Rroxscaffold_5G00350810

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005499
Physical Location & Seq
Forward (+)
25404039 .. 25419000
14962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00350810.1

Sequence Viewer

Length: 3483 bp
ATGAGAGCGGCGGCGTCTCTCCTCGTCAAATCAGTAGCCTCCGCCAACAATACTTGTTTTCGTTCTCACCACCTCAAATTCTGTCTTGTTGTACTCCAACCACAATCACAACCACACTCTTCTTCTTCTTCTTCCTCTTCTCCCATTTCGATTTCGAAGCGCGGCTTTTCCGGCTACGCTGCGGAGCAGTTCTCCGACGACGAGTATGAGTGCGATTTCGAAAGCCAAAAGGCTTCATCTTCCGTGGCCAACATTGACGAGTGGAAATGGAAACTGAGCCTGCTGCTGCGCAGTGACAAGGACCAAGAGATTGTTTCCAGAGATAAAAGGGACAGGAGAGACTATGAGCAGATATCTAACCTCGCCAAACGGATGGGACTTTACACTGAAATATATGGCAAAGTGGTGGTTGCCAGCAAGGTTCCTCTTCCAAACTACCGCCCCGATTTGGATGATAAGCGCCCACAAAGAGAGGTGGTTATCCCTCTTGGCTTGCAAAGGAGGGTAGAGGGTTTACTGCAAGAACACCTTGATCGTGTCCTGCTAAGTTCCCGCAACCTATCAGCCAATTCAGGTGACAGTGAGCACTTCGATCAGGTTGAAAATGAAAAGCCTGATGCGATTGCAGATTCTTTTCTTGATGGGTCTGTAATGGAGAAGGTTCTTCAGCGTCGGAGTTTGCGCATGCGTAATATGCAAAGAGCTTGGCAGGAATCACCTGAAGGTAAAAAGATGCTAGAGTTCCGGAAATCACTACCATCTTTCAAAGAGAATCAGAGGTTGCTGCAAGCAATAGCACAAAATCAGGTAGTAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAGCTTCCTCAATATATACTAGAGTCAGAGATAGAATCTGGCCGTGGAGCATTCTGTAGCATTATCTGTACACAACCTCGAAGAATATCTGCTATGGCTGTTGCTGAAAGAGTGTCTGCAGAGAGGGGAGAGCCTCTTGGTGAAACAGTGGGCTATAAAGTTCGATTAGAGGGAATGAAAGGAAAAAATACGCATCTCCTTTTCTGTACCAGTGGTATTCTACTTCGGCGTCTGTTGAGTGATCGGAACTTGAGTGGTGTAACACATGTTTTTGTTGATGAAATTCATGAACGAGGCATGAATGAAGGTGCACTTCTTTACATACTTCTTGTTGATTGTATTGAAAGATCTGCTTCCACGCCGTCGGGATCTAAGATTGGTTTTGATGAGTGCCACTCTGAATGCGGAGCTATTTTCAAATTTTTTGGACGAGCACCAACAATTCACATTCCGATTAGAAATGGGGATATCAATACTAGAGAGCTAGATGTGGAGATAAAGAGTAGTTTTAATCTATGCAGTGTTATTTTAACTGATTGTAAATGTTATGCTGTGGAAGTCATAGGTTTCACATATCCAGTGAGGGCACACTTTTTGGAAGATGTACTGGAAATGACTGGGTATAAGCTGACTTCTTTCAACCAAATTGATGACTATGGCCAAGACAAACTGTGGAAAACGCAGAAGCAGTTAGCGCCTCGGAAAAGGAAAAACCAGATCACTGCTCTAGTTGAGATTGTCCCATATATTGTTAAGGATGCTCTTAGTAAATCAAGTTTTGAGAATTATAGCCCCAGAGCACGCGATTCATTATCATGTTGGACGGCTGATTGCATAGGATTTAATCTTATTGAAGCAGTTTTATGTCATATATGTCGGAAGGAACGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGAGGATATCAGCTGTTTGAGGGATCAACTTAAAGCCCATCCTCTTTTAGGTGATCCTAACAGGGTTTTGCTTATAACATGCCATGGGTCAATGACCACGTCTGAACAGAGAAGGGGAAGGGCTGGCCGGGTGCAGCCAGGCGAATGTTACCACCTTTACCCCAAATGTGTGTATCATGCGTTTGCAGAATACCAACTTCCTGAACTCCTAAGGACTCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTTTGCAGGTTGGTAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCACCTGAACCCCTTGCTATTTCTGTCACTCTGTTGCGTTGTGATATTAATTCTTGGAAACCCACAATGTGGTCCCTTTTGCCTGATGTGAGAATCCTGGTTCAAAATGCTGTTGGCTTTCTTAAGTTGATTGGGGCATTGGATGAGAATGAAAATTTGACAAATCTCGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTAAGTGTCCGGGATCCATTCCTTTTGCCTCAAGACAAAAAAGATTTAGCGGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTATAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCAGAAAGAGAAGGATCAGCATATGAATACTGCTGGAGAAATTTTCTCTCTGCCCAGACACTTCAAGCTATTCATTCTCTTCGGAAGCAGTTTAGCTACATCTTAAGAGACGCTGGCTTGGTGGATGCAGATGCAAGCATTAACAACAAGCTAAGTCACAATCAGTCATTGGTCCGCGCAATCATATGTTCCGGACTATTCCCTGGCATAGCATCTGTACACCGAGAGACATCCATGTCTTTCAAAACTATGGATGATGGCCAGGTTTTACTATATGCTAATTCTGTAAATGCACGTTACCAAACAATTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAGTAAAGGTCAACACAGTTTTCATACGTGATTCTACTGGTGTCTCTGATTCCATTTTGATCCTTTTTGGTGGTGCCCTAAACCATGGTATCCAGGCTGGGCATTTGAAAATGTTGGACGGCTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGCTTAAAGAAGAGCTTAATGAGCTTATTCAGAAGAAGCTTCAAGATCCAAGCCTCGATATCCACAAGGAAGGAAAGTATCTCATACTTGCTGTTCAAGAGCTGGTATCTGGAGATCAAAGTGAAGGAAGATTTGTGTTTGGCCGTGAAAACAGGAAGCCAAAGGAGTCTGGTGATAACAGCAGATTCACAAGGGATGGAACCAATCCTAAGAGTTTGCTACAAACTCTATTGATGAGAGCAGGACATTCACCTCCAAAATACAAAACCAAGCACCTCAAGACAAATGAGTTCAGAGCACTTGTGGAGTTCAAGGGCATGCAATTTGTTGGAAAGCCCAAGAAAAATAAGCAGCTTGCCGAAAGGGATGCTGCCATAGAAGCACTGGCCTGGTTGACTCACACCTCAGACAACAGTCGTGATGAAGATGACAACTCCCCACCTGATGTCACTAACAACATGCTGAAACTTCTGGGGAAACGTAGAAGATCAAAGCGCCACTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1160

Amino Acids

130.54

Weight (kDa)

8.61

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 260 - 381 4.4e-06 DEAD/DEAH box helicase
WHD_HA2 PF04408 725 - 753 2.9e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 754 - 814 6e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 881 - 961 9e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 970 - 1028 2.6e-23 C-terminal domain in RNA helicases
dsrm PF00035 1061 - 1123 7.8e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1844
AasI GACNNNNNNGTC 1 cut(s) 2704
Acc16I TGCGCA 2 cut(s) 290, 683
Acc36I ACCTGC 1 cut(s) 2014
AccB1I GGYRCC 1 cut(s) 2879
AccB7I CCANNNNNTGG 1 cut(s) 2133
AccBSI CCGCTC 1 cut(s) 8
