AT4G38480

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006022
Physical Location & Seq
Forward (+)
18003852 .. 18006283
2432 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G38480.2

Sequence Viewer

Length: 1416 bp
ATGAGTGGAAGGCCGGCGAAAAGGCCGAGGACCGACCATTCCGTCGTGAATGTCTGGGAAAGGGAGCTGGGTCTCTTACCCAATCGCAGTTTCTCAAACCGTTTTTCAGCATCCGAGGATCTATTGCGGCGTCTTGGACTTGACAAGAAGCTTGATAAGCATAAGGGATGTGTAAACACAGTGAGTTTCAATGCTGATGGAGATATCTTGCTTTCTGGTTCTGATGACAGACAAGTTATTCTTTGGGATTGGCAGACTGCTTCTGTCAAACTCTCCTTCGATTCCGGCCACTTCAATAATATTTTCCAAGCCAAATTTATGCCTTTCTCTGACGATCGTACCATTGTCACATCTGCTGCTGATAAGCAGGTCCGCTATTCCAAGATTCTTGAATCTGGACAGGTAGAGACTTCTTTGTTGGGTAAGCATCAAGGACCCGTCCACAAATTGGCTGTTGAGCCTGGAAGTCCTTTCTCATTCTATACTTGTGGTGAGGATGGGGCTGTCAAGCATTTTGATCTTAGGACTCGGGTGGCTACAAATCTTTTCACTTGTAAAGAAGCAAAGTTCAATTTAGTTGTCTACTTGCATGCAATTGCAGTTGATCCTAGGAACCCTGGTCTTCTTGCCGTGGCTGGAATGGATGAGTATGCTCGTGTCTATGATATTCGTAGCTATCGCTCCGAGGGTTGGTATAATTTTACCCAACCCATAGACCACTTTTGTCCTGGCCATTTGATTGGAGATGACCATGTAGGAATAACAGGTCTGGCTTTCTCGGATCAAAGTGAGCTCCTTGCTTCGTATAGTGACGAGTTCATCTATCTTTTCACCCCTGATATGGGGCTTGGCCCAACCCCATATCCATCTTCAACTAAGACTGAGGAGAGGATGACGCCTCAGGTTTATAAAGAGCACACGAACCGTGAAACAGTCAAAGGTGTCAACTTTTTTGGACCCAAGTGTGAGTATGTGGTAAGTGGTTCTGACTGCGGCCGAATATTCATCTGGAGGAAGAAAGACGGAGAGCTTCTCCGTGCCATGGAAGCAGACAGACATGTTGTCAATTGTATAGAGTCTCATCCTCACATGCCACTGATGTGTAGCAGTGGAATAGATACTGATATCAAAATATGGACTCCTGGAGGAACCGAGAAACCTCTGTCGCCAGGAAATGCGAAACAGGCTTCGGGTTTTGGTAACCCACGATGGTTCGACGGATATTATGTGGATGACGATGATGATTCTGACGATGAATCTAGTGAAGAGAGTTCAGATGATGATGATAGTTCGGAAGAGGAGGAGAATGGTGAAGTTGATGTTGAGATTACGAAGGATGATGATAATGATCATGGGGATGATGATGATGATGATGATGAGGATGATGATGGGGATGGGGATGGGGATGAGGGATGA

Protein Analysis

471

Amino Acids

52.61

Weight (kDa)

4.55

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 11 - 163 2.3e-08 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 14 - 150 2.5e-15 WDR5 beta-propeller domain
WD40 PF00400 17 - 50 9.3e-08 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 17 - 140 3.1e-14 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 19 - 191 2.2e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 21 - 93 1.7e-09 THOC3 beta-propeller domain
WD40_Prp19 PF24814 23 - 237 2.3e-14 Prp19 WD40 domain
