Rh3BG099100

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0003304
Physical Location & Seq
Forward (+)
7929350 .. 7934067
4718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG099100.1

Sequence Viewer

Length: 1464 bp
ATGGAGAGGATTTTGACAGTGGTGGATATGGGGAATCGAGTCGGAGGGGAGTCGTTACTCAGTACCATCAAGATTGCAGTACTGCCCATAGCAAAGGTTTTTACAGTATGTTCTTTGGGTCTTCTTATGGCTTCCAAGTATGTTAACATCTTGCCGGCAAATGGAAGGAAGCTCTTGAATGGGCTCGTTTTCTCGCTTTTGCTTCCATGTTTGATATTCTCTCAACTCGGACAAGCGATCACTTGGGAGAAAATGCTCGAGTGGTGGTTTATTCCTGCGAATGTAGTTCTTGGTAGCATATCAGGCTCAATCATAGGTTATATAACCGCATCCATCGTCCGTCCACCATACCCATACTTCAAGTTCACAATTGTACAAATTGGAATTGGAAACATTGGGAATGTGCCACTTGTTCTGATTGCAGCTTTATGTAGAGACTCATCCAACCCTTTTGGTGACTCGACCACATGTAGCACAAATGGGACTGCCTATATTTCCTTTGGCCAGTGGGTTGGTGCAATCATCCTTTACACATATGTATTTAATATGTTGGCCCCCCCTCCGGAGGGCACCTTTGACATTGATGAGGAAACTCTCCCAATCAAGAGCCCTACAGATGGAGAGACACTTGGACAAGTTCCTTTGCTTACACATGAAGACCAACAAGAAGAAGAGGAAGAGCAAAAAAATGAAGAGCAAGTTGTAGAAATCAACCCAAATTCTTCAAAGAAATCCATGATTACAGAGTTGTTGATGTTTTTGTATGAGAAATTGAAGCTCAAACAACTTCTTCAACCCCCTATAATAGCTTCTGTCCTAGCCATGGTACTTGGAGCAATACCATTTTTAAAGAAACAGATCTTCGAATCTGGTGGTCCATTTTTCTTCTTCACTGACAGCTGCACTATCCTTGGGGAAGCCATGATTCCATGCATTCTTTTGGCATTAGGCGGCAACCTTATCAATGGCCCAGGAAGTTCCAAACTTGGTCTACGAACAACTGCTGCTATTATATTTTCACGGTTGGTCTTGGTCCCCCCGGTAGGACTTGGGATTGTAACATTGGCTGATAAGCTCGGTTTCCTCCCTGATGGTGATAAGTTGTTTCGTTTTGTCCTACTGCTCCAGCACACAATGCCAACATCTGTTCTTGCTGGTACTCAATCTTACCTTACAGAGCTTAAGTCACATGACTCCTTGCGTTTTTATGTTTGTTCTGCACAATGTTACGCAATGCACATGGCAGTGATTAACAAATCAACCTTATGTTGTAGGTGCTGTTGCAAATTTAAGAGGCTGTGGAAGAGAGGCTGCTGCTGTCTTGTTCTGGGTTCATATATTTGCGGTGTTCTCCATGGCTGGGTGGATTGTTCTGTATCTCAACATACTCTTCTAAAAACATCCGAAATTGGAAGCACCAGTGAGCGCCACAGTTACTCATCTCTCTCGGCCTTCGGCTATTAA

Protein Analysis

487

Amino Acids

53.26

Weight (kDa)

