AT5G01990

auxin efflux carrier family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003304
Physical Location & Seq
Reverse (-)
377231 .. 379911
2681 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01990.1

Sequence Viewer

Length: 1296 bp
ATGATTGCTCGGATCCTTGCCGCCTTAGCCGATTCCATGGAGATGCCGGTGGCTGCCGGTGGTGGATCAGTGCTCGGTACCATCAAAATCGCTGTGATGCCAATTGCAAAGGTATTCACCATGTGCTTCTTGGGTCTTCTCATGGCCTCCAAGTACGTTAACATCTTGCCTCCCTCTGGCCGCAAACTCTTGAACGGGTTGGTCTTCTCGCTTTTGCTTCCCTGCTTGATCTTTTCCCAGCTCGGACAAGCCGTCACTCTCCAAAAAATGCTTCAGTGGTGGTTCATTCCCGTTAACGTCGTTCTTGGCACCATCTCTGGCTCCATAATCGGTTTCATCGTTGCTTCCATCGTTCGTCCGCCTTACCCTTACTTCAAGTTTACAATCATACAAATTGGAGTTGGCAACATTGGCAATGTGCCTCTTGTTTTACTTGCGGCTCTTTGTAGGGACACTTCCAACCCTTTTGGTGACTCCGAAAAATGTAGCATTGATGGCACTGCTTATATCTCCTTTGGTCAATGGGTTGGTGCCATCATCCTCTATACATATGTGTACCAGATGTTTGCTCCTCCTCCAGAAGGGTTTGATGCTGAAGAAGAAAATCTTGCTTTAAAAACCCTTCCAGTAGATGCTGCTCCAGAGCAAGTCCCCTTGCTTACTCAGAATTTTCCCAAGGACTTTTCGCCGACTCAAGATCTTTTGCCTGTACAGAGCACCGAACCTAGGGGAAGAGGGGTTTCAAGGAAAGGCAAGATTGCACAGATCTTTGTTTTCCTCTATGAGAAGCTGAAACTGAAGCAAATTGTCCAGCCTGCAATTGTTGCTTCGATCCTTGCCATGATACTCGGAGCAATACCTTTCACAAAGAAGTTGATATTCACAAATGGTGCACCTCTATTCTTCTTCACAGACAGCTGCATGATTCTTGGGGACGCGATGATACCGTGTATCTTACTGGCACTGGGGGGAAATCTCATTAACGGACCAGGAAGTTCAAAACTTGGTTTCAAGACAACAGCAGCTATTATAATTGGACGGTTGGTGTTAGTGCCACCAGTAGGACTAGGAATCGTGACAGTAGCAGACAAACTTGGGTTCCTTCCTGCTGATGATAAGATGTTCCGATTTGTGTTACTCCTTCAGCACACAATGCCTACATCAGTGCTCTCAGGAGCTGTTGCCAACCTTAGAGGCTGTGGAAGGGAATCAGCTGCTGTGCTCTTCTGGGTCCACATTTTCGCCATCTTCTCAATGGCTGGATGGATGGTACTCTACATTAACATACTCTTCTGA

Protein Analysis

431

Amino Acids

46.46

Weight (kDa)

