Rroxscaffold_7G00189170

DDB1- and CUL4-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006022
Physical Location & Seq
Forward (+)
28910957 .. 28915718
4762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00189170.1

Sequence Viewer

Length: 1467 bp
ATGAATAAGCGATTGAAAACGAGCAGCGACAAGGCTGTCGTCAATGTTTGGCAGCGACAGCTCGGCGAGCTCTCCGCCAGAAACTTTGCTCACCGTCTCGGTGGCTCTGAGGATCTCGTGCTTCGACTTGATGTCCACAGGAAGCTGGATAAGCATAGAGGATGTGTGAACACCGTAAGCTTCAATGCAGATGGTGATATTTTGCTTTCGGGTTCTGATGACAGGCGGCTCATACTATGGAACTGGGAAACGGGGAAAGTTAAGTTGTCATTCCATTCCGGTCATAATAGCAATGTTTTCCAAGCAAAGTTCATGCCTTACAGTGATGATCGAAGCATTGTCACTTGTGCTGCTGATGGACAGGTGAGACATACTCAGATTTTAGAACGAGGACAAGTGGAAAGTTCAATGTTGGCCAGACACCATGGACGCGCTCATAAGTTAGCCATTGAGCCTGGGAGCCCTCATATTTTCTATACCTGTGGTGAAGATGGACTGGTGCAACATTTTGATCTCAGAACTGGGGCTCCCACAAAACTTTTGACATGCCAAACACTTGATGGTAGAAGGAGTTACTTGCCAGTTATCAGTCTAAATGCAATTGCAATTGACCCAAGAAATCCAAATCTCTTTGCAGTCGCAGGTTCTGATGAGTATACCCGGCTTTTTGATATCCGCAAGTATAACTGGGATGGATCATCTGATTTTGGTCAACCCACAAACTACTTCTGCCCACCACATTTGATTGGTAATGACCAAGTTGGAATTACAGGCTTGGCCTTTTCAGAACAAAGTGAGCTTCTTGTATCATACAATGACGAGTCCATCTATCTTTTTACACGAGAAATGGGATTAGGACCTAATCCTCCCATTTCCATGAGTAGTGATGCAAGTGAAATGAGTGATGATCACGAGTCTGAAGAATCTCCATCATCCATGGATACTGATGAAAAAACTGTTCCTCAAGTTTACAAGGGTCACTTGAACTCTGAGACAGTGAAAGGTGTGAACTTCTTTGGGCCGAAATGCGAGTATGTTGTGAGTGGGTCTGACTGTGGGCGGATATTCATATGGAAGAAAAAGGGAGGTGAGTTGATTCGTGTCATGGAAGGAGATAAGGATGTAGTAAACTGTATCAAGTCTCATCCTCACACCATGGTACTTGCAAGCAGTGGAATTGAAAATGATATTAAGATGTGGACTCCAAAGGCTCTAGAGAGAGCTACCCTGCCTCAGAACATTGAACAGCAGAAACCCAAGGCTAAGGGCTGGATGTACCGCTTTTCGCCTCAGGAATTGATGCTGCAACTTTTTTCACTGCAACGGCGGAGGACAAGCCCAGAGCACAGTGGGGAAAACTCATCGGCAGATCTAGAGCTAATACTGACATTAGATGCCAACAATGATGATTCTTCAGGAGAAGAAGACGACGATGACAACTCCAGCCAGGGGGATTTTGTTTTTTGA

Protein Analysis

488

Amino Acids

54.63

Weight (kDa)

