pycom04g07060

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005499
Physical Location & Seq
Forward (+)
7414363 .. 7426670
12308 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g07060.12

Sequence Viewer

Length: 3471 bp
ATGCGTAATTCGAGAGCAGCACTTGGTCTCCTTCTCAAGTCAGTCTCCAACAATAACCCTTGTTGTTTTCCTTCTTCCCGCAAGACAAACGGTTGCTGCCGCACTTTGCTTCTTCTACTCCAACCCCACTCTGCTTCTTCAATTTCCGACGGCGGAGCCCGATTCCTCATTTCAAAGCGCGCCTTTTGCGGGTACGCCGCCGAGCAGTTCTCCGACGATGAGTACGCGTGCGAATTCGAAGGCCAAAAGGCATCGTCTTCAGTGGCGAACATTGACGAGTGGAAATGGAAAATGAGCTTGCTATTACGCAGCGAAAAGGACCAAGAGATTGTATCCAGAGATAAGAGAGATAGGAGAGACTATGAGCAGATATCTAACCTTGCCAAAAGGATGGGACTTTATTGTGAAATATATGGGAAAGCGGTGGTGGCCAGCAAGGTCCCTCTTCCAAACTACAGGCCTGATTTGGATGATAAGCGGCCACAAAGAGAGGTGGTTATCCCACTTGGCTTGCAAAGGAGGGTAGAGGGTTTGCTGCAAGAGCACCTCGATCGACTCCAGTTAAACTCTGGGAAGTTCACTGGAAATAGAGGGGACTCTGAGCACATTGGTCAGGTCGAAAATGCAAACCTGGATGAGAATGCAGATTCTTTTCTAGATGGGTCTGTAATGGAGAAGGTTCTTCAAAGGAGGAGTTTGCGAATGCGCAATATGCAAAGAGCTTGGCAGGAATCACCCGAAGGCAAGAAGATGCTAGACTTCCGCAAATCACTACCGGCTTTCAAGGAGAATGAAAGGTTGCTGCAAGCAATTGCACAGAATCAGGTAATAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAACTTCCTCAATATATCCTAGAGTCAGAAATAGAATCTGGCCGTGGAGCATTCTGTAGCATCATTTGCACACAGCCTCGAAGAATATCTGCTATGGCTGTTGCAGAAAGAGTGTCCGCAGAGAGGGGAGAGCCTCTTGGTGAAACAGTTGGCTATAAAGTTCGATTGGAGGGAATGAAGGGAAAAAATACCCATCTGCTTTTTTGTACAAGTGGTATTTTACTTCGCCGTCTGTTGAGTGATCGTAACTTGAATGGTATAACCCATGTTTTTGTTGATGAAATTCATGAGCGAGGCATGAATGAAGATTTCTTATTGATTGTGCTGAAGGATCTGCTTCCACGCCGTCGGGATTTGAGATTGGTTTTAATGAGTGCTACTCTGAATGCTGAGCTGTTTTCAAGTTATTTTGGAGGAGCTCCTACAATTCATATTCCGGGCTTTACATATCCAGTAAGAGCACACTTTTTAGAAGATGTACTGGAAATGACTGGGTATAAGTTGACTTCCTTCAACCAAATTGATGATTATGGCCAAGACAAACTGTGGAAAACACAGAAGCAGTTGGTGCCTCGAAAAAGGAAAAACCAGATTACTGCTCTGGTTGAGGATGCTCTTAATAAATCAAGCTTTGAGAGTTACAGTGCCAGGGCACGTGATTCACTATCATGTTGGACACCTGATTGCATTGGATTTAATTTAATTGAGGCAGTTTTATGTCATATATGTCGGAAGGAAAGACAAGGTGCTGTTTTAGTATTCATGACTGGGTGGGAGGATATAAGCTCTTTGAGGGATCAACTTAAAGCACATCCACTTTTAGGTGATCCTAACAGGGTTTTTCTTCTAACATGCCATGGGTCAATGGGAACATCTGAACAGAAACTCATATTTGGAAGACCACCTCCTAACATTCGTAAAATAGTCCTTGCAACAAATATGGCAGAAGCAAGTATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCTCGTATGATGCTTTGAATAATACACCTTGTCTGCTACCTTCTTGGATATCGCAAGCATCTGCTCGGCAAAGAAGGGGTAGGGCTGGCCGGGTGCTGCCAGGCGAATGTTACCACCTTTACCCCAAATGCGTTTATCACGCTTTTGCTGAATATCAACTTCCTGAACTGCTCAGAACTCCTTTGAACTCTCTTTGCTTGCAAATTAAAAGTTTGCAGGTTGCTAGTATAGGGGAATTCTTGTCAGCTGCCTTGCAGCCACCGGAACCCCTAGCTGTCCAAAATGCCATTGGTTTTCTTACTTCAATTGGGGCATTAGATGAGAATGAAAATTTGACAAGTCTCGGAAAATATTTGTCAATACTTCCAGTGGATCCCAAACTGGGGAAAATGCTGATTATGGGTGCTGTGTTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGTCTAAGTGTCAGGGATCCTTTCCTTTTGCCTCAAGACAAAAAAGATTTAGCAGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTACAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCTGAAAGAGAAGGATCAGCATATGAATATTGCTGGAGAAATTTCCTTTCTGCTCAAACACTTCAGGCTATCCATTCTCTTCGGAAGCAGTTTAACTACATTTTAAGAGATGCTGGATTGGTGGATGCGGATGCAAGCATTAATAACAAGCTAAGTCACAACCAGTCCTTGGTTCGTGCAATCATATGTTCTGGACTTTTCCCTGGAATAGCATCTGTGGTGCACCGAGAGACGTCCATGTCATTCAAAACGATGGATGATGGCCAGGTTCTACTATTTGCTAATTCTGTGAATGTGCGTTACCAAACAATTCCTTACCCCTGGTTGGTCTTTGGTGAGAAAGTAAGGGTCAATACTGTATTCATACGTGATTCCACTGGCGTATCTGATTCGATCCTGATCCTTTTTGGTGGTGCGTTGAACCATGGGGTCCAGGCTGGGCATTTGAGAATGTTGGATGGTTATATCGACTTCTTCATGGATCCTAGTTTGGTGGACTGCTATTTGAAGCTTAAGGAAGAACTTAATGAGCTTATTCAAAAGAAGCTTGAAGATCCTAGCCTTGATATCCACAAGGAAGGGAAGTACCTGATGCTTGCTGTTCAAGAGCTGGTTTCCGGAGATCAATGTGAAGGGAGATTTGTATTTGGTCGTGACAGCAGAAAGCCGAAGGAATCTGGTGATAACAGTAGATTCACTAAAGACGGAACCAATCCTAAGAGTTTGCTTCAAACTCTGTTGATGAGAGCAGGACATTCTCCTCCAAAATACAAAACAAAGCACCTTAAGACGAACGAGTTCAGAGCACTGGTGGAGTTCAAGGGCATGCAATTTGTCGGGAAACCTAAGAAAAATAAGCAGCTTGCAGAAAGGGATGCTGCTATAGAAGCACTGGCCTGGTTGACTCACACCTCAGAAAACCATCGTGATGAAGAAGACAACTCCCCCCCTGACATCACTGACAACATGCTCAAACTTCTCGGGAAACGTAGAAGATCAAAACGGCAGTCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1157

Amino Acids

130.02

Weight (kDa)

8.8

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2692
AatII GACGTC 1 cut(s) 2690
Acc16I TGCGCA 1 cut(s) 707
Acc36I ACCTGC 1 cut(s) 2080
AccB1I GGYRCC 1 cut(s) 1435
AccB7I CCANNNNNTGG 1 cut(s) 2623
AccII CGCG 2 cut(s) 180, 227
AccIII TCCGGA 1 cut(s) 3071
AcoI YGGCCR 6 cut(s) 429, 479, 907, 1399, 1960, 2716
AcsI RAATTY 5 cut(s) 233, 1149, 2108, 2203, 2494
AcuI CTGAAG 3 cut(s) 243, 1214, 2501
AcvI CACGTG 1 cut(s) 1523
