pycom07g20420

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004472
Physical Location & Seq
Reverse (-)
22517261 .. 22517791
531 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20420.1

Sequence Viewer

Length: 531 bp
ATGGCAAGGAGGAGCTTCATAATCTTCATCTCCTCCCTCCTGCTCATCTCCCTCTCCTTCCCATCCGCAACCGCCAGCGACTACTCCCCGGAAACCCCCGAGCAAACGGAGAAAGTGGTGGTGGAAGGCGTGGTGTACTGCCAGAGCTGTGACCGCTACGGAACATCGTCCTTGGCCGGGGCCGAGCCCATTCCTTCGGCGAAAATCAGCGTCATCTGCAAGAATCACAAGGGCCAAGTCAGCTTCTACAAGTCTTTTGTGGCGGACGTTAAGGGCTACTTCTACGCACAGCTTGAAGGGTTTAAAGCTGGACAACATCTTTTGGACCATCCACTCCATGGATGCAAGGTGAAGCTTGTTTCTTCTCCCCTGGCAAAGTGCAGTGAGCTCACCAATGTGAATTACGGGCTCTATGGCGCCCCGCTTCGTTACGAGGACAAGAGGTTGTGGGGGAGGAACTATGAGGCTGTTGTGTATGCTGCCGGGCCTCTGGCTTTCCGTCCTTCTTATTGCCCTACTACTCACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

177

Amino Acids

19.37

Weight (kDa)

