RchiOBHm_Chr4g0406081

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005499
Physical Location & Seq
Forward (+)
27110298 .. 27124943
14646 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37751

Sequence Viewer

Length: 3447 bp
ATGAGAGCGGCGGCGTCTCTCCTTGTCAAATCAGTAGCCTCTTCCAACAATGCTTGTTTTCGTTCTCACCACCTCAAATTCTGTCTAGTTGTACTCCAACCACAATCACAACCACACTCTTCTTCTTCTTCTCCCATTTCGATTTCGAAGCGCGGATTTTCCGGCTACGCTGCGGAGCAGTTCTCCGACGACGAGTATGAGTGCGATTTCGAAAGCCAAAAGGCTTCATCTTCCGTGGCCAACATTGACGAGTGGAAATGGAAACTGAGCCTGCTGCTGCGCAGTGACAAGGACCAAGAGATTGTTTCCAGAGATAAAAGGGATAGGAGAGACTACGAGCAAATATCTAACCTCGCCAAACGGATGGGACTTTACACTGAAATATATGGCAAAGTGGTGGTTGCCAGCAAGGTTCCTCTTCCAAACTACCGCCCCGATTTGGATGATAAGCGCCCACAAAGAGAGGTGGTTATCCCTCTTGGCTTGCAAAGGAGGGTAGAGGGTTTACTGCAAGAACACCTTGATCGTGTCCTGCTAAGTTTCCGCAACGTATCAGCCAATTCAGGTGGTGACAGTGAGCACTTCGGTCAGGTTGAAAATGATAAGCCTGATGAGAATGCAGATTCTCTTCTTGATGGGTCTGTAATGGAGAAGGTTCTTCAGCGTAGGAGTTTGCGCATGCGTAATATGCAAAGAGCTTGGCAGGAATCACCTGAAGGTAAAAAGATGCTAGAGTTCCGGAAATCACTACCATCTTTCAAAGAGAATCAGAGGTTGCTGCAAGCAATAGCACAAAATCAGGTAGTAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAGCTTCCTCAATATATACTAGAGTCAGAGATAGAATCTGGCCGTGGAGCATTCTGTAGCATTATCTGTACACAACCTCGAAGAATATCTGCTATGGCTGTTGCTGAAAGAGTGTCTGCAGAGAGGGGAGAGCCTCTTGGTGAAACAGTGGGCTATAAAGTTCGATTAGAGGGAATGAAAGGAAAAAATACGCATCTCCTTTTCTGTACCAGTGGTATTCTACTTCGCCGTCTGTTGAGTGATCGGAACTTGAGTGGTGTAACACATGTTTTTGTTGATGAAATTCATGAACGAGGCATGAATGAAGACTTCTTGTTGATTGTATTGAAAGATCTGCTTCCACGCCGTCGGGATCTAAGATTGGTTTTGATGAGTGCCACTCTGAATGCGGAGCTATTTTCAAATTTTTTTGGAGGAGCACCAACAATTCACATTCCGGGTTTCACATATCCAGTGAAAGCACACTTTTTGGAAGATGTACTGGAAATGACTGGGTATAAGCTGACTTCTTTCAACCAAATTGATGACTATGGCCAAGACAAACTGTGGAAAACGCAGAAGCAGTTAGCGCCTCGGAAAAGGAAAAATCAGATCACTGCTCTAGTTGAGGATGCTCTTAGTAAATCGAGTTTTGAGAATTATAGCCCCAGAGCACGCGATTCATTATCATGTTGGACGGCTGATTGCATAGGATTTAATCTTATTGAAGCAGTTTTATGTCATATATGTCGGAAGGAACGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGAGGATATCAGCTGTTTGAGGGATCAACTTAAAGCCCATCCTCTTTTAGGTGATCCTAACAGGGTTTTGCTTATCACATGCCATGGGTCAATGACCACGTCTGAACAGAACCTCATTTTCGCGAGACCGCCTCCTAATGTACGTAAAATTGTTCTTGCTACTAATATGGCAGAAGCAAGCATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCACATATGATGCTTTGAATAATACACCTTGTCTACTACCTTCTTGGATATCCCAAGCATCTGCTCGGCAAAGAAGGGGAAGGGCTGGCCGGGTGCAGCCAGGCGAATGTTACCACCTTTACCCCAAATGTGTGTATCATGCGTTTGCAGAATACCAACTTCCTGAACTCCTAAGGACTCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTTTGCAGGTCGGTAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCGCCTGAACCCCTTGCTGTTCAAAATGCTGTTGGCTTTCTCAAGTTGATTGGGGCATTGGATGAGAATGAAAATTTGACAAATCTCGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTAAGTGTCCGGGATCCATTCCTTTTGCCTCAAGACAAAAAAGATTTAGCGGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTATAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCAGAAAGAGAAGGATCAGCATATGAATACTGCTGGAGAAATTTTCTCTCTGCCCAGACACTTCAAGCTATTCATTCTCTTCGTAAGCAGTTTAGCTACATCTTAAGAGACGCTGGCTTGGTGGATGCAGATGCAAGCGTTAACAACAAGCTAAGTCACAATCAGTCATTGGTCCGCGCAATCATATGTTCCGGACTATTCCCTGGCATAGCATCTGTAGTGCACCGAGAGACATCCATGTCTTTCAAAACTATGGATGATGGCCAGGTTTTACTATATGCTAATTCTGTAAATGCACGTTACCAAACAATTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAGTAAAGGTCAACACAGTTTTCATACGCGATTCTACTGGTGTCTCTGATTCCATTTTGATCCTTTTTGGTGGTGCCCTAAACCATGGTATCCAGGCTGGGCATTTGAAAATGTTGGACGGCTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGCTTAAAGAAGAGCTTAATGAGCTTATTCAGAAGAAGCTTCAAGATCCAAGCCTCGATATCCACAAGGAAGGAAAGTATCTCGTACTTGCTGTTCAAGAGCTGGTATCTGGAGATCAAAGTGAAGGAAGATTTGTGTTTGGCCGTGAAAACAGGAAGCCAAAGGAGTCCGGTGATAACAGCAGATTCACAAGGGATGGAACCAATCCTAAGAGTTTGCTACAAACTCTATTGATGAGAGCAGGACATTCACCTCCAAAATACAAAACCAAGCACCTCAAGACAAATGAGTTCAGAGCACTTGTGGAGTTCAAGGGCATGCAATTTGTTGGGAAGCCCAAGAAAAATAAGCAGCTTGCCGAAAGGGATGCTGCCATAGAAGCACTGGCCTGGTTGACTCACACCTCAGACAACAGTCGTGATGAAGATGACAACTCCCCACCTGATGTCACTAACAACATGCTGAAACTTCTGGGGAAACGTAGAAGATCAAAGCGCCACTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1148

Amino Acids

129.04

Weight (kDa)

8.81

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 258 - 412 2.3e-09 DEAD/DEAH box helicase
Helicase_C PF00271 519 - 648 3.6e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 712 - 740 2.8e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 741 - 801 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 868 - 949 5.4e-17 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 958 - 1016 3.2e-23 C-terminal domain in RNA helicases
dsrm PF00035 1049 - 1111 7.7e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2668
Acc16I TGCGCA 2 cut(s) 281, 677
Acc36I ACCTGC 1 cut(s) 2056
AccB1I GGYRCC 1 cut(s) 2843
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 1882
AccII CGCG 5 cut(s) 153, 1500, 1739, 2607, 2799
AccIII TCCGGA 2 cut(s) 738, 2621
AcoI YGGCCR 7 cut(s) 237, 883, 1375, 1583, 1936, 2692, 3079
