RchiOBHm_Chr4g0399931

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003304
Physical Location & Seq
Forward (+)
17248283 .. 17252571
4289 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37203

Sequence Viewer

Length: 708 bp
ATGCTCGAGTGGTGGTTTATTCCTACGAATGCAGTTCTTGGTAGCATATCAGACTCAATCATAGGTTATATAACCGCATCCATTGTCCGTCCACCATACCCATACTTCAAGTTCACAATTGTACAAATTGGAATTGGAAACATTGGGAATGTGCCGCTTGTTCTGATTGCAGCTTTATGTAGAGACTCATCCAACCCTTTTGGTGACTCGACAACATGTAGCACAAATGGGACTGCCTATATTTTGTTTGGCCAGGTTGGTGCAATCATCCTTTACACATATGTATTTCATATGTTGGCCCCCCCTCCGGATGGTACCTTTGACATTGATGAGGAAACTCTCCCAATCAAGAGCCCTAGTGATGGAGAGACACTTGGACAAGTTCCTTTGCTTACACCTGAAGACAAAGAAGAAAAAGAAGAGCAAAAAAATGAAGAGCAAGTTGTAGAAGTCAACTCAAATTCTTCAAAGAAATCTAAGGTTGATTTACTGTCCAAGGGGACTAATTTTAATTGTATTGGTGATTGGGTTGTGGAGGCCACTGTAAATAGTTGGAGTTGCACAAAAGGAAAAGAAGAGGCAGGATGTGTATGTTATGGTGAGCTTCTTGATGGGAATCCTGGAAGTTTACTTAGTACTGAGCAGCAAATCCAGGCTCCAGCTAATTTAAAAAAGGGCACACACCAAATGCTTTCACAAGCAATATAG

Protein Analysis

235

Amino Acids

25.48

Weight (kDa)

