Rh4BG132800

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005499
Physical Location & Seq
Forward (+)
22357695 .. 22374491
16797 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG132800.1

Sequence Viewer

Length: 3441 bp
ATGAGAGCGGCGGCGTCTCTCCTTGTCAAATCAGTAGCCTCCTCCAACAATACTTGTTTTCGTTCTCACCACCTCAAATTCTGTCTTGTTGTACTCCAACCACAATCACAACCACACTCTTCCTCTTCTCCCATTTCGATTTCGAAGCGCGGCTTTTCCGGCTACGCTGTGGAGCAGTTCTCCGACGACGAGTATGAGTGCGATTTCGAAAGCCAAAAGGCTTCTTCTTCCGTGGCCAACATTGACGAGTGGAAATGGAAACTGAGCCTGCTGCTGCGAAGTGACAAGGACCAAGAGGTTGTTTCCAGAGATAAAAGGGATAGGAGAGACTATGAGCAAATATCTAACCTCGCCAAACGGATGGGACTTTACACTGAAATATATGGCAAAGTGGTGGTTGCCAGCAAGGTTCCTCTTCCAAACTACCGCCCCGATTTGGATGATAAGCGCCCACAAAGAGAGGTGGTTATCCCTCTTGGCTTGCAAAGGAGGGTAGAGGGTTTATTGCAAGAACACCTTGATCGTGTCCTGCTAAGTTTCCGCAACGTATCAGCCAATTCAGGTGACAGTGACCACTTCGATCAGGTTGAAAATGAAAAGCCTGATGCGAATGCAGATTCTTTTCTTGATGGGTCTGTAATGGAGAAGGTTCTTCAGCGTAGGAGTTTGCGCATGCGTAATATGCAACGAGCTTGGCAGGAATCACCTGAAGGTAAAAAGATGCTAGAGTTCCGGAAATCACTACCATCTTTCAAAGAGAATCAGAGGTTGCTGCAAGCAATAGCACAAAATCAGGTAGTAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAGCTTCCTCAATATATACTAGAGTCAGAGATAGAATCTGGCCGTGGAGCATTCTGTAGCATTATCTGTACACAACCTCGAAGAATATCTGCTATGGCTGTTGCTGAAAGAGTGTCTGCAGAGAGGGGAGAGCCTCTTGGTGAAACAGTGGGCTATAAAGTTCGATTAGAGGGAATGAAAGGAAAAAATACGCATCTCCTTTTCTGTACCAGTGGTATTCTACTTCGCCGTCTGTTGAGTGATCGGAACTTGAGTGGTGTAACACATGTTTTTGTTGATGAAATTCATGAACGAGGCATGAATGAAGACTTCTTGTTGATTGTATTGAAAGATCTGCTTCCACGCCGTCGGGATCTAAGATTGGTTTTGATGAGTGCCACTCTGAATGCGGAGCTATTTTCAAATTTTCTTGGAGGAGCACCAACAATTCACATTCCGGGTTTCACATATCCAGTGAGAGCACACTTTTTGGAAGATGTACTGGAAATGACTGGGTATAAGCTGACTTCTTTCAACCAAATTGATGACTATGGCCAAGACAAACTGTGGAAAACGCAGAAGCAGTTAGCGCCTCGGAAAAGGAAAAACCAGATCACTGCTCTAGTTGAGGATGCTCTTAGTAAATCAAGTTTTGAGAATTATAGCCCCAGAGCACGTGATTCATTATCATGTTGGACGGCTGATTGCATAGGATTTAATCTTATTGAAGCAGTTTTATGTCATATATGTCGGAAGGAACGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGAGGATATCAGCTGTTTGAGGGATCAACTTAAAGCCCATCCTCTTTTAGGTGATCCTAACAGGGTTTTGCTTATAACATGCCATGGGTCAATGACCACGTCTGAACAGAACCTCATTTTCGTGAGACCGCCTCCTAATGTACGTAAAATTGTTCTTGCTACTAATATGGCAGAAGCAAGCATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCACATATGATGCTTTGAATAATACACCTTGTCTACTACCTTCTTGGATATCCCAAGCATCTGCTCGGCAAAGAAGGGGAAGGGCTGGCCGGGTGCAGCCAGGCGAATGTTACCACCTTTACCCCAAATGTGTGTATCATGCGTTTGCAGAATACCAACTTCCTGAACTTCTAAGGACTCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTTTGCAGGTTGGTAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCACCTGAACCCCTTGCTGTTCAAAATGCTGTTGGCTTTCTTAAGTTGATTGGGGCATTGGATGAGAATGAAAATTTGACAAATCTCGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTAAGTGTCCGGGATCCATTCCTTTTGCCTCAAGACAAAAAAGATTTAGCGGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTATAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCAGAAAGAGAAGGATCAGCGTATGAATACTGCTGGAGAAATTTTCTCTCTGCCCAGACACTTCAAGCTATTCATTCTCTTCGGAAGCAGTTTAGCTACATCTTAAGAGACGCTGGCTTGGTGGATGCAGATGCAAGCATTAACAACAAGCTAAGTCACAATCAGTCATTGGTCCGCGCAATCATATGTTCCGGACTATTCCCTGGCATAGCATCTGTAGTGCACCGAGAGACATCCATGTCTTTCAAAACTATGGATGATGGCCAGGTTTTACTATATGCTAATTCTGTAAATGCACGTTACCAAACAATTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAGTAAAGGTCAACACAGTTTTCATACGCGATTCTACTGGTGTCTCTGATTCCATTTTGATCCTTTTTGGTGGTGCCCTAAACCATGGTATCCAGGCTGGGCATTTGAAAATGTTGGACGGCTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGCTTAAAGAAGAGCTTAATGAGCTTATTCAGAAGAAGCTTCAAGATCCAAGCCTCGATATCCACAAGGAAGGAAAGTATCTCATACTTGCTGTTCAAGAGCTGGTATCTGGAGATCAAAGTGAAGGAAGATTTGTGTTTGGCCGTGAAAACAGGAAGCCAAAGGAGTCTGGTGATAACAGCAGATTCACAAGGGATGGAACCAATCCTAAGAGTTTGCTACAAACTCTATTGATGAGAGCAGGACATTCACCTCCAAAATACAAAACCAAGCACCTCAAGACAAATGAGTTCAGAGCACTTGTGGAGTTCAAGGGCATGCAATTTGTTGGGAAGCCCAAGAAAAATAAGCAGCTTGCCGAAAGGGATGCTGCCATAGAAGCACTGGCCTGGTTGACTCACACCTCAGAGAACAGTCGTGATGAAGCTGATAACTCCCCACCTGATGTCACTAACAACATGTTGAAACTTCTGGGGAAACGTAGAAGATCAAAGCGCCACTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1146

Amino Acids

128.99

Weight (kDa)

8.85

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 256 - 410 2.3e-09 DEAD/DEAH box helicase
Helicase_C PF00271 517 - 646 1.5e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 710 - 738 2.8e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 739 - 799 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 866 - 947 5.4e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 956 - 1014 2.6e-23 C-terminal domain in RNA helicases
dsrm PF00035 1047 - 1109 7.7e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1685
AasI GACNNNNNNGTC 1 cut(s) 2662
Acc16I TGCGCA 1 cut(s) 671
Acc36I ACCTGC 1 cut(s) 2050
AccB1I GGYRCC 1 cut(s) 2837
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 1876
AccII CGCG 3 cut(s) 150, 2601, 2793
AccIII TCCGGA 2 cut(s) 732, 2615