AccII CGCG 3 cut(s) 162, 1653, 2646
AccIII TCCGGA 2 cut(s) 744, 2660
AcoI YGGCCR 7 cut(s) 246, 889, 1507, 1736, 1894, 2728, 3115
AcsI RAATTY 7 cut(s) 77, 1131, 1268, 1993, 2042, 2218, 2509
AcuI CTGAAG 2 cut(s) 650, 741
AcyI GRCGYC 2 cut(s) 14, 1078
AdeI CACNNNGTG 1 cut(s) 2133
AfaI GTAC 5 cut(s) 93, 919, 1057, 1455, 2688
AfiI CCNNNNNNNGG 7 cut(s) 448, 1817, 2133, 2270, 2271, 2787, 2791
AflII CTTAAG 2 cut(s) 2186, 2572
AflIII ACRYGT 1 cut(s) 1114
AjiI CACGTC 1 cut(s) 1869
AjnI CCWGG 7 cut(s) 1738, 1906, 2160, 2671, 2730, 2898, 3362
Alw21I GWGCWC 5 cut(s) 588, 1162, 1285, 1651, 3274
Alw26I GTCTC 6 cut(s) 21, 333, 821, 2571, 2690, 2854
Alw44I GTGCAC 1 cut(s) 1158
AlwNI CAGNNNCTG 1 cut(s) 1745
Aor13HI TCCGGA 2 cut(s) 744, 2660
AoxI GGCC 8 cut(s) 246, 889, 1507, 1736, 1894, 2728, 3115, 3360
ApaLI GTGCAC 1 cut(s) 1158
ApeKI GCWGC 8 cut(s) 179, 283, 286, 784, 1903, 2054, 3325, 3344
ApoI RAATTY 7 cut(s) 77, 1131, 1268, 1993, 2042, 2218, 2509
AseI ATTAAT 1 cut(s) 2112
AspLEI GCGC 7 cut(s) 162, 291, 462, 684, 1546, 2648, 3471
AspS9I GGNCC 3 cut(s) 301, 2136, 2641
AsuC2I CCSGG 2 cut(s) 1898, 2348
AsuHPI GGTGA 8 cut(s) 59, 587, 708, 1001, 1832, 2813, 3158, 3216
AsuII TTCGAA 2 cut(s) 155, 219
AvaII GGWCC 3 cut(s) 301, 2136, 2641
AxyI CCTNAGG 1 cut(s) 1979
BaeGI GKGCMC 3 cut(s) 1162, 1438, 2884
BaeI ACNNNNGTAYC 2 cut(s) 2878, 2911
BalI TGGCCA 3 cut(s) 248, 1509, 2730
BamHI GGATCC 2 cut(s) 2350, 2947
BanI GGYRCC 1 cut(s) 2879
Bbv12I GWGCWC 5 cut(s) 588, 1162, 1285, 1651, 3274
BbvI GCAGC 8 cut(s) 166, 270, 273, 771, 1915, 2041, 3331, 3337
BccI CCATC 8 cut(s) 367, 635, 766, 1815, 2454, 2720, 2953, 3164
BceAI ACGGC 6 cut(s) 876, 1195, 1689, 1751, 2941, 3102
BcgI CGANNNNNNTGC 2 cut(s) 3323, 3357
BciT130I CCWGG 7 cut(s) 1740, 1908, 2162, 2673, 2732, 2900, 3364
BciVI GTATCC 1 cut(s) 2906
BclI TGATCA 1 cut(s) 2428
BcnI CCSGG 2 cut(s) 1898, 2348
BcoDI GTCTC 6 cut(s) 21, 333, 821, 2571, 2690, 2854
BfaI CTAG 8 cut(s) 737, 869, 1326, 1334, 1577, 2270, 2403, 2952
BfmI CTRYAG 2 cut(s) 904, 966
BfoI RGCGCY 3 cut(s) 463, 1547, 3472
BfrI CTTAAG 2 cut(s) 2186, 2572
BfuAI ACCTGC 1 cut(s) 2014
BfuI GTATCC 1 cut(s) 2906
BglII AGATCT 1 cut(s) 1196
Bme1390I CCNGG 9 cut(s) 1740, 1898, 1908, 2162, 2348, 2673, 2732, 2900, 3364
Bme18I GGWCC 3 cut(s) 301, 2136, 2641
BmgBI CACGTC 1 cut(s) 1869
