AT5G04895

RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005499
Physical Location & Seq
Forward (+)
1428597 .. 1434769
6173 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04895.1

Sequence Viewer

Length: 3486 bp
ATGCGTTTCACAAAAAGAATAAGCCTCTTCTTGGGGCAGACGACCAGGATTCATAGTCGCTCTCTATTGGGAAATCCGACTTTATGCAGGTCTTATATCGTTGGTCCGGTCTCTACTAGTTCTCCGGCGTTTCTGATTTCGAGTCGTAATGGTGATGGTGTTACGGGATTGTACTACCCGATACGTCGTCGTTTCATCGGGCACACTGCGGAACAGTTCTCGGATGATGAGTACGAGTGTGAATTCGAGGAACATAAGGCTTCATCTTCTGTGGCCAATGTTGATGAGTGGAAGTGGAAGTTAGGAATTTTGTTGGCCAATGATAGTGAACAAGAAATTGTTTCCAGAGACAAAAGGGATCGAAGAGATTATGAGCAGATCTCAAATCTTGCTAAGAGAATGGGACTTTACAGTGAAATTTATGGAAAAGTGGTTGTTGCAAGCAAGGTTCCTCTTCCTAACTACCGACCAGATTTGGATGATAAGCGACCACAACGGGAGGTGGTGCTTCCGTTGAGTTTGCAAAGAAGGGTAGAGGGACTCCTCCAGGAACATCTTGATAGTCAGCAGCTAAGCTCAGGAAAGGCTAATGAATGCGTAGCTGATTCTCAACCTCCTAAACAGACTGAAGAACTTCCCGATGAAAATTCTGATTCGTTTCTCGATGGTTCTGTTATGGAGAAGGTACTTCAGAGGAGGAGTATGCGGATGCGCAATATGCAGAGAACCTGGCAGGAATCCCCTGAGGGACGGACGATGCTGGAATTCAGGAAAACTTTGCCTTCATTCAAGGACAAAGAAAGACTTCTTCAAGCTATTGCTCGGAACCAGGTGATAGTTGTATCTGGGGAGACTGGATGTGGTAAGACAACTCAGCTTCCACAATACATTTTGGAATCAGAGATAGAGTCTGGTCGGGGAGCATTCTGCAACATCATTTGTACTCAACCTAGGAGAATATCTGCCATGGCAGTTTCAGAAAGAGTGTCAGCTGAAAGAGGAGAACCTCTTGGTGAAACGGTTGGGTTTAAAGTTCGTCTAGAAGGAATGAGAGGAAAGAATACCCACCTTCTATTTTGTACAAGTGGTATCCTTCTCAGACGGCTGCTTAGTGATCGCAATTTGAATGGAGTTACACATGTTTTTGTTGATGAGATTCATGAAAGGGGCATGAATGAAGATTTCTTAATTATAGTGTTAAAGGAGCTTCTTCCACGGCGTCCAGATCTGAGATTGGTTCTCATGAGTGCCACTTTGAATGCTGAACTCTTTTCTAATTACTATGGAGGGGCACCTACCATTCACATTCCTGGCTTCACACATCCAGTGAAAGCACATTTTTTGGAGGATGTACTGGAAATAACGGGATATAAGCTGACCTCTTTTAATCAAGTTGATGATTATGGCCAAGAAAAGACGTGGAAAACGCAAAAACAGTTAATGCCTCGCAAAAGAAAAAACCAGATCACTACTCTTGTTGAGGAAGCTCTCAGCAAGTCAAATTTTGAAAGCTATAACTCTAGGACACGTGATTCTCTTTCGTCTTGGATGCCTGACTGCATAGGATTTAATCTCATTGAGGCTGTTCTCTGTCACATATGCCGAAAAGAACGTCCAGGTGCTGTATTAGTATTTTTGACAGGATGGGATGATATCAGATCCCTGAGCGACCAAATTAAAGCCCATCCTCTCTTGGGAGATCCGAACAGGGTTTTGCTATTAATGTGCCACGGTTCAATGGCAACTGCTGAGCAGAGACTCATTTTTGAGAGAGCACCTCCTAATATTCGCAAAATTGTTCTTGCTACAAACATGGCAGAAGCTAGTATTACAATAAACGATGTAGTGTTTGTGGTTGACTGTGGAAAAGCAAAAGAGACCACCTATGATGCTTTGAACAATACACCTTGTCTGCTACCTTCTTGGATATCTCAAGCTTCTGCGAGACAGAGAAGGGGAAGAGCTGGGCGTCTGTTTCCGGGAGAATGCTATCACCTGTACCCCAAATGCGTCTATGATGCTTTTGCAGAGTATCAACTTCCTGAACTTCTGAGAACTCCTTTAAACTCTCTCTGCCTGCAAATTAAAAGTCTTCAGGTTGAAAGCATTGCAGAATTCTTGTCAGCTGCACTTCAGGCTCCAGAGTCTTTGGCTGTTCAAAATGCTATTGGATTCCTGAAAATGATCGGAGCACTGGATGAGAAGGAAAATCTAACGGATCTTGGAAAACTCTTATCGATTCTTCCTGTTGATCCCAAGTTGGGGAAAATGCTGATAATGGGTGCCATCTTCCGCTGCTTTGATCCGATCCTGACAATTGTATCAGGGCTAAGTGTCAGAGATCCTTTCCTTCTACCTCAAGACAAAAAAGACTTAGCTTTATCAGCCAAGTTGAGATTCTCAGCCAAAGACTACAGTGACCATATGGCACTCGTTCGTGCCTTTGAAGGGTGGAAAGATGCTGAAAGAGAAGGATCGGCTTATGAGTTTTGTTGGAGGAATTTCCTGTCAGCTCAGACGCTTCAGGCGATACATTCTCTGAGGAAGCAGTTCAACTATATTTTGAAAGAAGCTGGCTTAGTTCATGATGATTTAGCTCTCAACAACAAGCTAAGTCACAACCAGTCTCTGGTTCGTGCTGTCATTTGCTCTGGCCTATTCCCTGGAATAGCATCTGTTGTGCACAGAGAGACTTCCATGTCATTCAAGACAATGGATGATGGCCAAGTATCACTATACGCGAATTCTGTTAATTCGCGGTTCCCAACAATTCCATACCCTTGGCTAGTCTTTGGAGAGAAAGTAAAGGTCAATGCGGTACTCATACGGGACTCCACTGGTGTCCCTGATTCCAGTTTGATCCTTTTTGGTGGTTCATTGAGCACGGGAGTACAGGTGGGACATCTAAAAATGCTTGACGGGTACATTGATTTCTTCATGGATCCAAATTTGGCTGAGTCCTATGTAAAGCTGAAGGAAGAACTCGATAAGCTTCTTCAAAAAAAGCTTGAAGATCCAAGCATGGACATCCACAAAGAAGGCAAGTACCTCATGCTTGCAGTTCAAGAACTTGTGGCTGGAGACCAATGCGAAGGCAGATTTGTCTTTGGCCGTGACACAAAGAGGCCAAGCCAACCGCAAATAGGAGAGAACAAACACTCCAAAGACGGGACAAACCCGAAGAGTCTCCTTCAGACACTTTTGATGAGAGCTGGACACTCACCTCCAAAGTACAAGACAAAACATCTCAAGACAAACGAATTCAGAGCACTTGTGGAGTTTAAAGGAATGCAGTTCGTTGGGAAGCCTCAGAGGAATAAAACGCTCGCTGAGAAAGATGCTGCTGTTGAGGCCTTAGCTTGGTTGACTCATACCTCTGATAATAGTACTGGCCAACACAATGAGGATGCAGATTCTCCACCTGATGTTACTGATAACATGCTTAAACTCCTTGGAGGACGGCGCAGGCGATCCAAGGGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1161

Amino Acids

130.83

Weight (kDa)

8.63

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 267 - 422 1.4e-08 DEAD/DEAH box helicase
Helicase_C PF00271 529 - 658 1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 722 - 750 1.8e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 751 - 811 1.9e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 878 - 959 1.4e-16 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 968 - 1026 1.9e-24 C-terminal domain in RNA helicases
dsrm PF00035 1060 - 1122 1.3e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2698
Acc16I TGCGCA 1 cut(s) 713
Acc36I ACCTGC 1 cut(s) 78
