCCGGTCATAATAGCAATGTTTTCCAAGCAAAGTTCATGCCTTATAGTGATGATCGAAGCATTGTCACTTGTGCTGCTGATGGGCAGGTGAGACATACTCAGATTTTAGAACGAGGACAAGTGGAATGTTCAATGTTGGCCAGACACCGTGGACGCGCTCATAAGTTAGCTATTGAGCCTGGGAGCCCTCATATTTTCTATACCTGTGGTGAAGATGGACTGGTGCAACATTTTGATCTCAGAACTGGGGCTCCCACAAAACTTTTGACATGCCAAACACTTGATGGTAGAAGGAGTTACTTGCCAGTTATCAGTCTAAATGCAATTGCAATTGACCCAAGAAATCCAAATCTCTTTGCAGTCGCAGGTTCTGATGAGTATACCCGGCTTTTTGATATCCGCAAGTATAACTGGGATGGATCATCTGATTTTGGTCAACCCACGAACTACTTCTGCCCACCACATTTGAATGGTAATGACCAAGTTGGAATTACAGGCTTGGCCTTTTCAGAACAAAGTGAGCTTCTTGTATCATACAATGACGAGTTCATCTATCTTTTTACACGAGAAATGGGATTAGGACCTAATCCTCCCATTTCCATGAGTAGTGATACAAGTGAAATGAGTGATGATCACCAGTCTGAAGAATCTCCATCATCCATGGATACTGATGAAAAAACTGTTCCTCAAGTTTACAAGGGTCACTTGAACTCCGAGACAGTGAAAGGTGTGAACTTCTTTGGGCCGAAATGCGAGTATGTTGTGAGTGGGTCTGACTGTGGGCGGATATTCATATGGAAGAAAAAGGGTGGTGAGTTGATTCGTGTCATGGAAGGAGATAAGGATGTAGTAAACTGTATCAAGTCTCATCCTCACACCATGGTACTTGCAAGCAGTGGAATTGAAAATGATATTAAGATGTGGACTCCAAAGGCTCTAGAGAGAGCTACTCTGCCTCAGAACATTGAACAGGTCTCGATACCTGGCCGATTAATATGGTACACTATTGGTGATGAGGATGAGGATGATGAATATTCTTATGATGACTATATGTTTGATGACGATGATGATGACAACGACGAGAACGATGAAGATGATGAGGACGAGGATGACGATGACAATGATAGTGATGATCAGGACGAGGATGACAATGATAGTGATGATCAGGATGAGGATGATGGTGGTGGTGATGGTGACAATGTTGAGAATGAAAATGGCAGAAACTGTGAAGATGATGATGATGCTAACAATCGTGATAGTTTTCTTGATGGTTATGATCTACACTAA

Protein Analysis

521

Amino Acids

58.67

Weight (kDa)

4.39

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 5 - 130 2.2e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 43 - 134 1.8e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 45 - 183 1.4e-15 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 46 - 171 7.7e-13 WDR3 first beta-propeller domain
WD40 PF00400 48 - 82 1e-06 WD domain, G-beta repeat
WDR55 PF24796 49 - 174 1.1e-07 WDR55
Beta-prop_WDR3_2nd PF25172 49 - 170 7.6e-06 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 50 - 226 4.7e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 53 - 124 4.4e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 57 - 265 5.8e-12 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 315 - 401 1e-13 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 355
AasI GACNNNNNNGTC 1 cut(s) 38
Acc36I ACCTGC 2 cut(s) 355, 635
AccI GTMKAC 1 cut(s) 659
AccII CGCG 1 cut(s) 435
AciI CCGC 4 cut(s) 78, 229, 679, 1063
AclWI GGATC 2 cut(s) 123, 706
AcoI YGGCCR 2 cut(s) 417, 1264
AcuI CTGAAG 1 cut(s) 942
AfaI GTAC 2 cut(s) 1164, 1280
AgsI TTSAA 7 cut(s) 19, 187, 411, 748, 988, 1184, 1247
AhdI GACNNNNNGTC 1 cut(s) 134
AjnI CCWGG 2 cut(s) 457, 1261
AloI GAACNNNNNNTCC 2 cut(s) 514, 546
