RchiOBHm_Chr3g0459731

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0003304
Physical Location & Seq
Forward (+)
8115755 .. 8120118
4364 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42632

Sequence Viewer

Length: 1233 bp
ATGGAGAGGATTTTGACAGTGGTGGATATGGGGAATCGAGTCGGCGGGGAGTCGTTACTCAGTACCATCAAGATTGCAGTACTGCCCATAGCAAAGGTTTTTACAGTATGTTCTTTGGGTCTTCTTATGGCTTCCAAGTATGTTAACATCTTGCCGGCAAATGGAAGGAAGCTCTTGAATGGGCTCGTTTTCTCGCTTTTGCTTCCATGTTTGATATTCTCTCAACTCGGACAAGCGATCACTTGGGAGAAAATGCTCGAGTGGTGGTTTATTCCTGCGAATGTAGTTCTTGGTAGCATATCAGGCTCAATCATAGGTTATATAACCGCATCCATCGTCCGTCCACCATACCCATACTTCAAGTTCACAATTGTACAAATTGGAATTGGAAACATTGGGAATGTGCCACTTGTTCTGATTGCAGCTTTATGTAGAGACTCATCCAACCCTTTTGGTGACTCGACCACATGTAGCACAAATGGGACTGCCTATATTTCCTTTGGCCAGTGGGTTGGTGCAATCATCCTTTACACATATGTATTTAATATGTTGGCCCCCCCTCCGGAGGGCACCTTTGACATTGATGAGGAAACTCTCCCAATCAAGAGCCCTACAGATGGAGAGACACTTGGACAACAAAAAAATGAAGAGCAAGTTGTAGAAATCAACCCAAATTCTTCAAAGAAATCCATGATTACAGAGTTGTTGATGTTTTTGTATGAGAAATTGAAGCTCAAACAACTTCTTCAACCCCCTATAATAGCTTCTGTCCTAGCCATGGTACTTGGAGCAATACCATTTTTAAAGAAACAGATCTTCGAATCTGGTGGTCCATTTTTCTTCTTCACTGACAGCTGCACTATCCTTGGGGAAGCCATGATTCCATGCATTCTTTTGGCATTAGGCGGCAACCTTATCAATGGCCCAGGAAGTTCCAAACTTGGTCTACGAACAACTGCTGCTATTATATTTTCACGGTTGGTCTTGGTCCCCCCGGTAGGACTTGGGATTGTAACATTGGCTGATAAGCTCGGTTTCCTCCCTGATGGTGATAAGTTGTTTCGTTTTGTCCTACTGCTCCAGCACACAATGCCAACATCTGTTCTTGCTGGTGCTGTTGCAAATTTAAGAGGCTGTGGAAGAGAGGCTGCTGCTGTCTTGTTCTGGGTTCATATATTTGCGGTGTTCTCCATGGCTGGGTGGATTGTTCTGTATCTCAACATACTCTTCTAA

Protein Analysis

410

Amino Acids

44.41

Weight (kDa)

