RLG00000008798

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005499
Physical Location & Seq
Reverse (-)
39365588 .. 39381204
15617 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008798

Sequence Viewer

Length: 3081 bp
ATGGGACTTTACACTGAAATATATGGCAAAGTGGTGGTTGCCAGCAAGGTTCCTCTTCCAAACTACCGCCCCGATTTGGATGACAAGCGCCCACAAAGAGAGGTGGTTATCCCTCTTGGCTTGCAAAGGAGGGTAGAGGGTTTACTGCAAGAACACCTTGATCGTGTCCTGCTAAGTTTCCGCAACGTATCAGCCAATTCAGGTGACAGTGACCACTTCGATCAGGTTGAAAATGAAAAGCCTGATGCGAATGCAGATTCTTTTCTTGATGGGTCTGTAATGGAGAAGGTTCTTCAGCGTAGGAGTTTGCGCATACGTAACATGCAAAGAGCTTGGCAGGAATCACCTGAAGGTAAAAAGATGCTAGAGTTCCGGAAATCACTACCATCTTTCAAAGAGAATCAGAGGCTGCTACAAGCAATAGCACAAAATCAGGTAGTAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAGCTTCCTCAATATATACTAGAGTCAGAGATAGAATCTGGCCGTGGAGCATTCTGTAGCATTATCTGTACACAACCTCGAAGAATATCTGCTATGGCTGTTGCTGAAAGAGTGTCTGCAGAGAGGGGAGAGCCTCTTGGTGAAACAGTGGGCTATAAAGTTCGATTAGAGGGAATGAAAGGAAAAAATACGCATCTCCTTTTCTGTACCAGTGGTATTCTACTTCGCCGTCTGTTGAGTGATCGGAACTTGAGTGGTGTAACACATGTTTTTGTTGATGAAATTCATGAACGAGGCATGAATGAAGACTTCTTGTTGATTGTATTGAAAGATCTGCTTCCACGCCGTCGGGATCTAAGATTGGTTTTGATGAGTGCCACTCTGAATGCGGAGCTATTTTCAAATTTTTTCGGAGGAGCACCAACAATTCACATTCCGGGTTTCACATATCCAGTGAGAGCACACTTTTTGGAAGATGTACTGGAAATGACTGGGTATAAGCTGACTTCTTTCAACCAAATTGATGACTATGGCCAAGACAAACTGTGGAAAACGCAGAAGCAGTTAGCGCCTCGCAAAAGGAAAAACCAGATCACTGCTCTAGTTGAGGATGCTCTTAGTAAATCAAGTTTTGAGAATTATAGCCCCAGAGCACGTGATTCATTATCATGTTGGACGGCTGATTGCATAGGATTTAATCTTATTGAAGCAGTTTTATGTCATATATGTCGGAAGGAACGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGAGGATATCAGCTGTTTGAGGGATCAACTTAAAGCCCATCCTCTTTTAGGTGATCCTAACAGGGTTCTGCTTATAACATGCCATGGGTCAATGACCACGTCTGAACAGAACCTCATTTTCGCGAGACCGCCTCCTAATGTACGTAAAATTGTTCTTGCTACTAATATGGCAGAAGCAAGCATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCACATATGATGCTTTGAATAATACACCTTGTCTACTACCTTCTTGGATATCCCAAGCATCTGCTCGGCAAAGAAGGGGAAGGGCTGGCCGGGTGCAGCCAGGCGAATGTTACCACCTTTACCCCAAATGTGTGTATCATGCGTTTGCAGAATACCAACTTCCTGAACTTCTAAGGACTCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTTTGCAGGTTGGTAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCACCTGAACCCCTTGCTGTTCAAAATGCTGTTGGCTTTCTTAAGTTGATTGGGGCATTGGATGAGAATGAAAATTTGACAAATCTCGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTAAGTGTCCGGGATCCATTCCTTTTGCCTCAAGACAAAAAAGATTTAGCGGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTATAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCAGAAAGAGAAGGATCAGCGTATGAATACTGCTGGAGAAATTTTCTCTCTGCCCAGACACTTCAAGCTATTCATTCTCTTCGGAAGCAGTTTAGCTACATCTTAAGAGACGCTGGCTTGGTGGATGCAGATGCAAGCATTAACAACAAGCTAAGTCACAATCAGTCATTGGTCCGCGCAATCATATGTTCCGGACTATTCCCTGGCATAGCATCTGTAGTGCACCGAGAGACATCCATGTCTTTCAAAACTATGGATGATGGCCAGGTTTTACTATATGCTAATTCTGTAAATGCACGTTACCAAACAATTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAGTAAAGGTCAACACAGTTTTCATACGCGATTCCACTGGTGTCTCTGATTCCATTTTGATCCTTTTTGGTGGTGCCCTAAACCATGGTATCCAGGCTGGGCATTTGAAAATGTTGGACGGCTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGCTTAAAGAAGAGCTTAATGAGCTTATTCAGAAGAAGCTTCAAGATCCAAGCCTCGATATCCACAAGGAAGGAAAGTATCTCATACTTGCTGTTCAAGAGCTGGTATCTGGAGATCAAAGTGAAGGAAGATTTGTGTTTGGCCGTGAAAACAGGAAGCCAAAGGAGTCTGGTGATAACAGCAGATTCACAAGGGATGGAACCAATCCTAAGAGTTTGCTACAAACTCTATTGATGAGAGCAGGACATTCACCTCCAAAATACAAAACCAAGCACCTCAAGACAAATGAGTTCAGAGCACTTGTGGAGTTCAAGGGCATGCAATTTGTTGGGAAGCCCAAGAAAAATAAGCAGCTTGCCGAAAGGGATGCTGCCATAGAAGCCCTGGCCTGGTTGACTCACACCTCAGACAACAGTCGTGATGAAGATGACAACTCCCCACCTGATGTCACTAACAACATGCTGAAACTTCTGGGGAAACGTAGAAGATCAAAGCGCCACTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1027

Amino Acids

115.34

Weight (kDa)

8.81

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 136 - 290 1.9e-09 DEAD/DEAH box helicase
Helicase_C PF00271 397 - 526 3.1e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 590 - 618 2.5e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 619 - 679 5.1e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 746 - 827 4.7e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 836 - 894 2.2e-23 C-terminal domain in RNA helicases
dsrm PF00035 927 - 989 6.7e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1325
AasI GACNNNNNNGTC 1 cut(s) 2302
Acc16I TGCGCA 1 cut(s) 311
Acc36I ACCTGC 1 cut(s) 1690
AccB1I GGYRCC 1 cut(s) 2477
AccI GTMKAC 1 cut(s) 1516
AccII CGCG 3 cut(s) 1373, 2241, 2433
AccIII TCCGGA 2 cut(s) 372, 2255
AciI CCGC 6 cut(s) 67, 181, 866, 1379, 1982, 2239