AccB1I GGYRCC 2 cut(s) 1291, 2282
AccB7I CCANNNNNTGG 1 cut(s) 2629
AccII CGCG 2 cut(s) 2741, 2758
AciI CCGC 6 cut(s) 209, 706, 2293, 2758, 2816, 3137
AcoI YGGCCR 6 cut(s) 273, 315, 1405, 2722, 3109, 3391
AcuI CTGAAG 7 cut(s) 648, 674, 2078, 2117, 2507, 2993, 3176
AcvI CACGTG 1 cut(s) 1529
AcyI GRCGYC 2 cut(s) 1219, 1969
AfiI CCNNNNNNNGG 9 cut(s) 31, 1694, 2261, 2262, 2448, 2629, 3143, 3168, 3360
AflIII ACRYGT 2 cut(s) 1138, 1526
AhlI ACTAGT 1 cut(s) 116
AjiI CACGTC 1 cut(s) 1419
AjnI CCWGG 7 cut(s) 44, 546, 728, 828, 1309, 1615, 2662
AjuI GAANNNNNNNTTGG 2 cut(s) 2381, 2413
AloI GAACNNNNNNTCC 2 cut(s) 3143, 3175
Alw21I GWGCWC 5 cut(s) 1777, 2194, 2685, 2885, 3271
Alw44I GTGCAC 1 cut(s) 2681
AlwNI CAGNNNCTG 2 cut(s) 1622, 2629
AoxI GGCC 9 cut(s) 273, 315, 1405, 2653, 2722, 3109, 3125, 3351, 3391
ApaLI GTGCAC 1 cut(s) 2681
ApeKI GCWGC 5 cut(s) 568, 1105, 2126, 2295, 3341
ArsI GACNNNNNNTTYG 4 cut(s) 1597, 1629, 2074, 2106
AseI ATTAAT 1 cut(s) 1721
Asp700I GAANNNNTTC 3 cut(s) 633, 804, 2549
AspA2I CCTAGG 1 cut(s) 950
AspLEI GCGC 2 cut(s) 714, 3465
AspS9I GGNCC 1 cut(s) 104
AsuC2I CCSGG 1 cut(s) 1980
AsuHPI GGTGA 5 cut(s) 164, 844, 1025, 1985, 3213
AvaII GGWCC 1 cut(s) 104
AvrII CCTAGG 1 cut(s) 950
AxyI CCTNAGG 1 cut(s) 744
BaeGI GKGCMC 3 cut(s) 204, 1294, 2685
BaeI ACNNNNGTAYC 2 cut(s) 2304, 2337
BalI TGGCCA 5 cut(s) 275, 317, 1407, 2724, 3393
BamHI GGATCC 1 cut(s) 2941
BanI GGYRCC 2 cut(s) 1291, 2282
BbrPI CACGTG 1 cut(s) 1529
BbsI GAAGAC 1 cut(s) 2084
Bbv12I GWGCWC 5 cut(s) 1777, 2194, 2685, 2885, 3271
BbvI GCAGC 5 cut(s) 580, 1092, 2113, 2282, 3328
BccI CCATC 6 cut(s) 149, 659, 1638, 1692, 2294, 2714
BceAI ACGGC 4 cut(s) 1118, 1232, 3096, 3476
BciT130I CCWGG 7 cut(s) 46, 548, 730, 830, 1311, 1617, 2664
BciVI GTATCC 1 cut(s) 1100
BcnI CCSGG 1 cut(s) 1980
BcuI ACTAGT 1 cut(s) 116
BfaI CTAG 6 cut(s) 117, 951, 1040, 1521, 1824, 2786
BfmI CTRYAG 1 cut(s) 2413
BfuAI ACCTGC 1 cut(s) 78
BfuI GTATCC 1 cut(s) 1100
BglII AGATCT 2 cut(s) 378, 1225
BisI GCNGC 5 cut(s) 569, 1106, 2127, 2296, 3342
BlnI CCTAGG 1 cut(s) 950
BlpI GCTNAGC 2 cut(s) 572, 1749
BlsI GCNGC 5 cut(s) 570, 1107, 2128, 2297, 3343
BmcAI AGTACT 1 cut(s) 3388
Bme1390I CCNGG 8 cut(s) 46, 548, 730, 830, 1311, 1617, 1980, 2664