BmgT120I GGNCC 3 cut(s) 301, 2136, 2641
BmiI GGNNCC 6 cut(s) 423, 2138, 2352, 2881, 2949, 3175
BmrFI CCNGG 9 cut(s) 1740, 1898, 1908, 2162, 2348, 2673, 2732, 2900, 3364
BmrI ACTGGG 2 cut(s) 1476, 1773
BmsI GCATC 9 cut(s) 607, 723, 1051, 1597, 2458, 2584, 2590, 2690, 3331
BmuI ACTGGG 2 cut(s) 1476, 1773
BoxI GACNNNNGTC 1 cut(s) 3387
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 1229, 1261
BpmI CTGGAG 2 cut(s) 2524, 3105
Bpu14I TTCGAA 2 cut(s) 155, 219
BpuEI CTTGAG 3 cut(s) 1120, 2352, 3236
BpuMI CCSGG 2 cut(s) 1898, 2348
BsaAI YACGTR 1 cut(s) 2834
BsaHI GRCGYC 2 cut(s) 14, 1078
BsaJI CCNNGG 7 cut(s) 243, 892, 1547, 1852, 2671, 2786, 2890
BsaWI WCCGGW 2 cut(s) 744, 2660
Bsc4I CCNNNNNNNGG 7 cut(s) 448, 1817, 2133, 2270, 2271, 2787, 2791
Bse1I ACTGG 9 cut(s) 835, 1059, 1427, 1461, 1471, 1768, 2255, 2848, 3363
Bse21I CCTNAGG 1 cut(s) 1979
Bse3DI GCAATG 1 cut(s) 2302
BseAI TCCGGA 2 cut(s) 744, 2660
BseBI CCWGG 7 cut(s) 1740, 1908, 2162, 2673, 2732, 2900, 3364
BseDI CCNNGG 7 cut(s) 243, 892, 1547, 1852, 2671, 2786, 2890
BseLI CCNNNNNNNGG 7 cut(s) 448, 1817, 2133, 2270, 2271, 2787, 2791
BseMI GCAATG 1 cut(s) 2302
BseMII CTCAG 3 cut(s) 266, 863, 3393
BseNI ACTGG 9 cut(s) 835, 1059, 1427, 1461, 1471, 1768, 2255, 2848, 3363
BseRI GAGGAG 1 cut(s) 11
BseSI GKGCMC 3 cut(s) 1162, 1438, 2884
BseXI GCAGC 8 cut(s) 166, 270, 273, 771, 1915, 2041, 3331, 3337
BseYI CCCAGC 1 cut(s) 2903
BsgI GTGCAG 1 cut(s) 1922
Bsh1236I CGCG 3 cut(s) 162, 1653, 2646
BshFI GGCC 8 cut(s) 248, 891, 1509, 1738, 1896, 2730, 3117, 3362
BshNI GGYRCC 1 cut(s) 2879
BsiHKAI GWGCWC 5 cut(s) 588, 1162, 1285, 1651, 3274
BsiSI CCGG 5 cut(s) 171, 745, 1897, 2347, 2661
BslFI GGGAC 5 cut(s) 344, 390, 1574, 2122, 2404
BslI CCNNNNNNNGG 7 cut(s) 448, 1817, 2133, 2270, 2271, 2787, 2791
BsmAI GTCTC 6 cut(s) 21, 333, 821, 2571, 2690, 2854
BsmBI CGTCTC 2 cut(s) 21, 2571
BsmFI GGGAC 5 cut(s) 344, 390, 1574, 2122, 2404
BsmI GAATGC 2 cut(s) 899, 1256
BsnI GGCC 8 cut(s) 248, 891, 1509, 1738, 1896, 2730, 3117, 3362
Bsp119I TTCGAA 2 cut(s) 155, 219
Bsp1286I GDGCHC 7 cut(s) 588, 1162, 1285, 1438, 1651, 2884, 3274
Bsp13I TCCGGA 2 cut(s) 744, 2660
Bsp1407I TGTACA 2 cut(s) 917, 2686
Bsp19I CCATGG 2 cut(s) 1852, 2890
BspANI GGCC 8 cut(s) 248, 891, 1509, 1738, 1896, 2730, 3117, 3362
