Rh4AG135700

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005499
Physical Location & Seq
Forward (+)
29795551 .. 29810728
15178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG135700.1

Sequence Viewer

Length: 3081 bp
ATGAGAGCGGCGGCGTCTCTTCTTGTCAAATCAGTAGCCTCCTCCAACAATACTTGTTTTCGTTCTCACCACCTCAAATTCTGTCTTGTTGTACTCCAATCACAACCACACTCCTCTTCTTCTTCTTCTTCTTCTTCTTCCTCTTCTCCCATTTCGATTTCGAAGCGCGGCTTTTCCGGCTACGCTGCGGAGCAGTTCTCCGACGACGAGTATGAGTGCGATTTCGAAAGCCAAAAGGCTTCATCTTCCGTGGCCAACATTGACGAGTGGAAATGGAAACTGAGCCTGCTGCTGCGCAGTGACAAGGACCAAGAGATTGTTTCCAGAGATAAAAGGGATAGGAGAGACTACGAGCAAATATCTAACCTCGCCAAACGGATGGGACTTTACACTGAAATATATGGCAAAGTGGTGGTTGCCAGCAAGGTTCCTCTTCCAAACTACCGCCCCGATTTGGATGATAAGCGCCCACAAAGAGAGGTGGTTATCCCTCTTGGCTTGCAAAGGAGGGTAGAGGGTTTACTGCAAGAACACCTTGATCGTGTCCTGCTAAGTTCCCGCAACGTATCAGCCAATTCAGGTGGTGACAGTGAGCACTTCGATCAGGTTGAAAATGATAAGCCTGATGCGAATGCAGATTCTTTTCTTGATGGGTCTGTAATGGAGAAGGTTCTTCAGCGTAGGAGTTTGCGCATGCGTAATATGCAAAGAGCTTGGCAGGAATCACCTGAAGGTAAAAAGATGCTAGAGTTCCGGAAATCACTACCATCTTTCAAAGAGAATCAGAGGTTGCTGCAAGCAATAGCACAAAATCAGGTAGTAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAGCTTCCTCAATATATACTAGAGTCAGAGATAGAATCTGGCCGTGGAGCATTCTGTAGCATTATCTGTACACAACCTCGAAGAATATCTGCTATGGCTGTTGCTGAAAGAGTGTCTGCAGAGAGGGGAGAGCCTCTTGGTGAAACAGTGGGCTATAAAGTTCGATTAGAGGGAATGAAAGGAAAAAATACGCATCTCCTTTTCTGTACCAGTGGTATTCTACTTCGCCGTCTGTTGAGTGATCGGAACTTGAGTGGTGTAACACATGTTTTTGTTGATGAAATTCATGAACGAGGCATGAATGAAGACTTCTTGTTGATTGTATTGAAAGATCTGCTTCCACGCCGTCGGGATCTAAGATTGGTTTTGATGAGTGCCACTCTGAATGCGGAGCTATTTTCAAATTTTTTTGGAGGAGCACCAACAATTCACATTCCGGGTTTCACATATCCAGTGAGAGCACACTTTTTGGAAGATGCACTGGAAATGACTGGGTATAAGCTGACTTCTTTCAACCAAATTGATGACTATGGCCAAGACAAACTGTGGAAAACGCAGAAGCAGTTAGCGCCTCGGAAAAGGAAAAACCAGATCACTGCTCTAGTTGAGGATGCTCTTAGTAAATCGAGTTTTGAGAATTATAGCCCCAGAGCACGCGATTCATTATCATGTTGGACGGCTGATTGCATAGGATTTAATCTTATTGAAGCAGTTTTATGTCATATATGTCGGAAGGAACGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGAGGATATCAGCTGTTTGAGGGATCAACTTAAAGCCCATCCTCTTTTAGGTGATCCTAACAGGGTTTTGCTTATAACATGCCATGGGTCAATGACCACGTCTGAACAGAACCTCATTTTCGCGAGACCGCCTCCTAATGTACGTAAAATTGTTCTTGCTACTAATATGGCAGAAGCAAGCATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCACATATGATGCTTTGAATAATACACCTTGTCTACTACCTTCTTGGATATCCCAAGCATCTGCTCGGCAAAGAAGGGGAAGGGCTGGCCGGGTGCAGCCAGGCGAATGTTACCACCTTTACCCCAAATGTGTGTATCATGCGTTTGCAGAATACCAACTTCCTGAACTCCTAAGGACTCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTTTGCAGGTTGGTAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCACCTGAACCCCTTGCTGTTCATAATGCTGTTGGCTTTCTTAAGTTGATTGGGGCATTGGATGAGAATGAAAATTTGACAAATCTCGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTAAGTGTCCGGGATCCATTCCTTTTGCCTCAAGACAAAAAAGATTTAGCGGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTATAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCAGAAAGAGAAGGATCAGCATATGAATACTGCTGGAGAAATTTTCTCTCTGCCCAGACACTTCAAGCTATTCATTCTCTTCGGAAGCAGTTTAGCTACATCTTAAGAGACGCTGGCTTGGTGGATGCAGATGCAAGCATTAACAACAAGCTAAGTCACAATCAGTCATTGGTCCGCGCAATCATATGTTCCGGACTATTCCCTGGCATAGCATCTGTAGTGCACCGAGAGACATCCATGTCTTTCAAAACTATGGATGATGGCCAGGTTTTACTATATGCTAATTCTGTAAATGCACGTTACCAAACAATTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAGTAAAGGTCAACACAGTTTTCATACGCGATTCTACTGGTGTCTCTGATTCCATTTTGATCCTTTTTGGTGGTGCCCTAAACCATGGTATCCAGGCTGGGCATTTGAAAATGTTGGATGGCTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGCTTAAAGAAGAGCTTAATGAGCTTATTCAGAAGAAGGTGAGTTTGGATATGATTTTACTTGAAGAAAAAACATGTCATACATCATCCGCAACCCATCCGGATTTGTTACACTCATTTTTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1026

Amino Acids

114.99

Weight (kDa)

8.06

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 263 - 417 1.9e-09 DEAD/DEAH box helicase
Helicase_C PF00271 524 - 653 3.1e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 717 - 745 2.5e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 746 - 806 5.1e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 873 - 954 4.7e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 963 - 998 2.9e-11 C-terminal domain in RNA helicases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1706
AasI GACNNNNNNGTC 1 cut(s) 2683
Acc16I TGCGCA 2 cut(s) 296, 692
Acc36I ACCTGC 1 cut(s) 2071
AccB1I GGYRCC 1 cut(s) 2858
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 1897
AccII CGCG 5 cut(s) 168, 1515, 1754, 2622, 2814
AccIII TCCGGA 3 cut(s) 753, 2636, 3052
AcoI YGGCCR 6 cut(s) 252, 898, 1390, 1598, 1951, 2707
AcsI RAATTY 7 cut(s) 77, 1140, 1261, 2050, 2099, 2194, 2485
AcuI CTGAAG 2 cut(s) 659, 750
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 4 cut(s) 93, 928, 1066, 1773
AfiI CCNNNNNNNGG 6 cut(s) 454, 1679, 2246, 2247, 2766, 2770
