FvH4_5g28161

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005499
Physical Location & Seq
Reverse (-)
19358653 .. 19372749
14097 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28161.t4

Sequence Viewer

Length: 3381 bp
ATGCATTATTCGAGAGCGGCGGTGTCTCTGCTTCTCAAATCAGTAGCCTCCTCCTCCAACCATTCTTCTTGTTTTCGTTCTCACCACCTCACCCTCTGTCTCTCTAACCGCCGCTACACTGCGGAGCAGTTCTCCGACGACGAGTATGAGTGCGATTTCGAAAGCCAAAAGGCTTCATCTTCAGTGGCCAACATTGACGAGTGGAAATGGAAACTGAGCCTGCTGCTGCGTAGTGACAAGGACCAAGAAATTGTTTCAAGAGATAAAAGAGATAGGAGAGACTATGAGCAGATATCTAACCTCGCCAAAAGGATGGGACTTCACAGTGAAATATATGGCAAAGTGGTGGTGGCCAGCAAGGTTCCTCTTCCAAACTACCGCCCCGATTTGGATGATAAGCGCCCACAAAGAGAGGTGGTTATTCCTCTTGGCTTGCAAAGGAGGGTAGAGGGTCTACTGCAAGAACACCTTGATTGTCTCCTGCTGAGTTCCTGGAAGGTACCAGCCAATTTAGGTGACAGTGAGTACCTTGATCAGGTTGATAAGGAGAATGCGGATTCTTATCTTGATGGTTCTGTAATGGAGAAGGTACTTCAGCGTAGGAGTTTGTGGATGCGTAATTTGCAAAGAGCTTGGCAGGAATCACCTGAAGGAAAAAAGATGCTAGAATTCAGGAAATCACTACCATCGTTCAAGGAGAATCAGAGGTTGCTGCAAGCAATTGCACAAAATCAGGTTGTAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAGCTTCCTCAATATATACTAGAGTCAGAGATAGAATCTGGCCGTGGAGCATTCTGTAACATTATCTGTACACAACCTCGAAGAATATCTGCCATGTCTGTTGCTGAAAGAGTGTCTGCAGAGAGGGGAGAGTCTCTTGGTGAAACAGTGGGTTATAAAGTTCGATTAGAGGGAATGAAAGGAAAAAATACCCATCTTCTTTTCTGTACCAGTGGCATTTTACTTCGCCGTCTGTTGAGTGATCGGAACTTGAGTGGTATCACACATGTTTTTGTTGATGAAATTCATGAACGAGGCATGAATGAAGACTTCTTGTTGATTGTATTGAAAGATCTGCTTCCACGCCGTCGCGATATAAGATTAATTTTGATGAGTGCCACTCTGAATGCGGAGCTATTTTCAAAATTCTTTGGAGGAGCGCCAACAATTCACATTCCGGGTTTCACATATCCAGTGAGAGCACACTTTTTGGAAGATGTACTGGAAATGACTGGGTATAAGCTGACTTTTTTCAATCAAATTGATGACTATGGCCAAGACAAACTGTGGAAAATGCAGAAGCAGTTAGCGCCTCGGAAAAGGAAAAACCAGATCACTGCTCTAGTTGAGGATGCTCTTAGTAAATCAAGCTTTGAGAATTATAGCTCCAGAGCACGTGATTCATTATCATGTTGGACAGCTGATTGCATAGGATTTAATCTGATTGAAGCAGTTTTATGTCATATATGTCGGAAGGAGCGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGATGATATCAGCTGTTTGAAGGATCAACTTAAAGCGCATCCTCTTTTAGGTGATCCGAACAGGGTTTTGCTTATAACCTGCCATGGGTCAATGGCCACGTCTGAACAGAACCTCATTTTTGGGAGACCACCTCCTAATGTACGTAAAATAGTTCTTGCTACTAATATGGCAGAAGCAAGCATTACAATCAATGACGTGGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCACATATGATGCTTTGAATAATACACCTTGTTTACTACCTTCTTGGATATCCCAAGCATCTGCTCGGCAAAGAAGGGGAAGGGCTGGCCGGGTGCAGCCAGGTGAGTGTTACCACCTTTATCCCAAATGTGTATATCATGCTTTTGCGGAATACCAACTTCCTGAACTTCTAAGGACTCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTTTGCAGGTTGATAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCACCTGAACCACTTGCTGTTCAAAATGCTATTGACTTTCTTAAGTTGATTGGGGCATTGGATGAGAATGAAAATTTGACAAATCTCGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCCGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTAAGTGTTCGGGATCCATTCCTTTTGCCTCAAGACAAAAAAGATTTAGCAGGTACAGCAAAATCTAGATTCTCTGCAAAAGATTACAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCAGAAAGAGAAGGATCAGCGTATGAATACTGCTGGAGAAATTTTCTCTCTGCCCAAACACTTCAAGCTATTCATTCTCTCCGGAAGCAGTTTAGCTACATCTTAAGAGACGCTGGCTTGGTGGATGCGGATGCAAGCATTAACAACAAGCTAAGTCACAATCAGTCATTGGTCCGTGCAATCATATGTTCTGGACTCTTCCCTGGCATAGCATCTGTAGTGCACCGAGAGACATCCCTGTCTTTCAAAACTATGGATGATGGCCAGGTTTTACTATATGCTAATTCTGTAAATGCACGTTACCAAACGATTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAATAAAAGTCAACACTGTTTTCATACGCGATTCTACTGGTGTTTCTGATTCCATTTTGATCCTTTTTGGTGGTGCCCTAACCCATGGGATCCAGGCTGGGCATCTGAAAATGTTGGATGGTTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGATTAAAGAAGAGCTTAATGAGCTTATTCAAAAGAAGCTTCAAGATCCAAGCCTGGATATCCACAAGGAAGGAAAGTATCTCATACTTGCTGTTCAAGAGCTGGTATCTGGAGATCAAAGTGAAGGAAGATTTGTGTTTGGCCGTGAAAACAGGAAGCCAAGGGAGTCTGGTGATAACAGCAGATTCACAAGAGACGGAACCAACCCTAAGAGTTTGCTGCAAACTTTATTGATGAGAGCGGGACATTCAGCTCCAAAATATAAAACAAAGCACCTCAAGACAAATGAGTTCAGAGCCCTTGTGGAATTCAAGGGCATGCAGTTTGTTGGAAAGCCCAAGAAAAATAAGCAGCTCGCGGAAAGGGATGCTGCCGTAGAAGCACTGGCCTGGTTGACTCACACCTCAGACGACAGTCGTGATGAAGATGACAACTCCCCACCTGATGTCACTAATAACATGCTGAAACTTCTGGGGAAACGTAGAAGATCAAAGCGCCACTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1127

Amino Acids

127.25

Weight (kDa)

8.62

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 40 - 169 1.8e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 233 - 261 5.5e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 262 - 322 3e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 389 - 470 3.6e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 479 - 537 6e-23 C-terminal domain in RNA helicases
dsrm PF00035 570 - 632 1.5e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 933, 1625
AasI GACNNNNNNGTC 1 cut(s) 2602
Acc36I ACCTGC 3 cut(s) 1637, 1990, 2273
