Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002092

Physical Location & Seq

Genomic Coordinates Reverse (-)

21935183 .. 21938800

Gene Structure 3618 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1106200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGGCACTCGAGCAGATCCTCTTGGGGATGGGCAATCCTCTTCTCGACATATCTGCCGTCGTCGACGAAGAATTCTTGACAAAATATGACATCAAGCCGAACAATGCCATTCTTGCTGAGGACAAGCATCTGCCCATGTACGACGAAATGTCTTCCAACTACAATGTAGAGTATATTGCTGGAGGTGCTACTCAAAATTCAATCAAAGTTGCCCAGTGGATGCTTCAAGTTCCTGGCGCAACTAGTTACATTGGATCTATCGGAAAGGACAAGTATGGTGAGGAGATGAAGAAAAATTGTAGACTTGCTGGTGTTAATGTGCAATATTATGAAGATGACAAAGCTCCAACAGGAACATGTGCTGTTTGTGTAGTTGGTGGTGAAAGGTCTCTTGTTGCTAACCTATCAGCTGCAAATTGTTACAAAACTGAGCATTTGAAGCAACCTGAAAATTGGGCCCTGGTTGAGAAGGCAAAATATTACTATATTGCTGGGTTTTTCCTTACCGTGTCTCCTGAATCCATTCAACTGGTAGCTGAACATGCAGCTGCAAACAATAAGGTATTCACGATGAATCTTTCCGCTCCTTTCATTTGTGAGTTCTTCAAGGATGTACAGGAGAAAGCACTTCCGTACATGGACTATGTTTTTGGGAATGAGACCGAAGCAAGAACCTTTTCAAGGGTTCATGGCTGGAAGACTGAGAATGTCGAGGAGATCGCCATAAAGATTTCCCAGTTGCCCAAAGCATCAGGTACGCACAAGAGGATTACTGTGATCACACAAGGTGCAGATCCTGTTGTAGTTGCTGAGGATGGGAAGGTGAAACTATTTCCTGTCATATTGTTACCTAAGGAGAAGCTGGTTGATACCAATGGAGCCGGAGATGCATTTGTTGGGGGATTTTTGTCACAGCTGGTTCAAGAGAAGCCCATTGAGGACTGTGTTAAAGCTGGCTGTTATGCAGCAAACGTTGTCATCCAAAGGTCAGGCTGCACATACCCAGAAAAGCCCGATTTCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004001 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006166 GO:0006167 GO:0006169 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009167 GO:0009168 GO:0009259 GO:0009260 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019205 GO:0019206 GO:0019438 GO:0019637 GO:0019693 GO:0034404 GO:0034641 GO:0034654 GO:0042278 GO:0042451 GO:0042455 GO:0043094 GO:0043101 GO:0043167 GO:0043169 GO:0043174 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046033 GO:0046085 GO:0046086 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0046872 GO:0046914 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00230 ko01100 map00230 map01100

Pfam Domains

Protein Families

PfkB

Protein Analysis

342

Amino Acids

37.57

Weight (kDa)

5.22

Isoelectric Point (pI)

