Rroxscaffold_4G00286210

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004472
Physical Location & Seq
Forward (+)
7329090 .. 7329608
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286210.1

Sequence Viewer

Length: 519 bp
ATGGCAGGCAGGCAGCTTATAGTCCTCGTTTCGTCGCTCCTCCTCACCGCACTCGCCTTCTTCCCTTCCACAACCGCGACCGAACATCAGAAAAAGATTGACGTGGTTGTGGAAGGCATGGTGTACTGCCAGAGCTGTGATCACTCCGGAACATGGTCCATGACCGGGGCTAAACCAATCCCTTCGGCTAAAATCAGTGTCATCTGCAAGAACCATAAGGACCAAGTTAGCTTCTACAAGGCTTTCCAAACAAATGCTGATGGCTACTTCTATGCACACCTTGATGGCTTCAAAATGAACCACATGTTGGACCATCCCCTCCAGGCTTGCCATGTCAAACCGGTCTCTTCACCTCTCGAAGACTGCAGGTTTCTCAGCAACATCAACTATGGCCTCTCTGGAGCTCCACTTCGTTATGAAGACAAGAGAGTGATGGGAAGCAGTTATGAAGCTGTGGTGTTTTCTGCAGGGCCTTTGGCTTTTCGCCCTCTGTCTTGCGCTCCAAAAACTCACTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

172

Amino Acids

19.0

Weight (kDa)