WD40_Gbeta PF25391 76 - 196 2.9e-06 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 78 - 159 1.2e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 264 - 347 5.8e-12 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 909
AasI GACNNNNNNGTC 1 cut(s) 41
Acc36I ACCTGC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 448
AccI GTMKAC 1 cut(s) 582
AciI CCGC 3 cut(s) 127, 373, 993
AclWI GGATC 3 cut(s) 126, 599, 789
AcoI YGGCCR 3 cut(s) 286, 730, 994
AcsI RAATTY 1 cut(s) 314
AcyI GRCGYC 2 cut(s) 130, 896
AfaI GTAC 1 cut(s) 340
AfiI CCNNNNNNNGG 5 cut(s) 448, 690, 841, 842, 1042
AflIII ACRYGT 1 cut(s) 1057
AgsI TTSAA 5 cut(s) 190, 295, 392, 571, 873
AhdI GACNNNNNGTC 1 cut(s) 1061
AjnI CCWGG 5 cut(s) 460, 616, 727, 1141, 1168
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AleI CACNNNNGTG 1 cut(s) 1099
AluBI AGCT 5 cut(s) 67, 151, 675, 793, 1030
AluI AGCT 5 cut(s) 67, 151, 675, 793, 1030
Alw21I GWGCWC 2 cut(s) 795, 918
Alw26I GTCTC 3 cut(s) 77, 401, 1083
AlwI GGATC 3 cut(s) 126, 599, 789
Ama87I CYCGRG 1 cut(s) 528
AoxI GGCC 6 cut(s) 11, 23, 286, 730, 850, 994
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 1 cut(s) 314
AspA2I CCTAGG 1 cut(s) 608
AspS9I GGNCC 5 cut(s) 30, 370, 434, 851, 956
AsuHPI GGTGA 3 cut(s) 503, 823, 1322
AvaI CYCGRG 1 cut(s) 528
AvaII GGWCC 4 cut(s) 30, 370, 434, 956
AvrII CCTAGG 1 cut(s) 608
AxyI CCTNAGG 1 cut(s) 900
BaeI ACNNNNGTAYC 2 cut(s) 322, 355
BalI TGGCCA 1 cut(s) 732
BanII GRGCYC 1 cut(s) 795
BauI CACGAG 1 cut(s) 654
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 2 cut(s) 795, 918
BbvI GCAGC 1 cut(s) 343
BccI CCATC 7 cut(s) 191, 491, 874, 1203, 1382, 1388, 1394
BceAI ACGGC 1 cut(s) 614
BciT130I CCWGG 5 cut(s) 462, 618, 729, 1143, 1170
BclI TGATCA 1 cut(s) 1348
BcoDI GTCTC 3 cut(s) 77, 401, 1083
BfaI CTAG 2 cut(s) 609, 1260
BfuAI ACCTGC 1 cut(s) 358
BisI GCNGC 3 cut(s) 128, 357, 994
BlnI CCTAGG 1 cut(s) 608
BlsI GCNGC 3 cut(s) 129, 358, 995
Bme1390I CCNGG 5 cut(s) 462, 618, 729, 1143, 1170
Bme18I GGWCC 4 cut(s) 30, 370, 434, 956
BmeRI GACNNNNNGTC 1 cut(s) 1061
BmeT110I CYCGRG 1 cut(s) 528
BmgT120I GGNCC 5 cut(s) 30, 370, 434, 851, 956
BmiI GGNNCC 4 cut(s) 436, 614, 958, 1150
BmrFI CCNGG 5 cut(s) 462, 618, 729, 1143, 1170
BmsI GCATC 2 cut(s) 119, 436
BpiI GAAGAC 1 cut(s) 614
BplI GAGNNNNNCTC 2 cut(s) 1017, 1049