Acc65I GGTACC 1 cut(s) 499
AccB1I GGYRCC 3 cut(s) 499, 2186, 2777
AccBSI CCGCTC 3 cut(s) 17, 1516, 3113
AccI GTMKAC 1 cut(s) 454
AccII CGCG 3 cut(s) 1128, 2733, 3230
AccIII TCCGGA 1 cut(s) 2445
AcoI YGGCCR 9 cut(s) 186, 351, 817, 1309, 1517, 1644, 1870, 2626, 3013
AcsI RAATTY 8 cut(s) 668, 1059, 1181, 1969, 2018, 2113, 2404, 3179
AcuI CTGAAG 3 cut(s) 165, 578, 669
AcvI CACGTG 1 cut(s) 1433
AfaI GTAC 8 cut(s) 501, 527, 591, 847, 985, 1257, 1692, 2287
AfiI CCNNNNNNNGG 8 cut(s) 388, 535, 1598, 1635, 2165, 2166, 2685, 2689
AflII CTTAAG 2 cut(s) 2081, 2467
AflIII ACRYGT 1 cut(s) 1042
AjiI CACGTC 2 cut(s) 1650, 1747
AjnI CCWGG 8 cut(s) 491, 1519, 1882, 2566, 2628, 2796, 2925, 3260
Alw21I GWGCWC 3 cut(s) 1240, 1432, 2589
Alw44I GTGCAC 1 cut(s) 2585
AlwNI CAGNNNCTG 1 cut(s) 1526
Aor13HI TCCGGA 1 cut(s) 2445
ApaLI GTGCAC 1 cut(s) 2585
ApeKI GCWGC 8 cut(s) 223, 226, 712, 1879, 2030, 3091, 3223, 3242
ApoI RAATTY 8 cut(s) 668, 1059, 1181, 1969, 2018, 2113, 2404, 3179
ArsI GACNNNNNNTTYG 2 cut(s) 1736, 1768
AseI ATTAAT 1 cut(s) 1139
Asp718I GGTACC 1 cut(s) 499
AspLEI GCGC 5 cut(s) 402, 1198, 1348, 1588, 3369
AspS9I GGNCC 2 cut(s) 241, 2536
AsuC2I CCSGG 2 cut(s) 1215, 1874
AsuHPI GGTGA 9 cut(s) 74, 82, 527, 636, 929, 1613, 1898, 2711, 3056
AsuII TTCGAA 1 cut(s) 159
AvaII GGWCC 2 cut(s) 241, 2536
BaeGI GKGCMC 2 cut(s) 2589, 2782
BalI TGGCCA 5 cut(s) 188, 353, 1311, 1646, 2628
BamHI GGATCC 3 cut(s) 2245, 2793, 2845
BanI GGYRCC 3 cut(s) 499, 2186, 2777
BanII GRGCYC 1 cut(s) 3172
BbrPI CACGTG 1 cut(s) 1433
BbsI GAAGAC 1 cut(s) 1089
Bbv12I GWGCWC 3 cut(s) 1240, 1432, 2589
BbvI GCAGC 8 cut(s) 210, 213, 699, 1891, 2017, 3078, 3229, 3235
BccI CCATC 8 cut(s) 307, 563, 694, 978, 2349, 2618, 2816, 2851
BceAI ACGGC 6 cut(s) 804, 990, 1107, 2174, 3000, 3230
BciT130I CCWGG 8 cut(s) 493, 1521, 1884, 2568, 2630, 2798, 2927, 3262
BclI TGATCA 2 cut(s) 532, 2323
BcnI CCSGG 2 cut(s) 1215, 1874
BfaI CTAG 6 cut(s) 665, 797, 1379, 2165, 2298, 2850
BfmI CTRYAG 2 cut(s) 894, 2580
BfoI RGCGCY 4 cut(s) 403, 1199, 1349, 3370
BfrI CTTAAG 2 cut(s) 2081, 2467
BfuAI ACCTGC 3 cut(s) 1637, 1990, 2273
BglII AGATCT 1 cut(s) 1108
Bme18I GGWCC 2 cut(s) 241, 2536
