AT5G03300

adenosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002092
Physical Location & Seq
Forward (+)
796361 .. 799810
3450 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03300.2

Sequence Viewer

Length: 1038 bp
ATGGCTTCTTCTTCTAACTACGATGGAATCCTTCTCGGTATGGGTAACCCACTCCTCGATATCTCTGCCGTCGTCGACGACGAATTTCTCACCAAATACGACATCAAGTTGAACAATGCGATTCTCGCGGAAGACAAACATTTGCCCATGTATGATGAGATGAGTAGCAAGTTCAATGTTGAATACATTGCCGGAGGTGCTACTCAGAACTCAATCAAAGTGGCTCAGTGGATGCTTCAAATTCCTGGGGCAACCAGTTACATGGGATCCATTGGAAAGGACAAATATGGAGAGGCCATGAAGAAGGATGCTACAGCAGCTGGTGTTAATGTTCACTATTATGAAGATGAGTCGGCACCTACTGGAACTTGTGGTGTCTGTGTTGTTGGTGGAGAAAGGTCTCTTATTGCGAATCTTTCAGCTGCAAACTGCTACAAGGTTGATCACCTTAAGAAGCCTGAAAACTGGGCATTGGTTGAGAAGGCCAAGTTCTACTACATTGCTGGATTCTTCCTCACAGTATCGCCTGAATCCATTCAGTTGGTATCTGAGCACGCTGCTGCAAACAACAAGGTGTTCACAATGAACCTTTCTGCTCCATTCATCTGTGAATTCTTCAAAGATGTGCAGGAGAAGTTCTTGCCATACATGGACTTCGTTTTCGGCAATGAGACAGAGGCAAGAACCTTCTCTAGGGTTCATGGCTGGGAGACTGAAGATGTCGAACAAATAGCCATCAAGATTTCACAGCTTCCCAAGGCCACAGGAACATACAAGAGGACCACTGTGATTACACAGGGCGCTGATCCAGTGGTTGTTGCTGAAGATGGAAAGGTGAAGAAATATCCAGTCATCCCTCTCCCAAAGGAGAAGCTGGTTGACACCAACGGTGCAGGTGATGCATTTGTGGGAGGATTTATGTCACAGTTGGTGAAAGAGAAATCGATTGAGGAATGCGTGAAGGCTGGATGCTATGCGTCGAACGTGGTGATCCAAAGATCTGGCTGCACTTACCCTGAGAAGCCCGACTTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004001 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006166 GO:0006167 GO:0006169 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009167 GO:0009168 GO:0009259 GO:0009260 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019205 GO:0019206 GO:0019438 GO:0019637 GO:0019693 GO:0030054 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0043094 GO:0043101 GO:0043167 GO:0043169 GO:0043174 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046033 GO:0046085 GO:0046086 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0046683 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0080094 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 map00230 map01100
Pfam Domains
Protein Families
PfkB

Protein Analysis

345

Amino Acids

37.85

Weight (kDa)