8.09

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 27 - 402 1.2e-91 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 569
AccI GTMKAC 1 cut(s) 946
AccIII TCCGGA 1 cut(s) 562
AciI CCGC 4 cut(s) 45, 327, 906, 1181
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 2 cut(s) 673, 1123
AfaI GTAC 4 cut(s) 64, 81, 375, 783
AfiI CCNNNNNNNGG 8 cut(s) 161, 562, 565, 566, 617, 778, 998, 1197
AflIII ACRYGT 1 cut(s) 467
AgsI TTSAA 5 cut(s) 178, 361, 681, 730, 749
AjnI CCWGG 1 cut(s) 925
AluBI AGCT 6 cut(s) 172, 425, 733, 764, 855, 1030
AluI AGCT 6 cut(s) 172, 425, 733, 764, 855, 1030
Alw26I GTCTC 2 cut(s) 429, 617
Ama87I CYCGRG 1 cut(s) 257
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 3 cut(s) 502, 552, 922
ApeKI GCWGC 5 cut(s) 422, 855, 959, 1148, 1151
ApoI RAATTY 2 cut(s) 673, 1123
AspS9I GGNCC 4 cut(s) 553, 830, 923, 988
AsuC2I CCSGG 1 cut(s) 995
AsuHPI GGTGA 2 cut(s) 467, 1061
AsuII TTCGAA 1 cut(s) 819
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 2 cut(s) 830, 988
BaeGI GKGCMC 1 cut(s) 572
BalI TGGCCA 1 cut(s) 504
BanI GGYRCC 1 cut(s) 569
BanII GRGCYC 2 cut(s) 186, 611
BbsI GAAGAC 1 cut(s) 113
BbvI GCAGC 5 cut(s) 434, 842, 946, 1135, 1138
BccI CCATC 4 cut(s) 74, 341, 611, 1040
BciT130I CCWGG 1 cut(s) 927
BcnI CCSGG 1 cut(s) 995
BcoDI GTCTC 2 cut(s) 429, 617
BfaI CTAG 1 cut(s) 773
BfmI CTRYAG 1 cut(s) 612
BglII AGATCT 1 cut(s) 813
BisI GCNGC 6 cut(s) 423, 856, 907, 960, 1149, 1152
BlsI GCNGC 6 cut(s) 424, 857, 908, 961, 1150, 1153
BmcAI AGTACT 1 cut(s) 81
Bme1390I CCNGG 2 cut(s) 927, 995
Bme18I GGWCC 2 cut(s) 830, 988
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 4 cut(s) 553, 830, 923, 988
BmiI GGNNCC 3 cut(s) 555, 571, 990
BmrFI CCNGG 2 cut(s) 927, 995
BmsI GCATC 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 113
BpmI CTGGAG 1 cut(s) 1064
Bpu14I TTCGAA 1 cut(s) 819
BpuMI CCSGG 1 cut(s) 995
BsaJI CCNNGG 5 cut(s) 777, 865, 925, 993, 1191
BsaWI WCCGGW 1 cut(s) 562
Bsc4I CCNNNNNNNGG 8 cut(s) 161, 562, 565, 566, 617, 778, 998, 1197
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 505
BseAI TCCGGA 1 cut(s) 562
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 5 cut(s) 777, 865, 925, 993, 1191
BseGI GGATG 3 cut(s) 329, 440, 522
BseLI CCNNNNNNNGG 8 cut(s) 161, 562, 565, 566, 617, 778, 998, 1197
BseMII CTCAG 1 cut(s) 73
BseNI ACTGG 1 cut(s) 505
BseSI GKGCMC 1 cut(s) 572
BseXI GCAGC 5 cut(s) 434, 842, 946, 1135, 1138
BseYI CCCAGC 1 cut(s) 1196
BsgI GTGCAG 1 cut(s) 841
BshFI GGCC 3 cut(s) 504, 554, 924
BshNI GGYRCC 1 cut(s) 569
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 3 cut(s) 155, 563, 995
BslFI GGGAC 2 cut(s) 496, 974
BslI CCNNNNNNNGG 8 cut(s) 161, 562, 565, 566, 617, 778, 998, 1197
BsmAI GTCTC 2 cut(s) 429, 617
BsmFI GGGAC 2 cut(s) 496, 974
BsmI GAATGC 1 cut(s) 888
BsnI GGCC 3 cut(s) 504, 554, 924
BsoBI CYCGRG 1 cut(s) 257
Bsp119I TTCGAA 1 cut(s) 819
Bsp1286I GDGCHC 3 cut(s) 186, 572, 611
Bsp13I TCCGGA 1 cut(s) 562
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 2 cut(s) 237, 813
Bsp19I CCATGG 2 cut(s) 777, 1191
BspACI CCGC 4 cut(s) 45, 327, 906, 1181
BspANI GGCC 3 cut(s) 504, 554, 924
BspCNI CTCAG 1 cut(s) 72
BspEI TCCGGA 1 cut(s) 562
BspLI GGNNCC 3 cut(s) 555, 571, 990
BspQI GCTCTTC 1 cut(s) 642
BspT104I TTCGAA 1 cut(s) 819
BspT107I GGYRCC 1 cut(s) 569
BsrFI RCCGGY 1 cut(s) 154
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 1 cut(s) 505