BpmI CTGGAG 2 cut(s) 1030, 1164
BsaBI GATNNNNATC 1 cut(s) 1347
BsaHI GRCGYC 2 cut(s) 130, 896
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 7 cut(s) 26, 114, 608, 616, 630, 684, 1041
Bsc4I CCNNNNNNNGG 5 cut(s) 448, 690, 841, 842, 1042
Bse118I RCCGGY 1 cut(s) 13
Bse21I CCTNAGG 1 cut(s) 900
Bse8I GATNNNNATC 1 cut(s) 1347
BseBI CCWGG 5 cut(s) 462, 618, 729, 1143, 1170
BseDI CCNNGG 7 cut(s) 26, 114, 608, 616, 630, 684, 1041
BseJI GATNNNNATC 1 cut(s) 1347
BseLI CCNNNNNNNGG 5 cut(s) 448, 690, 841, 842, 1042
BseMII CTCAG 2 cut(s) 873, 914
BseRI GAGGAG 3 cut(s) 899, 1313, 1316
BseX3I CGGCCG 1 cut(s) 994
BseXI GCAGC 1 cut(s) 343
BseYI CCCAGC 1 cut(s) 67
Bsh1285I CGRYCG 2 cut(s) 337, 997
BshFI GGCC 6 cut(s) 13, 25, 288, 732, 852, 996
BsiEI CGRYCG 2 cut(s) 337, 997
BsiHKAI GWGCWC 2 cut(s) 795, 918
BsiHKCI CYCGRG 1 cut(s) 528
BsiSI CCGG 2 cut(s) 14, 285
BslI CCNNNNNNNGG 5 cut(s) 448, 690, 841, 842, 1042
BsmAI GTCTC 3 cut(s) 77, 401, 1083
BsnI GGCC 6 cut(s) 13, 25, 288, 732, 852, 996
Bso31I GGTCTC 1 cut(s) 77
BsoBI CYCGRG 1 cut(s) 528
Bsp1286I GDGCHC 2 cut(s) 795, 918
Bsp143I GATC 6 cut(s) 118, 334, 517, 604, 781, 1348
Bsp19I CCATGG 1 cut(s) 1041
BspACI CCGC 3 cut(s) 127, 373, 993
BspANI GGCC 6 cut(s) 13, 25, 288, 732, 852, 996
BspCNI CTCAG 2 cut(s) 874, 913
BspLI GGNNCC 4 cut(s) 436, 614, 958, 1150
BspMI ACCTGC 1 cut(s) 358
BspPI GGATC 3 cut(s) 126, 599, 789
BspTNI GGTCTC 1 cut(s) 77
BsrFI RCCGGY 1 cut(s) 13
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 7 cut(s) 26, 114, 608, 616, 630, 684, 1041
BssMI GATC 6 cut(s) 118, 334, 517, 604, 781, 1348
BssNI GRCGYC 2 cut(s) 130, 896
BssSI CACGAG 1 cut(s) 654
BssT1I CCWWGG 2 cut(s) 608, 1041
Bst2BI CACGAG 1 cut(s) 654
Bst2UI CCWGG 5 cut(s) 462, 618, 729, 1143, 1170
Bst4CI ACNGT 4 cut(s) 101, 181, 926, 934
Bst6I CTCTTC 2 cut(s) 1260, 1290
BstACI GRCGYC 2 cut(s) 130, 896
BstC8I GCNNGC 2 cut(s) 15, 591
BstDEI CTNAG 4 cut(s) 521, 876, 882, 900
BstDSI CCRYGG 2 cut(s) 630, 1041
BstEII GGTNACC 1 cut(s) 1199
BstKTI GATC 6 cut(s) 121, 337, 520, 607, 784, 1351
BstMAI GTCTC 3 cut(s) 77, 401, 1083
BstMBI GATC 6 cut(s) 118, 334, 517, 604, 781, 1348
BstMCI CGRYCG 2 cut(s) 337, 997
BstMWI GCNNNNNNNGC 3 cut(s) 157, 1046, 1184
BstNI CCWGG 5 cut(s) 462, 618, 729, 1143, 1170
