Rw4G011060

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005499
Physical Location & Seq
Forward (+)
26198125 .. 26213228
15104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011060.1

Sequence Viewer

Length: 3453 bp
ATGAGAGCGGCGGCGTCTCTCCTTGTCAAATCAGTAGCCTCCTCCAACAATACTTGTTTTCGTTCTCACCACCTCAAATTCTGTCTTGTTGTTCTCCAACCACAATCACAACCACACTCTTCTTCTTCTTCCTCTTCTACCATTTCGATTTCGAAGCGCGGCTTTTCCGGCTACGCTGCGGAGCAGTTCTCCGACGACGAGTATGAGTGCGATTTCGAAAGCCAAAAGGCTTCATCTTCCGTGGCCAACATTGACGAGTGGAAATGGAAACTGAGCCTGCTTCTGCGCAGTGACAAGGACCAAGAGGTTGTTTCCAGAGATAAAAGGGATAGGAGAGACTATGAGCAAATATCTAACCTCGCCAAACGGATGGGACTTTACACTGAAATATATGGCAAAGTGGTGGTTGCCAGCAAGGTTCCTCTTCCAAACTACCGCCCCGATTTGGATGATAAGCGCCCACAAAGAGAGGTGGTTATCCCTCTTGGCTTGCAAAGGAGGGTAGAAGGTTTACTGCAAGAACACCTTGATCGTGTCCTGCTAAGTTCCCGCAACGTATCAGCCAATTCAGGTGGTGACAGTGACCACTTCGATCAGGTTGGAAATGAAAAGCCTGATGCGAATGCAGATTCTTTTCTTGATGGGTCTGTAATGGAGAAGGTTCTTCAGCGTAGGAGTTTGCGCATGCGTAATATGCAAAGAGCTTGGCAGGAATCACCTGAAGGTAAAAAGATGCTAGAGTTCCGGAAATCACTACCATCTTTCAAAGAGAATCAGAGGTTGCTGCAAGCAATAGCACAAAATCAGGTAGTAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAGCTTCCTCAATATATACTAGAGTCAGAGATAGAATCTGGCCGTGGAGCATTCTGTAGCATTATCTGTACACAACCTCGAAGAATATCTGCTATGGCTGTTGCTGAAAGAGTGTCTGCAGAGAGGGGAGAGCTTCTTGGTGAAACAGTGGGCTATAAAGTTCGATTAGAGGGAATGAAAGGAAAAAATACGCATCTCCTTTTCTGTACCAGTGGTATTCTACTTCGCCGTCTGTTGAGTGATCGGAACTTGAGTGGTGTAACACATGTTTTTGTTGATGAAATTCATGAACGAGGCATGAATGAAGACTTCTTGTTGATTGTATTGAAAGATCTGCTTCCACGCCGTCGGGATCTAAGATTGGTTTTGATGAGTGCCACTCTGAATGCGGAGCTATTTTCAAATTTTTTTGGAGGAGCACCAACAATTCACATTCCGGGTTTCACATATCCAGTGAGAGCACTCTTTTTGGAAGATGTACTGGAAATGACTGGTTATAAGCTGACTTCTTTCAACCAAATTGATGACTATGGCCAAGACAAACTGTGGAAAACGCAGAAGCAGTTAGCGCCTCGGAAAAGGAAAAACCAGATCACTGCTCTAGTTGAGGATGCTCTTAGTAAATCGAGTTTTGAGAATTATAGCCCCAGAGCACGCGATTCATTATCATGTTGGACGGCTGATTGCATAGGATTTAATCTTATTGAAGCAGTTTTATGTCATATATGTCGGAAGGAACGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGAGGATATCAGCTGTTTGAGGGATCAACTTAAAGCCCATCCTCTTTTAGGTGATCCTAACAGGGTTTTGCTTATAACATGCCATGGGTCAATGACCACGTCTGAACAGAACCTCATTTTCGCGAGACCGCCTCCTAATGTACGTAAAATTGTTCTTGCTACTAATATGGCAGAAGCAAGCATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGGAAAGCAAAAGAGACCACATATGATGCTTTGAATAATACACCTTGTCTACTACCTTCTTGGATATCCCAAGCATCTGCTCGGCAAAGAAGGGGAAGGGCTGGCCGGGTGCAGCCAGGCGAATGTTACCACCTTTACCCCAAATGTGTGTATCATGCGTTTGCAGAATACCAACTTCCTGAACTCCTAAGGACTCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTTTGCAGGTTGGTAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCACCTGAACCCCTTGCTGTTCAAAATGCTGTTGGCTTTCTTAAGTTGATTGGGGCATTGGATGAGAATGAAATTTTGACAAATCTTGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTAAGTGTCCGGGATCCATTCCTTTTGCCTCAAGACAAAAAAGATTTAGCGGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTATAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCAGAAAGAGAAGGATCAGCATATGAATACTGCTGGAGAAATTTTCTCTCTGCCCAAACACTTCAAGCTATTCATTCTCTTCGGAAGCAGTTTAGCTACATCTTAAGAGACGCTGGCTTGGTGGATGCAGATGCAAGCATTAACAACAAGCTAAGTCACAATCAGTCATTGGTCCGCGCAATCATATGTTCCGGACTATTCCCTGGCATAGCATCTGTAGTGCACCGAGAGACATCCATGTCTTTCAAAACTATGGATGATGGCCAGGTTTTACTATATGCTAATTCTGTAAATGCACGTTACCAAACAATTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAGTAAAGGTCAACACAGTTTTCATACGCGATTCTACTGGTGTCTCTGATTCCATTTTGATCCTTTTTGGTGGTGCCCTAAACCATGGTATCCAGGCTGGGCATTTGAAAATGTTGGACGGCTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGCTTAAAGAAGAGCTTAATGAGCTTATTCAGAAGAAGCTTCAAGATCCAAGCCTCGATATCCACAAGGAAGGAAAGTATCTCATACTTGCTGTTCAAGAGCTGGTATCTGGAGATCAAAGTGAAGGAAGATTTGTGTTTGGCCGTGAAAACAGGAAGCCAAAGGAGTCCTGTGATAACAGCAGATTCACAAGGGATGGAACCAATCCTAAGAGTTTGCTACAAACTCTATTGATGAGAGCAGGACATTCACCTCCAAAATACAAAACCAAGCACCTCAAGACAAATGAGTTCAGAGCACTTGTGGAGTTCAAGGGCATGCAATTTGTTGGGAAGCCCAAGAAAAATAAGCAGCTTGCCGAAAGGGATGCTGCCATAGAAGCACTGGCCTGGTTGACTCACACCTCAGACAACAGTCGTGATGAAGATGACAACTCCCCACCTGATGTCACTAACAACATGCTGAAACTTCTGGGGAAACGTAGAAGATCAAAGCGCCACTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1150

Amino Acids

129.23

Weight (kDa)

8.84

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 260 - 414 2.5e-09 DEAD/DEAH box helicase
Helicase_C PF00271 521 - 650 3.6e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 714 - 742 1.8e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 743 - 803 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 870 - 951 5.4e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 960 - 1018 2.6e-23 C-terminal domain in RNA helicases
dsrm PF00035 1051 - 1113 7.7e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1347, 1697
AasI GACNNNNNNGTC 1 cut(s) 2674
Acc16I TGCGCA 2 cut(s) 287, 683
Acc36I ACCTGC 1 cut(s) 2062
AccB1I GGYRCC 1 cut(s) 2849
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 1888
AccII CGCG 5 cut(s) 159, 1506, 1745, 2613, 2805
AccIII TCCGGA 2 cut(s) 744, 2627
AcoI YGGCCR 7 cut(s) 243, 889, 1381, 1589, 1942, 2698, 3085
