Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0003304

Physical Location & Seq

Genomic Coordinates Forward (+)

5544940 .. 5549694

Gene Structure 4755 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g09330.t6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGGAGAGCATTTTGACAATGGTGGAGATGGGGAGTCAAGTAGGAAGCCAGTCGTTACTCAGTACAATCAAGATTGCAGTATTGCCAATAGCAAAGGTTTTTACGGTTTGTTCTTTGGGTCTTCTTATGGCTTCCAAGTATGTTAACATCTTGCCGGCTAATGGGAGGAAGCTCTTGAATGGGCTGGTTTTCTCGCTTTTGCTTCCGTGCTTGATATTCTCCCAGCTCGGACAAGCTATCACCTGGGAGAAAATGCTCGAGTGGTGGTTTATTCCTGCGAATGTAGTTCTTGGTAGTATATCAGGCTCAATTATAGGTTATATAACCGCATCCATTGTTCGTCCACCATACCCATACTTCAAGTTCACAATTGTACAGATTGGAATAGGAAACATTGGGAATGTGCCACTTGTTCTGATTGCCGCTCTATGTAGAGACACATCCAACCCTTTCGGTGACACATGTAGTACAAATGGGACTGCCTATATTTCGTTTGGCCAGTGGGTTGGTGCAATCATCCTTTACACTTACGTATTTCATATGTTGGCCCCTCCTCCAGAGGGTACCTTTGACATTGATGAGGAAACTCTCCCAATCAAGAGCCCTAGTGATGGAGACACGACTGGACAAGTTCCTTTGCTTACACATGACGAACAAGAAGAAGAGGAAGAGCAAAAACATGAAGAAGTTGTAGAAACCAACACAAATTCTTCAAAGAAATCTAAGATTATAGAGATCTTGATTTTTTTGTATGAGAAGTTGAACCTCAAACAACTTCTTCATCCCCCTATAATAGCTTCTGTCCTAGCCATGTTCCTTGGCGCAATACCATTCTTAAAAAAACAGATCTTTGAATCTGGCGGTCCACTTTTCTTCTTCACTGACAGCTGCACTATCCTTGGGGATGCCATGATTCCATGCATTCTTTTGGCATTAGGCGGCAACCTTATCAATGGCCCAGGGAGTTCCAAACTTGGTTTTCGAACAACAGCCGCTATTATTTTTGCACGGTTGGTCTTGGTCCCCCCGGCAGGACTTGGGATTGTAACGTTGGCCGATAAGCTTGGTTTCCTTCCTGATGGTGATAAGATGTTTCGTTTTGTCCTACTGCTCCAGCACACAATGCCAACATCTGTTCTTGCTGGTGCTGTTGCAAATTTAAGAGGCTGTGGAAGGGAGGCCGCCGCTGTCTTGTTCTGGGTTCATATATTTGCGGTGTTTTCCATGGCTGGGTGGATTGTTCTTTACCTCAACATACTATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006810 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0012505 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0031984 GO:0042175 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mem_trans

Protein Analysis

422

Amino Acids

45.73

Weight (kDa)

5.58

Isoelectric Point (pI)