BstNSI RCATGY 3 cut(s) 593, 1061, 1093
BstPI GGTNACC 1 cut(s) 1199
BstSCI CCNGG 5 cut(s) 460, 616, 727, 1141, 1168
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 1 cut(s) 118
BstXI CCANNNNNNTGG 1 cut(s) 740
BstYI RGATCY 1 cut(s) 118
BstZI CGGCCG 1 cut(s) 994
Bsu36I CCTNAGG 1 cut(s) 900
BsuRI GGCC 6 cut(s) 13, 25, 288, 732, 852, 996
BtgI CCRYGG 2 cut(s) 630, 1041
BtsI GCAGTG 1 cut(s) 1114
BtsIMutI CAGTG 3 cut(s) 186, 1094, 1114
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 15, 591
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 5 cut(s) 30, 370, 434, 851, 956
CseI GACGC 2 cut(s) 119, 904
Csp6I GTAC 1 cut(s) 339
CviAII CATG 6 cut(s) 590, 752, 1042, 1058, 1090, 1352
CviQI GTAC 1 cut(s) 339
DdeI CTNAG 4 cut(s) 521, 876, 882, 900
DpnI GATC 6 cut(s) 120, 336, 519, 606, 783, 1350
DpnII GATC 6 cut(s) 118, 334, 517, 604, 781, 1348
DrdI GACNNNNNNGTC 1 cut(s) 41
DriI GACNNNNNGTC 1 cut(s) 1061
DseDI GACNNNNNNGTC 1 cut(s) 41
EaeI YGGCCR 3 cut(s) 286, 730, 994
EagI CGGCCG 1 cut(s) 994
Eam1104I CTCTTC 2 cut(s) 1260, 1290
Eam1105I GACNNNNNGTC 1 cut(s) 1061
EarI CTCTTC 2 cut(s) 1260, 1290
Ecl136II GAGCTC 1 cut(s) 793
EclXI CGGCCG 1 cut(s) 994
Eco130I CCWWGG 2 cut(s) 608, 1041
Eco24I GRGCYC 1 cut(s) 795
Eco31I GGTCTC 1 cut(s) 77
Eco32I GATATC 2 cut(s) 205, 1126
Eco47I GGWCC 4 cut(s) 30, 370, 434, 956
Eco52I CGGCCG 1 cut(s) 994
Eco53kI GAGCTC 1 cut(s) 793
Eco81I CCTNAGG 1 cut(s) 900
Eco88I CYCGRG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 1199
EcoICRI GAGCTC 1 cut(s) 793
EcoO109I RGGNCCY 1 cut(s) 434
EcoO65I GGTNACC 1 cut(s) 1199
EcoRII CCWGG 5 cut(s) 460, 616, 727, 1141, 1168
EcoRV GATATC 2 cut(s) 205, 1126
EcoT14I CCWWGG 2 cut(s) 608, 1041
EcoT38I GRGCYC 1 cut(s) 795
ErhI CCWWGG 2 cut(s) 608, 1041
FaeI CATG 6 cut(s) 593, 755, 1045, 1061, 1093, 1355
FalI AAGNNNNNCTT 2 cut(s) 225, 257
FatI CATG 6 cut(s) 589, 751, 1041, 1057, 1089, 1351
FbaI TGATCA 1 cut(s) 1348
FblI GTMKAC 1 cut(s) 582
Fnu4HI GCNGC 3 cut(s) 128, 357, 994
FriOI GRGCYC 1 cut(s) 795
Fsp4HI GCNGC 3 cut(s) 128, 357, 994
FspBI CTAG 2 cut(s) 609, 1260
GluI GCNGC 3 cut(s) 128, 357, 994
GsaI CCCAGC 1 cut(s) 71
GsuI CTGGAG 2 cut(s) 1030, 1164
HaeIII GGCC 6 cut(s) 13, 25, 288, 732, 852, 996
HapII CCGG 2 cut(s) 14, 285
HgaI GACGC 2 cut(s) 119, 904
