MD12G1116000.v1.1

adenosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002092
Physical Location & Seq
Reverse (-)
18470696 .. 18473776
3081 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1116000.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGCAGCTCTCTCTCTCAATGGCGTCCGAGGGAATTCTGTTGGGGATGGGAAACCCACTGCTAGACATCTCCGCCGTCGTCGACGAGGAGTTCTTGCAGAAATATGACCTCAAGCCCAACAATGCGATTCTTGCAGAGGACAAGCACCTGCCCATGTACGACGAAATGTCTTCCAAGTACAACGTGGAGTACATTGCTGGAGGTGCTACTCAGAATTCCATTCGAGTTGCTCAGTGGATGCTTCAAGTACCTGGTGCAACAAGTTATATGGGTTCCATTGGAAAGGACAAGTATGGTGAAGAGATGAAGAAGAACTCAAAACTTGCTGGTGTTAATGTTCACTATTATGTGGATGAGACTGCACCAACGGGAACTTGTGCTGTCTGTGTTGTTGGTGGTGAAAGGTCCCTTATTGCCAACTTGTCAGCCGCTAACTGCTACAAATTTGAGCATTTAAAGAAACCCGAAAATTGGGCACTGGTTGAGAAGGCCAAATACATCTACATTGCTGGTTTTTTTCTAACTGTATCCCCAGACTCCATCCAGCTTGTTGCTGAACACGCTGCTGCAAATAACAAGGTTTTTTCAATGAACCTTTCTGCTCCATTTATCTGTGAGTTTTTCAAGGATGCTCAGGATAAAGCTTTACCGTATGTGGACTATGTTTTTGGAAATGAGACGGAAGCAAGAACCTTCTCGAAAGTTCATGGCTGGGAGATTGATGATGTCGAGCAAATAGCTCTAAAGATCTCCCAATGGCCCAAAGCGTCAGGGACACACAAGAGGATTACTGTTATTACTCAGGGCGCAGATCCCGTGGTTGTTGCTGAGGATGGCAAAGTGAAAAAGTTCCCAGTGGACAAGTTGCCCAAAGAGAAATTGGTTGATACCAATGGAGCGGGGGATGCATTCGTTGGAGGTTTTCTATCTCAATTGGTTCAAGAGAAAGCCATTGAAGACTGCGTGAAAGCTGGCAACTATGCTGCAAATGTTGTCATCCAGAGGTCTGGCTGCACCTACCCGGAGAAGCCTGATTTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004001 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006166 GO:0006167 GO:0006169 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009167 GO:0009168 GO:0009259 GO:0009260 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019205 GO:0019206 GO:0019438 GO:0019637 GO:0019693 GO:0030054 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0043094 GO:0043101 GO:0043167 GO:0043169 GO:0043174 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046033 GO:0046085 GO:0046086 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0046683 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0080094 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00230 ko01100 map00230 map01100
Pfam Domains
Protein Families
PfkB

Protein Analysis

348

Amino Acids

38.09

Weight (kDa)

5.3

Isoelectric Point (pI)