36.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mem_trans	PF03547	27 - 413	4.1e-95	Membrane transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003304)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01990
fragaria_vesca	FvH4_6g09330 FvH4_6g09330 FvH4_6g09330 FvH4_6g09330 FvH4_6g09330 FvH4_6g09330 FvH4_6g09330 FvH4_6g09330 FvH4_6g09330 FvH4_6g09330
malus_domestica	MD04G1178500.v1.1 MD12G1194000.v1.1
prunus_persica	Prupe.6G298500_v2.0.a1 Prupe.6G298500_v2.0.a1 Prupe.6G298500_v2.0.a1
pyrus_communis	pycom04g15750 pycom12g18020
rosa_chinensis	RchiOBHm_Chr3g0459731 RchiOBHm_Chr4g0399931
rosa_laevigata	RLG00000021505 RLG00000025062 RLG00000028723
rosa_multiflora	Rmu_sc0000240.1_g000034
rosa_roxburghii	Rroxscaffold_3G00242630 Rroxscaffold_6G00420020
rosa_rugosa	Rorug03G0037900 Rorug03G0037900 Rorug03G0038000.1 Rorug03G0038100
rosa_samantha	Rh1AG210400 Rh3AG096400 Rh3BG099100 Rh3CG101000 Rh3DG100400
rosa_wichuraiana	Rw4G008050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	563
AccB1I	GGYRCC	1 cut(s)	563
AccBSI	CCGCTC	1 cut(s)	425
AciI	CCGC	8 cut(s)	327, 423, 861, 939, 993, 1182, 1185, 1214
AclI	AACGTT	1 cut(s)	1049
AcoI	YGGCCR	2 cut(s)	496, 1053
AcsI	RAATTY	2 cut(s)	706, 1156
AfaI	GTAC	4 cut(s)	64, 375, 469, 565
AfiI	CCNNNNNNNGG	5 cut(s)	161, 560, 611, 1031, 1230
AflIII	ACRYGT	1 cut(s)	461
AgsI	TTSAA	5 cut(s)	178, 361, 714, 763, 854
AjnI	CCWGG	2 cut(s)	242, 958
AluBI	AGCT	6 cut(s)	172, 226, 236, 797, 888, 1063
AluI	AGCT	6 cut(s)	172, 226, 236, 797, 888, 1063
Alw26I	GTCTC	2 cut(s)	429, 609
Ama87I	CYCGRG	1 cut(s)	257
AoxI	GGCC	5 cut(s)	496, 546, 955, 1053, 1179
ApeKI	GCWGC	1 cut(s)	888
ApoI	RAATTY	2 cut(s)	706, 1156
Asp718I	GGTACC	1 cut(s)	563
AspLEI	GCGC	1 cut(s)	824
AspS9I	GGNCC	4 cut(s)	547, 863, 956, 1021
AsuC2I	CCSGG	1 cut(s)	1028
AsuHPI	GGTGA	3 cut(s)	232, 467, 1094
AsuII	TTCGAA	1 cut(s)	982
AvaI	CYCGRG	1 cut(s)	257
AvaII	GGWCC	2 cut(s)	863, 1021
BalI	TGGCCA	1 cut(s)	498
BanI	GGYRCC	1 cut(s)	563
BanII	GRGCYC	1 cut(s)	605
BbsI	GAAGAC	1 cut(s)	113
BbvI	GCAGC	1 cut(s)	875
BccI	CCATC	3 cut(s)	22, 605, 1073
BciT130I	CCWGG	2 cut(s)	244, 960
BcnI	CCSGG	1 cut(s)	1028
BcoDI	GTCTC	2 cut(s)	429, 609
BfaI	CTAG	2 cut(s)	606, 806
BglII	AGATCT	2 cut(s)	735, 846
BisI	GCNGC	6 cut(s)	423, 889, 940, 993, 1182, 1185
BlsI	GCNGC	6 cut(s)	424, 890, 941, 994, 1183, 1186
Bme1390I	CCNGG	3 cut(s)	244, 960, 1028
Bme18I	GGWCC	2 cut(s)	863, 1021
BmeT110I	CYCGRG	1 cut(s)	257
BmgT120I	GGNCC	4 cut(s)	547, 863, 956, 1021
BmiI	GGNNCC	3 cut(s)	549, 565, 1023
BmrFI	CCNGG	3 cut(s)	244, 960, 1028
BmsI	GCATC	2 cut(s)	338, 895
BpiI	GAAGAC	1 cut(s)	113
BpmI	CTGGAG	2 cut(s)	540, 1097
Bpu14I	TTCGAA	1 cut(s)	982
BpuMI	CCSGG	1 cut(s)	1028
BsaAI	YACGTR	1 cut(s)	532
BsaJI	CCNNGG	7 cut(s)	243, 817, 898, 958, 959, 1026, 1224
Bsc4I	CCNNNNNNNGG	5 cut(s)	161, 560, 611, 1031, 1230
Bse118I	RCCGGY	1 cut(s)	154
Bse1I	ACTGG	3 cut(s)	49, 499, 628
BseBI	CCWGG	2 cut(s)	244, 960
BseDI	CCNNGG	7 cut(s)	243, 817, 898, 958, 959, 1026, 1224
BseGI	GGATG	5 cut(s)	329, 440, 516, 781, 910
BseLI	CCNNNNNNNGG	5 cut(s)	161, 560, 611, 1031, 1230
BseMII	CTCAG	1 cut(s)	73
BseNI	ACTGG	3 cut(s)	49, 499, 628
BseRI	GAGGAG	1 cut(s)	543
BseXI	GCAGC	1 cut(s)	875
BseYI	CCCAGC	2 cut(s)	222, 1229
BsgI	GTGCAG	1 cut(s)	874
BshFI	GGCC	5 cut(s)	498, 548, 957, 1055, 1181
BshNI	GGYRCC	1 cut(s)	563
BsiHKCI	CYCGRG	1 cut(s)	257
BsiSI	CCGG	2 cut(s)	155, 1028
BslFI	GGGAC	2 cut(s)	490, 1007
BslI	CCNNNNNNNGG	5 cut(s)	161, 560, 611, 1031, 1230
BsmAI	GTCTC	2 cut(s)	429, 609
BsmFI	GGGAC	2 cut(s)	490, 1007
BsmI	GAATGC	1 cut(s)	921
BsnI	GGCC	5 cut(s)	498, 548, 957, 1055, 1181
BsoBI	CYCGRG	1 cut(s)	257
Bsp119I	TTCGAA	1 cut(s)	982
Bsp1286I	GDGCHC	1 cut(s)	605
Bsp1407I	TGTACA	1 cut(s)	373
Bsp143I	GATC	2 cut(s)	735, 846
Bsp19I	CCATGG	1 cut(s)	1224
BspACI	CCGC	8 cut(s)	327, 423, 861, 939, 993, 1182, 1185, 1214
BspANI	GGCC	5 cut(s)	498, 548, 957, 1055, 1181
BspCNI	CTCAG	1 cut(s)	72
BspLI	GGNNCC	3 cut(s)	549, 565, 1023
BspQI	GCTCTTC	1 cut(s)	663
BspT104I	TTCGAA	1 cut(s)	982
BspT107I	GGYRCC	1 cut(s)	563
BsrBI	CCGCTC	1 cut(s)	425
BsrFI	RCCGGY	1 cut(s)	154
BsrGI	TGTACA	1 cut(s)	373
BsrI	ACTGG	3 cut(s)	49, 499, 628
BssAI	RCCGGY	1 cut(s)	154
BssECI	CCNNGG	7 cut(s)	243, 817, 898, 958, 959, 1026, 1224
BssMI	GATC	2 cut(s)	735, 846
BssT1I	CCWWGG	3 cut(s)	817, 898, 1224
Bst2UI	CCWGG	2 cut(s)	244, 960
Bst4CI	ACNGT	2 cut(s)	106, 1011
Bst6I	CTCTTC	2 cut(s)	657, 663
BstAPI	GCANNNNNTGC	1 cut(s)	1123
