FvH4_7g24940

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004472
Physical Location & Seq
Reverse (-)
19148517 .. 19149035
519 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24940.t1

Sequence Viewer

Length: 519 bp
ATGGCAGGCAGGCAGCTTATAGTCCTTGTGTCATCCCTCCTCCTCACTGCACTCGCCTTCTTCCCTTCCACAACTGCAACCGAATATCAGAAAAAAATCGATGTGGTTGTGGAGGGCATGGTGTACTGCCAGAGCTGTGACCACTATGGAACATGGTCCATGACCGGAGCTAAACCAATCCCTTCCGCTAAAGTGAGTGTCATCTGCAAGAACCACAAGGACCAAGTCAGCTTCTACAAGGCTTTCCAAACAAATGCTGATGGCTACTTCTATGCACCCCTTGATGGCTTCAAAATGAACCACATGTTGGACCATCCCCTCCAGGCTTGCCATGTCAAACCTGTCTCTTCACCTCTCGAGGATTGCAGGTTCCTCAGTAATGTCAACTATGGCCTCAATGGAGCTCCGCTTCGTTATGAAGACAAGAGAGTTATGGGAAGCAATTATGAAGCTGTGGTGTATTCTGCAGGGCCTTTGGCTTTTCGCCCTCAGCATTGTGCTCCAAAAACTCACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

173

Amino Acids

19.16

Weight (kDa)