AciI CCGC 9 cut(s) 8, 11, 150, 427, 541, 1226, 1739, 2342, 2599
AcoI YGGCCR 7 cut(s) 234, 877, 1369, 1577, 1930, 2686, 3073
AcsI RAATTY 7 cut(s) 77, 1119, 1240, 2029, 2078, 2173, 2464
AcuI CTGAAG 2 cut(s) 638, 729
AcvI CACGTG 1 cut(s) 1493
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 5 cut(s) 93, 907, 1045, 1317, 1752
AfiI CCNNNNNNNGG 6 cut(s) 436, 1658, 2225, 2226, 2745, 2749
AflII CTTAAG 2 cut(s) 2141, 2527
AflIII ACRYGT 2 cut(s) 1102, 3390
AjiI CACGTC 1 cut(s) 1710
AjnI CCWGG 6 cut(s) 1579, 1942, 2626, 2688, 2856, 3320
Alw21I GWGCWC 5 cut(s) 1258, 1300, 1492, 2649, 3232
Alw26I GTCTC 8 cut(s) 21, 321, 809, 1729, 1835, 2526, 2648, 2812
Alw44I GTGCAC 1 cut(s) 2645
AlwNI CAGNNNCTG 1 cut(s) 1586
Aor13HI TCCGGA 2 cut(s) 732, 2615
AoxI GGCC 8 cut(s) 234, 877, 1369, 1577, 1930, 2686, 3073, 3318
ApaLI GTGCAC 1 cut(s) 2645
ApeKI GCWGC 7 cut(s) 271, 274, 772, 1939, 2090, 3283, 3302
ApoI RAATTY 7 cut(s) 77, 1119, 1240, 2029, 2078, 2173, 2464
AspLEI GCGC 6 cut(s) 150, 450, 672, 1408, 2603, 3429
AspS9I GGNCC 2 cut(s) 289, 2596
AsuC2I CCSGG 3 cut(s) 1275, 1934, 2303
AsuHPI GGTGA 8 cut(s) 59, 575, 696, 989, 1673, 2771, 3116, 3174
AsuII TTCGAA 2 cut(s) 143, 207
AvaII GGWCC 2 cut(s) 289, 2596
BaeGI GKGCMC 2 cut(s) 2649, 2842
BaeI ACNNNNGTAYC 2 cut(s) 2836, 2869
BalI TGGCCA 3 cut(s) 236, 1371, 2688
BamHI GGATCC 2 cut(s) 2305, 2905
BanI GGYRCC 1 cut(s) 2837
BbrPI CACGTG 1 cut(s) 1493
BbsI GAAGAC 1 cut(s) 1149
Bbv12I GWGCWC 5 cut(s) 1258, 1300, 1492, 2649, 3232
BbvI GCAGC 7 cut(s) 258, 261, 759, 1951, 2077, 3289, 3295
BccI CCATC 8 cut(s) 355, 623, 754, 1656, 2409, 2678, 2911, 3122
BceAI ACGGC 7 cut(s) 864, 1050, 1167, 1530, 1592, 2899, 3060
BcgI CGANNNNNNTGC 2 cut(s) 3281, 3315
BciT130I CCWGG 6 cut(s) 1581, 1944, 2628, 2690, 2858, 3322
BciVI GTATCC 1 cut(s) 2864
BclI TGATCA 1 cut(s) 2383
BcnI CCSGG 3 cut(s) 1275, 1934, 2303
BcoDI GTCTC 8 cut(s) 21, 321, 809, 1729, 1835, 2526, 2648, 2812
BfaI CTAG 6 cut(s) 725, 857, 1439, 2225, 2358, 2910
BfmI CTRYAG 3 cut(s) 892, 954, 2640
BfoI RGCGCY 3 cut(s) 451, 1409, 3430
BfrI CTTAAG 2 cut(s) 2141, 2527
BfuAI ACCTGC 1 cut(s) 2050
BfuI GTATCC 1 cut(s) 2864
BglII AGATCT 1 cut(s) 1168
Bme1390I CCNGG 9 cut(s) 1275, 1581, 1934, 1944, 2303, 2628, 2690, 2858, 3322
Bme18I GGWCC 2 cut(s) 289, 2596
BmgBI CACGTC 1 cut(s) 1710