AflII CTTAAG 2 cut(s) 2162, 2548
AflIII ACRYGT 2 cut(s) 1123, 3026
AjiI CACGTC 1 cut(s) 1731
AjnI CCWGG 5 cut(s) 1600, 1963, 2647, 2709, 2877
AjuI GAANNNNNNNTTGG 2 cut(s) 2981, 3013
Alw21I GWGCWC 5 cut(s) 597, 1279, 1321, 1513, 2670
Alw26I GTCTC 8 cut(s) 21, 339, 830, 1750, 1856, 2547, 2669, 2833
Alw44I GTGCAC 1 cut(s) 2666
AlwNI CAGNNNCTG 1 cut(s) 1607
Aor13HI TCCGGA 3 cut(s) 753, 2636, 3052
AoxI GGCC 6 cut(s) 252, 898, 1390, 1598, 1951, 2707
ApaLI GTGCAC 1 cut(s) 2666
ApeKI GCWGC 6 cut(s) 185, 289, 292, 793, 1960, 2111
ApoI RAATTY 7 cut(s) 77, 1140, 1261, 2050, 2099, 2194, 2485
AspLEI GCGC 6 cut(s) 168, 297, 468, 693, 1429, 2624
AspS9I GGNCC 2 cut(s) 307, 2617
AsuC2I CCSGG 3 cut(s) 1296, 1955, 2324
AsuHPI GGTGA 7 cut(s) 59, 596, 717, 1010, 1694, 2792, 3004
AsuII TTCGAA 2 cut(s) 161, 225
AvaII GGWCC 2 cut(s) 307, 2617
AxyI CCTNAGG 1 cut(s) 2036
BaeGI GKGCMC 2 cut(s) 2670, 2863
BaeI ACNNNNGTAYC 2 cut(s) 2857, 2890
BalI TGGCCA 3 cut(s) 254, 1392, 2709
BamHI GGATCC 2 cut(s) 2326, 2926
BanI GGYRCC 1 cut(s) 2858
BbsI GAAGAC 1 cut(s) 1170
Bbv12I GWGCWC 5 cut(s) 597, 1279, 1321, 1513, 2670
BbvI GCAGC 6 cut(s) 172, 276, 279, 780, 1972, 2098
BccI CCATC 9 cut(s) 373, 644, 775, 1677, 2430, 2699, 2897, 2932, 3057
BceAI ACGGC 5 cut(s) 885, 1071, 1188, 1551, 1613
BciT130I CCWGG 5 cut(s) 1602, 1965, 2649, 2711, 2879
BciVI GTATCC 1 cut(s) 2885
BclI TGATCA 1 cut(s) 2404
BcnI CCSGG 3 cut(s) 1296, 1955, 2324
BcoDI GTCTC 8 cut(s) 21, 339, 830, 1750, 1856, 2547, 2669, 2833
BfaI CTAG 6 cut(s) 746, 878, 1460, 2246, 2379, 2931
BfmI CTRYAG 3 cut(s) 913, 975, 2661
BfoI RGCGCY 2 cut(s) 469, 1430
BfrI CTTAAG 2 cut(s) 2162, 2548
BfuAI ACCTGC 1 cut(s) 2071
BfuI GTATCC 1 cut(s) 2885
BglII AGATCT 1 cut(s) 1189
BisI GCNGC 9 cut(s) 9, 12, 169, 186, 290, 293, 794, 1961, 2112
BlsI GCNGC 9 cut(s) 10, 13, 170, 187, 291, 294, 795, 1962, 2113
Bme1390I CCNGG 8 cut(s) 1296, 1602, 1955, 1965, 2324, 2649, 2711, 2879
Bme18I GGWCC 2 cut(s) 307, 2617
BmgBI CACGTC 1 cut(s) 1731
BmgT120I GGNCC 2 cut(s) 307, 2617
BmiI GGNNCC 4 cut(s) 429, 2328, 2860, 2928
BmrFI CCNGG 8 cut(s) 1296, 1602, 1955, 1965, 2324, 2649, 2711, 2879
BmrI ACTGGG 2 cut(s) 1359, 1635
BmuI ACTGGG 2 cut(s) 1359, 1635
BpiI GAAGAC 1 cut(s) 1170
BplI GAGNNNNNCTC 6 cut(s) 182, 214, 1222, 1254, 1747, 1779