Bme18I GGWCC 1 cut(s) 104
BmgBI CACGTC 1 cut(s) 1419
BmgT120I GGNCC 1 cut(s) 104
BmiI GGNNCC 7 cut(s) 450, 827, 1293, 2139, 2284, 2762, 2943
BmrFI CCNGG 8 cut(s) 46, 548, 730, 830, 1311, 1617, 1980, 2664
BmsI GCATC 9 cut(s) 699, 747, 1539, 1879, 2008, 2449, 2681, 3328, 3397
BpiI GAAGAC 1 cut(s) 2084
BplI GAGNNNNNCTC 4 cut(s) 365, 397, 1762, 1794
BpmI CTGGAG 3 cut(s) 530, 2124, 3099
Bpu10I CCTNAGC 3 cut(s) 577, 1664, 3355
Bpu1102I GCTNAGC 2 cut(s) 572, 1749
BpuEI CTTGAG 3 cut(s) 1917, 2343, 3233
BpuMI CCSGG 1 cut(s) 1980
Bsa29I ATCGAT 1 cut(s) 2237
BsaAI YACGTR 1 cut(s) 1529
BsaHI GRCGYC 2 cut(s) 1219, 1969
BsaI GGTCTC 3 cut(s) 115, 1871, 3075
BsaJI CCNNGG 8 cut(s) 950, 966, 1214, 1729, 2662, 2780, 3451, 3474
BsaWI WCCGGW 1 cut(s) 106
BsaXI ACNNNNNCTCC 8 cut(s) 106, 136, 2787, 2817, 2880, 2910, 3143, 3173
Bsc4I CCNNNNNNNGG 9 cut(s) 31, 1694, 2261, 2262, 2448, 2629, 3143, 3168, 3360
Bse1I ACTGG 8 cut(s) 859, 1325, 1359, 2199, 2623, 2842, 2853, 3394
Bse21I CCTNAGG 1 cut(s) 744
Bse3DI GCAATG 1 cut(s) 2106
BseBI CCWGG 7 cut(s) 46, 548, 730, 830, 1311, 1617, 2664
BseCI ATCGAT 1 cut(s) 2237
BseDI CCNNGG 8 cut(s) 950, 966, 1214, 1729, 2662, 2780, 3451, 3474
BseLI CCNNNNNNNGG 9 cut(s) 31, 1694, 2261, 2262, 2448, 2629, 3143, 3168, 3360
BseMI GCAATG 1 cut(s) 2106
BseNI ACTGG 8 cut(s) 859, 1325, 1359, 2199, 2623, 2842, 2853, 3394
BseRI GAGGAG 4 cut(s) 533, 709, 712, 1014
BseSI GKGCMC 3 cut(s) 204, 1294, 2685
BseXI GCAGC 5 cut(s) 580, 1092, 2113, 2282, 3328
BseYI CCCAGC 1 cut(s) 1964
BsgI GTGCAG 1 cut(s) 2112
Bsh1236I CGCG 2 cut(s) 2741, 2758
BshFI GGCC 9 cut(s) 275, 317, 1407, 2655, 2724, 3111, 3127, 3353, 3393
BshNI GGYRCC 2 cut(s) 1291, 2282
BshVI ATCGAT 1 cut(s) 2237
BsiHKAI GWGCWC 5 cut(s) 1777, 2194, 2685, 2885, 3271
BsiSI CCGG 3 cut(s) 107, 125, 1979
BslFI GGGAC 7 cut(s) 417, 552, 762, 2828, 2843, 2913, 3184
BslI CCNNNNNNNGG 9 cut(s) 31, 1694, 2261, 2262, 2448, 2629, 3143, 3168, 3360
BsmFI GGGAC 7 cut(s) 417, 552, 762, 2828, 2843, 2913, 3184
BsmI GAATGC 5 cut(s) 599, 923, 1264, 1991, 3294
BsnI GGCC 9 cut(s) 275, 317, 1407, 2655, 2724, 3111, 3127, 3353, 3393
Bso31I GGTCTC 3 cut(s) 115, 1871, 3075
Bsp1286I GDGCHC 7 cut(s) 204, 1294, 1777, 2194, 2685, 2885, 3271