AcsI RAATTY 7 cut(s) 77, 1131, 1252, 2041, 2090, 2184, 2476
AcuI CTGAAG 2 cut(s) 650, 741
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 4 cut(s) 919, 1057, 1329, 1764
AfiI CCNNNNNNNGG 6 cut(s) 445, 1670, 2237, 2238, 2757, 2761
AflII CTTAAG 2 cut(s) 2153, 2539
AflIII ACRYGT 1 cut(s) 1114
AjiI CACGTC 1 cut(s) 1722
AjnI CCWGG 6 cut(s) 1591, 1954, 2638, 2700, 2868, 3332
Alw21I GWGCWC 5 cut(s) 1270, 1312, 1504, 2661, 3244
Alw26I GTCTC 8 cut(s) 21, 330, 821, 1741, 1847, 2538, 2660, 2824
Alw44I GTGCAC 1 cut(s) 2657
AlwNI CAGNNNCTG 1 cut(s) 1598
Aor13HI TCCGGA 2 cut(s) 744, 2627
AoxI GGCC 8 cut(s) 243, 889, 1381, 1589, 1942, 2698, 3085, 3330
ApaLI GTGCAC 1 cut(s) 2657
ApeKI GCWGC 6 cut(s) 176, 784, 1951, 2102, 3295, 3314
ApoI RAATTY 7 cut(s) 77, 1131, 1252, 2041, 2090, 2184, 2476
AspLEI GCGC 7 cut(s) 159, 288, 459, 684, 1420, 2615, 3441
AspS9I GGNCC 2 cut(s) 298, 2608
AsuC2I CCSGG 3 cut(s) 1287, 1946, 2315
AsuHPI GGTGA 7 cut(s) 59, 587, 708, 1001, 1685, 2783, 3186
AsuII TTCGAA 2 cut(s) 152, 216
AvaII GGWCC 2 cut(s) 298, 2608
AxyI CCTNAGG 1 cut(s) 2027
BaeGI GKGCMC 2 cut(s) 2661, 2854
BaeI ACNNNNGTAYC 2 cut(s) 2848, 2881
BalI TGGCCA 3 cut(s) 245, 1383, 2700
BamHI GGATCC 2 cut(s) 2317, 2917
BanI GGYRCC 1 cut(s) 2849
BbsI GAAGAC 1 cut(s) 1161
Bbv12I GWGCWC 5 cut(s) 1270, 1312, 1504, 2661, 3244
BbvI GCAGC 6 cut(s) 163, 771, 1963, 2089, 3301, 3307
BccI CCATC 8 cut(s) 364, 635, 766, 1668, 2421, 2690, 2923, 3134
BceAI ACGGC 7 cut(s) 876, 1062, 1179, 1542, 1604, 2911, 3072
BcgI CGANNNNNNTGC 2 cut(s) 3293, 3327
BciT130I CCWGG 6 cut(s) 1593, 1956, 2640, 2702, 2870, 3334
BciVI GTATCC 1 cut(s) 2876
BclI TGATCA 1 cut(s) 2395
BcnI CCSGG 3 cut(s) 1287, 1946, 2315
BcoDI GTCTC 8 cut(s) 21, 330, 821, 1741, 1847, 2538, 2660, 2824
BfaI CTAG 6 cut(s) 737, 869, 1451, 2237, 2370, 2922
BfmI CTRYAG 3 cut(s) 904, 966, 2652
BfoI RGCGCY 3 cut(s) 460, 1421, 3442
BfrI CTTAAG 2 cut(s) 2153, 2539
BfuAI ACCTGC 1 cut(s) 2062
BfuI GTATCC 1 cut(s) 2876
BglII AGATCT 1 cut(s) 1180
BisI GCNGC 9 cut(s) 9, 12, 160, 177, 785, 1952, 2103, 3296, 3315
BlsI GCNGC 9 cut(s) 10, 13, 161, 178, 786, 1953, 2104, 3297, 3316
Bme1390I CCNGG 9 cut(s) 1287, 1593, 1946, 1956, 2315, 2640, 2702, 2870, 3334
Bme18I GGWCC 2 cut(s) 298, 2608
BmgBI CACGTC 1 cut(s) 1722
BmgT120I GGNCC 2 cut(s) 298, 2608
BmiI GGNNCC 5 cut(s) 420, 2319, 2851, 2919, 3145
BmrFI CCNGG 9 cut(s) 1287, 1593, 1946, 1956, 2315, 2640, 2702, 2870, 3334
