RLG00000034681

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005499
Physical Location & Seq
Forward (+)
48293654 .. 48326014
32361 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034681

Sequence Viewer

Length: 3081 bp
ATGCACAAATCGAGAGCGCCAGCATCTCTCCTTGTCAAATCAGTAGCCTCCTCCAACAATACCTGTTTTCGTTCTCACCACCTCACATTCTGTCTTTTACTCCAACCACAACCAGGCTCTTCTTCTTCTTCCTCTTCTCCGACTTTGAACGGCAGATTCCTCATGTCGAAGCGCGGCTTCTCCGGCTACGCTGCGGAGCAGTTCTCCGACGACGAGTATGAGTGCGATTTCGAAAGCCAAAAGGCTTCATCTTCCGTGGCCAACATTGACGAGTGGAAATGGAAACTAAGCCTGCTGCTGCGCGGTGACAAGGACCAAGAGATTGTTTCCAGAGATAAAAGGGACAGGAGAGACTATGACAAGGTTCCTCTTCCAAACTACCGCCCCGATTTGGATGATAAGCGCCCACAAAGAGAGGTATTTATTTATTTATTTATTGTCTTCTTCCAACACCTTGATCGTGTCCTGCTAAGTTCCCGCAACGTATCAGCCAATTCAGGTGACAGTGAGCACCTCGATCAGCTTGAAAATGAAAAGCCTGATGAGAATGCAGATTCTTTTCTTGATGGGTCTGTAATGGAGAAGGTTCTTCAGCGTAGGAGTTTGCGAATGCGTAATATGCAAAGAGCTTGGCAGGAATCACCTGAAGGTAAAAAGATGCTAGAGTTCCGGAAATCACTACCATCTTTCAAAGAGAATCAGAGGTTGCTGCAAGCAATCGCACTAAATCAGGTAGTAGTGATATCTGGAGAGACTGGATGTGGTAAGACCACTCAGCGTCCTCAATATATACTAGAGTCAGAGATAGAATCTGGCCGTGGAGCATTCTGTAGCATTCTCTGTACACAACCTCGAAGAATATCTGCTATGGCTGTTGCTGAAAGAGTGTCTGCAGAGAGGGGAGAGCCTCTTGGTGAAACAGTGGGCTATAAAGTTCGATTAGAGGGAATGAAAGGAAAAAATACGCATCTCCTTTTCTGTACCAGTGGTATTCTACTTCGCCGTCTGTTGAGTGATAGGAACTTGAGTGGTGGTTTCACATATCCAGTGAAAGCACACTTTTTGGAAGATGTACTGGAAATGACTGGGTATAAGCTGACTTCTTTCAACCAAATTGATGACTATGGCCAGGACAAACTGTGGAAAACGCAGAAGCAGTTGGCTCCTCGGAAAAGGAAAAACCAGATCACTGCTCTAGTTGAGGATGCTCTTAGTAAATCAAGCTTTGAGAATTATAGCCCCAGAGCACGTGATTCATTATCATGTTGGACAGCTGATTGCATAGGATTTAATCTTATTGAAGCTGTTTTATGTCATATATGTCGGAAGGAACGGCCAGGTGCTGTCTTAGTATTCATGACTGGGTGGGCGGATATCAGCTGTTTGAGGGATCAACTTAAAACCCATCCTCTTTTAGGTGATCCTAATAGGGTTTTGCTTATAACATGCCATGGGTCAATGGCCACGTCTGAACAGAACCTCATTTTTGGGAGACCACCTCCTAATGTACGTAAAATAGTTCTTGCTACTAATATGGCAGAAGCAAGCATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCACATATGATGCTTTGAATAATACACCTTGTCTACTACCTTCTTGGATATCCCAAGCATCTGCTCGGCAAAGAAGGGGAAGGGCTGGCCGAGTGCAGCCAGGCGAAAGTTACCACCTTTACCCCAAATGTGTGTATCATGCTTTTGCGGAATACCAACTTCCTGAACTTTTAAGGACTCCTTTGAATTCTCTCTGCTTGCAAATCAAAAGTATGCAGATTGGTAGTATAGGAGAATTCTTGTCAGCTGCCCTGCAACCACCTGAACCCCTTGCTGTTCAAAATGCTGTTGGCTTTCTTAAGTTGATTGGGGCATTGGATGAGAATGAAAATTTGACAAATCTCGGAAAATATTTGTCAATGCTTCCAGTAGATCCCAAACTAGGGAAAATGCTGATTATGGGTGCTGTATTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGGCTAAGTGTCCGGGATCCATTCCTTTTGCCTCAGGACAAAAAAGATTTAGCGGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTACAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCAAAAAGAGAAGGATCAACGTATGAATACTGCTGGAGAAATTTTCTCTCTGCCCAAACACTTCAAGCTATCCATTCTCTTCGGAAGCAGTTTAGCTACATCTTAAGAGACGCTGGCTTGGTGGATGCGGATGCAACCATTAACAACAAGCTAAGTCACAATCAGTCATTGGTCCGCGCAATCATATGTTCTGGACTCTTCCCTGGCATAGCATCTGTAGTGCACCGAGAGACATCCATGTCTTTCAAAACTATGGATGATGGCCAGGTTTTACTATATGCTAATTCTGTAAATGCACGTTACCAAACAGTTCCCTACCCTTGGCTGGTCTTTGGTGAAAAAGTAAAGGTCAACACTGTTTTCATACGCGATTCTACTGGTGTCTCTGATTCCATTTTGATCCTTTTTGGTGGTGCCCTAATCCATGGGATCCAGGCTGGGCATTTGAAAATGTTGGACGGCTATATTGACTTCTTCATGGATCCTAGTTTGATGGACTGCTATTTGAAGCTTAAAGAAGAGCTTAATGAGCTTATTCAGAAGAAGCTTCAAGATCCAAGCCTCAATATCCACAAGGAAGGAAAGTATCTCATACTTGCTGTTCAAGAGCTGCTATCTGGAGATCAAAGTGAAGGAAGATTTGTGTTTGGCCGTGAAAACAGGAAGCCAAAGGAGTCTGGTGATAACAGCAGATTCACAAGGGATGGAACCAATCCTAAGAGTTTGCTACAAACTTTATTGATGAGAGCAAGACATTCACCTCCAAAATACAAAACCAAGCACCTCAAGACAAATGAGTTCAGAGCACTAGTGGAGTTCAAGGGCATGCAATTTGTTGGGAAGCCCAAGAAAAATAAGCAGCTTGCCGAAAGGGATGCTGCCATAGAAGCACTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1027

Amino Acids

115.5

Weight (kDa)

8.99

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 436 - 565 8e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 629 - 657 2.5e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 658 - 718 5.1e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 785 - 866 1.5e-16 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 875 - 933 7.7e-23 C-terminal domain in RNA helicases
dsrm PF00035 966 - 1025 3.2e-06 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1442
AasI GACNNNNNNGTC 1 cut(s) 2419
AccB1I GGYRCC 1 cut(s) 2594
AccI GTMKAC 1 cut(s) 1633
AccII CGCG 4 cut(s) 174, 303, 2358, 2550
AccIII TCCGGA 1 cut(s) 669
AcoI YGGCCR 8 cut(s) 258, 814, 1126, 1334, 1461, 1687, 2443, 2830
AcsI RAATTY 4 cut(s) 1786, 1835, 1930, 2221
AcuI CTGAAG 2 cut(s) 575, 666
AcvI CACGTG 1 cut(s) 1250
AfaI GTAC 4 cut(s) 844, 982, 1074, 1509
AfiI CCNNNNNNNGG 7 cut(s) 113, 391, 1415, 1982, 1983, 2502, 2506