AcyI GRCGYC 1 cut(s) 2687
AfaI GTAC 5 cut(s) 194, 224, 1075, 1347, 3041
AfiI CCNNNNNNNGG 7 cut(s) 189, 990, 1688, 2255, 2256, 2623, 2779
AflII CTTAAG 2 cut(s) 2966, 3239
AflIII ACRYGT 1 cut(s) 225
AjnI CCWGG 8 cut(s) 630, 1514, 1972, 2656, 2718, 2774, 2886, 3350
Alw21I GWGCWC 6 cut(s) 546, 606, 1288, 1330, 2679, 3262
Alw26I GTCTC 7 cut(s) 32, 49, 351, 839, 1865, 2219, 2678
Alw44I GTGCAC 1 cut(s) 2675
Ama87I CYCGRG 1 cut(s) 3434
Aor13HI TCCGGA 1 cut(s) 3071
AoxI GGCC 9 cut(s) 241, 429, 458, 479, 907, 1399, 1960, 2716, 3348
ApaLI GTGCAC 1 cut(s) 2675
ApoI RAATTY 5 cut(s) 233, 1149, 2108, 2203, 2494
ArsI GACNNNNNNTTYG 1 cut(s) 3446
AseI ATTAAT 1 cut(s) 2595
Asp700I GAANNNNTTC 1 cut(s) 3251
AspLEI GCGC 3 cut(s) 180, 182, 708
AspS9I GGNCC 3 cut(s) 319, 439, 2884
AsuC2I CCSGG 2 cut(s) 1305, 1964
AsuHPI GGTGA 5 cut(s) 726, 1019, 1703, 2801, 3146
AsuII TTCGAA 1 cut(s) 237
AvaI CYCGRG 1 cut(s) 3434
AvaII GGWCC 3 cut(s) 319, 439, 2884
BaeGI GKGCMC 2 cut(s) 1522, 2679
BaeI ACNNNNGTAYC 2 cut(s) 2820, 2853
BalI TGGCCA 3 cut(s) 431, 1401, 2718
BamHI GGATCC 3 cut(s) 2245, 2335, 2935
BanI GGYRCC 1 cut(s) 1435
BanII GRGCYC 2 cut(s) 160, 1288
BbrPI CACGTG 1 cut(s) 1523
BbsI GAAGAC 3 cut(s) 249, 1771, 3396
Bbv12I GWGCWC 6 cut(s) 546, 606, 1288, 1330, 2679, 3262
BccI CCATC 8 cut(s) 385, 653, 1068, 2439, 2701, 2708, 2906, 3384
BceAI ACGGC 4 cut(s) 166, 894, 1080, 1197
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 8 cut(s) 632, 1516, 1974, 2658, 2720, 2776, 2888, 3352
BciVI GTATCC 1 cut(s) 343
BclI TGATCA 1 cut(s) 2413
BcnI CCSGG 2 cut(s) 1305, 1964
BcoDI GTCTC 7 cut(s) 32, 49, 351, 839, 1865, 2219, 2678
BfaI CTAG 8 cut(s) 656, 755, 887, 2097, 2144, 2388, 2940, 3012
BfmI CTRYAG 3 cut(s) 454, 922, 3336
BfrI CTTAAG 2 cut(s) 2966, 3239
BfuAI ACCTGC 1 cut(s) 2080
BfuI GTATCC 1 cut(s) 343
BlpI GCTNAGC 1 cut(s) 1257
Bme18I GGWCC 3 cut(s) 319, 439, 2884
BmeT110I CYCGRG 1 cut(s) 3434
BmgT120I GGNCC 3 cut(s) 319, 439, 2884
BmiI GGNNCC 9 cut(s) 157, 441, 1437, 2139, 2247, 2337, 2885, 2937, 3163
BmrI ACTGGG 3 cut(s) 1368, 1644, 2264
BmuI ACTGGG 3 cut(s) 1368, 1644, 2264
BpiI GAAGAC 3 cut(s) 249, 1771, 3396