BmgBI CACGTC 2 cut(s) 1650, 1747
BmgT120I GGNCC 2 cut(s) 241, 2536
BmiI GGNNCC 8 cut(s) 363, 501, 2188, 2247, 2779, 2795, 2847, 3073
BmrI ACTGGG 2 cut(s) 1278, 1554
BmuI ACTGGG 2 cut(s) 1278, 1554
BoxI GACNNNNGTC 1 cut(s) 3285
BpiI GAAGAC 1 cut(s) 1089
BplI GAGNNNNNCTC 6 cut(s) 116, 148, 1141, 1173, 1666, 1698
BpmI CTGGAG 3 cut(s) 1408, 2419, 3003
Bpu14I TTCGAA 1 cut(s) 159
BpuEI CTTGAG 3 cut(s) 1048, 2247, 3134
BpuMI CCSGG 2 cut(s) 1215, 1874
BsaAI YACGTR 2 cut(s) 1433, 1694
BsaBI GATNNNNATC 1 cut(s) 561
BsaI GGTCTC 2 cut(s) 1669, 1775
BsaJI CCNNGG 7 cut(s) 820, 1349, 1633, 2566, 2684, 2788, 3032
BsaWI WCCGGW 1 cut(s) 2445
Bsc4I CCNNNNNNNGG 8 cut(s) 388, 535, 1598, 1635, 2165, 2166, 2685, 2689
Bse1I ACTGG 9 cut(s) 763, 987, 1229, 1263, 1273, 1549, 2150, 2746, 3261
Bse3DI GCAATG 1 cut(s) 2197
Bse8I GATNNNNATC 1 cut(s) 561
BseAI TCCGGA 1 cut(s) 2445
BseBI CCWGG 8 cut(s) 493, 1521, 1884, 2568, 2630, 2798, 2927, 3262
BseDI CCNNGG 7 cut(s) 820, 1349, 1633, 2566, 2684, 2788, 3032
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 8 cut(s) 388, 535, 1598, 1635, 2165, 2166, 2685, 2689
BseMI GCAATG 1 cut(s) 2197
BseMII CTCAG 4 cut(s) 206, 476, 791, 3291
BseNI ACTGG 9 cut(s) 763, 987, 1229, 1263, 1273, 1549, 2150, 2746, 3261
BseRI GAGGAG 3 cut(s) 40, 43, 1206
BseSI GKGCMC 2 cut(s) 2589, 2782
BseXI GCAGC 8 cut(s) 210, 213, 699, 1891, 2017, 3078, 3229, 3235
BseYI CCCAGC 1 cut(s) 2801
BsgI GTGCAG 1 cut(s) 1898
Bsh1236I CGCG 3 cut(s) 1128, 2733, 3230
BshNI GGYRCC 3 cut(s) 499, 2186, 2777
BsiHKAI GWGCWC 3 cut(s) 1240, 1432, 2589
BsiSI CCGG 3 cut(s) 1214, 1873, 2446
BslFI GGGAC 2 cut(s) 330, 3129
BslI CCNNNNNNNGG 8 cut(s) 388, 535, 1598, 1635, 2165, 2166, 2685, 2689
BsmBI CGTCTC 2 cut(s) 2466, 3060
BsmFI GGGAC 2 cut(s) 330, 3129
BsmI GAATGC 3 cut(s) 556, 827, 1168
Bso31I GGTCTC 2 cut(s) 1669, 1775
Bsp119I TTCGAA 1 cut(s) 159
Bsp1286I GDGCHC 5 cut(s) 1240, 1432, 2589, 2782, 3172
Bsp13I TCCGGA 1 cut(s) 2445
Bsp1407I TGTACA 1 cut(s) 845
Bsp19I CCATGG 2 cut(s) 1633, 2788
Bsp68I TCGCGA 1 cut(s) 1128
BspCNI CTCAG 4 cut(s) 207, 477, 790, 3290
BspEI TCCGGA 1 cut(s) 2445
BspFNI CGCG 3 cut(s) 1128, 2733, 3230
BspHI TCATGA 2 cut(s) 1063, 1539
BspLI GGNNCC 8 cut(s) 363, 501, 2188, 2247, 2779, 2795, 2847, 3073
