MD04G1078800.v1.1

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005499
Physical Location & Seq
Forward (+)
10801557 .. 10821285
19729 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1078800.v1.1.491

Sequence Viewer

Length: 3474 bp
ATGCGTAATTCGAGGGCAGCACTTGGTCTCCTTCTCAAGTCAGTCTCCAACAATAACCCTTGTTGTTGTTTTCCTTCGTCCCGGAAGACAAACGGTCGCTGCCGCACTTTGCTTCTTCTACTCCAACCCCACTCTTCTTCTTCAATTTCCGACGGCGGAGCCCGATTCCTCATTTCAAAGCGCGCCTTTTGCGGGTATGCCGCCGAGCAGTTCTCCGACGATGAGTACACGTGCGAATTCGAAGGCCAAAAGGCATCATCTTCGGTGGCGAACATTGACGAGTGGAAATGGAAAATGAGCTTGCTATTACGCAGCGAAAAGGACCAAGAGATTGTATCCAGAGATAAGAGAGATAGGAGGGACTATGAGCAGATATCTAACCTTGCCAAAAGGATGGGACTTTATTGTGAAATATATGGGAAAGCGGTGGTGGCCAGCAAGGTCCCTCTTCCAAACTACAGGCCTGATTTGGATGATAAGCGGCCACAAAGAGAGGTGGTTATCCCTCTTGGCTTGCAAAGGAGGGTAGAGGGTTTGCTGCAAGAGCACCTTGATCGACTCCAGTTAAACTCTGGGAAGTTCACTGGAAATAGAGGGGACTCTGAGCACCTTGGTCAGGTCGAAAATGCAAACCTGGATGAGAATGCAGATTCTCTTCTAGATGGGTCTGTAATGGAGAAGGTTCTTCAAAGGAGGAGTTTGCGAATGCGTAATATGCAAAGAGCTTGGCAGGAATCACCCGAAGGCAAGAAGATGCTAGACTTCCGCAAATCACTACCGGCTTTCAAGGAGAATGAAAGGTTGCTGCAAGCAATTGCACGGAATCAGGTAATAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAACTTCCTCAATATATCCTAGAGTCAGAAATAGAATCTGGCCGTGGAGCATTCTGTAGCATCATTTGCACACAGCCTCGAAGAATATCTGCTATGGCTGTTGCAGAAAGAGTGTCGGCAGAGAGGGGAGAGCCTCTTGGTGAAACAGTTGGCTATAAAGTTCGATTGGAGGGAATGAAGGGAAAAAATACCCATCTGCTTTTTTGTACAAGTGGTATTTTACTTCGCCGTCTGTTGAGTGATCGTAACTTGAATGGTATAACCCATGTTTTTGTTGATGAAATTCATGAGCGAGGCATGAATGAAGATTTCTTATTGATTGTGCTGAAGGATCTGCTTCCACGCCGTCGGGATTTGAGATTGGTTTTAATGAGTGCTACTCTGAATGCTGAGCTGTTTTCAAGTTATTTTGGAGGAGCTCCTACAATTCATATTCCGGGCTTTACATATCCAGTAAGAGCACACTTTTTAGAAGATGTACTGGAAATGACTGGATATAAGTTGACTTCCTTCAACCAAATTGATGATTATGGCCAAGACAAACTGTGGAAAACACAGAAGCAGTTAGTGCCTCGGAAAAGGAAAAACCAGATTACTGCTCTGGTTGAGGATGCTCTTAATAAATCAAGCTTTGAGAGTTACAGTGGCAGGGCACGTGATTCACTATCATGTTGGACACCTGATTGCATTGGATTTAATTTAATCGAGGCAGTTTTATGCCATATAAGTCGGAAGGAACGACAAGGTGCTGTTTTAGTATTCATGACTGGGTGGGAGGATATAAGCTCTTTGAGGGATCAACTTAAAGCACATCCACTTTTAGGTGATCCTAACAGGGTTTTGCTTCTAACATGCCATGGGTCAATGGGAACATCTGAACAGAAACTCATATTTGGAAGACCACCTCCTAACATTCGTAAAATAGTCCTTGCAACAAATATGGCAGAAGCAAGTATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCTCGTATGATGCTTTGAATAATACACCTTGTCTGCTACCTTCTTGGATATCGCAAGCATCTGCTCGGCAAAGAAGGGGTAGGGCTGGCCGGGTGCTGCCAGGCGAATGTTACCACCTTTATCCCAAATGTGTTTATCACGCTTTTGCTGAATATCAACTTCCTGAACTGCTCAGAACTCCTTTGAACTCTCTTTGCTTGCAAATTAAAAGTTTGCAGGTTGCTAGTATAGGGGAATTCTTGTCAGCTGCCTTGCAGCCACCGGAACCCCTAGCTGTCCAAAATGCCATTGGTTTTCTTACTTCAATTGGGGCATTAGATGAGAATGAAAATTTGACAAGTCTGGGAAAATATTTGTCAATACTTCCAGTGGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTGTTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGTCTAAGTGTCAGGGATCCTTTCCTTTTGCCTCAAGACAAAAAAGATTTAGCAGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTACAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCTGAAAGAGAAGGATCAGCATATGAATATTGCTGGAGAAATTTCCTTTCTGCTCAAACACTTCAGGCTATCCATTCTCTTCGGAAGCAGTTTAACTACATTTTAAGAGATGCTGGATTGGTGGATGCGGATGCAAGCATTAACAACAAGCTAAGTCACAACCAGTCCTTGGTTCGTGCAATCATATGTTCTGGACTTTTCCCTGGAATAGCATCTGTGGTGCACCGAGAGACGTCCATGTCATTCAAAACGATGGATGATGGCCAGGTTCTACTATTTGCTAATTCTGTGAATGTGCGTTACCAAACAATTCCTTACCCCTGGTTGGTCTTTGGTGAGAAAGTAAGGGTCAATACTGTATTCATACGTGATTCCACTGGCGTATCTGATTCGATCCTGATCCTTTTTGGTGGTGCGTTGAACCATGGGGTCCAGGCTGGGCATTTGAGAATGTTGGACGGTTATATCGACTTCTTCATGGATCCTAGTTTGGTGGACTGCTATTTGAAGCTTAAGGAAGAACTTAATGAGCTTATTCAAAAGAAGCTTGAAGATCCTAGCCTTGATATCCACAAGGAAGGGAAGTACCTTATGCTTGCTGTTCAAGAGCTGGTTTCCGGAGATCAATGTGAAGGGAGATTTGTATTTGGTCGTGACAGCAGAAAGCCGAAGGAATCTGGTGATAACAGTAGATTCACTAAAGACGGAACCAATCCTAAGAGTTTGCTTCAAACTCTGTTGATGAGAGCAGGACATTCTCCTCCAAAGTACAAAACAAAGCACCTTAAGACGAATGAGTTCAGAGCACTGGTGGAGTTCAAGGGCATGCAATTTGTCGGGAAACCTAAGAAAAATAAGCAGCTTGCAGAAAGGGATGCTGCTATAGAAGCACTGGCCTGGTTGACTCATACCTCAGAAAACCATCGCGATGAAGAAGACAACTCCCCCCCTGACGTCACTGACAATATGCTCAAACTTCTCGGGAAACGTAGAAGATCAAAACGGCAGTCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1158

Amino Acids

130.13

Weight (kDa)

8.88

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 268 - 421 1.9e-08 DEAD/DEAH box helicase
Helicase_C PF00271 528 - 657 7.9e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 721 - 749 3.1e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 750 - 810 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 877 - 958 1.9e-16 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 967 - 1025 9.7e-23 C-terminal domain in RNA helicases
dsrm PF00035 1058 - 1120 7.8e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2695
AatII GACGTC 2 cut(s) 2693, 3414
Acc36I ACCTGC 1 cut(s) 2083
AccB7I CCANNNNNTGG 1 cut(s) 2626
