Rh4DG130800

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005499
Physical Location & Seq
Forward (+)
21866989 .. 21882120
15132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG130800.1

Sequence Viewer

Length: 3264 bp
ATGAGAGCGGCGGCGTCTCTCCTTGTCAAATCAGTAGCCTCCTCCAACAATACTTGTTTTCGTTCTCACCACCTCAAATTCTGTCTTGTTGTTCTCCAACCACAATCACAACCACACTCTTCTTCTTCTTCCTCTTCTACCATTTCGATTTCGAAGCGCGGCTTTTCCGGCTACGCTGCGGAGCAGTTCTCCGACGACGAGTATGAGTGCGATTTCGAAAGCCAAAAGGCTTCATCTTCCGTGGCCAACATTGACGAGTGGAAATGGAAACTGAGCCTGCTTCTGCGCAGTGACAAGGACCAAGAGGTTGTTTCCAGAGATAAAAGGGATAGGAGAGACTATGAGCAAATATCTAACCTCGCCAAACGGATGGGACTTTACACTGAAATATATGGCAAAGTGGTGGTTGCCAGCAAGGTTCCTCTTCCAAACTACCGCCCCGATTTGGATGATAAGCGCCCACAAAGAGAGGTGGTTATCCCTCTTGGCTTGCAAAGGAGGGTAGAAGGTTTACTGCAAGAACACCTTGATCGTGTCCTGCTAAGTTCCCGCAACGTATCAGCCAATTCAGGTGGTGACAGTGACCACTTCGATCAGGTTGGAAATGAAAAGCCTGATGCGAATGCAGATTCTTTTCTTGATGGGTCTGTAATGGAGAAGGTTCTTCAGCGTAGGAGTTTGCGCATGCGTAATATGCAAAGAGCTTGGCAGGAATCACCTGAAGGTAAAAAGATGCTAGAGTTCCGGAAATCACTACCATCTTTCAAAGAGAATCAGAGGTTGCTGCAAGCAATAGCACAAAATCAGGTGGGCTATAAAGTTCGATTAGAGGGAATGAAAGGAAAAAATACGCATCTCCTTTTCTGTACCAGTGGTATTCTACTTCGCCGTCTGTTGAGTGATCGGAACTTGAGTGGTGTAACACATGTTTTTGTTGATGAAATTCATGAACGAGGCATGAATGAAGACTTCTTGTTGATTGTATTGAAAGATCTGCTTCCACGCCGTCGGGATCTAAGATTGGTTTTGATGAGTGCCACTCTGAATGCGGAGCTATTTTCAAATTTTTTTGGAGGAGCACCAACAATTCACATTCCGGGTTTCACATATCCAGTGAGAGCACTCTTTTTGGAAGATGTACTGGAAATGACTGGTTATAAGCTGACTTCTTTCAACCAAATTGATGACTATGGCCAAGACAAACTGTGGAAAACGCAGAAGCAGTTAGCGCCTCGGAAAAGGAAAAACCAGATCACTGCTCTAGTTGAGGATGCTCTTAGTAAATCGAGTTTTGAGAATTATAGCCCCAGAGCACGCGATTCATTATCATGTTGGACGGCTGATTGCATAGGATTTAATCTTATTGAAGCAGTTTTATGTCATATATGTCGGAAGGAACGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGAGGATATCAGCTGTTTGAGGGATCAACTTAAAGCCCATCCTCTTTTAGGTGATCCTAACAGGGTTTTGCTTATAACATGCCATGGGTCAATGACCACGTCTGAACAGAACCTCATTTTCGCGAGACCGCCTCCTAATGTACGTAAAATTGTTCTTGCTACTAATATGGCAGAAGCAAGCATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGGAAAGCAAAAGAGACCACATATGATGCTTTGAATAATACACCTTGTCTACTACCTTCTTGGATATCCCAAGCATCTGCTCGGCAAAGAAGGGGAAGGGCTGGCCGGGTGCAGCCAGGCGAATGTTACCACCTTTACCCCAAATGTGTGTATCATGCGTTTGCAGAATACCAACTTCCTGAACTCCTAAGGACTCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTTTGCAGGTTGGTAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCACCTGAACCCCTTGCTGTTCAAAATGCTGTTGGCTTTCTTAAGTTGATTGGGGCATTGGATGAGAATGAAATTTTGACAAATCTTGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTAAGTGTCCGGGATCCATTCCTTTTGCCTCAAGACAAAAAAGATTTAGCGGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTATAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCAGAAAGAGAAGGATCAGCATATGAATACTGCTGGAGAAATTTTCTCTCTGCCCAAACACTTCAAGCTATTCATTCTCTTCGGAAGCAGTTTAGCTACATCTTAAGAGACGCTGGCTTGGTGGATGCAGATGCAAGCATTAACAACAAGCTAAGTCACAATCAGTCATTGGTCCGCGCAATCATATGTTCCGGACTATTCCCTGGCATAGCATCTGTAGTGCACCGAGAGACATCCATGTCTTTCAAAACTATGGATGATGGCCAGGTTTTACTATATGCTAATTCTGTAAATGCACGTTACCAAACAATTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAGTAAAGGTCAACACAGTTTTCATACGCGATTCTACTGGTGTCTCTGATTCCATTTTGATCCTTTTTGGTGGTGCCCTAAACCATGGTATCCAGGCTGGGCATTTGAAAATGTTGGACGGCTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGCTTAAAGAAGAGCTTAATGAGCTTATTCAGAAGAAGCTTCAAGATCCAAGCCTCGATATCCACAAGGAAGGAAAGTATCTCATACTTGCTGTTCAAGAGCTGGTATCTGGAGATCAAAGTGAAGGAAGATTTGTGTTTGGCCGTGAAAACAGGAAGCCAAAGGAGTCCTGTGATAACAGCAGATTCACAAGGGATGGAACCAATCCTAAGAGTTTGCTACAAACTCTATTGATGAGAGCAGGACATTCACCTCCAAAATACAAAACCAAGCACCTCAAGACAAATGAGTTCAGAGCACTTGTGGAGTTCAAGGGCATGCAATTTGTTGGGAAGCCCAAGAAAAATAAGCAGCTTGCCGAAAGGGATGCTGCCATAGAAGCACTGGCCTGGTTGACTCACACCTCAGACAACAGTCGTGATGAAGATGACAACTCCCCACCTGATGTCACTAACAACATGCTGAAACTTCTGGGGAAACGTAGAAGATCAAAGCGCCACTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1087

Amino Acids

122.55

Weight (kDa)

8.96

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 458 - 587 3.4e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 651 - 679 1.7e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 680 - 740 5.5e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 807 - 888 5.1e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 897 - 955 2.4e-23 C-terminal domain in RNA helicases
dsrm PF00035 988 - 1050 7.2e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1158, 1508
AasI GACNNNNNNGTC 1 cut(s) 2485
Acc16I TGCGCA 2 cut(s) 287, 683
Acc36I ACCTGC 1 cut(s) 1873
AccB1I GGYRCC 1 cut(s) 2660
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 1699
AccII CGCG 5 cut(s) 159, 1317, 1556, 2424, 2616
AccIII TCCGGA 2 cut(s) 744, 2438
AcoI YGGCCR 6 cut(s) 243, 1192, 1400, 1753, 2509, 2896
AcsI RAATTY 7 cut(s) 77, 942, 1063, 1852, 1901, 1995, 2287
AcuI CTGAAG 2 cut(s) 650, 741
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 3 cut(s) 868, 1140, 1575
AfiI CCNNNNNNNGG 6 cut(s) 445, 1481, 2048, 2049, 2568, 2572
AflII CTTAAG 2 cut(s) 1964, 2350
AflIII ACRYGT 1 cut(s) 925
AjiI CACGTC 1 cut(s) 1533
AjnI CCWGG 6 cut(s) 1402, 1765, 2449, 2511, 2679, 3143
Alw21I GWGCWC 5 cut(s) 1081, 1123, 1315, 2472, 3055
Alw26I GTCTC 7 cut(s) 21, 330, 1552, 1658, 2349, 2471, 2635
Alw44I GTGCAC 1 cut(s) 2468
AlwNI CAGNNNCTG 1 cut(s) 1409
Aor13HI TCCGGA 2 cut(s) 744, 2438
AoxI GGCC 7 cut(s) 243, 1192, 1400, 1753, 2509, 2896, 3141
ApaLI GTGCAC 1 cut(s) 2468
ApeKI GCWGC 6 cut(s) 176, 784, 1762, 1913, 3106, 3125
ApoI RAATTY 7 cut(s) 77, 942, 1063, 1852, 1901, 1995, 2287
AspLEI GCGC 7 cut(s) 159, 288, 459, 684, 1231, 2426, 3252
AspS9I GGNCC 2 cut(s) 298, 2419
AsuC2I CCSGG 3 cut(s) 1098, 1757, 2126
AsuHPI GGTGA 6 cut(s) 59, 587, 708, 1496, 2594, 2997
AsuII TTCGAA 2 cut(s) 152, 216
AvaII GGWCC 2 cut(s) 298, 2419
AxyI CCTNAGG 1 cut(s) 1838
BaeGI GKGCMC 2 cut(s) 2472, 2665
BaeI ACNNNNGTAYC 2 cut(s) 2659, 2692
BalI TGGCCA 3 cut(s) 245, 1194, 2511
BamHI GGATCC 2 cut(s) 2128, 2728
BanI GGYRCC 1 cut(s) 2660
BbsI GAAGAC 1 cut(s) 972
Bbv12I GWGCWC 5 cut(s) 1081, 1123, 1315, 2472, 3055
BbvI GCAGC 6 cut(s) 163, 771, 1774, 1900, 3112, 3118
BccI CCATC 8 cut(s) 364, 635, 766, 1479, 2232, 2501, 2734, 2945
BceAI ACGGC 6 cut(s) 873, 990, 1353, 1415, 2722, 2883
BcgI CGANNNNNNTGC 2 cut(s) 3104, 3138
BciT130I CCWGG 6 cut(s) 1404, 1767, 2451, 2513, 2681, 3145
BciVI GTATCC 1 cut(s) 2687
BclI TGATCA 1 cut(s) 2206
BcnI CCSGG 3 cut(s) 1098, 1757, 2126
BcoDI GTCTC 7 cut(s) 21, 330, 1552, 1658, 2349, 2471, 2635
BfaI CTAG 5 cut(s) 737, 1262, 2048, 2181, 2733
BfmI CTRYAG 1 cut(s) 2463
BfoI RGCGCY 3 cut(s) 460, 1232, 3253
BfrI CTTAAG 2 cut(s) 1964, 2350
BfuAI ACCTGC 1 cut(s) 1873
BfuI GTATCC 1 cut(s) 2687
BglII AGATCT 1 cut(s) 991
BisI GCNGC 9 cut(s) 9, 12, 160, 177, 785, 1763, 1914, 3107, 3126
BlsI GCNGC 9 cut(s) 10, 13, 161, 178, 786, 1764, 1915, 3108, 3127
Bme1390I CCNGG 9 cut(s) 1098, 1404, 1757, 1767, 2126, 2451, 2513, 2681, 3145
Bme18I GGWCC 2 cut(s) 298, 2419
BmgBI CACGTC 1 cut(s) 1533
BmgT120I GGNCC 2 cut(s) 298, 2419
BmiI GGNNCC 5 cut(s) 420, 2130, 2662, 2730, 2956
BmrFI CCNGG 9 cut(s) 1098, 1404, 1757, 1767, 2126, 2451, 2513, 2681, 3145
BmrI ACTGGG 1 cut(s) 1437
