MD04G1178500.v1.1

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003304
Physical Location & Seq
Reverse (-)
26933280 .. 26937453
4174 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1178500.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGGAGAGGATTTTGGCGGCGGTGGAAGTGGTGAATCAAGCGGGAGGGGAATCGTTACTTGGGACAATCAAGATCGCCGTGCTTCCCATAGCAAAGGTTTTTACAGTCTGCTCCTTGGGGCTTCTAATGGCTTCCAAGTATGTGAACATCTTCCCAGCCAGTGGAAGGAAATTGTTGAATGGGTTGGTCTTCTCGCTCTTGCTTCCATGTTTGATATTCTCTCAGCTTGGACAAGCCATCACCCTACATAAAATGCTTGAGTGGTGGTTTATTCCCGTGAATGTCGTGATTGGTAGCACTACAGGCTCCATAATAGGTTATATTGTTGCATCCCTTGTCCACCCACCATACCCTTTCTTCAAGTTTACAATTGTACAAATTGGAATAGGAAACATCGGCAATGTGCCACTGGTTCTAATTTCAGCTTTATGTAGAGACAAATCGAACCCTTTCGGTGATTCAACCACATGTAAAACAGATGGAACTGCCTATATTTCATTTGGCCAGTGGGTTGGTGCAATCATTCTATACACGTATGTATTTCAAATGCTGTCCCCCCCTCCAGAAGGTACCTTTGACGTTGAGGAAAAAGAACTCCCAATCAAAAGCCCTCGAAATGGCACGACACCTGATCAAGTTCCATTGCTTACACCTGACGAAAATGAAGAAGAGACAGCACGTAAAGAAGAGGTTGCAGAAACTGAGTCGAATGCTTCAAATAAGCCTAAGATTACAAAGTTCTTCTTATTCATCTATGAGAAGTTGAAGCTCAAACAAGTTCTTCAACCACCTATAATAGCTTCTATCCTGGCCATGGTACTTGGAACCATACCATTTTTAAAGAAATTGATCTTTACATCTGATGGTCCACTTTTCTTCTTCACCGACAGCTGCATTATCCTTGGGGAAGCGATGATTCCATGCATTCTGTTGGCGTTAGGTGGCAACCTTGTTGATGGTCCGGGAAGTTCCAAACTTGGTCTACGAACAACTGCTGCAATTATATTCGCACGATTAGTCTTGGTGCCCCCTGTAGGACTTGGCGTTGTAATGTTAGCTGATAAGCTCGGCTTCCTCCCTCCCAATGACCAGATGTTCCGGTTTGTCCTGCTGCTCCAGCACACAATGCCTACATCTGTCCTCGCTGGTGCTGTTGCAAATTTAAGAGGCTGTGGAAGAGAGGCAGCTGCCGTCCTGTTCTGGGTGCACATATTCGCCATCTTCTCCATGGCCGGTTGGATCGTCTTGTACCTCAACATACTCTTCTGA

Protein Analysis

423

Amino Acids

45.85

Weight (kDa)

7.55

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 27 - 414 1.3e-94 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 569
AccB1I GGYRCC 2 cut(s) 569, 1024
AccB7I CCANNNNNTGG 1 cut(s) 161
AccI GTMKAC 1 cut(s) 982
AciI CCGC 3 cut(s) 17, 20, 41
AclWI GGATC 1 cut(s) 1247
AcoI YGGCCR 3 cut(s) 502, 810, 1230
AcsI RAATTY 1 cut(s) 1159
AfaI GTAC 4 cut(s) 375, 571, 819, 1250
AfiI CCNNNNNNNGG 7 cut(s) 161, 165, 566, 617, 814, 1034, 1201
AflIII ACRYGT 2 cut(s) 467, 531
AgsI TTSAA 7 cut(s) 178, 361, 462, 545, 717, 766, 785
AjnI CCWGG 1 cut(s) 807
AjuI GAANNNNNNNTTGG 2 cut(s) 42, 74
AluBI AGCT 8 cut(s) 226, 425, 769, 800, 891, 1058, 1066, 1187
AluI AGCT 8 cut(s) 226, 425, 769, 800, 891, 1058, 1066, 1187