8.48

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 37 - 134 8.6e-27 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 357
AccB7I CCANNNNNTGG 1 cut(s) 307
AciI CCGC 2 cut(s) 186, 407
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 2 cut(s) 284, 307
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 1 cut(s) 292
AjnI CCWGG 1 cut(s) 321
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AloI GAACNNNNNNTCC 2 cut(s) 353, 385
AluBI AGCT 6 cut(s) 16, 135, 170, 231, 404, 452
AluI AGCT 6 cut(s) 16, 135, 170, 231, 404, 452
Alw21I GWGCWC 2 cut(s) 406, 502
Alw26I GTCTC 1 cut(s) 349
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 2 cut(s) 391, 470
ApeKI GCWGC 1 cut(s) 13
AspS9I GGNCC 4 cut(s) 156, 220, 310, 470
AsuHPI GGTGA 1 cut(s) 342
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 3 cut(s) 156, 220, 310
BanII GRGCYC 1 cut(s) 406
BbsI GAAGAC 1 cut(s) 426
Bbv12I GWGCWC 2 cut(s) 406, 502
BbvCI CCTCAGC 1 cut(s) 489
BbvI GCAGC 1 cut(s) 25
BccI CCATC 3 cut(s) 254, 278, 321
BciT130I CCWGG 1 cut(s) 323
BcoDI GTCTC 1 cut(s) 349
BfmI CTRYAG 1 cut(s) 465
BfuAI ACCTGC 1 cut(s) 357
BisI GCNGC 1 cut(s) 14
BlsI GCNGC 1 cut(s) 15
Bme1390I CCNGG 1 cut(s) 323
Bme18I GGWCC 3 cut(s) 156, 220, 310
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 4 cut(s) 156, 220, 310, 470
BmiI GGNNCC 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 305
Bpu10I CCTNAGC 1 cut(s) 489
Bsa29I ATCGAT 1 cut(s) 99
BsaWI WCCGGW 1 cut(s) 164
Bsc4I CCNNNNNNNGG 2 cut(s) 284, 307
BseBI CCWGG 1 cut(s) 323
BseCI ATCGAT 1 cut(s) 99
BseGI GGATG 2 cut(s) 32, 313
BseLI CCNNNNNNNGG 2 cut(s) 284, 307
BseMII CTCAG 2 cut(s) 388, 503
BseRI GAGGAG 2 cut(s) 29, 32
BseXI GCAGC 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 2 cut(s) 393, 472
BshVI ATCGAT 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 406, 502
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 1 cut(s) 165
BslI CCNNNNNNNGG 2 cut(s) 284, 307
BsmAI GTCTC 1 cut(s) 349
BsnI GGCC 2 cut(s) 393, 472
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 406, 502
BspACI CCGC 2 cut(s) 186, 407
BspANI GGCC 2 cut(s) 393, 472
BspCNI CTCAG 2 cut(s) 387, 502
BspDI ATCGAT 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 371
BspMAI CTGCAG 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 357
Bst2UI CCWGG 1 cut(s) 323
Bst6I CTCTTC 1 cut(s) 352
BstC8I GCNNGC 3 cut(s) 7, 11, 328
BstDEI CTNAG 2 cut(s) 374, 489
BstF5I GGATG 2 cut(s) 32, 313
BstMAI GTCTC 1 cut(s) 349
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 465
BstV1I GCAGC 1 cut(s) 25
BstV2I GAAGAC 1 cut(s) 426
Bsu15I ATCGAT 1 cut(s) 99
BsuRI GGCC 2 cut(s) 393, 472
BsuTUI ATCGAT 1 cut(s) 99
BtsCI GGATG 2 cut(s) 32, 313
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 45
BveI ACCTGC 1 cut(s) 357
Cac8I GCNNGC 3 cut(s) 7, 11, 328
Cfr13I GGNCC 4 cut(s) 156, 220, 310, 470
ClaI ATCGAT 1 cut(s) 99
Csp6I GTAC 1 cut(s) 124
CviAII CATG 5 cut(s) 118, 153, 160, 304, 332
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 2 cut(s) 374, 489
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
Ecl136II GAGCTC 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 406
Eco47I GGWCC 3 cut(s) 156, 220, 310
Eco53kI GAGCTC 1 cut(s) 404
Eco88I CYCGRG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 406
FaeI CATG 5 cut(s) 121, 156, 163, 307, 335
FatI CATG 5 cut(s) 117, 152, 159, 303, 331
Fnu4HI GCNGC 1 cut(s) 14
FokI GGATG 2 cut(s) 19, 300
FriOI GRGCYC 1 cut(s) 406
Fsp4HI GCNGC 1 cut(s) 14
GluI GCNGC 1 cut(s) 14
GsuI CTGGAG 1 cut(s) 305
HaeIII GGCC 2 cut(s) 393, 472
HapII CCGG 1 cut(s) 165
Hin1II CATG 5 cut(s) 121, 156, 163, 307, 335
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HpaII CCGG 1 cut(s) 165
HphI GGTGA 1 cut(s) 342
Hpy166II GTNNAC 2 cut(s) 124, 385
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 2 cut(s) 124, 385
HpyAV CCTTC 3 cut(s) 67, 75, 192
HpyCH4V TGCA 6 cut(s) 50, 77, 207, 275, 366, 467
HpyF3I CTNAG 2 cut(s) 374, 489
Hsp92II CATG 5 cut(s) 121, 156, 163, 307, 335
LmnI GCTCC 4 cut(s) 167, 401, 409, 505
LpnPI CCDG 7 cut(s) 143, 178, 308, 335, 352, 354, 453
Lsp1109I GCAGC 1 cut(s) 25
MaeIII GTNAC 1 cut(s) 137
MboII GAAGA 3 cut(s) 52, 339, 431
MhlI GDGCHC 2 cut(s) 406, 502
MluCI AATT 1 cut(s) 442
MmeI TCCRAC 1 cut(s) 288
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 323
MvaI CCWGG 1 cut(s) 323
NlaIII CATG 5 cut(s) 121, 156, 163, 307, 335
NlaIV GGNNCC 1 cut(s) 371
NmuCI GTSAC 1 cut(s) 137
NspI RCATGY 1 cut(s) 307
PaeR7I CTCGAG 1 cut(s) 356
PciI ACATGT 1 cut(s) 303
PflFI GACNNNGTC 1 cut(s) 224
PflMI CCANNNNNTGG 1 cut(s) 307
PkrI GCNGC 1 cut(s) 15
PscI ACATGT 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 406
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 371
PspPI GGNCC 4 cut(s) 156, 220, 310, 470
PstI CTGCAG 1 cut(s) 469
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SacI GAGCTC 1 cut(s) 406
SatI GCNGC 1 cut(s) 14
Sau96I GGNCC 4 cut(s) 156, 220, 310, 470
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 2 cut(s) 406, 502
SetI ASST 9 cut(s) 18, 137, 172, 233, 343, 355, 371, 406, 454
SfcI CTRYAG 1 cut(s) 465
Sfr274I CTCGAG 1 cut(s) 356
SinI GGWCC 3 cut(s) 156, 220, 310
SlaI CTCGAG 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 1 cut(s) 442
SsiI CCGC 2 cut(s) 186, 407
SstI GAGCTC 1 cut(s) 406
StyD4I CCNGG 1 cut(s) 321
TaqI TCGA 2 cut(s) 99, 357
TasI AATT 1 cut(s) 442
TatI WGTACW 1 cut(s) 123
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 3 cut(s) 311, 432, 462
TspRI CASTG 1 cut(s) 52
Tth111I GACNNNGTC 1 cut(s) 224
Van91I CCANNNNNTGG 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 156, 220, 310
XceI RCATGY 1 cut(s) 307
XhoI CTCGAG 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.