BpmI CTGGAG 1 cut(s) 2500
Bpu14I TTCGAA 2 cut(s) 161, 225
BpuEI CTTGAG 2 cut(s) 1129, 2328
BpuMI CCSGG 3 cut(s) 1296, 1955, 2324
BsaAI YACGTR 1 cut(s) 1775
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 2 cut(s) 1750, 1856
BsaJI CCNNGG 7 cut(s) 249, 901, 1430, 1714, 2647, 2765, 2869
BsaWI WCCGGW 3 cut(s) 753, 2636, 3052
Bsc4I CCNNNNNNNGG 6 cut(s) 454, 1679, 2246, 2247, 2766, 2770
Bse1I ACTGG 8 cut(s) 844, 1068, 1310, 1344, 1354, 1630, 2231, 2827
Bse21I CCTNAGG 1 cut(s) 2036
Bse3DI GCAATG 1 cut(s) 2278
BseAI TCCGGA 3 cut(s) 753, 2636, 3052
BseBI CCWGG 5 cut(s) 1602, 1965, 2649, 2711, 2879
BseDI CCNNGG 7 cut(s) 249, 901, 1430, 1714, 2647, 2765, 2869
BseLI CCNNNNNNNGG 6 cut(s) 454, 1679, 2246, 2247, 2766, 2770
BseMI GCAATG 1 cut(s) 2278
BseMII CTCAG 2 cut(s) 272, 872
BseNI ACTGG 8 cut(s) 844, 1068, 1310, 1344, 1354, 1630, 2231, 2827
BseRI GAGGAG 3 cut(s) 31, 103, 1287
BseSI GKGCMC 2 cut(s) 2670, 2863
BseXI GCAGC 6 cut(s) 172, 276, 279, 780, 1972, 2098
BseYI CCCAGC 1 cut(s) 2882
BsgI GTGCAG 1 cut(s) 1979
Bsh1236I CGCG 5 cut(s) 168, 1515, 1754, 2622, 2814
BshFI GGCC 6 cut(s) 254, 900, 1392, 1600, 1953, 2709
BshNI GGYRCC 1 cut(s) 2858
BsiHKAI GWGCWC 5 cut(s) 597, 1279, 1321, 1513, 2670
BsiSI CCGG 7 cut(s) 177, 754, 1295, 1954, 2323, 2637, 3053
BslFI GGGAC 2 cut(s) 396, 2380
BslI CCNNNNNNNGG 6 cut(s) 454, 1679, 2246, 2247, 2766, 2770
BsmAI GTCTC 8 cut(s) 21, 339, 830, 1750, 1856, 2547, 2669, 2833
BsmBI CGTCTC 2 cut(s) 21, 2547
BsmFI GGGAC 2 cut(s) 396, 2380
BsmI GAATGC 3 cut(s) 637, 908, 1249
BsnI GGCC 6 cut(s) 254, 900, 1392, 1600, 1953, 2709
Bso31I GGTCTC 2 cut(s) 1750, 1856
Bsp119I TTCGAA 2 cut(s) 161, 225
Bsp1286I GDGCHC 6 cut(s) 597, 1279, 1321, 1513, 2670, 2863
Bsp13I TCCGGA 3 cut(s) 753, 2636, 3052
Bsp1407I TGTACA 1 cut(s) 926
Bsp19I CCATGG 2 cut(s) 1714, 2869
Bsp68I TCGCGA 1 cut(s) 1754
BspANI GGCC 6 cut(s) 254, 900, 1392, 1600, 1953, 2709
BspCNI CTCAG 2 cut(s) 273, 871
BspEI TCCGGA 3 cut(s) 753, 2636, 3052
BspFNI CGCG 5 cut(s) 168, 1515, 1754, 2622, 2814
BspHI TCATGA 2 cut(s) 1144, 1620
BspLI GGNNCC 4 cut(s) 429, 2328, 2860, 2928
BspMAI CTGCAG 1 cut(s) 979
BspMI ACCTGC 1 cut(s) 2071
BspQI GCTCTTC 1 cut(s) 2958
BspT104I TTCGAA 2 cut(s) 161, 225
BspT107I GGYRCC 1 cut(s) 2858
BspTI CTTAAG 2 cut(s) 2162, 2548
BspTNI GGTCTC 2 cut(s) 1750, 1856
