MD01G1156800.v1.1

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004472
Physical Location & Seq
Reverse (-)
26424270 .. 26424800
531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1156800.v1.1.491

Sequence Viewer

Length: 531 bp
ATGGCAAGCAGGAGTCTTCTAATCTTCATCTCCTCCCTCCTCCTCATCTCCCTCTCCTTCCCATCCGCAACCGCCACCGACTACTCCCTGAAAACCCCCGAGCAACCGGACCATGTGGTGGTGGAAGGCGTGGTGTATTGCCAGAGCTGTGACCACTACGGAACATGGTCCTTGACCGAGGCTGAGCCCATTCCGTCGGCAAAAATCAGCGTCATCTGCAAGGACCACAAGGGCCAGGTGAGCTTCTACAAGTCTTTCGTGACGGACGTCAAGGGCTACTTCTATGCACAGCTTGATGGCTTCAAAATTGGACAACATCTTTTGGACCATCCTCTTCATGGATGCAAAGTGAAGCTTGTTTCTTCTCCTCTGGAAAAGTGCAGTGAGCTCAGCAATGTGAATTACGGGCTCTATGGCGCCCCGCTTCGTTACGAGGACAAGAGGTTGTGGGGGAGGAACTATGAGGCTGTTGTTTATGCTGCAGGGCCCCTGGCTTTCCGTCCTTCTAACTGCCCTACTACTCACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

177

Amino Acids

19.53

Weight (kDa)

