AT5G05500

pollen Ole e 1 allergen and extensin family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004472
Physical Location & Seq
Forward (+)
1629610 .. 1630478
869 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05500.1

Sequence Viewer

Length: 552 bp
ATGGACTCAAGAGCCTTACCCTTCTCCGTTCTCGTCATCCTCATTGCTACGGCGGTGTTGGCCAACGCTTACACTCCTCCTCCTCCTCCTCCAACCACCGCATACCCTGCCGTGAAAACCGTGGAAGCGGCAGTGGAAGGAATGGTGTACTGTCAATCCTGCGACAAATTCGGATCATGGTCATTGGCCGGAGCAGAAGCTATAGCCGGAGCTAAAATCAGCATTATCTGCAAGAATCATAGGCAACAAGTGAGTTTCTACAAAGTCTTTCGAACTGATTCCTACGGCCATTTCTATGGTGAGCTCAAAGGTTTCAAGATGACTCCACATTTCTTGGACCATCCTCTTCATTCTTGCCGAGCCAAGCTCGTCTCTTCTCCTCGTGAAGACTGCAATCTCTTCTCCAACATTAACAATGCTCTAGATGGAGCTCCACTCCGATATGAAGAGAAGAGGCTCAAGTGGACTAACTATGAGGCCGTGATTTACGCCGCTGGTCCATTGGCTTTCCGTCCTGACCACTGCCCTGCGACTGCACCACCCACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

183

Amino Acids

20.17

Weight (kDa)