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 5 cut(s) 777, 865, 925, 993, 1191
BssMI GATC 2 cut(s) 237, 813
BssT1I CCWWGG 3 cut(s) 777, 865, 1191
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 3 cut(s) 19, 106, 978
Bst6I CTCTTC 2 cut(s) 642, 1135
BstAPI GCANNNNNTGC 1 cut(s) 1090
BstAUI TGTACA 1 cut(s) 373
BstBI TTCGAA 1 cut(s) 819
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 2 cut(s) 777, 1191
BstF5I GGATG 3 cut(s) 329, 440, 522
BstKTI GATC 2 cut(s) 240, 816
BstMAI GTCTC 2 cut(s) 429, 617
BstMBI GATC 2 cut(s) 237, 813
BstMWI GCNNNNNNNGC 2 cut(s) 303, 1090
BstNI CCWGG 1 cut(s) 927
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 925, 993
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 1 cut(s) 572
BstV1I GCAGC 5 cut(s) 434, 842, 946, 1135, 1138
BstV2I GAAGAC 1 cut(s) 113
BstX2I RGATCY 1 cut(s) 813
BstXI CCANNNNNNTGG 1 cut(s) 512
BstYI RGATCY 1 cut(s) 813
BsuRI GGCC 3 cut(s) 504, 554, 924
BtgI CCRYGG 2 cut(s) 777, 1191
BtsCI GGATG 3 cut(s) 329, 440, 522
BtsIMutI CAGTG 3 cut(s) 24, 512, 846
Cac8I GCNNGC 1 cut(s) 156
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 4 cut(s) 553, 830, 923, 988
Csp6I GTAC 4 cut(s) 63, 80, 374, 782
CviAII CATG 7 cut(s) 207, 468, 691, 778, 877, 885, 1192
CviQI GTAC 4 cut(s) 63, 80, 374, 782
DdeI CTNAG 1 cut(s) 59
DpnI GATC 2 cut(s) 239, 815
DpnII GATC 2 cut(s) 237, 813
DraI TTTAAA 1 cut(s) 804
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 2 cut(s) 642, 1135
EarI CTCTTC 2 cut(s) 642, 1135
Eco130I CCWWGG 3 cut(s) 777, 865, 1191
Eco24I GRGCYC 2 cut(s) 186, 611
Eco47I GGWCC 2 cut(s) 830, 988
Eco88I CYCGRG 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 925
EcoT14I CCWWGG 3 cut(s) 777, 865, 1191
EcoT22I ATGCAT 1 cut(s) 890
EcoT38I GRGCYC 2 cut(s) 186, 611
ErhI CCWWGG 3 cut(s) 777, 865, 1191
FaeI CATG 7 cut(s) 210, 471, 694, 781, 880, 888, 1195
FaqI GGGAC 2 cut(s) 496, 974
FatI CATG 7 cut(s) 206, 467, 690, 777, 876, 884, 1191
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 535
FblI GTMKAC 1 cut(s) 946
Fnu4HI GCNGC 6 cut(s) 423, 856, 907, 960, 1149, 1152
FokI GGATG 3 cut(s) 316, 427, 509
FriOI GRGCYC 2 cut(s) 186, 611
Fsp4HI GCNGC 6 cut(s) 423, 856, 907, 960, 1149, 1152
FspBI CTAG 1 cut(s) 773
GluI GCNGC 6 cut(s) 423, 856, 907, 960, 1149, 1152
GsaI CCCAGC 1 cut(s) 1200
GsuI CTGGAG 1 cut(s) 1064
HaeIII GGCC 3 cut(s) 504, 554, 924
HapII CCGG 3 cut(s) 155, 563, 995
Hin1II CATG 7 cut(s) 210, 471, 694, 781, 880, 888, 1195
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 7 cut(s) 34, 39, 50, 437, 458, 821, 880
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 3 cut(s) 155, 563, 995
HphI GGTGA 2 cut(s) 467, 1061
Hpy166II GTNNAC 4 cut(s) 145, 344, 366, 947
Hpy188I TCNGA 2 cut(s) 230, 417
Hpy188III TCNNGA 4 cut(s) 70, 175, 563, 604
Hpy8I GTNNAC 4 cut(s) 145, 344, 366, 947
HpyAV CCTTC 1 cut(s) 159
HpyCH4III ACNGT 3 cut(s) 19, 106, 978
HpyCH4V TGCA 6 cut(s) 77, 422, 518, 858, 888, 1121
HpyF10VI GCNNNNNNNGC 2 cut(s) 303, 1090
HpyF3I CTNAG 1 cut(s) 59
Hsp92II CATG 7 cut(s) 210, 471, 694, 781, 880, 888, 1195
Kpn2I TCCGGA 1 cut(s) 562
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 2 cut(s) 237, 813
LguI GCTCTTC 1 cut(s) 642
LmnI GCTCC 2 cut(s) 788, 1083
Lsp1109I GCAGC 5 cut(s) 434, 842, 946, 1135, 1138
LweI GCATC 1 cut(s) 338
MaeI CTAG 1 cut(s) 773
MaeIII GTNAC 3 cut(s) 54, 455, 1012
MalI GATC 2 cut(s) 239, 815