BplI GAGNNNNNCTC 4 cut(s) 194, 226, 1231, 1263
BpmI CTGGAG 2 cut(s) 542, 2509
Bpu1102I GCTNAGC 1 cut(s) 1257
Bpu14I TTCGAA 1 cut(s) 237
BpuEI CTTGAG 2 cut(s) 20, 2337
BpuMI CCSGG 2 cut(s) 1305, 1964
BsaAI YACGTR 2 cut(s) 1523, 2822
BsaBI GATNNNNATC 1 cut(s) 2852
BsaHI GRCGYC 1 cut(s) 2687
BsaI GGTCTC 2 cut(s) 32, 1865
BsaJI CCNNGG 7 cut(s) 910, 1515, 1723, 2622, 2656, 2774, 2878
BsaWI WCCGGW 2 cut(s) 2134, 3071
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 7 cut(s) 189, 990, 1688, 2255, 2256, 2623, 2779
Bse118I RCCGGY 1 cut(s) 775
Bse3DI GCAATG 1 cut(s) 2287
Bse8I GATNNNNATC 1 cut(s) 2852
BseAI TCCGGA 1 cut(s) 3071
BseBI CCWGG 8 cut(s) 632, 1516, 1974, 2658, 2720, 2776, 2888, 3352
BseDI CCNNGG 7 cut(s) 910, 1515, 1723, 2622, 2656, 2774, 2878
BseJI GATNNNNATC 1 cut(s) 2852
BseLI CCNNNNNNNGG 7 cut(s) 189, 990, 1688, 2255, 2256, 2623, 2779
BseMI GCAATG 1 cut(s) 2287
BseMII CTCAG 4 cut(s) 591, 1248, 2059, 3381
BsePI GCGCGC 1 cut(s) 178
BseRI GAGGAG 3 cut(s) 706, 1296, 3204
BseSI GKGCMC 2 cut(s) 1522, 2679
BseYI CCCAGC 1 cut(s) 2891
Bsh1236I CGCG 2 cut(s) 180, 227
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 9 cut(s) 243, 431, 460, 481, 909, 1401, 1962, 2718, 3350
BshNI GGYRCC 1 cut(s) 1435
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 6 cut(s) 546, 606, 1288, 1330, 2679, 3262
BsiHKCI CYCGRG 1 cut(s) 3434
BsiSI CCGG 5 cut(s) 776, 1304, 1963, 2135, 3072
BslFI GGGAC 4 cut(s) 408, 425, 608, 2389
BslI CCNNNNNNNGG 7 cut(s) 189, 990, 1688, 2255, 2256, 2623, 2779
BsmAI GTCTC 7 cut(s) 32, 49, 351, 839, 1865, 2219, 2678
BsmBI CGTCTC 1 cut(s) 2678
BsmFI GGGAC 4 cut(s) 408, 425, 608, 2389
BsmI GAATGC 4 cut(s) 646, 708, 917, 1258
BsnI GGCC 9 cut(s) 243, 431, 460, 481, 909, 1401, 1962, 2718, 3350
Bso31I GGTCTC 2 cut(s) 32, 1865
BsoBI CYCGRG 1 cut(s) 3434
Bsp119I TTCGAA 1 cut(s) 237
Bsp1286I GDGCHC 8 cut(s) 160, 546, 606, 1288, 1330, 1522, 2679, 3262
Bsp13I TCCGGA 1 cut(s) 3071
Bsp1407I TGTACA 1 cut(s) 1073
Bsp1720I GCTNAGC 1 cut(s) 1257
Bsp19I CCATGG 2 cut(s) 1723, 2878
BspANI GGCC 9 cut(s) 243, 431, 460, 481, 909, 1401, 1962, 2718, 3350
BspCNI CTCAG 4 cut(s) 592, 1249, 2058, 3380
BspEI TCCGGA 1 cut(s) 3071