5.14

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 6 - 286 2.6e-70 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 884
Acc36I ACCTGC 1 cut(s) 884
AccB1I GGYRCC 1 cut(s) 355
AccI GTMKAC 1 cut(s) 75
AccII CGCG 1 cut(s) 128
AciI CCGC 1 cut(s) 128
AclWI GGATC 4 cut(s) 261, 274, 800, 985
AcsI RAATTY 3 cut(s) 83, 240, 611
AcuI CTGAAG 2 cut(s) 735, 843
AfiI CCNNNNNNNGG 1 cut(s) 693
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 5 cut(s) 112, 175, 182, 239, 619
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 4 cut(s) 320, 422, 751, 874
AluI AGCT 4 cut(s) 320, 422, 751, 874
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 3 cut(s) 405, 665, 704
AlwI GGATC 4 cut(s) 261, 274, 800, 985
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 3 cut(s) 294, 483, 759
ApeKI GCWGC 5 cut(s) 317, 422, 557, 560, 1005
ApoI RAATTY 3 cut(s) 83, 240, 611
ArsI GACNNNNNNTTYG 2 cut(s) 644, 676
Asp700I GAANNNNTTC 1 cut(s) 534
AspLEI GCGC 1 cut(s) 803
AspS9I GGNCC 1 cut(s) 780
AsuHPI GGTGA 6 cut(s) 82, 437, 847, 908, 943, 1000
AvaII GGWCC 1 cut(s) 780
BamHI GGATCC 1 cut(s) 266
BanI GGYRCC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 5 cut(s) 329, 409, 544, 547, 992
BccI CCATC 3 cut(s) 17, 743, 821
BceAI ACGGC 1 cut(s) 53
BcgI CGANNNNNNTGC 2 cut(s) 47, 81
BciT130I CCWGG 1 cut(s) 246
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 3 cut(s) 405, 665, 704
BfaI CTAG 1 cut(s) 693
BfmI CTRYAG 1 cut(s) 312
BfoI RGCGCY 1 cut(s) 804
BfrI CTTAAG 1 cut(s) 449
BfuAI ACCTGC 1 cut(s) 884
BglII AGATCT 1 cut(s) 998
BisI GCNGC 5 cut(s) 318, 423, 558, 561, 1006
BlsI GCNGC 5 cut(s) 319, 424, 559, 562, 1007
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 1 cut(s) 780
BmgT120I GGNCC 1 cut(s) 780
BmiI GGNNCC 2 cut(s) 268, 357
BmrFI CCNGG 1 cut(s) 246
BmrI ACTGGG 1 cut(s) 475
BmsI GCATC 4 cut(s) 222, 298, 889, 959
BmuI ACTGGG 1 cut(s) 475
BpiI GAAGAC 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 944
BsaI GGTCTC 1 cut(s) 405
BsaJI CCNNGG 2 cut(s) 245, 756
Bsc4I CCNNNNNNNGG 1 cut(s) 693
Bse1I ACTGG 5 cut(s) 255, 367, 470, 809, 848
Bse3DI GCAATG 3 cut(s) 186, 498, 673
BseBI CCWGG 1 cut(s) 246
BseCI ATCGAT 1 cut(s) 944
BseDI CCNNGG 2 cut(s) 245, 756
BseGI GGATG 4 cut(s) 237, 313, 852, 974
BseLI CCNNNNNNNGG 1 cut(s) 693
BseMI GCAATG 3 cut(s) 186, 498, 673
BseMII CTCAG 4 cut(s) 218, 239, 540, 1008
BseNI ACTGG 5 cut(s) 255, 367, 470, 809, 848
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 5 cut(s) 329, 409, 544, 547, 992
BseYI CCCAGC 1 cut(s) 705
BsgI GTGCAG 3 cut(s) 647, 912, 991
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 3 cut(s) 296, 485, 761
BshNI GGYRCC 1 cut(s) 355
BshVI ATCGAT 1 cut(s) 944
BsiHKAI GWGCWC 1 cut(s) 555
BsiSI CCGG 1 cut(s) 192
BslI CCNNNNNNNGG 1 cut(s) 693
BsmAI GTCTC 3 cut(s) 405, 665, 704
BsmI GAATGC 1 cut(s) 959
BsnI GGCC 3 cut(s) 296, 485, 761
Bso31I GGTCTC 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 555
Bsp143I GATC 5 cut(s) 266, 442, 805, 990, 998
BspACI CCGC 1 cut(s) 128
BspANI GGCC 3 cut(s) 296, 485, 761
BspCNI CTCAG 4 cut(s) 217, 238, 541, 1009
BspDI ATCGAT 1 cut(s) 944
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 2 cut(s) 268, 357
BspMI ACCTGC 1 cut(s) 884
BspPI GGATC 4 cut(s) 261, 274, 800, 985
BspT107I GGYRCC 1 cut(s) 355