AluBI AGCT 7 cut(s) 64, 73, 148, 183, 449, 802, 1226
AluI AGCT 7 cut(s) 64, 73, 148, 183, 449, 802, 1226
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 5 cut(s) 104, 364, 989, 1149, 1258
AlwI GGATC 2 cut(s) 123, 706
AlwNI CAGNNNCTG 2 cut(s) 650, 1503
AoxI GGCC 4 cut(s) 417, 780, 1022, 1264
ApeKI GCWGC 3 cut(s) 27, 55, 353
ArsI GACNNNNNNTTYG 2 cut(s) 1316, 1348
AseI ATTAAT 1 cut(s) 1271
Asp700I GAANNNNTTC 1 cut(s) 728
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 2 cut(s) 860, 1022
AsuC2I CCSGG 1 cut(s) 664
AsuHPI GGTGA 8 cut(s) 86, 379, 500, 905, 1103, 1301, 1478, 1484
AvaII GGWCC 1 cut(s) 860
BalI TGGCCA 1 cut(s) 419
BanII GRGCYC 3 cut(s) 75, 467, 532
BauI CACGAG 2 cut(s) 119, 843
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 3 cut(s) 39, 67, 340
BccI CCATC 9 cut(s) 188, 353, 488, 557, 689, 940, 1451, 1463, 1541
BciT130I CCWGG 2 cut(s) 459, 1263
BciVI GTATCC 1 cut(s) 937
BclI TGATCA 3 cut(s) 910, 1411, 1441
BcnI CCSGG 1 cut(s) 664
BcoDI GTCTC 5 cut(s) 104, 364, 989, 1149, 1258
BfaI CTAG 1 cut(s) 1217
BfuAI ACCTGC 2 cut(s) 355, 635
BfuI GTATCC 1 cut(s) 937
BisI GCNGC 4 cut(s) 28, 56, 230, 354
BlsI GCNGC 4 cut(s) 29, 57, 231, 355
Bme1390I CCNGG 3 cut(s) 459, 664, 1263
Bme18I GGWCC 1 cut(s) 860
BmeRI GACNNNNNGTC 1 cut(s) 134
BmgT120I GGNCC 2 cut(s) 860, 1022
BmiI GGNNCC 2 cut(s) 464, 531
BmrFI CCNGG 3 cut(s) 459, 664, 1263
BmrI ACTGGG 3 cut(s) 256, 534, 700
BmsI GCATC 1 cut(s) 1510
BmuI ACTGGG 3 cut(s) 256, 534, 700
BplI GAGNNNNNCTC 4 cut(s) 361, 393, 1213, 1245
BpuEI CTTGAG 1 cut(s) 951
BpuMI CCSGG 1 cut(s) 664
BsaI GGTCTC 1 cut(s) 1258
BsaJI CCNNGG 4 cut(s) 427, 458, 939, 1158
BsaWI WCCGGW 1 cut(s) 281
BsaXI ACNNNNNCTCC 4 cut(s) 514, 544, 974, 1004
Bse1I ACTGG 6 cut(s) 251, 504, 529, 584, 695, 916
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 2 cut(s) 459, 1263
BseDI CCNNGG 4 cut(s) 427, 458, 939, 1158
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 4 cut(s) 102, 392, 532, 1250
BseNI ACTGG 6 cut(s) 251, 504, 529, 584, 695, 916
BseXI GCAGC 3 cut(s) 39, 67, 340
BseYI CCCAGC 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 4 cut(s) 419, 782, 1024, 1266
BsiHKAI GWGCWC 1 cut(s) 75
BsiSI CCGG 2 cut(s) 282, 664
BsmAI GTCTC 5 cut(s) 104, 364, 989, 1149, 1258
BsmBI CGTCTC 1 cut(s) 104
BsnI GGCC 4 cut(s) 419, 782, 1024, 1266
Bso31I GGTCTC 1 cut(s) 1258
Bsp1286I GDGCHC 3 cut(s) 75, 467, 532
Bsp143I GATC 8 cut(s) 115, 331, 514, 698, 910, 1411, 1441, 1555
Bsp19I CCATGG 2 cut(s) 939, 1158
BspACI CCGC 4 cut(s) 78, 229, 679, 1063