BmgT120I GGNCC 2 cut(s) 289, 2596
BmiI GGNNCC 5 cut(s) 411, 2307, 2839, 2907, 3133
BmrFI CCNGG 9 cut(s) 1275, 1581, 1934, 1944, 2303, 2628, 2690, 2858, 3322
BmrI ACTGGG 2 cut(s) 1338, 1614
BmuI ACTGGG 2 cut(s) 1338, 1614
BpiI GAAGAC 1 cut(s) 1149
BplI GAGNNNNNCTC 6 cut(s) 164, 196, 1201, 1233, 1726, 1758
BpmI CTGGAG 2 cut(s) 2479, 3063
Bpu14I TTCGAA 2 cut(s) 143, 207
BpuEI CTTGAG 3 cut(s) 1108, 2307, 3194
BpuMI CCSGG 3 cut(s) 1275, 1934, 2303
BsaAI YACGTR 2 cut(s) 1493, 1754
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 2 cut(s) 1729, 1835
BsaJI CCNNGG 7 cut(s) 231, 880, 1409, 1693, 2626, 2744, 2848
BsaWI WCCGGW 2 cut(s) 732, 2615
Bsc4I CCNNNNNNNGG 6 cut(s) 436, 1658, 2225, 2226, 2745, 2749
Bse1I ACTGG 9 cut(s) 823, 1047, 1289, 1323, 1333, 1609, 2210, 2806, 3321
Bse3DI GCAATG 1 cut(s) 2257
BseAI TCCGGA 2 cut(s) 732, 2615
BseBI CCWGG 6 cut(s) 1581, 1944, 2628, 2690, 2858, 3322
BseDI CCNNGG 7 cut(s) 231, 880, 1409, 1693, 2626, 2744, 2848
BseLI CCNNNNNNNGG 6 cut(s) 436, 1658, 2225, 2226, 2745, 2749
BseMI GCAATG 1 cut(s) 2257
BseMII CTCAG 3 cut(s) 254, 851, 3351
BseNI ACTGG 9 cut(s) 823, 1047, 1289, 1323, 1333, 1609, 2210, 2806, 3321
BseRI GAGGAG 2 cut(s) 31, 1266
BseSI GKGCMC 2 cut(s) 2649, 2842
BseXI GCAGC 7 cut(s) 258, 261, 759, 1951, 2077, 3289, 3295
BseYI CCCAGC 1 cut(s) 2861
BsgI GTGCAG 1 cut(s) 1958
Bsh1236I CGCG 3 cut(s) 150, 2601, 2793
BshFI GGCC 8 cut(s) 236, 879, 1371, 1579, 1932, 2688, 3075, 3320
BshNI GGYRCC 1 cut(s) 2837
BsiHKAI GWGCWC 5 cut(s) 1258, 1300, 1492, 2649, 3232
BsiSI CCGG 6 cut(s) 159, 733, 1274, 1933, 2302, 2616
BslFI GGGAC 2 cut(s) 378, 2359
BslI CCNNNNNNNGG 6 cut(s) 436, 1658, 2225, 2226, 2745, 2749
BsmAI GTCTC 8 cut(s) 21, 321, 809, 1729, 1835, 2526, 2648, 2812
BsmBI CGTCTC 2 cut(s) 21, 2526
BsmFI GGGAC 2 cut(s) 378, 2359
BsmI GAATGC 3 cut(s) 616, 887, 1228
BsnI GGCC 8 cut(s) 236, 879, 1371, 1579, 1932, 2688, 3075, 3320
Bso31I GGTCTC 2 cut(s) 1729, 1835
Bsp119I TTCGAA 2 cut(s) 143, 207
Bsp1286I GDGCHC 6 cut(s) 1258, 1300, 1492, 2649, 2842, 3232
Bsp13I TCCGGA 2 cut(s) 732, 2615
Bsp1407I TGTACA 1 cut(s) 905
Bsp19I CCATGG 2 cut(s) 1693, 2848
BspACI CCGC 9 cut(s) 8, 11, 150, 427, 541, 1226, 1739, 2342, 2599
BspANI GGCC 8 cut(s) 236, 879, 1371, 1579, 1932, 2688, 3075, 3320
BspCNI CTCAG 3 cut(s) 255, 850, 3350
BspEI TCCGGA 2 cut(s) 732, 2615
