Rh4CG143700

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005499
Physical Location & Seq
Forward (+)
28775517 .. 28790491
14975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG143700.1

Sequence Viewer

Length: 3450 bp
ATGAGAGCGGCGGCGTCTCTTCTTGTCAAATCAGTAGCCTCCTCCAACAATACTTGTTTTCGTTCTCACCACCTCAAATTCTGTCTTGTTGTACTCCAACCACAATCACGGCACTCTTCTTCTTCTTCCTCTTCTCCCATTTCGATTCCGAAGCGCGGCTTTTCCGGCTACGCTGCGGAGCAGTTCTCCGACGACGAGTATGAGTGCGATTTCGAAAGCCAAAAGGCTTCATCTTCCGTGGCCAACATTGACGAGTGGAAATGGAAACTGAGCCTGCTGCTGCGCAGTGACAAGGACCAAGAGATTGTTTCCAGAGATAAAAGGGATAGGAGAGACTATGAGCAAATATCTAACCTCGCCAAACGGATGGGACTTTACACTGAAATATATGGCAAAGTGGTGGTTGCCAGCAAGGTTCCTCTTCCAAACTACCGCCCCGATTTGGATGATAAGCGCCCACAAAGAGAGGTGGTTATCCCTCTTGGCTTGCAAAGGAGGGTAGAGAGTTTACTGCAAGAACACCTTGATCGTGTCCTGCTAAGTTCCCGCAACGTATCAGCCAATTCAGGTGGTGACAGTGAGCACTTCGATCAGGTTGAAAATGATAAGCCTGATGCGAATGCAGATTCTCTTCTTGATGGGTCTGTAATGGAGAAGGTTCTTCAGCGTAGGAGTTTGCGCATGCGTAATATGCAAAGAGCTTGGCAGGAATCACCTGAAGGTAAAAAGATGCTAGAGTTCCGGAAATCACTACCATCTTTCAAAGAGAATCAGAGGTTGCTGCAAGCAATAGCACAAAATCAGGTAGTAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAGCTTCCTCAATATATACTAGAGTCAGAGATAGAATCTGGCCGTGGAGCATTCTGTAGCATTATCTGTACACAACCTCGAAGAATATCTGCTATGGCTGTTGCTGAAAGAGTGTCTGCAGAGAGGGGAGAGCCTCTTGGTGAAACAGTGGGCTATAAAGTTCGATTAGAGGGAATGAAAGGAAAAAATACGCATCTCCTTTTCTGTACCAGTGGTATTCTACTTCGCCGTCTGTTGAGTGATCGGAACTTGAGTGGTGTAACACATGTTTTTGTTGATGAAATTCATGAACGAGGCATGAATGAAGACTTCTTGTTGATTGTATTGAAAGATCTGCTTCCACGCCGTCGGGATCTAAGATTGGTTTTGATGAGTGCCACTCTGAATGCGGAGCTATTTTCAAATTTTTTTGGAGGAGCACCAACAATTCACATTCCGGGTTTCACATATCCAGTGAGAGCACACTTTTTGGAAGATGCACTGGAAATGACTGGGTATAAGCTGACTTCTTTCAACCAAATTGATGACTATGGCCAAGACAAACTGTGGAAAACGCAGAAGCAGTTAGCGCCTCGGAAAAGGAAAAACCAGATCACTGCTCTAGTTGAGGATGCTCTTAGTAAATCGAGTTTTGAGAATTATAGCCCCAGAGCACGCGATTCATTATCATGTTGGACGGCTGATTGCATAGGATTTAATCTTATTGAAGCAGTTTTATGTCATATATGTCGGAAGGAACGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGAGGATATCAGCTGTTTGAGGGATCAACTTAAAGCCCATCCTCTTTTAGGTGATCCTAACAGGGTTTTGCTTATAACATGCCATGGGTCAATGACCACGTCTGAACAGAACCTCATTTTCGCGAGACCGCCTCCTAATGTACGTAAAATTGTTCTTGCTACTAATATGGCAGAAGCAAGCATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCACATATGATGCTTTGAATAATACACCTTGTCTACTACCTTCTTGGATATCCCAAGCATCTGCTCGGCAAAGAAGGGGAAGGGCTGGCCGGGTGCAGCCAGGCGAATGTTACCACCTTTACCCCAAATGTGTGTATCATGCGTTTGCAGAATACCAACTTCCTGAACTCCTAAGGACTCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTTTGCAGGTTGGTAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCACCTGAACCCCTTGCTGTTCATAATGCTGTTGGCTTTCTTAAGTTGATTGGGGCATTGGATGAGAATGAAAATTTGACAAATCTCGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTAAGTGTCCGGGATCCATTCCTTTTGCCTCAAGACAAAAAAGATTTAGCGGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTATAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCAGAAAGAGAAGGATCAGCATATGAATACTGCTGGAGAAATTTTCTCTCTGCCCAGACACTTCAAGCTATTCATTCTCTTCGGAAGCAGTTTAGCTACATCTTAAGAGACGCTGGCTTGGTGGATGCAGATGCAAGCATTAACAACAAGCTAAGTCACAATCAGTCATTGGTCCGCGCAATCATATGTTCCGGACTATTCCCTGGCATAGCATCTGTAGTGCACCGAGAGACATCCATGTCTTTCAAAACTATGGATGATGGCCAGGTTTTACTATATGCTAATTCTGTAAATGCACGTTACCAAACAATTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAGTAAAGGTCAACACAGTTTTCATACGCGATTCTACTGGTGTCTCTGATTCCATTTTGATCCTTTTTGGTGGTGCCCTAAACCATGGTATCCAGGCTGGGCATTTGAAAATGTTGGACGGCTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGCTTAAAGAAGAGCTTAATGAGCTTATTCAGAAGAAGCTTCAAGATCCAAGCCTCGATATCCACAAGGAAGGAAAGTATCTCATACTTGCTGTTCAAGAGCTGGTATCTGGAGATCAAAGTGAAGGAAGATTTGTGTTTGGCCGTGAAAACAGGAAGCCAAAGGAGTCCGGTGATAACAGCAGATTCACAAGGGATGGAACCAATCCTAAGAGTTTGCTACAAACTCTATTGATGAGAGCAGGACATTCACCTCCAAAATACAAAACCAAGCACCTCAAGACAAATGAGTTCAGAGCACTTGTGGAGTTCAAGGGCATGCAATTTGTTGGGAAGCCCAAGAAAAATAAGCAGCTTGCCGAAAGGGATGCTGCCATAGAAGCACTGGCCTGGTTGACTCACACCTCAGACAACAGTCGTGATGAAGATGACAACTCCCCACCTGATGTCACAAACAACATGCTGAAACTTCTGGGGAAACGTAGAAGATCAAAGCGCCACTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1149

Amino Acids

129.19

Weight (kDa)

8.85

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 259 - 413 2.3e-09 DEAD/DEAH box helicase
Helicase_C PF00271 520 - 649 3.6e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 713 - 741 2.8e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 742 - 802 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 869 - 950 5.4e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 959 - 1017 2.6e-23 C-terminal domain in RNA helicases
dsrm PF00035 1050 - 1112 7.7e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1694
AasI GACNNNNNNGTC 1 cut(s) 2671
Acc16I TGCGCA 2 cut(s) 284, 680
Acc36I ACCTGC 1 cut(s) 2059
AccB1I GGYRCC 1 cut(s) 2846
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 1885
AccII CGCG 5 cut(s) 156, 1503, 1742, 2610, 2802