BspCNI CTCAG 3 cut(s) 267, 862, 3392
BspEI TCCGGA 2 cut(s) 744, 2660
BspFNI CGCG 3 cut(s) 162, 1653, 2646
BspHI TCATGA 2 cut(s) 1135, 1758
BspLI GGNNCC 6 cut(s) 423, 2138, 2352, 2881, 2949, 3175
BspMAI CTGCAG 1 cut(s) 970
BspMI ACCTGC 1 cut(s) 2014
BspQI GCTCTTC 1 cut(s) 2979
BspT104I TTCGAA 2 cut(s) 155, 219
BspT107I GGYRCC 1 cut(s) 2879
BspTI CTTAAG 2 cut(s) 2186, 2572
BsrBI CCGCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 2302
BsrGI TGTACA 2 cut(s) 917, 2686
BsrI ACTGG 9 cut(s) 835, 1059, 1427, 1461, 1471, 1768, 2255, 2848, 3363
BssECI CCNNGG 7 cut(s) 243, 892, 1547, 1852, 2671, 2786, 2890
BssNI GRCGYC 2 cut(s) 14, 1078
BssT1I CCWWGG 3 cut(s) 1852, 2786, 2890
Bst2UI CCWGG 7 cut(s) 1740, 1908, 2162, 2673, 2732, 2900, 3364
Bst4CI ACNGT 5 cut(s) 581, 997, 1521, 2824, 3389
Bst6I CTCTTC 5 cut(s) 124, 142, 432, 2553, 2979
BstACI GRCGYC 2 cut(s) 14, 1078
BstAFI CTTAAG 2 cut(s) 2186, 2572
BstAUI TGTACA 2 cut(s) 917, 2686
BstBAI YACGTR 1 cut(s) 2834
BstBI TTCGAA 2 cut(s) 155, 219
BstDSI CCRYGG 4 cut(s) 243, 892, 1852, 2890
BstENI CCTNNNNNAGG 1 cut(s) 1815
BstFNI CGCG 3 cut(s) 162, 1653, 2646
BstH2I RGCGCY 3 cut(s) 463, 1547, 3472
BstHHI GCGC 7 cut(s) 162, 291, 462, 684, 1546, 2648, 3471
BstMAI GTCTC 6 cut(s) 21, 333, 821, 2571, 2690, 2854
BstMWI GCNNNNNNNGC 6 cut(s) 171, 694, 1543, 2393, 2995, 3353
BstNI CCWGG 7 cut(s) 1740, 1908, 2162, 2673, 2732, 2900, 3364
BstNSI RCATGY 5 cut(s) 688, 1118, 1851, 3295, 3436
BstPAI GACNNNNGTC 1 cut(s) 3387
BstSCI CCNGG 9 cut(s) 1738, 1896, 1906, 2160, 2346, 2671, 2730, 2898, 3362
BstSFI CTRYAG 2 cut(s) 904, 966
BstSLI GKGCMC 3 cut(s) 1162, 1438, 2884
BstUI CGCG 3 cut(s) 162, 1653, 2646
BstV1I GCAGC 8 cut(s) 166, 270, 273, 771, 1915, 2041, 3331, 3337
BstX2I RGATCY 6 cut(s) 1196, 1217, 2260, 2350, 2947, 3019
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 6 cut(s) 1196, 1217, 2260, 2350, 2947, 3019
Bsu36I CCTNAGG 1 cut(s) 1979
BsuI GTATCC 1 cut(s) 2906
BsuRI GGCC 8 cut(s) 248, 891, 1509, 1738, 1896, 2730, 3117, 3362
BtgI CCRYGG 4 cut(s) 243, 892, 1852, 2890
BtrI CACGTC 1 cut(s) 1869
BtsI GCAGTG 3 cut(s) 298, 1375, 1569
BtsIMutI CAGTG 9 cut(s) 298, 384, 586, 1002, 1066, 1375, 1434, 1569, 3356
BveI ACCTGC 1 cut(s) 2014
CaiI CAGNNNCTG 1 cut(s) 1745
CciI TCATGA 2 cut(s) 1135, 1758
CfoI GCGC 7 cut(s) 162, 291, 462, 684, 1546, 2648, 3471