8.5

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 45 - 143 3.1e-25 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 53, 99, 128, 492
AclWI GGATC 1 cut(s) 181
AcoI YGGCCR 3 cut(s) 60, 186, 286
AcsI RAATTY 1 cut(s) 167
AfaI GTAC 1 cut(s) 149
AgsI TTSAA 1 cut(s) 316
AluBI AGCT 5 cut(s) 200, 212, 304, 367, 431
AluI AGCT 5 cut(s) 200, 212, 304, 367, 431
Alw21I GWGCWC 2 cut(s) 306, 433
Alw26I GTCTC 1 cut(s) 376
AlwI GGATC 1 cut(s) 181
AoxI GGCC 4 cut(s) 60, 186, 286, 477
ApoI RAATTY 1 cut(s) 167
Asp700I GAANNNNTTC 1 cut(s) 277
AspS9I GGNCC 2 cut(s) 337, 497
AsuHPI GGTGA 1 cut(s) 311
AsuII TTCGAA 1 cut(s) 271
AvaII GGWCC 2 cut(s) 337, 497
BalI TGGCCA 1 cut(s) 62
BanII GRGCYC 2 cut(s) 306, 433
BauI CACGAG 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 393
Bbv12I GWGCWC 2 cut(s) 306, 433
BccI CCATC 2 cut(s) 348, 419
BceAI ACGGC 4 cut(s) 66, 95, 301, 464
BcoDI GTCTC 1 cut(s) 376
BfaI CTAG 1 cut(s) 422
BfmI CTRYAG 1 cut(s) 201
BisI GCNGC 2 cut(s) 129, 492
BlsI GCNGC 2 cut(s) 130, 493
Bme18I GGWCC 2 cut(s) 337, 497
BmgT120I GGNCC 2 cut(s) 337, 497
BpiI GAAGAC 1 cut(s) 393
BplI GAGNNNNNCTC 4 cut(s) 351, 383, 420, 452
Bpu14I TTCGAA 1 cut(s) 271
BpuEI CTTGAG 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bse3DI GCAATG 1 cut(s) 42
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 2 cut(s) 36, 340
BseMI GCAATG 1 cut(s) 42
BseRI GAGGAG 6 cut(s) 66, 69, 72, 75, 78, 369
BsgI GTGCAG 1 cut(s) 519
BshFI GGCC 4 cut(s) 62, 188, 288, 479
BsiHKAI GWGCWC 2 cut(s) 306, 433
BsiSI CCGG 2 cut(s) 189, 207
BsmAI GTCTC 1 cut(s) 376
BsmBI CGTCTC 1 cut(s) 376
BsnI GGCC 4 cut(s) 62, 188, 288, 479
Bsp119I TTCGAA 1 cut(s) 271
Bsp1286I GDGCHC 2 cut(s) 306, 433
Bsp143I GATC 1 cut(s) 173
BspACI CCGC 4 cut(s) 53, 99, 128, 492
BspANI GGCC 4 cut(s) 62, 188, 288, 479
BspPI GGATC 1 cut(s) 181
BspT104I TTCGAA 1 cut(s) 271
BsrDI GCAATG 1 cut(s) 42
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 1 cut(s) 173
BssSI CACGAG 1 cut(s) 381
Bst2BI CACGAG 1 cut(s) 381
Bst4CI ACNGT 2 cut(s) 121, 152
Bst6I CTCTTC 5 cut(s) 351, 379, 404, 441, 446
BstAPI GCANNNNNTGC 2 cut(s) 107, 228
BstBI TTCGAA 1 cut(s) 271
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 2 cut(s) 36, 340
BstKTI GATC 1 cut(s) 176
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 1 cut(s) 173
BstMWI GCNNNNNNNGC 3 cut(s) 59, 107, 228
BstSFI CTRYAG 1 cut(s) 201
BstV2I GAAGAC 1 cut(s) 393
BstXI CCANNNNNNTGG 1 cut(s) 296
BsuRI GGCC 4 cut(s) 62, 188, 288, 479
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 2 cut(s) 36, 340
BtsI GCAGTG 2 cut(s) 138, 520
BtsIMutI CAGTG 2 cut(s) 138, 520
Cfr13I GGNCC 2 cut(s) 337, 497
Csp6I GTAC 1 cut(s) 148
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 1 cut(s) 177
CviQI GTAC 1 cut(s) 148
DpnI GATC 1 cut(s) 175
DpnII GATC 1 cut(s) 173
EaeI YGGCCR 3 cut(s) 60, 186, 286
Eam1104I CTCTTC 5 cut(s) 351, 379, 404, 441, 446
EarI CTCTTC 5 cut(s) 351, 379, 404, 441, 446
Ecl136II GAGCTC 2 cut(s) 304, 431
Eco24I GRGCYC 2 cut(s) 306, 433
Eco47I GGWCC 2 cut(s) 337, 497
Eco53kI GAGCTC 2 cut(s) 304, 431
EcoICRI GAGCTC 2 cut(s) 304, 431
EcoT38I GRGCYC 2 cut(s) 306, 433
Esp3I CGTCTC 1 cut(s) 376
FaeI CATG 1 cut(s) 180
FaiI YATR 7 cut(s) 103, 178, 203, 240, 297, 444, 474
FatI CATG 1 cut(s) 176
Fnu4HI GCNGC 2 cut(s) 129, 492
FokI GGATG 2 cut(s) 23, 327
FriOI GRGCYC 2 cut(s) 306, 433
Fsp4HI GCNGC 2 cut(s) 129, 492
FspBI CTAG 1 cut(s) 422
GluI GCNGC 2 cut(s) 129, 492
HaeIII GGCC 4 cut(s) 62, 188, 288, 479
HapII CCGG 2 cut(s) 189, 207
Hin1II CATG 1 cut(s) 180
HinfI GANTC 4 cut(s) 5, 235, 278, 322
HpaII CCGG 2 cut(s) 189, 207
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 148, 465
Hpy188I TCNGA 2 cut(s) 173, 440
Hpy188III TCNNGA 5 cut(s) 9, 316, 383, 422, 515
Hpy8I GTNNAC 2 cut(s) 148, 465
HpyAV CCTTC 2 cut(s) 31, 131
HpyCH4III ACNGT 2 cut(s) 121, 152
HpyCH4V TGCA 3 cut(s) 231, 393, 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 107, 228
Hsp92II CATG 1 cut(s) 180
Kzo9I GATC 1 cut(s) 173
LmnI GCTCC 4 cut(s) 191, 209, 428, 436
LpnPI CCDG 7 cut(s) 120, 172, 202, 220, 480, 528, 540
MaeI CTAG 1 cut(s) 422
MalI GATC 1 cut(s) 175
MboI GATC 1 cut(s) 173
MboII GAAGA 6 cut(s) 338, 366, 391, 398, 458, 463
MhlI GDGCHC 2 cut(s) 306, 433
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 1 cut(s) 167
MluNI TGGCCA 1 cut(s) 62
MlyI GAGTC 1 cut(s) 316
MmeI TCCRAC 2 cut(s) 116, 429
Mox20I TGGCCA 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 277
MscI TGGCCA 1 cut(s) 62
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 2 cut(s) 189, 207
MwoI GCNNNNNNNGC 3 cut(s) 59, 107, 228
NdeII GATC 1 cut(s) 173
NlaIII CATG 1 cut(s) 180
NmeAIII GCCGAG 1 cut(s) 383
NspV TTCGAA 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 277
PfeI GAWTC 2 cut(s) 235, 278
PkrI GCNGC 2 cut(s) 130, 493
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
Psp124BI GAGCTC 2 cut(s) 306, 433
PspPI GGNCC 2 cut(s) 337, 497
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
RseI CAYNNNNRTG 1 cut(s) 294
SacI GAGCTC 2 cut(s) 306, 433
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 2 cut(s) 129, 492
Sau3AI GATC 1 cut(s) 173
Sau96I GGNCC 2 cut(s) 337, 497
SchI GAGTC 1 cut(s) 316
SduI GDGCHC 2 cut(s) 306, 433
SetI ASST 6 cut(s) 202, 214, 306, 313, 369, 433
SfcI CTRYAG 1 cut(s) 201
SfuI TTCGAA 1 cut(s) 271
SinI GGWCC 2 cut(s) 337, 497
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 2 cut(s) 7, 458
SmoI CTYRAG 2 cut(s) 7, 458
Sse9I AATT 1 cut(s) 167
SsiI CCGC 4 cut(s) 53, 99, 128, 492
SspMI CTAG 1 cut(s) 422
SstI GAGCTC 2 cut(s) 306, 433
TaaI ACNGT 2 cut(s) 121, 152
TaqI TCGA 1 cut(s) 271
TasI AATT 1 cut(s) 167
TatI WGTACW 1 cut(s) 147
TauI GCSGC 2 cut(s) 131, 494
TfiI GAWTC 2 cut(s) 235, 278
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 2 cut(s) 138, 527
TspDTI ATGAA 2 cut(s) 338, 459
TspGWI ACGGA 2 cut(s) 16, 500
TspRI CASTG 2 cut(s) 138, 527
VpaK11BI GGWCC 2 cut(s) 337, 497
XapI RAATTY 1 cut(s) 167
XbaI TCTAGA 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 277
XspI CTAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.