Bsp1407I TGTACA 1 cut(s) 1079
Bsp1720I GCTNAGC 2 cut(s) 572, 1749
Bsp19I CCATGG 1 cut(s) 966
BspACI CCGC 6 cut(s) 209, 706, 2293, 2758, 2816, 3137
BspANI GGCC 9 cut(s) 275, 317, 1407, 2655, 2724, 3111, 3127, 3353, 3393
BspDI ATCGAT 1 cut(s) 2237
BspFNI CGCG 2 cut(s) 2741, 2758
BspHI TCATGA 3 cut(s) 1159, 1242, 2584
BspLI GGNNCC 7 cut(s) 450, 827, 1293, 2139, 2284, 2762, 2943
BspMI ACCTGC 1 cut(s) 78
BspQI GCTCTTC 1 cut(s) 1954
BspT107I GGYRCC 2 cut(s) 1291, 2282
BspTNI GGTCTC 3 cut(s) 115, 1871, 3075
BsrDI GCAATG 1 cut(s) 2106
BsrGI TGTACA 1 cut(s) 1079
BsrI ACTGG 8 cut(s) 859, 1325, 1359, 2199, 2623, 2842, 2853, 3394
BssECI CCNNGG 8 cut(s) 950, 966, 1214, 1729, 2662, 2780, 3451, 3474
BssNI GRCGYC 2 cut(s) 1219, 1969
BssT1I CCWWGG 5 cut(s) 950, 966, 2780, 3451, 3474
Bst2UI CCWGG 7 cut(s) 46, 548, 730, 830, 1311, 1617, 2664
Bst4CI ACNGT 7 cut(s) 216, 413, 1021, 1437, 1733, 1862, 2417
Bst6I CTCTTC 5 cut(s) 32, 358, 459, 1954, 3176
BstACI GRCGYC 2 cut(s) 1219, 1969
BstAUI TGTACA 1 cut(s) 1079
BstBAI YACGTR 1 cut(s) 1529
BstC8I GCNNGC 6 cut(s) 442, 2078, 2575, 3057, 3327, 3467
BstDSI CCRYGG 3 cut(s) 966, 1214, 1729
BstFNI CGCG 2 cut(s) 2741, 2758
BstHHI GCGC 2 cut(s) 714, 3465
BstMWI GCNNNNNNNGC 4 cut(s) 718, 2135, 2384, 3350
BstNI CCWGG 7 cut(s) 46, 548, 730, 830, 1311, 1617, 2664
BstNSI RCATGY 2 cut(s) 1142, 3442
BstSCI CCNGG 8 cut(s) 44, 546, 728, 828, 1309, 1615, 1978, 2662
BstSFI CTRYAG 1 cut(s) 2413
BstSLI GKGCMC 3 cut(s) 204, 1294, 2685
BstUI CGCG 2 cut(s) 2741, 2758
BstV1I GCAGC 5 cut(s) 580, 1092, 2113, 2282, 3328
BstV2I GAAGAC 1 cut(s) 2084
BstX2I RGATCY 8 cut(s) 378, 1225, 1658, 1699, 2218, 2341, 2941, 3013
BstXI CCANNNNNNTGG 1 cut(s) 2781
BstYI RGATCY 8 cut(s) 378, 1225, 1658, 1699, 2218, 2341, 2941, 3013
Bsu15I ATCGAT 1 cut(s) 2237
Bsu36I CCTNAGG 1 cut(s) 744
BsuI GTATCC 1 cut(s) 1100
BsuRI GGCC 9 cut(s) 275, 317, 1407, 2655, 2724, 3111, 3127, 3353, 3393
BsuTUI ATCGAT 1 cut(s) 2237
BtgI CCRYGG 3 cut(s) 966, 1214, 1729
BtrI CACGTC 1 cut(s) 1419
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 6 cut(s) 204, 418, 1332, 2192, 2422, 2835
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 6 cut(s) 442, 2078, 2575, 3057, 3327, 3467