4.5

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 1 - 143 1.1e-21 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 314
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 2 cut(s) 75, 155
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 1 cut(s) 460
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 3 cut(s) 123, 316, 637
AfiI CCNNNNNNNGG 3 cut(s) 307, 311, 362
AflIII ACRYGT 1 cut(s) 215
AgsI TTSAA 2 cut(s) 109, 468
AjnI CCWGG 3 cut(s) 252, 619, 651
AluBI AGCT 3 cut(s) 173, 604, 662
AluI AGCT 3 cut(s) 173, 604, 662
Alw26I GTCTC 2 cut(s) 177, 362
Ama87I CYCGRG 1 cut(s) 5
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 3 cut(s) 250, 297, 537
ApeKI GCWGC 2 cut(s) 170, 643
ApoI RAATTY 1 cut(s) 460
Asp718I GGTACC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 3 cut(s) 215, 533, 611
AvaI CYCGRG 1 cut(s) 5
BaeGI GKGCMC 1 cut(s) 680
BalI TGGCCA 1 cut(s) 252
BanI GGYRCC 1 cut(s) 314
BanII GRGCYC 1 cut(s) 356
BbsI GAAGAC 1 cut(s) 408
BbvI GCAGC 2 cut(s) 182, 655
BccI CCATC 3 cut(s) 305, 356, 605
BciT130I CCWGG 3 cut(s) 254, 621, 653
BcoDI GTCTC 2 cut(s) 177, 362
BfaI CTAG 1 cut(s) 357
BisI GCNGC 3 cut(s) 155, 171, 644
BlsI GCNGC 3 cut(s) 156, 172, 645
BmcAI AGTACT 1 cut(s) 637
Bme1390I CCNGG 3 cut(s) 254, 621, 653
BmeT110I CYCGRG 1 cut(s) 5
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 3 cut(s) 300, 316, 657
BmrFI CCNGG 3 cut(s) 254, 621, 653
BmsI GCATC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 642
BsaBI GATNNNNATC 1 cut(s) 615
BsaJI CCNNGG 1 cut(s) 495
BsaWI WCCGGW 1 cut(s) 307
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 311, 362
Bse8I GATNNNNATC 1 cut(s) 615
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 3 cut(s) 254, 621, 653
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 5 cut(s) 77, 188, 267, 316, 590
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 3 cut(s) 307, 311, 362
BseMII CTCAG 1 cut(s) 630
BseSI GKGCMC 1 cut(s) 680
BseXI GCAGC 2 cut(s) 182, 655
BshFI GGCC 3 cut(s) 252, 299, 539
BshNI GGYRCC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 5
BsiSI CCGG 1 cut(s) 308
BslFI GGGAC 2 cut(s) 244, 514
BslI CCNNNNNNNGG 3 cut(s) 307, 311, 362
BsmAI GTCTC 2 cut(s) 177, 362
BsmFI GGGAC 2 cut(s) 244, 514
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 3 cut(s) 252, 299, 539
BsoBI CYCGRG 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 356, 680
Bsp13I TCCGGA 1 cut(s) 307
Bsp1407I TGTACA 1 cut(s) 121
BspACI CCGC 2 cut(s) 75, 155
BspANI GGCC 3 cut(s) 252, 299, 539
BspCNI CTCAG 1 cut(s) 631
BspEI TCCGGA 1 cut(s) 307
BspLI GGNNCC 3 cut(s) 300, 316, 657
BspQI GCTCTTC 2 cut(s) 414, 429
BspT107I GGYRCC 1 cut(s) 314
BsrGI TGTACA 1 cut(s) 121
BssECI CCNNGG 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 3 cut(s) 254, 621, 653
Bst4CI ACNGT 2 cut(s) 492, 544
Bst6I CTCTTC 3 cut(s) 414, 429, 570
BstAUI TGTACA 1 cut(s) 121
BstDEI CTNAG 3 cut(s) 477, 632, 639
BstF5I GGATG 5 cut(s) 77, 188, 267, 316, 590
BstMAI GTCTC 2 cut(s) 177, 362
BstNI CCWGG 3 cut(s) 254, 621, 653
BstNSI RCATGY 1 cut(s) 219
BstSCI CCNGG 3 cut(s) 252, 619, 651
BstSLI GKGCMC 1 cut(s) 680
BstV1I GCAGC 2 cut(s) 182, 655
BstV2I GAAGAC 1 cut(s) 408
BsuRI GGCC 3 cut(s) 252, 299, 539
BtsCI GGATG 5 cut(s) 77, 188, 267, 316, 590
BtsIMutI CAGTG 1 cut(s) 540
Cfr13I GGNCC 1 cut(s) 298
Csp6I GTAC 3 cut(s) 122, 315, 636
CviAII CATG 1 cut(s) 216
CviJI RGCY 8 cut(s) 173, 252, 299, 354, 539, 604, 656, 662
CviKI_1 RGCY 8 cut(s) 173, 252, 299, 354, 539, 604, 656, 662
CviQI GTAC 3 cut(s) 122, 315, 636
DdeI CTNAG 3 cut(s) 477, 632, 639
DraI TTTAAA 1 cut(s) 669
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 3 cut(s) 414, 429, 570
EarI CTCTTC 3 cut(s) 414, 429, 570
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 5
EcoRII CCWGG 3 cut(s) 252, 619, 651