BspANI GGCC 4 cut(s) 419, 782, 1024, 1266
BspCNI CTCAG 4 cut(s) 103, 391, 531, 1249
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 2 cut(s) 464, 531
BspMI ACCTGC 2 cut(s) 355, 635
BspPI GGATC 2 cut(s) 123, 706
BspTNI GGTCTC 1 cut(s) 1258
BsrDI GCAATG 1 cut(s) 301
BsrI ACTGG 6 cut(s) 251, 504, 529, 584, 695, 916
BssECI CCNNGG 4 cut(s) 427, 458, 939, 1158
BssMI GATC 8 cut(s) 115, 331, 514, 698, 910, 1411, 1441, 1555
BssNAI GTATAC 1 cut(s) 660
BssSI CACGAG 2 cut(s) 119, 843
BssT1I CCWWGG 2 cut(s) 939, 1158
Bst1107I GTATAC 1 cut(s) 660
Bst2BI CACGAG 2 cut(s) 119, 843
Bst2UI CCWGG 2 cut(s) 459, 1263
Bst4CI ACNGT 8 cut(s) 98, 178, 428, 961, 1000, 1058, 1136, 1505
BstC8I GCNNGC 2 cut(s) 71, 1171
BstDEI CTNAG 4 cut(s) 111, 378, 518, 1236
BstDSI CCRYGG 3 cut(s) 427, 939, 1158
BstFNI CGCG 1 cut(s) 435
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 8 cut(s) 118, 334, 517, 701, 913, 1414, 1444, 1558
BstMAI GTCTC 5 cut(s) 104, 364, 989, 1149, 1258
BstMBI GATC 8 cut(s) 115, 331, 514, 698, 910, 1411, 1441, 1555
BstMWI GCNNNNNNNGC 3 cut(s) 61, 70, 154
BstNI CCWGG 2 cut(s) 459, 1263
BstNSI RCATGY 1 cut(s) 552
BstSCI CCNGG 3 cut(s) 457, 662, 1261
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 3 cut(s) 39, 67, 340
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BstZ17I GTATAC 1 cut(s) 660
BsuI GTATCC 1 cut(s) 937
BsuRI GGCC 4 cut(s) 419, 782, 1024, 1266
BtgI CCRYGG 3 cut(s) 427, 939, 1158
BtsI GCAGTG 1 cut(s) 1180
BtsIMutI CAGTG 3 cut(s) 174, 1005, 1180
BveI ACCTGC 2 cut(s) 355, 635
Cac8I GCNNGC 2 cut(s) 71, 1171
CaiI CAGNNNCTG 2 cut(s) 650, 1503
CfoI GCGC 1 cut(s) 437
Cfr13I GGNCC 2 cut(s) 860, 1022
CseI GACGC 1 cut(s) 441
Csp6I GTAC 2 cut(s) 1163, 1279
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 6 cut(s) 316, 549, 880, 940, 1108, 1159
CviQI GTAC 2 cut(s) 1163, 1279
DdeI CTNAG 4 cut(s) 111, 378, 518, 1236
DpnI GATC 8 cut(s) 117, 333, 516, 700, 912, 1413, 1443, 1557
DpnII GATC 8 cut(s) 115, 331, 514, 698, 910, 1411, 1441, 1555
DrdI GACNNNNNNGTC 1 cut(s) 38
DriI GACNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 2 cut(s) 417, 1264
Eam1105I GACNNNNNGTC 1 cut(s) 134
EciI GGCGGA 2 cut(s) 67, 1078
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 2 cut(s) 939, 1158
Eco24I GRGCYC 3 cut(s) 75, 467, 532
Eco31I GGTCTC 1 cut(s) 1258
Eco32I GATATC 1 cut(s) 676
Eco47I GGWCC 1 cut(s) 860
Eco53kI GAGCTC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 942
EcoICRI GAGCTC 1 cut(s) 73
EcoO109I RGGNCCY 1 cut(s) 860
EcoRII CCWGG 2 cut(s) 457, 1261
EcoRV GATATC 1 cut(s) 676