Hin1I GRCGYC 2 cut(s) 130, 896
Hin1II CATG 6 cut(s) 593, 755, 1045, 1061, 1093, 1355
HincII GTYRAC 1 cut(s) 946
HindII GTYRAC 1 cut(s) 946
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 8 cut(s) 281, 385, 392, 526, 1076, 1138, 1244, 1256
HpaII CCGG 2 cut(s) 14, 285
HphI GGTGA 3 cut(s) 503, 823, 1322
Hpy166II GTNNAC 4 cut(s) 175, 442, 583, 946
Hpy188I TCNGA 9 cut(s) 115, 223, 331, 685, 781, 988, 1249, 1276, 1294
Hpy188III TCNNGA 4 cut(s) 46, 389, 396, 1009
Hpy8I GTNNAC 4 cut(s) 175, 442, 583, 946
Hpy99I CGWCG 2 cut(s) 47, 1220
HpyAV CCTTC 3 cut(s) 3, 286, 1327
HpyCH4III ACNGT 4 cut(s) 101, 181, 926, 934
HpyCH4V TGCA 3 cut(s) 589, 593, 599
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 1046, 1184
HpyF3I CTNAG 4 cut(s) 521, 876, 882, 900
Hsp92I GRCGYC 2 cut(s) 130, 896
Hsp92II CATG 6 cut(s) 593, 755, 1045, 1061, 1093, 1355
KroI GCCGGC 1 cut(s) 13
KroNI GCCGGC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 1348
Kzo9I GATC 6 cut(s) 118, 334, 517, 604, 781, 1348
LmnI GCTCC 3 cut(s) 64, 686, 798
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 2 cut(s) 119, 436
MaeI CTAG 2 cut(s) 609, 1260
MaeIII GTNAC 3 cut(s) 346, 809, 1199
MalI GATC 6 cut(s) 120, 336, 519, 606, 783, 1350
MboI GATC 6 cut(s) 118, 334, 517, 604, 781, 1348
MboII GAAGA 5 cut(s) 614, 861, 1027, 1277, 1307
MfeI CAATTG 2 cut(s) 594, 1066
MflI RGATCY 1 cut(s) 118
MhlI GDGCHC 2 cut(s) 795, 918
MlsI TGGCCA 1 cut(s) 732
MluCI AATT 6 cut(s) 314, 446, 571, 594, 697, 1066
MluNI TGGCCA 1 cut(s) 732
MlyI GAGTC 3 cut(s) 520, 1085, 1132
Mox20I TGGCCA 1 cut(s) 732
MroNI GCCGGC 1 cut(s) 13
MscI TGGCCA 1 cut(s) 732
MslI CAYNNNNRTG 2 cut(s) 1099, 1356
Msp20I TGGCCA 1 cut(s) 732
MspI CCGG 2 cut(s) 14, 285
MspR9I CCNGG 5 cut(s) 462, 618, 729, 1143, 1170
MunI CAATTG 2 cut(s) 594, 1066
MvaI CCWGG 5 cut(s) 462, 618, 729, 1143, 1170
MwoI GCNNNNNNNGC 3 cut(s) 157, 1046, 1184
NaeI GCCGGC 1 cut(s) 15
NcoI CCATGG 1 cut(s) 1041
NdeII GATC 6 cut(s) 118, 334, 517, 604, 781, 1348
NgoMIV GCCGGC 1 cut(s) 13
NlaIII CATG 6 cut(s) 593, 755, 1045, 1061, 1093, 1355
NlaIV GGNNCC 4 cut(s) 436, 614, 958, 1150
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 2 cut(s) 346, 809
NspI RCATGY 3 cut(s) 593, 1061, 1093
OliI CACNNNNGTG 1 cut(s) 1099
PaeI GCATGC 1 cut(s) 593