9.09

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 31 - 423 1.7e-105 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1031
Acc65I GGTACC 1 cut(s) 77
AccB1I GGYRCC 3 cut(s) 77, 308, 530
AccII CGCG 1 cut(s) 938
AciI CCGC 4 cut(s) 21, 181, 359, 437
AclWI GGATC 4 cut(s) 7, 20, 73, 826
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 1 cut(s) 667
AcuI CTGAAG 4 cut(s) 257, 615, 818, 1127
AdeI CACNNNGTG 1 cut(s) 123
AfaI GTAC 5 cut(s) 79, 155, 557, 711, 1272
AfiI CCNNNNNNNGG 4 cut(s) 176, 581, 726, 1061
AgsI TTSAA 5 cut(s) 193, 376, 744, 999, 1012
AhdI GACNNNNNGTC 1 cut(s) 251
AjnI CCWGG 1 cut(s) 988
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AluBI AGCT 6 cut(s) 241, 790, 918, 1025, 1178, 1214
AluI AGCT 6 cut(s) 241, 790, 918, 1025, 1178, 1214
Alw21I GWGCWC 5 cut(s) 75, 719, 895, 1170, 1224
Alw44I GTGCAC 1 cut(s) 891
AlwI GGATC 4 cut(s) 7, 20, 73, 826
AlwNI CAGNNNCTG 2 cut(s) 1178, 1217
AoxI GGCC 2 cut(s) 144, 178
ApaLI GTGCAC 1 cut(s) 891
ApeKI GCWGC 5 cut(s) 53, 635, 918, 1022, 1214
ApoI RAATTY 1 cut(s) 667
Asp718I GGTACC 1 cut(s) 77
AspA2I CCTAGG 1 cut(s) 725
AspS9I GGNCC 2 cut(s) 986, 1231
AsuHPI GGTGA 2 cut(s) 109, 482
AvaII GGWCC 2 cut(s) 986, 1231
AvrII CCTAGG 1 cut(s) 725
BaeGI GKGCMC 1 cut(s) 895
BamHI GGATCC 1 cut(s) 12
BanI GGYRCC 3 cut(s) 77, 308, 530
BarI GAAGNNNNNNTAC 2 cut(s) 439, 471
BbsI GAAGAC 2 cut(s) 128, 196
Bbv12I GWGCWC 5 cut(s) 75, 719, 895, 1170, 1224
BbvI GCAGC 5 cut(s) 40, 622, 905, 1034, 1201
BccI CCATC 8 cut(s) 89, 320, 356, 488, 542, 1253, 1257, 1261
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 990
BfaI CTAG 2 cut(s) 726, 1067
BglII AGATCT 2 cut(s) 697, 765
BisI GCNGC 8 cut(s) 21, 54, 181, 438, 636, 919, 1023, 1215
BlnI CCTAGG 1 cut(s) 725
BlsI GCNGC 8 cut(s) 22, 55, 182, 439, 637, 920, 1024, 1216
Bme1390I CCNGG 1 cut(s) 990
Bme18I GGWCC 2 cut(s) 986, 1231
BmeRI GACNNNNNGTC 1 cut(s) 251
BmgT120I GGNCC 2 cut(s) 986, 1231
BmiI GGNNCC 7 cut(s) 14, 79, 310, 322, 532, 1100, 1232
BmrFI CCNGG 1 cut(s) 990
BmrI ACTGGG 1 cut(s) 974
BmsI GCATC 4 cut(s) 33, 87, 580, 622
BmuI ACTGGG 1 cut(s) 974
BpiI GAAGAC 2 cut(s) 128, 196
BpmI CTGGAG 2 cut(s) 561, 624
Bpu10I CCTNAGC 1 cut(s) 25
BpuEI CTTGAG 1 cut(s) 678
BsaJI CCNNGG 3 cut(s) 36, 675, 725
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 581, 726, 1061
Bse118I RCCGGY 2 cut(s) 46, 56
Bse1I ACTGG 4 cut(s) 626, 963, 969, 1058
Bse3DI GCAATG 1 cut(s) 421
BseBI CCWGG 1 cut(s) 990
BseDI CCNNGG 3 cut(s) 36, 675, 725
BseGI GGATG 3 cut(s) 537, 1268, 1272
BseLI CCNNNNNNNGG 4 cut(s) 176, 581, 726, 1061
BseMI GCAATG 1 cut(s) 421
BseMII CTCAG 2 cut(s) 677, 1185