BmrI ACTGGG 1 cut(s) 1626
BmuI ACTGGG 1 cut(s) 1626
BoxI GACNNNNGTC 1 cut(s) 3357
BpiI GAAGAC 1 cut(s) 1161
BplI GAGNNNNNCTC 6 cut(s) 173, 205, 1213, 1245, 1738, 1770
BpmI CTGGAG 2 cut(s) 2491, 3075
Bpu14I TTCGAA 2 cut(s) 152, 216
BpuEI CTTGAG 3 cut(s) 1120, 2319, 3206
BpuMI CCSGG 3 cut(s) 1287, 1946, 2315
BsaAI YACGTR 1 cut(s) 1766
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 2 cut(s) 1741, 1847
BsaJI CCNNGG 7 cut(s) 240, 892, 1421, 1705, 2638, 2756, 2860
BsaWI WCCGGW 2 cut(s) 744, 2627
Bsc4I CCNNNNNNNGG 6 cut(s) 445, 1670, 2237, 2238, 2757, 2761
Bse1I ACTGG 9 cut(s) 835, 1059, 1301, 1335, 1345, 1621, 2222, 2818, 3333
Bse21I CCTNAGG 1 cut(s) 2027
Bse3DI GCAATG 1 cut(s) 2269
BseAI TCCGGA 2 cut(s) 744, 2627
BseBI CCWGG 6 cut(s) 1593, 1956, 2640, 2702, 2870, 3334
BseDI CCNNGG 7 cut(s) 240, 892, 1421, 1705, 2638, 2756, 2860
BseLI CCNNNNNNNGG 6 cut(s) 445, 1670, 2237, 2238, 2757, 2761
BseMI GCAATG 1 cut(s) 2269
BseMII CTCAG 3 cut(s) 263, 863, 3363
BseNI ACTGG 9 cut(s) 835, 1059, 1301, 1335, 1345, 1621, 2222, 2818, 3333
BseRI GAGGAG 2 cut(s) 31, 1278
BseSI GKGCMC 2 cut(s) 2661, 2854
BseXI GCAGC 6 cut(s) 163, 771, 1963, 2089, 3301, 3307
BseYI CCCAGC 1 cut(s) 2873
BsgI GTGCAG 1 cut(s) 1970
Bsh1236I CGCG 5 cut(s) 159, 1506, 1745, 2613, 2805
BshFI GGCC 8 cut(s) 245, 891, 1383, 1591, 1944, 2700, 3087, 3332
BshNI GGYRCC 1 cut(s) 2849
BsiHKAI GWGCWC 5 cut(s) 1270, 1312, 1504, 2661, 3244
BsiSI CCGG 6 cut(s) 168, 745, 1286, 1945, 2314, 2628
BslFI GGGAC 2 cut(s) 387, 2371
BslI CCNNNNNNNGG 6 cut(s) 445, 1670, 2237, 2238, 2757, 2761
BsmAI GTCTC 8 cut(s) 21, 330, 821, 1741, 1847, 2538, 2660, 2824
BsmBI CGTCTC 2 cut(s) 21, 2538
BsmFI GGGAC 2 cut(s) 387, 2371
BsmI GAATGC 3 cut(s) 628, 899, 1240
BsnI GGCC 8 cut(s) 245, 891, 1383, 1591, 1944, 2700, 3087, 3332
Bso31I GGTCTC 2 cut(s) 1741, 1847
Bsp119I TTCGAA 2 cut(s) 152, 216
Bsp1286I GDGCHC 6 cut(s) 1270, 1312, 1504, 2661, 2854, 3244
Bsp13I TCCGGA 2 cut(s) 744, 2627
Bsp1407I TGTACA 1 cut(s) 917
Bsp19I CCATGG 2 cut(s) 1705, 2860
Bsp68I TCGCGA 1 cut(s) 1745
BspANI GGCC 8 cut(s) 245, 891, 1383, 1591, 1944, 2700, 3087, 3332
BspCNI CTCAG 3 cut(s) 264, 862, 3362
BspEI TCCGGA 2 cut(s) 744, 2627
BspFNI CGCG 5 cut(s) 159, 1506, 1745, 2613, 2805
BspHI TCATGA 2 cut(s) 1135, 1611
BspLI GGNNCC 5 cut(s) 420, 2319, 2851, 2919, 3145
BspMAI CTGCAG 1 cut(s) 970
BspMI ACCTGC 1 cut(s) 2062