EcoT14I CCWWGG 2 cut(s) 939, 1158
EcoT38I GRGCYC 3 cut(s) 75, 467, 532
ErhI CCWWGG 2 cut(s) 939, 1158
Esp3I CGTCTC 1 cut(s) 104
FaeI CATG 6 cut(s) 319, 552, 883, 943, 1111, 1162
FalI AAGNNNNNCTT 2 cut(s) 968, 1000
FatI CATG 6 cut(s) 315, 548, 879, 939, 1107, 1158
FauNDI CATATG 1 cut(s) 1073
FbaI TGATCA 3 cut(s) 910, 1411, 1441
FblI GTMKAC 1 cut(s) 659
Fnu4HI GCNGC 4 cut(s) 28, 56, 230, 354
FriOI GRGCYC 3 cut(s) 75, 467, 532
Fsp4HI GCNGC 4 cut(s) 28, 56, 230, 354
FspBI CTAG 1 cut(s) 1217
GlaI GCGC 1 cut(s) 436
GluI GCNGC 4 cut(s) 28, 56, 230, 354
GsaI CCCAGC 1 cut(s) 68
HaeIII GGCC 4 cut(s) 419, 782, 1024, 1266
HapII CCGG 2 cut(s) 282, 664
HgaI GACGC 1 cut(s) 441
HhaI GCGC 1 cut(s) 437
Hin1II CATG 6 cut(s) 319, 552, 883, 943, 1111, 1162
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HincII GTYRAC 1 cut(s) 716
HindII GTYRAC 1 cut(s) 716
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 3 cut(s) 926, 1099, 1204
HpaII CCGG 2 cut(s) 282, 664
HphI GGTGA 8 cut(s) 86, 379, 500, 905, 1103, 1301, 1478, 1484
Hpy188III TCNNGA 6 cut(s) 1217, 1255, 1415, 1445, 1532, 1544
Hpy99I CGWCG 1 cut(s) 1361
HpyAV CCTTC 2 cut(s) 564, 1106
HpyCH4III ACNGT 8 cut(s) 98, 178, 428, 961, 1000, 1058, 1136, 1505
HpyCH4V TGCA 5 cut(s) 505, 602, 608, 638, 1169
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 70, 154
HpyF3I CTNAG 4 cut(s) 111, 378, 518, 1236
Hsp92II CATG 6 cut(s) 319, 552, 883, 943, 1111, 1162
HspAI GCGC 1 cut(s) 435
Ksp22I TGATCA 3 cut(s) 910, 1411, 1441
Kzo9I GATC 8 cut(s) 115, 331, 514, 698, 910, 1411, 1441, 1555
LmnI GCTCC 2 cut(s) 462, 535
Lsp1109I GCAGC 3 cut(s) 39, 67, 340
LweI GCATC 1 cut(s) 1510
MaeI CTAG 1 cut(s) 1217
MaeIII GTNAC 4 cut(s) 343, 575, 980, 1472
MalI GATC 8 cut(s) 117, 333, 516, 700, 912, 1413, 1443, 1557
MboI GATC 8 cut(s) 115, 331, 514, 698, 910, 1411, 1441, 1555
MboII GAAGA 5 cut(s) 503, 935, 1090, 1382, 1520
MfeI CAATTG 2 cut(s) 603, 609
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 3 cut(s) 75, 467, 532
MlsI TGGCCA 1 cut(s) 419
MluCI AATT 4 cut(s) 603, 609, 768, 1179
MluNI TGGCCA 1 cut(s) 419
MlyI GAGTC 1 cut(s) 1198
MmeI TCCRAC 1 cut(s) 745
Mox20I TGGCCA 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 728
MscI TGGCCA 1 cut(s) 419
MseI TTAA 3 cut(s) 264, 1194, 1271
MslI CAYNNNNRTG 2 cut(s) 747, 878
Msp20I TGGCCA 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 64
MspI CCGG 2 cut(s) 282, 664
MspR9I CCNGG 3 cut(s) 459, 664, 1263
MunI CAATTG 2 cut(s) 603, 609
MvaI CCWGG 2 cut(s) 459, 1263
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 3 cut(s) 61, 70, 154
