Rh5CG366800

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005499
Physical Location & Seq
Forward (+)
44707937 .. 44726842
18906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG366800.1

Sequence Viewer

Length: 2061 bp
ATGAAAGGAAAAAATACGCATCTCCTTTTCGGTACCAGTGGTATTCTACTTCGCCGTCTGTTGAGTGATAGGAACTTGAGTGGTGTAACACATGTTTTTGTTGATGAAATTCATGAACGAGGCATGAATGAAGACTTCTTGTTGATTGTATTGAAAGATCTGCTTCCACGCCGTCGGGATCTAAGATTGGTTTTGATGAGTGCCACTCTGAATGCGGAGCTATTTTCAAATTTTTTTGGAGGAGCACCAACAATTCACATTCCGGGTTTCACATATCCAATGAAAGCACACTTTTTGGAAGATGTACTGGAAATGACTGGGTATAAGCTGACTTCTTTCAACCAAATTGATGACTATGGCCAAGACAAACTGTGGAAAATGCAGAAGCAGTTAGCGCCTCGGAAAAGGAAAAACCAGATCACTGCTCTAGTTGAGGATGCTCTTAGTAAATCAAGCTTTGAGAATTATAGCCCCAGAGCACGTGATTCATTATCATGTTGGACAGCTGATTGCATAGGATTTAATCTTATTGAAGCAGTTTTATGTCATATATGTCGGAAGGAACGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGAGGATATCAGCTCTTTGAGGGATCAACTTAAAGCCCATCCTCTTTTAGGTGATCCTAACAGGGTTTTGCTTATAACATGCCATGGGTCAATGGCCACGTCTGAACAGAACCTCATTTTTGGGAGACCGCCTCCTAATGTACGTAAAATAGTTCTTGCTACTAATATGGCAGAAGCAAGCATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCACATATGATGCTTTGAATAATACACCTTGTCTACTACCTTCTTGGATATCCCAAGCATCTGCTCGGCAAAGAAGGGGAAGGGCTGGCCGGGTGCAGCCAGGCGAATGTTACCACCTTTACCCCAAATGTGTGTATCATGCTTTTGCGGAATACCAACTTCCTGAACTTCTAAGGACGCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTTTGCAGGTTGGTAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCACCTGAACCCCTTGCTGTTCAAAATGCTGTTGGCTTTCTTAAGTTGATTGAGGCATTGGATGAGAATGAAAATTTGACAAATCTCGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTCAGTGTCCGGGATCCATTCCTTTTGCCTCAAGACAAAAAAGATAATTCTGTAAATGCACGTTACCAAACAATTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAGTAAAGGTCAACACTGTTTTCATACGCGATTCTACTGGTGTCTCTGATTCTGTTTTGATCCTTTTTGGCGGTGCCCTAATCCATGGGATCCAGGCTGGGCATTTGAAAATGTTGGACGGCTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGCTTAAAGAGGAGCTTAATGAGCTTATTCAGAAGAAGCTTCAAGATCCAAGCCTCGATATCCACAAGGAAGGAAAGTATCTCATACTTGCTGTTCAAGAGCTAGTATCTGGAGATCAAAGTGAAGGAAGATTTGTGTTTGGCCGTGATAACAGGAAGCCAAAGGAGTCTGGTGATAACAGCAGATTCACAAGGGATGAAACCAATCCTAAGAGTTTGCTACAAACTTTATTGATGAGAGCAGGACATTCACCTCCAAAATACAAAACCAAGCACCTCAAGACAAATGAGTTCAGAGAACTAGTGGAGTTCAAGGGCATGCAATTTGTTGGGAAGCCCATGAAAAATAAGCAGCTTGTCGAAAGGGATGCTGCCATAGAAGCACTGGCCTGGTTCACTCACACCTCAGACAACAGTCGTGATGAAGATGACAACTCCCCACCTGATGTTACTAACAACATGCTGAAACTTCTGGGGAAACGTAGAAGATCGAAGCGCCACTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