AcoI YGGCCR 6 cut(s) 517, 1009, 1217, 1570, 2326, 2713
AcsI RAATTY 6 cut(s) 759, 880, 1669, 1718, 1813, 2104
AcuI CTGAAG 2 cut(s) 278, 369
AcvI CACGTG 1 cut(s) 1133
AfaI GTAC 4 cut(s) 547, 685, 957, 1392
AfiI CCNNNNNNNGG 7 cut(s) 76, 1298, 1865, 1866, 2385, 2389, 2961
AflII CTTAAG 2 cut(s) 1781, 2167
AflIII ACRYGT 1 cut(s) 742
AjiI CACGTC 1 cut(s) 1350
AjnI CCWGG 7 cut(s) 1219, 1582, 2266, 2328, 2496, 2955, 2960
Alw21I GWGCWC 5 cut(s) 898, 940, 1132, 2289, 2872
Alw26I GTCTC 6 cut(s) 449, 1369, 1475, 2166, 2288, 2452
Alw44I GTGCAC 1 cut(s) 2285
AlwNI CAGNNNCTG 2 cut(s) 409, 1226
Aor13HI TCCGGA 2 cut(s) 372, 2255
AoxI GGCC 7 cut(s) 517, 1009, 1217, 1570, 2326, 2713, 2958
ApaLI GTGCAC 1 cut(s) 2285
ApeKI GCWGC 5 cut(s) 409, 1579, 1730, 2923, 2942
ApoI RAATTY 6 cut(s) 759, 880, 1669, 1718, 1813, 2104
AspLEI GCGC 5 cut(s) 90, 312, 1048, 2243, 3069
AspS9I GGNCC 1 cut(s) 2236
AsuC2I CCSGG 3 cut(s) 915, 1574, 1943
AsuHPI GGTGA 7 cut(s) 215, 336, 629, 1313, 2411, 2756, 2814
AvaII GGWCC 1 cut(s) 2236
BaeGI GKGCMC 2 cut(s) 2289, 2482
BaeI ACNNNNGTAYC 2 cut(s) 2476, 2509
BalI TGGCCA 2 cut(s) 1011, 2328
BamHI GGATCC 2 cut(s) 1945, 2545
BanI GGYRCC 1 cut(s) 2477
BbrPI CACGTG 1 cut(s) 1133
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 5 cut(s) 898, 940, 1132, 2289, 2872
BbvI GCAGC 5 cut(s) 396, 1591, 1717, 2929, 2935
BccI CCATC 7 cut(s) 263, 394, 1296, 2049, 2318, 2551, 2762
BceAI ACGGC 7 cut(s) 504, 690, 807, 1170, 1232, 2539, 2700
BcgI CGANNNNNNTGC 2 cut(s) 2921, 2955
BciT130I CCWGG 7 cut(s) 1221, 1584, 2268, 2330, 2498, 2957, 2962
BciVI GTATCC 1 cut(s) 2504
BclI TGATCA 1 cut(s) 2023
BcnI CCSGG 3 cut(s) 915, 1574, 1943
BcoDI GTCTC 6 cut(s) 449, 1369, 1475, 2166, 2288, 2452
BfaI CTAG 6 cut(s) 365, 497, 1079, 1865, 1998, 2550
BfmI CTRYAG 3 cut(s) 532, 594, 2280
BfoI RGCGCY 3 cut(s) 91, 1049, 3070
BfrI CTTAAG 2 cut(s) 1781, 2167
BfuAI ACCTGC 1 cut(s) 1690
BfuI GTATCC 1 cut(s) 2504
BglII AGATCT 1 cut(s) 808
BisI GCNGC 5 cut(s) 410, 1580, 1731, 2924, 2943
BlsI GCNGC 5 cut(s) 411, 1581, 1732, 2925, 2944
Bme18I GGWCC 1 cut(s) 2236
BmgBI CACGTC 1 cut(s) 1350
BmgT120I GGNCC 1 cut(s) 2236
BmiI GGNNCC 5 cut(s) 51, 1947, 2479, 2547, 2773
BmrI ACTGGG 2 cut(s) 978, 1254