CaiI CAGNNNCTG 2 cut(s) 1622, 2629
CciI TCATGA 3 cut(s) 1159, 1242, 2584
CfoI GCGC 2 cut(s) 714, 3465
Cfr13I GGNCC 1 cut(s) 104
ClaI ATCGAT 1 cut(s) 2237
CseI GACGC 4 cut(s) 1208, 1958, 1999, 2527
CsiI ACCWGGT 1 cut(s) 828
DraI TTTAAA 3 cut(s) 1030, 2064, 3283
DrdI GACNNNNNNGTC 1 cut(s) 2698
DseDI GACNNNNNNGTC 1 cut(s) 2698
EaeI YGGCCR 6 cut(s) 273, 315, 1405, 2722, 3109, 3391
Eam1104I CTCTTC 5 cut(s) 32, 358, 459, 1954, 3176
EarI CTCTTC 5 cut(s) 32, 358, 459, 1954, 3176
Eco130I CCWWGG 5 cut(s) 950, 966, 2780, 3451, 3474
Eco147I AGGCCT 1 cut(s) 3353
Eco31I GGTCTC 3 cut(s) 115, 1871, 3075
Eco32I GATATC 2 cut(s) 1654, 1929
Eco47I GGWCC 1 cut(s) 104
Eco57I CTGAAG 7 cut(s) 648, 674, 2078, 2117, 2507, 2993, 3176
Eco72I CACGTG 1 cut(s) 1529
Eco81I CCTNAGG 1 cut(s) 744
EcoRI GAATTC 5 cut(s) 242, 764, 2114, 2743, 3260
EcoRII CCWGG 7 cut(s) 44, 546, 728, 828, 1309, 1615, 2662
EcoRV GATATC 2 cut(s) 1654, 1929
EcoT14I CCWWGG 5 cut(s) 950, 966, 2780, 3451, 3474
ErhI CCWWGG 5 cut(s) 950, 966, 2780, 3451, 3474
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FaqI GGGAC 7 cut(s) 417, 552, 762, 2828, 2843, 2913, 3184
FauNDI CATATG 2 cut(s) 1598, 2424
Fnu4HI GCNGC 5 cut(s) 569, 1106, 2127, 2296, 3342
Fsp4HI GCNGC 5 cut(s) 569, 1106, 2127, 2296, 3342
FspBI CTAG 6 cut(s) 117, 951, 1040, 1521, 1824, 2786
FspI TGCGCA 1 cut(s) 713
GlaI GCGC 2 cut(s) 713, 3464
GluI GCNGC 5 cut(s) 569, 1106, 2127, 2296, 3342
GsaI CCCAGC 1 cut(s) 1968
GsuI CTGGAG 3 cut(s) 530, 2124, 3099
HaeIII GGCC 9 cut(s) 275, 317, 1407, 2655, 2724, 3111, 3127, 3353, 3393
HapII CCGG 3 cut(s) 107, 125, 1979
HgaI GACGC 4 cut(s) 1208, 1958, 1999, 2527
HhaI GCGC 2 cut(s) 714, 3465
Hin1I GRCGYC 2 cut(s) 1219, 1969
Hin6I GCGC 2 cut(s) 712, 3463
HinP1I GCGC 2 cut(s) 712, 3463
HincII GTYRAC 2 cut(s) 1858, 3366
HindII GTYRAC 2 cut(s) 1858, 3366
HindIII AAGCTT 3 cut(s) 1935, 2990, 3005
HpaII CCGG 3 cut(s) 107, 125, 1979
HphI GGTGA 5 cut(s) 164, 844, 1025, 1985, 3213
Hpy166II GTNNAC 4 cut(s) 329, 1858, 2683, 3366
Hpy8I GTNNAC 4 cut(s) 329, 1858, 2683, 3366
Hpy99I CGWCG 2 cut(s) 189, 192
HpyCH4III ACNGT 7 cut(s) 216, 413, 1021, 1437, 1733, 1862, 2417
HpyCH4IV ACGT 4 cut(s) 184, 1418, 1528, 1612
HpyF10VI GCNNNNNNNGC 4 cut(s) 718, 2135, 2384, 3350