686

Amino Acids

77.36

Weight (kDa)

8.7

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 180 - 309 1.5e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 373 - 401 1.3e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 402 - 445 9.5e-13 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 443 - 487 1.3e-07 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 496 - 554 1.4e-23 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 674
Acc36I ACCTGC 1 cut(s) 1039
Acc65I GGTACC 1 cut(s) 32
AccB1I GGYRCC 2 cut(s) 32, 1457
AccI GTMKAC 1 cut(s) 865
AccII CGCG 1 cut(s) 1413
AciI CCGC 4 cut(s) 215, 728, 980, 1455
AcoI YGGCCR 5 cut(s) 358, 566, 693, 919, 1693
AcsI RAATTY 5 cut(s) 108, 229, 1018, 1067, 1162
AcvI CACGTG 1 cut(s) 482
AcyI GRCGYC 1 cut(s) 1010
AfaI GTAC 3 cut(s) 34, 306, 741
AfiI CCNNNNNNNGG 5 cut(s) 647, 1214, 1215, 1365, 1369
AflII CTTAAG 1 cut(s) 1130
AflIII ACRYGT 1 cut(s) 91
AhlI ACTAGT 1 cut(s) 1852
AjiI CACGTC 1 cut(s) 699
AjnI CCWGG 4 cut(s) 568, 931, 1476, 1940
Alw21I GWGCWC 2 cut(s) 247, 481
Alw26I GTCTC 3 cut(s) 718, 824, 1432
AlwNI CAGNNNCTG 1 cut(s) 575
AoxI GGCC 6 cut(s) 358, 566, 693, 919, 1693, 1938
ApeKI GCWGC 4 cut(s) 928, 1079, 1903, 1922
ApoI RAATTY 5 cut(s) 108, 229, 1018, 1067, 1162
Asp718I GGTACC 1 cut(s) 32
AspLEI GCGC 2 cut(s) 397, 2049
AsuC2I CCSGG 3 cut(s) 264, 923, 1292
AsuHPI GGTGA 4 cut(s) 662, 1391, 1736, 1794
BaeGI GKGCMC 1 cut(s) 1462
BalI TGGCCA 2 cut(s) 360, 695
BamHI GGATCC 3 cut(s) 1294, 1473, 1525
BanI GGYRCC 2 cut(s) 32, 1457
BanII GRGCYC 1 cut(s) 1284
BbrPI CACGTG 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 2 cut(s) 247, 481
BbvI GCAGC 4 cut(s) 940, 1066, 1909, 1915
BccI CCATC 2 cut(s) 645, 1531
BceAI ACGGC 5 cut(s) 39, 156, 581, 1519, 1680
BcgI CGANNNNNNTGC 2 cut(s) 1901, 1935
BciT130I CCWGG 4 cut(s) 570, 933, 1478, 1942
BcnI CCSGG 3 cut(s) 264, 923, 1292
BcoDI GTCTC 3 cut(s) 718, 824, 1432
BcuI ACTAGT 1 cut(s) 1852
BfaI CTAG 5 cut(s) 428, 1214, 1530, 1655, 1853
BfoI RGCGCY 2 cut(s) 398, 2050
BfrI CTTAAG 1 cut(s) 1130
BfuAI ACCTGC 1 cut(s) 1039
BglII AGATCT 1 cut(s) 157
BisI GCNGC 4 cut(s) 929, 1080, 1904, 1923
BlsI GCNGC 4 cut(s) 930, 1081, 1905, 1924
Bme1390I CCNGG 7 cut(s) 264, 570, 923, 933, 1292, 1478, 1942
BmgBI CACGTC 1 cut(s) 699