AccIII TCCGGA 2 cut(s) 741, 2624
AcoI YGGCCR 7 cut(s) 240, 886, 1378, 1586, 1939, 2695, 3082
AcsI RAATTY 7 cut(s) 77, 1128, 1249, 2038, 2087, 2182, 2473
AcuI CTGAAG 2 cut(s) 647, 738
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 4 cut(s) 93, 916, 1054, 1761
AfiI CCNNNNNNNGG 7 cut(s) 155, 442, 1667, 2234, 2235, 2754, 2758
AflII CTTAAG 2 cut(s) 2150, 2536
AflIII ACRYGT 1 cut(s) 1111
AjiI CACGTC 1 cut(s) 1719
AjnI CCWGG 6 cut(s) 1588, 1951, 2635, 2697, 2865, 3329
Alw21I GWGCWC 6 cut(s) 585, 1267, 1309, 1501, 2658, 3241
Alw26I GTCTC 8 cut(s) 21, 327, 818, 1738, 1844, 2535, 2657, 2821
Alw44I GTGCAC 1 cut(s) 2654
AlwNI CAGNNNCTG 1 cut(s) 1595
Aor13HI TCCGGA 2 cut(s) 741, 2624
AoxI GGCC 8 cut(s) 240, 886, 1378, 1586, 1939, 2695, 3082, 3327
ApaLI GTGCAC 1 cut(s) 2654
ApeKI GCWGC 8 cut(s) 173, 277, 280, 781, 1948, 2099, 3292, 3311
ApoI RAATTY 7 cut(s) 77, 1128, 1249, 2038, 2087, 2182, 2473
AspLEI GCGC 7 cut(s) 156, 285, 456, 681, 1417, 2612, 3438
AspS9I GGNCC 2 cut(s) 295, 2605
AsuC2I CCSGG 3 cut(s) 1284, 1943, 2312
AsuHPI GGTGA 8 cut(s) 59, 584, 705, 998, 1682, 2780, 3125, 3183
AsuII TTCGAA 1 cut(s) 213
AvaII GGWCC 2 cut(s) 295, 2605
AxyI CCTNAGG 1 cut(s) 2024
BaeGI GKGCMC 2 cut(s) 2658, 2851
BaeI ACNNNNGTAYC 2 cut(s) 2845, 2878
BalI TGGCCA 3 cut(s) 242, 1380, 2697
BamHI GGATCC 2 cut(s) 2314, 2914
BanI GGYRCC 1 cut(s) 2846
BbsI GAAGAC 1 cut(s) 1158
Bbv12I GWGCWC 6 cut(s) 585, 1267, 1309, 1501, 2658, 3241
BbvI GCAGC 8 cut(s) 160, 264, 267, 768, 1960, 2086, 3298, 3304
BccI CCATC 8 cut(s) 361, 632, 763, 1665, 2418, 2687, 2920, 3131
BceAI ACGGC 8 cut(s) 125, 873, 1059, 1176, 1539, 1601, 2908, 3069
BcgI CGANNNNNNTGC 2 cut(s) 3290, 3324
BciT130I CCWGG 6 cut(s) 1590, 1953, 2637, 2699, 2867, 3331
BciVI GTATCC 1 cut(s) 2873
BclI TGATCA 1 cut(s) 2392
BcnI CCSGG 3 cut(s) 1284, 1943, 2312
BcoDI GTCTC 8 cut(s) 21, 327, 818, 1738, 1844, 2535, 2657, 2821
BfaI CTAG 6 cut(s) 734, 866, 1448, 2234, 2367, 2919
BfmI CTRYAG 3 cut(s) 901, 963, 2649
BfoI RGCGCY 3 cut(s) 457, 1418, 3439
BfrI CTTAAG 2 cut(s) 2150, 2536
BfuAI ACCTGC 1 cut(s) 2059
BfuI GTATCC 1 cut(s) 2873
BglII AGATCT 1 cut(s) 1177
Bme1390I CCNGG 9 cut(s) 1284, 1590, 1943, 1953, 2312, 2637, 2699, 2867, 3331
Bme18I GGWCC 2 cut(s) 295, 2605
BmgBI CACGTC 1 cut(s) 1719
BmgT120I GGNCC 2 cut(s) 295, 2605
BmiI GGNNCC 5 cut(s) 417, 2316, 2848, 2916, 3142