5.53

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 4 - 132 1.5e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 42 - 135 8.3e-08 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 44 - 182 9.3e-16 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 45 - 170 2.4e-12 WDR3 first beta-propeller domain
WD40 PF00400 47 - 81 9.4e-07 WD domain, G-beta repeat
WDR55 PF24796 48 - 123 4.2e-06 WDR55
Beta-prop_WDR3_2nd PF25172 48 - 169 3e-06 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 49 - 225 3.9e-08 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 52 - 123 4.3e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 56 - 229 3e-12 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 109 - 184 7.9e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 314 - 400 9e-14 WDR5 beta-propeller domain
WD40_Prp19 PF24814 314 - 421 9.6e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 35
Acc36I ACCTGC 1 cut(s) 632
AccI GTMKAC 1 cut(s) 656
AccII CGCG 1 cut(s) 432
AciI CCGC 6 cut(s) 75, 226, 676, 1060, 1279, 1327
AclWI GGATC 2 cut(s) 120, 703
AcoI YGGCCR 1 cut(s) 414
AcuI CTGAAG 2 cut(s) 939, 1398
AfaI GTAC 2 cut(s) 1161, 1277
AfiI CCNNNNNNNGG 1 cut(s) 1449
AgsI TTSAA 6 cut(s) 16, 184, 408, 985, 1181, 1244
AhdI GACNNNNNGTC 1 cut(s) 131
AjnI CCWGG 2 cut(s) 454, 1446
AloI GAACNNNNNNTCC 2 cut(s) 511, 543
AluBI AGCT 7 cut(s) 61, 70, 145, 180, 799, 1223, 1378
AluI AGCT 7 cut(s) 61, 70, 145, 180, 799, 1223, 1378
Alw21I GWGCWC 2 cut(s) 72, 1347
Alw26I GTCTC 4 cut(s) 101, 361, 986, 1146
AlwI GGATC 2 cut(s) 120, 703
AlwNI CAGNNNCTG 1 cut(s) 647
AoxI GGCC 3 cut(s) 414, 777, 1019
ApeKI GCWGC 4 cut(s) 24, 52, 350, 1303
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 2 cut(s) 857, 1019
AsuC2I CCSGG 1 cut(s) 661
AsuHPI GGTGA 5 cut(s) 83, 206, 376, 497, 1100
AvaII GGWCC 1 cut(s) 857
AxyI CCTNAGG 1 cut(s) 1290
BalI TGGCCA 1 cut(s) 416
BanII GRGCYC 3 cut(s) 72, 464, 529
BauI CACGAG 3 cut(s) 116, 840, 911
BbsI GAAGAC 1 cut(s) 1431
Bbv12I GWGCWC 2 cut(s) 72, 1347
BbvI GCAGC 4 cut(s) 36, 64, 337, 1290
BccI CCATC 7 cut(s) 185, 350, 485, 554, 686, 833, 937
BceAI ACGGC 1 cut(s) 1340
BciT130I CCWGG 2 cut(s) 456, 1448
BciVI GTATCC 1 cut(s) 934
BclI TGATCA 1 cut(s) 907
BcnI CCSGG 1 cut(s) 661
BcoDI GTCTC 4 cut(s) 101, 361, 986, 1146
BfaI CTAG 2 cut(s) 1214, 1373
BfuAI ACCTGC 1 cut(s) 632
BfuI GTATCC 1 cut(s) 934
BglII AGATCT 1 cut(s) 1369
BisI GCNGC 5 cut(s) 25, 53, 227, 351, 1304
BlsI GCNGC 5 cut(s) 26, 54, 228, 352, 1305
Bme1390I CCNGG 3 cut(s) 456, 661, 1448
Bme18I GGWCC 1 cut(s) 857
BmeRI GACNNNNNGTC 1 cut(s) 131
BmgT120I GGNCC 2 cut(s) 857, 1019
BmiI GGNNCC 2 cut(s) 461, 528
BmrFI CCNGG 3 cut(s) 456, 661, 1448
BmrI ACTGGG 3 cut(s) 253, 531, 697
BmsI GCATC 3 cut(s) 877, 1290, 1384
BmuI ACTGGG 3 cut(s) 253, 531, 697
BpiI GAAGAC 1 cut(s) 1431
BplI GAGNNNNNCTC 2 cut(s) 358, 390
BpmI CTGGAG 1 cut(s) 1426
Bpu10I CCTNAGC 1 cut(s) 1263
BpuEI CTTGAG 1 cut(s) 948
BpuMI CCSGG 1 cut(s) 661