8.69

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 41 - 139 8.1e-26 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 416
AccB7I CCANNNNNTGG 1 cut(s) 338
AciI CCGC 5 cut(s) 66, 72, 154, 263, 422
AcoI YGGCCR 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 417
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 177, 338
AgsI TTSAA 1 cut(s) 296
AjnI CCWGG 1 cut(s) 369
AleI CACNNNNGTG 1 cut(s) 395
AluBI AGCT 7 cut(s) 15, 147, 243, 292, 308, 355, 388
AluI AGCT 7 cut(s) 15, 147, 243, 292, 308, 355, 388
Alw21I GWGCWC 1 cut(s) 390
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 4 cut(s) 174, 180, 232, 485
ApeKI GCWGC 1 cut(s) 479
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 4 cut(s) 180, 232, 325, 485
AsuC2I CCSGG 3 cut(s) 89, 178, 484
AsuHPI GGTGA 2 cut(s) 361, 382
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 325
BanI GGYRCC 1 cut(s) 416
BanII GRGCYC 3 cut(s) 189, 390, 411
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 466
BccI CCATC 2 cut(s) 70, 336
BciT130I CCWGG 1 cut(s) 371
BcnI CCSGG 3 cut(s) 89, 178, 484
BfoI RGCGCY 1 cut(s) 420
BisI GCNGC 1 cut(s) 480
BlsI GCNGC 1 cut(s) 481
Bme1390I CCNGG 4 cut(s) 89, 178, 371, 484
Bme18I GGWCC 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 4 cut(s) 180, 232, 325, 485
BmiI GGNNCC 2 cut(s) 181, 418
BmrFI CCNGG 4 cut(s) 89, 178, 371, 484
BmsI GCATC 1 cut(s) 332
BpuMI CCSGG 3 cut(s) 89, 178, 484
BsaHI GRCGYC 1 cut(s) 417
BsaJI CCNNGG 5 cut(s) 87, 171, 177, 337, 369
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 338
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 5 cut(s) 87, 171, 177, 337, 369
BseGI GGATG 3 cut(s) 62, 328, 347
BseLI CCNNNNNNNGG 2 cut(s) 177, 338
BseRI GAGGAG 2 cut(s) 22, 25
BseXI GCAGC 1 cut(s) 466
BsgI GTGCAG 1 cut(s) 400
BshFI GGCC 4 cut(s) 176, 182, 234, 487
BshNI GGYRCC 1 cut(s) 416
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 3 cut(s) 89, 177, 483
BslI CCNNNNNNNGG 2 cut(s) 177, 338
BsnI GGCC 4 cut(s) 176, 182, 234, 487
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 3 cut(s) 189, 390, 411
Bsp19I CCATGG 1 cut(s) 337
BspACI CCGC 5 cut(s) 66, 72, 154, 263, 422
BspANI GGCC 4 cut(s) 176, 182, 234, 487
BspLI GGNNCC 2 cut(s) 181, 418
BspT107I GGYRCC 1 cut(s) 416
BssECI CCNNGG 5 cut(s) 87, 171, 177, 337, 369
BssNI GRCGYC 1 cut(s) 417
BssT1I CCWWGG 2 cut(s) 171, 337
Bst2UI CCWGG 1 cut(s) 371
BstACI GRCGYC 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 76
BstDSI CCRYGG 1 cut(s) 337
BstF5I GGATG 3 cut(s) 62, 328, 347
BstH2I RGCGCY 1 cut(s) 420
BstHHI GCGC 1 cut(s) 419
BstMWI GCNNNNNNNGC 3 cut(s) 153, 216, 240
BstNI CCWGG 1 cut(s) 371
BstSCI CCNGG 4 cut(s) 87, 176, 369, 482
BstV1I GCAGC 1 cut(s) 466
BsuRI GGCC 4 cut(s) 176, 182, 234, 487
BtgI CCRYGG 1 cut(s) 337
BtsCI GGATG 3 cut(s) 62, 328, 347
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 419
Cfr13I GGNCC 4 cut(s) 180, 232, 325, 485
CseI GACGC 1 cut(s) 199
Csp6I GTAC 1 cut(s) 136
CviAII CATG 1 cut(s) 338
CviQI GTAC 1 cut(s) 136
DinI GGCGCC 1 cut(s) 418
DraI TTTAAA 1 cut(s) 304
EaeI YGGCCR 1 cut(s) 174
EciI GGCGGA 1 cut(s) 278
Ecl136II GAGCTC 1 cut(s) 388
Eco130I CCWWGG 2 cut(s) 171, 337
Eco24I GRGCYC 3 cut(s) 189, 390, 411
Eco47I GGWCC 1 cut(s) 325
Eco53kI GAGCTC 1 cut(s) 388
Eco88I CYCGRG 1 cut(s) 98
EcoICRI GAGCTC 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 369
EcoT14I CCWWGG 2 cut(s) 171, 337
EcoT38I GRGCYC 3 cut(s) 189, 390, 411
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
ErhI CCWWGG 2 cut(s) 171, 337
FaeI CATG 1 cut(s) 341
FaiI YATR 5 cut(s) 20, 339, 414, 462, 477
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FatI CATG 1 cut(s) 337
FauI CCCGC 1 cut(s) 429
Fnu4HI GCNGC 1 cut(s) 480
FokI GGATG 3 cut(s) 49, 315, 354
FriOI GRGCYC 3 cut(s) 189, 390, 411
Fsp4HI GCNGC 1 cut(s) 480
GlaI GCGC 1 cut(s) 418
GluI GCNGC 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 420
HaeIII GGCC 4 cut(s) 176, 182, 234, 487
HapII CCGG 3 cut(s) 89, 177, 483
HgaI GACGC 1 cut(s) 199
HhaI GCGC 1 cut(s) 419
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 1 cut(s) 341
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 1 cut(s) 223
HpaII CCGG 3 cut(s) 89, 177, 483
HphI GGTGA 2 cut(s) 361, 382
Hpy166II GTNNAC 1 cut(s) 136
Hpy188III TCNNGA 1 cut(s) 528
Hpy8I GTNNAC 1 cut(s) 136
HpyAV CCTTC 5 cut(s) 67, 119, 204, 290, 513
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 3 cut(s) 219, 345, 381
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 216, 240
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 1 cut(s) 341
HspAI GCGC 1 cut(s) 417
KasI GGCGCC 1 cut(s) 416
LmnI GCTCC 1 cut(s) 12
Lsp1109I GCAGC 1 cut(s) 466
LweI GCATC 1 cut(s) 332
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 2 cut(s) 149, 428
MboII GAAGA 2 cut(s) 16, 354
MhlI GDGCHC 3 cut(s) 189, 390, 411
MluCI AATT 1 cut(s) 400
Mly113I GGCGCC 1 cut(s) 417
MnlI CCTC 9 cut(s) 3, 43, 47, 62, 427, 435, 447, 457, 498
MseI TTAA 2 cut(s) 270, 303
MslI CAYNNNNRTG 1 cut(s) 395
MspI CCGG 3 cut(s) 89, 177, 483
MspR9I CCNGG 4 cut(s) 89, 178, 371, 484
MvaI CCWGG 1 cut(s) 371
MwoI GCNNNNNNNGC 3 cut(s) 153, 216, 240
NarI GGCGCC 1 cut(s) 417
NciI CCSGG 3 cut(s) 89, 178, 484
NcoI CCATGG 1 cut(s) 337
NlaIII CATG 1 cut(s) 341
NlaIV GGNNCC 2 cut(s) 181, 418
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 149
OliI CACNNNNGTG 1 cut(s) 395
PfeI GAWTC 1 cut(s) 223
PflMI CCANNNNNTGG 1 cut(s) 338
PkrI GCNGC 1 cut(s) 481
PluTI GGCGCC 1 cut(s) 420
Psp124BI GAGCTC 1 cut(s) 390
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 2 cut(s) 181, 418
PspPI GGNCC 4 cut(s) 180, 232, 325, 485
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 1 cut(s) 395
SacI GAGCTC 1 cut(s) 390
SaqAI TTAA 2 cut(s) 270, 303
SatI GCNGC 1 cut(s) 480
Sau96I GGNCC 4 cut(s) 180, 232, 325, 485
ScrFI CCNGG 4 cut(s) 89, 178, 371, 484
SduI GDGCHC 3 cut(s) 189, 390, 411
SfaNI GCATC 1 cut(s) 332
SfoI GGCGCC 1 cut(s) 418
SinI GGWCC 1 cut(s) 325
SmiMI CAYNNNNRTG 1 cut(s) 395
Sse9I AATT 1 cut(s) 400
SsiI CCGC 5 cut(s) 66, 72, 154, 263, 422
SspDI GGCGCC 1 cut(s) 416
SstI GAGCTC 1 cut(s) 390
StyD4I CCNGG 4 cut(s) 87, 176, 369, 482
StyI CCWWGG 2 cut(s) 171, 337
TaiI ACGT 1 cut(s) 270
TasI AATT 1 cut(s) 400
TatI WGTACW 1 cut(s) 135
TfiI GAWTC 1 cut(s) 223
Tru1I TTAA 2 cut(s) 270, 303
Tru9I TTAA 2 cut(s) 270, 303
TscAI CASTG 1 cut(s) 388
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 2 cut(s) 7, 16
TspGWI ACGGA 3 cut(s) 122, 174, 488
TspRI CASTG 1 cut(s) 388
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.