BspMAI CTGCAG 1 cut(s) 898
BspMI ACCTGC 3 cut(s) 1637, 1990, 2273
BspQI GCTCTTC 1 cut(s) 2877
BspT104I TTCGAA 1 cut(s) 159
BspT107I GGYRCC 3 cut(s) 499, 2186, 2777
BspTI CTTAAG 2 cut(s) 2081, 2467
BspTNI GGTCTC 2 cut(s) 1669, 1775
BsrBI CCGCTC 3 cut(s) 17, 1516, 3113
BsrDI GCAATG 1 cut(s) 2197
BsrGI TGTACA 1 cut(s) 845
BsrI ACTGG 9 cut(s) 763, 987, 1229, 1263, 1273, 1549, 2150, 2746, 3261
BssECI CCNNGG 7 cut(s) 820, 1349, 1633, 2566, 2684, 2788, 3032
BssT1I CCWWGG 4 cut(s) 1633, 2684, 2788, 3032
Bst2UI CCWGG 8 cut(s) 493, 1521, 1884, 2568, 2630, 2798, 2927, 3262
Bst4CI ACNGT 7 cut(s) 326, 521, 925, 1323, 2321, 2722, 3287
Bst6I CTCTTC 3 cut(s) 372, 2567, 2877
BstAFI CTTAAG 2 cut(s) 2081, 2467
BstAUI TGTACA 1 cut(s) 845
BstBAI YACGTR 2 cut(s) 1433, 1694
BstBI TTCGAA 1 cut(s) 159
BstDSI CCRYGG 3 cut(s) 820, 1633, 2788
BstENI CCTNNNNNAGG 2 cut(s) 533, 1596
BstFNI CGCG 3 cut(s) 1128, 2733, 3230
BstH2I RGCGCY 4 cut(s) 403, 1199, 1349, 3370
BstHHI GCGC 5 cut(s) 402, 1198, 1348, 1588, 3369
BstMWI GCNNNNNNNGC 5 cut(s) 622, 1345, 2288, 2893, 3251
BstNI CCWGG 8 cut(s) 493, 1521, 1884, 2568, 2630, 2798, 2927, 3262
BstNSI RCATGY 3 cut(s) 1046, 3193, 3334
BstPAI GACNNNNGTC 1 cut(s) 3285
BstSFI CTRYAG 2 cut(s) 894, 2580
BstSLI GKGCMC 2 cut(s) 2589, 2782
BstSNI TACGTA 1 cut(s) 1694
BstUI CGCG 3 cut(s) 1128, 2733, 3230
BstV1I GCAGC 8 cut(s) 210, 213, 699, 1891, 2017, 3078, 3229, 3235
BstV2I GAAGAC 1 cut(s) 1089
BstX2I RGATCY 6 cut(s) 1108, 2155, 2245, 2793, 2845, 2917
BstXI CCANNNNNNTGG 1 cut(s) 313
BstYI RGATCY 6 cut(s) 1108, 2155, 2245, 2793, 2845, 2917
BtgI CCRYGG 3 cut(s) 820, 1633, 2788
BtrI CACGTC 2 cut(s) 1650, 1747
BtsI GCAGTG 2 cut(s) 117, 1371
BtuMI TCGCGA 1 cut(s) 1128
BveI ACCTGC 3 cut(s) 1637, 1990, 2273
CaiI CAGNNNCTG 1 cut(s) 1526
CciI TCATGA 2 cut(s) 1063, 1539
CfoI GCGC 5 cut(s) 402, 1198, 1348, 1588, 3369
Cfr13I GGNCC 2 cut(s) 241, 2536
CseI GACGC 1 cut(s) 2483
Csp6I GTAC 8 cut(s) 500, 526, 590, 846, 984, 1256, 1691, 2286
CviQI GTAC 8 cut(s) 500, 526, 590, 846, 984, 1256, 1691, 2286
DrdI GACNNNNNNGTC 1 cut(s) 2602
DseDI GACNNNNNNGTC 1 cut(s) 2602
EaeI YGGCCR 9 cut(s) 186, 351, 817, 1309, 1517, 1644, 1870, 2626, 3013