BmuI ACTGGG 1 cut(s) 1437
BoxI GACNNNNGTC 1 cut(s) 3168
BpiI GAAGAC 1 cut(s) 972
BplI GAGNNNNNCTC 6 cut(s) 173, 205, 1024, 1056, 1549, 1581
BpmI CTGGAG 2 cut(s) 2302, 2886
Bpu14I TTCGAA 2 cut(s) 152, 216
BpuEI CTTGAG 3 cut(s) 931, 2130, 3017
BpuMI CCSGG 3 cut(s) 1098, 1757, 2126
BsaAI YACGTR 1 cut(s) 1577
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 2 cut(s) 1552, 1658
BsaJI CCNNGG 6 cut(s) 240, 1232, 1516, 2449, 2567, 2671
BsaWI WCCGGW 2 cut(s) 744, 2438
Bsc4I CCNNNNNNNGG 6 cut(s) 445, 1481, 2048, 2049, 2568, 2572
Bse1I ACTGG 8 cut(s) 870, 1112, 1146, 1156, 1432, 2033, 2629, 3144
Bse21I CCTNAGG 1 cut(s) 1838
Bse3DI GCAATG 1 cut(s) 2080
BseAI TCCGGA 2 cut(s) 744, 2438
BseBI CCWGG 6 cut(s) 1404, 1767, 2451, 2513, 2681, 3145
BseDI CCNNGG 6 cut(s) 240, 1232, 1516, 2449, 2567, 2671
BseLI CCNNNNNNNGG 6 cut(s) 445, 1481, 2048, 2049, 2568, 2572
BseMI GCAATG 1 cut(s) 2080
BseMII CTCAG 2 cut(s) 263, 3174
BseNI ACTGG 8 cut(s) 870, 1112, 1146, 1156, 1432, 2033, 2629, 3144
BseRI GAGGAG 2 cut(s) 31, 1089
BseSI GKGCMC 2 cut(s) 2472, 2665
BseXI GCAGC 6 cut(s) 163, 771, 1774, 1900, 3112, 3118
BseYI CCCAGC 1 cut(s) 2684
BsgI GTGCAG 1 cut(s) 1781
Bsh1236I CGCG 5 cut(s) 159, 1317, 1556, 2424, 2616
BshFI GGCC 7 cut(s) 245, 1194, 1402, 1755, 2511, 2898, 3143
BshNI GGYRCC 1 cut(s) 2660
BsiHKAI GWGCWC 5 cut(s) 1081, 1123, 1315, 2472, 3055
BsiSI CCGG 6 cut(s) 168, 745, 1097, 1756, 2125, 2439
BslFI GGGAC 2 cut(s) 387, 2182
BslI CCNNNNNNNGG 6 cut(s) 445, 1481, 2048, 2049, 2568, 2572
BsmAI GTCTC 7 cut(s) 21, 330, 1552, 1658, 2349, 2471, 2635
BsmBI CGTCTC 2 cut(s) 21, 2349
BsmFI GGGAC 2 cut(s) 387, 2182
BsmI GAATGC 2 cut(s) 628, 1051
BsnI GGCC 7 cut(s) 245, 1194, 1402, 1755, 2511, 2898, 3143
Bso31I GGTCTC 2 cut(s) 1552, 1658
Bsp119I TTCGAA 2 cut(s) 152, 216
Bsp1286I GDGCHC 6 cut(s) 1081, 1123, 1315, 2472, 2665, 3055
Bsp13I TCCGGA 2 cut(s) 744, 2438
Bsp19I CCATGG 2 cut(s) 1516, 2671
Bsp68I TCGCGA 1 cut(s) 1556
BspANI GGCC 7 cut(s) 245, 1194, 1402, 1755, 2511, 2898, 3143
BspCNI CTCAG 2 cut(s) 264, 3173
BspEI TCCGGA 2 cut(s) 744, 2438
BspFNI CGCG 5 cut(s) 159, 1317, 1556, 2424, 2616
BspHI TCATGA 2 cut(s) 946, 1422
BspLI GGNNCC 5 cut(s) 420, 2130, 2662, 2730, 2956
BspMI ACCTGC 1 cut(s) 1873
BspQI GCTCTTC 1 cut(s) 2760
BspT104I TTCGAA 2 cut(s) 152, 216