BmuI ACTGGG 2 cut(s) 978, 1254
BoxI GACNNNNGTC 1 cut(s) 2985
BpiI GAAGAC 1 cut(s) 789
BplI GAGNNNNNCTC 4 cut(s) 841, 873, 1366, 1398
BpmI CTGGAG 2 cut(s) 2119, 2703
BpuEI CTTGAG 3 cut(s) 748, 1947, 2834
BpuMI CCSGG 3 cut(s) 915, 1574, 1943
BsaAI YACGTR 3 cut(s) 317, 1133, 1394
BsaI GGTCTC 2 cut(s) 1369, 1475
BsaJI CCNNGG 6 cut(s) 520, 1333, 2266, 2384, 2488, 2955
BsaWI WCCGGW 2 cut(s) 372, 2255
Bsc4I CCNNNNNNNGG 7 cut(s) 76, 1298, 1865, 1866, 2385, 2389, 2961
Bse1I ACTGG 8 cut(s) 463, 687, 929, 963, 973, 1249, 1850, 2446
Bse3DI GCAATG 1 cut(s) 1897
BseAI TCCGGA 2 cut(s) 372, 2255
BseBI CCWGG 7 cut(s) 1221, 1584, 2268, 2330, 2498, 2957, 2962
BseDI CCNNGG 6 cut(s) 520, 1333, 2266, 2384, 2488, 2955
BseLI CCNNNNNNNGG 7 cut(s) 76, 1298, 1865, 1866, 2385, 2389, 2961
BseMI GCAATG 1 cut(s) 1897
BseMII CTCAG 2 cut(s) 491, 2991
BseNI ACTGG 8 cut(s) 463, 687, 929, 963, 973, 1249, 1850, 2446
BseRI GAGGAG 1 cut(s) 906
BseSI GKGCMC 2 cut(s) 2289, 2482
BseXI GCAGC 5 cut(s) 396, 1591, 1717, 2929, 2935
BseYI CCCAGC 1 cut(s) 2501
BsgI GTGCAG 1 cut(s) 1598
Bsh1236I CGCG 3 cut(s) 1373, 2241, 2433
BshFI GGCC 7 cut(s) 519, 1011, 1219, 1572, 2328, 2715, 2960
BshNI GGYRCC 1 cut(s) 2477
BsiHKAI GWGCWC 5 cut(s) 898, 940, 1132, 2289, 2872
BsiSI CCGG 5 cut(s) 373, 914, 1573, 1942, 2256
BslFI GGGAC 2 cut(s) 18, 1999
BslI CCNNNNNNNGG 7 cut(s) 76, 1298, 1865, 1866, 2385, 2389, 2961
BsmAI GTCTC 6 cut(s) 449, 1369, 1475, 2166, 2288, 2452
BsmBI CGTCTC 1 cut(s) 2166
BsmFI GGGAC 2 cut(s) 18, 1999
BsmI GAATGC 3 cut(s) 256, 527, 868
BsnI GGCC 7 cut(s) 519, 1011, 1219, 1572, 2328, 2715, 2960
Bso31I GGTCTC 2 cut(s) 1369, 1475
Bsp1286I GDGCHC 6 cut(s) 898, 940, 1132, 2289, 2482, 2872
Bsp13I TCCGGA 2 cut(s) 372, 2255
Bsp1407I TGTACA 1 cut(s) 545
Bsp19I CCATGG 2 cut(s) 1333, 2488
Bsp68I TCGCGA 1 cut(s) 1373
BspACI CCGC 6 cut(s) 67, 181, 866, 1379, 1982, 2239
BspANI GGCC 7 cut(s) 519, 1011, 1219, 1572, 2328, 2715, 2960
BspCNI CTCAG 2 cut(s) 490, 2990
BspEI TCCGGA 2 cut(s) 372, 2255
BspFNI CGCG 3 cut(s) 1373, 2241, 2433
BspHI TCATGA 2 cut(s) 763, 1239
BspLI GGNNCC 5 cut(s) 51, 1947, 2479, 2547, 2773
BspMAI CTGCAG 1 cut(s) 598
BspMI ACCTGC 1 cut(s) 1690