30.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 32 - 341 2.7e-75 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 156
Acc36I ACCTGC 1 cut(s) 156
AccBSI CCGCTC 1 cut(s) 899
AccI GTMKAC 1 cut(s) 81
AciI CCGC 3 cut(s) 72, 429, 899
AclWI GGATC 1 cut(s) 806
AcsI RAATTY 3 cut(s) 33, 214, 443
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 4 cut(s) 158, 179, 191, 249
AfiI CCNNNNNNNGG 1 cut(s) 471
AgsI TTSAA 6 cut(s) 245, 588, 625, 941, 956, 1039
AhdI GACNNNNNGTC 1 cut(s) 166
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 5 cut(s) 7, 547, 644, 740, 971
AluI AGCT 5 cut(s) 7, 547, 644, 740, 971
Alw26I GTCTC 2 cut(s) 350, 671
AlwI GGATC 1 cut(s) 806
AoxI GGCC 2 cut(s) 489, 758
ApeKI GCWGC 5 cut(s) 4, 563, 566, 983, 1011
ApoI RAATTY 3 cut(s) 33, 214, 443
ArsI GACNNNNNNTTYG 2 cut(s) 650, 682
Asp700I GAANNNNTTC 1 cut(s) 848
AspLEI GCGC 1 cut(s) 809
AspS9I GGNCC 2 cut(s) 405, 759
AsuC2I CCSGG 1 cut(s) 1022
AsuHPI GGTGA 2 cut(s) 308, 410
AvaII GGWCC 1 cut(s) 405
BaeGI GKGCMC 1 cut(s) 478
BarI GAAGNNNNNNTAC 2 cut(s) 479, 511
BbsI GAAGAC 2 cut(s) 162, 963
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 5 cut(s) 16, 550, 553, 970, 998
BccI CCATC 3 cut(s) 40, 548, 827
BceAI ACGGC 1 cut(s) 59
BciT130I CCWGG 1 cut(s) 252
BciVI GTATCC 1 cut(s) 538
BcnI CCSGG 1 cut(s) 1022
BcoDI GTCTC 2 cut(s) 350, 671
BfaI CTAG 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 156
BfuI GTATCC 1 cut(s) 538
BglII AGATCT 1 cut(s) 747
BisI GCNGC 6 cut(s) 5, 429, 564, 567, 984, 1012
BlsI GCNGC 6 cut(s) 6, 430, 565, 568, 985, 1013
Bme1390I CCNGG 2 cut(s) 252, 1022
Bme18I GGWCC 1 cut(s) 405
BmeRI GACNNNNNGTC 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 405, 759
BmiI GGNNCC 2 cut(s) 274, 407
BmrFI CCNGG 2 cut(s) 252, 1022
BmrI ACTGGG 1 cut(s) 848
BmsI GCATC 3 cut(s) 228, 619, 895
BmuI ACTGGG 1 cut(s) 848
BpiI GAAGAC 2 cut(s) 162, 963
BpmI CTGGAG 1 cut(s) 219
Bpu10I CCTNAGC 2 cut(s) 633, 828
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 1022
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 2 cut(s) 27, 816
Bsc4I CCNNNNNNNGG 1 cut(s) 471
Bse1I ACTGG 2 cut(s) 483, 854
Bse3DI GCAATG 2 cut(s) 192, 504
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 27, 816
BseGI GGATG 8 cut(s) 51, 243, 358, 540, 634, 838, 910, 996
BseLI CCNNNNNNNGG 1 cut(s) 471
BseMI GCAATG 2 cut(s) 192, 504
BseMII CTCAG 5 cut(s) 224, 245, 647, 815, 819
BseNI ACTGG 2 cut(s) 483, 854
BseRI GAGGAG 1 cut(s) 101
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 5 cut(s) 16, 550, 553, 970, 998
BseYI CCCAGC 1 cut(s) 711
BsgI GTGCAG 2 cut(s) 345, 997
BshFI GGCC 2 cut(s) 491, 760
BsiSI CCGG 1 cut(s) 1022
BslFI GGGAC 2 cut(s) 391, 787
BslI CCNNNNNNNGG 1 cut(s) 471
BsmAI GTCTC 2 cut(s) 350, 671
BsmBI CGTCTC 1 cut(s) 671
BsmFI GGGAC 2 cut(s) 391, 787
BsmI GAATGC 1 cut(s) 908
BsnI GGCC 2 cut(s) 491, 760
Bsp1286I GDGCHC 1 cut(s) 478
Bsp143I GATC 2 cut(s) 747, 811
BspACI CCGC 3 cut(s) 72, 429, 899
BspANI GGCC 2 cut(s) 491, 760
BspCNI CTCAG 5 cut(s) 223, 244, 646, 814, 820
BspLI GGNNCC 2 cut(s) 274, 407
BspMI ACCTGC 1 cut(s) 156
BspPI GGATC 1 cut(s) 806
BsrBI CCGCTC 1 cut(s) 899