AflII CTTAAG 2 cut(s) 1898, 2284
AhlI ACTAGT 1 cut(s) 2989
AjiI CACGTC 1 cut(s) 1467
AjnI CCWGG 7 cut(s) 112, 1128, 1336, 1699, 2383, 2445, 2613
Alw21I GWGCWC 4 cut(s) 513, 1249, 2406, 2989
Alw26I GTCTC 7 cut(s) 345, 746, 1486, 1592, 2283, 2405, 2569
Alw44I GTGCAC 1 cut(s) 2402
AlwNI CAGNNNCTG 1 cut(s) 1343
Aor13HI TCCGGA 1 cut(s) 669
AoxI GGCC 9 cut(s) 258, 814, 1126, 1334, 1461, 1687, 2443, 2830, 3075
ApaLI GTGCAC 1 cut(s) 2402
ApeKI GCWGC 9 cut(s) 191, 295, 298, 709, 1696, 1847, 2791, 3040, 3059
ApoI RAATTY 4 cut(s) 1786, 1835, 1930, 2221
AspLEI GCGC 5 cut(s) 19, 174, 303, 405, 2360
AspS9I GGNCC 2 cut(s) 313, 2353
AsuC2I CCSGG 1 cut(s) 2060
AsuHPI GGTGA 9 cut(s) 68, 317, 512, 633, 926, 1430, 2528, 2873, 2931
AsuII TTCGAA 1 cut(s) 231
AvaII GGWCC 2 cut(s) 313, 2353
AxyI CCTNAGG 1 cut(s) 2079
BaeGI GKGCMC 2 cut(s) 2406, 2599
BalI TGGCCA 4 cut(s) 260, 1128, 1463, 2445
BamHI GGATCC 3 cut(s) 2062, 2610, 2662
BanI GGYRCC 1 cut(s) 2594
BbrPI CACGTG 1 cut(s) 1250
BbsI GAAGAC 1 cut(s) 433
Bbv12I GWGCWC 4 cut(s) 513, 1249, 2406, 2989
BbvI GCAGC 9 cut(s) 178, 282, 285, 696, 1708, 1834, 2778, 3046, 3052
BccI CCATC 7 cut(s) 560, 691, 1413, 2166, 2435, 2668, 2879
BceAI ACGGC 6 cut(s) 166, 801, 987, 1349, 2656, 2817
BcgI CGANNNNNNTGC 2 cut(s) 3038, 3072
BciT130I CCWGG 7 cut(s) 114, 1130, 1338, 1701, 2385, 2447, 2615
BclI TGATCA 1 cut(s) 2140
BcnI CCSGG 1 cut(s) 2060
BcoDI GTCTC 7 cut(s) 345, 746, 1486, 1592, 2283, 2405, 2569
BcuI ACTAGT 1 cut(s) 2989
BfaI CTAG 8 cut(s) 662, 794, 1196, 1982, 2115, 2667, 2990, 3079
BfmI CTRYAG 3 cut(s) 829, 891, 2397
BfoI RGCGCY 2 cut(s) 20, 406
BfrI CTTAAG 2 cut(s) 1898, 2284
Bme1390I CCNGG 8 cut(s) 114, 1130, 1338, 1701, 2060, 2385, 2447, 2615
Bme18I GGWCC 2 cut(s) 313, 2353
BmgBI CACGTC 1 cut(s) 1467
BmgT120I GGNCC 2 cut(s) 313, 2353
BmiI GGNNCC 7 cut(s) 366, 1164, 2064, 2596, 2612, 2664, 2890
BmrFI CCNGG 8 cut(s) 114, 1130, 1338, 1701, 2060, 2385, 2447, 2615
BmrI ACTGGG 2 cut(s) 1095, 1371
BmuI ACTGGG 2 cut(s) 1095, 1371
BpiI GAAGAC 1 cut(s) 433
BplI GAGNNNNNCTC 4 cut(s) 188, 220, 1483, 1515
BpmI CTGGAG 3 cut(s) 768, 2236, 2820
Bpu14I TTCGAA 1 cut(s) 231
BpuEI CTTGAG 2 cut(s) 1045, 2951
BpuMI CCSGG 1 cut(s) 2060