Eam1104I CTCTTC 3 cut(s) 372, 2567, 2877
EarI CTCTTC 3 cut(s) 372, 2567, 2877
Eco105I TACGTA 1 cut(s) 1694
Eco130I CCWWGG 4 cut(s) 1633, 2684, 2788, 3032
Eco24I GRGCYC 1 cut(s) 3172
Eco31I GGTCTC 2 cut(s) 1669, 1775
Eco32I GATATC 5 cut(s) 294, 747, 1558, 1833, 2932
Eco47I GGWCC 2 cut(s) 241, 2536
Eco57I CTGAAG 3 cut(s) 165, 578, 669
Eco72I CACGTG 1 cut(s) 1433
EcoNI CCTNNNNNAGG 2 cut(s) 533, 1596
EcoRI GAATTC 4 cut(s) 668, 1969, 2018, 3179
EcoRII CCWGG 8 cut(s) 491, 1519, 1882, 2566, 2628, 2796, 2925, 3260
EcoRV GATATC 5 cut(s) 294, 747, 1558, 1833, 2932
EcoT14I CCWWGG 4 cut(s) 1633, 2684, 2788, 3032
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 3172
ErhI CCWWGG 4 cut(s) 1633, 2684, 2788, 3032
Esp3I CGTCTC 2 cut(s) 2466, 3060
FalI AAGNNNNNCTT 8 cut(s) 453, 485, 1098, 1130, 1948, 1980, 2871, 2903
FaqI GGGAC 2 cut(s) 330, 3129
FauI CCCGC 1 cut(s) 3106
FauNDI CATATG 4 cut(s) 1789, 2328, 2347, 2549
FbaI TGATCA 2 cut(s) 532, 2323
FblI GTMKAC 1 cut(s) 454
FriOI GRGCYC 1 cut(s) 3172
FspBI CTAG 6 cut(s) 665, 797, 1379, 2165, 2298, 2850
GlaI GCGC 5 cut(s) 401, 1197, 1347, 1587, 3368
GsaI CCCAGC 1 cut(s) 2805
GsuI CTGGAG 3 cut(s) 1408, 2419, 3003
HaeII RGCGCY 4 cut(s) 403, 1199, 1349, 3370
HapII CCGG 3 cut(s) 1214, 1873, 2446
HgaI GACGC 1 cut(s) 2483
HhaI GCGC 5 cut(s) 402, 1198, 1348, 1588, 3369
Hin6I GCGC 5 cut(s) 400, 1196, 1346, 1586, 3367
HinP1I GCGC 5 cut(s) 400, 1196, 1346, 1586, 3367
HincII GTYRAC 2 cut(s) 2716, 3267
HindII GTYRAC 2 cut(s) 2716, 3267
HindIII AAGCTT 2 cut(s) 1405, 2909
HpaII CCGG 3 cut(s) 1214, 1873, 2446
HphI GGTGA 9 cut(s) 74, 82, 527, 636, 929, 1613, 1898, 2711, 3056
Hpy166II GTNNAC 6 cut(s) 455, 848, 1817, 2587, 2716, 3267
Hpy8I GTNNAC 6 cut(s) 455, 848, 1817, 2587, 2716, 3267
Hpy99I CGWCG 3 cut(s) 140, 143, 1128
HpyCH4III ACNGT 7 cut(s) 326, 521, 925, 1323, 2321, 2722, 3287
HpyCH4IV ACGT 6 cut(s) 1432, 1649, 1693, 1746, 2662, 3352
HpyF10VI GCNNNNNNNGC 5 cut(s) 622, 1345, 2288, 2893, 3251
HpySE526I ACGT 6 cut(s) 1432, 1649, 1693, 1746, 2662, 3352
HspAI GCGC 5 cut(s) 400, 1196, 1346, 1586, 3367
Kpn2I TCCGGA 1 cut(s) 2445
KpnI GGTACC 1 cut(s) 503
Ksp22I TGATCA 2 cut(s) 532, 2323
LguI GCTCTTC 1 cut(s) 2877