BsaJI CCNNGG 6 cut(s) 424, 455, 936, 1155, 1257, 1447
BsaWI WCCGGW 1 cut(s) 278
BsaXI ACNNNNNCTCC 2 cut(s) 511, 541
Bsc4I CCNNNNNNNGG 1 cut(s) 1449
Bse1I ACTGG 5 cut(s) 248, 501, 526, 581, 692
Bse21I CCTNAGG 1 cut(s) 1290
Bse3DI GCAATG 1 cut(s) 298
BseBI CCWGG 2 cut(s) 456, 1448
BseDI CCNNGG 6 cut(s) 424, 455, 936, 1155, 1257, 1447
BseGI GGATG 6 cut(s) 167, 697, 932, 1126, 1144, 1278
BseLI CCNNNNNNNGG 1 cut(s) 1449
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 6 cut(s) 99, 389, 529, 981, 1247, 1304
BseNI ACTGG 5 cut(s) 248, 501, 526, 581, 692
BseXI GCAGC 4 cut(s) 36, 64, 337, 1290
Bsh1236I CGCG 1 cut(s) 432
BshFI GGCC 3 cut(s) 416, 779, 1021
BsiHKAI GWGCWC 2 cut(s) 72, 1347
BsiSI CCGG 2 cut(s) 279, 661
BslI CCNNNNNNNGG 1 cut(s) 1449
BsmAI GTCTC 4 cut(s) 101, 361, 986, 1146
BsmBI CGTCTC 1 cut(s) 101
BsnI GGCC 3 cut(s) 416, 779, 1021
Bsp1286I GDGCHC 4 cut(s) 72, 464, 529, 1347
Bsp143I GATC 6 cut(s) 112, 328, 511, 695, 907, 1369
Bsp19I CCATGG 3 cut(s) 424, 936, 1155
BspACI CCGC 6 cut(s) 75, 226, 676, 1060, 1279, 1327
BspANI GGCC 3 cut(s) 416, 779, 1021
BspCNI CTCAG 6 cut(s) 100, 388, 528, 982, 1246, 1303
BspFNI CGCG 1 cut(s) 432
BspLI GGNNCC 2 cut(s) 461, 528
BspMI ACCTGC 1 cut(s) 632
BspPI GGATC 2 cut(s) 120, 703
BsrDI GCAATG 1 cut(s) 298
BsrI ACTGG 5 cut(s) 248, 501, 526, 581, 692
BssECI CCNNGG 6 cut(s) 424, 455, 936, 1155, 1257, 1447
BssMI GATC 6 cut(s) 112, 328, 511, 695, 907, 1369
BssNAI GTATAC 1 cut(s) 657
BssSI CACGAG 3 cut(s) 116, 840, 911
BssT1I CCWWGG 4 cut(s) 424, 936, 1155, 1257
Bst1107I GTATAC 1 cut(s) 657
Bst2BI CACGAG 3 cut(s) 116, 840, 911
Bst2UI CCWGG 2 cut(s) 456, 1448
Bst4CI ACNGT 8 cut(s) 95, 175, 323, 958, 997, 1055, 1133, 1349
BstC8I GCNNGC 2 cut(s) 68, 1168
BstDEI CTNAG 7 cut(s) 108, 375, 515, 990, 1233, 1263, 1290
BstDSI CCRYGG 3 cut(s) 424, 936, 1155
BstF5I GGATG 6 cut(s) 167, 697, 932, 1126, 1144, 1278
BstFNI CGCG 1 cut(s) 432
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 6 cut(s) 115, 331, 514, 698, 910, 1372
BstMAI GTCTC 4 cut(s) 101, 361, 986, 1146
BstMBI GATC 6 cut(s) 112, 328, 511, 695, 907, 1369
BstMWI GCNNNNNNNGC 3 cut(s) 58, 67, 151
BstNI CCWGG 2 cut(s) 456, 1448
BstNSI RCATGY 1 cut(s) 549
BstSCI CCNGG 3 cut(s) 454, 659, 1446
BstUI CGCG 1 cut(s) 432
BstV1I GCAGC 4 cut(s) 36, 64, 337, 1290
BstV2I GAAGAC 1 cut(s) 1431
BstX2I RGATCY 2 cut(s) 112, 1369
BstYI RGATCY 2 cut(s) 112, 1369
BstZ17I GTATAC 1 cut(s) 657
Bsu36I CCTNAGG 1 cut(s) 1290
BsuI GTATCC 1 cut(s) 934
BsuRI GGCC 3 cut(s) 416, 779, 1021
BtgI CCRYGG 3 cut(s) 424, 936, 1155
BtsCI GGATG 6 cut(s) 167, 697, 932, 1126, 1144, 1278
BtsI GCAGTG 2 cut(s) 1177, 1316
BtsIMutI CAGTG 5 cut(s) 328, 1002, 1177, 1316, 1354
BveI ACCTGC 1 cut(s) 632
Cac8I GCNNGC 2 cut(s) 68, 1168
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 857, 1019
CseI GACGC 1 cut(s) 438
Csp6I GTAC 2 cut(s) 1160, 1276
CspCI CAANNNNNGTGG 2 cut(s) 723, 758
CviAII CATG 7 cut(s) 313, 425, 546, 877, 937, 1105, 1156