BspFNI CGCG 2 cut(s) 180, 227
BspHI TCATGA 2 cut(s) 1153, 1629
BspLI GGNNCC 9 cut(s) 157, 441, 1437, 2139, 2247, 2337, 2885, 2937, 3163
BspMI ACCTGC 1 cut(s) 2080
BspT104I TTCGAA 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 1435
BspTI CTTAAG 2 cut(s) 2966, 3239
BspTNI GGTCTC 2 cut(s) 32, 1865
BsrDI GCAATG 1 cut(s) 2287
BsrFI RCCGGY 1 cut(s) 775
BsrGI TGTACA 1 cut(s) 1073
BssAI RCCGGY 1 cut(s) 775
BssECI CCNNGG 7 cut(s) 910, 1515, 1723, 2622, 2656, 2774, 2878
BssHII GCGCGC 1 cut(s) 178
BssNI GRCGYC 1 cut(s) 2687
BssT1I CCWWGG 3 cut(s) 1723, 2622, 2878
Bst2UI CCWGG 8 cut(s) 632, 1516, 1974, 2658, 2720, 2776, 2888, 3352
Bst4CI ACNGT 7 cut(s) 92, 1015, 1413, 1511, 2411, 2812, 3143
Bst6I CTCTTC 2 cut(s) 450, 2538
BstACI GRCGYC 1 cut(s) 2687
BstAFI CTTAAG 2 cut(s) 2966, 3239
BstAPI GCANNNNNTGC 2 cut(s) 933, 1435
BstAUI TGTACA 1 cut(s) 1073
BstBAI YACGTR 2 cut(s) 1523, 2822
BstBI TTCGAA 1 cut(s) 237
BstDEI CTNAG 8 cut(s) 600, 1257, 2045, 2324, 2606, 3171, 3300, 3367
BstDSI CCRYGG 3 cut(s) 910, 1723, 2878
BstFNI CGCG 2 cut(s) 180, 227
BstHHI GCGC 3 cut(s) 180, 182, 708
BstMAI GTCTC 7 cut(s) 32, 49, 351, 839, 1865, 2219, 2678
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 8 cut(s) 186, 428, 541, 712, 933, 1435, 2378, 3341
BstNI CCWGG 8 cut(s) 632, 1516, 1974, 2658, 2720, 2776, 2888, 3352
BstNSI RCATGY 3 cut(s) 1722, 3283, 3424
BstSFI CTRYAG 3 cut(s) 454, 922, 3336
BstSLI GKGCMC 2 cut(s) 1522, 2679
BstUI CGCG 2 cut(s) 180, 227
BstV2I GAAGAC 3 cut(s) 249, 1771, 3396
BstX2I RGATCY 5 cut(s) 1198, 2245, 2335, 2935, 3007
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 5 cut(s) 1198, 2245, 2335, 2935, 3007
BsuI GTATCC 1 cut(s) 343
BsuRI GGCC 9 cut(s) 243, 431, 460, 481, 909, 1401, 1962, 2718, 3350
BtgI CCRYGG 3 cut(s) 910, 1723, 2878
BtsIMutI CAGTG 9 cut(s) 267, 579, 1516, 2247, 2416, 2829, 3260, 3344, 3411
BveI ACCTGC 1 cut(s) 2080
CciI TCATGA 2 cut(s) 1153, 1629
CfoI GCGC 3 cut(s) 180, 182, 708
Cfr10I RCCGGY 1 cut(s) 775
Cfr13I GGNCC 3 cut(s) 319, 439, 2884
Csp6I GTAC 5 cut(s) 193, 223, 1074, 1346, 3040
CspCI CAANNNNNGTGG 2 cut(s) 492, 527
CviQI GTAC 5 cut(s) 193, 223, 1074, 1346, 3040