HpySE526I ACGT 4 cut(s) 184, 1418, 1528, 1612
Hsp92I GRCGYC 2 cut(s) 1219, 1969
HspAI GCGC 2 cut(s) 712, 3463
LguI GCTCTTC 1 cut(s) 1954
LmnI GCTCC 4 cut(s) 920, 1204, 2143, 2189
Lsp1109I GCAGC 5 cut(s) 580, 1092, 2113, 2282, 3328
LweI GCATC 9 cut(s) 699, 747, 1539, 1879, 2008, 2449, 2681, 3328, 3397
MabI ACCWGGT 1 cut(s) 828
MaeI CTAG 6 cut(s) 117, 951, 1040, 1521, 1824, 2786
MaeII ACGT 4 cut(s) 184, 1418, 1528, 1612
MaeIII GTNAC 7 cut(s) 160, 1132, 1592, 2417, 2615, 3113, 3427
MfeI CAATTG 1 cut(s) 2316
MflI RGATCY 8 cut(s) 378, 1225, 1658, 1699, 2218, 2341, 2941, 3013
MhlI GDGCHC 7 cut(s) 204, 1294, 1777, 2194, 2685, 2885, 3271
MlsI TGGCCA 5 cut(s) 275, 317, 1407, 2724, 3393
MluNI TGGCCA 5 cut(s) 275, 317, 1407, 2724, 3393
MlyI GAGTC 9 cut(s) 151, 534, 917, 1752, 2153, 2825, 2966, 3193, 3361
MmeI TCCRAC 2 cut(s) 101, 2474
Mox20I TGGCCA 5 cut(s) 275, 317, 1407, 2724, 3393
MroXI GAANNNNTTC 3 cut(s) 633, 804, 2549
MscI TGGCCA 5 cut(s) 275, 317, 1407, 2724, 3393
Msp20I TGGCCA 5 cut(s) 275, 317, 1407, 2724, 3393
MspA1I CMGCKG 3 cut(s) 992, 2126, 2295
MspI CCGG 3 cut(s) 107, 125, 1979
MspR9I CCNGG 8 cut(s) 46, 548, 730, 830, 1311, 1617, 1980, 2664
MunI CAATTG 1 cut(s) 2316
Mva1269I GAATGC 5 cut(s) 599, 923, 1264, 1991, 3294
MvaI CCWGG 7 cut(s) 46, 548, 730, 830, 1311, 1617, 2664
MvnI CGCG 2 cut(s) 2741, 2758
MwoI GCNNNNNNNGC 4 cut(s) 718, 2135, 2384, 3350
NciI CCSGG 1 cut(s) 1980
NcoI CCATGG 1 cut(s) 966
NdeI CATATG 2 cut(s) 1598, 2424
NlaIV GGNNCC 7 cut(s) 450, 827, 1293, 2139, 2284, 2762, 2943
NmuCI GTSAC 4 cut(s) 1592, 2417, 2615, 3113
NsbI TGCGCA 1 cut(s) 713
NspI RCATGY 2 cut(s) 1142, 3442
PagI TCATGA 3 cut(s) 1159, 1242, 2584
PceI AGGCCT 1 cut(s) 3353
PciI ACATGT 1 cut(s) 1138
PciSI GCTCTTC 1 cut(s) 1954
PcsI WCGNNNNNNNCGW 2 cut(s) 2525, 3466
PctI GAATGC 5 cut(s) 599, 923, 1264, 1991, 3294
PdmI GAANNNNTTC 3 cut(s) 633, 804, 2549
PflMI CCANNNNNTGG 1 cut(s) 2629
PfoI TCCNGGA 2 cut(s) 546, 1978
PkrI GCNGC 5 cut(s) 570, 1107, 2128, 2297, 3343
PleI GAGTC 9 cut(s) 150, 534, 916, 1752, 2152, 2825, 2965, 3192, 3361
PmaCI CACGTG 1 cut(s) 1529
PmlI CACGTG 1 cut(s) 1529
PpsI GAGTC 9 cut(s) 150, 534, 916, 1752, 2152, 2825, 2965, 3192, 3361