6.99

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 27 - 395 1.1e-82 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 569
AccI GTMKAC 1 cut(s) 991
AccIII TCCGGA 1 cut(s) 562
AciI CCGC 3 cut(s) 327, 951, 1344
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 2 cut(s) 718, 1286
AfaI GTAC 5 cut(s) 64, 81, 375, 828, 1159
AfiI CCNNNNNNNGG 8 cut(s) 161, 562, 565, 566, 617, 823, 1043, 1360
AflII CTTAAG 1 cut(s) 1181
AflIII ACRYGT 1 cut(s) 467
AgsI TTSAA 5 cut(s) 178, 361, 726, 775, 794
AjnI CCWGG 1 cut(s) 970
AluBI AGCT 7 cut(s) 172, 425, 778, 809, 900, 1075, 1180
AluI AGCT 7 cut(s) 172, 425, 778, 809, 900, 1075, 1180
Alw26I GTCTC 2 cut(s) 429, 617
Ama87I CYCGRG 1 cut(s) 257
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 4 cut(s) 502, 552, 967, 1449
ApeKI GCWGC 5 cut(s) 422, 900, 1004, 1311, 1314
ApoI RAATTY 2 cut(s) 718, 1286
AspLEI GCGC 1 cut(s) 1428
AspS9I GGNCC 4 cut(s) 553, 875, 968, 1033
AsuC2I CCSGG 1 cut(s) 1040
AsuHPI GGTGA 2 cut(s) 467, 1106
AsuII TTCGAA 1 cut(s) 864
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 2 cut(s) 875, 1033
BaeGI GKGCMC 1 cut(s) 572
BalI TGGCCA 1 cut(s) 504
BanI GGYRCC 1 cut(s) 569
BanII GRGCYC 2 cut(s) 186, 611
BbsI GAAGAC 2 cut(s) 113, 663
BbvI GCAGC 5 cut(s) 434, 887, 991, 1298, 1301
BccI CCATC 4 cut(s) 74, 341, 611, 1085
BciT130I CCWGG 1 cut(s) 972
BcnI CCSGG 1 cut(s) 1040
BcoDI GTCTC 2 cut(s) 429, 617
BfaI CTAG 1 cut(s) 818
BfmI CTRYAG 1 cut(s) 612
BfoI RGCGCY 1 cut(s) 1429
BfrI CTTAAG 1 cut(s) 1181
BglII AGATCT 1 cut(s) 858
BisI GCNGC 6 cut(s) 423, 901, 952, 1005, 1312, 1315
BlsI GCNGC 6 cut(s) 424, 902, 953, 1006, 1313, 1316
BmcAI AGTACT 1 cut(s) 81
Bme1390I CCNGG 2 cut(s) 972, 1040
Bme18I GGWCC 2 cut(s) 875, 1033
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 4 cut(s) 553, 875, 968, 1033
BmiI GGNNCC 3 cut(s) 555, 571, 1035
BmrFI CCNGG 2 cut(s) 972, 1040
BmsI GCATC 1 cut(s) 338
BpiI GAAGAC 2 cut(s) 113, 663
BpmI CTGGAG 1 cut(s) 1109
Bpu14I TTCGAA 1 cut(s) 864
BpuMI CCSGG 1 cut(s) 1040
BsaJI CCNNGG 5 cut(s) 822, 910, 970, 1038, 1354
BsaWI WCCGGW 1 cut(s) 562
Bsc4I CCNNNNNNNGG 8 cut(s) 161, 562, 565, 566, 617, 823, 1043, 1360
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 2 cut(s) 505, 1419
Bse3DI GCAATG 1 cut(s) 1239
BseAI TCCGGA 1 cut(s) 562
BseBI CCWGG 1 cut(s) 972
BseDI CCNNGG 5 cut(s) 822, 910, 970, 1038, 1354
BseGI GGATG 4 cut(s) 329, 440, 522, 1400
BseLI CCNNNNNNNGG 8 cut(s) 161, 562, 565, 566, 617, 823, 1043, 1360
BseMI GCAATG 1 cut(s) 1239
BseMII CTCAG 1 cut(s) 73
BseNI ACTGG 2 cut(s) 505, 1419
BseSI GKGCMC 1 cut(s) 572
BseXI GCAGC 5 cut(s) 434, 887, 991, 1298, 1301
BseYI CCCAGC 1 cut(s) 1359
BsgI GTGCAG 2 cut(s) 886, 1203
BshFI GGCC 4 cut(s) 504, 554, 969, 1451