BspFNI CGCG 3 cut(s) 150, 2601, 2793
BspHI TCATGA 2 cut(s) 1123, 1599
BspLI GGNNCC 5 cut(s) 411, 2307, 2839, 2907, 3133
BspMAI CTGCAG 1 cut(s) 958
BspMI ACCTGC 1 cut(s) 2050
BspQI GCTCTTC 1 cut(s) 2937
BspT104I TTCGAA 2 cut(s) 143, 207
BspT107I GGYRCC 1 cut(s) 2837
BspTI CTTAAG 2 cut(s) 2141, 2527
BspTNI GGTCTC 2 cut(s) 1729, 1835
BsrBI CCGCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 2257
BsrGI TGTACA 1 cut(s) 905
BsrI ACTGG 9 cut(s) 823, 1047, 1289, 1323, 1333, 1609, 2210, 2806, 3321
BssECI CCNNGG 7 cut(s) 231, 880, 1409, 1693, 2626, 2744, 2848
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 3 cut(s) 1693, 2744, 2848
Bst2UI CCWGG 6 cut(s) 1581, 1944, 2628, 2690, 2858, 3322
Bst4CI ACNGT 5 cut(s) 569, 985, 1383, 2782, 3347
Bst6I CTCTTC 5 cut(s) 124, 130, 420, 2508, 2937
BstACI GRCGYC 1 cut(s) 14
BstAFI CTTAAG 2 cut(s) 2141, 2527
BstAUI TGTACA 1 cut(s) 905
BstBAI YACGTR 2 cut(s) 1493, 1754
BstBI TTCGAA 2 cut(s) 143, 207
BstDSI CCRYGG 4 cut(s) 231, 880, 1693, 2848
BstENI CCTNNNNNAGG 1 cut(s) 1656
BstFNI CGCG 3 cut(s) 150, 2601, 2793
BstH2I RGCGCY 3 cut(s) 451, 1409, 3430
BstHHI GCGC 6 cut(s) 150, 450, 672, 1408, 2603, 3429
BstMAI GTCTC 8 cut(s) 21, 321, 809, 1729, 1835, 2526, 2648, 2812
BstMWI GCNNNNNNNGC 6 cut(s) 159, 682, 1405, 2348, 2953, 3311
BstNI CCWGG 6 cut(s) 1581, 1944, 2628, 2690, 2858, 3322
BstNSI RCATGY 5 cut(s) 676, 1106, 1692, 3253, 3394
BstSCI CCNGG 9 cut(s) 1273, 1579, 1932, 1942, 2301, 2626, 2688, 2856, 3320
BstSFI CTRYAG 3 cut(s) 892, 954, 2640
BstSLI GKGCMC 2 cut(s) 2649, 2842
BstSNI TACGTA 1 cut(s) 1754
BstUI CGCG 3 cut(s) 150, 2601, 2793
BstV1I GCAGC 7 cut(s) 258, 261, 759, 1951, 2077, 3289, 3295
BstV2I GAAGAC 1 cut(s) 1149
BstX2I RGATCY 6 cut(s) 1168, 1189, 2215, 2305, 2905, 2977
BstXI CCANNNNNNTGG 1 cut(s) 361
BstYI RGATCY 6 cut(s) 1168, 1189, 2215, 2305, 2905, 2977
BsuI GTATCC 1 cut(s) 2864
BsuRI GGCC 8 cut(s) 236, 879, 1371, 1579, 1932, 2688, 3075, 3320
BtgI CCRYGG 4 cut(s) 231, 880, 1693, 2848
BtrI CACGTC 1 cut(s) 1710
BtsI GCAGTG 1 cut(s) 1431
BtsIMutI CAGTG 7 cut(s) 372, 574, 990, 1054, 1296, 1431, 3314
BveI ACCTGC 1 cut(s) 2050
CaiI CAGNNNCTG 1 cut(s) 1586
CciI TCATGA 2 cut(s) 1123, 1599
CfoI GCGC 6 cut(s) 150, 450, 672, 1408, 2603, 3429
Cfr13I GGNCC 2 cut(s) 289, 2596
CseI GACGC 2 cut(s) 3, 2543
Csp6I GTAC 5 cut(s) 92, 906, 1044, 1316, 1751