BmiI GGNNCC 5 cut(s) 34, 1296, 1459, 1475, 1527
BmrFI CCNGG 7 cut(s) 264, 570, 923, 933, 1292, 1478, 1942
BmrI ACTGGG 2 cut(s) 327, 603
BmsI GCATC 5 cut(s) 28, 427, 832, 899, 1909
BmuI ACTGGG 2 cut(s) 327, 603
BoxI GACNNNNGTC 1 cut(s) 1965
BpiI GAAGAC 1 cut(s) 138
BplI GAGNNNNNCTC 4 cut(s) 190, 222, 715, 747
BpmI CTGGAG 1 cut(s) 1683
BpuEI CTTGAG 3 cut(s) 97, 1296, 1814
BpuMI CCSGG 3 cut(s) 264, 923, 1292
BsaAI YACGTR 2 cut(s) 482, 743
BsaHI GRCGYC 1 cut(s) 1010
BsaI GGTCTC 2 cut(s) 718, 824
BsaJI CCNNGG 4 cut(s) 398, 682, 1364, 1468
Bsc4I CCNNNNNNNGG 5 cut(s) 647, 1214, 1215, 1365, 1369
Bse1I ACTGG 7 cut(s) 36, 312, 322, 598, 1199, 1426, 1941
Bse3DI GCAATG 1 cut(s) 1246
BseBI CCWGG 4 cut(s) 570, 933, 1478, 1942
BseDI CCNNGG 4 cut(s) 398, 682, 1364, 1468
BseGI GGATG 5 cut(s) 442, 637, 1156, 1753, 1924
BseLI CCNNNNNNNGG 5 cut(s) 647, 1214, 1215, 1365, 1369
BseMI GCAATG 1 cut(s) 1246
BseMII CTCAG 2 cut(s) 1297, 1971
BseNI ACTGG 7 cut(s) 36, 312, 322, 598, 1199, 1426, 1941
BseRI GAGGAG 2 cut(s) 255, 1577
BseSI GKGCMC 1 cut(s) 1462
BseXI GCAGC 4 cut(s) 940, 1066, 1909, 1915
BseYI CCCAGC 1 cut(s) 1481
BsgI GTGCAG 1 cut(s) 947
Bsh1236I CGCG 1 cut(s) 1413
BshFI GGCC 6 cut(s) 360, 568, 695, 921, 1695, 1940
BshNI GGYRCC 2 cut(s) 32, 1457
BsiHKAI GWGCWC 2 cut(s) 247, 481
BsiSI CCGG 3 cut(s) 263, 922, 1291
BslI CCNNNNNNNGG 5 cut(s) 647, 1214, 1215, 1365, 1369
BsmAI GTCTC 3 cut(s) 718, 824, 1432
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 6 cut(s) 360, 568, 695, 921, 1695, 1940
Bso31I GGTCTC 2 cut(s) 718, 824
Bsp1286I GDGCHC 4 cut(s) 247, 481, 1284, 1462
Bsp19I CCATGG 2 cut(s) 682, 1468
BspACI CCGC 4 cut(s) 215, 728, 980, 1455
BspANI GGCC 6 cut(s) 360, 568, 695, 921, 1695, 1940
BspCNI CTCAG 2 cut(s) 1296, 1970
BspFNI CGCG 1 cut(s) 1413
BspHI TCATGA 2 cut(s) 112, 588
BspLI GGNNCC 5 cut(s) 34, 1296, 1459, 1475, 1527
BspMI ACCTGC 1 cut(s) 1039
BspT107I GGYRCC 2 cut(s) 32, 1457
BspTI CTTAAG 1 cut(s) 1130
BspTNI GGTCTC 2 cut(s) 718, 824
BsrDI GCAATG 1 cut(s) 1246
BsrI ACTGG 7 cut(s) 36, 312, 322, 598, 1199, 1426, 1941
BssECI CCNNGG 4 cut(s) 398, 682, 1364, 1468
BssNI GRCGYC 1 cut(s) 1010
BssT1I CCWWGG 3 cut(s) 682, 1364, 1468
Bst2UI CCWGG 4 cut(s) 570, 933, 1478, 1942
Bst4CI ACNGT 3 cut(s) 372, 1402, 1967
BstACI GRCGYC 1 cut(s) 1010
BstAFI CTTAAG 1 cut(s) 1130
BstBAI YACGTR 2 cut(s) 482, 743