Alw21I GWGCWC 1 cut(s) 1209
Alw26I GTCTC 2 cut(s) 429, 665
Alw44I GTGCAC 1 cut(s) 1205
AlwI GGATC 1 cut(s) 1247
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 3 cut(s) 502, 810, 1230
ApaLI GTGCAC 1 cut(s) 1205
ApeKI GCWGC 5 cut(s) 891, 995, 1111, 1184, 1187
ApoI RAATTY 1 cut(s) 1159
Asp700I GAANNNNTTC 2 cut(s) 149, 449
Asp718I GGTACC 1 cut(s) 569
AspS9I GGNCC 2 cut(s) 866, 959
AsuC2I CCSGG 1 cut(s) 963
AsuHPI GGTGA 4 cut(s) 43, 232, 467, 874
AvaII GGWCC 2 cut(s) 866, 959
BaeGI GKGCMC 2 cut(s) 1029, 1209
BalI TGGCCA 2 cut(s) 504, 812
BanI GGYRCC 2 cut(s) 569, 1024
BbsI GAAGAC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 1209
BbvI GCAGC 5 cut(s) 878, 982, 1098, 1174, 1196
BccI CCATC 5 cut(s) 245, 473, 857, 950, 1226
BceAI ACGGC 2 cut(s) 62, 1175
BciT130I CCWGG 1 cut(s) 809
BclI TGATCA 1 cut(s) 631
BcnI CCSGG 1 cut(s) 963
BcoDI GTCTC 2 cut(s) 429, 665
BfmI CTRYAG 2 cut(s) 300, 1032
BisI GCNGC 6 cut(s) 18, 892, 996, 1112, 1185, 1188
BlsI GCNGC 6 cut(s) 19, 893, 997, 1113, 1186, 1189
Bme1390I CCNGG 2 cut(s) 809, 963
Bme18I GGWCC 2 cut(s) 866, 959
BmgT120I GGNCC 2 cut(s) 866, 959
BmiI GGNNCC 4 cut(s) 307, 571, 826, 1026
BmrFI CCNGG 2 cut(s) 809, 963
BmsI GCATC 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 181
BpmI CTGGAG 2 cut(s) 546, 1100
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 963
BsaAI YACGTR 2 cut(s) 534, 680
BsaJI CCNNGG 4 cut(s) 114, 813, 901, 1227
BsaWI WCCGGW 1 cut(s) 1098
Bsc4I CCNNNNNNNGG 7 cut(s) 161, 165, 566, 617, 814, 1034, 1201
Bse118I RCCGGY 1 cut(s) 1232
Bse1I ACTGG 3 cut(s) 159, 414, 505
Bse3DI GCAATG 2 cut(s) 406, 641
BseBI CCWGG 1 cut(s) 809
BseDI CCNNGG 4 cut(s) 114, 813, 901, 1227
BseGI GGATG 1 cut(s) 329
BseLI CCNNNNNNNGG 7 cut(s) 161, 165, 566, 617, 814, 1034, 1201
BseMI GCAATG 2 cut(s) 406, 641
BseMII CTCAG 2 cut(s) 236, 693
BseNI ACTGG 3 cut(s) 159, 414, 505
BseSI GKGCMC 2 cut(s) 1029, 1209
BseXI GCAGC 5 cut(s) 878, 982, 1098, 1174, 1196
BseYI CCCAGC 1 cut(s) 154
BshFI GGCC 3 cut(s) 504, 812, 1232
BshNI GGYRCC 2 cut(s) 569, 1024
BsiHKAI GWGCWC 1 cut(s) 1209
BsiSI CCGG 3 cut(s) 962, 1099, 1233
BslFI GGGAC 2 cut(s) 76, 538
BslI CCNNNNNNNGG 7 cut(s) 161, 165, 566, 617, 814, 1034, 1201
BsmAI GTCTC 2 cut(s) 429, 665
BsmFI GGGAC 2 cut(s) 76, 538
BsmI GAATGC 2 cut(s) 715, 924