BmrFI CCNGG 9 cut(s) 1284, 1590, 1943, 1953, 2312, 2637, 2699, 2867, 3331
BmrI ACTGGG 2 cut(s) 1347, 1623
BmuI ACTGGG 2 cut(s) 1347, 1623
BoxI GACNNNNGTC 1 cut(s) 3354
BpiI GAAGAC 1 cut(s) 1158
BplI GAGNNNNNCTC 6 cut(s) 170, 202, 1210, 1242, 1735, 1767
BpmI CTGGAG 2 cut(s) 2488, 3072
Bpu14I TTCGAA 1 cut(s) 213
BpuEI CTTGAG 3 cut(s) 1117, 2316, 3203
BpuMI CCSGG 3 cut(s) 1284, 1943, 2312
BsaAI YACGTR 1 cut(s) 1763
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 2 cut(s) 1738, 1844
BsaJI CCNNGG 7 cut(s) 237, 889, 1418, 1702, 2635, 2753, 2857
BsaWI WCCGGW 3 cut(s) 741, 2624, 3110
Bsc4I CCNNNNNNNGG 7 cut(s) 155, 442, 1667, 2234, 2235, 2754, 2758
Bse1I ACTGG 9 cut(s) 832, 1056, 1298, 1332, 1342, 1618, 2219, 2815, 3330
Bse21I CCTNAGG 1 cut(s) 2024
Bse3DI GCAATG 1 cut(s) 2266
BseAI TCCGGA 2 cut(s) 741, 2624
BseBI CCWGG 6 cut(s) 1590, 1953, 2637, 2699, 2867, 3331
BseDI CCNNGG 7 cut(s) 237, 889, 1418, 1702, 2635, 2753, 2857
BseLI CCNNNNNNNGG 7 cut(s) 155, 442, 1667, 2234, 2235, 2754, 2758
BseMI GCAATG 1 cut(s) 2266
BseMII CTCAG 3 cut(s) 260, 860, 3360
BseNI ACTGG 9 cut(s) 832, 1056, 1298, 1332, 1342, 1618, 2219, 2815, 3330
BseRI GAGGAG 2 cut(s) 31, 1275
BseSI GKGCMC 2 cut(s) 2658, 2851
BseXI GCAGC 8 cut(s) 160, 264, 267, 768, 1960, 2086, 3298, 3304
BseYI CCCAGC 1 cut(s) 2870
BsgI GTGCAG 1 cut(s) 1967
Bsh1236I CGCG 5 cut(s) 156, 1503, 1742, 2610, 2802
BshFI GGCC 8 cut(s) 242, 888, 1380, 1588, 1941, 2697, 3084, 3329
BshNI GGYRCC 1 cut(s) 2846
BsiHKAI GWGCWC 6 cut(s) 585, 1267, 1309, 1501, 2658, 3241
BsiSI CCGG 7 cut(s) 165, 742, 1283, 1942, 2311, 2625, 3111
BslFI GGGAC 2 cut(s) 384, 2368
BslI CCNNNNNNNGG 7 cut(s) 155, 442, 1667, 2234, 2235, 2754, 2758
BsmAI GTCTC 8 cut(s) 21, 327, 818, 1738, 1844, 2535, 2657, 2821
BsmBI CGTCTC 2 cut(s) 21, 2535
BsmFI GGGAC 2 cut(s) 384, 2368
BsmI GAATGC 3 cut(s) 625, 896, 1237
BsnI GGCC 8 cut(s) 242, 888, 1380, 1588, 1941, 2697, 3084, 3329
Bso31I GGTCTC 2 cut(s) 1738, 1844
Bsp119I TTCGAA 1 cut(s) 213
Bsp1286I GDGCHC 7 cut(s) 585, 1267, 1309, 1501, 2658, 2851, 3241
Bsp13I TCCGGA 2 cut(s) 741, 2624
Bsp1407I TGTACA 1 cut(s) 914
Bsp19I CCATGG 2 cut(s) 1702, 2857
Bsp68I TCGCGA 1 cut(s) 1742
BspANI GGCC 8 cut(s) 242, 888, 1380, 1588, 1941, 2697, 3084, 3329
BspCNI CTCAG 3 cut(s) 261, 859, 3359
BspEI TCCGGA 2 cut(s) 741, 2624