BsrBI CCGCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 2278
BsrGI TGTACA 1 cut(s) 926
BsrI ACTGG 8 cut(s) 844, 1068, 1310, 1344, 1354, 1630, 2231, 2827
BssECI CCNNGG 7 cut(s) 249, 901, 1430, 1714, 2647, 2765, 2869
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 3 cut(s) 1714, 2765, 2869
Bst2UI CCWGG 5 cut(s) 1602, 1965, 2649, 2711, 2879
Bst4CI ACNGT 4 cut(s) 590, 1006, 1404, 2803
Bst6I CTCTTC 6 cut(s) 24, 121, 148, 438, 2529, 2958
BstACI GRCGYC 1 cut(s) 14
BstAFI CTTAAG 2 cut(s) 2162, 2548
BstAUI TGTACA 1 cut(s) 926
BstBAI YACGTR 1 cut(s) 1775
BstBI TTCGAA 2 cut(s) 161, 225
BstDEI CTNAG 9 cut(s) 281, 551, 858, 1214, 1475, 1612, 2036, 2315, 2597
BstDSI CCRYGG 4 cut(s) 249, 901, 1714, 2869
BstENI CCTNNNNNAGG 1 cut(s) 1677
BstFNI CGCG 5 cut(s) 168, 1515, 1754, 2622, 2814
BstH2I RGCGCY 2 cut(s) 469, 1430
BstHHI GCGC 6 cut(s) 168, 297, 468, 693, 1429, 2624
BstMAI GTCTC 8 cut(s) 21, 339, 830, 1750, 1856, 2547, 2669, 2833
BstMWI GCNNNNNNNGC 5 cut(s) 177, 703, 1426, 2369, 2974
BstNI CCWGG 5 cut(s) 1602, 1965, 2649, 2711, 2879
BstNSI RCATGY 4 cut(s) 697, 1127, 1713, 3030
BstSCI CCNGG 8 cut(s) 1294, 1600, 1953, 1963, 2322, 2647, 2709, 2877
BstSFI CTRYAG 3 cut(s) 913, 975, 2661
BstSLI GKGCMC 2 cut(s) 2670, 2863
BstSNI TACGTA 1 cut(s) 1775
BstUI CGCG 5 cut(s) 168, 1515, 1754, 2622, 2814
BstV1I GCAGC 6 cut(s) 172, 276, 279, 780, 1972, 2098
BstV2I GAAGAC 1 cut(s) 1170
BstX2I RGATCY 5 cut(s) 1189, 1210, 2236, 2326, 2926
BstXI CCANNNNNNTGG 1 cut(s) 379
BstYI RGATCY 5 cut(s) 1189, 1210, 2236, 2326, 2926
Bsu36I CCTNAGG 1 cut(s) 2036
BsuI GTATCC 1 cut(s) 2885
BsuRI GGCC 6 cut(s) 254, 900, 1392, 1600, 1953, 2709
BtgI CCRYGG 4 cut(s) 249, 901, 1714, 2869
BtrI CACGTC 1 cut(s) 1731
BtsI GCAGTG 2 cut(s) 304, 1452
BtsIMutI CAGTG 8 cut(s) 304, 390, 595, 1011, 1075, 1317, 1337, 1452
BtuMI TCGCGA 1 cut(s) 1754
BveI ACCTGC 1 cut(s) 2071
CaiI CAGNNNCTG 1 cut(s) 1607
CciI TCATGA 2 cut(s) 1144, 1620
CfoI GCGC 6 cut(s) 168, 297, 468, 693, 1429, 2624
Cfr13I GGNCC 2 cut(s) 307, 2617
CseI GACGC 2 cut(s) 3, 2564
Csp6I GTAC 4 cut(s) 92, 927, 1065, 1772
CspCI CAANNNNNGTGG 2 cut(s) 562, 597
CviQI GTAC 4 cut(s) 92, 927, 1065, 1772
DdeI CTNAG 9 cut(s) 281, 551, 858, 1214, 1475, 1612, 2036, 2315, 2597
DrdI GACNNNNNNGTC 1 cut(s) 2683
DseDI GACNNNNNNGTC 1 cut(s) 2683