BseNI ACTGG 4 cut(s) 626, 963, 969, 1058
BseRI GAGGAG 2 cut(s) 561, 564
BseSI GKGCMC 1 cut(s) 895
BseXI GCAGC 5 cut(s) 40, 622, 905, 1034, 1201
BseYI CCCAGC 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 938
BshFI GGCC 2 cut(s) 146, 180
BshNI GGYRCC 3 cut(s) 77, 308, 530
BsiHKAI GWGCWC 5 cut(s) 75, 719, 895, 1170, 1224
BsiSI CCGG 2 cut(s) 47, 57
BslFI GGGAC 3 cut(s) 464, 635, 947
BslI CCNNNNNNNGG 4 cut(s) 176, 581, 726, 1061
BsmFI GGGAC 3 cut(s) 464, 635, 947
BsnI GGCC 2 cut(s) 146, 180
Bsp1286I GDGCHC 5 cut(s) 75, 719, 895, 1170, 1224
Bsp1407I TGTACA 1 cut(s) 709
Bsp143I GATC 6 cut(s) 12, 65, 228, 697, 765, 831
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 4 cut(s) 21, 181, 359, 437
BspANI GGCC 2 cut(s) 146, 180
BspCNI CTCAG 2 cut(s) 676, 1184
BspFNI CGCG 1 cut(s) 938
BspLI GGNNCC 7 cut(s) 14, 79, 310, 322, 532, 1100, 1232
BspPI GGATC 4 cut(s) 7, 20, 73, 826
BspQI GCTCTTC 1 cut(s) 1229
BspT107I GGYRCC 3 cut(s) 77, 308, 530
BsrDI GCAATG 1 cut(s) 421
BsrFI RCCGGY 2 cut(s) 46, 56
BsrGI TGTACA 1 cut(s) 709
BsrI ACTGG 4 cut(s) 626, 963, 969, 1058
BssAI RCCGGY 2 cut(s) 46, 56
BssECI CCNNGG 3 cut(s) 36, 675, 725
BssMI GATC 6 cut(s) 12, 65, 228, 697, 765, 831
BssT1I CCWWGG 3 cut(s) 36, 675, 725
Bst2UI CCWGG 1 cut(s) 990
Bst4CI ACNGT 3 cut(s) 948, 1041, 1081
Bst6I CTCTTC 2 cut(s) 727, 1229
BstAPI GCANNNNNTGC 2 cut(s) 824, 1153
BstAUI TGTACA 1 cut(s) 709
BstC8I GCNNGC 1 cut(s) 816
BstDEI CTNAG 4 cut(s) 25, 663, 1171, 1190
BstDSI CCRYGG 1 cut(s) 36
BstENI CCTNNNNNAGG 1 cut(s) 579
BstF5I GGATG 3 cut(s) 537, 1268, 1272
BstFNI CGCG 1 cut(s) 938
BstKTI GATC 6 cut(s) 15, 68, 231, 700, 768, 834
BstMBI GATC 6 cut(s) 12, 65, 228, 697, 765, 831
BstMWI GCNNNNNNNGC 5 cut(s) 26, 411, 495, 824, 1153
BstNI CCWGG 1 cut(s) 990
BstSCI CCNGG 1 cut(s) 988
BstSLI GKGCMC 1 cut(s) 895
BstUI CGCG 1 cut(s) 938
BstV1I GCAGC 5 cut(s) 40, 622, 905, 1034, 1201
BstV2I GAAGAC 2 cut(s) 128, 196
BstX2I RGATCY 3 cut(s) 12, 697, 765
BstYI RGATCY 3 cut(s) 12, 697, 765
BsuRI GGCC 2 cut(s) 146, 180
BtgI CCRYGG 1 cut(s) 36
BtgZI GCGATG 1 cut(s) 953
BtsCI GGATG 3 cut(s) 537, 1268, 1272
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 5 cut(s) 75, 281, 498, 962, 1170
Cac8I GCNNGC 1 cut(s) 816
CaiI CAGNNNCTG 2 cut(s) 1178, 1217
Cfr10I RCCGGY 2 cut(s) 46, 56
Cfr13I GGNCC 2 cut(s) 986, 1231
CseI GACGC 1 cut(s) 944
Csp6I GTAC 5 cut(s) 78, 154, 556, 710, 1271
CviAII CATG 5 cut(s) 37, 121, 142, 841, 922
CviQI GTAC 5 cut(s) 78, 154, 556, 710, 1271
DdeI CTNAG 4 cut(s) 25, 663, 1171, 1190
DpnI GATC 6 cut(s) 14, 67, 230, 699, 767, 833
DpnII GATC 6 cut(s) 12, 65, 228, 697, 765, 831
DraI TTTAAA 1 cut(s) 615
DraIII CACNNNGTG 1 cut(s) 123