BsnI GGCC 3 cut(s) 504, 812, 1232
Bsp1286I GDGCHC 2 cut(s) 1029, 1209
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 4 cut(s) 72, 631, 849, 1239
Bsp19I CCATGG 2 cut(s) 813, 1227
BspACI CCGC 3 cut(s) 17, 20, 41
BspANI GGCC 3 cut(s) 504, 812, 1232
BspCNI CTCAG 2 cut(s) 235, 694
BspLI GGNNCC 4 cut(s) 307, 571, 826, 1026
BspPI GGATC 1 cut(s) 1247
BspT107I GGYRCC 2 cut(s) 569, 1024
BsrDI GCAATG 2 cut(s) 406, 641
BsrFI RCCGGY 1 cut(s) 1232
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 3 cut(s) 159, 414, 505
BssAI RCCGGY 1 cut(s) 1232
BssECI CCNNGG 4 cut(s) 114, 813, 901, 1227
BssMI GATC 4 cut(s) 72, 631, 849, 1239
BssT1I CCWWGG 4 cut(s) 114, 813, 901, 1227
Bst2UI CCWGG 1 cut(s) 809
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 3 cut(s) 663, 681, 1171
BstAPI GCANNNNNTGC 1 cut(s) 1126
BstAUI TGTACA 1 cut(s) 373
BstBAI YACGTR 2 cut(s) 534, 680
BstDEI CTNAG 3 cut(s) 222, 702, 726
BstDSI CCRYGG 2 cut(s) 813, 1227
BstENI CCTNNNNNAGG 1 cut(s) 564
BstF5I GGATG 1 cut(s) 329
BstKTI GATC 4 cut(s) 75, 634, 852, 1242
BstMAI GTCTC 2 cut(s) 429, 665
BstMBI GATC 4 cut(s) 72, 631, 849, 1239
BstMWI GCNNNNNNNGC 3 cut(s) 303, 1117, 1126
BstNI CCWGG 1 cut(s) 809
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 807, 961
BstSFI CTRYAG 2 cut(s) 300, 1032
BstSLI GKGCMC 2 cut(s) 1029, 1209
BstV1I GCAGC 5 cut(s) 878, 982, 1098, 1174, 1196
BstV2I GAAGAC 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 512
BsuRI GGCC 3 cut(s) 504, 812, 1232
BtgI CCRYGG 2 cut(s) 813, 1227
BtgZI GCGATG 1 cut(s) 926
BtsCI GGATG 1 cut(s) 329
BtsIMutI CAGTG 3 cut(s) 166, 407, 512
CaiI CAGNNNCTG 1 cut(s) 701
Cfr10I RCCGGY 1 cut(s) 1232
Cfr13I GGNCC 2 cut(s) 866, 959
Csp6I GTAC 4 cut(s) 374, 570, 818, 1249
CviAII CATG 5 cut(s) 207, 468, 814, 921, 1228
CviQI GTAC 4 cut(s) 374, 570, 818, 1249
DdeI CTNAG 3 cut(s) 222, 702, 726
DpnI GATC 4 cut(s) 74, 633, 851, 1241
DpnII GATC 4 cut(s) 72, 631, 849, 1239
DraI TTTAAA 1 cut(s) 840
EaeI YGGCCR 3 cut(s) 502, 810, 1230
Eam1104I CTCTTC 3 cut(s) 663, 681, 1171
EarI CTCTTC 3 cut(s) 663, 681, 1171
Eco130I CCWWGG 4 cut(s) 114, 813, 901, 1227
Eco47I GGWCC 2 cut(s) 866, 959
EcoNI CCTNNNNNAGG 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 807
EcoT14I CCWWGG 4 cut(s) 114, 813, 901, 1227
EcoT22I ATGCAT 1 cut(s) 926
ErhI CCWWGG 4 cut(s) 114, 813, 901, 1227