Cfr13I GGNCC 3 cut(s) 301, 2136, 2641
CseI GACGC 4 cut(s) 3, 659, 1067, 2588
Csp6I GTAC 5 cut(s) 92, 918, 1056, 1454, 2687
CviQI GTAC 5 cut(s) 92, 918, 1056, 1454, 2687
DraIII CACNNNGTG 1 cut(s) 2133
DrdI GACNNNNNNGTC 1 cut(s) 2704
DseDI GACNNNNNNGTC 1 cut(s) 2704
EaeI YGGCCR 7 cut(s) 246, 889, 1507, 1736, 1894, 2728, 3115
Eam1104I CTCTTC 5 cut(s) 124, 142, 432, 2553, 2979
EarI CTCTTC 5 cut(s) 124, 142, 432, 2553, 2979
EciI GGCGGA 1 cut(s) 31
Eco130I CCWWGG 3 cut(s) 1852, 2786, 2890
Eco32I GATATC 5 cut(s) 354, 819, 1318, 1777, 3034
Eco47I GGWCC 3 cut(s) 301, 2136, 2641
Eco57I CTGAAG 2 cut(s) 650, 741
Eco81I CCTNAGG 1 cut(s) 1979
EcoNI CCTNNNNNAGG 1 cut(s) 1815
EcoRI GAATTC 2 cut(s) 1993, 2042
EcoRII CCWGG 7 cut(s) 1738, 1906, 2160, 2671, 2730, 2898, 3362
EcoRV GATATC 5 cut(s) 354, 819, 1318, 1777, 3034
EcoT14I CCWWGG 3 cut(s) 1852, 2786, 2890
ErhI CCWWGG 3 cut(s) 1852, 2786, 2890
Esp3I CGTCTC 2 cut(s) 21, 2571
FaqI GGGAC 5 cut(s) 344, 390, 1574, 2122, 2404
FauI CCCGC 2 cut(s) 560, 2380
FauNDI CATATG 4 cut(s) 2433, 2452, 2491, 2654
FbaI TGATCA 1 cut(s) 2428
FspBI CTAG 8 cut(s) 737, 869, 1326, 1334, 1577, 2270, 2403, 2952
FspI TGCGCA 2 cut(s) 290, 683
GlaI GCGC 7 cut(s) 161, 290, 461, 683, 1545, 2647, 3470
GsaI CCCAGC 1 cut(s) 2907
GsuI CTGGAG 2 cut(s) 2524, 3105
HaeII RGCGCY 3 cut(s) 463, 1547, 3472
HaeIII GGCC 8 cut(s) 248, 891, 1509, 1738, 1896, 2730, 3117, 3362
HapII CCGG 5 cut(s) 171, 745, 1897, 2347, 2661
HgaI GACGC 4 cut(s) 3, 659, 1067, 2588
HhaI GCGC 7 cut(s) 162, 291, 462, 684, 1546, 2648, 3471
Hin1I GRCGYC 2 cut(s) 14, 1078
Hin6I GCGC 7 cut(s) 160, 289, 460, 682, 1544, 2646, 3469
HinP1I GCGC 7 cut(s) 160, 289, 460, 682, 1544, 2646, 3469
HincII GTYRAC 2 cut(s) 2818, 3369
HindII GTYRAC 2 cut(s) 2818, 3369
HindIII AAGCTT 2 cut(s) 2975, 3011
HpaII CCGG 5 cut(s) 171, 745, 1897, 2347, 2661
HphI GGTGA 8 cut(s) 59, 587, 708, 1001, 1832, 2813, 3158, 3216
Hpy166II GTNNAC 6 cut(s) 515, 920, 1160, 2689, 2818, 3369
Hpy8I GTNNAC 6 cut(s) 515, 920, 1160, 2689, 2818, 3369
Hpy99I CGWCG 4 cut(s) 200, 203, 675, 1216
HpyCH4III ACNGT 5 cut(s) 581, 997, 1521, 2824, 3389
HpyCH4IV ACGT 4 cut(s) 1868, 2764, 2833, 3454
HpyF10VI GCNNNNNNNGC 6 cut(s) 171, 694, 1543, 2393, 2995, 3353
HpySE526I ACGT 4 cut(s) 1868, 2764, 2833, 3454