AcsI RAATTY 7 cut(s) 77, 1125, 1246, 2035, 2084, 2179, 2470
AcuI CTGAAG 2 cut(s) 644, 735
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 6 cut(s) 93, 913, 1051, 1323, 1758, 3024
AfiI CCNNNNNNNGG 6 cut(s) 439, 1664, 2231, 2232, 2751, 2755
AflII CTTAAG 1 cut(s) 2533
AflIII ACRYGT 1 cut(s) 1108
AjiI CACGTC 1 cut(s) 1716
AjnI CCWGG 6 cut(s) 1585, 1948, 2632, 2694, 2862, 3326
Alw21I GWGCWC 5 cut(s) 582, 1264, 1498, 2655, 3238
Alw26I GTCTC 8 cut(s) 21, 324, 815, 1735, 1841, 2532, 2654, 2818
Alw44I GTGCAC 1 cut(s) 2651
AlwNI CAGNNNCTG 1 cut(s) 1592
Aor13HI TCCGGA 2 cut(s) 738, 2621
AoxI GGCC 8 cut(s) 237, 883, 1375, 1583, 1936, 2692, 3079, 3324
ApaLI GTGCAC 1 cut(s) 2651
ApeKI GCWGC 8 cut(s) 170, 274, 277, 778, 1945, 2096, 3289, 3308
ApoI RAATTY 7 cut(s) 77, 1125, 1246, 2035, 2084, 2179, 2470
AspLEI GCGC 7 cut(s) 153, 282, 453, 678, 1414, 2609, 3435
AspS9I GGNCC 2 cut(s) 292, 2602
AsuC2I CCSGG 3 cut(s) 1281, 1940, 2309
AsuHPI GGTGA 8 cut(s) 59, 581, 702, 995, 1679, 2777, 3122, 3180
AsuII TTCGAA 2 cut(s) 146, 210
AvaII GGWCC 2 cut(s) 292, 2602
AxyI CCTNAGG 1 cut(s) 2021
BaeGI GKGCMC 2 cut(s) 2655, 2848
BaeI ACNNNNGTAYC 2 cut(s) 2842, 2875
BalI TGGCCA 3 cut(s) 239, 1377, 2694
BamHI GGATCC 2 cut(s) 2311, 2911
BanI GGYRCC 1 cut(s) 2843
BbsI GAAGAC 1 cut(s) 1155
Bbv12I GWGCWC 5 cut(s) 582, 1264, 1498, 2655, 3238
BbvI GCAGC 8 cut(s) 157, 261, 264, 765, 1957, 2083, 3295, 3301
BccI CCATC 8 cut(s) 358, 629, 760, 1662, 2415, 2684, 2917, 3128
BceAI ACGGC 7 cut(s) 870, 1056, 1173, 1536, 1598, 2905, 3066
BcgI CGANNNNNNTGC 2 cut(s) 3287, 3321
BciT130I CCWGG 6 cut(s) 1587, 1950, 2634, 2696, 2864, 3328
BciVI GTATCC 1 cut(s) 2870
BclI TGATCA 1 cut(s) 2389
BcnI CCSGG 3 cut(s) 1281, 1940, 2309
BcoDI GTCTC 8 cut(s) 21, 324, 815, 1735, 1841, 2532, 2654, 2818
BfaI CTAG 7 cut(s) 86, 731, 863, 1445, 2231, 2364, 2916
BfmI CTRYAG 3 cut(s) 898, 960, 2646
BfoI RGCGCY 3 cut(s) 454, 1415, 3436
BfrI CTTAAG 1 cut(s) 2533
BfuAI ACCTGC 1 cut(s) 2056
BfuI GTATCC 1 cut(s) 2870
BglII AGATCT 1 cut(s) 1174
Bme1390I CCNGG 9 cut(s) 1281, 1587, 1940, 1950, 2309, 2634, 2696, 2864, 3328
Bme18I GGWCC 2 cut(s) 292, 2602
BmgBI CACGTC 1 cut(s) 1716
BmgT120I GGNCC 2 cut(s) 292, 2602
BmiI GGNNCC 5 cut(s) 414, 2313, 2845, 2913, 3139
BmrFI CCNGG 9 cut(s) 1281, 1587, 1940, 1950, 2309, 2634, 2696, 2864, 3328
BmrI ACTGGG 2 cut(s) 1344, 1620