AccII CGCG 2 cut(s) 183, 3384
AccIII TCCGGA 1 cut(s) 3074
AciI CCGC 8 cut(s) 103, 156, 192, 201, 425, 481, 766, 2585
AcoI YGGCCR 6 cut(s) 432, 482, 910, 1402, 1963, 2719
AcsI RAATTY 5 cut(s) 236, 1152, 2111, 2206, 2497
AcuI CTGAAG 2 cut(s) 1217, 2504
AcvI CACGTG 2 cut(s) 231, 1526
AcyI GRCGYC 2 cut(s) 2690, 3411
AfaI GTAC 5 cut(s) 227, 1078, 1350, 3044, 3227
AfiI CCNNNNNNNGG 7 cut(s) 192, 616, 1691, 2258, 2259, 2626, 2782
AflII CTTAAG 2 cut(s) 2969, 3242
AflIII ACRYGT 1 cut(s) 228
AhdI GACNNNNNGTC 1 cut(s) 93
AjnI CCWGG 7 cut(s) 633, 1975, 2659, 2721, 2777, 2889, 3353
Alw21I GWGCWC 6 cut(s) 549, 609, 1291, 1333, 2682, 3265
Alw26I GTCTC 5 cut(s) 32, 49, 842, 1868, 2681
Alw44I GTGCAC 1 cut(s) 2678
Ama87I CYCGRG 1 cut(s) 3437
Aor13HI TCCGGA 1 cut(s) 3074
AoxI GGCC 9 cut(s) 244, 432, 461, 482, 910, 1402, 1963, 2719, 3351
ApaLI GTGCAC 1 cut(s) 2678
ApoI RAATTY 5 cut(s) 236, 1152, 2111, 2206, 2497
ArsI GACNNNNNNTTYG 1 cut(s) 3449
Asp700I GAANNNNTTC 1 cut(s) 3254
AspLEI GCGC 2 cut(s) 183, 185
AspS9I GGNCC 3 cut(s) 322, 442, 2887
AsuC2I CCSGG 3 cut(s) 82, 1308, 1967
AsuHPI GGTGA 5 cut(s) 729, 1022, 1706, 2804, 3149
AsuII TTCGAA 1 cut(s) 240
AvaI CYCGRG 1 cut(s) 3437
AvaII GGWCC 3 cut(s) 322, 442, 2887
BaeGI GKGCMC 2 cut(s) 1525, 2682
BaeI ACNNNNGTAYC 2 cut(s) 2823, 2856
BalI TGGCCA 3 cut(s) 434, 1404, 2721
BamHI GGATCC 3 cut(s) 2248, 2338, 2938
BanII GRGCYC 2 cut(s) 163, 1291
BbrPI CACGTG 2 cut(s) 231, 1526
BbsI GAAGAC 3 cut(s) 92, 1774, 3399
Bbv12I GWGCWC 6 cut(s) 549, 609, 1291, 1333, 2682, 3265
BccI CCATC 7 cut(s) 388, 656, 1071, 2442, 2704, 2711, 3387
BceAI ACGGC 4 cut(s) 169, 897, 1083, 1200
BcgI CGANNNNNNTGC 4 cut(s) 243, 277, 536, 570
BciT130I CCWGG 7 cut(s) 635, 1977, 2661, 2723, 2779, 2891, 3355
BciVI GTATCC 1 cut(s) 346
BclI TGATCA 1 cut(s) 2416
BcnI CCSGG 3 cut(s) 82, 1308, 1967
BcoDI GTCTC 5 cut(s) 32, 49, 842, 1868, 2681
BfaI CTAG 9 cut(s) 659, 758, 890, 2100, 2147, 2258, 2391, 2943, 3015
BfmI CTRYAG 3 cut(s) 457, 925, 3339
BfrI CTTAAG 2 cut(s) 2969, 3242
BfuAI ACCTGC 1 cut(s) 2083
BfuI GTATCC 1 cut(s) 346
BlpI GCTNAGC 1 cut(s) 1260
Bme18I GGWCC 3 cut(s) 322, 442, 2887
BmeRI GACNNNNNGTC 1 cut(s) 93
BmeT110I CYCGRG 1 cut(s) 3437
BmgT120I GGNCC 3 cut(s) 322, 442, 2887
BmiI GGNNCC 8 cut(s) 160, 444, 2142, 2250, 2340, 2888, 2940, 3166