BspQI GCTCTTC 1 cut(s) 2949
BspT104I TTCGAA 2 cut(s) 152, 216
BspT107I GGYRCC 1 cut(s) 2849
BspTI CTTAAG 2 cut(s) 2153, 2539
BspTNI GGTCTC 2 cut(s) 1741, 1847
BsrBI CCGCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 2269
BsrGI TGTACA 1 cut(s) 917
BsrI ACTGG 9 cut(s) 835, 1059, 1301, 1335, 1345, 1621, 2222, 2818, 3333
BssECI CCNNGG 7 cut(s) 240, 892, 1421, 1705, 2638, 2756, 2860
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 3 cut(s) 1705, 2756, 2860
Bst2UI CCWGG 6 cut(s) 1593, 1956, 2640, 2702, 2870, 3334
Bst4CI ACNGT 5 cut(s) 581, 997, 1395, 2794, 3359
Bst6I CTCTTC 5 cut(s) 124, 139, 429, 2520, 2949
BstACI GRCGYC 1 cut(s) 14
BstAFI CTTAAG 2 cut(s) 2153, 2539
BstAUI TGTACA 1 cut(s) 917
BstBAI YACGTR 1 cut(s) 1766
BstBI TTCGAA 2 cut(s) 152, 216
BstDSI CCRYGG 4 cut(s) 240, 892, 1705, 2860
BstENI CCTNNNNNAGG 1 cut(s) 1668
BstFNI CGCG 5 cut(s) 159, 1506, 1745, 2613, 2805
BstH2I RGCGCY 3 cut(s) 460, 1421, 3442
BstHHI GCGC 7 cut(s) 159, 288, 459, 684, 1420, 2615, 3441
BstMAI GTCTC 8 cut(s) 21, 330, 821, 1741, 1847, 2538, 2660, 2824
BstMWI GCNNNNNNNGC 6 cut(s) 168, 694, 1417, 2360, 2965, 3323
BstNI CCWGG 6 cut(s) 1593, 1956, 2640, 2702, 2870, 3334
BstNSI RCATGY 5 cut(s) 688, 1118, 1704, 3265, 3406
BstPAI GACNNNNGTC 1 cut(s) 3357
BstSCI CCNGG 9 cut(s) 1285, 1591, 1944, 1954, 2313, 2638, 2700, 2868, 3332
BstSFI CTRYAG 3 cut(s) 904, 966, 2652
BstSLI GKGCMC 2 cut(s) 2661, 2854
BstSNI TACGTA 1 cut(s) 1766
BstUI CGCG 5 cut(s) 159, 1506, 1745, 2613, 2805
BstV1I GCAGC 6 cut(s) 163, 771, 1963, 2089, 3301, 3307
BstV2I GAAGAC 1 cut(s) 1161
BstX2I RGATCY 6 cut(s) 1180, 1201, 2227, 2317, 2917, 2989
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 6 cut(s) 1180, 1201, 2227, 2317, 2917, 2989
Bsu36I CCTNAGG 1 cut(s) 2027
BsuI GTATCC 1 cut(s) 2876
BsuRI GGCC 8 cut(s) 245, 891, 1383, 1591, 1944, 2700, 3087, 3332
BtgI CCRYGG 4 cut(s) 240, 892, 1705, 2860
BtrI CACGTC 1 cut(s) 1722
BtsI GCAGTG 2 cut(s) 295, 1443
BtsIMutI CAGTG 8 cut(s) 295, 381, 586, 1002, 1066, 1308, 1443, 3326
BtuMI TCGCGA 1 cut(s) 1745
BveI ACCTGC 1 cut(s) 2062
CaiI CAGNNNCTG 1 cut(s) 1598
CciI TCATGA 2 cut(s) 1135, 1611
CfoI GCGC 7 cut(s) 159, 288, 459, 684, 1420, 2615, 3441
Cfr13I GGNCC 2 cut(s) 298, 2608
CseI GACGC 2 cut(s) 3, 2555
Csp6I GTAC 4 cut(s) 918, 1056, 1328, 1763
CspCI CAANNNNNGTGG 2 cut(s) 553, 588
CviQI GTAC 4 cut(s) 918, 1056, 1328, 1763
DrdI GACNNNNNNGTC 1 cut(s) 2674