7.03

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 41 - 139 4.4e-27 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 416
AccB7I CCANNNNNTGG 1 cut(s) 118
AciI CCGC 3 cut(s) 66, 72, 422
AcyI GRCGYC 2 cut(s) 267, 417
AfiI CCNNNNNNNGG 2 cut(s) 118, 338
AgsI TTSAA 1 cut(s) 304
AjnI CCWGG 2 cut(s) 234, 489
AluBI AGCT 5 cut(s) 147, 243, 292, 355, 388
AluI AGCT 5 cut(s) 147, 243, 292, 355, 388
Alw21I GWGCWC 1 cut(s) 390
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 2 cut(s) 232, 485
ApaI GGGCCC 1 cut(s) 489
ApeKI GCWGC 1 cut(s) 479
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 7 cut(s) 109, 168, 223, 232, 325, 485, 486
AsuHPI GGTGA 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 4 cut(s) 109, 168, 223, 325
BaeGI GKGCMC 1 cut(s) 489
BanI GGYRCC 1 cut(s) 416
BanII GRGCYC 4 cut(s) 189, 390, 411, 489
BbsI GAAGAC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 466
BccI CCATC 3 cut(s) 70, 290, 336
BciT130I CCWGG 2 cut(s) 236, 491
BfmI CTRYAG 1 cut(s) 480
BfoI RGCGCY 1 cut(s) 420
BisI GCNGC 1 cut(s) 480
BlpI GCTNAGC 2 cut(s) 183, 389
BlsI GCNGC 1 cut(s) 481
Bme1390I CCNGG 2 cut(s) 236, 491
Bme18I GGWCC 4 cut(s) 109, 168, 223, 325
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 7 cut(s) 109, 168, 223, 232, 325, 485, 486
BmiI GGNNCC 3 cut(s) 418, 487, 488
BmrFI CCNGG 2 cut(s) 236, 491
BmsI GCATC 1 cut(s) 332
BoxI GACNNNNGTC 1 cut(s) 266
BpiI GAAGAC 1 cut(s) 8
Bpu1102I GCTNAGC 2 cut(s) 183, 389
BsaHI GRCGYC 2 cut(s) 267, 417
BsaJI CCNNGG 2 cut(s) 177, 489
BsaWI WCCGGW 1 cut(s) 106
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 338
Bse3DI GCAATG 1 cut(s) 400
BseBI CCWGG 2 cut(s) 236, 491
BseDI CCNNGG 2 cut(s) 177, 489
BseGI GGATG 3 cut(s) 62, 328, 347
BseLI CCNNNNNNNGG 2 cut(s) 118, 338
BseMI GCAATG 1 cut(s) 400
BseMII CTCAG 2 cut(s) 174, 403
BseRI GAGGAG 4 cut(s) 22, 29, 32, 357
BseSI GKGCMC 1 cut(s) 489
BseXI GCAGC 1 cut(s) 466
BsgI GTGCAG 1 cut(s) 400
BshFI GGCC 2 cut(s) 234, 487
BshNI GGYRCC 1 cut(s) 416
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 1 cut(s) 107
BslI CCNNNNNNNGG 2 cut(s) 118, 338
BsnI GGCC 2 cut(s) 234, 487
BsoBI CYCGRG 1 cut(s) 98
Bsp120I GGGCCC 1 cut(s) 485
Bsp1286I GDGCHC 4 cut(s) 189, 390, 411, 489
Bsp1720I GCTNAGC 2 cut(s) 183, 389
BspACI CCGC 3 cut(s) 66, 72, 422
BspANI GGCC 2 cut(s) 234, 487
BspCNI CTCAG 2 cut(s) 175, 402
BspLI GGNNCC 3 cut(s) 418, 487, 488
BspMAI CTGCAG 1 cut(s) 484
BspT107I GGYRCC 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 400
BssECI CCNNGG 2 cut(s) 177, 489
BssNI GRCGYC 2 cut(s) 267, 417
Bst2UI CCWGG 2 cut(s) 236, 491
Bst6I CTCTTC 1 cut(s) 339
BstACI GRCGYC 2 cut(s) 267, 417
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 183, 389
BstF5I GGATG 3 cut(s) 62, 328, 347
BstH2I RGCGCY 1 cut(s) 420
BstHHI GCGC 1 cut(s) 419
BstMWI GCNNNNNNNGC 2 cut(s) 216, 240
BstNI CCWGG 2 cut(s) 236, 491
BstPAI GACNNNNGTC 1 cut(s) 266
BstSCI CCNGG 2 cut(s) 234, 489
BstSFI CTRYAG 1 cut(s) 480
BstSLI GKGCMC 1 cut(s) 489
BstV1I GCAGC 1 cut(s) 466
BstV2I GAAGAC 1 cut(s) 8
BsuRI GGCC 2 cut(s) 234, 487
BtsCI GGATG 3 cut(s) 62, 328, 347
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 419
Cfr13I GGNCC 7 cut(s) 109, 168, 223, 232, 325, 485, 486
CseI GACGC 1 cut(s) 199
CviAII CATG 3 cut(s) 113, 165, 338
DdeI CTNAG 2 cut(s) 183, 389
DinI GGCGCC 1 cut(s) 418
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 388
Eco24I GRGCYC 4 cut(s) 189, 390, 411, 489