36.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	25 - 335	1.5e-74	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002092)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09820 AT3G09820 AT5G03300 AT5G03300 AT5G03300
fragaria_vesca	FvH4_6g16080 FvH4_6g16090 FvH4_6g16090 FvH4_7g19110
malus_domestica	MD01G1106200.v1.1 MD07G1172600.v1.1 MD12G1116000.v1.1
prunus_persica	Prupe.2G211700_v2.0.a1 Prupe.6G235100_v2.0.a1
pyrus_communis	pycom01g13290 pycom04g09090 pycom07g16710 pycom12g11550 pycom14g07710
rosa_chinensis	RchiOBHm_Chr1g0363001 RchiOBHm_Chr3g0469911 RchiOBHm_Chr3g0469921
rosa_laevigata	RLG00000024312 RLG00000024313 RLG00000027603
rosa_multiflora	Rmu_co8309403.1_g000001 Rmu_sc0000174.1_g000004 Rmu_sc0008730.1_g000002
rosa_roxburghii	Rroxscaffold_4G00293560 Rroxscaffold_6G00411370
rosa_rugosa	Rorug01G0303400 Rorug03G0105500 Rorug03G0105600
rosa_samantha	Rh1AG312400 Rh1CG292600 Rh1DG306400 Rh3AG154400 Rh3BG178500 Rh3CG169100 Rh3DG196600 Rh3DG196700
rosa_wichuraiana	Rw1G027690 Rw3G014540 Rw3G014550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	584
AccI	GTMKAC	2 cut(s)	63, 301
AciI	CCGC	1 cut(s)	582
AclI	AACGTT	1 cut(s)	972
AclWI	GGATC	3 cut(s)	10, 262, 788
AcsI	RAATTY	2 cut(s)	71, 196
AdeI	CACNNNGTG	1 cut(s)	788
AfaI	GTAC	4 cut(s)	140, 615, 635, 757
AfiI	CCNNNNNNNGG	1 cut(s)	681
AflIII	ACRYGT	1 cut(s)	356
AgsI	TTSAA	7 cut(s)	201, 227, 439, 527, 607, 681, 923
AhdI	GACNNNNNGTC	1 cut(s)	148
AhlI	ACTAGT	1 cut(s)	242
AjnI	CCWGG	2 cut(s)	232, 459
AluBI	AGCT	7 cut(s)	344, 410, 536, 548, 862, 916, 953
AluI	AGCT	7 cut(s)	344, 410, 536, 548, 862, 916, 953
Alw26I	GTCTC	3 cut(s)	393, 516, 653
AlwI	GGATC	3 cut(s)	10, 262, 788
AlwNI	CAGNNNCTG	1 cut(s)	797
Ama87I	CYCGRG	1 cut(s)	8
AoxI	GGCC	1 cut(s)	456
ApaI	GGGCCC	1 cut(s)	460
ApeKI	GCWGC	5 cut(s)	410, 545, 548, 965, 993
ApoI	RAATTY	2 cut(s)	71, 196
ArsI	GACNNNNNNTTYG	2 cut(s)	632, 664
Asp700I	GAANNNNTTC	2 cut(s)	522, 676
AspLEI	GCGC	1 cut(s)	239
AspS9I	GGNCC	2 cut(s)	456, 457
AsuHPI	GGTGA	3 cut(s)	290, 392, 835
AvaI	CYCGRG	1 cut(s)	8
AxyI	CCTNAGG	1 cut(s)	852
BaeGI	GKGCMC	1 cut(s)	460
BanII	GRGCYC	1 cut(s)	460
BbsI	GAAGAC	2 cut(s)	144, 704
BbvCI	CCTCAGC	2 cut(s)	117, 810
BbvI	GCAGC	5 cut(s)	397, 535, 557, 977, 980
BccI	CCATC	2 cut(s)	22, 809
BceAI	ACGGC	1 cut(s)	41
BcgI	CGANNNNNNTGC	2 cut(s)	35, 69
BciT130I	CCWGG	2 cut(s)	234, 461
BclI	TGATCA	1 cut(s)	777
BcoDI	GTCTC	3 cut(s)	393, 516, 653
BcuI	ACTAGT	1 cut(s)	242
BfaI	CTAG	1 cut(s)	243
BisI	GCNGC	5 cut(s)	411, 546, 549, 966, 994
BlsI	GCNGC	5 cut(s)	412, 547, 550, 967, 995