DseDI GACNNNNNNGTC 1 cut(s) 2674
EaeI YGGCCR 7 cut(s) 243, 889, 1381, 1589, 1942, 2698, 3085
Eam1104I CTCTTC 5 cut(s) 124, 139, 429, 2520, 2949
EarI CTCTTC 5 cut(s) 124, 139, 429, 2520, 2949
Eco105I TACGTA 1 cut(s) 1766
Eco130I CCWWGG 3 cut(s) 1705, 2756, 2860
Eco31I GGTCTC 2 cut(s) 1741, 1847
Eco32I GATATC 4 cut(s) 819, 1630, 1905, 3004
Eco47I GGWCC 2 cut(s) 298, 2608
Eco57I CTGAAG 2 cut(s) 650, 741
Eco81I CCTNAGG 1 cut(s) 2027
EcoNI CCTNNNNNAGG 1 cut(s) 1668
EcoRI GAATTC 2 cut(s) 2041, 2090
EcoRII CCWGG 6 cut(s) 1591, 1954, 2638, 2700, 2868, 3332
EcoRV GATATC 4 cut(s) 819, 1630, 1905, 3004
EcoT14I CCWWGG 3 cut(s) 1705, 2756, 2860
ErhI CCWWGG 3 cut(s) 1705, 2756, 2860
Esp3I CGTCTC 2 cut(s) 21, 2538
FaqI GGGAC 2 cut(s) 387, 2371
FauI CCCGC 2 cut(s) 557, 2347
FauNDI CATATG 5 cut(s) 1861, 2400, 2419, 2458, 2621
FbaI TGATCA 1 cut(s) 2395
FblI GTMKAC 1 cut(s) 1888
Fnu4HI GCNGC 9 cut(s) 9, 12, 160, 177, 785, 1952, 2103, 3296, 3315
Fsp4HI GCNGC 9 cut(s) 9, 12, 160, 177, 785, 1952, 2103, 3296, 3315
FspBI CTAG 6 cut(s) 737, 869, 1451, 2237, 2370, 2922
FspI TGCGCA 2 cut(s) 287, 683
GlaI GCGC 7 cut(s) 158, 287, 458, 683, 1419, 2614, 3440
GluI GCNGC 9 cut(s) 9, 12, 160, 177, 785, 1952, 2103, 3296, 3315
GsaI CCCAGC 1 cut(s) 2877
GsuI CTGGAG 2 cut(s) 2491, 3075
HaeII RGCGCY 3 cut(s) 460, 1421, 3442
HaeIII GGCC 8 cut(s) 245, 891, 1383, 1591, 1944, 2700, 3087, 3332
HapII CCGG 6 cut(s) 168, 745, 1286, 1945, 2314, 2628
HgaI GACGC 2 cut(s) 3, 2555
HhaI GCGC 7 cut(s) 159, 288, 459, 684, 1420, 2615, 3441
Hin1I GRCGYC 1 cut(s) 14
Hin6I GCGC 7 cut(s) 157, 286, 457, 682, 1418, 2613, 3439
HinP1I GCGC 7 cut(s) 157, 286, 457, 682, 1418, 2613, 3439
HincII GTYRAC 2 cut(s) 2788, 3339
HindII GTYRAC 2 cut(s) 2788, 3339
HindIII AAGCTT 2 cut(s) 2945, 2981
HpaII CCGG 6 cut(s) 168, 745, 1286, 1945, 2314, 2628
HphI GGTGA 7 cut(s) 59, 587, 708, 1001, 1685, 2783, 3186
Hpy166II GTNNAC 6 cut(s) 512, 920, 1889, 2659, 2788, 3339
Hpy8I GTNNAC 6 cut(s) 512, 920, 1889, 2659, 2788, 3339
Hpy99I CGWCG 3 cut(s) 197, 200, 1200
HpyCH4III ACNGT 5 cut(s) 581, 997, 1395, 2794, 3359
HpyCH4IV ACGT 5 cut(s) 555, 1721, 1765, 2734, 3424
HpyF10VI GCNNNNNNNGC 6 cut(s) 168, 694, 1417, 2360, 2965, 3323
HpySE526I ACGT 5 cut(s) 555, 1721, 1765, 2734, 3424
Hsp92I GRCGYC 1 cut(s) 14
HspAI GCGC 7 cut(s) 157, 286, 457, 682, 1418, 2613, 3439
Kpn2I TCCGGA 2 cut(s) 744, 2627