BspTI CTTAAG 1 cut(s) 449
BspTNI GGTCTC 1 cut(s) 405
BsrDI GCAATG 3 cut(s) 186, 498, 673
BsrI ACTGG 5 cut(s) 255, 367, 470, 809, 848
BssECI CCNNGG 2 cut(s) 245, 756
BssMI GATC 5 cut(s) 266, 442, 805, 990, 998
BssT1I CCWWGG 1 cut(s) 756
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 4 cut(s) 520, 787, 890, 927
BstAFI CTTAAG 1 cut(s) 449
BstAPI GCANNNNNTGC 1 cut(s) 899
BstC8I GCNNGC 1 cut(s) 555
BstDEI CTNAG 4 cut(s) 204, 225, 549, 1017
BstEII GGTNACC 1 cut(s) 44
BstENI CCTNNNNNAGG 1 cut(s) 691
BstF5I GGATG 4 cut(s) 237, 313, 852, 974
BstFNI CGCG 1 cut(s) 128
BstH2I RGCGCY 1 cut(s) 804
BstHHI GCGC 1 cut(s) 803
BstKTI GATC 5 cut(s) 269, 445, 808, 993, 1001
BstMAI GTCTC 3 cut(s) 405, 665, 704
BstMBI GATC 5 cut(s) 266, 442, 805, 990, 998
BstMWI GCNNNNNNNGC 4 cut(s) 125, 197, 317, 899
BstNI CCWGG 1 cut(s) 246
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 1 cut(s) 312
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 5 cut(s) 329, 409, 544, 547, 992
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 2 cut(s) 266, 998
BstXI CCANNNNNNTGG 3 cut(s) 262, 541, 1001
BstYI RGATCY 2 cut(s) 266, 998
Bsu15I ATCGAT 1 cut(s) 944
BsuRI GGCC 3 cut(s) 296, 485, 761
BsuTUI ATCGAT 1 cut(s) 944
BtsCI GGATG 4 cut(s) 237, 313, 852, 974
BtsIMutI CAGTG 3 cut(s) 233, 783, 816
BveI ACCTGC 1 cut(s) 884
Cac8I GCNNGC 1 cut(s) 555
CaiI CAGNNNCTG 1 cut(s) 320
CfoI GCGC 1 cut(s) 803
Cfr13I GGNCC 1 cut(s) 780
ClaI ATCGAT 1 cut(s) 944
CseI GACGC 1 cut(s) 966
CspCI CAANNNNNGTGG 2 cut(s) 201, 236
CviAII CATG 5 cut(s) 148, 262, 298, 649, 701
DdeI CTNAG 4 cut(s) 204, 225, 549, 1017
DpnI GATC 5 cut(s) 268, 444, 807, 992, 1000
DpnII GATC 5 cut(s) 266, 442, 805, 990, 998
Eco130I CCWWGG 1 cut(s) 756
Eco31I GGTCTC 1 cut(s) 405
Eco32I GATATC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 780
Eco57I CTGAAG 2 cut(s) 735, 843
Eco91I GGTNACC 1 cut(s) 44
EcoNI CCTNNNNNAGG 1 cut(s) 691
EcoO65I GGTNACC 1 cut(s) 44
EcoRI GAATTC 1 cut(s) 611
EcoRII CCWGG 1 cut(s) 244
EcoRV GATATC 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 756
EcoT22I ATGCAT 1 cut(s) 904
ErhI CCWWGG 1 cut(s) 756
FaeI CATG 5 cut(s) 151, 265, 301, 652, 704
FalI AAGNNNNNCTT 1 cut(s) 1013
FatI CATG 5 cut(s) 147, 261, 297, 648, 700
FbaI TGATCA 1 cut(s) 442
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 5 cut(s) 318, 423, 558, 561, 1006
FokI GGATG 4 cut(s) 244, 320, 839, 981
Fsp4HI GCNGC 5 cut(s) 318, 423, 558, 561, 1006
FspBI CTAG 1 cut(s) 693
GlaI GCGC 1 cut(s) 802
GluI GCNGC 5 cut(s) 318, 423, 558, 561, 1006
GsaI CCCAGC 1 cut(s) 709
HaeII RGCGCY 1 cut(s) 804
HaeIII GGCC 3 cut(s) 296, 485, 761
HapII CCGG 1 cut(s) 192
HgaI GACGC 1 cut(s) 966
HhaI GCGC 1 cut(s) 803
Hin1II CATG 5 cut(s) 151, 265, 301, 652, 704
Hin6I GCGC 1 cut(s) 801
HinP1I GCGC 1 cut(s) 801
HincII GTYRAC 2 cut(s) 76, 880
HindII GTYRAC 2 cut(s) 76, 880
HinfI GANTC 6 cut(s) 27, 121, 350, 412, 507, 530
HpaII CCGG 1 cut(s) 192
HphI GGTGA 6 cut(s) 82, 437, 847, 908, 943, 1000
Hpy166II GTNNAC 4 cut(s) 76, 334, 579, 880
Hpy188I TCNGA 2 cut(s) 207, 550
Hpy188III TCNNGA 1 cut(s) 739
Hpy8I GTNNAC 4 cut(s) 76, 334, 579, 880
Hpy99I CGWCG 5 cut(s) 74, 77, 80, 83, 982
HpyAV CCTTC 5 cut(s) 41, 298, 475, 697, 955
HpyCH4III ACNGT 4 cut(s) 520, 787, 890, 927
HpyCH4IV ACGT 1 cut(s) 984