DriI GACNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 2 cut(s) 727, 1229
Eam1105I GACNNNNNGTC 1 cut(s) 251
EarI CTCTTC 2 cut(s) 727, 1229
EciI GGCGGA 1 cut(s) 348
Eco130I CCWWGG 3 cut(s) 36, 675, 725
Eco47I GGWCC 2 cut(s) 986, 1231
Eco57I CTGAAG 4 cut(s) 257, 615, 818, 1127
EcoNI CCTNNNNNAGG 1 cut(s) 579
EcoRII CCWGG 1 cut(s) 988
EcoT14I CCWWGG 3 cut(s) 36, 675, 725
ErhI CCWWGG 3 cut(s) 36, 675, 725
FaeI CATG 5 cut(s) 40, 124, 145, 844, 925
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FaqI GGGAC 3 cut(s) 464, 635, 947
FatI CATG 5 cut(s) 36, 120, 141, 840, 921
FauNDI CATATG 1 cut(s) 550
Fnu4HI GCNGC 8 cut(s) 21, 54, 181, 438, 636, 919, 1023, 1215
FokI GGATG 3 cut(s) 524, 1275, 1279
Fsp4HI GCNGC 8 cut(s) 21, 54, 181, 438, 636, 919, 1023, 1215
FspBI CTAG 2 cut(s) 726, 1067
GluI GCNGC 8 cut(s) 21, 54, 181, 438, 636, 919, 1023, 1215
GsaI CCCAGC 1 cut(s) 241
GsuI CTGGAG 2 cut(s) 561, 624
HaeIII GGCC 2 cut(s) 146, 180
HapII CCGG 2 cut(s) 47, 57
HgaI GACGC 1 cut(s) 944
Hin1II CATG 5 cut(s) 40, 124, 145, 844, 925
HincII GTYRAC 2 cut(s) 160, 295
HindII GTYRAC 2 cut(s) 160, 295
HinfI GANTC 6 cut(s) 32, 473, 691, 925, 1071, 1208
HpaI GTTAAC 2 cut(s) 160, 295
HpaII CCGG 2 cut(s) 47, 57
HphI GGTGA 2 cut(s) 109, 482
Hpy166II GTNNAC 6 cut(s) 160, 295, 381, 556, 893, 1234
Hpy188I TCNGA 7 cut(s) 12, 245, 478, 666, 851, 1127, 1295
Hpy188III TCNNGA 7 cut(s) 190, 578, 641, 695, 1012, 1075, 1173
Hpy8I GTNNAC 6 cut(s) 160, 295, 381, 556, 893, 1234
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 5 cut(s) 575, 632, 1112, 1151, 1197
HpyCH4III ACNGT 3 cut(s) 948, 1041, 1081
HpyCH4IV ACGT 2 cut(s) 156, 297
HpyCH4V TGCA 5 cut(s) 107, 761, 818, 893, 921
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 411, 495, 824, 1153
HpyF3I CTNAG 4 cut(s) 25, 663, 1171, 1190
HpySE526I ACGT 2 cut(s) 156, 297
Hsp92II CATG 5 cut(s) 40, 124, 145, 844, 925
KpnI GGTACC 1 cut(s) 81
KspAI GTTAAC 2 cut(s) 160, 295
Kzo9I GATC 6 cut(s) 12, 65, 228, 697, 765, 831
LguI GCTCTTC 1 cut(s) 1229
LmnI GCTCC 5 cut(s) 326, 574, 643, 851, 1175
Lsp1109I GCAGC 5 cut(s) 40, 622, 905, 1034, 1201
LweI GCATC 4 cut(s) 33, 87, 580, 622
MaeI CTAG 2 cut(s) 726, 1067
MaeII ACGT 2 cut(s) 156, 297
MaeIII GTNAC 4 cut(s) 253, 470, 1075, 1134
MalI GATC 6 cut(s) 14, 67, 230, 699, 767, 833
MboI GATC 6 cut(s) 12, 65, 228, 697, 765, 831
MfeI CAATTG 2 cut(s) 102, 819
MflI RGATCY 3 cut(s) 12, 697, 765
MhlI GDGCHC 5 cut(s) 75, 719, 895, 1170, 1224
MluCI AATT 6 cut(s) 102, 393, 667, 804, 819, 1032
MlyI GAGTC 2 cut(s) 467, 685
MmeI TCCRAC 1 cut(s) 483
MseI TTAA 5 cut(s) 159, 294, 614, 981, 1281
MslI CAYNNNNRTG 1 cut(s) 41