BmrI ACTGGG 1 cut(s) 1647
BmuI ACTGGG 1 cut(s) 1647
BpiI GAAGAC 3 cut(s) 92, 1774, 3399
BplI GAGNNNNNCTC 4 cut(s) 197, 229, 1234, 1266
BpmI CTGGAG 2 cut(s) 545, 2512
Bpu1102I GCTNAGC 1 cut(s) 1260
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 2 cut(s) 20, 2340
BpuMI CCSGG 3 cut(s) 82, 1308, 1967
BsaAI YACGTR 3 cut(s) 231, 1526, 2825
BsaBI GATNNNNATC 1 cut(s) 2855
BsaHI GRCGYC 2 cut(s) 2690, 3411
BsaI GGTCTC 2 cut(s) 32, 1868
BsaJI CCNNGG 8 cut(s) 610, 913, 1442, 1726, 2625, 2659, 2777, 2881
BsaWI WCCGGW 2 cut(s) 2137, 3074
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 7 cut(s) 192, 616, 1691, 2258, 2259, 2626, 2782
Bse118I RCCGGY 1 cut(s) 778
Bse3DI GCAATG 1 cut(s) 2290
Bse8I GATNNNNATC 1 cut(s) 2855
BseAI TCCGGA 1 cut(s) 3074
BseBI CCWGG 7 cut(s) 635, 1977, 2661, 2723, 2779, 2891, 3355
BseDI CCNNGG 8 cut(s) 610, 913, 1442, 1726, 2625, 2659, 2777, 2881
BseJI GATNNNNATC 1 cut(s) 2855
BseLI CCNNNNNNNGG 7 cut(s) 192, 616, 1691, 2258, 2259, 2626, 2782
BseMI GCAATG 1 cut(s) 2290
BseMII CTCAG 4 cut(s) 594, 1251, 2062, 3384
BsePI GCGCGC 1 cut(s) 181
BseRI GAGGAG 3 cut(s) 709, 1299, 3207
BseSI GKGCMC 2 cut(s) 1525, 2682
BseYI CCCAGC 1 cut(s) 2894
Bsh1236I CGCG 2 cut(s) 183, 3384
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 9 cut(s) 246, 434, 463, 484, 912, 1404, 1965, 2721, 3353
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 6 cut(s) 549, 609, 1291, 1333, 2682, 3265
BsiHKCI CYCGRG 1 cut(s) 3437
BsiSI CCGG 6 cut(s) 82, 779, 1307, 1966, 2138, 3075
BslFI GGGAC 6 cut(s) 64, 374, 411, 428, 611, 2392
BslI CCNNNNNNNGG 7 cut(s) 192, 616, 1691, 2258, 2259, 2626, 2782
BsmAI GTCTC 5 cut(s) 32, 49, 842, 1868, 2681
BsmBI CGTCTC 1 cut(s) 2681
BsmFI GGGAC 6 cut(s) 64, 374, 411, 428, 611, 2392
BsmI GAATGC 4 cut(s) 649, 711, 920, 1261
BsnI GGCC 9 cut(s) 246, 434, 463, 484, 912, 1404, 1965, 2721, 3353
Bso31I GGTCTC 2 cut(s) 32, 1868
BsoBI CYCGRG 1 cut(s) 3437
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 8 cut(s) 163, 549, 609, 1291, 1333, 1525, 2682, 3265
Bsp13I TCCGGA 1 cut(s) 3074
Bsp1407I TGTACA 1 cut(s) 1076
Bsp1720I GCTNAGC 1 cut(s) 1260
Bsp19I CCATGG 2 cut(s) 1726, 2881
Bsp68I TCGCGA 1 cut(s) 3384
BspACI CCGC 8 cut(s) 103, 156, 192, 201, 425, 481, 766, 2585
BspANI GGCC 9 cut(s) 246, 434, 463, 484, 912, 1404, 1965, 2721, 3353