BspT107I GGYRCC 1 cut(s) 2660
BspTI CTTAAG 2 cut(s) 1964, 2350
BspTNI GGTCTC 2 cut(s) 1552, 1658
BsrBI CCGCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 2080
BsrI ACTGG 8 cut(s) 870, 1112, 1146, 1156, 1432, 2033, 2629, 3144
BssECI CCNNGG 6 cut(s) 240, 1232, 1516, 2449, 2567, 2671
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 3 cut(s) 1516, 2567, 2671
Bst2UI CCWGG 6 cut(s) 1404, 1767, 2451, 2513, 2681, 3145
Bst4CI ACNGT 4 cut(s) 581, 1206, 2605, 3170
Bst6I CTCTTC 5 cut(s) 124, 139, 429, 2331, 2760
BstACI GRCGYC 1 cut(s) 14
BstAFI CTTAAG 2 cut(s) 1964, 2350
BstBAI YACGTR 1 cut(s) 1577
BstBI TTCGAA 2 cut(s) 152, 216
BstDSI CCRYGG 3 cut(s) 240, 1516, 2671
BstENI CCTNNNNNAGG 1 cut(s) 1479
BstFNI CGCG 5 cut(s) 159, 1317, 1556, 2424, 2616
BstH2I RGCGCY 3 cut(s) 460, 1232, 3253
BstHHI GCGC 7 cut(s) 159, 288, 459, 684, 1231, 2426, 3252
BstMAI GTCTC 7 cut(s) 21, 330, 1552, 1658, 2349, 2471, 2635
BstMWI GCNNNNNNNGC 6 cut(s) 168, 694, 1228, 2171, 2776, 3134
BstNI CCWGG 6 cut(s) 1404, 1767, 2451, 2513, 2681, 3145
BstNSI RCATGY 5 cut(s) 688, 929, 1515, 3076, 3217
BstPAI GACNNNNGTC 1 cut(s) 3168
BstSCI CCNGG 9 cut(s) 1096, 1402, 1755, 1765, 2124, 2449, 2511, 2679, 3143
BstSFI CTRYAG 1 cut(s) 2463
BstSLI GKGCMC 2 cut(s) 2472, 2665
BstSNI TACGTA 1 cut(s) 1577
BstUI CGCG 5 cut(s) 159, 1317, 1556, 2424, 2616
BstV1I GCAGC 6 cut(s) 163, 771, 1774, 1900, 3112, 3118
BstV2I GAAGAC 1 cut(s) 972
BstX2I RGATCY 6 cut(s) 991, 1012, 2038, 2128, 2728, 2800
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 6 cut(s) 991, 1012, 2038, 2128, 2728, 2800
Bsu36I CCTNAGG 1 cut(s) 1838
BsuI GTATCC 1 cut(s) 2687
BsuRI GGCC 7 cut(s) 245, 1194, 1402, 1755, 2511, 2898, 3143
BtgI CCRYGG 3 cut(s) 240, 1516, 2671
BtrI CACGTC 1 cut(s) 1533
BtsI GCAGTG 2 cut(s) 295, 1254
BtsIMutI CAGTG 7 cut(s) 295, 381, 586, 877, 1119, 1254, 3137
BtuMI TCGCGA 1 cut(s) 1556
BveI ACCTGC 1 cut(s) 1873
CaiI CAGNNNCTG 1 cut(s) 1409
CciI TCATGA 2 cut(s) 946, 1422
CfoI GCGC 7 cut(s) 159, 288, 459, 684, 1231, 2426, 3252
Cfr13I GGNCC 2 cut(s) 298, 2419
CseI GACGC 2 cut(s) 3, 2366
Csp6I GTAC 3 cut(s) 867, 1139, 1574
CspCI CAANNNNNGTGG 2 cut(s) 553, 588
CviQI GTAC 3 cut(s) 867, 1139, 1574
DrdI GACNNNNNNGTC 1 cut(s) 2485
DseDI GACNNNNNNGTC 1 cut(s) 2485
EaeI YGGCCR 6 cut(s) 243, 1192, 1400, 1753, 2509, 2896