BmuI ACTGGG 2 cut(s) 1344, 1620
BoxI GACNNNNGTC 1 cut(s) 3351
BpiI GAAGAC 1 cut(s) 1155
BplI GAGNNNNNCTC 6 cut(s) 167, 199, 1207, 1239, 1732, 1764
BpmI CTGGAG 2 cut(s) 2485, 3069
Bpu14I TTCGAA 2 cut(s) 146, 210
BpuEI CTTGAG 4 cut(s) 1114, 2132, 2313, 3200
BpuMI CCSGG 3 cut(s) 1281, 1940, 2309
BsaAI YACGTR 1 cut(s) 1760
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 2 cut(s) 1735, 1841
BsaJI CCNNGG 7 cut(s) 234, 886, 1415, 1699, 2632, 2750, 2854
BsaWI WCCGGW 3 cut(s) 738, 2621, 3107
Bsc4I CCNNNNNNNGG 6 cut(s) 439, 1664, 2231, 2232, 2751, 2755
Bse1I ACTGG 9 cut(s) 829, 1053, 1295, 1329, 1339, 1615, 2216, 2812, 3327
Bse21I CCTNAGG 1 cut(s) 2021
Bse3DI GCAATG 1 cut(s) 2263
BseAI TCCGGA 2 cut(s) 738, 2621
BseBI CCWGG 6 cut(s) 1587, 1950, 2634, 2696, 2864, 3328
BseDI CCNNGG 7 cut(s) 234, 886, 1415, 1699, 2632, 2750, 2854
BseLI CCNNNNNNNGG 6 cut(s) 439, 1664, 2231, 2232, 2751, 2755
BseMI GCAATG 1 cut(s) 2263
BseMII CTCAG 3 cut(s) 257, 857, 3357
BseNI ACTGG 9 cut(s) 829, 1053, 1295, 1329, 1339, 1615, 2216, 2812, 3327
BseRI GAGGAG 1 cut(s) 1272
BseSI GKGCMC 2 cut(s) 2655, 2848
BseXI GCAGC 8 cut(s) 157, 261, 264, 765, 1957, 2083, 3295, 3301
BseYI CCCAGC 1 cut(s) 2867
BsgI GTGCAG 1 cut(s) 1964
Bsh1236I CGCG 5 cut(s) 153, 1500, 1739, 2607, 2799
BshFI GGCC 8 cut(s) 239, 885, 1377, 1585, 1938, 2694, 3081, 3326
BshNI GGYRCC 1 cut(s) 2843
BsiHKAI GWGCWC 5 cut(s) 582, 1264, 1498, 2655, 3238
BsiSI CCGG 7 cut(s) 162, 739, 1280, 1939, 2308, 2622, 3108
BslFI GGGAC 2 cut(s) 381, 2365
BslI CCNNNNNNNGG 6 cut(s) 439, 1664, 2231, 2232, 2751, 2755
BsmAI GTCTC 8 cut(s) 21, 324, 815, 1735, 1841, 2532, 2654, 2818
BsmBI CGTCTC 2 cut(s) 21, 2532
BsmFI GGGAC 2 cut(s) 381, 2365
BsmI GAATGC 3 cut(s) 622, 893, 1234
BsnI GGCC 8 cut(s) 239, 885, 1377, 1585, 1938, 2694, 3081, 3326
Bso31I GGTCTC 2 cut(s) 1735, 1841
Bsp119I TTCGAA 2 cut(s) 146, 210
Bsp1286I GDGCHC 6 cut(s) 582, 1264, 1498, 2655, 2848, 3238
Bsp13I TCCGGA 2 cut(s) 738, 2621
Bsp1407I TGTACA 1 cut(s) 911
Bsp19I CCATGG 2 cut(s) 1699, 2854
Bsp68I TCGCGA 1 cut(s) 1739
BspANI GGCC 8 cut(s) 239, 885, 1377, 1585, 1938, 2694, 3081, 3326
BspCNI CTCAG 3 cut(s) 258, 856, 3356
BspEI TCCGGA 2 cut(s) 738, 2621
BspFNI CGCG 5 cut(s) 153, 1500, 1739, 2607, 2799
BspHI TCATGA 2 cut(s) 1129, 1605
BspLI GGNNCC 5 cut(s) 414, 2313, 2845, 2913, 3139
BspMAI CTGCAG 1 cut(s) 964
BspMI ACCTGC 1 cut(s) 2056