BstAUI	TGTACA	1 cut(s)	373
BstBAI	YACGTR	1 cut(s)	532
BstBI	TTCGAA	1 cut(s)	982
BstC8I	GCNNGC	1 cut(s)	156
BstDEI	CTNAG	2 cut(s)	59, 723
BstDSI	CCRYGG	1 cut(s)	1224
BstF5I	GGATG	5 cut(s)	329, 440, 516, 781, 910
BstHHI	GCGC	1 cut(s)	824
BstKTI	GATC	2 cut(s)	738, 849
BstMAI	GTCTC	2 cut(s)	429, 609
BstMBI	GATC	2 cut(s)	735, 846
BstMWI	GCNNNNNNNGC	1 cut(s)	1123
BstNI	CCWGG	2 cut(s)	244, 960
BstNSI	RCATGY	1 cut(s)	465
BstSCI	CCNGG	3 cut(s)	242, 958, 1026
BstSNI	TACGTA	1 cut(s)	532
BstV1I	GCAGC	1 cut(s)	875
BstV2I	GAAGAC	1 cut(s)	113
BstX2I	RGATCY	2 cut(s)	735, 846
BstXI	CCANNNNNNTGG	1 cut(s)	506
BstYI	RGATCY	2 cut(s)	735, 846
BsuRI	GGCC	5 cut(s)	498, 548, 957, 1055, 1181
BtgI	CCRYGG	1 cut(s)	1224
BtsCI	GGATG	5 cut(s)	329, 440, 516, 781, 910
BtsIMutI	CAGTG	2 cut(s)	506, 879
Cac8I	GCNNGC	1 cut(s)	156
CfoI	GCGC	1 cut(s)	824
Cfr10I	RCCGGY	1 cut(s)	154
Cfr13I	GGNCC	4 cut(s)	547, 863, 956, 1021
Csp6I	GTAC	4 cut(s)	63, 374, 468, 564
CviAII	CATG	7 cut(s)	462, 647, 680, 811, 910, 918, 1225
CviQI	GTAC	4 cut(s)	63, 374, 468, 564
DdeI	CTNAG	2 cut(s)	59, 723
DpnI	GATC	2 cut(s)	737, 848
DpnII	GATC	2 cut(s)	735, 846
EaeI	YGGCCR	2 cut(s)	496, 1053
Eam1104I	CTCTTC	2 cut(s)	657, 663
EarI	CTCTTC	2 cut(s)	657, 663
Eco105I	TACGTA	1 cut(s)	532
Eco130I	CCWWGG	3 cut(s)	817, 898, 1224
Eco24I	GRGCYC	1 cut(s)	605
Eco47I	GGWCC	2 cut(s)	863, 1021
Eco88I	CYCGRG	1 cut(s)	257
EcoRII	CCWGG	2 cut(s)	242, 958
EcoT14I	CCWWGG	3 cut(s)	817, 898, 1224
EcoT22I	ATGCAT	1 cut(s)	923
EcoT38I	GRGCYC	1 cut(s)	605
ErhI	CCWWGG	3 cut(s)	817, 898, 1224
FaeI	CATG	7 cut(s)	465, 650, 683, 814, 913, 921, 1228
FaqI	GGGAC	2 cut(s)	490, 1007
FatI	CATG	7 cut(s)	461, 646, 679, 810, 909, 917, 1224
FauNDI	CATATG	1 cut(s)	540
Fnu4HI	GCNGC	6 cut(s)	423, 889, 940, 993, 1182, 1185
FokI	GGATG	5 cut(s)	316, 427, 503, 768, 917
FriOI	GRGCYC	1 cut(s)	605
Fsp4HI	GCNGC	6 cut(s)	423, 889, 940, 993, 1182, 1185
FspBI	CTAG	2 cut(s)	606, 806
GlaI	GCGC	1 cut(s)	823
GluI	GCNGC	6 cut(s)	423, 889, 940, 993, 1182, 1185
GsaI	CCCAGC	2 cut(s)	226, 1233
GsuI	CTGGAG	2 cut(s)	540, 1097
HaeIII	GGCC	5 cut(s)	498, 548, 957, 1055, 1181
HapII	CCGG	2 cut(s)	155, 1028
HhaI	GCGC	1 cut(s)	824
Hin1II	CATG	7 cut(s)	465, 650, 683, 814, 913, 921, 1228