DdeI CTNAG 8 cut(s) 600, 1257, 2045, 2324, 2606, 3171, 3300, 3367
DrdI GACNNNNNNGTC 1 cut(s) 2692
DseDI GACNNNNNNGTC 1 cut(s) 2692
EaeI YGGCCR 6 cut(s) 429, 479, 907, 1399, 1960, 2716
Eam1104I CTCTTC 2 cut(s) 450, 2538
EarI CTCTTC 2 cut(s) 450, 2538
EciI GGCGGA 1 cut(s) 168
Ecl136II GAGCTC 1 cut(s) 1286
Eco130I CCWWGG 3 cut(s) 1723, 2622, 2878
Eco147I AGGCCT 1 cut(s) 460
Eco24I GRGCYC 2 cut(s) 160, 1288
Eco31I GGTCTC 2 cut(s) 32, 1865
Eco32I GATATC 4 cut(s) 372, 837, 1923, 3022
Eco47I GGWCC 3 cut(s) 319, 439, 2884
Eco53kI GAGCTC 1 cut(s) 1286
Eco57I CTGAAG 3 cut(s) 243, 1214, 2501
Eco72I CACGTG 1 cut(s) 1523
Eco88I CYCGRG 1 cut(s) 3434
EcoICRI GAGCTC 1 cut(s) 1286
EcoO109I RGGNCCY 1 cut(s) 439
EcoRI GAATTC 2 cut(s) 233, 2108
EcoRII CCWGG 8 cut(s) 630, 1514, 1972, 2656, 2718, 2774, 2886, 3350
EcoRV GATATC 4 cut(s) 372, 837, 1923, 3022
EcoT14I CCWWGG 3 cut(s) 1723, 2622, 2878
EcoT38I GRGCYC 2 cut(s) 160, 1288
ErhI CCWWGG 3 cut(s) 1723, 2622, 2878
Esp3I CGTCTC 1 cut(s) 2678
FalI AAGNNNNNCTT 2 cut(s) 167, 199
FaqI GGGAC 4 cut(s) 408, 425, 608, 2389
FauI CCCGC 2 cut(s) 86, 182
FauNDI CATATG 4 cut(s) 2418, 2437, 2476, 2639
FbaI TGATCA 1 cut(s) 2413
FriOI GRGCYC 2 cut(s) 160, 1288
FspBI CTAG 8 cut(s) 656, 755, 887, 2097, 2144, 2388, 2940, 3012
FspI TGCGCA 1 cut(s) 707
GlaI GCGC 3 cut(s) 179, 181, 707
GsaI CCCAGC 1 cut(s) 2895
GsuI CTGGAG 2 cut(s) 542, 2509
HaeIII GGCC 9 cut(s) 243, 431, 460, 481, 909, 1401, 1962, 2718, 3350
HapII CCGG 5 cut(s) 776, 1304, 1963, 2135, 3072
HhaI GCGC 3 cut(s) 180, 182, 708
Hin1I GRCGYC 1 cut(s) 2687
Hin6I GCGC 3 cut(s) 178, 180, 706
HinP1I GCGC 3 cut(s) 178, 180, 706
HincII GTYRAC 2 cut(s) 1371, 3357
HindII GTYRAC 2 cut(s) 1371, 3357
HindIII AAGCTT 3 cut(s) 1495, 2963, 2999
HpaII CCGG 5 cut(s) 776, 1304, 1963, 2135, 3072
HphI GGTGA 5 cut(s) 726, 1019, 1703, 2801, 3146
Hpy166II GTNNAC 5 cut(s) 579, 1371, 2677, 2950, 3357
Hpy8I GTNNAC 5 cut(s) 579, 1371, 2677, 2950, 3357
Hpy99I CGWCG 3 cut(s) 152, 218, 1218
HpyCH4III ACNGT 7 cut(s) 92, 1015, 1413, 1511, 2411, 2812, 3143
HpyCH4IV ACGT 4 cut(s) 1522, 2687, 2821, 3442
HpyF10VI GCNNNNNNNGC 8 cut(s) 186, 428, 541, 712, 933, 1435, 2378, 3341