BspQI GCTCTTC 1 cut(s) 2943
BspT104I TTCGAA 2 cut(s) 146, 210
BspT107I GGYRCC 1 cut(s) 2843
BspTI CTTAAG 1 cut(s) 2533
BspTNI GGTCTC 2 cut(s) 1735, 1841
BsrBI CCGCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 2263
BsrGI TGTACA 1 cut(s) 911
BsrI ACTGG 9 cut(s) 829, 1053, 1295, 1329, 1339, 1615, 2216, 2812, 3327
BssECI CCNNGG 7 cut(s) 234, 886, 1415, 1699, 2632, 2750, 2854
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 3 cut(s) 1699, 2750, 2854
Bst2UI CCWGG 6 cut(s) 1587, 1950, 2634, 2696, 2864, 3328
Bst4CI ACNGT 5 cut(s) 575, 991, 1389, 2788, 3353
Bst6I CTCTTC 6 cut(s) 46, 124, 423, 633, 2514, 2943
BstACI GRCGYC 1 cut(s) 14
BstAFI CTTAAG 1 cut(s) 2533
BstAUI TGTACA 1 cut(s) 911
BstBAI YACGTR 1 cut(s) 1760
BstBI TTCGAA 2 cut(s) 146, 210
BstDSI CCRYGG 4 cut(s) 234, 886, 1699, 2854
BstENI CCTNNNNNAGG 1 cut(s) 1662
BstFNI CGCG 5 cut(s) 153, 1500, 1739, 2607, 2799
BstH2I RGCGCY 3 cut(s) 454, 1415, 3436
BstHHI GCGC 7 cut(s) 153, 282, 453, 678, 1414, 2609, 3435
BstMAI GTCTC 8 cut(s) 21, 324, 815, 1735, 1841, 2532, 2654, 2818
BstMWI GCNNNNNNNGC 5 cut(s) 688, 1411, 2354, 2959, 3317
BstNI CCWGG 6 cut(s) 1587, 1950, 2634, 2696, 2864, 3328
BstNSI RCATGY 5 cut(s) 682, 1112, 1698, 3259, 3400
BstPAI GACNNNNGTC 1 cut(s) 3351
BstSCI CCNGG 9 cut(s) 1279, 1585, 1938, 1948, 2307, 2632, 2694, 2862, 3326
BstSFI CTRYAG 3 cut(s) 898, 960, 2646
BstSLI GKGCMC 2 cut(s) 2655, 2848
BstSNI TACGTA 1 cut(s) 1760
BstUI CGCG 5 cut(s) 153, 1500, 1739, 2607, 2799
BstV1I GCAGC 8 cut(s) 157, 261, 264, 765, 1957, 2083, 3295, 3301
BstV2I GAAGAC 1 cut(s) 1155
BstX2I RGATCY 6 cut(s) 1174, 1195, 2221, 2311, 2911, 2983
BstXI CCANNNNNNTGG 1 cut(s) 364
BstYI RGATCY 6 cut(s) 1174, 1195, 2221, 2311, 2911, 2983
Bsu36I CCTNAGG 1 cut(s) 2021
BsuI GTATCC 1 cut(s) 2870
BsuRI GGCC 8 cut(s) 239, 885, 1377, 1585, 1938, 2694, 3081, 3326
BtgI CCRYGG 4 cut(s) 234, 886, 1699, 2854
BtrI CACGTC 1 cut(s) 1716
BtsI GCAGTG 2 cut(s) 289, 1437
BtsIMutI CAGTG 8 cut(s) 289, 375, 580, 996, 1060, 1302, 1437, 3320
BtuMI TCGCGA 1 cut(s) 1739
BveI ACCTGC 1 cut(s) 2056
CaiI CAGNNNCTG 1 cut(s) 1592
CciI TCATGA 2 cut(s) 1129, 1605
CfoI GCGC 7 cut(s) 153, 282, 453, 678, 1414, 2609, 3435
Cfr13I GGNCC 2 cut(s) 292, 2602
CseI GACGC 2 cut(s) 3, 2549
Csp6I GTAC 6 cut(s) 92, 912, 1050, 1322, 1757, 3023
CspCI CAANNNNNGTGG 2 cut(s) 547, 582