BspCNI CTCAG 4 cut(s) 595, 1252, 2061, 3383
BspEI TCCGGA 1 cut(s) 3074
BspFNI CGCG 2 cut(s) 183, 3384
BspHI TCATGA 2 cut(s) 1156, 1632
BspLI GGNNCC 8 cut(s) 160, 444, 2142, 2250, 2340, 2888, 2940, 3166
BspMI ACCTGC 1 cut(s) 2083
BspT104I TTCGAA 1 cut(s) 240
BspTI CTTAAG 2 cut(s) 2969, 3242
BspTNI GGTCTC 2 cut(s) 32, 1868
BsrDI GCAATG 1 cut(s) 2290
BsrFI RCCGGY 1 cut(s) 778
BsrGI TGTACA 1 cut(s) 1076
BssAI RCCGGY 1 cut(s) 778
BssECI CCNNGG 8 cut(s) 610, 913, 1442, 1726, 2625, 2659, 2777, 2881
BssHII GCGCGC 1 cut(s) 181
BssNI GRCGYC 2 cut(s) 2690, 3411
BssT1I CCWWGG 4 cut(s) 610, 1726, 2625, 2881
Bst2UI CCWGG 7 cut(s) 635, 1977, 2661, 2723, 2779, 2891, 3355
Bst4CI ACNGT 8 cut(s) 95, 1018, 1416, 1514, 2414, 2815, 2918, 3146
Bst6I CTCTTC 4 cut(s) 139, 453, 660, 2541
BstACI GRCGYC 2 cut(s) 2690, 3411
BstAFI CTTAAG 2 cut(s) 2969, 3242
BstAPI GCANNNNNTGC 2 cut(s) 936, 1438
BstAUI TGTACA 1 cut(s) 1076
BstBAI YACGTR 3 cut(s) 231, 1526, 2825
BstBI TTCGAA 1 cut(s) 240
BstDEI CTNAG 8 cut(s) 603, 1260, 2048, 2327, 2609, 3174, 3303, 3370
BstDSI CCRYGG 3 cut(s) 913, 1726, 2881
BstENI CCTNNNNNAGG 1 cut(s) 614
BstFNI CGCG 2 cut(s) 183, 3384
BstHHI GCGC 2 cut(s) 183, 185
BstMAI GTCTC 5 cut(s) 32, 49, 842, 1868, 2681
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 8 cut(s) 189, 431, 544, 715, 936, 1438, 2381, 3344
BstNI CCWGG 7 cut(s) 635, 1977, 2661, 2723, 2779, 2891, 3355
BstNSI RCATGY 2 cut(s) 1725, 3286
BstSFI CTRYAG 3 cut(s) 457, 925, 3339
BstSLI GKGCMC 2 cut(s) 1525, 2682
BstUI CGCG 2 cut(s) 183, 3384
BstV2I GAAGAC 3 cut(s) 92, 1774, 3399
BstX2I RGATCY 5 cut(s) 1201, 2248, 2338, 2938, 3010
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 5 cut(s) 1201, 2248, 2338, 2938, 3010
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 9 cut(s) 246, 434, 463, 484, 912, 1404, 1965, 2721, 3353
BtgI CCRYGG 3 cut(s) 913, 1726, 2881
BtgZI GCGATG 2 cut(s) 3365, 3399
BtsIMutI CAGTG 8 cut(s) 582, 1519, 2250, 2419, 2832, 3263, 3347, 3414
BtuMI TCGCGA 1 cut(s) 3384
BveI ACCTGC 1 cut(s) 2083
CciI TCATGA 2 cut(s) 1156, 1632
CfoI GCGC 2 cut(s) 183, 185
Cfr10I RCCGGY 1 cut(s) 778
Cfr13I GGNCC 3 cut(s) 322, 442, 2887
Csp6I GTAC 5 cut(s) 226, 1077, 1349, 3043, 3226
CviQI GTAC 5 cut(s) 226, 1077, 1349, 3043, 3226