Bme1390I	CCNGG	2 cut(s)	234, 461
BmeRI	GACNNNNNGTC	1 cut(s)	148
BmeT110I	CYCGRG	1 cut(s)	8
BmgT120I	GGNCC	2 cut(s)	456, 457
BmiI	GGNNCC	2 cut(s)	458, 880
BmrFI	CCNGG	2 cut(s)	234, 461
BmrI	ACTGGG	2 cut(s)	208, 730
BmsI	GCATC	4 cut(s)	136, 210, 758, 877
BmuI	ACTGGG	2 cut(s)	208, 730
BpiI	GAAGAC	2 cut(s)	144, 704
BpmI	CTGGAG	1 cut(s)	201
Bpu10I	CCTNAGC	2 cut(s)	117, 810
BsaI	GGTCTC	2 cut(s)	393, 653
BsaJI	CCNNGG	1 cut(s)	459
BsaXI	ACNNNNNCTCC	2 cut(s)	496, 526
Bsc4I	CCNNNNNNNGG	1 cut(s)	681
Bse1I	ACTGG	3 cut(s)	214, 534, 736
Bse21I	CCTNAGG	1 cut(s)	852
BseBI	CCWGG	2 cut(s)	234, 461
BseDI	CCNNGG	1 cut(s)	459
BseGI	GGATG	5 cut(s)	33, 225, 616, 820, 978
BseLI	CCNNNNNNNGG	1 cut(s)	681
BseMII	CTCAG	4 cut(s)	108, 420, 693, 801
BseNI	ACTGG	3 cut(s)	214, 534, 736
BseRI	GAGGAG	2 cut(s)	296, 728
BseSI	GKGCMC	1 cut(s)	460
BseXI	GCAGC	5 cut(s)	397, 535, 557, 977, 980
BseYI	CCCAGC	1 cut(s)	491
BsgI	GTGCAG	2 cut(s)	810, 979
BshFI	GGCC	1 cut(s)	458
BsiHKCI	CYCGRG	1 cut(s)	8
BsiSI	CCGG	1 cut(s)	882
BslI	CCNNNNNNNGG	1 cut(s)	681
BsmAI	GTCTC	3 cut(s)	393, 516, 653
BsnI	GGCC	1 cut(s)	458
Bso31I	GGTCTC	2 cut(s)	393, 653
BsoBI	CYCGRG	1 cut(s)	8
Bsp120I	GGGCCC	1 cut(s)	456
Bsp1286I	GDGCHC	1 cut(s)	460
Bsp1407I	TGTACA	1 cut(s)	613
Bsp143I	GATC	5 cut(s)	15, 254, 717, 777, 793
BspACI	CCGC	1 cut(s)	582
BspANI	GGCC	1 cut(s)	458
BspCNI	CTCAG	4 cut(s)	109, 421, 694, 802
BspLI	GGNNCC	2 cut(s)	458, 880
BspPI	GGATC	3 cut(s)	10, 262, 788
BspTNI	GGTCTC	2 cut(s)	393, 653
BsrBI	CCGCTC	1 cut(s)	584
BsrGI	TGTACA	1 cut(s)	613
BsrI	ACTGG	3 cut(s)	214, 534, 736
BssECI	CCNNGG	1 cut(s)	459
BssMI	GATC	5 cut(s)	15, 254, 717, 777, 793
Bst2UI	CCWGG	2 cut(s)	234, 461
Bst4CI	ACNGT	3 cut(s)	508, 775, 944
Bst6I	CTCTTC	1 cut(s)	45
BstAUI	TGTACA	1 cut(s)	613
BstC8I	GCNNGC	1 cut(s)	955
BstDEI	CTNAG	5 cut(s)	117, 429, 702, 810, 852
BstENI	CCTNNNNNAGG	1 cut(s)	679
BstF5I	GGATG	5 cut(s)	33, 225, 616, 820, 978
BstHHI	GCGC	1 cut(s)	239
BstKTI	GATC	5 cut(s)	18, 257, 720, 780, 796
BstMAI	GTCTC	3 cut(s)	393, 516, 653
BstMBI	GATC	5 cut(s)	15, 254, 717, 777, 793
BstMWI	GCNNNNNNNGC	5 cut(s)	113, 185, 439, 542, 887
BstNI	CCWGG	2 cut(s)	234, 461
BstNSI	RCATGY	2 cut(s)	360, 545
BstSCI	CCNGG	2 cut(s)	232, 459
BstSLI	GKGCMC	1 cut(s)	460
BstV1I	GCAGC	5 cut(s)	397, 535, 557, 977, 980
BstV2I	GAAGAC	2 cut(s)	144, 704
BstX2I	RGATCY	3 cut(s)	15, 254, 793
BstXI	CCANNNNNNTGG	1 cut(s)	529
BstYI	RGATCY	3 cut(s)	15, 254, 793
Bsu36I	CCTNAGG	1 cut(s)	852
BsuRI	GGCC	1 cut(s)	458
BtsCI	GGATG	5 cut(s)	33, 225, 616, 820, 978
BtsIMutI	CAGTG	1 cut(s)	221
Cac8I	GCNNGC	1 cut(s)	955