BspQI GCTCTTC 1 cut(s) 2577
BspT107I GGYRCC 1 cut(s) 2477
BspTI CTTAAG 2 cut(s) 1781, 2167
BspTNI GGTCTC 2 cut(s) 1369, 1475
BsrDI GCAATG 1 cut(s) 1897
BsrGI TGTACA 1 cut(s) 545
BsrI ACTGG 8 cut(s) 463, 687, 929, 963, 973, 1249, 1850, 2446
BssECI CCNNGG 6 cut(s) 520, 1333, 2266, 2384, 2488, 2955
BssT1I CCWWGG 3 cut(s) 1333, 2384, 2488
Bst2UI CCWGG 7 cut(s) 1221, 1584, 2268, 2330, 2498, 2957, 2962
Bst4CI ACNGT 5 cut(s) 209, 625, 1023, 2422, 2987
Bst6I CTCTTC 3 cut(s) 60, 2148, 2577
BstAFI CTTAAG 2 cut(s) 1781, 2167
BstAUI TGTACA 1 cut(s) 545
BstBAI YACGTR 3 cut(s) 317, 1133, 1394
BstC8I GCNNGC 9 cut(s) 43, 122, 1429, 1570, 1682, 2179, 2200, 2891, 2928
BstDSI CCRYGG 3 cut(s) 520, 1333, 2488
BstENI CCTNNNNNAGG 1 cut(s) 1296
BstFNI CGCG 3 cut(s) 1373, 2241, 2433
BstH2I RGCGCY 3 cut(s) 91, 1049, 3070
BstHHI GCGC 5 cut(s) 90, 312, 1048, 2243, 3069
BstMAI GTCTC 6 cut(s) 449, 1369, 1475, 2166, 2288, 2452
BstMWI GCNNNNNNNGC 4 cut(s) 1045, 1988, 2593, 2951
BstNI CCWGG 7 cut(s) 1221, 1584, 2268, 2330, 2498, 2957, 2962
BstNSI RCATGY 5 cut(s) 325, 746, 1332, 2893, 3034
BstPAI GACNNNNGTC 1 cut(s) 2985
BstSFI CTRYAG 3 cut(s) 532, 594, 2280
BstSLI GKGCMC 2 cut(s) 2289, 2482
BstSNI TACGTA 2 cut(s) 317, 1394
BstUI CGCG 3 cut(s) 1373, 2241, 2433
BstV1I GCAGC 5 cut(s) 396, 1591, 1717, 2929, 2935
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 6 cut(s) 808, 829, 1855, 1945, 2545, 2617
BstYI RGATCY 6 cut(s) 808, 829, 1855, 1945, 2545, 2617
BsuI GTATCC 1 cut(s) 2504
BsuRI GGCC 7 cut(s) 519, 1011, 1219, 1572, 2328, 2715, 2960
BtgI CCRYGG 3 cut(s) 520, 1333, 2488
BtrI CACGTC 1 cut(s) 1350
BtsI GCAGTG 1 cut(s) 1071
BtsIMutI CAGTG 7 cut(s) 12, 214, 630, 694, 936, 1071, 2439
BtuMI TCGCGA 1 cut(s) 1373
BveI ACCTGC 1 cut(s) 1690
Cac8I GCNNGC 9 cut(s) 43, 122, 1429, 1570, 1682, 2179, 2200, 2891, 2928
CaiI CAGNNNCTG 2 cut(s) 409, 1226
CciI TCATGA 2 cut(s) 763, 1239
CfoI GCGC 5 cut(s) 90, 312, 1048, 2243, 3069
Cfr13I GGNCC 1 cut(s) 2236
CseI GACGC 1 cut(s) 2183
Csp6I GTAC 4 cut(s) 546, 684, 956, 1391
CviQI GTAC 4 cut(s) 546, 684, 956, 1391
DrdI GACNNNNNNGTC 1 cut(s) 2302
DseDI GACNNNNNNGTC 1 cut(s) 2302