BstC8I GCNNGC 4 cut(s) 778, 919, 1031, 1871
BstDEI CTNAG 7 cut(s) 182, 443, 580, 1004, 1283, 1761, 1957
BstDSI CCRYGG 2 cut(s) 682, 1468
BstENI CCTNNNNNAGG 1 cut(s) 645
BstF5I GGATG 5 cut(s) 442, 637, 1156, 1753, 1924
BstFNI CGCG 1 cut(s) 1413
BstH2I RGCGCY 2 cut(s) 398, 2050
BstHHI GCGC 2 cut(s) 397, 2049
BstMAI GTCTC 3 cut(s) 718, 824, 1432
BstMWI GCNNNNNNNGC 3 cut(s) 394, 1573, 1931
BstNI CCWGG 4 cut(s) 570, 933, 1478, 1942
BstNSI RCATGY 4 cut(s) 95, 681, 1873, 2014
BstPAI GACNNNNGTC 1 cut(s) 1965
BstSCI CCNGG 7 cut(s) 262, 568, 921, 931, 1290, 1476, 1940
BstSLI GKGCMC 1 cut(s) 1462
BstSNI TACGTA 1 cut(s) 743
BstUI CGCG 1 cut(s) 1413
BstV1I GCAGC 4 cut(s) 940, 1066, 1909, 1915
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 7 cut(s) 157, 178, 1204, 1294, 1473, 1525, 1597
BstYI RGATCY 7 cut(s) 157, 178, 1204, 1294, 1473, 1525, 1597
BsuRI GGCC 6 cut(s) 360, 568, 695, 921, 1695, 1940
BtgI CCRYGG 2 cut(s) 682, 1468
BtrI CACGTC 1 cut(s) 699
BtsCI GGATG 5 cut(s) 442, 637, 1156, 1753, 1924
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 5 cut(s) 43, 420, 1291, 1398, 1934
BveI ACCTGC 1 cut(s) 1039
Cac8I GCNNGC 4 cut(s) 778, 919, 1031, 1871
CaiI CAGNNNCTG 1 cut(s) 575
CciI TCATGA 2 cut(s) 112, 588
CfoI GCGC 2 cut(s) 397, 2049
CseI GACGC 1 cut(s) 1018
Csp6I GTAC 3 cut(s) 33, 305, 740
CviQI GTAC 3 cut(s) 33, 305, 740
DdeI CTNAG 7 cut(s) 182, 443, 580, 1004, 1283, 1761, 1957
EaeI YGGCCR 5 cut(s) 358, 566, 693, 919, 1693
Eco105I TACGTA 1 cut(s) 743
Eco130I CCWWGG 3 cut(s) 682, 1364, 1468
Eco24I GRGCYC 1 cut(s) 1284
Eco31I GGTCTC 2 cut(s) 718, 824
Eco32I GATATC 3 cut(s) 607, 882, 1612
Eco72I CACGTG 1 cut(s) 482
EcoNI CCTNNNNNAGG 1 cut(s) 645
EcoRI GAATTC 2 cut(s) 1018, 1067
EcoRII CCWGG 4 cut(s) 568, 931, 1476, 1940
EcoRV GATATC 3 cut(s) 607, 882, 1612
EcoT14I CCWWGG 3 cut(s) 682, 1364, 1468
EcoT38I GRGCYC 1 cut(s) 1284
ErhI CCWWGG 3 cut(s) 682, 1364, 1468
FalI AAGNNNNNCTT 6 cut(s) 147, 179, 997, 1029, 1551, 1583
FauNDI CATATG 1 cut(s) 838
FblI GTMKAC 1 cut(s) 865
Fnu4HI GCNGC 4 cut(s) 929, 1080, 1904, 1923
FokI GGATG 5 cut(s) 449, 624, 1163, 1760, 1931
FriOI GRGCYC 1 cut(s) 1284
Fsp4HI GCNGC 4 cut(s) 929, 1080, 1904, 1923
FspBI CTAG 5 cut(s) 428, 1214, 1530, 1655, 1853
GlaI GCGC 2 cut(s) 396, 2048
GluI GCNGC 4 cut(s) 929, 1080, 1904, 1923
GsaI CCCAGC 1 cut(s) 1485