BshNI GGYRCC 1 cut(s) 569
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 3 cut(s) 155, 563, 1040
BslFI GGGAC 2 cut(s) 496, 1019
BslI CCNNNNNNNGG 8 cut(s) 161, 562, 565, 566, 617, 823, 1043, 1360
BsmAI GTCTC 2 cut(s) 429, 617
BsmFI GGGAC 2 cut(s) 496, 1019
BsmI GAATGC 1 cut(s) 933
BsnI GGCC 4 cut(s) 504, 554, 969, 1451
BsoBI CYCGRG 1 cut(s) 257
Bsp119I TTCGAA 1 cut(s) 864
Bsp1286I GDGCHC 3 cut(s) 186, 572, 611
Bsp13I TCCGGA 1 cut(s) 562
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 2 cut(s) 237, 858
Bsp19I CCATGG 2 cut(s) 822, 1354
BspACI CCGC 3 cut(s) 327, 951, 1344
BspANI GGCC 4 cut(s) 504, 554, 969, 1451
BspCNI CTCAG 1 cut(s) 72
BspEI TCCGGA 1 cut(s) 562
BspLI GGNNCC 3 cut(s) 555, 571, 1035
BspQI GCTCTTC 2 cut(s) 672, 687
BspT104I TTCGAA 1 cut(s) 864
BspT107I GGYRCC 1 cut(s) 569
BspTI CTTAAG 1 cut(s) 1181
BsrDI GCAATG 1 cut(s) 1239
BsrFI RCCGGY 1 cut(s) 154
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 2 cut(s) 505, 1419
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 5 cut(s) 822, 910, 970, 1038, 1354
BssMI GATC 2 cut(s) 237, 858
BssT1I CCWWGG 3 cut(s) 822, 910, 1354
Bst2UI CCWGG 1 cut(s) 972
Bst4CI ACNGT 4 cut(s) 19, 106, 1023, 1433
Bst6I CTCTTC 5 cut(s) 666, 672, 687, 1298, 1395
BstAFI CTTAAG 1 cut(s) 1181
BstAPI GCANNNNNTGC 1 cut(s) 1135
BstAUI TGTACA 1 cut(s) 373
BstBI TTCGAA 1 cut(s) 864
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 2 cut(s) 822, 1354
BstF5I GGATG 4 cut(s) 329, 440, 522, 1400
BstH2I RGCGCY 1 cut(s) 1429
BstHHI GCGC 1 cut(s) 1428
BstKTI GATC 2 cut(s) 240, 861
BstMAI GTCTC 2 cut(s) 429, 617
BstMBI GATC 2 cut(s) 237, 858
BstMWI GCNNNNNNNGC 2 cut(s) 303, 1135
BstNI CCWGG 1 cut(s) 972
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 970, 1038
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 1 cut(s) 572
BstV1I GCAGC 5 cut(s) 434, 887, 991, 1298, 1301
BstV2I GAAGAC 2 cut(s) 113, 663
BstX2I RGATCY 1 cut(s) 858
BstXI CCANNNNNNTGG 1 cut(s) 512
BstYI RGATCY 1 cut(s) 858
BsuRI GGCC 4 cut(s) 504, 554, 969, 1451
BtgI CCRYGG 2 cut(s) 822, 1354
BtsCI GGATG 4 cut(s) 329, 440, 522, 1400
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 5 cut(s) 24, 512, 891, 1251, 1426
Cac8I GCNNGC 1 cut(s) 156
CfoI GCGC 1 cut(s) 1428
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 4 cut(s) 553, 875, 968, 1033
Csp6I GTAC 5 cut(s) 63, 80, 374, 827, 1158
CviQI GTAC 5 cut(s) 63, 80, 374, 827, 1158
DdeI CTNAG 1 cut(s) 59
DpnI GATC 2 cut(s) 239, 860
DpnII GATC 2 cut(s) 237, 858
DraI TTTAAA 1 cut(s) 849
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 5 cut(s) 666, 672, 687, 1298, 1395
EarI CTCTTC 5 cut(s) 666, 672, 687, 1298, 1395
Eco130I CCWWGG 3 cut(s) 822, 910, 1354
Eco24I GRGCYC 2 cut(s) 186, 611
Eco47I GGWCC 2 cut(s) 875, 1033