Hin6I	GCGC	1 cut(s)	822
HinP1I	GCGC	1 cut(s)	822
HincII	GTYRAC	1 cut(s)	145
HindII	GTYRAC	1 cut(s)	145
HindIII	AAGCTT	1 cut(s)	1061
HinfI	GANTC	3 cut(s)	34, 854, 913
HpaI	GTTAAC	1 cut(s)	145
HpaII	CCGG	2 cut(s)	155, 1028
HphI	GGTGA	3 cut(s)	232, 467, 1094
Hpy166II	GTNNAC	4 cut(s)	145, 344, 366, 866
Hpy188I	TCNGA	2 cut(s)	230, 417
Hpy188III	TCNNGA	6 cut(s)	70, 175, 557, 598, 739, 1076
Hpy8I	GTNNAC	4 cut(s)	145, 344, 366, 866
HpyAV	CCTTC	2 cut(s)	1082, 1167
HpyCH4III	ACNGT	2 cut(s)	106, 1011
HpyCH4IV	ACGT	2 cut(s)	531, 1049
HpyCH4V	TGCA	6 cut(s)	77, 512, 891, 921, 1007, 1154
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1123
HpyF3I	CTNAG	2 cut(s)	59, 723
HpySE526I	ACGT	2 cut(s)	531, 1049
Hsp92II	CATG	7 cut(s)	465, 650, 683, 814, 913, 921, 1228
HspAI	GCGC	1 cut(s)	822
KpnI	GGTACC	1 cut(s)	567
KroI	GCCGGC	1 cut(s)	154
KroNI	GCCGGC	1 cut(s)	156
KspAI	GTTAAC	1 cut(s)	145
Kzo9I	GATC	2 cut(s)	735, 846
LguI	GCTCTTC	1 cut(s)	663
LmnI	GCTCC	1 cut(s)	1116
Lsp1109I	GCAGC	1 cut(s)	875
LweI	GCATC	2 cut(s)	338, 895
MaeI	CTAG	2 cut(s)	606, 806
MaeII	ACGT	2 cut(s)	531, 1049
MaeIII	GTNAC	3 cut(s)	54, 455, 1045
MalI	GATC	2 cut(s)	737, 848
MbiI	CCGCTC	1 cut(s)	425
MboI	GATC	2 cut(s)	735, 846
MboII	GAAGA	9 cut(s)	113, 671, 674, 680, 695, 702, 770, 865, 868
MfeI	CAATTG	1 cut(s)	369
MflI	RGATCY	2 cut(s)	735, 846
MhlI	GDGCHC	1 cut(s)	605
MlsI	TGGCCA	1 cut(s)	498
MluCI	AATT	4 cut(s)	309, 369, 706, 1156
MluNI	TGGCCA	1 cut(s)	498
MlyI	GAGTC	1 cut(s)	43
MmeI	TCCRAC	1 cut(s)	468
Mox20I	TGGCCA	1 cut(s)	498
Mph1103I	ATGCAT	1 cut(s)	923
MroNI	GCCGGC	1 cut(s)	154
MscI	TGGCCA	1 cut(s)	498
MseI	TTAA	4 cut(s)	144, 836, 1160, 1264
Msp20I	TGGCCA	1 cut(s)	498
MspA1I	CMGCKG	2 cut(s)	888, 1187
MspI	CCGG	2 cut(s)	155, 1028
MspR9I	CCNGG	3 cut(s)	244, 960, 1028
MunI	CAATTG	1 cut(s)	369
Mva1269I	GAATGC	1 cut(s)	921
MvaI	CCWGG	2 cut(s)	244, 960
MwoI	GCNNNNNNNGC	1 cut(s)	1123
NaeI	GCCGGC	1 cut(s)	156
NciI	CCSGG	1 cut(s)	1028
NcoI	CCATGG	1 cut(s)	1224
NdeI	CATATG	1 cut(s)	540
NdeII	GATC	2 cut(s)	735, 846
NgoMIV	GCCGGC	1 cut(s)	154
NlaIII	CATG	7 cut(s)	465, 650, 683, 814, 913, 921, 1228
NlaIV	GGNNCC	3 cut(s)	549, 565, 1023
NmuCI	GTSAC	1 cut(s)	455
NsiI	ATGCAT	1 cut(s)	923