Ksp22I TGATCA 1 cut(s) 2395
LguI GCTCTTC 1 cut(s) 2949
LmnI GCTCC 4 cut(s) 181, 896, 1240, 1265
Lsp1109I GCAGC 6 cut(s) 163, 771, 1963, 2089, 3301, 3307
MaeI CTAG 6 cut(s) 737, 869, 1451, 2237, 2370, 2922
MaeII ACGT 5 cut(s) 555, 1721, 1765, 2734, 3424
MaeIII GTNAC 8 cut(s) 290, 575, 581, 1108, 1964, 2591, 2735, 3391
MbiI CCGCTC 1 cut(s) 8
MflI RGATCY 6 cut(s) 1180, 1201, 2227, 2317, 2917, 2989
MhlI GDGCHC 6 cut(s) 1270, 1312, 1504, 2661, 2854, 3244
MlsI TGGCCA 3 cut(s) 245, 1383, 2700
MluNI TGGCCA 3 cut(s) 245, 1383, 2700
MlyI GAGTC 4 cut(s) 881, 2026, 3119, 3334
MmeI TCCRAC 7 cut(s) 69, 121, 216, 580, 1502, 1559, 2871
Mox20I TGGCCA 3 cut(s) 245, 1383, 2700
MroI TCCGGA 2 cut(s) 744, 2627
MscI TGGCCA 3 cut(s) 245, 1383, 2700
MseI TTAA 8 cut(s) 1545, 1653, 2154, 2540, 2577, 2949, 2961, 3451
MslI CAYNNNNRTG 1 cut(s) 1516
Msp20I TGGCCA 3 cut(s) 245, 1383, 2700
MspA1I CMGCKG 2 cut(s) 1635, 2102
MspCI CTTAAG 2 cut(s) 2153, 2539
MspI CCGG 6 cut(s) 168, 745, 1286, 1945, 2314, 2628
MspR9I CCNGG 9 cut(s) 1287, 1593, 1946, 1956, 2315, 2640, 2702, 2870, 3334
Mva1269I GAATGC 3 cut(s) 628, 899, 1240
MvaI CCWGG 6 cut(s) 1593, 1956, 2640, 2702, 2870, 3334
MvnI CGCG 5 cut(s) 159, 1506, 1745, 2613, 2805
MwoI GCNNNNNNNGC 6 cut(s) 168, 694, 1417, 2360, 2965, 3323
NciI CCSGG 3 cut(s) 1287, 1946, 2315
NcoI CCATGG 2 cut(s) 1705, 2860
NdeI CATATG 5 cut(s) 1861, 2400, 2419, 2458, 2621
NlaIV GGNNCC 5 cut(s) 420, 2319, 2851, 2919, 3145
NmeAIII GCCGAG 1 cut(s) 1900
NmuCI GTSAC 5 cut(s) 290, 575, 581, 2591, 3391
NruI TCGCGA 1 cut(s) 1745
NsbI TGCGCA 2 cut(s) 287, 683
NspI RCATGY 5 cut(s) 688, 1118, 1704, 3265, 3406
NspV TTCGAA 2 cut(s) 152, 216
PaeI GCATGC 2 cut(s) 688, 3265
PagI TCATGA 2 cut(s) 1135, 1611
PciI ACATGT 1 cut(s) 1114
PciSI GCTCTTC 1 cut(s) 2949
PctI GAATGC 3 cut(s) 628, 899, 1240
PfeI GAWTC 9 cut(s) 629, 713, 772, 884, 1508, 2373, 2807, 2825, 3129
PflFI GACNNNGTC 1 cut(s) 1720
PfoI TCCNGGA 1 cut(s) 2313
PkrI GCNGC 9 cut(s) 10, 13, 161, 178, 786, 1953, 2104, 3297, 3316
PleI GAGTC 4 cut(s) 880, 2026, 3118, 3334
PpsI GAGTC 4 cut(s) 880, 2026, 3118, 3334
Ppu21I YACGTR 1 cut(s) 1766
PscI ACATGT 1 cut(s) 1114
PshAI GACNNNNGTC 1 cut(s) 3357
PsiI TTATAA 2 cut(s) 1347, 1697
Psp6I CCWGG 6 cut(s) 1591, 1954, 2638, 2700, 2868, 3332
PspFI CCCAGC 1 cut(s) 2873
PspGI CCWGG 6 cut(s) 1591, 1954, 2638, 2700, 2868, 3332
PspN4I GGNNCC 5 cut(s) 420, 2319, 2851, 2919, 3145