Eco88I CYCGRG 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 970
EcoT14I CCWWGG 3 cut(s) 822, 910, 1354
EcoT22I ATGCAT 1 cut(s) 935
EcoT38I GRGCYC 2 cut(s) 186, 611
ErhI CCWWGG 3 cut(s) 822, 910, 1354
FaqI GGGAC 2 cut(s) 496, 1019
FauNDI CATATG 1 cut(s) 535
FblI GTMKAC 1 cut(s) 991
Fnu4HI GCNGC 6 cut(s) 423, 901, 952, 1005, 1312, 1315
FokI GGATG 4 cut(s) 316, 427, 509, 1387
FriOI GRGCYC 2 cut(s) 186, 611
Fsp4HI GCNGC 6 cut(s) 423, 901, 952, 1005, 1312, 1315
FspBI CTAG 1 cut(s) 818
GlaI GCGC 1 cut(s) 1427
GluI GCNGC 6 cut(s) 423, 901, 952, 1005, 1312, 1315
GsaI CCCAGC 1 cut(s) 1363
GsuI CTGGAG 1 cut(s) 1109
HaeII RGCGCY 1 cut(s) 1429
HaeIII GGCC 4 cut(s) 504, 554, 969, 1451
HapII CCGG 3 cut(s) 155, 563, 1040
HhaI GCGC 1 cut(s) 1428
Hin6I GCGC 1 cut(s) 1426
HinP1I GCGC 1 cut(s) 1426
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 8 cut(s) 34, 39, 50, 437, 458, 866, 925, 1193
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 3 cut(s) 155, 563, 1040
HphI GGTGA 2 cut(s) 467, 1106
Hpy166II GTNNAC 4 cut(s) 145, 344, 366, 992
Hpy188I TCNGA 4 cut(s) 44, 230, 417, 1405
Hpy188III TCNNGA 4 cut(s) 70, 175, 563, 604
Hpy8I GTNNAC 4 cut(s) 145, 344, 366, 992
HpyAV CCTTC 2 cut(s) 159, 1462
HpyCH4III ACNGT 4 cut(s) 19, 106, 1023, 1433
HpyCH4V TGCA 8 cut(s) 77, 422, 518, 903, 933, 1220, 1237, 1284
HpyF10VI GCNNNNNNNGC 2 cut(s) 303, 1135
HpyF3I CTNAG 1 cut(s) 59
HspAI GCGC 1 cut(s) 1426
Kpn2I TCCGGA 1 cut(s) 562
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 2 cut(s) 237, 858
LguI GCTCTTC 2 cut(s) 672, 687
LmnI GCTCC 2 cut(s) 833, 1128
Lsp1109I GCAGC 5 cut(s) 434, 887, 991, 1298, 1301
LweI GCATC 1 cut(s) 338
MaeI CTAG 1 cut(s) 818
MaeIII GTNAC 6 cut(s) 54, 455, 1057, 1185, 1226, 1433
MalI GATC 2 cut(s) 239, 860
MboI GATC 2 cut(s) 237, 858
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 1 cut(s) 858
MhlI GDGCHC 3 cut(s) 186, 572, 611
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 7 cut(s) 369, 378, 384, 718, 770, 1286, 1407
MluNI TGGCCA 1 cut(s) 504
MlyI GAGTC 5 cut(s) 48, 59, 431, 452, 1187
MmeI TCCRAC 2 cut(s) 22, 468
MnlI CCTC 8 cut(s) 38, 559, 570, 580, 667, 1094, 1287, 1301
Mox20I TGGCCA 1 cut(s) 504
Mph1103I ATGCAT 1 cut(s) 935
MroI TCCGGA 1 cut(s) 562
MroNI GCCGGC 1 cut(s) 154
MscI TGGCCA 1 cut(s) 504
MseI TTAA 7 cut(s) 144, 543, 848, 1182, 1251, 1290, 1462
MslI CAYNNNNRTG 1 cut(s) 1244
Msp20I TGGCCA 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 900
MspCI CTTAAG 1 cut(s) 1181
MspI CCGG 3 cut(s) 155, 563, 1040
MspR9I CCNGG 2 cut(s) 972, 1040
MunI CAATTG 1 cut(s) 369
Mva1269I GAATGC 1 cut(s) 933
MvaI CCWGG 1 cut(s) 972
MwoI GCNNNNNNNGC 2 cut(s) 303, 1135
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 1040