BsaAI YACGTR 2 cut(s) 1250, 1511
BsaI GGTCTC 2 cut(s) 1486, 1592
BsaJI CCNNGG 7 cut(s) 255, 817, 1166, 1450, 2383, 2501, 2605
BsaWI WCCGGW 1 cut(s) 669
Bsc4I CCNNNNNNNGG 7 cut(s) 113, 391, 1415, 1982, 1983, 2502, 2506
Bse1I ACTGG 9 cut(s) 760, 984, 1046, 1080, 1090, 1366, 1967, 2563, 3078
Bse21I CCTNAGG 1 cut(s) 2079
Bse3DI GCAATG 1 cut(s) 2014
BseAI TCCGGA 1 cut(s) 669
BseBI CCWGG 7 cut(s) 114, 1130, 1338, 1701, 2385, 2447, 2615
BseDI CCNNGG 7 cut(s) 255, 817, 1166, 1450, 2383, 2501, 2605
BseLI CCNNNNNNNGG 7 cut(s) 113, 391, 1415, 1982, 1983, 2502, 2506
BseMI GCAATG 1 cut(s) 2014
BseMII CTCAG 2 cut(s) 788, 2093
BseNI ACTGG 9 cut(s) 760, 984, 1046, 1080, 1090, 1366, 1967, 2563, 3078
BseRI GAGGAG 2 cut(s) 40, 1155
BseSI GKGCMC 2 cut(s) 2406, 2599
BseXI GCAGC 9 cut(s) 178, 282, 285, 696, 1708, 1834, 2778, 3046, 3052
BseYI CCCAGC 1 cut(s) 2618
BsgI GTGCAG 1 cut(s) 1715
Bsh1236I CGCG 4 cut(s) 174, 303, 2358, 2550
BshFI GGCC 9 cut(s) 260, 816, 1128, 1336, 1463, 1689, 2445, 2832, 3077
BshNI GGYRCC 1 cut(s) 2594
BsiHKAI GWGCWC 4 cut(s) 513, 1249, 2406, 2989
BsiSI CCGG 3 cut(s) 183, 670, 2059
BslFI GGGAC 2 cut(s) 356, 2116
BslI CCNNNNNNNGG 7 cut(s) 113, 391, 1415, 1982, 1983, 2502, 2506
BsmAI GTCTC 7 cut(s) 345, 746, 1486, 1592, 2283, 2405, 2569
BsmBI CGTCTC 1 cut(s) 2283
BsmFI GGGAC 2 cut(s) 356, 2116
BsmI GAATGC 4 cut(s) 553, 615, 824, 834
BsnI GGCC 9 cut(s) 260, 816, 1128, 1336, 1463, 1689, 2445, 2832, 3077
Bso31I GGTCTC 2 cut(s) 1486, 1592
Bsp119I TTCGAA 1 cut(s) 231
Bsp1286I GDGCHC 5 cut(s) 513, 1249, 2406, 2599, 2989
Bsp13I TCCGGA 1 cut(s) 669
Bsp1407I TGTACA 1 cut(s) 842
Bsp19I CCATGG 2 cut(s) 1450, 2605
BspANI GGCC 9 cut(s) 260, 816, 1128, 1336, 1463, 1689, 2445, 2832, 3077
BspCNI CTCAG 2 cut(s) 787, 2092
BspEI TCCGGA 1 cut(s) 669
BspFNI CGCG 4 cut(s) 174, 303, 2358, 2550
BspHI TCATGA 1 cut(s) 1356
BspLI GGNNCC 7 cut(s) 366, 1164, 2064, 2596, 2612, 2664, 2890
BspMAI CTGCAG 1 cut(s) 895
BspQI GCTCTTC 2 cut(s) 124, 2694
BspT104I TTCGAA 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 2594
BspTI CTTAAG 2 cut(s) 1898, 2284
BspTNI GGTCTC 2 cut(s) 1486, 1592
BsrDI GCAATG 1 cut(s) 2014
BsrGI TGTACA 1 cut(s) 842
BsrI ACTGG 9 cut(s) 760, 984, 1046, 1080, 1090, 1366, 1967, 2563, 3078