NspI	RCATGY	1 cut(s)	465
NspV	TTCGAA	1 cut(s)	982
PaeR7I	CTCGAG	1 cut(s)	257
PasI	CCCWGGG	1 cut(s)	959
PciI	ACATGT	1 cut(s)	461
PciSI	GCTCTTC	1 cut(s)	663
PctI	GAATGC	1 cut(s)	921
PdiI	GCCGGC	1 cut(s)	156
PfeI	GAWTC	2 cut(s)	854, 913
PkrI	GCNGC	6 cut(s)	424, 890, 941, 994, 1183, 1186
PleI	GAGTC	1 cut(s)	42
PpsI	GAGTC	1 cut(s)	42
Ppu21I	YACGTR	1 cut(s)	532
PscI	ACATGT	1 cut(s)	461
Psp1406I	AACGTT	1 cut(s)	1049
Psp6I	CCWGG	2 cut(s)	242, 958
PspFI	CCCAGC	2 cut(s)	222, 1229
PspGI	CCWGG	2 cut(s)	242, 958
PspN4I	GGNNCC	3 cut(s)	549, 565, 1023
PspPI	GGNCC	4 cut(s)	547, 863, 956, 1021
PspXI	VCTCGAGB	1 cut(s)	257
PsuI	RGATCY	2 cut(s)	735, 846
PvuII	CAGCTG	1 cut(s)	888
RsaI	GTAC	4 cut(s)	64, 375, 469, 565
RsaNI	GTAC	4 cut(s)	63, 374, 468, 564
SapI	GCTCTTC	1 cut(s)	663
SaqAI	TTAA	4 cut(s)	144, 836, 1160, 1264
SatI	GCNGC	6 cut(s)	423, 889, 940, 993, 1182, 1185
Sau3AI	GATC	2 cut(s)	735, 846
Sau96I	GGNCC	4 cut(s)	547, 863, 956, 1021
SchI	GAGTC	1 cut(s)	43
ScrFI	CCNGG	3 cut(s)	244, 960, 1028
SduI	GDGCHC	1 cut(s)	605
SfaNI	GCATC	2 cut(s)	338, 895
Sfr274I	CTCGAG	1 cut(s)	257
SfuI	TTCGAA	1 cut(s)	982
SinI	GGWCC	2 cut(s)	863, 1021
SlaI	CTCGAG	1 cut(s)	257
SmlI	CTYRAG	1 cut(s)	257
SmoI	CTYRAG	1 cut(s)	257
SnaBI	TACGTA	1 cut(s)	532
Sse9I	AATT	4 cut(s)	309, 369, 706, 1156
SsiI	CCGC	8 cut(s)	327, 423, 861, 939, 993, 1182, 1185, 1214
SspMI	CTAG	2 cut(s)	606, 806
StyD4I	CCNGG	3 cut(s)	242, 958, 1026
StyI	CCWWGG	3 cut(s)	817, 898, 1224
TaaI	ACNGT	2 cut(s)	106, 1011
TaiI	ACGT	2 cut(s)	534, 1052
TaqI	TCGA	2 cut(s)	258, 982
TasI	AATT	4 cut(s)	309, 369, 706, 1156
TatI	WGTACW	3 cut(s)	62, 373, 467
TauI	GCSGC	5 cut(s)	425, 942, 995, 1184, 1187
TfiI	GAWTC	2 cut(s)	854, 913
Tru1I	TTAA	4 cut(s)	144, 836, 1160, 1264
Tru9I	TTAA	4 cut(s)	144, 836, 1160, 1264
TscAI	CASTG	2 cut(s)	506, 886
TseFI	GTSAC	1 cut(s)	455
TseI	GCWGC	1 cut(s)	888
Tsp45I	GTSAC	1 cut(s)	455
TspDTI	ATGAA	4 cut(s)	527, 696, 770, 1193
TspGWI	ACGGA	1 cut(s)	195
TspRI	CASTG	2 cut(s)	506, 886
VpaK11BI	GGWCC	2 cut(s)	863, 1021
XapI	RAATTY	2 cut(s)	706, 1156
XceI	RCATGY	1 cut(s)	465
XhoI	CTCGAG	1 cut(s)	257
XspI	CTAG	2 cut(s)	606, 806
Zsp2I	ATGCAT	1 cut(s)	923

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.