MspA1I CMGCKG 2 cut(s) 918, 1214
MspI CCGG 2 cut(s) 47, 57
MspR9I CCNGG 1 cut(s) 990
MunI CAATTG 2 cut(s) 102, 819
MvaI CCWGG 1 cut(s) 990
MvnI CGCG 1 cut(s) 938
MwoI GCNNNNNNNGC 5 cut(s) 26, 411, 495, 824, 1153
NcoI CCATGG 1 cut(s) 36
NdeI CATATG 1 cut(s) 550
NdeII GATC 6 cut(s) 12, 65, 228, 697, 765, 831
NlaIII CATG 5 cut(s) 40, 124, 145, 844, 925
NlaIV GGNNCC 7 cut(s) 14, 79, 310, 322, 532, 1100, 1232
NmuCI GTSAC 3 cut(s) 253, 470, 1075
PciSI GCTCTTC 1 cut(s) 1229
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 336
PfeI GAWTC 4 cut(s) 32, 925, 1071, 1208
PkrI GCNGC 8 cut(s) 22, 55, 182, 439, 637, 920, 1024, 1216
PleI GAGTC 2 cut(s) 467, 685
PpsI GAGTC 2 cut(s) 467, 685
PsiI TTATAA 1 cut(s) 1031
Psp6I CCWGG 1 cut(s) 988
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 1 cut(s) 988
PspN4I GGNNCC 7 cut(s) 14, 79, 310, 322, 532, 1100, 1232
PspPI GGNCC 2 cut(s) 986, 1231
PstNI CAGNNNCTG 2 cut(s) 1178, 1217
PsuI RGATCY 3 cut(s) 12, 697, 765
PvuII CAGCTG 2 cut(s) 918, 1214
RsaI GTAC 5 cut(s) 79, 155, 557, 711, 1272
RsaNI GTAC 5 cut(s) 78, 154, 556, 710, 1271
RseI CAYNNNNRTG 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 1229
SaqAI TTAA 5 cut(s) 159, 294, 614, 981, 1281
SatI GCNGC 8 cut(s) 21, 54, 181, 438, 636, 919, 1023, 1215
Sau3AI GATC 6 cut(s) 12, 65, 228, 697, 765, 831
Sau96I GGNCC 2 cut(s) 986, 1231
SchI GAGTC 2 cut(s) 467, 685
ScrFI CCNGG 1 cut(s) 990
SduI GDGCHC 5 cut(s) 75, 719, 895, 1170, 1224
SfaNI GCATC 4 cut(s) 33, 87, 580, 622
SinI GGWCC 2 cut(s) 986, 1231
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 693
SmoI CTYRAG 1 cut(s) 693
Sse9I AATT 6 cut(s) 102, 393, 667, 804, 819, 1032
SsiI CCGC 4 cut(s) 21, 181, 359, 437
SspMI CTAG 2 cut(s) 726, 1067
StyD4I CCNGG 1 cut(s) 988
StyI CCWWGG 3 cut(s) 36, 675, 725
TaaI ACNGT 3 cut(s) 948, 1041, 1081
TaiI ACGT 2 cut(s) 159, 300
TaqI TCGA 1 cut(s) 830
TasI AATT 6 cut(s) 102, 393, 667, 804, 819, 1032
TatI WGTACW 1 cut(s) 709
TauI GCSGC 3 cut(s) 23, 183, 440
TfiI GAWTC 4 cut(s) 32, 925, 1071, 1208
Tru1I TTAA 5 cut(s) 159, 294, 614, 981, 1281
Tru9I TTAA 5 cut(s) 159, 294, 614, 981, 1281
TscAI CASTG 5 cut(s) 75, 281, 505, 969, 1170
TseFI GTSAC 3 cut(s) 253, 470, 1075
TseI GCWGC 5 cut(s) 53, 635, 918, 1022, 1214
Tsp45I GTSAC 3 cut(s) 253, 470, 1075
TspDTI ATGAA 2 cut(s) 274, 325
TspGWI ACGGA 1 cut(s) 999
TspRI CASTG 5 cut(s) 75, 281, 505, 969, 1170
VneI GTGCAC 1 cut(s) 891
VpaK11BI GGWCC 2 cut(s) 986, 1231
XagI CCTNNNNNAGG 1 cut(s) 579
XapI RAATTY 1 cut(s) 667
XcmI CCANNNNNNNNNTGG 2 cut(s) 127, 1252
XmaJI CCTAGG 1 cut(s) 725
XspI CTAG 2 cut(s) 726, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.