LmnI GCTCC 7 cut(s) 124, 824, 1168, 1193, 1427, 1513, 3130
Lsp1109I GCAGC 8 cut(s) 210, 213, 699, 1891, 2017, 3078, 3229, 3235
MaeI CTAG 6 cut(s) 665, 797, 1379, 2165, 2298, 2850
MaeII ACGT 6 cut(s) 1432, 1649, 1693, 1746, 2662, 3352
MaeIII GTNAC 7 cut(s) 233, 515, 833, 1892, 2519, 2663, 3319
MbiI CCGCTC 3 cut(s) 17, 1516, 3113
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 6 cut(s) 1108, 2155, 2245, 2793, 2845, 2917
MhlI GDGCHC 5 cut(s) 1240, 1432, 2589, 2782, 3172
MlsI TGGCCA 5 cut(s) 188, 353, 1311, 1646, 2628
MluNI TGGCCA 5 cut(s) 188, 353, 1311, 1646, 2628
MlyI GAGTC 6 cut(s) 809, 917, 1954, 2553, 3047, 3262
MmeI TCCRAC 6 cut(s) 81, 159, 1430, 1487, 2799, 3181
Mox20I TGGCCA 5 cut(s) 188, 353, 1311, 1646, 2628
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 2445
MscI TGGCCA 5 cut(s) 188, 353, 1311, 1646, 2628
MseI TTAA 9 cut(s) 1139, 1473, 1581, 2082, 2468, 2505, 2877, 2889, 3379
MslI CAYNNNNRTG 1 cut(s) 1444
Msp20I TGGCCA 5 cut(s) 188, 353, 1311, 1646, 2628
MspA1I CMGCKG 3 cut(s) 1457, 1563, 2030
MspCI CTTAAG 2 cut(s) 2081, 2467
MspI CCGG 3 cut(s) 1214, 1873, 2446
MunI CAATTG 1 cut(s) 720
Mva1269I GAATGC 3 cut(s) 556, 827, 1168
MvaI CCWGG 8 cut(s) 493, 1521, 1884, 2568, 2630, 2798, 2927, 3262
MvnI CGCG 3 cut(s) 1128, 2733, 3230
MwoI GCNNNNNNNGC 5 cut(s) 622, 1345, 2288, 2893, 3251
NciI CCSGG 2 cut(s) 1215, 1874
NcoI CCATGG 2 cut(s) 1633, 2788
NdeI CATATG 4 cut(s) 1789, 2328, 2347, 2549
NlaIV GGNNCC 8 cut(s) 363, 501, 2188, 2247, 2779, 2795, 2847, 3073
NmeAIII GCCGAG 1 cut(s) 1828
NmuCI GTSAC 4 cut(s) 233, 515, 2519, 3319
NruI TCGCGA 1 cut(s) 1128
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 1046, 3193, 3334
NspV TTCGAA 1 cut(s) 159
PaeI GCATGC 1 cut(s) 3193
PagI TCATGA 2 cut(s) 1063, 1539
PciI ACATGT 1 cut(s) 1042
PciSI GCTCTTC 1 cut(s) 2877
PctI GAATGC 3 cut(s) 556, 827, 1168
PfoI TCCNGGA 1 cut(s) 491
PleI GAGTC 6 cut(s) 808, 916, 1954, 2553, 3046, 3262
PmaCI CACGTG 1 cut(s) 1433
PmlI CACGTG 1 cut(s) 1433
PpsI GAGTC 6 cut(s) 808, 916, 1954, 2553, 3046, 3262
Ppu21I YACGTR 2 cut(s) 1433, 1694
PscI ACATGT 1 cut(s) 1042
PshAI GACNNNNGTC 1 cut(s) 3285
PshBI ATTAAT 1 cut(s) 1139
PsiI TTATAA 2 cut(s) 933, 1625