PspPI GGNCC 2 cut(s) 298, 2608
PstI CTGCAG 1 cut(s) 970
PstNI CAGNNNCTG 1 cut(s) 1598
PsuI RGATCY 6 cut(s) 1180, 1201, 2227, 2317, 2917, 2989
PsyI GACNNNGTC 1 cut(s) 1720
PvuII CAGCTG 2 cut(s) 1635, 2102
RruI TCGCGA 1 cut(s) 1745
RsaI GTAC 4 cut(s) 919, 1057, 1329, 1764
RsaNI GTAC 4 cut(s) 918, 1056, 1328, 1763
RseI CAYNNNNRTG 1 cut(s) 1516
SapI GCTCTTC 1 cut(s) 2949
SaqAI TTAA 8 cut(s) 1545, 1653, 2154, 2540, 2577, 2949, 2961, 3451
SatI GCNGC 9 cut(s) 9, 12, 160, 177, 785, 1952, 2103, 3296, 3315
Sau96I GGNCC 2 cut(s) 298, 2608
SchI GAGTC 4 cut(s) 881, 2026, 3119, 3334
ScrFI CCNGG 9 cut(s) 1287, 1593, 1946, 1956, 2315, 2640, 2702, 2870, 3334
SduI GDGCHC 6 cut(s) 1270, 1312, 1504, 2661, 2854, 3244
SfcI CTRYAG 3 cut(s) 904, 966, 2652
SfuI TTCGAA 2 cut(s) 152, 216
SinI GGWCC 2 cut(s) 298, 2608
SmiMI CAYNNNNRTG 1 cut(s) 1516
SmlI CTYRAG 5 cut(s) 1099, 2153, 2334, 2539, 3221
SmoI CTYRAG 5 cut(s) 1099, 2153, 2334, 2539, 3221
SnaBI TACGTA 1 cut(s) 1766
SphI GCATGC 2 cut(s) 688, 3265
SspI AATATT 1 cut(s) 2207
SspMI CTAG 6 cut(s) 737, 869, 1451, 2237, 2370, 2922
StyD4I CCNGG 9 cut(s) 1285, 1591, 1944, 1954, 2313, 2638, 2700, 2868, 3332
StyI CCWWGG 3 cut(s) 1705, 2756, 2860
TaaI ACNGT 5 cut(s) 581, 997, 1395, 2794, 3359
TaiI ACGT 5 cut(s) 558, 1724, 1768, 2737, 3427
TaqI TCGA 8 cut(s) 146, 152, 216, 591, 928, 1012, 1475, 3000
TatI WGTACW 2 cut(s) 917, 1327
TauI GCSGC 3 cut(s) 11, 14, 162
TfiI GAWTC 9 cut(s) 629, 713, 772, 884, 1508, 2373, 2807, 2825, 3129
Tru1I TTAA 8 cut(s) 1545, 1653, 2154, 2540, 2577, 2949, 2961, 3451
Tru9I TTAA 8 cut(s) 1545, 1653, 2154, 2540, 2577, 2949, 2961, 3451
TscAI CASTG 8 cut(s) 295, 388, 586, 1002, 1066, 1308, 1450, 3333
TseFI GTSAC 5 cut(s) 290, 575, 581, 2591, 3391
TseI GCWGC 6 cut(s) 176, 784, 1951, 2102, 3295, 3314
Tsp45I GTSAC 5 cut(s) 290, 575, 581, 2591, 3391
TspGWI ACGGA 2 cut(s) 229, 382
TspRI CASTG 8 cut(s) 295, 388, 586, 1002, 1066, 1308, 1450, 3333
Tth111I GACNNNGTC 1 cut(s) 1720
Vha464I CTTAAG 2 cut(s) 2153, 2539
VneI GTGCAC 1 cut(s) 2657
VpaK11BI GGWCC 2 cut(s) 298, 2608
XagI CCTNNNNNAGG 1 cut(s) 1668
XapI RAATTY 7 cut(s) 77, 1131, 1252, 2041, 2090, 2184, 2476
XbaI TCTAGA 1 cut(s) 2369
XceI RCATGY 5 cut(s) 688, 1118, 1704, 3265, 3406
XcmI CCANNNNNNNNNTGG 1 cut(s) 3325
XmiI GTMKAC 1 cut(s) 1888
XspI CTAG 6 cut(s) 737, 869, 1451, 2237, 2370, 2922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.