EaeI YGGCCR 6 cut(s) 252, 898, 1390, 1598, 1951, 2707
Eam1104I CTCTTC 6 cut(s) 24, 121, 148, 438, 2529, 2958
EarI CTCTTC 6 cut(s) 24, 121, 148, 438, 2529, 2958
Eco105I TACGTA 1 cut(s) 1775
Eco130I CCWWGG 3 cut(s) 1714, 2765, 2869
Eco31I GGTCTC 2 cut(s) 1750, 1856
Eco32I GATATC 3 cut(s) 828, 1639, 1914
Eco47I GGWCC 2 cut(s) 307, 2617
Eco57I CTGAAG 2 cut(s) 659, 750
Eco81I CCTNAGG 1 cut(s) 2036
EcoNI CCTNNNNNAGG 1 cut(s) 1677
EcoRI GAATTC 2 cut(s) 2050, 2099
EcoRII CCWGG 5 cut(s) 1600, 1963, 2647, 2709, 2877
EcoRV GATATC 3 cut(s) 828, 1639, 1914
EcoT14I CCWWGG 3 cut(s) 1714, 2765, 2869
ErhI CCWWGG 3 cut(s) 1714, 2765, 2869
Esp3I CGTCTC 2 cut(s) 21, 2547
FaqI GGGAC 2 cut(s) 396, 2380
FauI CCCGC 2 cut(s) 566, 2356
FauNDI CATATG 5 cut(s) 1870, 2409, 2428, 2467, 2630
FbaI TGATCA 1 cut(s) 2404
FblI GTMKAC 1 cut(s) 1897
Fnu4HI GCNGC 9 cut(s) 9, 12, 169, 186, 290, 293, 794, 1961, 2112
Fsp4HI GCNGC 9 cut(s) 9, 12, 169, 186, 290, 293, 794, 1961, 2112
FspBI CTAG 6 cut(s) 746, 878, 1460, 2246, 2379, 2931
FspI TGCGCA 2 cut(s) 296, 692
GlaI GCGC 6 cut(s) 167, 296, 467, 692, 1428, 2623
GluI GCNGC 9 cut(s) 9, 12, 169, 186, 290, 293, 794, 1961, 2112
GsaI CCCAGC 1 cut(s) 2886
GsuI CTGGAG 1 cut(s) 2500
HaeII RGCGCY 2 cut(s) 469, 1430
HaeIII GGCC 6 cut(s) 254, 900, 1392, 1600, 1953, 2709
HapII CCGG 7 cut(s) 177, 754, 1295, 1954, 2323, 2637, 3053
HgaI GACGC 2 cut(s) 3, 2564
HhaI GCGC 6 cut(s) 168, 297, 468, 693, 1429, 2624
Hin1I GRCGYC 1 cut(s) 14
Hin6I GCGC 6 cut(s) 166, 295, 466, 691, 1427, 2622
HinP1I GCGC 6 cut(s) 166, 295, 466, 691, 1427, 2622
HincII GTYRAC 1 cut(s) 2797
HindII GTYRAC 1 cut(s) 2797
HindIII AAGCTT 1 cut(s) 2954
HpaII CCGG 7 cut(s) 177, 754, 1295, 1954, 2323, 2637, 3053
HphI GGTGA 7 cut(s) 59, 596, 717, 1010, 1694, 2792, 3004
Hpy166II GTNNAC 5 cut(s) 521, 929, 1898, 2668, 2797
Hpy8I GTNNAC 5 cut(s) 521, 929, 1898, 2668, 2797
Hpy99I CGWCG 3 cut(s) 206, 209, 1209
HpyAV CCTTC 9 cut(s) 661, 725, 1585, 1914, 1932, 1938, 2426, 2450, 2983
HpyCH4III ACNGT 4 cut(s) 590, 1006, 1404, 2803
HpyCH4IV ACGT 4 cut(s) 564, 1730, 1774, 2743
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 703, 1426, 2369, 2974
HpyF3I CTNAG 9 cut(s) 281, 551, 858, 1214, 1475, 1612, 2036, 2315, 2597
HpySE526I ACGT 4 cut(s) 564, 1730, 1774, 2743