CaiI	CAGNNNCTG	1 cut(s)	797
CfoI	GCGC	1 cut(s)	239
Cfr13I	GGNCC	2 cut(s)	456, 457
Csp6I	GTAC	4 cut(s)	139, 614, 634, 756
CviAII	CATG	5 cut(s)	136, 357, 542, 637, 689
CviQI	GTAC	4 cut(s)	139, 614, 634, 756
DdeI	CTNAG	5 cut(s)	117, 429, 702, 810, 852
DpnI	GATC	5 cut(s)	17, 256, 719, 779, 795
DpnII	GATC	5 cut(s)	15, 254, 717, 777, 793
DraIII	CACNNNGTG	1 cut(s)	788
DriI	GACNNNNNGTC	1 cut(s)	148
Eam1104I	CTCTTC	1 cut(s)	45
Eam1105I	GACNNNNNGTC	1 cut(s)	148
EarI	CTCTTC	1 cut(s)	45
Eco24I	GRGCYC	1 cut(s)	460
Eco31I	GGTCTC	2 cut(s)	393, 653
Eco81I	CCTNAGG	1 cut(s)	852
Eco88I	CYCGRG	1 cut(s)	8
EcoNI	CCTNNNNNAGG	1 cut(s)	679
EcoO109I	RGGNCCY	1 cut(s)	457
EcoRI	GAATTC	1 cut(s)	71
EcoRII	CCWGG	2 cut(s)	232, 459
EcoT22I	ATGCAT	1 cut(s)	892
EcoT38I	GRGCYC	1 cut(s)	460
FaeI	CATG	5 cut(s)	139, 360, 545, 640, 692
FatI	CATG	5 cut(s)	135, 356, 541, 636, 688
FbaI	TGATCA	1 cut(s)	777
FblI	GTMKAC	2 cut(s)	63, 301
Fnu4HI	GCNGC	5 cut(s)	411, 546, 549, 966, 994
FokI	GGATG	5 cut(s)	40, 232, 623, 827, 965
FriOI	GRGCYC	1 cut(s)	460
Fsp4HI	GCNGC	5 cut(s)	411, 546, 549, 966, 994
FspBI	CTAG	1 cut(s)	243
GlaI	GCGC	1 cut(s)	238
GluI	GCNGC	5 cut(s)	411, 546, 549, 966, 994
GsaI	CCCAGC	1 cut(s)	495
GsuI	CTGGAG	1 cut(s)	201
HaeIII	GGCC	1 cut(s)	458
HapII	CCGG	1 cut(s)	882
HhaI	GCGC	1 cut(s)	239
Hin1II	CATG	5 cut(s)	139, 360, 545, 640, 692
Hin6I	GCGC	1 cut(s)	237
HinP1I	GCGC	1 cut(s)	237
HincII	GTYRAC	1 cut(s)	64
HindII	GTYRAC	1 cut(s)	64
HinfI	GANTC	2 cut(s)	518, 574
HpaII	CCGG	1 cut(s)	882
HphI	GGTGA	3 cut(s)	290, 392, 835
Hpy166II	GTNNAC	2 cut(s)	64, 302
Hpy188I	TCNGA	1 cut(s)	263
Hpy188III	TCNNGA	5 cut(s)	44, 76, 515, 568, 923
Hpy8I	GTNNAC	2 cut(s)	64, 302
Hpy99I	CGWCG	4 cut(s)	62, 65, 68, 146
HpyAV	CCTTC	2 cut(s)	463, 814
HpyCH4III	ACNGT	3 cut(s)	508, 775, 944
HpyCH4IV	ACGT	1 cut(s)	972
HpyCH4V	TGCA	8 cut(s)	322, 413, 545, 551, 791, 890, 965, 996
HpyF10VI	GCNNNNNNNGC	5 cut(s)	113, 185, 439, 542, 887
HpyF3I	CTNAG	5 cut(s)	117, 429, 702, 810, 852
HpySE526I	ACGT	1 cut(s)	972
Hsp92II	CATG	5 cut(s)	139, 360, 545, 640, 692
HspAI	GCGC	1 cut(s)	237
Ksp22I	TGATCA	1 cut(s)	777
Kzo9I	GATC	5 cut(s)	15, 254, 717, 777, 793
LmnI	GCTCC	3 cut(s)	349, 589, 878
Lsp1109I	GCAGC	5 cut(s)	397, 535, 557, 977, 980
LweI	GCATC	4 cut(s)	136, 210, 758, 877
MaeI	CTAG	1 cut(s)	243
MaeII	ACGT	1 cut(s)	972
MaeIII	GTNAC	4 cut(s)	245, 419, 846, 909
MalI	GATC	5 cut(s)	17, 256, 719, 779, 795
MbiI	CCGCTC	1 cut(s)	584
MboI	GATC	5 cut(s)	15, 254, 717, 777, 793
MboII	GAAGA	7 cut(s)	32, 80, 144, 301, 344, 595, 709
MflI	RGATCY	3 cut(s)	15, 254, 793