BssECI CCNNGG 7 cut(s) 255, 817, 1166, 1450, 2383, 2501, 2605
BssT1I CCWWGG 3 cut(s) 1450, 2501, 2605
Bst2UI CCWGG 7 cut(s) 114, 1130, 1338, 1701, 2385, 2447, 2615
Bst4CI ACNGT 6 cut(s) 506, 922, 1140, 2138, 2491, 2539
Bst6I CTCTTC 6 cut(s) 124, 139, 375, 2265, 2384, 2694
BstAFI CTTAAG 2 cut(s) 1898, 2284
BstAUI TGTACA 1 cut(s) 842
BstBAI YACGTR 2 cut(s) 1250, 1511
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 9 cut(s) 21, 293, 714, 1546, 1687, 1799, 2296, 3008, 3045
BstDEI CTNAG 9 cut(s) 287, 470, 774, 1211, 1348, 2051, 2079, 2333, 2898
BstDSI CCRYGG 4 cut(s) 255, 817, 1450, 2605
BstENI CCTNNNNNAGG 1 cut(s) 1413
BstFNI CGCG 4 cut(s) 174, 303, 2358, 2550
BstH2I RGCGCY 2 cut(s) 20, 406
BstHHI GCGC 5 cut(s) 19, 174, 303, 405, 2360
BstMAI GTCTC 7 cut(s) 345, 746, 1486, 1592, 2283, 2405, 2569
BstMWI GCNNNNNNNGC 5 cut(s) 183, 619, 2105, 2710, 3068
BstNI CCWGG 7 cut(s) 114, 1130, 1338, 1701, 2385, 2447, 2615
BstNSI RCATGY 2 cut(s) 1449, 3010
BstSCI CCNGG 8 cut(s) 112, 1128, 1336, 1699, 2058, 2383, 2445, 2613
BstSFI CTRYAG 3 cut(s) 829, 891, 2397
BstSLI GKGCMC 2 cut(s) 2406, 2599
BstSNI TACGTA 1 cut(s) 1511
BstUI CGCG 4 cut(s) 174, 303, 2358, 2550
BstV1I GCAGC 9 cut(s) 178, 282, 285, 696, 1708, 1834, 2778, 3046, 3052
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 5 cut(s) 1972, 2062, 2610, 2662, 2734
BstYI RGATCY 5 cut(s) 1972, 2062, 2610, 2662, 2734
Bsu36I CCTNAGG 1 cut(s) 2079
BsuRI GGCC 9 cut(s) 260, 816, 1128, 1336, 1463, 1689, 2445, 2832, 3077
BtgI CCRYGG 4 cut(s) 255, 817, 1450, 2605
BtrI CACGTC 1 cut(s) 1467
BtsI GCAGTG 1 cut(s) 1188
BtsIMutI CAGTG 8 cut(s) 511, 927, 991, 1053, 1188, 2143, 2535, 3071
Cac8I GCNNGC 9 cut(s) 21, 293, 714, 1546, 1687, 1799, 2296, 3008, 3045
CaiI CAGNNNCTG 1 cut(s) 1343
CciI TCATGA 1 cut(s) 1356
CfoI GCGC 5 cut(s) 19, 174, 303, 405, 2360
Cfr13I GGNCC 2 cut(s) 313, 2353
CseI GACGC 2 cut(s) 767, 2300
Csp6I GTAC 4 cut(s) 843, 981, 1073, 1508
CviQI GTAC 4 cut(s) 843, 981, 1073, 1508
DdeI CTNAG 9 cut(s) 287, 470, 774, 1211, 1348, 2051, 2079, 2333, 2898
DrdI GACNNNNNNGTC 1 cut(s) 2419
DseDI GACNNNNNNGTC 1 cut(s) 2419
EaeI YGGCCR 8 cut(s) 258, 814, 1126, 1334, 1461, 1687, 2443, 2830