FaeI CATG 5 cut(s) 210, 471, 817, 924, 1231
FalI AAGNNNNNCTT 4 cut(s) 728, 760, 1055, 1087
FaqI GGGAC 2 cut(s) 76, 538
FatI CATG 5 cut(s) 206, 467, 813, 920, 1227
FauI CCCGC 1 cut(s) 34
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 1 cut(s) 982
Fnu4HI GCNGC 6 cut(s) 18, 892, 996, 1112, 1185, 1188
FokI GGATG 1 cut(s) 316
Fsp4HI GCNGC 6 cut(s) 18, 892, 996, 1112, 1185, 1188
GluI GCNGC 6 cut(s) 18, 892, 996, 1112, 1185, 1188
GsaI CCCAGC 1 cut(s) 158
GsuI CTGGAG 2 cut(s) 546, 1100
HaeIII GGCC 3 cut(s) 504, 812, 1232
HapII CCGG 3 cut(s) 962, 1099, 1233
Hin1II CATG 5 cut(s) 210, 471, 817, 924, 1231
HinfI GANTC 5 cut(s) 34, 50, 458, 704, 916
HpaII CCGG 3 cut(s) 962, 1099, 1233
HphI GGTGA 4 cut(s) 43, 232, 467, 874
Hpy166II GTNNAC 6 cut(s) 145, 340, 366, 869, 983, 1207
Hpy188I TCNGA 2 cut(s) 862, 1268
Hpy188III TCNNGA 3 cut(s) 70, 286, 563
Hpy8I GTNNAC 6 cut(s) 145, 340, 366, 869, 983, 1207
HpyAV CCTTC 2 cut(s) 159, 560
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 3 cut(s) 533, 579, 679
HpyCH4V TGCA 8 cut(s) 329, 518, 695, 894, 924, 998, 1157, 1207
HpyF10VI GCNNNNNNNGC 3 cut(s) 303, 1117, 1126
HpyF3I CTNAG 3 cut(s) 222, 702, 726
HpySE526I ACGT 3 cut(s) 533, 579, 679
Hsp92II CATG 5 cut(s) 210, 471, 817, 924, 1231
KpnI GGTACC 1 cut(s) 573
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 4 cut(s) 72, 631, 849, 1239
LmnI GCTCC 3 cut(s) 116, 311, 1119
Lsp1109I GCAGC 5 cut(s) 878, 982, 1098, 1174, 1196
LweI GCATC 1 cut(s) 338
MaeII ACGT 3 cut(s) 533, 579, 679
MaeIII GTNAC 1 cut(s) 54
MalI GATC 4 cut(s) 74, 633, 851, 1241
MboI GATC 4 cut(s) 72, 631, 849, 1239
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 2 cut(s) 1029, 1209
MlsI TGGCCA 2 cut(s) 504, 812
MluCI AATT 7 cut(s) 170, 369, 378, 417, 845, 999, 1159
MluNI TGGCCA 2 cut(s) 504, 812
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 1 cut(s) 1217
Mox20I TGGCCA 2 cut(s) 504, 812
Mph1103I ATGCAT 1 cut(s) 926
MroXI GAANNNNTTC 2 cut(s) 149, 449
MscI TGGCCA 2 cut(s) 504, 812
MseI TTAA 2 cut(s) 839, 1163
Msp20I TGGCCA 2 cut(s) 504, 812
MspA1I CMGCKG 2 cut(s) 891, 1187
MspI CCGG 3 cut(s) 962, 1099, 1233
MspR9I CCNGG 2 cut(s) 809, 963
MunI CAATTG 1 cut(s) 369
Mva1269I GAATGC 2 cut(s) 715, 924
MvaI CCWGG 1 cut(s) 809
MwoI GCNNNNNNNGC 3 cut(s) 303, 1117, 1126