GsuI CTGGAG 1 cut(s) 1683
HaeII RGCGCY 2 cut(s) 398, 2050
HaeIII GGCC 6 cut(s) 360, 568, 695, 921, 1695, 1940
HapII CCGG 3 cut(s) 263, 922, 1291
HgaI GACGC 1 cut(s) 1018
HhaI GCGC 2 cut(s) 397, 2049
Hin1I GRCGYC 1 cut(s) 1010
Hin6I GCGC 2 cut(s) 395, 2047
HinP1I GCGC 2 cut(s) 395, 2047
HincII GTYRAC 1 cut(s) 1396
HindII GTYRAC 1 cut(s) 1396
HindIII AAGCTT 3 cut(s) 454, 1553, 1589
HinfI GANTC 5 cut(s) 485, 1415, 1433, 1718, 1737
HpaII CCGG 3 cut(s) 263, 922, 1291
HphI GGTGA 4 cut(s) 662, 1391, 1736, 1794
Hpy166II GTNNAC 3 cut(s) 866, 1396, 1947
Hpy188I TCNGA 9 cut(s) 210, 402, 558, 703, 1178, 1432, 1584, 1847, 1960
Hpy8I GTNNAC 3 cut(s) 866, 1396, 1947
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 6 cut(s) 553, 882, 900, 906, 1616, 1670
HpyCH4III ACNGT 3 cut(s) 372, 1402, 1967
HpyCH4IV ACGT 5 cut(s) 481, 698, 742, 1342, 2032
HpyCH4V TGCA 8 cut(s) 382, 513, 928, 1033, 1048, 1087, 1340, 1873
HpyF10VI GCNNNNNNNGC 3 cut(s) 394, 1573, 1931
HpyF3I CTNAG 7 cut(s) 182, 443, 580, 1004, 1283, 1761, 1957
HpySE526I ACGT 5 cut(s) 481, 698, 742, 1342, 2032
Hsp92I GRCGYC 1 cut(s) 1010
HspAI GCGC 2 cut(s) 395, 2047
KpnI GGTACC 1 cut(s) 36
LmnI GCTCC 3 cut(s) 217, 242, 1564
Lsp1109I GCAGC 4 cut(s) 940, 1066, 1909, 1915
LweI GCATC 5 cut(s) 28, 427, 832, 899, 1909
MaeI CTAG 5 cut(s) 428, 1214, 1530, 1655, 1853
MaeII ACGT 5 cut(s) 481, 698, 742, 1342, 2032
MaeIII GTNAC 4 cut(s) 85, 941, 1343, 1999
MboII GAAGA 7 cut(s) 143, 311, 1510, 1597, 1692, 1988, 2049
MflI RGATCY 7 cut(s) 157, 178, 1204, 1294, 1473, 1525, 1597
MhlI GDGCHC 4 cut(s) 247, 481, 1284, 1462
MlsI TGGCCA 2 cut(s) 360, 695
MluNI TGGCCA 2 cut(s) 360, 695
MlyI GAGTC 1 cut(s) 1727
MmeI TCCRAC 3 cut(s) 479, 536, 1479
Mox20I TGGCCA 2 cut(s) 360, 695
MscI TGGCCA 2 cut(s) 360, 695
MseI TTAA 6 cut(s) 522, 630, 1131, 1557, 1569, 2059
MslI CAYNNNNRTG 1 cut(s) 493
Msp20I TGGCCA 2 cut(s) 360, 695
MspA1I CMGCKG 2 cut(s) 506, 1079
MspCI CTTAAG 1 cut(s) 1130
MspI CCGG 3 cut(s) 263, 922, 1291
MspR9I CCNGG 7 cut(s) 264, 570, 923, 933, 1292, 1478, 1942
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 4 cut(s) 570, 933, 1478, 1942
MvnI CGCG 1 cut(s) 1413
MwoI GCNNNNNNNGC 3 cut(s) 394, 1573, 1931
NciI CCSGG 3 cut(s) 264, 923, 1292
NcoI CCATGG 2 cut(s) 682, 1468
NdeI CATATG 1 cut(s) 838
NlaIV GGNNCC 5 cut(s) 34, 1296, 1459, 1475, 1527