DdeI CTNAG 8 cut(s) 603, 1260, 2048, 2327, 2609, 3174, 3303, 3370
DrdI GACNNNNNNGTC 1 cut(s) 2695
DriI GACNNNNNGTC 1 cut(s) 93
DseDI GACNNNNNNGTC 1 cut(s) 2695
EaeI YGGCCR 6 cut(s) 432, 482, 910, 1402, 1963, 2719
Eam1104I CTCTTC 4 cut(s) 139, 453, 660, 2541
Eam1105I GACNNNNNGTC 1 cut(s) 93
EarI CTCTTC 4 cut(s) 139, 453, 660, 2541
EciI GGCGGA 1 cut(s) 171
Ecl136II GAGCTC 1 cut(s) 1289
Eco130I CCWWGG 4 cut(s) 610, 1726, 2625, 2881
Eco147I AGGCCT 1 cut(s) 463
Eco24I GRGCYC 2 cut(s) 163, 1291
Eco31I GGTCTC 2 cut(s) 32, 1868
Eco32I GATATC 4 cut(s) 375, 840, 1926, 3025
Eco47I GGWCC 3 cut(s) 322, 442, 2887
Eco53kI GAGCTC 1 cut(s) 1289
Eco57I CTGAAG 2 cut(s) 1217, 2504
Eco72I CACGTG 2 cut(s) 231, 1526
Eco88I CYCGRG 1 cut(s) 3437
EcoICRI GAGCTC 1 cut(s) 1289
EcoNI CCTNNNNNAGG 1 cut(s) 614
EcoO109I RGGNCCY 1 cut(s) 442
EcoRI GAATTC 2 cut(s) 236, 2111
EcoRII CCWGG 7 cut(s) 633, 1975, 2659, 2721, 2777, 2889, 3353
EcoRV GATATC 4 cut(s) 375, 840, 1926, 3025
EcoT14I CCWWGG 4 cut(s) 610, 1726, 2625, 2881
EcoT38I GRGCYC 2 cut(s) 163, 1291
ErhI CCWWGG 4 cut(s) 610, 1726, 2625, 2881
Esp3I CGTCTC 1 cut(s) 2681
FalI AAGNNNNNCTT 4 cut(s) 170, 202, 534, 566
FaqI GGGAC 6 cut(s) 64, 374, 411, 428, 611, 2392
FauI CCCGC 1 cut(s) 185
FauNDI CATATG 4 cut(s) 2421, 2440, 2479, 2642
FbaI TGATCA 1 cut(s) 2416
FriOI GRGCYC 2 cut(s) 163, 1291
FspBI CTAG 9 cut(s) 659, 758, 890, 2100, 2147, 2258, 2391, 2943, 3015
GlaI GCGC 2 cut(s) 182, 184
GsaI CCCAGC 1 cut(s) 2898
GsuI CTGGAG 2 cut(s) 545, 2512
HaeIII GGCC 9 cut(s) 246, 434, 463, 484, 912, 1404, 1965, 2721, 3353
HapII CCGG 6 cut(s) 82, 779, 1307, 1966, 2138, 3075
HhaI GCGC 2 cut(s) 183, 185
Hin1I GRCGYC 2 cut(s) 2690, 3411
Hin6I GCGC 2 cut(s) 181, 183
HinP1I GCGC 2 cut(s) 181, 183
HincII GTYRAC 2 cut(s) 1374, 3360
HindII GTYRAC 2 cut(s) 1374, 3360
HindIII AAGCTT 3 cut(s) 1498, 2966, 3002
HpaII CCGG 6 cut(s) 82, 779, 1307, 1966, 2138, 3075
HphI GGTGA 5 cut(s) 729, 1022, 1706, 2804, 3149
Hpy166II GTNNAC 6 cut(s) 228, 582, 1374, 2680, 2953, 3360
Hpy8I GTNNAC 6 cut(s) 228, 582, 1374, 2680, 2953, 3360
Hpy99I CGWCG 3 cut(s) 155, 221, 1221
HpyCH4III ACNGT 8 cut(s) 95, 1018, 1416, 1514, 2414, 2815, 2918, 3146
HpyCH4IV ACGT 6 cut(s) 230, 1525, 2690, 2824, 3411, 3445
HpyF10VI GCNNNNNNNGC 8 cut(s) 189, 431, 544, 715, 936, 1438, 2381, 3344