NciI CCSGG 1 cut(s) 664
NcoI CCATGG 2 cut(s) 939, 1158
NdeI CATATG 1 cut(s) 1073
NdeII GATC 8 cut(s) 115, 331, 514, 698, 910, 1411, 1441, 1555
NlaIII CATG 6 cut(s) 319, 552, 883, 943, 1111, 1162
NlaIV GGNNCC 2 cut(s) 464, 531
NmuCI GTSAC 3 cut(s) 343, 980, 1472
NspI RCATGY 1 cut(s) 552
PaqCI CACCTGC 1 cut(s) 355
PcsI WCGNNNNNNNCGW 2 cut(s) 1362, 1389
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 2 cut(s) 926, 1099
PkrI GCNGC 4 cut(s) 29, 57, 231, 355
PleI GAGTC 1 cut(s) 1198
PpsI GAGTC 1 cut(s) 1198
PpuMI RGGWCCY 1 cut(s) 860
PshBI ATTAAT 1 cut(s) 1271
Psp124BI GAGCTC 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 860
Psp6I CCWGG 2 cut(s) 457, 1261
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 2 cut(s) 457, 1261
PspN4I GGNNCC 2 cut(s) 464, 531
PspPI GGNCC 2 cut(s) 860, 1022
PspPPI RGGWCCY 1 cut(s) 860
PstNI CAGNNNCTG 2 cut(s) 650, 1503
PsuI RGATCY 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 64
RsaI GTAC 2 cut(s) 1164, 1280
RsaNI GTAC 2 cut(s) 1163, 1279
RseI CAYNNNNRTG 2 cut(s) 747, 878
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 3 cut(s) 264, 1194, 1271
SatI GCNGC 4 cut(s) 28, 56, 230, 354
Sau3AI GATC 8 cut(s) 115, 331, 514, 698, 910, 1411, 1441, 1555
Sau96I GGNCC 2 cut(s) 860, 1022
SchI GAGTC 1 cut(s) 1198
ScrFI CCNGG 3 cut(s) 459, 664, 1263
SduI GDGCHC 3 cut(s) 75, 467, 532
SfaNI GCATC 1 cut(s) 1510
SinI GGWCC 1 cut(s) 860
SmiMI CAYNNNNRTG 2 cut(s) 747, 878
SmlI CTYRAG 1 cut(s) 966
SmoI CTYRAG 1 cut(s) 966
Sse9I AATT 4 cut(s) 603, 609, 768, 1179
SsiI CCGC 4 cut(s) 78, 229, 679, 1063
SspI AATATT 1 cut(s) 1313
SspMI CTAG 1 cut(s) 1217
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 3 cut(s) 457, 662, 1261
StyI CCWWGG 2 cut(s) 939, 1158
TaaI ACNGT 8 cut(s) 98, 178, 428, 961, 1000, 1058, 1136, 1505
TaqI TCGA 3 cut(s) 127, 334, 1256
TasI AATT 4 cut(s) 603, 609, 768, 1179
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 2 cut(s) 926, 1099
Tru1I TTAA 3 cut(s) 264, 1194, 1271
Tru9I TTAA 3 cut(s) 264, 1194, 1271
TscAI CASTG 3 cut(s) 181, 1005, 1180
TseFI GTSAC 3 cut(s) 343, 980, 1472
TseI GCWGC 3 cut(s) 27, 55, 353
Tsp45I GTSAC 3 cut(s) 343, 980, 1472
TspDTI ATGAA 8 cut(s) 20, 304, 817, 966, 1060, 1323, 1383, 1503
TspRI CASTG 3 cut(s) 181, 1005, 1180
VpaK11BI GGWCC 1 cut(s) 860
VspI ATTAAT 1 cut(s) 1271
XbaI TCTAGA 1 cut(s) 1216
XceI RCATGY 1 cut(s) 552
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 659
XmnI GAANNNNTTC 1 cut(s) 728
XspI CTAG 1 cut(s) 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.