Eam1104I CTCTTC 5 cut(s) 124, 139, 429, 2331, 2760
EarI CTCTTC 5 cut(s) 124, 139, 429, 2331, 2760
Eco105I TACGTA 1 cut(s) 1577
Eco130I CCWWGG 3 cut(s) 1516, 2567, 2671
Eco31I GGTCTC 2 cut(s) 1552, 1658
Eco32I GATATC 3 cut(s) 1441, 1716, 2815
Eco47I GGWCC 2 cut(s) 298, 2419
Eco57I CTGAAG 2 cut(s) 650, 741
Eco81I CCTNAGG 1 cut(s) 1838
EcoNI CCTNNNNNAGG 1 cut(s) 1479
EcoRI GAATTC 2 cut(s) 1852, 1901
EcoRII CCWGG 6 cut(s) 1402, 1765, 2449, 2511, 2679, 3143
EcoRV GATATC 3 cut(s) 1441, 1716, 2815
EcoT14I CCWWGG 3 cut(s) 1516, 2567, 2671
ErhI CCWWGG 3 cut(s) 1516, 2567, 2671
Esp3I CGTCTC 2 cut(s) 21, 2349
FaqI GGGAC 2 cut(s) 387, 2182
FauI CCCGC 2 cut(s) 557, 2158
FauNDI CATATG 5 cut(s) 1672, 2211, 2230, 2269, 2432
FbaI TGATCA 1 cut(s) 2206
FblI GTMKAC 1 cut(s) 1699
Fnu4HI GCNGC 9 cut(s) 9, 12, 160, 177, 785, 1763, 1914, 3107, 3126
Fsp4HI GCNGC 9 cut(s) 9, 12, 160, 177, 785, 1763, 1914, 3107, 3126
FspBI CTAG 5 cut(s) 737, 1262, 2048, 2181, 2733
FspI TGCGCA 2 cut(s) 287, 683
GlaI GCGC 7 cut(s) 158, 287, 458, 683, 1230, 2425, 3251
GluI GCNGC 9 cut(s) 9, 12, 160, 177, 785, 1763, 1914, 3107, 3126
GsaI CCCAGC 1 cut(s) 2688
GsuI CTGGAG 2 cut(s) 2302, 2886
HaeII RGCGCY 3 cut(s) 460, 1232, 3253
HaeIII GGCC 7 cut(s) 245, 1194, 1402, 1755, 2511, 2898, 3143
HapII CCGG 6 cut(s) 168, 745, 1097, 1756, 2125, 2439
HgaI GACGC 2 cut(s) 3, 2366
HhaI GCGC 7 cut(s) 159, 288, 459, 684, 1231, 2426, 3252
Hin1I GRCGYC 1 cut(s) 14
Hin6I GCGC 7 cut(s) 157, 286, 457, 682, 1229, 2424, 3250
HinP1I GCGC 7 cut(s) 157, 286, 457, 682, 1229, 2424, 3250
HincII GTYRAC 2 cut(s) 2599, 3150
HindII GTYRAC 2 cut(s) 2599, 3150
HindIII AAGCTT 2 cut(s) 2756, 2792
HpaII CCGG 6 cut(s) 168, 745, 1097, 1756, 2125, 2439
HphI GGTGA 6 cut(s) 59, 587, 708, 1496, 2594, 2997
Hpy166II GTNNAC 5 cut(s) 512, 1700, 2470, 2599, 3150
Hpy8I GTNNAC 5 cut(s) 512, 1700, 2470, 2599, 3150
Hpy99I CGWCG 3 cut(s) 197, 200, 1011
HpyCH4III ACNGT 4 cut(s) 581, 1206, 2605, 3170
HpyCH4IV ACGT 5 cut(s) 555, 1532, 1576, 2545, 3235
HpyF10VI GCNNNNNNNGC 6 cut(s) 168, 694, 1228, 2171, 2776, 3134
HpySE526I ACGT 5 cut(s) 555, 1532, 1576, 2545, 3235
Hsp92I GRCGYC 1 cut(s) 14
HspAI GCGC 7 cut(s) 157, 286, 457, 682, 1229, 2424, 3250
Kpn2I TCCGGA 2 cut(s) 744, 2438
Ksp22I TGATCA 1 cut(s) 2206
LguI GCTCTTC 1 cut(s) 2760