MhlI	GDGCHC	1 cut(s)	460
MluCI	AATT	5 cut(s)	71, 196, 295, 415, 451
MmeI	TCCRAC	2 cut(s)	180, 371
MnlI	CCTC	9 cut(s)	29, 48, 112, 176, 274, 706, 759, 805, 931
Mph1103I	ATGCAT	1 cut(s)	892
MroXI	GAANNNNTTC	2 cut(s)	522, 676
MseI	TTAA	2 cut(s)	315, 948
MspA1I	CMGCKG	3 cut(s)	410, 548, 916
MspI	CCGG	1 cut(s)	882
MspR9I	CCNGG	2 cut(s)	234, 461
MvaI	CCWGG	2 cut(s)	234, 461
MwoI	GCNNNNNNNGC	5 cut(s)	113, 185, 439, 542, 887
NdeII	GATC	5 cut(s)	15, 254, 717, 777, 793
NlaIII	CATG	5 cut(s)	139, 360, 545, 640, 692
NlaIV	GGNNCC	2 cut(s)	458, 880
NmuCI	GTSAC	1 cut(s)	909
NsiI	ATGCAT	1 cut(s)	892
NspI	RCATGY	2 cut(s)	360, 545
PaeR7I	CTCGAG	1 cut(s)	8
PciI	ACATGT	1 cut(s)	356
PdmI	GAANNNNTTC	2 cut(s)	522, 676
PfeI	GAWTC	2 cut(s)	518, 574
PkrI	GCNGC	5 cut(s)	412, 547, 550, 967, 995
PscI	ACATGT	1 cut(s)	356
Psp1406I	AACGTT	1 cut(s)	972
Psp6I	CCWGG	2 cut(s)	232, 459
PspFI	CCCAGC	1 cut(s)	491
PspGI	CCWGG	2 cut(s)	232, 459
PspN4I	GGNNCC	2 cut(s)	458, 880
PspOMI	GGGCCC	1 cut(s)	456
PspPI	GGNCC	2 cut(s)	456, 457
PspXI	VCTCGAGB	1 cut(s)	8
PstNI	CAGNNNCTG	1 cut(s)	797
PsuI	RGATCY	3 cut(s)	15, 254, 793
PvuII	CAGCTG	3 cut(s)	410, 548, 916
RsaI	GTAC	4 cut(s)	140, 615, 635, 757
RsaNI	GTAC	4 cut(s)	139, 614, 634, 756
SalI	GTCGAC	1 cut(s)	62
SaqAI	TTAA	2 cut(s)	315, 948
SatI	GCNGC	5 cut(s)	411, 546, 549, 966, 994
Sau3AI	GATC	5 cut(s)	15, 254, 717, 777, 793
Sau96I	GGNCC	2 cut(s)	456, 457
ScrFI	CCNGG	2 cut(s)	234, 461
SduI	GDGCHC	1 cut(s)	460
SfaNI	GCATC	4 cut(s)	136, 210, 758, 877
Sfr274I	CTCGAG	1 cut(s)	8
SgrDI	CGTCGACG	1 cut(s)	62
SlaI	CTCGAG	1 cut(s)	8
SmlI	CTYRAG	1 cut(s)	8
SmoI	CTYRAG	1 cut(s)	8
SpeI	ACTAGT	1 cut(s)	242
Sse9I	AATT	5 cut(s)	71, 196, 295, 415, 451
SsiI	CCGC	1 cut(s)	582
SspI	AATATT	2 cut(s)	326, 479
SspMI	CTAG	1 cut(s)	243
StyD4I	CCNGG	2 cut(s)	232, 459
TaaI	ACNGT	3 cut(s)	508, 775, 944
TaiI	ACGT	1 cut(s)	975
TaqI	TCGA	4 cut(s)	9, 45, 63, 711
TaqII	GACCGA	1 cut(s)	677
TasI	AATT	5 cut(s)	71, 196, 295, 415, 451
TatI	WGTACW	1 cut(s)	613
TfiI	GAWTC	2 cut(s)	518, 574
Tru1I	TTAA	2 cut(s)	315, 948
Tru9I	TTAA	2 cut(s)	315, 948
TscAI	CASTG	1 cut(s)	221
TseFI	GTSAC	1 cut(s)	909
TseI	GCWGC	5 cut(s)	410, 545, 548, 965, 993
Tsp45I	GTSAC	1 cut(s)	909
TspDTI	ATGAA	5 cut(s)	302, 345, 580, 587, 677
TspGWI	ACGGA	1 cut(s)	621
TspRI	CASTG	1 cut(s)	221
XagI	CCTNNNNNAGG	1 cut(s)	679
XapI	RAATTY	2 cut(s)	71, 196
XceI	RCATGY	2 cut(s)	360, 545
XhoI	CTCGAG	1 cut(s)	8
XmiI	GTMKAC	2 cut(s)	63, 301
XmnI	GAANNNNTTC	2 cut(s)	522, 676
XspI	CTAG	1 cut(s)	243
Zsp2I	ATGCAT	1 cut(s)	892

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.