MboI GATC 2 cut(s) 237, 813
MboII GAAGA 9 cut(s) 113, 659, 669, 737, 808, 832, 835, 1152, 1219
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 1 cut(s) 813
MhlI GDGCHC 3 cut(s) 186, 572, 611
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 6 cut(s) 369, 378, 384, 673, 725, 1123
MluNI TGGCCA 1 cut(s) 504
MlyI GAGTC 4 cut(s) 48, 59, 431, 452
MmeI TCCRAC 1 cut(s) 468
MnlI CCTC 6 cut(s) 559, 570, 580, 1049, 1124, 1138
Mox20I TGGCCA 1 cut(s) 504
Mph1103I ATGCAT 1 cut(s) 890
MroI TCCGGA 1 cut(s) 562
MroNI GCCGGC 1 cut(s) 154
MscI TGGCCA 1 cut(s) 504
MseI TTAA 4 cut(s) 144, 543, 803, 1127
Msp20I TGGCCA 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 3 cut(s) 155, 563, 995
MspR9I CCNGG 2 cut(s) 927, 995
MunI CAATTG 1 cut(s) 369
Mva1269I GAATGC 1 cut(s) 888
MvaI CCWGG 1 cut(s) 927
MwoI GCNNNNNNNGC 2 cut(s) 303, 1090
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 995
NcoI CCATGG 2 cut(s) 777, 1191
NdeI CATATG 1 cut(s) 535
NdeII GATC 2 cut(s) 237, 813
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 7 cut(s) 210, 471, 694, 781, 880, 888, 1195
NlaIV GGNNCC 3 cut(s) 555, 571, 990
NmuCI GTSAC 1 cut(s) 455
NsiI ATGCAT 1 cut(s) 890
NspI RCATGY 1 cut(s) 471
NspV TTCGAA 1 cut(s) 819
PaeR7I CTCGAG 1 cut(s) 257
PciI ACATGT 1 cut(s) 467
PciSI GCTCTTC 1 cut(s) 642
PctI GAATGC 1 cut(s) 888
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 3 cut(s) 34, 821, 880
PkrI GCNGC 6 cut(s) 424, 857, 908, 961, 1150, 1153
PleI GAGTC 4 cut(s) 47, 58, 431, 452
PpsI GAGTC 4 cut(s) 47, 58, 431, 452
PscI ACATGT 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 925
PspFI CCCAGC 1 cut(s) 1196
PspGI CCWGG 1 cut(s) 925
PspN4I GGNNCC 3 cut(s) 555, 571, 990
PspPI GGNCC 4 cut(s) 553, 830, 923, 988
PspXI VCTCGAGB 1 cut(s) 257
PsuI RGATCY 1 cut(s) 813
PvuII CAGCTG 1 cut(s) 855
RsaI GTAC 4 cut(s) 64, 81, 375, 783
RsaNI GTAC 4 cut(s) 63, 80, 374, 782
SapI GCTCTTC 1 cut(s) 642
SaqAI TTAA 4 cut(s) 144, 543, 803, 1127
SatI GCNGC 6 cut(s) 423, 856, 907, 960, 1149, 1152
Sau3AI GATC 2 cut(s) 237, 813
Sau96I GGNCC 4 cut(s) 553, 830, 923, 988
ScaI AGTACT 1 cut(s) 81
SchI GAGTC 4 cut(s) 48, 59, 431, 452
ScrFI CCNGG 2 cut(s) 927, 995
SduI GDGCHC 3 cut(s) 186, 572, 611
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 1 cut(s) 612
Sfr274I CTCGAG 1 cut(s) 257
SfuI TTCGAA 1 cut(s) 819
SinI GGWCC 2 cut(s) 830, 988
SlaI CTCGAG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 6 cut(s) 369, 378, 384, 673, 725, 1123
SsiI CCGC 4 cut(s) 45, 327, 906, 1181
SspMI CTAG 1 cut(s) 773
StyD4I CCNGG 2 cut(s) 925, 993
StyI CCWWGG 3 cut(s) 777, 865, 1191
TaaI ACNGT 3 cut(s) 19, 106, 978
TaqI TCGA 4 cut(s) 37, 258, 461, 819
TasI AATT 6 cut(s) 369, 378, 384, 673, 725, 1123
TatI WGTACW 2 cut(s) 79, 373
TauI GCSGC 1 cut(s) 909
TfiI GAWTC 3 cut(s) 34, 821, 880
Tru1I TTAA 4 cut(s) 144, 543, 803, 1127
Tru9I TTAA 4 cut(s) 144, 543, 803, 1127
TscAI CASTG 3 cut(s) 24, 512, 853
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 5 cut(s) 422, 855, 959, 1148, 1151
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 2 cut(s) 660, 1160
TspGWI ACGGA 1 cut(s) 329
TspRI CASTG 3 cut(s) 24, 512, 853
VpaK11BI GGWCC 2 cut(s) 830, 988
XapI RAATTY 2 cut(s) 673, 1123
XceI RCATGY 1 cut(s) 471
XhoI CTCGAG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 946
XspI CTAG 1 cut(s) 773
ZrmI AGTACT 1 cut(s) 81
Zsp2I ATGCAT 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.