PciI ACATGT 1 cut(s) 1057
PdiI GCCGGC 1 cut(s) 15
PfeI GAWTC 5 cut(s) 281, 385, 392, 1244, 1256
PflMI CCANNNNNTGG 1 cut(s) 448
PfoI TCCNGGA 1 cut(s) 1141
PkrI GCNGC 3 cut(s) 129, 358, 995
Ple19I CGATCG 1 cut(s) 337
PleI GAGTC 3 cut(s) 520, 1084, 1132
PpsI GAGTC 3 cut(s) 520, 1084, 1132
PpuMI RGGWCCY 1 cut(s) 434
PscI ACATGT 1 cut(s) 1057
PsiI TTATAA 1 cut(s) 909
Psp124BI GAGCTC 1 cut(s) 795
Psp5II RGGWCCY 1 cut(s) 434
Psp6I CCWGG 5 cut(s) 460, 616, 727, 1141, 1168
PspEI GGTNACC 1 cut(s) 1199
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 5 cut(s) 460, 616, 727, 1141, 1168
PspN4I GGNNCC 4 cut(s) 436, 614, 958, 1150
PspPI GGNCC 5 cut(s) 30, 370, 434, 851, 956
PspPPI RGGWCCY 1 cut(s) 434
PsuI RGATCY 1 cut(s) 118
PvuI CGATCG 1 cut(s) 337
RsaI GTAC 1 cut(s) 340
RsaNI GTAC 1 cut(s) 339
RseI CAYNNNNRTG 2 cut(s) 1099, 1356
SacI GAGCTC 1 cut(s) 795
SatI GCNGC 3 cut(s) 128, 357, 994
Sau3AI GATC 6 cut(s) 118, 334, 517, 604, 781, 1348
Sau96I GGNCC 5 cut(s) 30, 370, 434, 851, 956
SchI GAGTC 3 cut(s) 520, 1085, 1132
ScrFI CCNGG 5 cut(s) 462, 618, 729, 1143, 1170
SduI GDGCHC 2 cut(s) 795, 918
SfaNI GCATC 2 cut(s) 119, 436
SinI GGWCC 4 cut(s) 30, 370, 434, 956
SmiMI CAYNNNNRTG 2 cut(s) 1099, 1356
SphI GCATGC 1 cut(s) 593
Sse9I AATT 6 cut(s) 314, 446, 571, 594, 697, 1066
SsiI CCGC 3 cut(s) 127, 373, 993
SspI AATATT 2 cut(s) 301, 1002
SspMI CTAG 2 cut(s) 609, 1260
SstI GAGCTC 1 cut(s) 795
StyD4I CCNGG 5 cut(s) 460, 616, 727, 1141, 1168
StyI CCWWGG 2 cut(s) 608, 1041
TaaI ACNGT 4 cut(s) 101, 181, 926, 934
TaqI TCGA 2 cut(s) 279, 1215
TaqII GACCGA 1 cut(s) 47
TasI AATT 6 cut(s) 314, 446, 571, 594, 697, 1066
TauI GCSGC 2 cut(s) 130, 996
TfiI GAWTC 5 cut(s) 281, 385, 392, 1244, 1256
TscAI CASTG 3 cut(s) 186, 1101, 1114
TseFI GTSAC 2 cut(s) 346, 809
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 2 cut(s) 346, 809
TspDTI ATGAA 3 cut(s) 808, 994, 1269
TspGWI ACGGA 4 cut(s) 31, 1025, 1038, 1233
TspRI CASTG 3 cut(s) 186, 1101, 1114
Van91I CCANNNNNTGG 1 cut(s) 448
VpaK11BI GGWCC 4 cut(s) 30, 370, 434, 956
XapI RAATTY 1 cut(s) 314
XceI RCATGY 3 cut(s) 593, 1061, 1093
XcmI CCANNNNNNNNNTGG 1 cut(s) 725
XmaJI CCTAGG 1 cut(s) 608
XmiI GTMKAC 1 cut(s) 582
XspI CTAG 2 cut(s) 609, 1260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.