CviQI GTAC 6 cut(s) 92, 912, 1050, 1322, 1757, 3023
DrdI GACNNNNNNGTC 1 cut(s) 2668
DseDI GACNNNNNNGTC 1 cut(s) 2668
EaeI YGGCCR 7 cut(s) 237, 883, 1375, 1583, 1936, 2692, 3079
Eam1104I CTCTTC 6 cut(s) 46, 124, 423, 633, 2514, 2943
EarI CTCTTC 6 cut(s) 46, 124, 423, 633, 2514, 2943
Eco105I TACGTA 1 cut(s) 1760
Eco130I CCWWGG 3 cut(s) 1699, 2750, 2854
Eco31I GGTCTC 2 cut(s) 1735, 1841
Eco32I GATATC 4 cut(s) 813, 1624, 1899, 2998
Eco47I GGWCC 2 cut(s) 292, 2602
Eco57I CTGAAG 2 cut(s) 644, 735
Eco81I CCTNAGG 1 cut(s) 2021
EcoNI CCTNNNNNAGG 1 cut(s) 1662
EcoRI GAATTC 2 cut(s) 2035, 2084
EcoRII CCWGG 6 cut(s) 1585, 1948, 2632, 2694, 2862, 3326
EcoRV GATATC 4 cut(s) 813, 1624, 1899, 2998
EcoT14I CCWWGG 3 cut(s) 1699, 2750, 2854
ErhI CCWWGG 3 cut(s) 1699, 2750, 2854
Esp3I CGTCTC 2 cut(s) 21, 2532
FalI AAGNNNNNCTT 8 cut(s) 504, 536, 1164, 1196, 2014, 2046, 2937, 2969
FaqI GGGAC 2 cut(s) 381, 2365
FauI CCCGC 1 cut(s) 2341
FauNDI CATATG 5 cut(s) 1855, 2394, 2413, 2452, 2615
FbaI TGATCA 1 cut(s) 2389
FblI GTMKAC 1 cut(s) 1882
FspBI CTAG 7 cut(s) 86, 731, 863, 1445, 2231, 2364, 2916
FspI TGCGCA 2 cut(s) 281, 677
GlaI GCGC 7 cut(s) 152, 281, 452, 677, 1413, 2608, 3434
GsaI CCCAGC 1 cut(s) 2871
GsuI CTGGAG 2 cut(s) 2485, 3069
HaeII RGCGCY 3 cut(s) 454, 1415, 3436
HaeIII GGCC 8 cut(s) 239, 885, 1377, 1585, 1938, 2694, 3081, 3326
HapII CCGG 7 cut(s) 162, 739, 1280, 1939, 2308, 2622, 3108
HgaI GACGC 2 cut(s) 3, 2549
HhaI GCGC 7 cut(s) 153, 282, 453, 678, 1414, 2609, 3435
Hin1I GRCGYC 1 cut(s) 14
Hin6I GCGC 7 cut(s) 151, 280, 451, 676, 1412, 2607, 3433
HinP1I GCGC 7 cut(s) 151, 280, 451, 676, 1412, 2607, 3433
HincII GTYRAC 3 cut(s) 2572, 2782, 3333
HindII GTYRAC 3 cut(s) 2572, 2782, 3333
HindIII AAGCTT 2 cut(s) 2939, 2975
HpaI GTTAAC 1 cut(s) 2572
HpaII CCGG 7 cut(s) 162, 739, 1280, 1939, 2308, 2622, 3108
HphI GGTGA 8 cut(s) 59, 581, 702, 995, 1679, 2777, 3122, 3180
Hpy166II GTNNAC 7 cut(s) 506, 914, 1883, 2572, 2653, 2782, 3333
Hpy8I GTNNAC 7 cut(s) 506, 914, 1883, 2572, 2653, 2782, 3333
Hpy99I CGWCG 3 cut(s) 191, 194, 1194
HpyCH4III ACNGT 5 cut(s) 575, 991, 1389, 2788, 3353
HpyCH4IV ACGT 5 cut(s) 549, 1715, 1759, 2728, 3418
HpyF10VI GCNNNNNNNGC 5 cut(s) 688, 1411, 2354, 2959, 3317
HpySE526I ACGT 5 cut(s) 549, 1715, 1759, 2728, 3418
Hsp92I GRCGYC 1 cut(s) 14
HspAI GCGC 7 cut(s) 151, 280, 451, 676, 1412, 2607, 3433
Kpn2I TCCGGA 2 cut(s) 738, 2621