Hsp92I GRCGYC 2 cut(s) 14, 1078
HspAI GCGC 7 cut(s) 160, 289, 460, 682, 1544, 2646, 3469
Kpn2I TCCGGA 2 cut(s) 744, 2660
Ksp22I TGATCA 1 cut(s) 2428
LguI GCTCTTC 1 cut(s) 2979
LmnI GCTCC 3 cut(s) 184, 896, 1256
Lsp1109I GCAGC 8 cut(s) 166, 270, 273, 771, 1915, 2041, 3331, 3337
LweI GCATC 9 cut(s) 607, 723, 1051, 1597, 2458, 2584, 2590, 2690, 3331
MaeI CTAG 8 cut(s) 737, 869, 1326, 1334, 1577, 2270, 2403, 2952
MaeII ACGT 4 cut(s) 1868, 2764, 2833, 3454
MaeIII GTNAC 8 cut(s) 293, 575, 1108, 1916, 2089, 2624, 2765, 3421
MbiI CCGCTC 1 cut(s) 8
MflI RGATCY 6 cut(s) 1196, 1217, 2260, 2350, 2947, 3019
MhlI GDGCHC 7 cut(s) 588, 1162, 1285, 1438, 1651, 2884, 3274
MlsI TGGCCA 3 cut(s) 248, 1509, 2730
MluNI TGGCCA 3 cut(s) 248, 1509, 2730
MlyI GAGTC 4 cut(s) 881, 1978, 3149, 3364
MmeI TCCRAC 7 cut(s) 121, 219, 653, 1649, 1706, 2901, 3283
Mox20I TGGCCA 3 cut(s) 248, 1509, 2730
MroI TCCGGA 2 cut(s) 744, 2660
MscI TGGCCA 3 cut(s) 248, 1509, 2730
MslI CAYNNNNRTG 1 cut(s) 1663
Msp20I TGGCCA 3 cut(s) 248, 1509, 2730
MspA1I CMGCKG 2 cut(s) 1782, 2054
MspCI CTTAAG 2 cut(s) 2186, 2572
MspI CCGG 5 cut(s) 171, 745, 1897, 2347, 2661
MspR9I CCNGG 9 cut(s) 1740, 1898, 1908, 2162, 2348, 2673, 2732, 2900, 3364
Mva1269I GAATGC 2 cut(s) 899, 1256
MvaI CCWGG 7 cut(s) 1740, 1908, 2162, 2673, 2732, 2900, 3364
MvnI CGCG 3 cut(s) 162, 1653, 2646
MwoI GCNNNNNNNGC 6 cut(s) 171, 694, 1543, 2393, 2995, 3353
NciI CCSGG 2 cut(s) 1898, 2348
NcoI CCATGG 2 cut(s) 1852, 2890
NdeI CATATG 4 cut(s) 2433, 2452, 2491, 2654
NlaIV GGNNCC 6 cut(s) 423, 2138, 2352, 2881, 2949, 3175
NmuCI GTSAC 5 cut(s) 293, 575, 2089, 2624, 3421
NsbI TGCGCA 2 cut(s) 290, 683
NspI RCATGY 5 cut(s) 688, 1118, 1851, 3295, 3436
NspV TTCGAA 2 cut(s) 155, 219
PaeI GCATGC 2 cut(s) 688, 3295
PagI TCATGA 2 cut(s) 1135, 1758
PciI ACATGT 1 cut(s) 1114
PciSI GCTCTTC 1 cut(s) 2979
PctI GAATGC 2 cut(s) 899, 1256
PflFI GACNNNGTC 1 cut(s) 1867
PflMI CCANNNNNTGG 1 cut(s) 2133
PfoI TCCNGGA 1 cut(s) 2346
PleI GAGTC 4 cut(s) 880, 1978, 3148, 3364
PpsI GAGTC 4 cut(s) 880, 1978, 3148, 3364
Ppu21I YACGTR 1 cut(s) 2834
PscI ACATGT 1 cut(s) 1114
PshAI GACNNNNGTC 1 cut(s) 3387
PshBI ATTAAT 1 cut(s) 2112
PsiI TTATAA 1 cut(s) 1844
Psp6I CCWGG 7 cut(s) 1738, 1906, 2160, 2671, 2730, 2898, 3362
PspFI CCCAGC 1 cut(s) 2903
