Rw1G032480

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004472
Physical Location & Seq
Reverse (-)
60723338 .. 60730719
7382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032480.1

Sequence Viewer

Length: 579 bp
ATGGCGAGCAAGCAACTCATCCTCGTCGCGTCATCTTTTCTTCTCATTGCATTGGCCTTCTTCCCTTCCACAACCGCCACCGCATACCCTTCAAAGTCCGAAGTGCATGTGGTTGTGGAAGGCATGGTCTACTGCCAGAGATGTGACTACAGTGGATGGTCCTTAACTGGAGCTACACCACTCCCTTCGGCTAAAAGCTGTGACTACCGTGGATGGTCCTTGACCGGAGCTATCCCACTCCCTTTGGCTAAAGTCAGCGTCGTCTGCAAAAACAACATAGGCCAAGTCGGTTACTACAAGACTTTCCAAGCAAACGCTAACGGCTACGTGTATGCTGAGCTAGAAGGTAACAAAATGAACCGTGCATTGGACTATCCCCTTCAGAACTGCAGAGTGAAGCTAGTTTCTTCTCCACTTCAAAAGTGCAACCTTGCCACCAATGTCAATTATGGCATTGATGGCTCCCATCTTCGTTCTGAAGGCAAGAGTTACACGAGTCCGAATTATAATGCTGTCATATATGCTGCAGGGCCCTTTGCTTTCCGTCCTGCTCGTTGTCCTTACACTACCCGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

192

Amino Acids

20.96

Weight (kDa)