Ksp22I TGATCA 1 cut(s) 2389
KspAI GTTAAC 1 cut(s) 2572
LguI GCTCTTC 1 cut(s) 2943
LmnI GCTCC 4 cut(s) 175, 890, 1234, 1259
Lsp1109I GCAGC 8 cut(s) 157, 261, 264, 765, 1957, 2083, 3295, 3301
MaeI CTAG 7 cut(s) 86, 731, 863, 1445, 2231, 2364, 2916
MaeII ACGT 5 cut(s) 549, 1715, 1759, 2728, 3418
MaeIII GTNAC 7 cut(s) 284, 569, 1102, 1958, 2585, 2729, 3385
MbiI CCGCTC 1 cut(s) 8
MflI RGATCY 6 cut(s) 1174, 1195, 2221, 2311, 2911, 2983
MhlI GDGCHC 6 cut(s) 582, 1264, 1498, 2655, 2848, 3238
MlsI TGGCCA 3 cut(s) 239, 1377, 2694
MluNI TGGCCA 3 cut(s) 239, 1377, 2694
MlyI GAGTC 4 cut(s) 875, 2020, 3113, 3328
MmeI TCCRAC 6 cut(s) 69, 121, 210, 1496, 1553, 2865
Mox20I TGGCCA 3 cut(s) 239, 1377, 2694
MroI TCCGGA 2 cut(s) 738, 2621
MscI TGGCCA 3 cut(s) 239, 1377, 2694
MseI TTAA 7 cut(s) 1539, 1647, 2534, 2571, 2943, 2955, 3445
MslI CAYNNNNRTG 1 cut(s) 1510
Msp20I TGGCCA 3 cut(s) 239, 1377, 2694
MspA1I CMGCKG 2 cut(s) 1629, 2096
MspCI CTTAAG 1 cut(s) 2533
MspI CCGG 7 cut(s) 162, 739, 1280, 1939, 2308, 2622, 3108
MspR9I CCNGG 9 cut(s) 1281, 1587, 1940, 1950, 2309, 2634, 2696, 2864, 3328
Mva1269I GAATGC 3 cut(s) 622, 893, 1234
MvaI CCWGG 6 cut(s) 1587, 1950, 2634, 2696, 2864, 3328
MvnI CGCG 5 cut(s) 153, 1500, 1739, 2607, 2799
MwoI GCNNNNNNNGC 5 cut(s) 688, 1411, 2354, 2959, 3317
NciI CCSGG 3 cut(s) 1281, 1940, 2309
NcoI CCATGG 2 cut(s) 1699, 2854
NdeI CATATG 5 cut(s) 1855, 2394, 2413, 2452, 2615
NlaIV GGNNCC 5 cut(s) 414, 2313, 2845, 2913, 3139
NmeAIII GCCGAG 1 cut(s) 1894
NmuCI GTSAC 4 cut(s) 284, 569, 2585, 3385
NruI TCGCGA 1 cut(s) 1739
NsbI TGCGCA 2 cut(s) 281, 677
NspI RCATGY 5 cut(s) 682, 1112, 1698, 3259, 3400
NspV TTCGAA 2 cut(s) 146, 210
PaeI GCATGC 2 cut(s) 682, 3259
PagI TCATGA 2 cut(s) 1129, 1605
PciI ACATGT 1 cut(s) 1108
PciSI GCTCTTC 1 cut(s) 2943
PctI GAATGC 3 cut(s) 622, 893, 1234
PfeI GAWTC 9 cut(s) 623, 707, 766, 878, 1502, 2367, 2801, 2819, 3123
PflFI GACNNNGTC 1 cut(s) 1714
PfoI TCCNGGA 1 cut(s) 2307
PleI GAGTC 4 cut(s) 874, 2020, 3112, 3328
PpsI GAGTC 4 cut(s) 874, 2020, 3112, 3328
Ppu21I YACGTR 1 cut(s) 1760
PscI ACATGT 1 cut(s) 1108
PshAI GACNNNNGTC 1 cut(s) 3351
Psp6I CCWGG 6 cut(s) 1585, 1948, 2632, 2694, 2862, 3326
PspFI CCCAGC 1 cut(s) 2867
PspGI CCWGG 6 cut(s) 1585, 1948, 2632, 2694, 2862, 3326
PspN4I GGNNCC 5 cut(s) 414, 2313, 2845, 2913, 3139
PspPI GGNCC 2 cut(s) 292, 2602