NcoI CCATGG 2 cut(s) 822, 1354
NdeI CATATG 1 cut(s) 535
NdeII GATC 2 cut(s) 237, 858
NgoMIV GCCGGC 1 cut(s) 154
NlaIV GGNNCC 3 cut(s) 555, 571, 1035
NmeAIII GCCGAG 1 cut(s) 1427
NmuCI GTSAC 2 cut(s) 455, 1185
NsiI ATGCAT 1 cut(s) 935
NspI RCATGY 1 cut(s) 471
NspV TTCGAA 1 cut(s) 864
PaeR7I CTCGAG 1 cut(s) 257
PciI ACATGT 1 cut(s) 467
PciSI GCTCTTC 2 cut(s) 672, 687
PctI GAATGC 1 cut(s) 933
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 3 cut(s) 34, 866, 925
PkrI GCNGC 6 cut(s) 424, 902, 953, 1006, 1313, 1316
PleI GAGTC 5 cut(s) 47, 58, 431, 452, 1187
PpsI GAGTC 5 cut(s) 47, 58, 431, 452, 1187
PscI ACATGT 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 970
PspFI CCCAGC 1 cut(s) 1359
PspGI CCWGG 1 cut(s) 970
PspN4I GGNNCC 3 cut(s) 555, 571, 1035
PspPI GGNCC 4 cut(s) 553, 875, 968, 1033
PspXI VCTCGAGB 1 cut(s) 257
PsuI RGATCY 1 cut(s) 858
PvuII CAGCTG 1 cut(s) 900
RsaI GTAC 5 cut(s) 64, 81, 375, 828, 1159
RsaNI GTAC 5 cut(s) 63, 80, 374, 827, 1158
RseI CAYNNNNRTG 1 cut(s) 1244
SapI GCTCTTC 2 cut(s) 672, 687
SaqAI TTAA 7 cut(s) 144, 543, 848, 1182, 1251, 1290, 1462
SatI GCNGC 6 cut(s) 423, 901, 952, 1005, 1312, 1315
Sau3AI GATC 2 cut(s) 237, 858
Sau96I GGNCC 4 cut(s) 553, 875, 968, 1033
ScaI AGTACT 1 cut(s) 81
SchI GAGTC 5 cut(s) 48, 59, 431, 452, 1187
ScrFI CCNGG 2 cut(s) 972, 1040
SduI GDGCHC 3 cut(s) 186, 572, 611
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 1 cut(s) 612
Sfr274I CTCGAG 1 cut(s) 257
SfuI TTCGAA 1 cut(s) 864
SinI GGWCC 2 cut(s) 875, 1033
SlaI CTCGAG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 1244
SmlI CTYRAG 2 cut(s) 257, 1181
SmoI CTYRAG 2 cut(s) 257, 1181
Sse9I AATT 7 cut(s) 369, 378, 384, 718, 770, 1286, 1407
SsiI CCGC 3 cut(s) 327, 951, 1344
SspMI CTAG 1 cut(s) 818
StyD4I CCNGG 2 cut(s) 970, 1038
StyI CCWWGG 3 cut(s) 822, 910, 1354
TaaI ACNGT 4 cut(s) 19, 106, 1023, 1433
TaqI TCGA 4 cut(s) 37, 258, 461, 864
TasI AATT 7 cut(s) 369, 378, 384, 718, 770, 1286, 1407
TatI WGTACW 2 cut(s) 79, 373
TauI GCSGC 1 cut(s) 954
TfiI GAWTC 3 cut(s) 34, 866, 925
Tru1I TTAA 7 cut(s) 144, 543, 848, 1182, 1251, 1290, 1462
Tru9I TTAA 7 cut(s) 144, 543, 848, 1182, 1251, 1290, 1462
TscAI CASTG 5 cut(s) 24, 512, 898, 1251, 1426
TseFI GTSAC 2 cut(s) 455, 1185
TseI GCWGC 5 cut(s) 422, 900, 1004, 1311, 1314
Tsp45I GTSAC 2 cut(s) 455, 1185
TspDTI ATGAA 3 cut(s) 669, 705, 1323
TspGWI ACGGA 1 cut(s) 329
TspRI CASTG 5 cut(s) 24, 512, 898, 1251, 1426
Vha464I CTTAAG 1 cut(s) 1181
VpaK11BI GGWCC 2 cut(s) 875, 1033
XapI RAATTY 2 cut(s) 718, 1286
XceI RCATGY 1 cut(s) 471
XhoI CTCGAG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 991
XspI CTAG 1 cut(s) 818
ZrmI AGTACT 1 cut(s) 81
Zsp2I ATGCAT 1 cut(s) 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.