HpyF3I CTNAG 8 cut(s) 603, 1260, 2048, 2327, 2609, 3174, 3303, 3370
HpySE526I ACGT 6 cut(s) 230, 1525, 2690, 2824, 3411, 3445
Hsp92I GRCGYC 2 cut(s) 2690, 3411
HspAI GCGC 2 cut(s) 181, 183
Kpn2I TCCGGA 1 cut(s) 3074
Ksp22I TGATCA 1 cut(s) 2416
LmnI GCTCC 4 cut(s) 158, 917, 1286, 1294
MaeI CTAG 9 cut(s) 659, 758, 890, 2100, 2147, 2258, 2391, 2943, 3015
MaeII ACGT 6 cut(s) 230, 1525, 2690, 2824, 3411, 3445
MaeIII GTNAC 7 cut(s) 1115, 1508, 1985, 2612, 2756, 3110, 3412
MfeI CAATTG 2 cut(s) 813, 2181
MflI RGATCY 5 cut(s) 1201, 2248, 2338, 2938, 3010
MhlI GDGCHC 8 cut(s) 163, 549, 609, 1291, 1333, 1525, 2682, 3265
MlsI TGGCCA 3 cut(s) 434, 1404, 2721
MluNI TGGCCA 3 cut(s) 434, 1404, 2721
MlyI GAGTC 4 cut(s) 552, 593, 902, 3355
MmeI TCCRAC 7 cut(s) 72, 148, 174, 240, 1523, 1580, 2892
Mox20I TGGCCA 3 cut(s) 434, 1404, 2721
MroI TCCGGA 1 cut(s) 3074
MroXI GAANNNNTTC 1 cut(s) 3254
MscI TGGCCA 3 cut(s) 434, 1404, 2721
MslI CAYNNNNRTG 2 cut(s) 1537, 3384
Msp20I TGGCCA 3 cut(s) 434, 1404, 2721
MspA1I CMGCKG 1 cut(s) 2123
MspCI CTTAAG 2 cut(s) 2969, 3242
MspI CCGG 6 cut(s) 82, 779, 1307, 1966, 2138, 3075
MunI CAATTG 2 cut(s) 813, 2181
Mva1269I GAATGC 4 cut(s) 649, 711, 920, 1261
MvaI CCWGG 7 cut(s) 635, 1977, 2661, 2723, 2779, 2891, 3355
MvnI CGCG 2 cut(s) 183, 3384
MwoI GCNNNNNNNGC 8 cut(s) 189, 431, 544, 715, 936, 1438, 2381, 3344
NciI CCSGG 3 cut(s) 82, 1308, 1967
NcoI CCATGG 2 cut(s) 1726, 2881
NdeI CATATG 4 cut(s) 2421, 2440, 2479, 2642
NlaIV GGNNCC 8 cut(s) 160, 444, 2142, 2250, 2340, 2888, 2940, 3166
NmeAIII GCCGAG 2 cut(s) 229, 1921
NmuCI GTSAC 3 cut(s) 2612, 3110, 3412
NruI TCGCGA 1 cut(s) 3384
NspI RCATGY 2 cut(s) 1725, 3286
NspV TTCGAA 1 cut(s) 240
PaeI GCATGC 1 cut(s) 3286
PagI TCATGA 2 cut(s) 1156, 1632
PauI GCGCGC 1 cut(s) 181
PceI AGGCCT 1 cut(s) 463
PcsI WCGNNNNNNNCGW 2 cut(s) 1606, 2922
PctI GAATGC 4 cut(s) 649, 711, 920, 1261
PdmI GAANNNNTTC 1 cut(s) 3254
PflMI CCANNNNNTGG 1 cut(s) 2626
PfoI TCCNGGA 1 cut(s) 80
PleI GAGTC 4 cut(s) 552, 593, 901, 3355
PmaCI CACGTG 2 cut(s) 231, 1526
PmlI CACGTG 2 cut(s) 231, 1526
PpsI GAGTC 4 cut(s) 552, 593, 901, 3355
Ppu21I YACGTR 3 cut(s) 231, 1526, 2825
PpuMI RGGWCCY 1 cut(s) 442
Psp124BI GAGCTC 1 cut(s) 1291
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 7 cut(s) 633, 1975, 2659, 2721, 2777, 2889, 3353