Eam1104I CTCTTC 6 cut(s) 124, 139, 375, 2265, 2384, 2694
EarI CTCTTC 6 cut(s) 124, 139, 375, 2265, 2384, 2694
EciI GGCGGA 1 cut(s) 1385
Eco105I TACGTA 1 cut(s) 1511
Eco130I CCWWGG 3 cut(s) 1450, 2501, 2605
Eco31I GGTCTC 2 cut(s) 1486, 1592
Eco32I GATATC 3 cut(s) 744, 1375, 1650
Eco47I GGWCC 2 cut(s) 313, 2353
Eco57I CTGAAG 2 cut(s) 575, 666
Eco72I CACGTG 1 cut(s) 1250
Eco81I CCTNAGG 1 cut(s) 2079
EcoNI CCTNNNNNAGG 1 cut(s) 1413
EcoRI GAATTC 2 cut(s) 1786, 1835
EcoRII CCWGG 7 cut(s) 112, 1128, 1336, 1699, 2383, 2445, 2613
EcoRV GATATC 3 cut(s) 744, 1375, 1650
EcoT14I CCWWGG 3 cut(s) 1450, 2501, 2605
ErhI CCWWGG 3 cut(s) 1450, 2501, 2605
Esp3I CGTCTC 1 cut(s) 2283
FalI AAGNNNNNCTT 6 cut(s) 161, 193, 1765, 1797, 2688, 2720
FaqI GGGAC 2 cut(s) 356, 2116
FauI CCCGC 2 cut(s) 485, 2092
FauNDI CATATG 4 cut(s) 1606, 2145, 2164, 2366
FbaI TGATCA 1 cut(s) 2140
FblI GTMKAC 1 cut(s) 1633
FspBI CTAG 8 cut(s) 662, 794, 1196, 1982, 2115, 2667, 2990, 3079
GlaI GCGC 5 cut(s) 18, 173, 302, 404, 2359
GsaI CCCAGC 1 cut(s) 2622
GsuI CTGGAG 3 cut(s) 768, 2236, 2820
HaeII RGCGCY 2 cut(s) 20, 406
HaeIII GGCC 9 cut(s) 260, 816, 1128, 1336, 1463, 1689, 2445, 2832, 3077
HapII CCGG 3 cut(s) 183, 670, 2059
HgaI GACGC 2 cut(s) 767, 2300
HhaI GCGC 5 cut(s) 19, 174, 303, 405, 2360
Hin6I GCGC 5 cut(s) 17, 172, 301, 403, 2358
HinP1I GCGC 5 cut(s) 17, 172, 301, 403, 2358
HincII GTYRAC 1 cut(s) 2533
HindII GTYRAC 1 cut(s) 2533
HindIII AAGCTT 3 cut(s) 1222, 2690, 2726
HpaII CCGG 3 cut(s) 183, 670, 2059
HphI GGTGA 9 cut(s) 68, 317, 512, 633, 926, 1430, 2528, 2873, 2931
Hpy166II GTNNAC 4 cut(s) 845, 1634, 2404, 2533
Hpy8I GTNNAC 4 cut(s) 845, 1634, 2404, 2533
Hpy99I CGWCG 2 cut(s) 212, 215
HpyCH4III ACNGT 6 cut(s) 506, 922, 1140, 2138, 2491, 2539
HpyCH4IV ACGT 6 cut(s) 483, 1249, 1466, 1510, 2201, 2479
HpyF10VI GCNNNNNNNGC 5 cut(s) 183, 619, 2105, 2710, 3068
HpyF3I CTNAG 9 cut(s) 287, 470, 774, 1211, 1348, 2051, 2079, 2333, 2898
HpySE526I ACGT 6 cut(s) 483, 1249, 1466, 1510, 2201, 2479
HspAI GCGC 5 cut(s) 17, 172, 301, 403, 2358
Kpn2I TCCGGA 1 cut(s) 669
Ksp22I TGATCA 1 cut(s) 2140
LguI GCTCTTC 2 cut(s) 124, 2694
LmnI GCTCC 3 cut(s) 196, 821, 1168