EaeI YGGCCR 6 cut(s) 517, 1009, 1217, 1570, 2326, 2713
Eam1104I CTCTTC 3 cut(s) 60, 2148, 2577
EarI CTCTTC 3 cut(s) 60, 2148, 2577
Eco105I TACGTA 2 cut(s) 317, 1394
Eco130I CCWWGG 3 cut(s) 1333, 2384, 2488
Eco31I GGTCTC 2 cut(s) 1369, 1475
Eco32I GATATC 4 cut(s) 447, 1258, 1533, 2632
Eco47I GGWCC 1 cut(s) 2236
Eco57I CTGAAG 2 cut(s) 278, 369
Eco72I CACGTG 1 cut(s) 1133
EcoNI CCTNNNNNAGG 1 cut(s) 1296
EcoRI GAATTC 2 cut(s) 1669, 1718
EcoRII CCWGG 7 cut(s) 1219, 1582, 2266, 2328, 2496, 2955, 2960
EcoRV GATATC 4 cut(s) 447, 1258, 1533, 2632
EcoT14I CCWWGG 3 cut(s) 1333, 2384, 2488
ErhI CCWWGG 3 cut(s) 1333, 2384, 2488
Esp3I CGTCTC 1 cut(s) 2166
FalI AAGNNNNNCTT 8 cut(s) 141, 173, 798, 830, 1648, 1680, 2571, 2603
FaqI GGGAC 2 cut(s) 18, 1999
FauI CCCGC 1 cut(s) 1975
FauNDI CATATG 4 cut(s) 1489, 2028, 2047, 2249
FbaI TGATCA 1 cut(s) 2023
FblI GTMKAC 1 cut(s) 1516
Fnu4HI GCNGC 5 cut(s) 410, 1580, 1731, 2924, 2943
Fsp4HI GCNGC 5 cut(s) 410, 1580, 1731, 2924, 2943
FspBI CTAG 6 cut(s) 365, 497, 1079, 1865, 1998, 2550
FspI TGCGCA 1 cut(s) 311
GlaI GCGC 5 cut(s) 89, 311, 1047, 2242, 3068
GluI GCNGC 5 cut(s) 410, 1580, 1731, 2924, 2943
GsaI CCCAGC 1 cut(s) 2505
GsuI CTGGAG 2 cut(s) 2119, 2703
HaeII RGCGCY 3 cut(s) 91, 1049, 3070
HaeIII GGCC 7 cut(s) 519, 1011, 1219, 1572, 2328, 2715, 2960
HapII CCGG 5 cut(s) 373, 914, 1573, 1942, 2256
HgaI GACGC 1 cut(s) 2183
HhaI GCGC 5 cut(s) 90, 312, 1048, 2243, 3069
Hin6I GCGC 5 cut(s) 88, 310, 1046, 2241, 3067
HinP1I GCGC 5 cut(s) 88, 310, 1046, 2241, 3067
HincII GTYRAC 2 cut(s) 2416, 2967
HindII GTYRAC 2 cut(s) 2416, 2967
HindIII AAGCTT 2 cut(s) 2573, 2609
HpaII CCGG 5 cut(s) 373, 914, 1573, 1942, 2256
HphI GGTGA 7 cut(s) 215, 336, 629, 1313, 2411, 2756, 2814
Hpy166II GTNNAC 6 cut(s) 143, 548, 1517, 2287, 2416, 2967
Hpy8I GTNNAC 6 cut(s) 143, 548, 1517, 2287, 2416, 2967
Hpy99I CGWCG 1 cut(s) 828
HpyCH4III ACNGT 5 cut(s) 209, 625, 1023, 2422, 2987
HpyCH4IV ACGT 7 cut(s) 186, 316, 1132, 1349, 1393, 2362, 3052
HpyF10VI GCNNNNNNNGC 4 cut(s) 1045, 1988, 2593, 2951
HpySE526I ACGT 7 cut(s) 186, 316, 1132, 1349, 1393, 2362, 3052
HspAI GCGC 5 cut(s) 88, 310, 1046, 2241, 3067
Kpn2I TCCGGA 2 cut(s) 372, 2255
Ksp22I TGATCA 1 cut(s) 2023