PspCI CACGTG 2 cut(s) 231, 1526
PspFI CCCAGC 1 cut(s) 2894
PspGI CCWGG 7 cut(s) 633, 1975, 2659, 2721, 2777, 2889, 3353
PspN4I GGNNCC 8 cut(s) 160, 444, 2142, 2250, 2340, 2888, 2940, 3166
PspPI GGNCC 3 cut(s) 322, 442, 2887
PspPPI RGGWCCY 1 cut(s) 442
PsuI RGATCY 5 cut(s) 1201, 2248, 2338, 2938, 3010
PteI GCGCGC 1 cut(s) 181
PvuII CAGCTG 1 cut(s) 2123
RruI TCGCGA 1 cut(s) 3384
RsaI GTAC 5 cut(s) 227, 1078, 1350, 3044, 3227
RsaNI GTAC 5 cut(s) 226, 1077, 1349, 3043, 3226
RseI CAYNNNNRTG 2 cut(s) 1537, 3384
SacI GAGCTC 1 cut(s) 1291
Sau96I GGNCC 3 cut(s) 322, 442, 2887
SchI GAGTC 4 cut(s) 552, 593, 902, 3355
SduI GDGCHC 8 cut(s) 163, 549, 609, 1291, 1333, 1525, 2682, 3265
SfcI CTRYAG 3 cut(s) 457, 925, 3339
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 3 cut(s) 322, 442, 2887
SmiMI CAYNNNNRTG 2 cut(s) 1537, 3384
SmlI CTYRAG 4 cut(s) 35, 2355, 2969, 3242
SmoI CTYRAG 4 cut(s) 35, 2355, 2969, 3242
SphI GCATGC 1 cut(s) 3286
SseBI AGGCCT 1 cut(s) 463
SsiI CCGC 8 cut(s) 103, 156, 192, 201, 425, 481, 766, 2585
SspI AATATT 2 cut(s) 2228, 2486
SspMI CTAG 9 cut(s) 659, 758, 890, 2100, 2147, 2258, 2391, 2943, 3015
SstI GAGCTC 1 cut(s) 1291
StuI AGGCCT 1 cut(s) 463
StyI CCWWGG 4 cut(s) 610, 1726, 2625, 2881
TaaI ACNGT 8 cut(s) 95, 1018, 1416, 1514, 2414, 2815, 2918, 3146
TaiI ACGT 6 cut(s) 233, 1528, 2693, 2827, 3414, 3448
TaqI TCGA 9 cut(s) 11, 240, 556, 621, 949, 1033, 1575, 2849, 2925
TatI WGTACW 4 cut(s) 225, 1076, 1348, 3225
TauI GCSGC 3 cut(s) 105, 203, 484
TscAI CASTG 8 cut(s) 589, 1519, 2250, 2419, 2839, 3270, 3354, 3421
TseFI GTSAC 3 cut(s) 2612, 3110, 3412
Tsp45I GTSAC 3 cut(s) 2612, 3110, 3412
TspGWI ACGGA 2 cut(s) 835, 3177
TspRI CASTG 8 cut(s) 589, 1519, 2250, 2419, 2839, 3270, 3354, 3421
Van91I CCANNNNNTGG 1 cut(s) 2626
Vha464I CTTAAG 2 cut(s) 2969, 3242
VneI GTGCAC 1 cut(s) 2678
VpaK11BI GGWCC 3 cut(s) 322, 442, 2887
XagI CCTNNNNNAGG 1 cut(s) 614
XapI RAATTY 5 cut(s) 236, 1152, 2111, 2206, 2497
XbaI TCTAGA 2 cut(s) 658, 2390
XceI RCATGY 2 cut(s) 1725, 3286
XcmI CCANNNNNNNNNTGG 2 cut(s) 569, 2162
XmnI GAANNNNTTC 1 cut(s) 3254
XspI CTAG 9 cut(s) 659, 758, 890, 2100, 2147, 2258, 2391, 2943, 3015
ZraI GACGTC 2 cut(s) 2691, 3412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.