EcoT14I CCWWGG 1 cut(s) 495
EcoT38I GRGCYC 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 1 cut(s) 219
FaqI GGGAC 2 cut(s) 244, 514
FatI CATG 1 cut(s) 215
FauNDI CATATG 2 cut(s) 280, 291
Fnu4HI GCNGC 3 cut(s) 155, 171, 644
FokI GGATG 5 cut(s) 64, 175, 254, 323, 597
FriOI GRGCYC 1 cut(s) 356
Fsp4HI GCNGC 3 cut(s) 155, 171, 644
FspBI CTAG 1 cut(s) 357
GluI GCNGC 3 cut(s) 155, 171, 644
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 3 cut(s) 252, 299, 539
HapII CCGG 1 cut(s) 308
Hin1II CATG 1 cut(s) 219
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 4 cut(s) 53, 185, 206, 616
HpaII CCGG 1 cut(s) 308
HphI GGTGA 3 cut(s) 215, 533, 611
Hpy166II GTNNAC 4 cut(s) 92, 114, 454, 629
Hpy188I TCNGA 2 cut(s) 52, 165
Hpy188III TCNNGA 3 cut(s) 308, 349, 608
Hpy8I GTNNAC 4 cut(s) 92, 114, 454, 629
HpyCH4III ACNGT 2 cut(s) 492, 544
HpyCH4V TGCA 4 cut(s) 32, 170, 263, 561
HpyF3I CTNAG 3 cut(s) 477, 632, 639
Hsp92II CATG 1 cut(s) 219
Kpn2I TCCGGA 1 cut(s) 307
KpnI GGTACC 1 cut(s) 318
LguI GCTCTTC 2 cut(s) 414, 429
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 2 cut(s) 182, 655
LweI GCATC 1 cut(s) 86
MaeI CTAG 1 cut(s) 357
MaeIII GTNAC 1 cut(s) 203
MboII GAAGA 6 cut(s) 413, 422, 431, 446, 456, 587
MfeI CAATTG 1 cut(s) 117
MhlI GDGCHC 2 cut(s) 356, 680
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 7 cut(s) 117, 126, 132, 460, 505, 511, 664
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 3 cut(s) 47, 179, 200
MmeI TCCRAC 2 cut(s) 216, 533
MnlI CCTC 4 cut(s) 315, 325, 529, 571
Mox20I TGGCCA 1 cut(s) 252
MroI TCCGGA 1 cut(s) 307
MscI TGGCCA 1 cut(s) 252
MseI TTAA 2 cut(s) 510, 668
Msp20I TGGCCA 1 cut(s) 252
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 3 cut(s) 254, 621, 653
MunI CAATTG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 34
MvaI CCWGG 3 cut(s) 254, 621, 653
NdeI CATATG 2 cut(s) 280, 291
NlaIII CATG 1 cut(s) 219
NlaIV GGNNCC 3 cut(s) 300, 316, 657
NmuCI GTSAC 1 cut(s) 203
NspI RCATGY 1 cut(s) 219
PaeR7I CTCGAG 1 cut(s) 5
PciI ACATGT 1 cut(s) 215
PciSI GCTCTTC 2 cut(s) 414, 429
PctI GAATGC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 616
PfoI TCCNGGA 1 cut(s) 619
PkrI GCNGC 3 cut(s) 156, 172, 645
PleI GAGTC 3 cut(s) 47, 179, 200
PpsI GAGTC 3 cut(s) 47, 179, 200
PscI ACATGT 1 cut(s) 215
Psp6I CCWGG 3 cut(s) 252, 619, 651
PspGI CCWGG 3 cut(s) 252, 619, 651
PspN4I GGNNCC 3 cut(s) 300, 316, 657
PspPI GGNCC 1 cut(s) 298
PspXI VCTCGAGB 1 cut(s) 5
RsaI GTAC 3 cut(s) 123, 316, 637
RsaNI GTAC 3 cut(s) 122, 315, 636
SapI GCTCTTC 2 cut(s) 414, 429
SaqAI TTAA 2 cut(s) 510, 668
SatI GCNGC 3 cut(s) 155, 171, 644
Sau96I GGNCC 1 cut(s) 298
ScaI AGTACT 1 cut(s) 637
SchI GAGTC 3 cut(s) 47, 179, 200
ScrFI CCNGG 3 cut(s) 254, 621, 653
SduI GDGCHC 2 cut(s) 356, 680
SetI ASST 8 cut(s) 67, 175, 258, 320, 400, 483, 606, 664
SfaNI GCATC 1 cut(s) 86
Sfr274I CTCGAG 1 cut(s) 5
SlaI CTCGAG 1 cut(s) 5
SmlI CTYRAG 1 cut(s) 5
SmoI CTYRAG 1 cut(s) 5
Sse9I AATT 7 cut(s) 117, 126, 132, 460, 505, 511, 664
SsiI CCGC 2 cut(s) 75, 155
SspMI CTAG 1 cut(s) 357
StyD4I CCNGG 3 cut(s) 252, 619, 651
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 2 cut(s) 492, 544
TaqI TCGA 2 cut(s) 6, 209
TasI AATT 7 cut(s) 117, 126, 132, 460, 505, 511, 664
TatI WGTACW 2 cut(s) 121, 635
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 1 cut(s) 616
Tru1I TTAA 2 cut(s) 510, 668
Tru9I TTAA 2 cut(s) 510, 668
TscAI CASTG 1 cut(s) 547
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 2 cut(s) 170, 643
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 2 cut(s) 278, 447
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 1 cut(s) 547
XapI RAATTY 1 cut(s) 460
XceI RCATGY 1 cut(s) 219
XhoI CTCGAG 1 cut(s) 5
XspI CTAG 1 cut(s) 357
ZrmI AGTACT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.