CviQI GTAC 2 cut(s) 1160, 1276
DdeI CTNAG 7 cut(s) 108, 375, 515, 990, 1233, 1263, 1290
DpnI GATC 6 cut(s) 114, 330, 513, 697, 909, 1371
DpnII GATC 6 cut(s) 112, 328, 511, 695, 907, 1369
DrdI GACNNNNNNGTC 1 cut(s) 35
DriI GACNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 35
EaeI YGGCCR 1 cut(s) 414
Eam1105I GACNNNNNGTC 1 cut(s) 131
EciI GGCGGA 3 cut(s) 64, 1075, 1342
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 4 cut(s) 424, 936, 1155, 1257
Eco24I GRGCYC 3 cut(s) 72, 464, 529
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 1 cut(s) 857
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 2 cut(s) 939, 1398
Eco81I CCTNAGG 1 cut(s) 1290
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 857
EcoRII CCWGG 2 cut(s) 454, 1446
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 4 cut(s) 424, 936, 1155, 1257
EcoT38I GRGCYC 3 cut(s) 72, 464, 529
ErhI CCWWGG 4 cut(s) 424, 936, 1155, 1257
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 7 cut(s) 316, 428, 549, 880, 940, 1108, 1159
FalI AAGNNNNNCTT 2 cut(s) 965, 997
FatI CATG 7 cut(s) 312, 424, 545, 876, 936, 1104, 1155
FauNDI CATATG 1 cut(s) 1070
FbaI TGATCA 1 cut(s) 907
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 5 cut(s) 25, 53, 227, 351, 1304
FokI GGATG 6 cut(s) 174, 704, 919, 1131, 1133, 1285
FriOI GRGCYC 3 cut(s) 72, 464, 529
Fsp4HI GCNGC 5 cut(s) 25, 53, 227, 351, 1304
FspBI CTAG 2 cut(s) 1214, 1373
GlaI GCGC 1 cut(s) 433
GluI GCNGC 5 cut(s) 25, 53, 227, 351, 1304
GsuI CTGGAG 1 cut(s) 1426
HaeIII GGCC 3 cut(s) 416, 779, 1021
HapII CCGG 2 cut(s) 279, 661
HgaI GACGC 1 cut(s) 438
HhaI GCGC 1 cut(s) 434
Hin1II CATG 7 cut(s) 316, 428, 549, 880, 940, 1108, 1159
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HincII GTYRAC 1 cut(s) 713
HindII GTYRAC 1 cut(s) 713
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 6 cut(s) 821, 914, 923, 1096, 1201, 1409
HpaII CCGG 2 cut(s) 279, 661
HphI GGTGA 5 cut(s) 83, 206, 376, 497, 1100
Hpy166II GTNNAC 8 cut(s) 136, 169, 657, 713, 970, 1009, 1129, 1200
Hpy188III TCNNGA 5 cut(s) 911, 1214, 1292, 1373, 1416
Hpy8I GTNNAC 8 cut(s) 136, 169, 657, 713, 970, 1009, 1129, 1200
Hpy99I CGWCG 1 cut(s) 1433
HpyAV CCTTC 2 cut(s) 561, 1103
HpyCH4III ACNGT 8 cut(s) 95, 175, 323, 958, 997, 1055, 1133, 1349
HpyCH4V TGCA 9 cut(s) 188, 502, 599, 605, 635, 890, 1166, 1306, 1321
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 67, 151
HpyF3I CTNAG 7 cut(s) 108, 375, 515, 990, 1233, 1263, 1290
Hsp92II CATG 7 cut(s) 316, 428, 549, 880, 940, 1108, 1159
HspAI GCGC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 6 cut(s) 112, 328, 511, 695, 907, 1369
LmnI GCTCC 2 cut(s) 459, 532
Lsp1109I GCAGC 4 cut(s) 36, 64, 337, 1290
LweI GCATC 3 cut(s) 877, 1290, 1384
MaeI CTAG 2 cut(s) 1214, 1373
MaeIII GTNAC 3 cut(s) 340, 572, 977
MalI GATC 6 cut(s) 114, 330, 513, 697, 909, 1371
MboI GATC 6 cut(s) 112, 328, 511, 695, 907, 1369
MboII GAAGA 6 cut(s) 500, 932, 1087, 1404, 1433, 1436