Eco47I GGWCC 4 cut(s) 109, 168, 223, 325
Eco53kI GAGCTC 1 cut(s) 388
Eco88I CYCGRG 1 cut(s) 98
EcoICRI GAGCTC 1 cut(s) 388
EcoO109I RGGNCCY 2 cut(s) 485, 486
EcoRII CCWGG 2 cut(s) 234, 489
EcoT38I GRGCYC 4 cut(s) 189, 390, 411, 489
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
FaeI CATG 3 cut(s) 116, 168, 341
FaiI YATR 7 cut(s) 114, 166, 285, 339, 414, 462, 477
FalI AAGNNNNNCTT 4 cut(s) 263, 295, 339, 371
FatI CATG 3 cut(s) 112, 164, 337
FauI CCCGC 1 cut(s) 429
Fnu4HI GCNGC 1 cut(s) 480
FokI GGATG 3 cut(s) 49, 315, 354
FriOI GRGCYC 4 cut(s) 189, 390, 411, 489
Fsp4HI GCNGC 1 cut(s) 480
GlaI GCGC 1 cut(s) 418
GluI GCNGC 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 420
HaeIII GGCC 2 cut(s) 234, 487
HapII CCGG 1 cut(s) 107
HgaI GACGC 1 cut(s) 199
HhaI GCGC 1 cut(s) 419
Hin1I GRCGYC 2 cut(s) 267, 417
Hin1II CATG 3 cut(s) 116, 168, 341
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 1 cut(s) 13
HpaII CCGG 1 cut(s) 107
HphI GGTGA 1 cut(s) 250
Hpy188III TCNNGA 3 cut(s) 259, 371, 528
Hpy99I CGWCG 1 cut(s) 199
HpyAV CCTTC 3 cut(s) 67, 119, 513
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 5 cut(s) 219, 287, 345, 381, 482
HpyF10VI GCNNNNNNNGC 2 cut(s) 216, 240
HpyF3I CTNAG 2 cut(s) 183, 389
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 2 cut(s) 267, 417
Hsp92II CATG 3 cut(s) 116, 168, 341
HspAI GCGC 1 cut(s) 417
KasI GGCGCC 1 cut(s) 416
LpnPI CCDG 9 cut(s) 101, 120, 155, 221, 248, 356, 468, 476, 503
Lsp1109I GCAGC 1 cut(s) 466
LweI GCATC 1 cut(s) 332
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 3 cut(s) 149, 259, 428
MboII GAAGA 4 cut(s) 8, 16, 326, 354
MhlI GDGCHC 4 cut(s) 189, 390, 411, 489
MluCI AATT 2 cut(s) 306, 400
Mly113I GGCGCC 1 cut(s) 417
MlyI GAGTC 1 cut(s) 22
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 2 cut(s) 236, 491
MvaI CCWGG 2 cut(s) 236, 491
MwoI GCNNNNNNNGC 2 cut(s) 216, 240
NarI GGCGCC 1 cut(s) 417
NlaIII CATG 3 cut(s) 116, 168, 341
NlaIV GGNNCC 3 cut(s) 418, 487, 488
NmuCI GTSAC 2 cut(s) 149, 259
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 1 cut(s) 481
PleI GAGTC 1 cut(s) 21
PluTI GGCGCC 1 cut(s) 420
PpsI GAGTC 1 cut(s) 21
PshAI GACNNNNGTC 1 cut(s) 266
Psp124BI GAGCTC 1 cut(s) 390
Psp6I CCWGG 2 cut(s) 234, 489
PspGI CCWGG 2 cut(s) 234, 489
PspN4I GGNNCC 3 cut(s) 418, 487, 488
PspOMI GGGCCC 1 cut(s) 485
PspPI GGNCC 7 cut(s) 109, 168, 223, 232, 325, 485, 486
PstI CTGCAG 1 cut(s) 484
SacI GAGCTC 1 cut(s) 390
SatI GCNGC 1 cut(s) 480
Sau96I GGNCC 7 cut(s) 109, 168, 223, 232, 325, 485, 486
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 2 cut(s) 236, 491
SduI GDGCHC 4 cut(s) 189, 390, 411, 489
SetI ASST 8 cut(s) 149, 240, 245, 270, 294, 357, 390, 446
SfaNI GCATC 1 cut(s) 332
SfcI CTRYAG 1 cut(s) 480
SfoI GGCGCC 1 cut(s) 418
SinI GGWCC 4 cut(s) 109, 168, 223, 325
Sse9I AATT 2 cut(s) 306, 400
SsiI CCGC 3 cut(s) 66, 72, 422
SspDI GGCGCC 1 cut(s) 416
SstI GAGCTC 1 cut(s) 390
StyD4I CCNGG 2 cut(s) 234, 489
TaiI ACGT 1 cut(s) 270
TaqII GACCGA 1 cut(s) 191
TasI AATT 2 cut(s) 306, 400
TscAI CASTG 1 cut(s) 388
TseFI GTSAC 2 cut(s) 149, 259
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 2 cut(s) 149, 259
TspDTI ATGAA 2 cut(s) 16, 326
TspGWI ACGGA 4 cut(s) 174, 183, 278, 488
TspRI CASTG 1 cut(s) 388
Van91I CCANNNNNTGG 1 cut(s) 118
VpaK11BI GGWCC 4 cut(s) 109, 168, 223, 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.