Hsp92I GRCGYC 1 cut(s) 14
HspAI GCGC 6 cut(s) 166, 295, 466, 691, 1427, 2622
Kpn2I TCCGGA 3 cut(s) 753, 2636, 3052
Ksp22I TGATCA 1 cut(s) 2404
LguI GCTCTTC 1 cut(s) 2958
LmnI GCTCC 4 cut(s) 190, 905, 1249, 1274
Lsp1109I GCAGC 6 cut(s) 172, 276, 279, 780, 1972, 2098
MaeI CTAG 6 cut(s) 746, 878, 1460, 2246, 2379, 2931
MaeII ACGT 4 cut(s) 564, 1730, 1774, 2743
MaeIII GTNAC 7 cut(s) 299, 584, 1117, 1973, 2600, 2744, 3060
MbiI CCGCTC 1 cut(s) 8
MflI RGATCY 5 cut(s) 1189, 1210, 2236, 2326, 2926
MhlI GDGCHC 6 cut(s) 597, 1279, 1321, 1513, 2670, 2863
MlsI TGGCCA 3 cut(s) 254, 1392, 2709
MluNI TGGCCA 3 cut(s) 254, 1392, 2709
MlyI GAGTC 2 cut(s) 890, 2035
MmeI TCCRAC 5 cut(s) 69, 225, 1511, 1568, 2880
Mox20I TGGCCA 3 cut(s) 254, 1392, 2709
MroI TCCGGA 3 cut(s) 753, 2636, 3052
MscI TGGCCA 3 cut(s) 254, 1392, 2709
MseI TTAA 7 cut(s) 1554, 1662, 2163, 2549, 2586, 2958, 2970
MslI CAYNNNNRTG 1 cut(s) 1525
Msp20I TGGCCA 3 cut(s) 254, 1392, 2709
MspA1I CMGCKG 2 cut(s) 1644, 2111
MspCI CTTAAG 2 cut(s) 2162, 2548
MspI CCGG 7 cut(s) 177, 754, 1295, 1954, 2323, 2637, 3053
MspR9I CCNGG 8 cut(s) 1296, 1602, 1955, 1965, 2324, 2649, 2711, 2879
Mva1269I GAATGC 3 cut(s) 637, 908, 1249
MvaI CCWGG 5 cut(s) 1602, 1965, 2649, 2711, 2879
MvnI CGCG 5 cut(s) 168, 1515, 1754, 2622, 2814
MwoI GCNNNNNNNGC 5 cut(s) 177, 703, 1426, 2369, 2974
NciI CCSGG 3 cut(s) 1296, 1955, 2324
NcoI CCATGG 2 cut(s) 1714, 2869
NdeI CATATG 5 cut(s) 1870, 2409, 2428, 2467, 2630
NlaIV GGNNCC 4 cut(s) 429, 2328, 2860, 2928
NmeAIII GCCGAG 1 cut(s) 1909
NmuCI GTSAC 3 cut(s) 299, 584, 2600
NruI TCGCGA 1 cut(s) 1754
NsbI TGCGCA 2 cut(s) 296, 692
NspI RCATGY 4 cut(s) 697, 1127, 1713, 3030
NspV TTCGAA 2 cut(s) 161, 225
PaeI GCATGC 1 cut(s) 697
PagI TCATGA 2 cut(s) 1144, 1620
PciI ACATGT 2 cut(s) 1123, 3026
PciSI GCTCTTC 1 cut(s) 2958
PctI GAATGC 3 cut(s) 637, 908, 1249
PfeI GAWTC 8 cut(s) 638, 722, 781, 893, 1517, 2382, 2816, 2834
PflFI GACNNNGTC 1 cut(s) 1729
PfoI TCCNGGA 1 cut(s) 2322
PkrI GCNGC 9 cut(s) 10, 13, 170, 187, 291, 294, 795, 1962, 2113
PleI GAGTC 2 cut(s) 889, 2035
PpsI GAGTC 2 cut(s) 889, 2035
Ppu21I YACGTR 1 cut(s) 1775
PscI ACATGT 2 cut(s) 1123, 3026
PsiI TTATAA 1 cut(s) 1706
Psp6I CCWGG 5 cut(s) 1600, 1963, 2647, 2709, 2877
PspFI CCCAGC 1 cut(s) 2882
PspGI CCWGG 5 cut(s) 1600, 1963, 2647, 2709, 2877