Psp6I CCWGG 8 cut(s) 491, 1519, 1882, 2566, 2628, 2796, 2925, 3260
PspCI CACGTG 1 cut(s) 1433
PspFI CCCAGC 1 cut(s) 2801
PspGI CCWGG 8 cut(s) 491, 1519, 1882, 2566, 2628, 2796, 2925, 3260
PspN4I GGNNCC 8 cut(s) 363, 501, 2188, 2247, 2779, 2795, 2847, 3073
PspPI GGNCC 2 cut(s) 241, 2536
PstI CTGCAG 1 cut(s) 898
PstNI CAGNNNCTG 1 cut(s) 1526
PsuI RGATCY 6 cut(s) 1108, 2155, 2245, 2793, 2845, 2917
PvuII CAGCTG 3 cut(s) 1457, 1563, 2030
RruI TCGCGA 1 cut(s) 1128
RsaI GTAC 8 cut(s) 501, 527, 591, 847, 985, 1257, 1692, 2287
RsaNI GTAC 8 cut(s) 500, 526, 590, 846, 984, 1256, 1691, 2286
RseI CAYNNNNRTG 1 cut(s) 1444
SapI GCTCTTC 1 cut(s) 2877
SaqAI TTAA 9 cut(s) 1139, 1473, 1581, 2082, 2468, 2505, 2877, 2889, 3379
Sau96I GGNCC 2 cut(s) 241, 2536
SchI GAGTC 6 cut(s) 809, 917, 1954, 2553, 3047, 3262
SduI GDGCHC 5 cut(s) 1240, 1432, 2589, 2782, 3172
SfcI CTRYAG 2 cut(s) 894, 2580
SfuI TTCGAA 1 cut(s) 159
SinI GGWCC 2 cut(s) 241, 2536
SmiMI CAYNNNNRTG 1 cut(s) 1444
SmlI CTYRAG 5 cut(s) 1027, 2081, 2262, 2467, 3149
SmoI CTYRAG 5 cut(s) 1027, 2081, 2262, 2467, 3149
SnaBI TACGTA 1 cut(s) 1694
SphI GCATGC 1 cut(s) 3193
SspI AATATT 1 cut(s) 2135
SspMI CTAG 6 cut(s) 665, 797, 1379, 2165, 2298, 2850
StyI CCWWGG 4 cut(s) 1633, 2684, 2788, 3032
TaaI ACNGT 7 cut(s) 326, 521, 925, 1323, 2321, 2722, 3287
TaiI ACGT 6 cut(s) 1435, 1652, 1696, 1749, 2665, 3355
TaqI TCGA 4 cut(s) 11, 159, 856, 940
TatI WGTACW 2 cut(s) 845, 1255
TauI GCSGC 3 cut(s) 20, 114, 1519
Tru1I TTAA 9 cut(s) 1139, 1473, 1581, 2082, 2468, 2505, 2877, 2889, 3379
Tru9I TTAA 9 cut(s) 1139, 1473, 1581, 2082, 2468, 2505, 2877, 2889, 3379
TseFI GTSAC 4 cut(s) 233, 515, 2519, 3319
TseI GCWGC 8 cut(s) 223, 226, 712, 1879, 2030, 3091, 3223, 3242
Tsp45I GTSAC 4 cut(s) 233, 515, 2519, 3319
TspGWI ACGGA 2 cut(s) 2528, 3084
Vha464I CTTAAG 2 cut(s) 2081, 2467
VneI GTGCAC 1 cut(s) 2585
VpaK11BI GGWCC 2 cut(s) 241, 2536
VspI ATTAAT 1 cut(s) 1139
XagI CCTNNNNNAGG 2 cut(s) 533, 1596
XapI RAATTY 8 cut(s) 668, 1059, 1181, 1969, 2018, 2113, 2404, 3179
XbaI TCTAGA 1 cut(s) 2297
XceI RCATGY 3 cut(s) 1046, 3193, 3334
XmiI GTMKAC 1 cut(s) 454
XspI CTAG 6 cut(s) 665, 797, 1379, 2165, 2298, 2850
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.