BspFNI CGCG 5 cut(s) 156, 1503, 1742, 2610, 2802
BspHI TCATGA 2 cut(s) 1132, 1608
BspLI GGNNCC 5 cut(s) 417, 2316, 2848, 2916, 3142
BspMAI CTGCAG 1 cut(s) 967
BspMI ACCTGC 1 cut(s) 2059
BspQI GCTCTTC 1 cut(s) 2946
BspT104I TTCGAA 1 cut(s) 213
BspT107I GGYRCC 1 cut(s) 2846
BspTI CTTAAG 2 cut(s) 2150, 2536
BspTNI GGTCTC 2 cut(s) 1738, 1844
BsrBI CCGCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 2266
BsrGI TGTACA 1 cut(s) 914
BsrI ACTGG 9 cut(s) 832, 1056, 1298, 1332, 1342, 1618, 2219, 2815, 3330
BssECI CCNNGG 7 cut(s) 237, 889, 1418, 1702, 2635, 2753, 2857
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 3 cut(s) 1702, 2753, 2857
Bst2UI CCWGG 6 cut(s) 1590, 1953, 2637, 2699, 2867, 3331
Bst4CI ACNGT 5 cut(s) 578, 994, 1392, 2791, 3356
Bst6I CTCTTC 7 cut(s) 24, 121, 136, 426, 636, 2517, 2946
BstACI GRCGYC 1 cut(s) 14
BstAFI CTTAAG 2 cut(s) 2150, 2536
BstAUI TGTACA 1 cut(s) 914
BstBAI YACGTR 1 cut(s) 1763
BstBI TTCGAA 1 cut(s) 213
BstDSI CCRYGG 4 cut(s) 237, 889, 1702, 2857
BstENI CCTNNNNNAGG 1 cut(s) 1665
BstFNI CGCG 5 cut(s) 156, 1503, 1742, 2610, 2802
BstH2I RGCGCY 3 cut(s) 457, 1418, 3439
BstHHI GCGC 7 cut(s) 156, 285, 456, 681, 1417, 2612, 3438
BstMAI GTCTC 8 cut(s) 21, 327, 818, 1738, 1844, 2535, 2657, 2821
BstMWI GCNNNNNNNGC 6 cut(s) 165, 691, 1414, 2357, 2962, 3320
BstNI CCWGG 6 cut(s) 1590, 1953, 2637, 2699, 2867, 3331
BstNSI RCATGY 5 cut(s) 685, 1115, 1701, 3262, 3403
BstPAI GACNNNNGTC 1 cut(s) 3354
BstSCI CCNGG 9 cut(s) 1282, 1588, 1941, 1951, 2310, 2635, 2697, 2865, 3329
BstSFI CTRYAG 3 cut(s) 901, 963, 2649
BstSLI GKGCMC 2 cut(s) 2658, 2851
BstSNI TACGTA 1 cut(s) 1763
BstUI CGCG 5 cut(s) 156, 1503, 1742, 2610, 2802
BstV1I GCAGC 8 cut(s) 160, 264, 267, 768, 1960, 2086, 3298, 3304
BstV2I GAAGAC 1 cut(s) 1158
BstX2I RGATCY 6 cut(s) 1177, 1198, 2224, 2314, 2914, 2986
BstXI CCANNNNNNTGG 1 cut(s) 367
BstYI RGATCY 6 cut(s) 1177, 1198, 2224, 2314, 2914, 2986
Bsu36I CCTNAGG 1 cut(s) 2024
BsuI GTATCC 1 cut(s) 2873
BsuRI GGCC 8 cut(s) 242, 888, 1380, 1588, 1941, 2697, 3084, 3329
BtgI CCRYGG 4 cut(s) 237, 889, 1702, 2857
BtrI CACGTC 1 cut(s) 1719
BtsI GCAGTG 2 cut(s) 292, 1440
BtsIMutI CAGTG 9 cut(s) 292, 378, 583, 999, 1063, 1305, 1325, 1440, 3323
BtuMI TCGCGA 1 cut(s) 1742
BveI ACCTGC 1 cut(s) 2059
CaiI CAGNNNCTG 1 cut(s) 1595
CciI TCATGA 2 cut(s) 1132, 1608