NmeAIII GCCGAG 1 cut(s) 877
NspI RCATGY 4 cut(s) 95, 681, 1873, 2014
PaeI GCATGC 1 cut(s) 1873
PagI TCATGA 2 cut(s) 112, 588
PciI ACATGT 1 cut(s) 91
PctI GAATGC 1 cut(s) 217
PfeI GAWTC 4 cut(s) 485, 1415, 1433, 1737
PfoI TCCNGGA 1 cut(s) 1290
PkrI GCNGC 4 cut(s) 930, 1081, 1905, 1924
PleI GAGTC 1 cut(s) 1726
PmaCI CACGTG 1 cut(s) 482
PmlI CACGTG 1 cut(s) 482
PpsI GAGTC 1 cut(s) 1726
Ppu21I YACGTR 2 cut(s) 482, 743
PscI ACATGT 1 cut(s) 91
PshAI GACNNNNGTC 1 cut(s) 1965
PsiI TTATAA 1 cut(s) 674
Psp6I CCWGG 4 cut(s) 568, 931, 1476, 1940
PspCI CACGTG 1 cut(s) 482
PspFI CCCAGC 1 cut(s) 1481
PspGI CCWGG 4 cut(s) 568, 931, 1476, 1940
PspN4I GGNNCC 5 cut(s) 34, 1296, 1459, 1475, 1527
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 7 cut(s) 157, 178, 1204, 1294, 1473, 1525, 1597
PvuII CAGCTG 2 cut(s) 506, 1079
RsaI GTAC 3 cut(s) 34, 306, 741
RsaNI GTAC 3 cut(s) 33, 305, 740
RseI CAYNNNNRTG 1 cut(s) 493
SaqAI TTAA 6 cut(s) 522, 630, 1131, 1557, 1569, 2059
SatI GCNGC 4 cut(s) 929, 1080, 1904, 1923
SchI GAGTC 1 cut(s) 1727
ScrFI CCNGG 7 cut(s) 264, 570, 923, 933, 1292, 1478, 1942
SduI GDGCHC 4 cut(s) 247, 481, 1284, 1462
SfaNI GCATC 5 cut(s) 28, 427, 832, 899, 1909
SmiMI CAYNNNNRTG 1 cut(s) 493
SmlI CTYRAG 4 cut(s) 76, 1130, 1311, 1829
SmoI CTYRAG 4 cut(s) 76, 1130, 1311, 1829
SnaBI TACGTA 1 cut(s) 743
SpeI ACTAGT 1 cut(s) 1852
SphI GCATGC 1 cut(s) 1873
SsiI CCGC 4 cut(s) 215, 728, 980, 1455
SspI AATATT 1 cut(s) 1184
SspMI CTAG 5 cut(s) 428, 1214, 1530, 1655, 1853
StyD4I CCNGG 7 cut(s) 262, 568, 921, 931, 1290, 1476, 1940
StyI CCWWGG 3 cut(s) 682, 1364, 1468
TaaI ACNGT 3 cut(s) 372, 1402, 1967
TaiI ACGT 5 cut(s) 484, 701, 745, 1345, 2035
TaqI TCGA 3 cut(s) 1608, 1911, 2042
TatI WGTACW 1 cut(s) 304
TfiI GAWTC 4 cut(s) 485, 1415, 1433, 1737
Tru1I TTAA 6 cut(s) 522, 630, 1131, 1557, 1569, 2059
Tru9I TTAA 6 cut(s) 522, 630, 1131, 1557, 1569, 2059
TscAI CASTG 5 cut(s) 43, 427, 1291, 1405, 1941
TseI GCWGC 4 cut(s) 928, 1079, 1903, 1922
TspRI CASTG 5 cut(s) 43, 427, 1291, 1405, 1941
Vha464I CTTAAG 1 cut(s) 1130
XagI CCTNNNNNAGG 1 cut(s) 645
XapI RAATTY 5 cut(s) 108, 229, 1018, 1067, 1162
XceI RCATGY 4 cut(s) 95, 681, 1873, 2014
XcmI CCANNNNNNNNNTGG 1 cut(s) 1933
XmiI GTMKAC 1 cut(s) 865
XspI CTAG 5 cut(s) 428, 1214, 1530, 1655, 1853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.