Lsp1109I GCAGC 9 cut(s) 178, 282, 285, 696, 1708, 1834, 2778, 3046, 3052
MaeI CTAG 8 cut(s) 662, 794, 1196, 1982, 2115, 2667, 2990, 3079
MaeII ACGT 6 cut(s) 483, 1249, 1466, 1510, 2201, 2479
MaeIII GTNAC 5 cut(s) 305, 500, 1709, 2336, 2480
MflI RGATCY 5 cut(s) 1972, 2062, 2610, 2662, 2734
MhlI GDGCHC 5 cut(s) 513, 1249, 2406, 2599, 2989
MlsI TGGCCA 4 cut(s) 260, 1128, 1463, 2445
MluCI AATT 9 cut(s) 493, 1113, 1231, 1786, 1835, 1930, 2221, 2464, 3011
MluNI TGGCCA 4 cut(s) 260, 1128, 1463, 2445
MlyI GAGTC 4 cut(s) 806, 1771, 2370, 2864
MmeI TCCRAC 8 cut(s) 78, 127, 164, 231, 472, 1247, 1304, 2616
Mox20I TGGCCA 4 cut(s) 260, 1128, 1463, 2445
MroI TCCGGA 1 cut(s) 669
MscI TGGCCA 4 cut(s) 260, 1128, 1463, 2445
MseI TTAA 8 cut(s) 1290, 1398, 1772, 1899, 2285, 2322, 2694, 2706
MslI CAYNNNNRTG 1 cut(s) 1261
Msp20I TGGCCA 4 cut(s) 260, 1128, 1463, 2445
MspA1I CMGCKG 3 cut(s) 1274, 1380, 1847
MspCI CTTAAG 2 cut(s) 1898, 2284
MspI CCGG 3 cut(s) 183, 670, 2059
MspR9I CCNGG 8 cut(s) 114, 1130, 1338, 1701, 2060, 2385, 2447, 2615
Mva1269I GAATGC 4 cut(s) 553, 615, 824, 834
MvaI CCWGG 7 cut(s) 114, 1130, 1338, 1701, 2385, 2447, 2615
MvnI CGCG 4 cut(s) 174, 303, 2358, 2550
MwoI GCNNNNNNNGC 5 cut(s) 183, 619, 2105, 2710, 3068
NciI CCSGG 1 cut(s) 2060
NcoI CCATGG 2 cut(s) 1450, 2605
NdeI CATATG 4 cut(s) 1606, 2145, 2164, 2366
NlaIV GGNNCC 7 cut(s) 366, 1164, 2064, 2596, 2612, 2664, 2890
NmeAIII GCCGAG 2 cut(s) 1645, 1715
NmuCI GTSAC 3 cut(s) 305, 500, 2336
NspI RCATGY 2 cut(s) 1449, 3010
NspV TTCGAA 1 cut(s) 231
PaeI GCATGC 1 cut(s) 3010
PagI TCATGA 1 cut(s) 1356
PciSI GCTCTTC 2 cut(s) 124, 2694
PctI GAATGC 4 cut(s) 553, 615, 824, 834
PfoI TCCNGGA 1 cut(s) 2058
PleI GAGTC 4 cut(s) 805, 1771, 2370, 2863
PmaCI CACGTG 1 cut(s) 1250
PmlI CACGTG 1 cut(s) 1250
PpsI GAGTC 4 cut(s) 805, 1771, 2370, 2863
Ppu21I YACGTR 2 cut(s) 1250, 1511
PsiI TTATAA 1 cut(s) 1442
Psp6I CCWGG 7 cut(s) 112, 1128, 1336, 1699, 2383, 2445, 2613
PspCI CACGTG 1 cut(s) 1250
PspFI CCCAGC 1 cut(s) 2618
PspGI CCWGG 7 cut(s) 112, 1128, 1336, 1699, 2383, 2445, 2613
PspN4I GGNNCC 7 cut(s) 366, 1164, 2064, 2596, 2612, 2664, 2890
PspPI GGNCC 2 cut(s) 313, 2353
PstI CTGCAG 1 cut(s) 895
PstNI CAGNNNCTG 1 cut(s) 1343