HpyCH4V TGCA 6 cut(s) 425, 563, 628, 893, 902, 1008
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 197, 317, 899
HpyF3I CTNAG 4 cut(s) 204, 225, 549, 1017
HpySE526I ACGT 1 cut(s) 984
Hsp92II CATG 5 cut(s) 151, 265, 301, 652, 704
HspAI GCGC 1 cut(s) 801
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 5 cut(s) 266, 442, 805, 990, 998
LmnI GCTCC 1 cut(s) 601
Lsp1109I GCAGC 5 cut(s) 329, 409, 544, 547, 992
LweI GCATC 4 cut(s) 222, 298, 889, 959
MaeI CTAG 1 cut(s) 693
MaeII ACGT 1 cut(s) 984
MaeIII GTNAC 3 cut(s) 44, 257, 921
MalI GATC 5 cut(s) 268, 444, 807, 992, 1000
MboI GATC 5 cut(s) 266, 442, 805, 990, 998
MboII GAAGA 9 cut(s) 3, 143, 313, 356, 502, 607, 728, 836, 850
MflI RGATCY 2 cut(s) 266, 998
MhlI GDGCHC 1 cut(s) 555
MluCI AATT 3 cut(s) 83, 240, 611
MlyI GAGTC 1 cut(s) 359
MnlI CCTC 9 cut(s) 65, 188, 286, 524, 670, 771, 867, 905, 943
Mph1103I ATGCAT 1 cut(s) 904
MroXI GAANNNNTTC 1 cut(s) 534
MseI TTAA 3 cut(s) 327, 450, 1032
MslI CAYNNNNRTG 1 cut(s) 339
MspA1I CMGCKG 2 cut(s) 320, 422
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 1 cut(s) 246
Mva1269I GAATGC 1 cut(s) 959
MvaI CCWGG 1 cut(s) 246
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 4 cut(s) 125, 197, 317, 899
NdeII GATC 5 cut(s) 266, 442, 805, 990, 998
NlaIII CATG 5 cut(s) 151, 265, 301, 652, 704
NlaIV GGNNCC 2 cut(s) 268, 357
NmuCI GTSAC 1 cut(s) 921
NsiI ATGCAT 1 cut(s) 904
PaqCI CACCTGC 1 cut(s) 884
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PctI GAATGC 1 cut(s) 959
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 5 cut(s) 27, 121, 412, 507, 530
PkrI GCNGC 5 cut(s) 319, 424, 559, 562, 1007
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 244
PspEI GGTNACC 1 cut(s) 44
PspFI CCCAGC 1 cut(s) 705
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 2 cut(s) 268, 357
PspPI GGNCC 1 cut(s) 780
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 2 cut(s) 266, 998
PvuII CAGCTG 2 cut(s) 320, 422
RseI CAYNNNNRTG 1 cut(s) 339
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 3 cut(s) 327, 450, 1032
SatI GCNGC 5 cut(s) 318, 423, 558, 561, 1006
Sau3AI GATC 5 cut(s) 266, 442, 805, 990, 998
Sau96I GGNCC 1 cut(s) 780
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 1 cut(s) 555
SfaNI GCATC 4 cut(s) 222, 298, 889, 959
SfcI CTRYAG 1 cut(s) 312
SgrDI CGTCGACG 1 cut(s) 74
SinI GGWCC 1 cut(s) 780
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 3 cut(s) 83, 240, 611
SsiI CCGC 1 cut(s) 128
SspMI CTAG 1 cut(s) 693
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 4 cut(s) 520, 787, 890, 927
TaiI ACGT 1 cut(s) 987
TaqI TCGA 5 cut(s) 57, 75, 723, 944, 980
TasI AATT 3 cut(s) 83, 240, 611
TfiI GAWTC 5 cut(s) 27, 121, 412, 507, 530
Tru1I TTAA 3 cut(s) 327, 450, 1032
Tru9I TTAA 3 cut(s) 327, 450, 1032
TscAI CASTG 3 cut(s) 233, 790, 816
TseFI GTSAC 1 cut(s) 921
TseI GCWGC 5 cut(s) 317, 422, 557, 560, 1005
Tsp45I GTSAC 1 cut(s) 921
TspDTI ATGAA 5 cut(s) 314, 357, 592, 599, 689
TspRI CASTG 3 cut(s) 233, 790, 816
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 780
XagI CCTNNNNNAGG 1 cut(s) 691
XapI RAATTY 3 cut(s) 83, 240, 611
XmiI GTMKAC 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 1 cut(s) 693
Zsp2I ATGCAT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.