LmnI GCTCC 3 cut(s) 181, 1051, 1076
Lsp1109I GCAGC 6 cut(s) 163, 771, 1774, 1900, 3112, 3118
MaeI CTAG 5 cut(s) 737, 1262, 2048, 2181, 2733
MaeII ACGT 5 cut(s) 555, 1532, 1576, 2545, 3235
MaeIII GTNAC 8 cut(s) 290, 575, 581, 919, 1775, 2402, 2546, 3202
MbiI CCGCTC 1 cut(s) 8
MflI RGATCY 6 cut(s) 991, 1012, 2038, 2128, 2728, 2800
MhlI GDGCHC 6 cut(s) 1081, 1123, 1315, 2472, 2665, 3055
MlsI TGGCCA 3 cut(s) 245, 1194, 2511
MluNI TGGCCA 3 cut(s) 245, 1194, 2511
MlyI GAGTC 3 cut(s) 1837, 2930, 3145
MmeI TCCRAC 7 cut(s) 69, 121, 216, 580, 1313, 1370, 2682
Mox20I TGGCCA 3 cut(s) 245, 1194, 2511
MroI TCCGGA 2 cut(s) 744, 2438
MscI TGGCCA 3 cut(s) 245, 1194, 2511
MseI TTAA 8 cut(s) 1356, 1464, 1965, 2351, 2388, 2760, 2772, 3262
MslI CAYNNNNRTG 1 cut(s) 1327
Msp20I TGGCCA 3 cut(s) 245, 1194, 2511
MspA1I CMGCKG 2 cut(s) 1446, 1913
MspCI CTTAAG 2 cut(s) 1964, 2350
MspI CCGG 6 cut(s) 168, 745, 1097, 1756, 2125, 2439
MspR9I CCNGG 9 cut(s) 1098, 1404, 1757, 1767, 2126, 2451, 2513, 2681, 3145
Mva1269I GAATGC 2 cut(s) 628, 1051
MvaI CCWGG 6 cut(s) 1404, 1767, 2451, 2513, 2681, 3145
MvnI CGCG 5 cut(s) 159, 1317, 1556, 2424, 2616
MwoI GCNNNNNNNGC 6 cut(s) 168, 694, 1228, 2171, 2776, 3134
NciI CCSGG 3 cut(s) 1098, 1757, 2126
NcoI CCATGG 2 cut(s) 1516, 2671
NdeI CATATG 5 cut(s) 1672, 2211, 2230, 2269, 2432
NlaIV GGNNCC 5 cut(s) 420, 2130, 2662, 2730, 2956
NmeAIII GCCGAG 1 cut(s) 1711
NmuCI GTSAC 5 cut(s) 290, 575, 581, 2402, 3202
NruI TCGCGA 1 cut(s) 1556
NsbI TGCGCA 2 cut(s) 287, 683
NspI RCATGY 5 cut(s) 688, 929, 1515, 3076, 3217
NspV TTCGAA 2 cut(s) 152, 216
PaeI GCATGC 2 cut(s) 688, 3076
PagI TCATGA 2 cut(s) 946, 1422
PciI ACATGT 1 cut(s) 925
PciSI GCTCTTC 1 cut(s) 2760
PctI GAATGC 2 cut(s) 628, 1051
PfeI GAWTC 8 cut(s) 629, 713, 772, 1319, 2184, 2618, 2636, 2940
PflFI GACNNNGTC 1 cut(s) 1531
PfoI TCCNGGA 1 cut(s) 2124
PkrI GCNGC 9 cut(s) 10, 13, 161, 178, 786, 1764, 1915, 3108, 3127
PleI GAGTC 3 cut(s) 1837, 2929, 3145
PpsI GAGTC 3 cut(s) 1837, 2929, 3145
Ppu21I YACGTR 1 cut(s) 1577
PscI ACATGT 1 cut(s) 925
PshAI GACNNNNGTC 1 cut(s) 3168
PsiI TTATAA 2 cut(s) 1158, 1508
Psp6I CCWGG 6 cut(s) 1402, 1765, 2449, 2511, 2679, 3143
PspFI CCCAGC 1 cut(s) 2684
PspGI CCWGG 6 cut(s) 1402, 1765, 2449, 2511, 2679, 3143
PspN4I GGNNCC 5 cut(s) 420, 2130, 2662, 2730, 2956