CfoI GCGC 7 cut(s) 156, 285, 456, 681, 1417, 2612, 3438
Cfr13I GGNCC 2 cut(s) 295, 2605
CseI GACGC 2 cut(s) 3, 2552
Csp6I GTAC 4 cut(s) 92, 915, 1053, 1760
CspCI CAANNNNNGTGG 2 cut(s) 550, 585
CviQI GTAC 4 cut(s) 92, 915, 1053, 1760
DrdI GACNNNNNNGTC 1 cut(s) 2671
DseDI GACNNNNNNGTC 1 cut(s) 2671
EaeI YGGCCR 7 cut(s) 240, 886, 1378, 1586, 1939, 2695, 3082
Eam1104I CTCTTC 7 cut(s) 24, 121, 136, 426, 636, 2517, 2946
EarI CTCTTC 7 cut(s) 24, 121, 136, 426, 636, 2517, 2946
Eco105I TACGTA 1 cut(s) 1763
Eco130I CCWWGG 3 cut(s) 1702, 2753, 2857
Eco31I GGTCTC 2 cut(s) 1738, 1844
Eco32I GATATC 4 cut(s) 816, 1627, 1902, 3001
Eco47I GGWCC 2 cut(s) 295, 2605
Eco57I CTGAAG 2 cut(s) 647, 738
Eco81I CCTNAGG 1 cut(s) 2024
EcoNI CCTNNNNNAGG 1 cut(s) 1665
EcoRI GAATTC 2 cut(s) 2038, 2087
EcoRII CCWGG 6 cut(s) 1588, 1951, 2635, 2697, 2865, 3329
EcoRV GATATC 4 cut(s) 816, 1627, 1902, 3001
EcoT14I CCWWGG 3 cut(s) 1702, 2753, 2857
ErhI CCWWGG 3 cut(s) 1702, 2753, 2857
Esp3I CGTCTC 2 cut(s) 21, 2535
FaqI GGGAC 2 cut(s) 384, 2368
FauI CCCGC 2 cut(s) 554, 2344
FauNDI CATATG 5 cut(s) 1858, 2397, 2416, 2455, 2618
FbaI TGATCA 1 cut(s) 2392
FblI GTMKAC 1 cut(s) 1885
FspBI CTAG 6 cut(s) 734, 866, 1448, 2234, 2367, 2919
FspI TGCGCA 2 cut(s) 284, 680
GlaI GCGC 7 cut(s) 155, 284, 455, 680, 1416, 2611, 3437
GsaI CCCAGC 1 cut(s) 2874
GsuI CTGGAG 2 cut(s) 2488, 3072
HaeII RGCGCY 3 cut(s) 457, 1418, 3439
HaeIII GGCC 8 cut(s) 242, 888, 1380, 1588, 1941, 2697, 3084, 3329
HapII CCGG 7 cut(s) 165, 742, 1283, 1942, 2311, 2625, 3111
HgaI GACGC 2 cut(s) 3, 2552
HhaI GCGC 7 cut(s) 156, 285, 456, 681, 1417, 2612, 3438
Hin1I GRCGYC 1 cut(s) 14
Hin6I GCGC 7 cut(s) 154, 283, 454, 679, 1415, 2610, 3436
HinP1I GCGC 7 cut(s) 154, 283, 454, 679, 1415, 2610, 3436
HincII GTYRAC 2 cut(s) 2785, 3336
HindII GTYRAC 2 cut(s) 2785, 3336
HindIII AAGCTT 2 cut(s) 2942, 2978
HpaII CCGG 7 cut(s) 165, 742, 1283, 1942, 2311, 2625, 3111
HphI GGTGA 8 cut(s) 59, 584, 705, 998, 1682, 2780, 3125, 3183
Hpy166II GTNNAC 6 cut(s) 509, 917, 1886, 2656, 2785, 3336
Hpy8I GTNNAC 6 cut(s) 509, 917, 1886, 2656, 2785, 3336
Hpy99I CGWCG 3 cut(s) 194, 197, 1197
HpyCH4III ACNGT 5 cut(s) 578, 994, 1392, 2791, 3356
HpyCH4IV ACGT 5 cut(s) 552, 1718, 1762, 2731, 3421
HpyF10VI GCNNNNNNNGC 6 cut(s) 165, 691, 1414, 2357, 2962, 3320
HpySE526I ACGT 5 cut(s) 552, 1718, 1762, 2731, 3421