8.51

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 37 - 134 1.3e-26 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 357
AccB7I CCANNNNNTGG 1 cut(s) 307
AccII CGCG 1 cut(s) 77
AccIII TCCGGA 1 cut(s) 146
AciI CCGC 2 cut(s) 48, 75
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 3 cut(s) 153, 165, 307
AflIII ACRYGT 1 cut(s) 303
AgeI ACCGGT 1 cut(s) 340
AgsI TTSAA 1 cut(s) 292
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 1 cut(s) 321
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AluBI AGCT 5 cut(s) 16, 135, 231, 404, 452
AluI AGCT 5 cut(s) 16, 135, 231, 404, 452
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 1 cut(s) 349
Aor13HI TCCGGA 1 cut(s) 146
AoxI GGCC 2 cut(s) 391, 470
ApeKI GCWGC 1 cut(s) 13
AsiGI ACCGGT 1 cut(s) 340
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 4 cut(s) 156, 220, 310, 470
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 37, 342
AvaII GGWCC 3 cut(s) 156, 220, 310
BanII GRGCYC 1 cut(s) 406
BbsI GAAGAC 2 cut(s) 366, 426
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 1 cut(s) 25
BccI CCATC 4 cut(s) 254, 278, 321, 427
BciT130I CCWGG 1 cut(s) 323
BclI TGATCA 1 cut(s) 139
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 349
BfmI CTRYAG 2 cut(s) 364, 465
BfuAI ACCTGC 1 cut(s) 357
BisI GCNGC 1 cut(s) 14
BlsI GCNGC 1 cut(s) 15
Bme1390I CCNGG 2 cut(s) 166, 323
Bme18I GGWCC 3 cut(s) 156, 220, 310
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 4 cut(s) 156, 220, 310, 470
BmrFI CCNGG 2 cut(s) 166, 323
BpiI GAAGAC 2 cut(s) 366, 426
BpmI CTGGAG 2 cut(s) 305, 420
BpuMI CCSGG 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 165
BsaWI WCCGGW 2 cut(s) 146, 340
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 165, 307
Bse118I RCCGGY 1 cut(s) 340
BseAI TCCGGA 1 cut(s) 146
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 1 cut(s) 313
BseLI CCNNNNNNNGG 3 cut(s) 153, 165, 307
BseMII CTCAG 1 cut(s) 388
BseRI GAGGAG 2 cut(s) 29, 32
BseXI GCAGC 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 77
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 2 cut(s) 393, 472
BshTI ACCGGT 1 cut(s) 340
BsiEI CGRYCG 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 406
BsiSI CCGG 3 cut(s) 147, 165, 341
BslI CCNNNNNNNGG 3 cut(s) 153, 165, 307
BsmAI GTCTC 1 cut(s) 349
BsnI GGCC 2 cut(s) 393, 472
Bso31I GGTCTC 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 406
Bsp13I TCCGGA 1 cut(s) 146
Bsp143I GATC 1 cut(s) 139
BspACI CCGC 2 cut(s) 48, 75
BspANI GGCC 2 cut(s) 393, 472
BspCNI CTCAG 1 cut(s) 387
BspEI TCCGGA 1 cut(s) 146
BspFNI CGCG 1 cut(s) 77
BspMAI CTGCAG 2 cut(s) 368, 469
BspMI ACCTGC 1 cut(s) 357
BspTNI GGTCTC 1 cut(s) 349
BsrFI RCCGGY 1 cut(s) 340
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 1 cut(s) 139
Bst2UI CCWGG 1 cut(s) 323
Bst6I CTCTTC 1 cut(s) 352
BstC8I GCNNGC 3 cut(s) 7, 11, 328
BstDEI CTNAG 1 cut(s) 374
BstF5I GGATG 1 cut(s) 313
BstFNI CGCG 1 cut(s) 77
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 1 cut(s) 142
BstMAI GTCTC 1 cut(s) 349
BstMBI GATC 1 cut(s) 139
BstMCI CGRYCG 1 cut(s) 81
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 2 cut(s) 164, 321
BstSFI CTRYAG 2 cut(s) 364, 465
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 25
BstV2I GAAGAC 2 cut(s) 366, 426
BsuRI GGCC 2 cut(s) 393, 472
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 313
BtsIMutI CAGTG 1 cut(s) 202
BveI ACCTGC 1 cut(s) 357
Cac8I GCNNGC 3 cut(s) 7, 11, 328
CfoI GCGC 1 cut(s) 500
Cfr10I RCCGGY 1 cut(s) 340
Cfr13I GGNCC 4 cut(s) 156, 220, 310, 470
Csp6I GTAC 1 cut(s) 124
CspAI ACCGGT 1 cut(s) 340
CviAII CATG 5 cut(s) 118, 153, 160, 304, 332
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 374
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
Ecl136II GAGCTC 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 406
Eco31I GGTCTC 1 cut(s) 349
Eco47I GGWCC 3 cut(s) 156, 220, 310
Eco53kI GAGCTC 1 cut(s) 404
EcoICRI GAGCTC 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 406
FaeI CATG 5 cut(s) 121, 156, 163, 307, 335
FatI CATG 5 cut(s) 117, 152, 159, 303, 331
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 14
FokI GGATG 1 cut(s) 300
FriOI GRGCYC 1 cut(s) 406
Fsp4HI GCNGC 1 cut(s) 14
GlaI GCGC 1 cut(s) 499
GluI GCNGC 1 cut(s) 14
GsuI CTGGAG 2 cut(s) 305, 420
HaeIII GGCC 2 cut(s) 393, 472
HapII CCGG 3 cut(s) 147, 165, 341
HhaI GCGC 1 cut(s) 500
Hin1II CATG 5 cut(s) 121, 156, 163, 307, 335
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HpaII CCGG 3 cut(s) 147, 165, 341
HphI GGTGA 2 cut(s) 37, 342
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 3 cut(s) 147, 356, 399
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 4 cut(s) 67, 75, 107, 192
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 4 cut(s) 207, 275, 366, 467
HpyF3I CTNAG 1 cut(s) 374
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 5 cut(s) 121, 156, 163, 307, 335
HspAI GCGC 1 cut(s) 498
Kpn2I TCCGGA 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 1 cut(s) 139
LmnI GCTCC 4 cut(s) 42, 401, 409, 505
LpnPI CCDG 9 cut(s) 143, 160, 178, 308, 335, 352, 354, 384, 453
Lsp1109I GCAGC 1 cut(s) 25
MaeII ACGT 1 cut(s) 102
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MboII GAAGA 4 cut(s) 52, 339, 371, 431
MhlI GDGCHC 1 cut(s) 406
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 7 cut(s) 35, 50, 53, 329, 363, 404, 498
MroI TCCGGA 1 cut(s) 146
MslI CAYNNNNRTG 1 cut(s) 282
MspI CCGG 3 cut(s) 147, 165, 341
MspR9I CCNGG 2 cut(s) 166, 323
MvaI CCWGG 1 cut(s) 323
MvnI CGCG 1 cut(s) 77
NciI CCSGG 1 cut(s) 166
NdeII GATC 1 cut(s) 139
NlaIII CATG 5 cut(s) 121, 156, 163, 307, 335
NspI RCATGY 1 cut(s) 307
PciI ACATGT 1 cut(s) 303
PflMI CCANNNNNTGG 1 cut(s) 307
PinAI ACCGGT 1 cut(s) 340
PkrI GCNGC 1 cut(s) 15
PscI ACATGT 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 406
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspPI GGNCC 4 cut(s) 156, 220, 310, 470
PstI CTGCAG 2 cut(s) 368, 469
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 282
SacI GAGCTC 1 cut(s) 406
SatI GCNGC 1 cut(s) 14
Sau3AI GATC 1 cut(s) 139
Sau96I GGNCC 4 cut(s) 156, 220, 310, 470
ScrFI CCNGG 2 cut(s) 166, 323
SduI GDGCHC 1 cut(s) 406
SetI ASST 9 cut(s) 18, 105, 137, 233, 282, 355, 371, 406, 454
SfcI CTRYAG 2 cut(s) 364, 465
SinI GGWCC 3 cut(s) 156, 220, 310
SmiMI CAYNNNNRTG 1 cut(s) 282
SsiI CCGC 2 cut(s) 48, 75
SstI GAGCTC 1 cut(s) 406
StyD4I CCNGG 2 cut(s) 164, 321
TaiI ACGT 1 cut(s) 105
TaqI TCGA 1 cut(s) 357
TaqII GACCGA 1 cut(s) 95
TatI WGTACW 1 cut(s) 123
TscAI CASTG 1 cut(s) 202
TseI GCWGC 1 cut(s) 13
TspDTI ATGAA 3 cut(s) 311, 432, 462
TspRI CASTG 1 cut(s) 202
Van91I CCANNNNNTGG 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 156, 220, 310
XceI RCATGY 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.