PsrI GAACNNNNNNTAC 2 cut(s) 3015, 3047
PstI CTGCAG 1 cut(s) 964
PstNI CAGNNNCTG 1 cut(s) 1592
PsuI RGATCY 6 cut(s) 1174, 1195, 2221, 2311, 2911, 2983
PsyI GACNNNGTC 1 cut(s) 1714
PvuII CAGCTG 2 cut(s) 1629, 2096
RruI TCGCGA 1 cut(s) 1739
RsaI GTAC 6 cut(s) 93, 913, 1051, 1323, 1758, 3024
RsaNI GTAC 6 cut(s) 92, 912, 1050, 1322, 1757, 3023
RseI CAYNNNNRTG 1 cut(s) 1510
SapI GCTCTTC 1 cut(s) 2943
SaqAI TTAA 7 cut(s) 1539, 1647, 2534, 2571, 2943, 2955, 3445
Sau96I GGNCC 2 cut(s) 292, 2602
SchI GAGTC 4 cut(s) 875, 2020, 3113, 3328
ScrFI CCNGG 9 cut(s) 1281, 1587, 1940, 1950, 2309, 2634, 2696, 2864, 3328
SduI GDGCHC 6 cut(s) 582, 1264, 1498, 2655, 2848, 3238
SfcI CTRYAG 3 cut(s) 898, 960, 2646
SfuI TTCGAA 2 cut(s) 146, 210
SinI GGWCC 2 cut(s) 292, 2602
SmiMI CAYNNNNRTG 1 cut(s) 1510
SmlI CTYRAG 5 cut(s) 1093, 2147, 2328, 2533, 3215
SmoI CTYRAG 5 cut(s) 1093, 2147, 2328, 2533, 3215
SnaBI TACGTA 1 cut(s) 1760
SphI GCATGC 2 cut(s) 682, 3259
SspI AATATT 1 cut(s) 2201
SspMI CTAG 7 cut(s) 86, 731, 863, 1445, 2231, 2364, 2916
StyD4I CCNGG 9 cut(s) 1279, 1585, 1938, 1948, 2307, 2632, 2694, 2862, 3326
StyI CCWWGG 3 cut(s) 1699, 2750, 2854
TaaI ACNGT 5 cut(s) 575, 991, 1389, 2788, 3353
TaiI ACGT 5 cut(s) 552, 1718, 1762, 2731, 3421
TaqI TCGA 7 cut(s) 140, 146, 210, 922, 1006, 1469, 2994
TaqII GACCGA 1 cut(s) 575
TatI WGTACW 3 cut(s) 91, 911, 1321
TauI GCSGC 2 cut(s) 11, 14
TfiI GAWTC 9 cut(s) 623, 707, 766, 878, 1502, 2367, 2801, 2819, 3123
Tru1I TTAA 7 cut(s) 1539, 1647, 2534, 2571, 2943, 2955, 3445
Tru9I TTAA 7 cut(s) 1539, 1647, 2534, 2571, 2943, 2955, 3445
TscAI CASTG 8 cut(s) 289, 382, 580, 996, 1060, 1302, 1444, 3327
TseFI GTSAC 4 cut(s) 284, 569, 2585, 3385
TseI GCWGC 8 cut(s) 170, 274, 277, 778, 1945, 2096, 3289, 3308
Tsp45I GTSAC 4 cut(s) 284, 569, 2585, 3385
TspGWI ACGGA 2 cut(s) 223, 376
TspRI CASTG 8 cut(s) 289, 382, 580, 996, 1060, 1302, 1444, 3327
Tth111I GACNNNGTC 1 cut(s) 1714
Vha464I CTTAAG 1 cut(s) 2533
VneI GTGCAC 1 cut(s) 2651
VpaK11BI GGWCC 2 cut(s) 292, 2602
XagI CCTNNNNNAGG 1 cut(s) 1662
XapI RAATTY 7 cut(s) 77, 1125, 1246, 2035, 2084, 2179, 2470
XbaI TCTAGA 1 cut(s) 2363
XceI RCATGY 5 cut(s) 682, 1112, 1698, 3259, 3400
XcmI CCANNNNNNNNNTGG 1 cut(s) 3319
XmiI GTMKAC 1 cut(s) 1882
XspI CTAG 7 cut(s) 86, 731, 863, 1445, 2231, 2364, 2916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.