Hsp92I GRCGYC 1 cut(s) 14
HspAI GCGC 7 cut(s) 154, 283, 454, 679, 1415, 2610, 3436
Kpn2I TCCGGA 2 cut(s) 741, 2624
Ksp22I TGATCA 1 cut(s) 2392
LguI GCTCTTC 1 cut(s) 2946
LmnI GCTCC 4 cut(s) 178, 893, 1237, 1262
Lsp1109I GCAGC 8 cut(s) 160, 264, 267, 768, 1960, 2086, 3298, 3304
MaeI CTAG 6 cut(s) 734, 866, 1448, 2234, 2367, 2919
MaeII ACGT 5 cut(s) 552, 1718, 1762, 2731, 3421
MaeIII GTNAC 7 cut(s) 287, 572, 1105, 1961, 2588, 2732, 3388
MbiI CCGCTC 1 cut(s) 8
MflI RGATCY 6 cut(s) 1177, 1198, 2224, 2314, 2914, 2986
MhlI GDGCHC 7 cut(s) 585, 1267, 1309, 1501, 2658, 2851, 3241
MlsI TGGCCA 3 cut(s) 242, 1380, 2697
MluNI TGGCCA 3 cut(s) 242, 1380, 2697
MlyI GAGTC 4 cut(s) 878, 2023, 3116, 3331
MmeI TCCRAC 6 cut(s) 69, 121, 213, 1499, 1556, 2868
Mox20I TGGCCA 3 cut(s) 242, 1380, 2697
MroI TCCGGA 2 cut(s) 741, 2624
MscI TGGCCA 3 cut(s) 242, 1380, 2697
MseI TTAA 8 cut(s) 1542, 1650, 2151, 2537, 2574, 2946, 2958, 3448
MslI CAYNNNNRTG 1 cut(s) 1513
Msp20I TGGCCA 3 cut(s) 242, 1380, 2697
MspA1I CMGCKG 2 cut(s) 1632, 2099
MspCI CTTAAG 2 cut(s) 2150, 2536
MspI CCGG 7 cut(s) 165, 742, 1283, 1942, 2311, 2625, 3111
MspR9I CCNGG 9 cut(s) 1284, 1590, 1943, 1953, 2312, 2637, 2699, 2867, 3331
Mva1269I GAATGC 3 cut(s) 625, 896, 1237
MvaI CCWGG 6 cut(s) 1590, 1953, 2637, 2699, 2867, 3331
MvnI CGCG 5 cut(s) 156, 1503, 1742, 2610, 2802
MwoI GCNNNNNNNGC 6 cut(s) 165, 691, 1414, 2357, 2962, 3320
NciI CCSGG 3 cut(s) 1284, 1943, 2312
NcoI CCATGG 2 cut(s) 1702, 2857
NdeI CATATG 5 cut(s) 1858, 2397, 2416, 2455, 2618
NlaIV GGNNCC 5 cut(s) 417, 2316, 2848, 2916, 3142
NmeAIII GCCGAG 1 cut(s) 1897
NmuCI GTSAC 4 cut(s) 287, 572, 2588, 3388
NruI TCGCGA 1 cut(s) 1742
NsbI TGCGCA 2 cut(s) 284, 680
NspI RCATGY 5 cut(s) 685, 1115, 1701, 3262, 3403
NspV TTCGAA 1 cut(s) 213
PaeI GCATGC 2 cut(s) 685, 3262
PagI TCATGA 2 cut(s) 1132, 1608
PciI ACATGT 1 cut(s) 1111
PciSI GCTCTTC 1 cut(s) 2946
PctI GAATGC 3 cut(s) 625, 896, 1237
PflFI GACNNNGTC 1 cut(s) 1717
PfoI TCCNGGA 1 cut(s) 2310
PleI GAGTC 4 cut(s) 877, 2023, 3115, 3331
PpsI GAGTC 4 cut(s) 877, 2023, 3115, 3331
Ppu21I YACGTR 1 cut(s) 1763
PscI ACATGT 1 cut(s) 1111
PshAI GACNNNNGTC 1 cut(s) 3354
PsiI TTATAA 1 cut(s) 1694
Psp6I CCWGG 6 cut(s) 1588, 1951, 2635, 2697, 2865, 3329
PspFI CCCAGC 1 cut(s) 2870
PspGI CCWGG 6 cut(s) 1588, 1951, 2635, 2697, 2865, 3329