HpyF3I CTNAG 8 cut(s) 600, 1257, 2045, 2324, 2606, 3171, 3300, 3367
HpySE526I ACGT 4 cut(s) 1522, 2687, 2821, 3442
Hsp92I GRCGYC 1 cut(s) 2687
HspAI GCGC 3 cut(s) 178, 180, 706
Kpn2I TCCGGA 1 cut(s) 3071
Ksp22I TGATCA 1 cut(s) 2413
LmnI GCTCC 4 cut(s) 155, 914, 1283, 1291
MaeI CTAG 8 cut(s) 656, 755, 887, 2097, 2144, 2388, 2940, 3012
MaeII ACGT 4 cut(s) 1522, 2687, 2821, 3442
MaeIII GTNAC 6 cut(s) 1112, 1505, 1982, 2609, 2753, 3107
MfeI CAATTG 2 cut(s) 810, 2178
MflI RGATCY 5 cut(s) 1198, 2245, 2335, 2935, 3007
MhlI GDGCHC 8 cut(s) 160, 546, 606, 1288, 1330, 1522, 2679, 3262
MlsI TGGCCA 3 cut(s) 431, 1401, 2718
MluI ACGCGT 1 cut(s) 225
MluNI TGGCCA 3 cut(s) 431, 1401, 2718
MlyI GAGTC 4 cut(s) 549, 590, 899, 3352
MmeI TCCRAC 7 cut(s) 72, 145, 171, 237, 1520, 1577, 2889
Mox20I TGGCCA 3 cut(s) 431, 1401, 2718
MroI TCCGGA 1 cut(s) 3071
MroXI GAANNNNTTC 1 cut(s) 3251
MscI TGGCCA 3 cut(s) 431, 1401, 2718
MslI CAYNNNNRTG 2 cut(s) 1534, 3381
Msp20I TGGCCA 3 cut(s) 431, 1401, 2718
MspA1I CMGCKG 1 cut(s) 2120
MspCI CTTAAG 2 cut(s) 2966, 3239
MspI CCGG 5 cut(s) 776, 1304, 1963, 2135, 3072
MunI CAATTG 2 cut(s) 810, 2178
Mva1269I GAATGC 4 cut(s) 646, 708, 917, 1258
MvaI CCWGG 8 cut(s) 632, 1516, 1974, 2658, 2720, 2776, 2888, 3352
MvnI CGCG 2 cut(s) 180, 227
MwoI GCNNNNNNNGC 8 cut(s) 186, 428, 541, 712, 933, 1435, 2378, 3341
NciI CCSGG 2 cut(s) 1305, 1964
NcoI CCATGG 2 cut(s) 1723, 2878
NdeI CATATG 4 cut(s) 2418, 2437, 2476, 2639
NlaIV GGNNCC 9 cut(s) 157, 441, 1437, 2139, 2247, 2337, 2885, 2937, 3163
NmeAIII GCCGAG 2 cut(s) 226, 1918
NmuCI GTSAC 2 cut(s) 2609, 3107
NsbI TGCGCA 1 cut(s) 707
NspI RCATGY 3 cut(s) 1722, 3283, 3424
NspV TTCGAA 1 cut(s) 237
PaeI GCATGC 1 cut(s) 3283
PagI TCATGA 2 cut(s) 1153, 1629
PauI GCGCGC 1 cut(s) 178
PceI AGGCCT 1 cut(s) 460
PctI GAATGC 4 cut(s) 646, 708, 917, 1258
PdmI GAANNNNTTC 1 cut(s) 3251
PflMI CCANNNNNTGG 1 cut(s) 2623
Ple19I CGATCG 1 cut(s) 553
PleI GAGTC 4 cut(s) 549, 590, 898, 3352
PmaCI CACGTG 1 cut(s) 1523
PmlI CACGTG 1 cut(s) 1523
PpsI GAGTC 4 cut(s) 549, 590, 898, 3352
Ppu21I YACGTR 2 cut(s) 1523, 2822
PpuMI RGGWCCY 1 cut(s) 439
PshBI ATTAAT 1 cut(s) 2595
Psp124BI GAGCTC 1 cut(s) 1288