NciI CCSGG 1 cut(s) 963
NcoI CCATGG 2 cut(s) 813, 1227
NdeII GATC 4 cut(s) 72, 631, 849, 1239
NlaIII CATG 5 cut(s) 210, 471, 817, 924, 1231
NlaIV GGNNCC 4 cut(s) 307, 571, 826, 1026
NmeAIII GCCGAG 1 cut(s) 1047
NsiI ATGCAT 1 cut(s) 926
NspI RCATGY 1 cut(s) 471
PciI ACATGT 1 cut(s) 467
PctI GAATGC 2 cut(s) 715, 924
PdmI GAANNNNTTC 2 cut(s) 149, 449
PfeI GAWTC 4 cut(s) 34, 50, 458, 916
PflMI CCANNNNNTGG 1 cut(s) 161
PfoI TCCNGGA 1 cut(s) 961
PkrI GCNGC 6 cut(s) 19, 893, 997, 1113, 1186, 1189
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
Ppu21I YACGTR 2 cut(s) 534, 680
PscI ACATGT 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 807
PspFI CCCAGC 1 cut(s) 154
PspGI CCWGG 1 cut(s) 807
PspN4I GGNNCC 4 cut(s) 307, 571, 826, 1026
PspPI GGNCC 2 cut(s) 866, 959
PstNI CAGNNNCTG 1 cut(s) 701
PvuII CAGCTG 2 cut(s) 891, 1187
RsaI GTAC 4 cut(s) 375, 571, 819, 1250
RsaNI GTAC 4 cut(s) 374, 570, 818, 1249
SaqAI TTAA 2 cut(s) 839, 1163
SatI GCNGC 6 cut(s) 18, 892, 996, 1112, 1185, 1188
Sau3AI GATC 4 cut(s) 72, 631, 849, 1239
Sau96I GGNCC 2 cut(s) 866, 959
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 2 cut(s) 809, 963
SduI GDGCHC 2 cut(s) 1029, 1209
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 2 cut(s) 300, 1032
SinI GGWCC 2 cut(s) 866, 959
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 7 cut(s) 170, 369, 378, 417, 845, 999, 1159
SsiI CCGC 3 cut(s) 17, 20, 41
StyD4I CCNGG 2 cut(s) 807, 961
StyI CCWWGG 4 cut(s) 114, 813, 901, 1227
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 3 cut(s) 536, 582, 682
TaqI TCGA 3 cut(s) 443, 613, 707
TasI AATT 7 cut(s) 170, 369, 378, 417, 845, 999, 1159
TatI WGTACW 1 cut(s) 373
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 4 cut(s) 34, 50, 458, 916
Tru1I TTAA 2 cut(s) 839, 1163
Tru9I TTAA 2 cut(s) 839, 1163
TscAI CASTG 3 cut(s) 166, 414, 512
TseI GCWGC 5 cut(s) 891, 995, 1111, 1184, 1187
TspDTI ATGAA 3 cut(s) 486, 678, 739
TspRI CASTG 3 cut(s) 166, 414, 512
Van91I CCANNNNNTGG 1 cut(s) 161
VneI GTGCAC 1 cut(s) 1205
VpaK11BI GGWCC 2 cut(s) 866, 959
XagI CCTNNNNNAGG 1 cut(s) 564
XapI RAATTY 1 cut(s) 1159
XceI RCATGY 1 cut(s) 471
XcmI CCANNNNNNNNNTGG 1 cut(s) 1225
XmiI GTMKAC 1 cut(s) 982
XmnI GAANNNNTTC 2 cut(s) 149, 449
Zsp2I ATGCAT 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.