LguI GCTCTTC 1 cut(s) 2577
LmnI GCTCC 3 cut(s) 524, 868, 893
Lsp1109I GCAGC 5 cut(s) 396, 1591, 1717, 2929, 2935
MaeI CTAG 6 cut(s) 365, 497, 1079, 1865, 1998, 2550
MaeII ACGT 7 cut(s) 186, 316, 1132, 1349, 1393, 2362, 3052
MaeIII GTNAC 8 cut(s) 203, 209, 317, 736, 1592, 2219, 2363, 3019
MflI RGATCY 6 cut(s) 808, 829, 1855, 1945, 2545, 2617
MhlI GDGCHC 6 cut(s) 898, 940, 1132, 2289, 2482, 2872
MlsI TGGCCA 2 cut(s) 1011, 2328
MluNI TGGCCA 2 cut(s) 1011, 2328
MlyI GAGTC 4 cut(s) 509, 1654, 2747, 2962
MmeI TCCRAC 3 cut(s) 1130, 1187, 2499
Mox20I TGGCCA 2 cut(s) 1011, 2328
MroI TCCGGA 2 cut(s) 372, 2255
MscI TGGCCA 2 cut(s) 1011, 2328
MseI TTAA 8 cut(s) 1173, 1281, 1782, 2168, 2205, 2577, 2589, 3079
MslI CAYNNNNRTG 1 cut(s) 1144
Msp20I TGGCCA 2 cut(s) 1011, 2328
MspA1I CMGCKG 2 cut(s) 1263, 1730
MspCI CTTAAG 2 cut(s) 1781, 2167
MspI CCGG 5 cut(s) 373, 914, 1573, 1942, 2256
Mva1269I GAATGC 3 cut(s) 256, 527, 868
MvaI CCWGG 7 cut(s) 1221, 1584, 2268, 2330, 2498, 2957, 2962
MvnI CGCG 3 cut(s) 1373, 2241, 2433
MwoI GCNNNNNNNGC 4 cut(s) 1045, 1988, 2593, 2951
NciI CCSGG 3 cut(s) 915, 1574, 1943
NcoI CCATGG 2 cut(s) 1333, 2488
NdeI CATATG 4 cut(s) 1489, 2028, 2047, 2249
NlaIV GGNNCC 5 cut(s) 51, 1947, 2479, 2547, 2773
NmeAIII GCCGAG 1 cut(s) 1528
NmuCI GTSAC 4 cut(s) 203, 209, 2219, 3019
NruI TCGCGA 1 cut(s) 1373
NsbI TGCGCA 1 cut(s) 311
NspI RCATGY 5 cut(s) 325, 746, 1332, 2893, 3034
PaeI GCATGC 1 cut(s) 2893
PagI TCATGA 2 cut(s) 763, 1239
PciI ACATGT 1 cut(s) 742
PciSI GCTCTTC 1 cut(s) 2577
PctI GAATGC 3 cut(s) 256, 527, 868
PfeI GAWTC 9 cut(s) 257, 341, 400, 512, 1136, 2001, 2435, 2453, 2757
PflFI GACNNNGTC 1 cut(s) 1348
PfoI TCCNGGA 1 cut(s) 1941
PkrI GCNGC 5 cut(s) 411, 1581, 1732, 2925, 2944
PleI GAGTC 4 cut(s) 508, 1654, 2746, 2962
PmaCI CACGTG 1 cut(s) 1133
PmlI CACGTG 1 cut(s) 1133
PpsI GAGTC 4 cut(s) 508, 1654, 2746, 2962
Ppu21I YACGTR 3 cut(s) 317, 1133, 1394
PscI ACATGT 1 cut(s) 742
PshAI GACNNNNGTC 1 cut(s) 2985
PsiI TTATAA 1 cut(s) 1325
Psp6I CCWGG 7 cut(s) 1219, 1582, 2266, 2328, 2496, 2955, 2960
PspCI CACGTG 1 cut(s) 1133
PspFI CCCAGC 1 cut(s) 2501
PspGI CCWGG 7 cut(s) 1219, 1582, 2266, 2328, 2496, 2955, 2960