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 8 cut(s) 630, 1514, 1972, 2656, 2718, 2774, 2886, 3350
PspCI CACGTG 1 cut(s) 1523
PspFI CCCAGC 1 cut(s) 2891
PspGI CCWGG 8 cut(s) 630, 1514, 1972, 2656, 2718, 2774, 2886, 3350
PspN4I GGNNCC 9 cut(s) 157, 441, 1437, 2139, 2247, 2337, 2885, 2937, 3163
PspPI GGNCC 3 cut(s) 319, 439, 2884
PspPPI RGGWCCY 1 cut(s) 439
PsuI RGATCY 5 cut(s) 1198, 2245, 2335, 2935, 3007
PteI GCGCGC 1 cut(s) 178
PvuI CGATCG 1 cut(s) 553
PvuII CAGCTG 1 cut(s) 2120
RsaI GTAC 5 cut(s) 194, 224, 1075, 1347, 3041
RsaNI GTAC 5 cut(s) 193, 223, 1074, 1346, 3040
RseI CAYNNNNRTG 2 cut(s) 1534, 3381
SacI GAGCTC 1 cut(s) 1288
Sau96I GGNCC 3 cut(s) 319, 439, 2884
SchI GAGTC 4 cut(s) 549, 590, 899, 3352
SduI GDGCHC 8 cut(s) 160, 546, 606, 1288, 1330, 1522, 2679, 3262
SfcI CTRYAG 3 cut(s) 454, 922, 3336
SfuI TTCGAA 1 cut(s) 237
SinI GGWCC 3 cut(s) 319, 439, 2884
SmiMI CAYNNNNRTG 2 cut(s) 1534, 3381
SmlI CTYRAG 4 cut(s) 35, 2352, 2966, 3239
SmoI CTYRAG 4 cut(s) 35, 2352, 2966, 3239
SphI GCATGC 1 cut(s) 3283
SseBI AGGCCT 1 cut(s) 460
SspI AATATT 2 cut(s) 2225, 2483
SspMI CTAG 8 cut(s) 656, 755, 887, 2097, 2144, 2388, 2940, 3012
SstI GAGCTC 1 cut(s) 1288
StuI AGGCCT 1 cut(s) 460
StyI CCWWGG 3 cut(s) 1723, 2622, 2878
TaaI ACNGT 7 cut(s) 92, 1015, 1413, 1511, 2411, 2812, 3143
TaiI ACGT 4 cut(s) 1525, 2690, 2824, 3445
TatI WGTACW 2 cut(s) 1073, 1345
TauI GCSGC 3 cut(s) 102, 200, 481
TscAI CASTG 9 cut(s) 267, 586, 1516, 2247, 2416, 2836, 3267, 3351, 3418
TseFI GTSAC 2 cut(s) 2609, 3107
Tsp45I GTSAC 2 cut(s) 2609, 3107
TspGWI ACGGA 1 cut(s) 3174
TspRI CASTG 9 cut(s) 267, 586, 1516, 2247, 2416, 2836, 3267, 3351, 3418
Van91I CCANNNNNTGG 1 cut(s) 2623
Vha464I CTTAAG 2 cut(s) 2966, 3239
VneI GTGCAC 1 cut(s) 2675
VpaK11BI GGWCC 3 cut(s) 319, 439, 2884
VspI ATTAAT 1 cut(s) 2595
XapI RAATTY 5 cut(s) 233, 1149, 2108, 2203, 2494
XbaI TCTAGA 2 cut(s) 655, 2387
XceI RCATGY 3 cut(s) 1722, 3283, 3424
XcmI CCANNNNNNNNNTGG 2 cut(s) 566, 2159
XmnI GAANNNNTTC 1 cut(s) 3251
XspI CTAG 8 cut(s) 656, 755, 887, 2097, 2144, 2388, 2940, 3012
ZraI GACGTC 1 cut(s) 2688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.