PspGI CCWGG 7 cut(s) 1738, 1906, 2160, 2671, 2730, 2898, 3362
PspN4I GGNNCC 6 cut(s) 423, 2138, 2352, 2881, 2949, 3175
PspPI GGNCC 3 cut(s) 301, 2136, 2641
PstI CTGCAG 1 cut(s) 970
PstNI CAGNNNCTG 1 cut(s) 1745
PsuI RGATCY 6 cut(s) 1196, 1217, 2260, 2350, 2947, 3019
PsyI GACNNNGTC 1 cut(s) 1867
PvuII CAGCTG 2 cut(s) 1782, 2054
RsaI GTAC 5 cut(s) 93, 919, 1057, 1455, 2688
RsaNI GTAC 5 cut(s) 92, 918, 1056, 1454, 2687
RseI CAYNNNNRTG 1 cut(s) 1663
SapI GCTCTTC 1 cut(s) 2979
Sau96I GGNCC 3 cut(s) 301, 2136, 2641
SchI GAGTC 4 cut(s) 881, 1978, 3149, 3364
ScrFI CCNGG 9 cut(s) 1740, 1898, 1908, 2162, 2348, 2673, 2732, 2900, 3364
SduI GDGCHC 7 cut(s) 588, 1162, 1285, 1438, 1651, 2884, 3274
SfaNI GCATC 9 cut(s) 607, 723, 1051, 1597, 2458, 2584, 2590, 2690, 3331
SfcI CTRYAG 2 cut(s) 904, 966
SfuI TTCGAA 2 cut(s) 155, 219
SinI GGWCC 3 cut(s) 301, 2136, 2641
SmiMI CAYNNNNRTG 1 cut(s) 1663
SmlI CTYRAG 5 cut(s) 1099, 2186, 2367, 2572, 3251
SmoI CTYRAG 5 cut(s) 1099, 2186, 2367, 2572, 3251
SphI GCATGC 2 cut(s) 688, 3295
SspI AATATT 1 cut(s) 2240
SspMI CTAG 8 cut(s) 737, 869, 1326, 1334, 1577, 2270, 2403, 2952
StyD4I CCNGG 9 cut(s) 1738, 1896, 1906, 2160, 2346, 2671, 2730, 2898, 3362
StyI CCWWGG 3 cut(s) 1852, 2786, 2890
TaaI ACNGT 5 cut(s) 581, 997, 1521, 2824, 3389
TaiI ACGT 4 cut(s) 1871, 2767, 2836, 3457
TaqI TCGA 7 cut(s) 149, 155, 219, 591, 928, 1012, 3030
TatI WGTACW 4 cut(s) 91, 917, 1453, 2686
TauI GCSGC 3 cut(s) 11, 14, 165
TscAI CASTG 9 cut(s) 298, 391, 586, 1002, 1066, 1375, 1434, 1576, 3363
TseFI GTSAC 5 cut(s) 293, 575, 2089, 2624, 3421
TseI GCWGC 8 cut(s) 179, 283, 286, 784, 1903, 2054, 3325, 3344
Tsp45I GTSAC 5 cut(s) 293, 575, 2089, 2624, 3421
TspGWI ACGGA 2 cut(s) 232, 385
TspRI CASTG 9 cut(s) 298, 391, 586, 1002, 1066, 1375, 1434, 1576, 3363
Tth111I GACNNNGTC 1 cut(s) 1867
Van91I CCANNNNNTGG 1 cut(s) 2133
Vha464I CTTAAG 2 cut(s) 2186, 2572
VneI GTGCAC 1 cut(s) 1158
VpaK11BI GGWCC 3 cut(s) 301, 2136, 2641
VspI ATTAAT 1 cut(s) 2112
XagI CCTNNNNNAGG 1 cut(s) 1815
XapI RAATTY 7 cut(s) 77, 1131, 1268, 1993, 2042, 2218, 2509
XbaI TCTAGA 1 cut(s) 2402
XceI RCATGY 5 cut(s) 688, 1118, 1851, 3295, 3436
XcmI CCANNNNNNNNNTGG 1 cut(s) 3355
XspI CTAG 8 cut(s) 737, 869, 1326, 1334, 1577, 2270, 2403, 2952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.