CviQI GTAC 5 cut(s) 92, 906, 1044, 1316, 1751
DrdI GACNNNNNNGTC 1 cut(s) 2662
DseDI GACNNNNNNGTC 1 cut(s) 2662
EaeI YGGCCR 7 cut(s) 234, 877, 1369, 1577, 1930, 2686, 3073
Eam1104I CTCTTC 5 cut(s) 124, 130, 420, 2508, 2937
EarI CTCTTC 5 cut(s) 124, 130, 420, 2508, 2937
Eco105I TACGTA 1 cut(s) 1754
Eco130I CCWWGG 3 cut(s) 1693, 2744, 2848
Eco31I GGTCTC 2 cut(s) 1729, 1835
Eco32I GATATC 4 cut(s) 807, 1618, 1893, 2992
Eco47I GGWCC 2 cut(s) 289, 2596
Eco57I CTGAAG 2 cut(s) 638, 729
Eco72I CACGTG 1 cut(s) 1493
EcoNI CCTNNNNNAGG 1 cut(s) 1656
EcoRI GAATTC 2 cut(s) 2029, 2078
EcoRII CCWGG 6 cut(s) 1579, 1942, 2626, 2688, 2856, 3320
EcoRV GATATC 4 cut(s) 807, 1618, 1893, 2992
EcoT14I CCWWGG 3 cut(s) 1693, 2744, 2848
ErhI CCWWGG 3 cut(s) 1693, 2744, 2848
Esp3I CGTCTC 2 cut(s) 21, 2526
FaqI GGGAC 2 cut(s) 378, 2359
FauI CCCGC 1 cut(s) 2335
FauNDI CATATG 4 cut(s) 1849, 2388, 2407, 2609
FbaI TGATCA 1 cut(s) 2383
FblI GTMKAC 1 cut(s) 1876
FspBI CTAG 6 cut(s) 725, 857, 1439, 2225, 2358, 2910
FspI TGCGCA 1 cut(s) 671
GlaI GCGC 6 cut(s) 149, 449, 671, 1407, 2602, 3428
GsaI CCCAGC 1 cut(s) 2865
GsuI CTGGAG 2 cut(s) 2479, 3063
HaeII RGCGCY 3 cut(s) 451, 1409, 3430
HaeIII GGCC 8 cut(s) 236, 879, 1371, 1579, 1932, 2688, 3075, 3320
HapII CCGG 6 cut(s) 159, 733, 1274, 1933, 2302, 2616
HgaI GACGC 2 cut(s) 3, 2543
HhaI GCGC 6 cut(s) 150, 450, 672, 1408, 2603, 3429
Hin1I GRCGYC 1 cut(s) 14
Hin6I GCGC 6 cut(s) 148, 448, 670, 1406, 2601, 3427
HinP1I GCGC 6 cut(s) 148, 448, 670, 1406, 2601, 3427
HincII GTYRAC 2 cut(s) 2776, 3327
HindII GTYRAC 2 cut(s) 2776, 3327
HindIII AAGCTT 2 cut(s) 2933, 2969
HpaII CCGG 6 cut(s) 159, 733, 1274, 1933, 2302, 2616
HphI GGTGA 8 cut(s) 59, 575, 696, 989, 1673, 2771, 3116, 3174
Hpy166II GTNNAC 5 cut(s) 908, 1877, 2647, 2776, 3327
Hpy8I GTNNAC 5 cut(s) 908, 1877, 2647, 2776, 3327
Hpy99I CGWCG 3 cut(s) 188, 191, 1188
HpyCH4III ACNGT 5 cut(s) 569, 985, 1383, 2782, 3347
HpyCH4IV ACGT 6 cut(s) 546, 1492, 1709, 1753, 2722, 3412
HpyF10VI GCNNNNNNNGC 6 cut(s) 159, 682, 1405, 2348, 2953, 3311
HpySE526I ACGT 6 cut(s) 546, 1492, 1709, 1753, 2722, 3412
Hsp92I GRCGYC 1 cut(s) 14
HspAI GCGC 6 cut(s) 148, 448, 670, 1406, 2601, 3427
Kpn2I TCCGGA 2 cut(s) 732, 2615
Ksp22I TGATCA 1 cut(s) 2383
LguI GCTCTTC 1 cut(s) 2937
LmnI GCTCC 4 cut(s) 172, 884, 1228, 1253