MfeI CAATTG 2 cut(s) 600, 606
MflI RGATCY 2 cut(s) 112, 1369
MhlI GDGCHC 4 cut(s) 72, 464, 529, 1347
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 5 cut(s) 600, 606, 765, 1176, 1295
MluNI TGGCCA 1 cut(s) 416
MlyI GAGTC 3 cut(s) 830, 923, 1195
MmeI TCCRAC 1 cut(s) 742
Mox20I TGGCCA 1 cut(s) 416
MscI TGGCCA 1 cut(s) 416
MseI TTAA 2 cut(s) 261, 1191
MslI CAYNNNNRTG 1 cut(s) 875
Msp20I TGGCCA 1 cut(s) 416
MspI CCGG 2 cut(s) 279, 661
MspR9I CCNGG 3 cut(s) 456, 661, 1448
MunI CAATTG 2 cut(s) 600, 606
MvaI CCWGG 2 cut(s) 456, 1448
MvnI CGCG 1 cut(s) 432
MwoI GCNNNNNNNGC 3 cut(s) 58, 67, 151
NciI CCSGG 1 cut(s) 661
NcoI CCATGG 3 cut(s) 424, 936, 1155
NdeI CATATG 1 cut(s) 1070
NdeII GATC 6 cut(s) 112, 328, 511, 695, 907, 1369
NlaIII CATG 7 cut(s) 316, 428, 549, 880, 940, 1108, 1159
NlaIV GGNNCC 2 cut(s) 461, 528
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 2 cut(s) 340, 977
NspI RCATGY 1 cut(s) 549
PfeI GAWTC 3 cut(s) 923, 1096, 1409
PkrI GCNGC 5 cut(s) 26, 54, 228, 352, 1305
PleI GAGTC 3 cut(s) 829, 922, 1195
PpsI GAGTC 3 cut(s) 829, 922, 1195
PpuMI RGGWCCY 1 cut(s) 857
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 857
Psp6I CCWGG 2 cut(s) 454, 1446
PspGI CCWGG 2 cut(s) 454, 1446
PspN4I GGNNCC 2 cut(s) 461, 528
PspPI GGNCC 2 cut(s) 857, 1019
PspPPI RGGWCCY 1 cut(s) 857
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 2 cut(s) 112, 1369
RsaI GTAC 2 cut(s) 1161, 1277
RsaNI GTAC 2 cut(s) 1160, 1276
RseI CAYNNNNRTG 1 cut(s) 875
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 2 cut(s) 261, 1191
SatI GCNGC 5 cut(s) 25, 53, 227, 351, 1304
Sau3AI GATC 6 cut(s) 112, 328, 511, 695, 907, 1369
Sau96I GGNCC 2 cut(s) 857, 1019
SchI GAGTC 3 cut(s) 830, 923, 1195
ScrFI CCNGG 3 cut(s) 456, 661, 1448
SduI GDGCHC 4 cut(s) 72, 464, 529, 1347
SfaNI GCATC 3 cut(s) 877, 1290, 1384
SinI GGWCC 1 cut(s) 857
SmiMI CAYNNNNRTG 1 cut(s) 875
SmlI CTYRAG 1 cut(s) 963
SmoI CTYRAG 1 cut(s) 963
Sse9I AATT 5 cut(s) 600, 606, 765, 1176, 1295
SsiI CCGC 6 cut(s) 75, 226, 676, 1060, 1279, 1327
SspMI CTAG 2 cut(s) 1214, 1373
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 454, 659, 1446
StyI CCWWGG 4 cut(s) 424, 936, 1155, 1257
TaaI ACNGT 8 cut(s) 95, 175, 323, 958, 997, 1055, 1133, 1349
TaqI TCGA 2 cut(s) 124, 331
TasI AATT 5 cut(s) 600, 606, 765, 1176, 1295
TauI GCSGC 1 cut(s) 229
TfiI GAWTC 3 cut(s) 923, 1096, 1409
Tru1I TTAA 2 cut(s) 261, 1191
Tru9I TTAA 2 cut(s) 261, 1191
TscAI CASTG 5 cut(s) 328, 1002, 1177, 1323, 1354
TseFI GTSAC 2 cut(s) 340, 977
TseI GCWGC 4 cut(s) 24, 52, 350, 1303
Tsp45I GTSAC 2 cut(s) 340, 977
TspDTI ATGAA 4 cut(s) 17, 301, 963, 1057
TspRI CASTG 5 cut(s) 328, 1002, 1177, 1323, 1354
VpaK11BI GGWCC 1 cut(s) 857
XbaI TCTAGA 2 cut(s) 1213, 1372
XceI RCATGY 1 cut(s) 549
XcmI CCANNNNNNNNNTGG 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 656
XspI CTAG 2 cut(s) 1214, 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.