BsrDI GCAATG 2 cut(s) 192, 504
BsrI ACTGG 2 cut(s) 483, 854
BssECI CCNNGG 2 cut(s) 27, 816
BssMI GATC 2 cut(s) 747, 811
BssNI GRCGYC 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 526, 651, 793
Bst6I CTCTTC 1 cut(s) 294
BstACI GRCGYC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 973
BstDEI CTNAG 5 cut(s) 210, 231, 633, 801, 828
BstDSI CCRYGG 1 cut(s) 816
BstF5I GGATG 8 cut(s) 51, 243, 358, 540, 634, 838, 910, 996
BstHHI GCGC 1 cut(s) 809
BstKTI GATC 2 cut(s) 750, 814
BstMAI GTCTC 2 cut(s) 350, 671
BstMBI GATC 2 cut(s) 747, 811
BstMWI GCNNNNNNNGC 4 cut(s) 131, 203, 560, 905
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 2 cut(s) 250, 1020
BstSLI GKGCMC 1 cut(s) 478
BstV1I GCAGC 5 cut(s) 16, 550, 553, 970, 998
BstV2I GAAGAC 2 cut(s) 162, 963
BstX2I RGATCY 2 cut(s) 747, 811
BstXI CCANNNNNNTGG 1 cut(s) 1007
BstYI RGATCY 2 cut(s) 747, 811
BsuI GTATCC 1 cut(s) 538
BsuRI GGCC 2 cut(s) 491, 760
BtgI CCRYGG 1 cut(s) 816
BtsCI GGATG 8 cut(s) 51, 243, 358, 540, 634, 838, 910, 996
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 4 cut(s) 56, 239, 476, 861
BveI ACCTGC 1 cut(s) 156
Cac8I GCNNGC 1 cut(s) 973
CfoI GCGC 1 cut(s) 809
Cfr13I GGNCC 2 cut(s) 405, 759
CseI GACGC 2 cut(s) 12, 756
CsiI ACCWGGT 1 cut(s) 250
Csp6I GTAC 4 cut(s) 157, 178, 190, 248
CviAII CATG 2 cut(s) 154, 707
CviQI GTAC 4 cut(s) 157, 178, 190, 248
DdeI CTNAG 5 cut(s) 210, 231, 633, 801, 828
DpnI GATC 2 cut(s) 749, 813
DpnII GATC 2 cut(s) 747, 811
DraI TTTAAA 1 cut(s) 456
DriI GACNNNNNGTC 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 294
Eam1105I GACNNNNNGTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 294
EciI GGCGGA 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 405
EcoO109I RGGNCCY 1 cut(s) 405
EcoRI GAATTC 2 cut(s) 33, 214
EcoRII CCWGG 1 cut(s) 250
EcoT22I ATGCAT 1 cut(s) 910
Esp3I CGTCTC 1 cut(s) 671
FaeI CATG 2 cut(s) 157, 710
FaqI GGGAC 2 cut(s) 391, 787
FatI CATG 2 cut(s) 153, 706
FauI CCCGC 1 cut(s) 892
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 6 cut(s) 5, 429, 564, 567, 984, 1012
FokI GGATG 8 cut(s) 58, 250, 365, 527, 641, 845, 917, 983
Fsp4HI GCNGC 6 cut(s) 5, 429, 564, 567, 984, 1012
FspBI CTAG 1 cut(s) 62
GlaI GCGC 1 cut(s) 808
GluI GCNGC 6 cut(s) 5, 429, 564, 567, 984, 1012
GsaI CCCAGC 1 cut(s) 715
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 2 cut(s) 491, 760
HapII CCGG 1 cut(s) 1022
HgaI GACGC 2 cut(s) 12, 756
HhaI GCGC 1 cut(s) 809
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 2 cut(s) 157, 710
Hin6I GCGC 1 cut(s) 807
HinP1I GCGC 1 cut(s) 807
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 642
HinfI GANTC 2 cut(s) 127, 536
HpaII CCGG 1 cut(s) 1022
HphI GGTGA 2 cut(s) 308, 410
Hpy166II GTNNAC 4 cut(s) 82, 340, 658, 859
Hpy188I TCNGA 2 cut(s) 28, 213
Hpy188III TCNNGA 4 cut(s) 635, 697, 941, 1000
Hpy8I GTNNAC 4 cut(s) 82, 340, 658, 859
Hpy99I CGWCG 4 cut(s) 80, 83, 86, 164
HpyAV CCTTC 2 cut(s) 481, 703
HpyCH4III ACNGT 3 cut(s) 526, 651, 793
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 9 cut(s) 4, 97, 134, 257, 362, 569, 908, 986, 1014
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 203, 560, 905