Lsp1109I GCAGC 7 cut(s) 258, 261, 759, 1951, 2077, 3289, 3295
MaeI CTAG 6 cut(s) 725, 857, 1439, 2225, 2358, 2910
MaeII ACGT 6 cut(s) 546, 1492, 1709, 1753, 2722, 3412
MaeIII GTNAC 8 cut(s) 281, 563, 569, 1096, 1952, 2579, 2723, 3379
MbiI CCGCTC 1 cut(s) 8
MflI RGATCY 6 cut(s) 1168, 1189, 2215, 2305, 2905, 2977
MhlI GDGCHC 6 cut(s) 1258, 1300, 1492, 2649, 2842, 3232
MlsI TGGCCA 3 cut(s) 236, 1371, 2688
MluNI TGGCCA 3 cut(s) 236, 1371, 2688
MlyI GAGTC 4 cut(s) 869, 2014, 3107, 3322
MmeI TCCRAC 6 cut(s) 69, 121, 207, 1490, 1547, 2859
Mox20I TGGCCA 3 cut(s) 236, 1371, 2688
MroI TCCGGA 2 cut(s) 732, 2615
MscI TGGCCA 3 cut(s) 236, 1371, 2688
MseI TTAA 8 cut(s) 1533, 1641, 2142, 2528, 2565, 2937, 2949, 3439
MslI CAYNNNNRTG 2 cut(s) 1504, 1730
Msp20I TGGCCA 3 cut(s) 236, 1371, 2688
MspA1I CMGCKG 2 cut(s) 1623, 2090
MspCI CTTAAG 2 cut(s) 2141, 2527
MspI CCGG 6 cut(s) 159, 733, 1274, 1933, 2302, 2616
MspR9I CCNGG 9 cut(s) 1275, 1581, 1934, 1944, 2303, 2628, 2690, 2858, 3322
Mva1269I GAATGC 3 cut(s) 616, 887, 1228
MvaI CCWGG 6 cut(s) 1581, 1944, 2628, 2690, 2858, 3322
MvnI CGCG 3 cut(s) 150, 2601, 2793
MwoI GCNNNNNNNGC 6 cut(s) 159, 682, 1405, 2348, 2953, 3311
NciI CCSGG 3 cut(s) 1275, 1934, 2303
NcoI CCATGG 2 cut(s) 1693, 2848
NdeI CATATG 4 cut(s) 1849, 2388, 2407, 2609
NlaIV GGNNCC 5 cut(s) 411, 2307, 2839, 2907, 3133
NmeAIII GCCGAG 1 cut(s) 1888
NmuCI GTSAC 5 cut(s) 281, 563, 569, 2579, 3379
NsbI TGCGCA 1 cut(s) 671
NspI RCATGY 5 cut(s) 676, 1106, 1692, 3253, 3394
NspV TTCGAA 2 cut(s) 143, 207
PaeI GCATGC 2 cut(s) 676, 3253
PagI TCATGA 2 cut(s) 1123, 1599
PciI ACATGT 2 cut(s) 1102, 3390
PciSI GCTCTTC 1 cut(s) 2937
PctI GAATGC 3 cut(s) 616, 887, 1228
PfeI GAWTC 9 cut(s) 617, 701, 760, 872, 1496, 2361, 2795, 2813, 3117
PflFI GACNNNGTC 1 cut(s) 1708
PfoI TCCNGGA 1 cut(s) 2301
PleI GAGTC 4 cut(s) 868, 2014, 3106, 3322
PmaCI CACGTG 1 cut(s) 1493
PmlI CACGTG 1 cut(s) 1493
PpsI GAGTC 4 cut(s) 868, 2014, 3106, 3322
Ppu21I YACGTR 2 cut(s) 1493, 1754
PscI ACATGT 2 cut(s) 1102, 3390
PsiI TTATAA 1 cut(s) 1685
Psp6I CCWGG 6 cut(s) 1579, 1942, 2626, 2688, 2856, 3320
PspCI CACGTG 1 cut(s) 1493
PspFI CCCAGC 1 cut(s) 2861
PspGI CCWGG 6 cut(s) 1579, 1942, 2626, 2688, 2856, 3320
PspN4I GGNNCC 5 cut(s) 411, 2307, 2839, 2907, 3133
PspPI GGNCC 2 cut(s) 289, 2596
PstI CTGCAG 1 cut(s) 958