9.24

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 65 - 154 3.3e-17 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 507
AccI GTMKAC 1 cut(s) 129
AccII CGCG 1 cut(s) 29
AciI CCGC 3 cut(s) 75, 81, 571
AcuI CTGAAG 2 cut(s) 365, 498
AfiI CCNNNNNNNGG 1 cut(s) 367
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 2 cut(s) 93, 419
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 5 cut(s) 173, 198, 230, 340, 400
AluI AGCT 5 cut(s) 173, 198, 230, 340, 400
AoxI GGCC 3 cut(s) 54, 280, 530
ApaI GGGCCC 1 cut(s) 534
ApeKI GCWGC 1 cut(s) 524
AspS9I GGNCC 4 cut(s) 159, 216, 530, 531
AvaII GGWCC 2 cut(s) 159, 216
BaeGI GKGCMC 1 cut(s) 534
BanII GRGCYC 1 cut(s) 534
BauI CACGAG 1 cut(s) 493
BbvI GCAGC 1 cut(s) 511
BccI CCATC 4 cut(s) 150, 207, 452, 474
BceAI ACGGC 1 cut(s) 337
BfaI CTAG 2 cut(s) 341, 401
BfmI CTRYAG 3 cut(s) 148, 388, 525
BisI GCNGC 1 cut(s) 525
BlpI GCTNAGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 526
Bme18I GGWCC 2 cut(s) 159, 216
BmgT120I GGNCC 4 cut(s) 159, 216, 530, 531
BmiI GGNNCC 2 cut(s) 463, 532
BpmI CTGGAG 1 cut(s) 189
Bpu1102I GCTNAGC 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 208
BsaWI WCCGGW 1 cut(s) 224
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 3 cut(s) 18, 161, 218
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 172
BseSI GKGCMC 1 cut(s) 534
BseXI GCAGC 1 cut(s) 511
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 3 cut(s) 56, 282, 532
BsiSI CCGG 1 cut(s) 225
BslI CCNNNNNNNGG 1 cut(s) 367
BsnI GGCC 3 cut(s) 56, 282, 532
Bsp120I GGGCCC 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 534
Bsp1720I GCTNAGC 1 cut(s) 336
BspACI CCGC 3 cut(s) 75, 81, 571
BspANI GGCC 3 cut(s) 56, 282, 532
BspCNI CTCAG 1 cut(s) 328
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 2 cut(s) 463, 532
BspMAI CTGCAG 2 cut(s) 392, 529
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 1 cut(s) 208
BssSI CACGAG 1 cut(s) 493
Bst2BI CACGAG 1 cut(s) 493
Bst4CI ACNGT 3 cut(s) 152, 209, 362
BstBAI YACGTR 1 cut(s) 328
BstC8I GCNNGC 2 cut(s) 7, 11
BstDEI CTNAG 1 cut(s) 336
BstDSI CCRYGG 1 cut(s) 208
BstF5I GGATG 3 cut(s) 18, 161, 218
BstFNI CGCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 2 cut(s) 264, 459
BstNSI RCATGY 1 cut(s) 110
BstSFI CTRYAG 3 cut(s) 148, 388, 525
BstSLI GKGCMC 1 cut(s) 534
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 511
BsuRI GGCC 3 cut(s) 56, 282, 532
BtgI CCRYGG 1 cut(s) 208
BtsCI GGATG 3 cut(s) 18, 161, 218
BtsIMutI CAGTG 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 7, 11
Cfr13I GGNCC 4 cut(s) 159, 216, 530, 531
CseI GACGC 2 cut(s) 18, 247
CspCI CAANNNNNGTGG 2 cut(s) 225, 260
CviAII CATG 2 cut(s) 107, 124
DdeI CTNAG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 534
Eco47I GGWCC 2 cut(s) 159, 216
Eco57I CTGAAG 2 cut(s) 365, 498
EcoO109I RGGNCCY 2 cut(s) 530, 531
EcoT38I GRGCYC 1 cut(s) 534
FaeI CATG 2 cut(s) 110, 127
FatI CATG 2 cut(s) 106, 123
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 3 cut(s) 5, 168, 225
FriOI GRGCYC 1 cut(s) 534
Fsp4HI GCNGC 1 cut(s) 525
FspBI CTAG 2 cut(s) 341, 401
GluI GCNGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 189
HaeIII GGCC 3 cut(s) 56, 282, 532
HapII CCGG 1 cut(s) 225
HgaI GACGC 2 cut(s) 18, 247
Hin1II CATG 2 cut(s) 110, 127
HinfI GANTC 1 cut(s) 496
HpaII CCGG 1 cut(s) 225
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 4 cut(s) 100, 384, 478, 501
Hpy8I GTNNAC 1 cut(s) 130
Hpy99I CGWCG 2 cut(s) 29, 263
HpyAV CCTTC 8 cut(s) 67, 75, 99, 113, 195, 338, 389, 473
HpyCH4III ACNGT 3 cut(s) 152, 209, 362
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 7 cut(s) 50, 106, 267, 365, 390, 426, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 264, 459
HpyF3I CTNAG 1 cut(s) 336
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 2 cut(s) 110, 127
LmnI GCTCC 3 cut(s) 170, 227, 467
LpnPI CCDG 5 cut(s) 149, 153, 238, 513, 561
Lsp1109I GCAGC 1 cut(s) 511
MaeI CTAG 2 cut(s) 341, 401
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 5 cut(s) 143, 200, 290, 347, 488
MboII GAAGA 4 cut(s) 32, 52, 399, 461
MhlI GDGCHC 1 cut(s) 534
MluCI AATT 2 cut(s) 445, 502
MlyI GAGTC 1 cut(s) 505
MnlI CCTC 1 cut(s) 32
MseI TTAA 2 cut(s) 164, 577
MspI CCGG 1 cut(s) 225
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 2 cut(s) 264, 459
NlaIII CATG 2 cut(s) 110, 127
NlaIV GGNNCC 2 cut(s) 463, 532
NmuCI GTSAC 2 cut(s) 143, 200
NspI RCATGY 1 cut(s) 110
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 1 cut(s) 504
PpsI GAGTC 1 cut(s) 504
Ppu21I YACGTR 1 cut(s) 328
PsiI TTATAA 1 cut(s) 507
PspN4I GGNNCC 2 cut(s) 463, 532
PspOMI GGGCCC 1 cut(s) 530
PspPI GGNCC 4 cut(s) 159, 216, 530, 531
PstI CTGCAG 2 cut(s) 392, 529
SaqAI TTAA 2 cut(s) 164, 577
SatI GCNGC 1 cut(s) 525
Sau96I GGNCC 4 cut(s) 159, 216, 530, 531
SchI GAGTC 1 cut(s) 505
SduI GDGCHC 1 cut(s) 534
SetI ASST 8 cut(s) 175, 200, 232, 330, 342, 349, 402, 432
SfcI CTRYAG 3 cut(s) 148, 388, 525
SinI GGWCC 2 cut(s) 159, 216
Sse9I AATT 2 cut(s) 445, 502
SsiI CCGC 3 cut(s) 75, 81, 571
SspMI CTAG 2 cut(s) 341, 401
TaaI ACNGT 3 cut(s) 152, 209, 362
TaiI ACGT 1 cut(s) 330
TasI AATT 2 cut(s) 445, 502
Tru1I TTAA 2 cut(s) 164, 577
Tru9I TTAA 2 cut(s) 164, 577
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 2 cut(s) 143, 200
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 2 cut(s) 143, 200
TspDTI ATGAA 1 cut(s) 371
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 1 cut(s) 157
VpaK11BI GGWCC 2 cut(s) 159, 216
XceI RCATGY 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 2 cut(s) 341, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.