HpyF3I CTNAG 5 cut(s) 210, 231, 633, 801, 828
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 2 cut(s) 157, 710
HspAI GCGC 1 cut(s) 807
Kzo9I GATC 2 cut(s) 747, 811
LmnI GCTCC 2 cut(s) 607, 896
Lsp1109I GCAGC 5 cut(s) 16, 550, 553, 970, 998
LweI GCATC 3 cut(s) 228, 619, 895
MabI ACCWGGT 1 cut(s) 250
MaeI CTAG 1 cut(s) 62
MaeII ACGT 1 cut(s) 183
MalI GATC 2 cut(s) 749, 813
MbiI CCGCTC 1 cut(s) 899
MboI GATC 2 cut(s) 747, 811
MboII GAAGA 5 cut(s) 162, 311, 319, 322, 968
MfeI CAATTG 1 cut(s) 932
MflI RGATCY 2 cut(s) 747, 811
MhlI GDGCHC 1 cut(s) 478
MluCI AATT 7 cut(s) 33, 214, 443, 469, 878, 932, 1039
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 895
MnlI CCTC 9 cut(s) 22, 79, 119, 130, 194, 777, 823, 911, 996
Mph1103I ATGCAT 1 cut(s) 910
MroXI GAANNNNTTC 1 cut(s) 848
MseI TTAA 3 cut(s) 333, 455, 1042
MslI CAYNNNNRTG 1 cut(s) 345
MspI CCGG 1 cut(s) 1022
MspR9I CCNGG 2 cut(s) 252, 1022
MunI CAATTG 1 cut(s) 932
Mva1269I GAATGC 1 cut(s) 908
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 4 cut(s) 131, 203, 560, 905
NciI CCSGG 1 cut(s) 1022
NdeII GATC 2 cut(s) 747, 811
NlaIII CATG 2 cut(s) 157, 710
NlaIV GGNNCC 2 cut(s) 274, 407
NsiI ATGCAT 1 cut(s) 910
PaqCI CACCTGC 1 cut(s) 156
PctI GAATGC 1 cut(s) 908
PdmI GAANNNNTTC 1 cut(s) 848
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 6 cut(s) 6, 430, 565, 568, 985, 1013
PleI GAGTC 1 cut(s) 530
PpsI GAGTC 1 cut(s) 530
PpuMI RGGWCCY 1 cut(s) 405
Psp5II RGGWCCY 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 250
PspFI CCCAGC 1 cut(s) 711
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 2 cut(s) 274, 407
PspPI GGNCC 2 cut(s) 405, 759
PspPPI RGGWCCY 1 cut(s) 405
PsuI RGATCY 2 cut(s) 747, 811
RsaI GTAC 4 cut(s) 158, 179, 191, 249
RsaNI GTAC 4 cut(s) 157, 178, 190, 248
RseI CAYNNNNRTG 1 cut(s) 345
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 3 cut(s) 333, 455, 1042
SatI GCNGC 6 cut(s) 5, 429, 564, 567, 984, 1012
Sau3AI GATC 2 cut(s) 747, 811
Sau96I GGNCC 2 cut(s) 405, 759
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 2 cut(s) 252, 1022
SduI GDGCHC 1 cut(s) 478
SexAI ACCWGGT 1 cut(s) 250
SfaNI GCATC 3 cut(s) 228, 619, 895
SgrDI CGTCGACG 1 cut(s) 80
SinI GGWCC 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 7 cut(s) 33, 214, 443, 469, 878, 932, 1039
SsiI CCGC 3 cut(s) 72, 429, 899
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 2 cut(s) 250, 1020
TaaI ACNGT 3 cut(s) 526, 651, 793
TaiI ACGT 1 cut(s) 186
TaqI TCGA 4 cut(s) 81, 223, 698, 729
TasI AATT 7 cut(s) 33, 214, 443, 469, 878, 932, 1039
TatI WGTACW 2 cut(s) 177, 189
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 3 cut(s) 333, 455, 1042
Tru9I TTAA 3 cut(s) 333, 455, 1042
TscAI CASTG 4 cut(s) 63, 239, 483, 861
TseI GCWGC 5 cut(s) 4, 563, 566, 983, 1011
TspDTI ATGAA 3 cut(s) 320, 605, 695
TspGWI ACGGA 1 cut(s) 695
TspRI CASTG 4 cut(s) 63, 239, 483, 861
VpaK11BI GGWCC 1 cut(s) 405
XapI RAATTY 3 cut(s) 33, 214, 443
XcmI CCANNNNNNNNNTGG 2 cut(s) 181, 877
XmiI GTMKAC 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 848
XspI CTAG 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.