PsuI RGATCY 5 cut(s) 1972, 2062, 2610, 2662, 2734
PvuII CAGCTG 3 cut(s) 1274, 1380, 1847
RsaI GTAC 4 cut(s) 844, 982, 1074, 1509
RsaNI GTAC 4 cut(s) 843, 981, 1073, 1508
RseI CAYNNNNRTG 1 cut(s) 1261
SapI GCTCTTC 2 cut(s) 124, 2694
SaqAI TTAA 8 cut(s) 1290, 1398, 1772, 1899, 2285, 2322, 2694, 2706
Sau96I GGNCC 2 cut(s) 313, 2353
SchI GAGTC 4 cut(s) 806, 1771, 2370, 2864
ScrFI CCNGG 8 cut(s) 114, 1130, 1338, 1701, 2060, 2385, 2447, 2615
SduI GDGCHC 5 cut(s) 513, 1249, 2406, 2599, 2989
SfcI CTRYAG 3 cut(s) 829, 891, 2397
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 2 cut(s) 313, 2353
SmiMI CAYNNNNRTG 1 cut(s) 1261
SmlI CTYRAG 4 cut(s) 1024, 1898, 2284, 2966
SmoI CTYRAG 4 cut(s) 1024, 1898, 2284, 2966
SnaBI TACGTA 1 cut(s) 1511
SpeI ACTAGT 1 cut(s) 2989
SphI GCATGC 1 cut(s) 3010
Sse9I AATT 9 cut(s) 493, 1113, 1231, 1786, 1835, 1930, 2221, 2464, 3011
SspI AATATT 1 cut(s) 1952
SspMI CTAG 8 cut(s) 662, 794, 1196, 1982, 2115, 2667, 2990, 3079
StyD4I CCNGG 8 cut(s) 112, 1128, 1336, 1699, 2058, 2383, 2445, 2613
StyI CCWWGG 3 cut(s) 1450, 2501, 2605
TaaI ACNGT 6 cut(s) 506, 922, 1140, 2138, 2491, 2539
TaiI ACGT 6 cut(s) 486, 1252, 1469, 1513, 2204, 2482
TaqI TCGA 6 cut(s) 11, 167, 231, 516, 853, 937
TasI AATT 9 cut(s) 493, 1113, 1231, 1786, 1835, 1930, 2221, 2464, 3011
TatI WGTACW 2 cut(s) 842, 1072
TauI GCSGC 1 cut(s) 177
Tru1I TTAA 8 cut(s) 1290, 1398, 1772, 1899, 2285, 2322, 2694, 2706
Tru9I TTAA 8 cut(s) 1290, 1398, 1772, 1899, 2285, 2322, 2694, 2706
TscAI CASTG 8 cut(s) 511, 927, 991, 1053, 1195, 2143, 2542, 3078
TseFI GTSAC 3 cut(s) 305, 500, 2336
TseI GCWGC 9 cut(s) 191, 295, 298, 709, 1696, 1847, 2791, 3040, 3059
Tsp45I GTSAC 3 cut(s) 305, 500, 2336
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 8 cut(s) 511, 927, 991, 1053, 1195, 2143, 2542, 3078
Vha464I CTTAAG 2 cut(s) 1898, 2284
VneI GTGCAC 1 cut(s) 2402
VpaK11BI GGWCC 2 cut(s) 313, 2353
XagI CCTNNNNNAGG 1 cut(s) 1413
XapI RAATTY 4 cut(s) 1786, 1835, 1930, 2221
XbaI TCTAGA 1 cut(s) 2114
XceI RCATGY 2 cut(s) 1449, 3010
XcmI CCANNNNNNNNNTGG 1 cut(s) 3070
XmiI GTMKAC 1 cut(s) 1633
XspI CTAG 8 cut(s) 662, 794, 1196, 1982, 2115, 2667, 2990, 3079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.