PspN4I GGNNCC 4 cut(s) 429, 2328, 2860, 2928
PspPI GGNCC 2 cut(s) 307, 2617
PstI CTGCAG 1 cut(s) 979
PstNI CAGNNNCTG 1 cut(s) 1607
PsuI RGATCY 5 cut(s) 1189, 1210, 2236, 2326, 2926
PsyI GACNNNGTC 1 cut(s) 1729
PvuII CAGCTG 2 cut(s) 1644, 2111
RruI TCGCGA 1 cut(s) 1754
RsaI GTAC 4 cut(s) 93, 928, 1066, 1773
RsaNI GTAC 4 cut(s) 92, 927, 1065, 1772
RseI CAYNNNNRTG 1 cut(s) 1525
SapI GCTCTTC 1 cut(s) 2958
SaqAI TTAA 7 cut(s) 1554, 1662, 2163, 2549, 2586, 2958, 2970
SatI GCNGC 9 cut(s) 9, 12, 169, 186, 290, 293, 794, 1961, 2112
Sau96I GGNCC 2 cut(s) 307, 2617
SchI GAGTC 2 cut(s) 890, 2035
ScrFI CCNGG 8 cut(s) 1296, 1602, 1955, 1965, 2324, 2649, 2711, 2879
SduI GDGCHC 6 cut(s) 597, 1279, 1321, 1513, 2670, 2863
SfcI CTRYAG 3 cut(s) 913, 975, 2661
SfuI TTCGAA 2 cut(s) 161, 225
SinI GGWCC 2 cut(s) 307, 2617
SmiMI CAYNNNNRTG 1 cut(s) 1525
SmlI CTYRAG 4 cut(s) 1108, 2162, 2343, 2548
SmoI CTYRAG 4 cut(s) 1108, 2162, 2343, 2548
SnaBI TACGTA 1 cut(s) 1775
SphI GCATGC 1 cut(s) 697
SspI AATATT 1 cut(s) 2216
SspMI CTAG 6 cut(s) 746, 878, 1460, 2246, 2379, 2931
StyD4I CCNGG 8 cut(s) 1294, 1600, 1953, 1963, 2322, 2647, 2709, 2877
StyI CCWWGG 3 cut(s) 1714, 2765, 2869
TaaI ACNGT 4 cut(s) 590, 1006, 1404, 2803
TaiI ACGT 4 cut(s) 567, 1733, 1777, 2746
TaqI TCGA 7 cut(s) 155, 161, 225, 600, 937, 1021, 1484
TatI WGTACW 2 cut(s) 91, 926
TauI GCSGC 3 cut(s) 11, 14, 171
TfiI GAWTC 8 cut(s) 638, 722, 781, 893, 1517, 2382, 2816, 2834
Tru1I TTAA 7 cut(s) 1554, 1662, 2163, 2549, 2586, 2958, 2970
Tru9I TTAA 7 cut(s) 1554, 1662, 2163, 2549, 2586, 2958, 2970
TscAI CASTG 8 cut(s) 304, 397, 595, 1011, 1075, 1317, 1344, 1459
TseFI GTSAC 3 cut(s) 299, 584, 2600
TseI GCWGC 6 cut(s) 185, 289, 292, 793, 1960, 2111
Tsp45I GTSAC 3 cut(s) 299, 584, 2600
TspGWI ACGGA 2 cut(s) 238, 391
TspRI CASTG 8 cut(s) 304, 397, 595, 1011, 1075, 1317, 1344, 1459
Tth111I GACNNNGTC 1 cut(s) 1729
Vha464I CTTAAG 2 cut(s) 2162, 2548
VneI GTGCAC 1 cut(s) 2666
VpaK11BI GGWCC 2 cut(s) 307, 2617
XagI CCTNNNNNAGG 1 cut(s) 1677
XapI RAATTY 7 cut(s) 77, 1140, 1261, 2050, 2099, 2194, 2485
XbaI TCTAGA 1 cut(s) 2378
XceI RCATGY 4 cut(s) 697, 1127, 1713, 3030
XmiI GTMKAC 1 cut(s) 1897
XspI CTAG 6 cut(s) 746, 878, 1460, 2246, 2379, 2931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.