PstNI CAGNNNCTG 1 cut(s) 1586
PsuI RGATCY 6 cut(s) 1168, 1189, 2215, 2305, 2905, 2977
PsyI GACNNNGTC 1 cut(s) 1708
PvuII CAGCTG 2 cut(s) 1623, 2090
RsaI GTAC 5 cut(s) 93, 907, 1045, 1317, 1752
RsaNI GTAC 5 cut(s) 92, 906, 1044, 1316, 1751
RseI CAYNNNNRTG 2 cut(s) 1504, 1730
SapI GCTCTTC 1 cut(s) 2937
SaqAI TTAA 8 cut(s) 1533, 1641, 2142, 2528, 2565, 2937, 2949, 3439
Sau96I GGNCC 2 cut(s) 289, 2596
SchI GAGTC 4 cut(s) 869, 2014, 3107, 3322
ScrFI CCNGG 9 cut(s) 1275, 1581, 1934, 1944, 2303, 2628, 2690, 2858, 3322
SduI GDGCHC 6 cut(s) 1258, 1300, 1492, 2649, 2842, 3232
SfcI CTRYAG 3 cut(s) 892, 954, 2640
SfuI TTCGAA 2 cut(s) 143, 207
SinI GGWCC 2 cut(s) 289, 2596
SmiMI CAYNNNNRTG 2 cut(s) 1504, 1730
SmlI CTYRAG 5 cut(s) 1087, 2141, 2322, 2527, 3209
SmoI CTYRAG 5 cut(s) 1087, 2141, 2322, 2527, 3209
SnaBI TACGTA 1 cut(s) 1754
SphI GCATGC 2 cut(s) 676, 3253
SsiI CCGC 9 cut(s) 8, 11, 150, 427, 541, 1226, 1739, 2342, 2599
SspI AATATT 1 cut(s) 2195
SspMI CTAG 6 cut(s) 725, 857, 1439, 2225, 2358, 2910
StyD4I CCNGG 9 cut(s) 1273, 1579, 1932, 1942, 2301, 2626, 2688, 2856, 3320
StyI CCWWGG 3 cut(s) 1693, 2744, 2848
TaaI ACNGT 5 cut(s) 569, 985, 1383, 2782, 3347
TaiI ACGT 6 cut(s) 549, 1495, 1712, 1756, 2725, 3415
TaqI TCGA 7 cut(s) 137, 143, 207, 579, 916, 1000, 2988
TatI WGTACW 3 cut(s) 91, 905, 1315
TauI GCSGC 3 cut(s) 11, 14, 153
TfiI GAWTC 9 cut(s) 617, 701, 760, 872, 1496, 2361, 2795, 2813, 3117
Tru1I TTAA 8 cut(s) 1533, 1641, 2142, 2528, 2565, 2937, 2949, 3439
Tru9I TTAA 8 cut(s) 1533, 1641, 2142, 2528, 2565, 2937, 2949, 3439
TscAI CASTG 7 cut(s) 379, 574, 990, 1054, 1296, 1438, 3321
TseFI GTSAC 5 cut(s) 281, 563, 569, 2579, 3379
TseI GCWGC 7 cut(s) 271, 274, 772, 1939, 2090, 3283, 3302
Tsp45I GTSAC 5 cut(s) 281, 563, 569, 2579, 3379
TspGWI ACGGA 2 cut(s) 220, 373
TspRI CASTG 7 cut(s) 379, 574, 990, 1054, 1296, 1438, 3321
Tth111I GACNNNGTC 1 cut(s) 1708
Vha464I CTTAAG 2 cut(s) 2141, 2527
VneI GTGCAC 1 cut(s) 2645
VpaK11BI GGWCC 2 cut(s) 289, 2596
XagI CCTNNNNNAGG 1 cut(s) 1656
XapI RAATTY 7 cut(s) 77, 1119, 1240, 2029, 2078, 2173, 2464
XbaI TCTAGA 1 cut(s) 2357
XceI RCATGY 5 cut(s) 676, 1106, 1692, 3253, 3394
XcmI CCANNNNNNNNNTGG 1 cut(s) 3313
XmiI GTMKAC 1 cut(s) 1876
XspI CTAG 6 cut(s) 725, 857, 1439, 2225, 2358, 2910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.