Ppu21I YACGTR 1 cut(s) 1529
PscI ACATGT 1 cut(s) 1138
PshBI ATTAAT 1 cut(s) 1721
Psp6I CCWGG 7 cut(s) 44, 546, 728, 828, 1309, 1615, 2662
PspCI CACGTG 1 cut(s) 1529
PspFI CCCAGC 1 cut(s) 1964
PspGI CCWGG 7 cut(s) 44, 546, 728, 828, 1309, 1615, 2662
PspN4I GGNNCC 7 cut(s) 450, 827, 1293, 2139, 2284, 2762, 2943
PspPI GGNCC 1 cut(s) 104
PstNI CAGNNNCTG 2 cut(s) 1622, 2629
PsuI RGATCY 8 cut(s) 378, 1225, 1658, 1699, 2218, 2341, 2941, 3013
PvuII CAGCTG 2 cut(s) 992, 2126
SapI GCTCTTC 1 cut(s) 1954
SatI GCNGC 5 cut(s) 569, 1106, 2127, 2296, 3342
Sau96I GGNCC 1 cut(s) 104
ScaI AGTACT 1 cut(s) 3388
SchI GAGTC 9 cut(s) 151, 534, 917, 1752, 2153, 2825, 2966, 3193, 3361
ScrFI CCNGG 8 cut(s) 46, 548, 730, 830, 1311, 1617, 1980, 2664
SduI GDGCHC 7 cut(s) 204, 1294, 1777, 2194, 2685, 2885, 3271
SexAI ACCWGGT 1 cut(s) 828
SfaNI GCATC 9 cut(s) 699, 747, 1539, 1879, 2008, 2449, 2681, 3328, 3397
SfcI CTRYAG 1 cut(s) 2413
SinI GGWCC 1 cut(s) 104
SmlI CTYRAG 3 cut(s) 1932, 2358, 3248
SmoI CTYRAG 3 cut(s) 1932, 2358, 3248
SpeI ACTAGT 1 cut(s) 116
SseBI AGGCCT 1 cut(s) 3353
SsiI CCGC 6 cut(s) 209, 706, 2293, 2758, 2816, 3137
SspI AATATT 1 cut(s) 1786
SspMI CTAG 6 cut(s) 117, 951, 1040, 1521, 1824, 2786
StuI AGGCCT 1 cut(s) 3353
StyD4I CCNGG 8 cut(s) 44, 546, 728, 828, 1309, 1615, 1978, 2662
StyI CCWWGG 5 cut(s) 950, 966, 2780, 3451, 3474
TaaI ACNGT 7 cut(s) 216, 413, 1021, 1437, 1733, 1862, 2417
TaiI ACGT 4 cut(s) 187, 1421, 1531, 1615
TaqI TCGA 6 cut(s) 140, 246, 361, 663, 2237, 2985
TatI WGTACW 7 cut(s) 171, 941, 1079, 1351, 2890, 3231, 3386
TscAI CASTG 6 cut(s) 211, 418, 1332, 2199, 2422, 2842
TseFI GTSAC 4 cut(s) 1592, 2417, 2615, 3113
TseI GCWGC 5 cut(s) 568, 1105, 2126, 2295, 3341
Tsp45I GTSAC 4 cut(s) 1592, 2417, 2615, 3113
TspGWI ACGGA 3 cut(s) 501, 766, 2231
TspRI CASTG 6 cut(s) 211, 418, 1332, 2199, 2422, 2842
Van91I CCANNNNNTGG 1 cut(s) 2629
VneI GTGCAC 1 cut(s) 2681
VpaK11BI GGWCC 1 cut(s) 104
VspI ATTAAT 1 cut(s) 1721
XbaI TCTAGA 1 cut(s) 1039
XceI RCATGY 2 cut(s) 1142, 3442
XmaJI CCTAGG 1 cut(s) 950
XmnI GAANNNNTTC 3 cut(s) 633, 804, 2549
XspI CTAG 6 cut(s) 117, 951, 1040, 1521, 1824, 2786
ZrmI AGTACT 1 cut(s) 3388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.