PspN4I GGNNCC 5 cut(s) 51, 1947, 2479, 2547, 2773
PspPI GGNCC 1 cut(s) 2236
PstI CTGCAG 1 cut(s) 598
PstNI CAGNNNCTG 2 cut(s) 409, 1226
PsuI RGATCY 6 cut(s) 808, 829, 1855, 1945, 2545, 2617
PsyI GACNNNGTC 1 cut(s) 1348
PvuII CAGCTG 2 cut(s) 1263, 1730
RruI TCGCGA 1 cut(s) 1373
RsaI GTAC 4 cut(s) 547, 685, 957, 1392
RsaNI GTAC 4 cut(s) 546, 684, 956, 1391
RseI CAYNNNNRTG 1 cut(s) 1144
SapI GCTCTTC 1 cut(s) 2577
SaqAI TTAA 8 cut(s) 1173, 1281, 1782, 2168, 2205, 2577, 2589, 3079
SatI GCNGC 5 cut(s) 410, 1580, 1731, 2924, 2943
Sau96I GGNCC 1 cut(s) 2236
SchI GAGTC 4 cut(s) 509, 1654, 2747, 2962
SduI GDGCHC 6 cut(s) 898, 940, 1132, 2289, 2482, 2872
SfcI CTRYAG 3 cut(s) 532, 594, 2280
SinI GGWCC 1 cut(s) 2236
SmiMI CAYNNNNRTG 1 cut(s) 1144
SmlI CTYRAG 5 cut(s) 727, 1781, 1962, 2167, 2849
SmoI CTYRAG 5 cut(s) 727, 1781, 1962, 2167, 2849
SnaBI TACGTA 2 cut(s) 317, 1394
SphI GCATGC 1 cut(s) 2893
SsiI CCGC 6 cut(s) 67, 181, 866, 1379, 1982, 2239
SspI AATATT 1 cut(s) 1835
SspMI CTAG 6 cut(s) 365, 497, 1079, 1865, 1998, 2550
StyI CCWWGG 3 cut(s) 1333, 2384, 2488
TaaI ACNGT 5 cut(s) 209, 625, 1023, 2422, 2987
TaiI ACGT 7 cut(s) 189, 319, 1135, 1352, 1396, 2365, 3055
TaqI TCGA 4 cut(s) 219, 556, 640, 2628
TatI WGTACW 2 cut(s) 545, 955
TfiI GAWTC 9 cut(s) 257, 341, 400, 512, 1136, 2001, 2435, 2453, 2757
Tru1I TTAA 8 cut(s) 1173, 1281, 1782, 2168, 2205, 2577, 2589, 3079
Tru9I TTAA 8 cut(s) 1173, 1281, 1782, 2168, 2205, 2577, 2589, 3079
TscAI CASTG 7 cut(s) 19, 214, 630, 694, 936, 1078, 2446
TseFI GTSAC 4 cut(s) 203, 209, 2219, 3019
TseI GCWGC 5 cut(s) 409, 1579, 1730, 2923, 2942
Tsp45I GTSAC 4 cut(s) 203, 209, 2219, 3019
TspRI CASTG 7 cut(s) 19, 214, 630, 694, 936, 1078, 2446
Tth111I GACNNNGTC 1 cut(s) 1348
Vha464I CTTAAG 2 cut(s) 1781, 2167
VneI GTGCAC 1 cut(s) 2285
VpaK11BI GGWCC 1 cut(s) 2236
XagI CCTNNNNNAGG 1 cut(s) 1296
XapI RAATTY 6 cut(s) 759, 880, 1669, 1718, 1813, 2104
XbaI TCTAGA 1 cut(s) 1997
XceI RCATGY 5 cut(s) 325, 746, 1332, 2893, 3034
XcmI CCANNNNNNNNNTGG 1 cut(s) 2953
XmiI GTMKAC 1 cut(s) 1516
XspI CTAG 6 cut(s) 365, 497, 1079, 1865, 1998, 2550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.