PspPI GGNCC 2 cut(s) 298, 2419
PstNI CAGNNNCTG 1 cut(s) 1409
PsuI RGATCY 6 cut(s) 991, 1012, 2038, 2128, 2728, 2800
PsyI GACNNNGTC 1 cut(s) 1531
PvuII CAGCTG 2 cut(s) 1446, 1913
RruI TCGCGA 1 cut(s) 1556
RsaI GTAC 3 cut(s) 868, 1140, 1575
RsaNI GTAC 3 cut(s) 867, 1139, 1574
RseI CAYNNNNRTG 1 cut(s) 1327
SapI GCTCTTC 1 cut(s) 2760
SaqAI TTAA 8 cut(s) 1356, 1464, 1965, 2351, 2388, 2760, 2772, 3262
SatI GCNGC 9 cut(s) 9, 12, 160, 177, 785, 1763, 1914, 3107, 3126
Sau96I GGNCC 2 cut(s) 298, 2419
SchI GAGTC 3 cut(s) 1837, 2930, 3145
ScrFI CCNGG 9 cut(s) 1098, 1404, 1757, 1767, 2126, 2451, 2513, 2681, 3145
SduI GDGCHC 6 cut(s) 1081, 1123, 1315, 2472, 2665, 3055
SfcI CTRYAG 1 cut(s) 2463
SfuI TTCGAA 2 cut(s) 152, 216
SinI GGWCC 2 cut(s) 298, 2419
SmiMI CAYNNNNRTG 1 cut(s) 1327
SmlI CTYRAG 5 cut(s) 910, 1964, 2145, 2350, 3032
SmoI CTYRAG 5 cut(s) 910, 1964, 2145, 2350, 3032
SnaBI TACGTA 1 cut(s) 1577
SphI GCATGC 2 cut(s) 688, 3076
SspI AATATT 1 cut(s) 2018
SspMI CTAG 5 cut(s) 737, 1262, 2048, 2181, 2733
StyD4I CCNGG 9 cut(s) 1096, 1402, 1755, 1765, 2124, 2449, 2511, 2679, 3143
StyI CCWWGG 3 cut(s) 1516, 2567, 2671
TaaI ACNGT 4 cut(s) 581, 1206, 2605, 3170
TaiI ACGT 5 cut(s) 558, 1535, 1579, 2548, 3238
TaqI TCGA 7 cut(s) 146, 152, 216, 591, 823, 1286, 2811
TatI WGTACW 1 cut(s) 1138
TauI GCSGC 3 cut(s) 11, 14, 162
TfiI GAWTC 8 cut(s) 629, 713, 772, 1319, 2184, 2618, 2636, 2940
Tru1I TTAA 8 cut(s) 1356, 1464, 1965, 2351, 2388, 2760, 2772, 3262
Tru9I TTAA 8 cut(s) 1356, 1464, 1965, 2351, 2388, 2760, 2772, 3262
TscAI CASTG 7 cut(s) 295, 388, 586, 877, 1119, 1261, 3144
TseFI GTSAC 5 cut(s) 290, 575, 581, 2402, 3202
TseI GCWGC 6 cut(s) 176, 784, 1762, 1913, 3106, 3125
Tsp45I GTSAC 5 cut(s) 290, 575, 581, 2402, 3202
TspGWI ACGGA 2 cut(s) 229, 382
TspRI CASTG 7 cut(s) 295, 388, 586, 877, 1119, 1261, 3144
Tth111I GACNNNGTC 1 cut(s) 1531
Vha464I CTTAAG 2 cut(s) 1964, 2350
VneI GTGCAC 1 cut(s) 2468
VpaK11BI GGWCC 2 cut(s) 298, 2419
XagI CCTNNNNNAGG 1 cut(s) 1479
XapI RAATTY 7 cut(s) 77, 942, 1063, 1852, 1901, 1995, 2287
XbaI TCTAGA 1 cut(s) 2180
XceI RCATGY 5 cut(s) 688, 929, 1515, 3076, 3217
XcmI CCANNNNNNNNNTGG 1 cut(s) 3136
XmiI GTMKAC 1 cut(s) 1699
XspI CTAG 5 cut(s) 737, 1262, 2048, 2181, 2733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.