PspN4I GGNNCC 5 cut(s) 417, 2316, 2848, 2916, 3142
PspPI GGNCC 2 cut(s) 295, 2605
PstI CTGCAG 1 cut(s) 967
PstNI CAGNNNCTG 1 cut(s) 1595
PsuI RGATCY 6 cut(s) 1177, 1198, 2224, 2314, 2914, 2986
PsyI GACNNNGTC 1 cut(s) 1717
PvuII CAGCTG 2 cut(s) 1632, 2099
RruI TCGCGA 1 cut(s) 1742
RsaI GTAC 4 cut(s) 93, 916, 1054, 1761
RsaNI GTAC 4 cut(s) 92, 915, 1053, 1760
RseI CAYNNNNRTG 1 cut(s) 1513
SapI GCTCTTC 1 cut(s) 2946
SaqAI TTAA 8 cut(s) 1542, 1650, 2151, 2537, 2574, 2946, 2958, 3448
Sau96I GGNCC 2 cut(s) 295, 2605
SchI GAGTC 4 cut(s) 878, 2023, 3116, 3331
ScrFI CCNGG 9 cut(s) 1284, 1590, 1943, 1953, 2312, 2637, 2699, 2867, 3331
SduI GDGCHC 7 cut(s) 585, 1267, 1309, 1501, 2658, 2851, 3241
SfcI CTRYAG 3 cut(s) 901, 963, 2649
SfuI TTCGAA 1 cut(s) 213
SinI GGWCC 2 cut(s) 295, 2605
SmiMI CAYNNNNRTG 1 cut(s) 1513
SmlI CTYRAG 5 cut(s) 1096, 2150, 2331, 2536, 3218
SmoI CTYRAG 5 cut(s) 1096, 2150, 2331, 2536, 3218
SnaBI TACGTA 1 cut(s) 1763
SphI GCATGC 2 cut(s) 685, 3262
SspI AATATT 1 cut(s) 2204
SspMI CTAG 6 cut(s) 734, 866, 1448, 2234, 2367, 2919
StyD4I CCNGG 9 cut(s) 1282, 1588, 1941, 1951, 2310, 2635, 2697, 2865, 3329
StyI CCWWGG 3 cut(s) 1702, 2753, 2857
TaaI ACNGT 5 cut(s) 578, 994, 1392, 2791, 3356
TaiI ACGT 5 cut(s) 555, 1721, 1765, 2734, 3424
TaqI TCGA 7 cut(s) 143, 213, 588, 925, 1009, 1472, 2997
TatI WGTACW 2 cut(s) 91, 914
TauI GCSGC 3 cut(s) 11, 14, 159
Tru1I TTAA 8 cut(s) 1542, 1650, 2151, 2537, 2574, 2946, 2958, 3448
Tru9I TTAA 8 cut(s) 1542, 1650, 2151, 2537, 2574, 2946, 2958, 3448
TscAI CASTG 9 cut(s) 292, 385, 583, 999, 1063, 1305, 1332, 1447, 3330
TseFI GTSAC 4 cut(s) 287, 572, 2588, 3388
TseI GCWGC 8 cut(s) 173, 277, 280, 781, 1948, 2099, 3292, 3311
Tsp45I GTSAC 4 cut(s) 287, 572, 2588, 3388
TspGWI ACGGA 2 cut(s) 226, 379
TspRI CASTG 9 cut(s) 292, 385, 583, 999, 1063, 1305, 1332, 1447, 3330
Tth111I GACNNNGTC 1 cut(s) 1717
Vha464I CTTAAG 2 cut(s) 2150, 2536
VneI GTGCAC 1 cut(s) 2654
VpaK11BI GGWCC 2 cut(s) 295, 2605
XagI CCTNNNNNAGG 1 cut(s) 1665
XapI RAATTY 7 cut(s) 77, 1128, 1249, 2038, 2087, 2182, 2473
XbaI TCTAGA 1 cut(s) 2366
XceI RCATGY 5 cut(s) 685, 1115, 1701, 3262, 3403
XcmI CCANNNNNNNNNTGG 1 cut(s) 3322
XmiI GTMKAC 1 cut(s) 1885
XspI CTAG 6 cut(s) 734, 866, 1448, 2234, 2367, 2919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.